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Plymouth was colder, whereas Jamestown was warmer and more humid, and therefore more prone to harbor diseases. I'm sure that there were a lot more differences, but those are the big ones that leapt into my mind.
The colony at Jamestown seemed doomed to meet the same fate as the Roanoke Colony and the Popham Colony, two earlier failed English attempts to settle in North America, unless there was a major relief effort, despite the delivery of supplies in 1608 on the First and Second Supply missions of Captain Christopher Newport.