The effects of ascorbic acid on the induction of urothelial lesions in mice by 2-acetylaminofluorene.
288 BALB/c male mice were allocated to: Group 1-control; Group 2-500 ppm of 2-acetylaminofluorene (2-AAF) in the food, with control water; Group 4-500 ppm of 2-AAF in the food and 250 mg/100 ml of vitamin C in the water; and Group 3-control food and 250 mg% of vitamin C in the water. Major histopathologic changes at the end of 28 days included both inflammation of the lamina propria and hyperplasia of the transitional epithelium of the urinary bladder in mice receiving 2-AAF. The most severe lesions were seen in the mice administered the combination of 2-AAF and vitamin C. It was postulated that either decreased water consumption and concentration of the urine and/or reduction in urinary pH may have contributed to the severity of the lesions.