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   DNA ligase | definition of DNA ligase by Medical dictionary
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</a></div><div class=header-bottom><nav id=nav><ul><li class=available><a title="DNA ligase in general English dictionary"href=//www.thefreedictionary.com/DNA+ligase> <span class=cell> Dictionary </span> </a><li class=available><a title="DNA ligase in Thesaurus"href=//www.freethesaurus.com/DNA+ligase> <span class=cell> Thesaurus </span> </a><li class="active available"><div><span class=cell> Medical <br> Dictionary </span></div><li class=available><a title="DNA ligase in legal dictionary"href=//legal-dictionary.thefreedictionary.com/DNA+ligase> <span class=cell> Legal <br> Dictionary </span> </a><li><div><span class=cell> Financial <br> Dictionary </span></div><li><div><span class=cell> Acronyms </span></div><li><div><span class=cell> Idioms </span></div><li class=available><a title="DNA ligase in encyclopedia"href=//encyclopedia2.thefreedictionary.com/DNA+ligase> <span class=cell> Encyclopedia </span> </a><li class=available><a title="DNA ligase in 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href=https://www.thefreedictionary.com/lookup.htm#sitecontent> Free content </a><li><a href=https://www.thefreedictionary.com/lookup.htm#link> Linking </a><li><a href=https://www.thefreedictionary.com/lookup.htm#searchbox> Lookup box </a></ul></div><a class="popup-opener style01"id=popupBoxClose onclick=showPopup(event,'popupBox','open-close-active')> Close </a></div></div></div></div></header><main id=main role=main><div class=main-holder><div class=vu id=content><div class=content-holder><div class=vu id=vu1></div><h1>DNA ligase</h1><br> Also found in: <a href=//www.thefreedictionary.com/DNA+ligase> Dictionary </a> , <a href=//www.freethesaurus.com/DNA+ligase> Thesaurus </a> , <a href=//legal-dictionary.thefreedictionary.com/DNA+ligase> Legal </a> , <a href=//encyclopedia2.thefreedictionary.com/DNA+ligase> Encyclopedia </a> , <a href=//encyclopedia.thefreedictionary.com/DNA+ligase> Wikipedia </a> . <div id=MainTxt><div id=Definition><section data-src=MillerKeane><h2>ligase</h2>  [ <span class=pronOx> li´gās, lig´ās </span> ] <div>any of a class of enzymes that catalyze the joining together of two molecules coupled with the breakdown of a pyrophosphate bond in ATP or a similar triphosphate.</div><div class=runseg><b> DNA ligase </b> either of two enzymes that join two double-helical molecules of <a href=DNA> DNA </a> together to make a longer DNA molecule.</div><div class=runseg><b> ligase chain reaction </b> a type of <a href=DNA+amplification> DNA <span style=font-variant:small-caps> amplification </span> </a> that uses <a href=DNA+ligase> DNA <span style=font-variant:small-caps> ligase </span> </a> to link chains and amplify the template containing the sequence in question.</div><div class=cprh><span class="i A cpr"> </span> Miller-Keane Encyclopedia and Dictionary of Medicine, Nursing, and Allied Health, Seventh Edition. © 2003 by Saunders, an imprint of Elsevier, Inc. All rights reserved.</div></section><section data-src=wkMed><h2>DNA li·gase</h2><div class=ds-single>an enzyme that leads to the formation of a phosphodiester bond at a break of one strand in duplex DNA; a part of the DNA repair system.</div><div class=cprh><span class="i A cpr"> </span> Farlex Partner Medical Dictionary © Farlex 2012</div></section><section data-src=hcBio><h2>DNA ligase</h2> an enzyme that catalyses the formation of covalent bonds between adjacent parts of a broken <a href=DNA> DNA </a><a href=polynucleotide+chain> POLYNUCLEOTIDE CHAIN </a> . In this way a <a href=phosphodiester+bond> PHOSPHODIESTER BOND </a> is formed between a 3'-OH group of one part of the chain and a 5'-PO <sub> 4 </sub> group of the adjacent part. <a href=DNA> DNA </a> ligase has a role in <a href=DNA> DNA </a> replication and <a href=DNA+repair> DNA REPAIR </a> . It is also used in the formation of recombinant <a href=DNA> DNA </a> molecules in <a href=genetic+engineering> GENETIC ENGINEERING </a> . <div class=cprh><span class="i A cpr"> </span> Collins Dictionary of Biology, 3rd ed. © W. G. Hale, V. A. Saunders, J. P. 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</a></div><div class="list-holder height240"><div class=lit>They will hybridize next to each other and form a substrate for <span class=highlight> DNA ligase </span> activity only when a perfect match occurs, and are particularly sensitive to disruption from any mismatch near the nick--like a single nucleotide polymorphism (SNP). <div class=lit_links><a href=https://www.thefreelibrary.com/Applications+of+ligation-mediated+PCR-a0531710792> Applications of ligation-mediated PCR </a></div></div><div class=lit>Jackson, "Mammalian DNA double-strand break repair protein XRCC4 interacts with <span class=highlight> DNA ligase </span> IV"' Current Biology, vol. <div class=lit_links><a href=https://www.thefreelibrary.com/Physiological+Roles+of+DNA+Double-Strand+Breaks-a0554287639> Physiological Roles of DNA Double-Strand Breaks </a></div></div><div class=lit>In the paper, according to RSAS, "Modrich demonstrated the requirement of DNA polymerase III, exo-nuclease I, and <span class=highlight> DNA ligase </span> for mismatch repair. <div class=lit_links><a href=https://www.thefreelibrary.com/Nobel+Prize+honors+DNA+repair+pioneers%3A+three+scientists+who+described...-a0436331151> Nobel Prize honors DNA repair pioneers: three scientists who described the mechanisms that protect our genetic information have been awarded the Nobel Prize in Chemistry </a></div></div><div class=lit>(1997) Mammalian DNA double-strand break repair protein XRCC4 interacts with <span class=highlight> DNA ligase </span> IV. <div class=lit_links><a href=https://www.thefreelibrary.com/Ionizing+radiation-induced+XRCC4+phosphorylation+is+mediated+through...-a0393518243> Ionizing radiation-induced XRCC4 phosphorylation is mediated through ATM in addition to DNA-PK </a></div></div><div class=lit>Hura et al., "A flexible interface between <span class=highlight> DNA ligase </span> and PCNA supports conformational switching and efficient ligation of DNA," Molecular Cell, vol. <div class=lit_links><a href=https://www.thefreelibrary.com/Archaeal+genome+guardians+give+insights+into+eukaryotic+DNA...-a0416596440> Archaeal genome guardians give insights into eukaryotic DNA replication and damage response proteins </a></div></div><div class=lit>The total volume of the LDR reaction system was 10 [micro]l including 1 [micro]l 10 x Buffer, 1 [micro]l primer and probe mixture (0.5 pM/each), 0.05 [micro]l (40 U/[micro]l) ligase (Taq <span class=highlight> DNA ligase </span> enzyme system), 1 [micro]l PCR product (100 ng/[micro]l), and 6.95 [micro]l deionized water. <div class=lit_links><a href=https://www.thefreelibrary.com/A+study+on+BMPR-IB+genes+of+Bayanbulak+sheep-a0321179426> A study on BMPR-IB genes of Bayanbulak sheep </a></div></div><div class=lit>The kit combines TurboLigase[TM], an ultrapure recombinant T4 <span class=highlight> DNA Ligase </span> , and 5x TurboLigation buffer, an optimized quick ligation buffer. <div class=lit_links><a href=https://www.thefreelibrary.com/MoBiTec+GmbH%2C+Germany%2C+is+Offering+TurboLigation%5BTM%5D+Kit%2C+Enabling...-a0312765796> MoBiTec GmbH, Germany, is Offering TurboLigation[TM] Kit, Enabling Quick Ligation of DNA Molecules in Just 5 Minutes at a Bargain Price </a></div></div><div class=lit>The amplified gene of cellulase was purified from the agarose gel after electrophoresis using Qiagen gel extraction kit and ligated into the pTZ57R/T cloning vector (Fermentas) using <span class=highlight> DNA ligase </span> and ligation buffer. <div class=lit_links><a href=https://www.thefreelibrary.com/Cloning+of+a+cellulase+gene+from+indigenous+strain+of+bacillus+species-a0292793128> Cloning of a cellulase gene from indigenous strain of bacillus species </a></div></div><div class=lit>LDR reactions were carried out in a volume of 20 [micro]L containing 20 mmol/L Tris-HCl (pH 7.6), 10 mmol/L Mg[Cl.sub.2], 100 mmol/L KCl, 10 mmol/l DTT, 1 mmol/L EDTA, 1 mmol/L NAD+, 12.5 mmol/L each probe, 3[micro]L of PCR product, and 0.1 mmol/L <span class=highlight> DNA ligase </span> . The LDR was performed in a PE 9600 thermal cycler by incubating at 95[degrees]C for 2 min and cycling for 30 cycles at 95oC for 30 s and 65[degrees]C for 4 min. <div class=lit_links><a href=https://www.thefreelibrary.com/Simultaneous+detection+of+hepatitis+B+virus+genotypes+and+mutations...-a0343948967> Simultaneous detection of hepatitis B virus genotypes and mutations associated with resistance to lamivudine, adefovir, and telbivudine by the polymerase chain reaction-ligase detection reaction </a></div></div><div class=lit>Descriptively, the hOGG1 gene on chromosome 3p26 catalyzes the cleavage of a glycosylic bond between the modified base and a sugar moiety, leaving an abasic apurinic/apyrimidinic site in DNA which is then incised followed by a few successive actions of a phosphodiesterase that completes the repair process with a final function by a DNA polymerase, and a <span class=highlight> DNA ligase </span> (7,8). <div class=lit_links><a href=https://www.thefreelibrary.com/Association+of+gene+polymorphisms+with+development+of+cancer+risk+or...-a0261151479> Association of gene polymorphisms with development of cancer risk or their protective role associated with some mutant alleles </a></div></div><div class=lit>Note exquisite sensitivity in cell strains derived from DNA damage sensing- or repair-deficient cells derived from patients with abnormal ATM, NBS1 and <span class=highlight> DNA ligase </span> IV proteins. <div class=lit_links><a href=https://www.thefreelibrary.com/The+therapeutic+ratio+is+preserved+for+radiotherapy+or+cisplatin...-a0253337204> The therapeutic ratio is preserved for radiotherapy or cisplatin treatment in BRCA2-mutated prostate cancers </a></div></div><div class=lit>In the blunt end, a <span class=highlight> DNA ligase </span> will join together the 3' end and the 5' end of one molecule with the 5' end and the 3' end, respectively, of another molecule. <div class=lit_links><a 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