INTRODUCTION {#sec0005}
============

Creutzfeldt-Jakob disease (CJD) and Alzheimer's disease (AD) are neurodegenerative disorders with overlapping clinical and laboratory features. CJD includes six major clinical-pathological subtypes that are largely determined by the genotype at the methionine (M)/valine (V) polymorphic codon 129 of the *PRNP* gene and the type (1 or 2) of pathological prion protein accumulating in the brain (namely MM1, MM2, MV1, MV2, VV1, and VV2) \[[@ref001]\]. While the most common MM/MV1 subtype usually manifest with a subacute clinical course, atypical sporadic CJD variants such as MV 2K, MM 2C, and VV1 show a significantly slower clinical course, which may mimic AD and other neurodegenerative dementias \[[@ref002]\]. On the other hand, although AD typically presents with a slowly progressive and well defined profile of cognitive decline, there also are well recognized variants of the disease with a rapid clinical progression and/or focal neurological signs manifesting relatively early in the disease course \[[@ref003]\]. Cerebrospinal fluid (CSF) biomarker analysis is increasingly used in the diagnostic work-up of patients with neurodegenerative dementia. In particular, the search for elevated, above threshold, levels of protein 14-3-3 and/or t-tau is recommended by diagnostic criteria for CJD \[[@ref007]\], whereas a reduced concentration of amyloid-β peptide 1--42 (Aβ~42~) in combination with increased levels of both total (t-tau) and/or phosphorylated tau (p-tau) represents a well-established neurochemical profile in support of the clinical diagnosis of AD \[[@ref009]\]. However, the lack of full sensitivity and specificity of these biomarkers still represents a significant limitation. In particular, approximately 10% of AD patients have t-tau levels compatible with CJD and/or show a positive 14-3-3 assay \[[@ref005]\], whereas a similar proportion of CJD cases, especially those of the MV2K, MM2C, and MM2T subtypes, is associated with a negative 14-3-3 test and/or with low t-tau levels \[[@ref002]\]. Finally, reduced Aβ~42~ CSF levels have also been found in CJD \[[@ref022]\]. To improve the differential diagnosis between AD and CJD based on CSF biomarkers, some studies analyzed the performance of the combined analyses of multiple protein markers including t-tau and p-tau, but also total prion protein (t-PrP) \[[@ref028]\]. Most significantly, the addition of t-PrP dosage and the calculation of the t-tau/(p-tau×t-PrP) ratio, also designated as "CJ factor", has allowed, to date, the most accurate diagnosis in AD with clinical overlap with CJD \[[@ref029]\]. However, these results have not been widely validated and there have been some divergent results between studies in terms oft-PrP CSF levels. Furthermore, the accuracy of the proposed CSF biomarker combination in the atypical variants of CJD has not been investigated as yet. In this study, we evaluated the diagnostic performance of CSF t-PrP levels either alone or in combination with other protein biomarkers, including Aβ~42~ in typical and atypical variants of both CJD and AD.

MATERIALS AND METHODS {#sec0010}
=====================

Patient characterization {#sec0015}
------------------------

We analyzed 230 CSF samples submitted to the Neuropathology Laboratory at the Institute of Neurological Sciences of Bologna, including 33 controls, 89 AD patients, and 108 CJD patients. Clinical history and follow-up data were acquired for each patient. Brain magnetic resonance imaging (MRI) studies, inclusive of fluid attenuated inversion recovery (FLAIR) and diffusion weighted imaging (DWI) sequences, and electroencephalographic (EEG) recording were also available as part of routine clinical investigations.

The diagnosis of AD was made according to the 2011 National Institute on Aging and the Alzheimer's Association workgroup guidelines \[[@ref031]\]. In particular, after a clinical follow-up of at least 24 months, all 89 AD patients fulfilled criteria for probable AD dementia with high evidence of the AD pathophysiological process. Moreover, evidence for AD pathology of high severity was obtained by neuropathological examination in 6 cases \[[@ref032]\].

CJD patients were classified according to the updated WHO diagnostic criteria \[[@ref007]\]. The largest group of "definite" CJD consisted of 83 autopsy-confirmed sporadic CJD and 8 genetic CJD, while the "probable" CJD group included 17 patients fulfilling the clinical criteria for possible CJD and showing a positive EEG and/or a positive DWI-MRI \[[@ref007]\]. All CJD cases underwent *PRNP* genetic analysis, as described previously \[[@ref033]\]. Moreover, PrP^Sc^ typing and CJD histotype classification was performed in all autopsied cases according to established methodologies and consensus criteria \[[@ref034]\]. CSF samples, in both AD and CJD groups, were selected to include a significant number of patients with an atypical clinical presentation and/or anatypical CSF biomarker profile. In particular, we defined AD as atypical/rapidly progressive (a/rpAD) when at least one of the following features was present: (1) rapid cognitive decline or presence of additional motor signs at time of CSF analyses \[[@ref029]\], (2) CSF t-tau \>1200 pg/ml, or (3) a positive CSF 14-3-3 assay, whereas for CJD the classification of atypical required at least one among: (1) clinical course \>2 years, (2) progressive cognitive decline without focal neurological signs (up to the time of CSF analyses), (3) CSF t-tau \<1200 pg/ml, (4) borderline or negative CSF 14-3-3 assay. Finally, the control group included 33 age- and sex-matched subjects lacking any clinical or neuroradiological evidence of central nervous system disease.

In our population, there were 44 AD and 54 CJD patients manifesting an atypical clinical presentation and/or showing an atypical CSF biomarkers profile. Among the remaining patients, 45 were classified as typical AD and 54 as typical CJD.

Informed consent was obtained from all subjects included in the study according to the Declaration of Helsinki.

CSF biochemical analysis {#sec0020}
------------------------

CSF samples were obtained by lumbar puncture at L3/L4 or L4/L5 levels following a standard procedure. Samples were centrifuged at 1000×g for 10 min, divided into aliquots, and stored in polypropylene tubes at --80°C until analysis.

14-3-3 protein was detected by a western blot immunoassay using CSF controls with a weak or a strong 14-3-3 signal, respectively, as internal quality controls. The immunoreactivity signals were classified as negative when the 14-3-3 optical densitometric (OD) band was lower than the weakly positive control; borderline (or weakly positive), when the 14-3-3 OD was up to two times higher than the control, and positive when it was at least two times higher than the control \[[@ref036]\].

We measured CSF t-tau, p-tau, and Aβ~42,~ levels using commercially available enzyme-linked immunosorbent assay (ELISA) kits (INNOTEST htau-Ag, INNOTEST phosphorylated-Tau181, and INNOTEST Aβ1--42; Innogenetics/Fujirebio Europe) according to the manufacturer's instructions. T-PrP CSF levels were determined using commercial Beta Prion Human Enzyme-Linked Immunoassay Test kits (AJ Roboscreen, Leipzig, Germany) according to the manufacturer's instructions.

Statistical analyses {#sec0025}
--------------------

Statistical analysis was performed using SPSS software (version 21- IBM Analytics). Several combinations of biomarkers were analyzed. Depending on the data distribution, the Kruskal-Wallis test and the Mann-Whitney *U* test or the one-way ANOVA (followed by Tukey's *post hoc* test) were used to test differences between AD patients, CJD patients, and controls regarding demographic data and biomarkers measurements. Data were expressed as mean±standard deviation (SD) or median and interquartile range (IQR). A Bonferroni correction was applied to multiple comparisons. Receiver operating characteristic analyses (ROC) were performed to establish the diagnostic accuracy, sensitivity, and specificity and the optimal cut-off value of each biomarker or combination of biomarkers. Differences were considered statistically significant at *p* \< 0.05.

RESULTS {#sec0030}
=======

Demographic characteristics {#sec0035}
---------------------------

In the control, AD, and CJD groups, mean ages and standard deviations were 61.6±10.9, 66.4±9.1, 66.4±9.0 years, respectively. Regarding sex, 42.4% controls, 59.6% AD patients, and 50.9% CJD patients were female. The molecular classification of CJD cases and their disease duration are summarized in [Table 1](#jad-55-jad160740-t001){ref-type="table"}. Atypical CJD cases presented a longer duration of the disease than typical CJD cases (*p* \< 0.001). [Table 2](#jad-55-jad160740-t002){ref-type="table"} shows the clinical and laboratory features of cases classified as a/rpAD.

CSF t-PrP levels ([Fig. 1](#jad-55-jad160740-g001){ref-type="fig"}) {#sec0040}
-------------------------------------------------------------------

CSF t-PrP levels were significantly lower in CJD patients compared to AD patients (*p* \< 0.001) and controls (*p* \< 0.001), but did not differ significantly between AD and controls (*p* = 0.500). A value of t-PrP lower than 261 ng/ml distinguished CJD patients from AD patients with a 73.0% sensitivity and a 75.0% specificity. Samples of typical CJD cases showed higher CSF t-PrP levels than those of the atypical group (*p* = 0.005).

CSF biomarker values in AD and CJD {#sec0045}
----------------------------------

CSF biomarker data in the AD and CJD groups are summarized in [Table 3](#jad-55-jad160740-t003){ref-type="table"}.

There were statistically significant differences between CJD (all) and AD (all) patients regarding t-tau (*p* \< 0.001), 14-3-3 (*p* \< 0.001), Aβ~42~ (*p* \< 0.001), t-tau/p-tau ratio (*p* \< 0.001), t-tau/t-PrP (*p* \< 0.001), Aβ~42~/p-tau (*p* \< 0.001), Aβ~42~/(p-tau×t-PrP) (*p* \< 0.001), Aβ~42~×t-tau/p-tau (*p* \< 0.001), CJ factor (*p* \< 0.001), and (Aβ~42~×t-tau)/(p-tau×t-PrP) (*p* \< 0.001).

Results from ROC analysis for biomarker combinations are illustrated in [Table 4](#jad-55-jad160740-t004){ref-type="table"} and [Fig. 2](#jad-55-jad160740-g002){ref-type="fig"}. The (t-tau×Aβ~42~)/(p-tau×t-PrP) ratio achieved the best accuracy (area under the curve (AUC) = 0.995) in the discrimination of CJD from AD with 98.1% sensitivity and 97.7% specificity, using a cut-off value of 24.0. The accuracy, cut-off values, sensitivity and specificity of the other biomarker combinations are illustrated in [Table 4](#jad-55-jad160740-t004){ref-type="table"}.

Subgroup analysis {#sec0050}
-----------------

ROC analyses were also performed for the following comparisons: atypical CJD versus atypical/rapidly progressive AD; typical CJD versus atypical/rapidly progressive AD; atypical CJD versus typical AD.

Atypical CJD versus atypical/rapidly progressive AD {#sec0055}
---------------------------------------------------

[Table 5](#jad-55-jad160740-t005){ref-type="table"} illustrates the biomarker accuracy, cut-off values, sensitivity, and specificity for the comparison between atypical CJD and a/rpAD groups. There were no significant differences in CSF t-tau levels and 14-3-3 assay results between a/rp AD and atypical CJD (*p* = 0.057 and *p* = 0.323, respectively). In contrast, values of Aβ~42~, t-tau/p-tau ratio, t-tau/t-PrP, Aβ~42~/p-tau, Aβ~42~/(p-tau×t-PrP), Aβ~42~×t-tau/p-tau, CJ factor, (Aβ~42~×t-tau)/(p-tau×t-PrP) significantly differed between the two groups (*p* \< 0.001). While the 1200 pg/ml cut-off and 14-3-3 test yielded only 61.1% and 25.9% sensitivity and 50.0% and 84.1% specificity, respectively, in the differential diagnosis, the (t-tau×Aβ~42~)/(p-tau×t-PrP) ratio best distinguished (AUC = 0.986) atypical CJD from a/rp AD with 96.2% sensitivity and 95.5% specificity with an optimal cut-off value of 24. A relatively good sensitivity and specificity, with values above 90%, was also obtained for three out four biomarkers (t-tau and p-tau plus t-PrP or Aβ~42~).

Typical CJD versus atypical/rapidly progressive AD and atypical CJD versus typical AD {#sec0060}
-------------------------------------------------------------------------------------

The biomarkers accuracy, cut-off values, sensitivity, and specificity for these comparisons are reported in [Supplementary Tables 1](#S1){ref-type="supplementary-material"} and [2](#S1){ref-type="supplementary-material"}. While the t-tau×Aβ~42~/p-tau ratio yielded the higher accuracy with 100% sensitivity and 97.7% specificity in the first comparison, the (t-tau×Aβ~42~)/(p-tau×t-PrP) ratio best distinguished typical AD from atypical CJD with 98.1% sensitivity and 100% specificity.

DISCUSSION {#sec0065}
==========

In this study we investigated for the first time the accuracy of CSF protein t-PrP, t-tau, 14-3-3, p-tau, and Aβ~42~ assays in the differential diagnosis between CJD and AD in a patient series including a high proportion of clinically atypical cases of both diseases. Furthermore, at variance with most previous studies which have mainly focused on t-tau, p-tau, and, to a lesser extent t-PrP, we also considered the added diagnostic value of Aβ~42~.

At variance with classic biomarkers such as t-tau, 14-3-3, p-tau, and Aβ~42,~ there is still limited knowledge about t-PrP in the CSF in both controls and patients with neurodegenerative diseases. To date, few studies have measured CSF PrP levels in CJD, AD, and controls. Our finding of a statistically significant decrease in CSF t-PrP levels in CJD patients is consistent with those of other authors \[[@ref029]\]. Thus, as CSF Aβ~42~ levels inversely correlate with amyloid burden in AD, t-PrP levels likely reflect the extent of PrP^c^ conversion into abnormal PrP (PrP^Sc^) and the burden of PrP^Sc^ deposition in CJD. The latter may also explain our finding of an even higher reduction of t-PrP levels in atypical CJD cases. Indeed, atypical CJD variants such as MV 2K and MM 2C are usually associated with a relatively high amount of PrP^Sc^ accumulation involving major areas of the brain such as the cerebral cortex, basal ganglia, and thalamus.

Our results on t-PrP CSF levels in AD are at variance with both Dorey et al. \[[@ref029]\] and Meyne et al. \[[@ref040]\], who found either increased or decreased t-PrP levels in AD compared to controls. While the latter discrepancy has been attributed to the lack of specificity for the human prion protein of the assay used by Meyne et al. \[[@ref040]\], the former may reflect differences in the selected patient populations. Whatever the case, further studies are needed to clarify the divergence, especially in view of the proposed putative role of PrP^C^ in AD pathogenesis \[[@ref029]\].

Regarding the diagnostic value of t-PrP in the discrimination of CJD from AD, we reported an AUC of 0.825, a sensitivity of 73%, and a specificity of 75% using a cut-off value of 261 ng/ml, which is slightly worse than that reported by Dorey et al. (AUC = 0.886, 82.1% sensitivity and 82.4% specificity) using the same kit and a very similar cut-off value (263 ng/ml). The small discrepancy likely reflects their findings of increased t-PrP levels compared to normal subjects in a subgroup of ADpatients.

In this study, we also addressed the possible diagnostic role of CSF Aβ~42~ and its derived ratios in the clinical distinction between CJD and AD. Van Everbroeck et al. \[[@ref022]\] originally reported that Aβ~42~ levels are decreased in CJD, a finding that has subsequently been confirmed by others \[[@ref022]\]. More recently, however, some studies showed that Aβ~42~ levels and Aβ~42~/p-tau ratio are significantly higher in CJD than in AD patients \[[@ref021]\], thus indicating that the determination of Aβ~42~ levels might contribute to the differential diagnosis between the two diseases. In our CJD population, Aβ~42~ CSF levels were highly heterogeneous, with several cases of both typical and atypical CJD groups showing a lower than cut-off value. Whether this result simply reflects the burden of associated AD pathology, which has to be taken in account considering the mean age of death of CJD patients, or also depends on a pathogenic interaction between the two proteins, as it has recently been suggested \[[@ref042]\], remains to be seen.

Taken together, our results underline the added value of both Aβ~42~ and t-PrP CSF assays in the differential diagnosis between CJD and AD. Indeed, the addition of t-PrP and Aβ~42~ values to those of t-tau and p-tau in a novel ratio (t-tau×Aβ~42~)/(p-tau×t-PrP) distinguishes CJD from AD patients with greater accuracy (0.995 accuracy, 98.1% sensitivity, and 97.7% specificity) than all previously-described ratios such as CJ factor, t-tau/p-tau, and Aβ~42~/p-tau \[[@ref021]\]. Most importantly, the novel ratio also demonstrates superior diagnostic accuracy in the most difficult clinical scenario, which is the clinical distinction between atypical CJD and a/rpAD patients. Indeed, while CSF t-tau and 14-3-3 do not contribute at all to the differential diagnosis of such atypical variants (e.g., 61.1% and 25.9% sensitivity; 50% and 84.1% specificity), in the same situation the novel ratio yields 96.2% sensitivity and 95.5% specificity. We therefore strongly recommend extending the analyses to all four biomarkers in the presence of atypical clinical features and ambiguous results of standard CSF assays. Alternatively, particularly in a laboratory setting where the t-PrP assay is not available, Aβ~42~×t-tau/p-tau and t-tau/p-tau ratios remain the best biomarkers for accuracy, sensitivity, and specificity in distinguishing CJD from AD.

We are aware that one potential limitation of our study is the discrepancy in the number of neuropathologically-verified cases between CJD and AD. Nevertheless, all AD affected subjects included in this study fulfilled the diagnostic criteria of probable AD with high evidence of the pathophysiological process of AD. Furthermore, in all a/rpAD cases, the final diagnosis was formulated at follow-up after at least 2 years of further clinical observation.

In summary, while individually none of the major CSF proteins that reflect the specific molecular pathology of AD (p-tau and Aβ~42~) and CJD (PrP) or the associated neuronal damage (t-tau, 14-3-3) distinguish the two disorders with sufficient accuracy, various combinations of these markers significantly increase the diagnostic power. Among, them the (t-tau×Aβ~42~)/(p-tau×t-PrP) ratio best distinguishes CJD from AD patients and is especially recommended in the diagnostic work-up of patients presenting with atypical clinical features that are compatible with both diseases.

Supplementary Material
======================

###### Supplementary Table

###### 

Click here for additional data file.

The authors thank Italian neurologists for providing clinical information and Barbara Polischi, MSc for her valuable technical assistance.

This work was financially supported by the Italian Ministry of Health, grant RF2011-02351092, and by the Gino Galletti Foundation.

Authors' disclosures available online (<http://j-alz.com/manuscript-disclosures/16-0740r2>).

The supplementary material is available in the electronic version of this article: <http://dx.doi.org/10.3233/JAD-160740>.

![Boxplots illustrate levels of the CSF total prion protein (t-PrP) in control, Alzheimer's disease (AD), and Creutzfeldt-Jakob disease (CJD) populations. Central horizontal lines indicate median values. Boxes illustrate the ranges between lower and upper quartiles. Error bars represent the full ranges of data, excluding outliers, which are displayed separately as single dots.](jad-55-jad160740-g001){#jad-55-jad160740-g001}

![Receiver operating characteristics (ROC) curves illustrate sensitivity and specificity of various cerebrospinal fluid biomarker combinations. Area under the ROC curve is reported as area under the curve (AUC). The corresponding AUC values are also listed in [Table 4](#jad-55-jad160740-t004){ref-type="table"}.](jad-55-jad160740-g002){#jad-55-jad160740-g002}

###### 

Disease duration and histotype classification of CJD cases

                               Typical CJD (N = 54)   Atypical CJD (N = 54)   Total CJD (N = 108)
  --------------------------- ---------------------- ----------------------- ---------------------
  Disease duration (months)           N = 52                 N = 52                 N = 104
    Mean±SD                          5.0±4.0                18.6±14.8              11.8±12.7
    Min-Max                         1.0--20.0               1.0--60.0              1.0--60.0
    Median (IQR)                  4.0 (2.0--6.4)        13.8 (6.5--24.8)        6.3 (3.0--15.5)
  Definite sporadic CJD                 51                     32                     83
    MM1                                 23                      6                     29
    MM1+2C                              8                       5                     13
    VV2                                 15                      0                     15
    MV 2K                               5                      13                     18
    MM 2C                               0                       5                      5
    MM 2T                               0                       2                      2
    VV1                                 0                       1                      1
  Definite genetic CJD                  1                       7                      8
    E200K-129MM(V)                      1                       6                      7
    R208H-129VV                         0                       1                      1
  Probable CJD                          2                      15                     17
    MM                                  0                       3                      3
    MV                                  2                      12                     14

###### 

Clinical and biological features of atypical/rapidly progressive AD

  Time between first compliant     26.3±25.6 (*n* = 44)
  ------------------------------ ------------------------
  Clinical presentation          
    Cognitive decline                     44/44
    Extrapyramidal signs                   9/44
    Pyramidal signs                        3/44
    Myoclonus                              5/44
    Cerebellar signs                       2/44
  Biomarkers Data                
    t-tau \>1200 pg/ml                    22/44
    Positive 14-3-3                        7/44
  Genetic features               
    APOE genotype^§^                  No E4 = 18 (58.1%)
                                    One E4 = 8 (25.8%)
                                     E4/E4 = 5 (16%)
    FAD Mutations^\*^                      2/13
    (APP, PSEN1, PSEN2)              (both in PSEN1)

^§^patients (*n* = 31) with available DNA/informed consent for genetic analyses. ^\*^patients (*n* = 13) with early onset AD (\<65 years) and/or a positive family history.

###### 

CSF biomarker data in the AD and CJD groups

                                      all AD         Typical AD         a/rpAD          all CJD         Typical CJD      Atypical CJD
  ------------------------------ ---------------- ---------------- ---------------- ---------------- ----------------- ----------------
  t-PrP (ng/ml)                                                                                                        
    Median                             335              334              345              173               209              141
    (IQR)                           (234--455)       (281--455)       (224--469)       (103--261)       (131--288)        (83--208)
  t-tau (pg/ml)                                                                                                        
    Median                             822              697              1223             2489             7284              1390
    (IQR)                          (582--1223)       (509--846)      (703--1668)      (1389--7344)     (3022--10004)     (914--2086)
  p-tau (pg/ml)                                                                                                        
    Median                             104               90              122               49               55                46
    (IQR)                           (77--140)        (74--115)        (81--151)         (37--68)         (41--72)          (34--65)
  Aβ~42~ (pg/ml)                                                                                                       
    Median                             358              371              326              527               553              498
    (IQR)                           (266--465)       (279--469)       (250--442)       (366--747)       (353--773)        (370--738)
  14-3-3                                                                                                               
    N° positive                        7/89             0/45             7/44            68/108            54/54            14/54
  t-tau/p-tau                                                                                                          
    Median                             7.67             7.13             9.08             59.3              111              32.2
    (IQR)                          (6.76--10.1)     (6.38--7.98)     (7.35--11.3)     (27.1--110)       (64.5--198)      (20.6--52.1)
  t-tau/t-PrP                                                                                                          
    Median                             2.58             2.22             3.36             17.4             33.6              10.7
    (IQR)                          (1.81--3.86)     (1.68--2.61)     (2.29--5.40)     (8.29--39.4)     (17.4--76.9)      (6.10--17.3)
  Aβ~42~/p-tau                                                                                                         
    Median                             3.63             4.09             3.20             10.1              9.5              12.3
    (IQR)                          (2.47--4.70)     (3.11--5.29)     (1.88--4.23)     (7.18--16.1)     (5.55--14.6)      (8.09--16.7)
  Aβ~42~/ (p-tau×t-PrP)                                                                                                
    Median                            0.010            0.011            0.009            0.062             0.053            0.071
    (IQR)                         (0.007--0.018)   (0.008--0.019)   (0.005--0.016)   (0.035--0.107)   (0.026--0.090)    (0.044--0.144)
  Aβ~42~×t-tau/p-tau                                                                                                   
    Median                             2870             2615             3089            27675             55348            15176
    (IQR)                          (2143--3938)     (2010--3373)     (2344--4661)    (13490--59655)   (30720--121296)   (9099--27419)
  t-tau/(p-tau×t-PrP)                                                                                                  
    Median                            0.024            0.022            0.032            0.370             0.637            0.223
    (IQR)                         (0.018--0.037)   (0.018--0.031)   (0.019--0.045)   (0.161--0.778)   (0.238--1.345)    (0.131--0.460)
  (t-tau×Aβ~42~)/(p-tau×t-PrP)                                                                                         
    Median                             8.63             7.64             10.9             155               263              104
    (IQR)                          (6.02--12.6)     (5.68--10.6)     (6.06--14.8)     (82.8--401)       (134--682)       (69.9--205)

###### 

Comparison of discriminatory power of CSF biomarkers to distinguish AD from CJD

  Biomarker                       Area under   Cut-off value    Patients diagnosed   Sensitivity %   Specificity %  
  ------------------------------ ------------- --------------- -------------------- --------------- --------------- ------
                                                                       CJD                AD                        
  t-PrP                           0.825±0.029  \<261 ng/ml            81/108             24/89           73.0        75.0
  14-3-3                              --       --                     68/108             7/89            63.0        92.1
  Aβ~42~/p-tau                    0.914±0.021  \>5.11                 90/107             15/88           84.1        83.0
  Aβ~42~/(p-tau×t-PrP)            0.956±0.014  \>0.022                94/107             10/88           87.9        88.6
  t-tau                           0.865±0.025  \>1200 pg/ml           87/108             22/89           80.6        75.3
  t-tau/p-tau                     0.982±0.010  \>16.4                103/107             4/88            96.3        95.5
  t-tau/t-PrP                     0.945±0.016  \>5.50                 98/108             8/89            90.7        91.0
  Aβ~42~×t-tau/p-tau              0.984±0.008  \>6677                101/107             5/88            94.4        94.3
  t-tau/(p-tau×t-PrP)             0.984±0.007  \>0.061               104/107             4/88            97.1        95.5
  (t-tau×Aβ~42~)/(p-tau×t-PrP)    0.995±0.004  \>24.0                105/107             2/88            98.1        97.7

###### 

Comparison of discriminatory power of CSF biomarkers in the distinction between atypical AD and atypical CJD

  Biomarker                       Area under the  Cut-off value    Patients diagnosed   Sensitivity %   Specificity %  
  ------------------------------ ---------------- --------------- -------------------- --------------- --------------- ------
                                                                      Atypical CJD         a/rp AD                     
  t-tau                            0.612±0.057    \>1200 pg/ml           33/54              22/44           61.1        50.0
  14-3-3                                --        --                     14/54              7/44            25.9        84.1
  Aβ~42~/p-tau                     0.945±0.025    \>4.70                 48/53              4/44            90.6        90.9
  Aβ~42~/(p-tau×t-PrP)             0.982±0.010    \>0.025                49/53              2/44            92.5        95.5
  t-tau/p-tau                      0.945±0.025    \>16.3                 49/54              4/44            90.6        90.9
  t-tau/t-PrP                      0.852±0.040    \>5.27                 45/54              8/44            83.3        81.8
  Aβ~42~×t-tau/p-tau               0.958±0.020    \>6550                 49/53              3/44            92.5        93.2
  t-tau/(p-tau×t-PrP)              0.955±0.020    \>0.063                50/54              3/44            92.6        93.2
  (t-tau×Aβ~42~)/(p-tau×t-PrP)     0.986±0.010    \>24.0                 51/53              2/44            96.2        95.5
