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Plymouth was colder, whereas Jamestown was warmer and more humid, and therefore more prone to harbor diseases. I'm sure that there were a lot more differences, but those are the big ones that leapt into my mind.
Jamestown was the first permanent English colony in North America. It was located on a piece of land in the James River, near what is now Williamsburg, Virginia. The first permanent English colony in America was founded on May 14, 1607, on a peninsula of the James River in what is now the state of Virginia.