Absolutely!  It is sad that the writers of the gospels did not include remarks, such as, 'Jesus smiled' or "Jesus and his disciples laughed".  I can't imagine Jesus and the disciples not laughing when Philip tells Nathanael about Jesus.  When Jesus sees Nathanael he stated [John 1:47-50] "Here is truly an Israelite in whom there is no deceit!" Nathanael asked him, "Where did you get to know me?" Jesus answered, "I saw you under the fig tree before Philip called you."  In Israel, going under the fig tree meant that one was going to relieve themselves---since there were no public restrooms---Jesus 'saw' Nathanael under the fig tree answering 'nature's call'.  No wonder, Nathanael stated: "Rabbi, you are the Son of God! You are the King of Israel!

Also, I cannot imagine Jesus with children and not smiling and laughing with them. Or Jesus not smiling at the people he cured---or raising from the dead---the young man of Nain or the young daughter of Jairus---a girl just 12 years old.