tags
sequencelengths
2
166
tokens
sequencelengths
2
166
[ 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
[ "Responses", "to", "these", "EBV", "antigens", "by", "synovial", "lymphocytes", "from", "several", "other", "chronic", "rheumatoid", "arthritis", "patients", "were", "readily", "detectable", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
[ "Thus", "these", "results", "suggest", "a", "direct", "contribution", "of", "EBV", "to", "chronic", "rheumatoid", "arthritis", "pathogenesis", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
[ "They", "also", "demonstrate", "for", "the", "first", "time", "the", "occurrence", "of", "T", "cell", "responses", "against", "EBV", "transactivating", "factors", ",", "which", "might", "be", "central", "in", "the", "control", "of", "virus", "reactivation", "." ]
[ 3, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0 ]
[ "CD14", "-mediated", "signal", "pathway", "of", "Porphyromonas", "gingivalis", "lipopolysaccharide", "in", "human", "gingival", "fibroblasts", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 5, 0, 3, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
[ "Lipopolysaccharide", "(", "LPS", ")", "induces", "expression", "of", "inflammatory", "cytokines", "in", "monocytes/macrophages", "via", "CD14", ",", "one", "of", "the", "LPS", "receptors", ",", "which", "is", "expressed", "predominantly", "in", "these", "cells", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 5, 6, 6, 0 ]
[ "It", "has", "been", "demonstrated", "that", "Porphyromonas", "gingivalis", "LPS", "(", "P-LPS", ")", "also", "is", "able", "to", "induce", "inflammatory", "cytokines", "in", "human", "gingival", "fibroblasts", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0 ]
[ "Therefore", ",", "it", "is", "important", "to", "determine", "whether", "CD14", "is", "expressed", "in", "gingival", "fibroblasts", "and", "to", "define", "the", "P-LPS-mediated", "signal-transducing", "mechanism", "in", "the", "cells", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0 ]
[ "In", "this", "study", ",", "we", "observed", "unexpectedly", "by", "immunohistochemical", ",", "Western", "blotting", "(", "immunoblotting", ")", ",", "and", "Northern", "(", "RNA", ")", "blotting", "assays", "that", "CD14", "is", "expressed", "at", "high", "density", "in", "human", "gingival", "fibroblasts", "." ]
[ 0, 0, 0, 0, 1, 2, 2, 2, 2, 2, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0 ]
[ "P-LPS-induced", "expression", "of", "the", "monocyte", "chemoattractant", "protein", "1", "(", "MCP-1", ")", "gene", "in", "the", "cells", "was", "inhibited", "markedly", "by", "treatment", "with", "anti-human", "CD14", "antibody", "and", "was", "completely", "inhibited", "by", "herbimycin", "A", ",", "a", "potent", "inhibitor", "of", "tyrosine", "kinase", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0 ]
[ "The", "inhibitor", "also", "dramatically", "inhibited", "monocyte", "chemotactic", "activity", "of", "and", "MCP-1", "production", "by", "the", "cells", "." ]
[ 0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0 ]
[ "Furthermore", ",", "P-LPS-induced", "expression", "of", "the", "MCP-1", "gene", "in", "the", "cells", "also", "was", "blocked", "by", "inhibitors", "of", "two", "transcription", "factors", ",", "i.e.", ",", "curcumin", ",", "an", "inhibitor", "of", "AP-1", ",", "and", "pyrolidine", "dithiocarbamate", ",", "an", "inhibitor", "of", "NF-kappaB", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
[ "Both", "inhibitors", "inhibited", "monocyte", "chemotactic", "activity", "in", "the", "culture", "supernatant", "of", "P-LPS-treated", "cells", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 3, 0, 0, 0, 0, 0 ]
[ "Gel", "shift", "mobility", "assay", "showed", "stimulation", "of", "the", "AP-1", "and", "NF-kappaB", "contents", "in", "P-LPS-treated", "cells", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 5, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0 ]
[ "This", "study", "is", "the", "first", "to", "demonstrate", "the", "expression", "of", "CD14", "in", "human", "gingival", "fibroblasts", "and", "to", "show", "that", "the", "signal-transducing", "pathway", "of", "P-LPS", "in", "the", "cells", "is", "mediated", "by", "CD14", "." ]
[ 3, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0 ]
[ "rel", "Is", "rapidly", "tyrosine-phosphorylated", "following", "granulocyte-colony", "stimulating", "factor", "treatment", "of", "human", "neutrophils", "." ]
[ 0, 0, 5, 0, 3, 4, 4, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
[ "Stimulation", "of", "neutrophils", "with", "granulocyte-colony", "stimulating", "factor", "(", "G-CSF", ")", "results", "in", "an", "enhanced", "respiratory", "burst", ",", "prolonged", "survival", ",", "and", "increased", "tumor", "cell", "killing", "." ]
[ 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0 ]
[ "The", "effects", "of", "G-CSF", "are", "mediated", "by", "binding", "to", "specific", ",", "high", "affinity", "receptors", "." ]
[ 3, 4, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
[ "G-CSF", "receptors", "lack", "intrinsic", "tyrosine", "kinase", "activity", ",", "but", "activation", "of", "the", "receptor", "results", "in", "the", "rapid", "induction", "of", "tyrosine", "kinase", "activity", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 5, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
[ "Antiphosphotyrosine", "immunoblots", "of", "whole", "cell", "lysates", "prepared", "from", "neutrophils", "show", "that", "the", "G-CSF", "rapidly", "induces", "prominent", "tyrosine", "phosphorylation", "of", "a", "protein", "of", "a", "relative", "molecular", "mass", "of", "80", "kDa", "." ]
[ 0, 3, 4, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 3, 0, 0, 1, 0, 0, 0, 0, 0, 3, 4, 0, 0, 3, 0 ]
[ "Using", "monospecific", "antibodies", ",", "the", "80-kDa", "tyrosine-phosphorylated", "protein", "has", "been", "shown", "to", "be", "p80c-rel", ",", "a", "proto-oncogene", "belonging", "to", "a", "family", "of", "transcriptional", "regulators", "which", "include", "NF-kB", "." ]
[ 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 3, 0, 0, 3, 4, 4, 4, 0, 0, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0 ]
[ "The", "induction", "of", "tyrosine", "phosphorylation", "of", "p80c-rel", "was", "unique", "to", "G-CSF", "in", "that", "granulocyte-macrophage", "colony", "stimulating", "factor", "which", "also", "stimulates", "neutrophils", "and", "induces", "tyrosine", "phosphorylation", "does", "not", "result", "in", "tyrosine", "phosphorylation", "of", "p80c-rel", "." ]
[ 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0 ]
[ "The", "consequences", "of", "p80c-rel", "tyrosine", "phosphorylation", "are", "not", "yet", "known", ";", "however", ",", "tyrosine-phosphorylated", "p80c-rel", "is", "capable", "of", "binding", "to", "DNA", ",", "and", "G-CSF", "stimulation", "results", "in", "an", "increase", "in", "the", "amount", "of", "p80c-rel", "which", "binds", "to", "DNA", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 0, 3, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0 ]
[ "These", "results", "demonstrate", "that", "one", "of", "the", "first", "biochemical", "events", "which", "occurs", "in", "neutrophils", "following", "G-CSF", "stimulation", ",", "activation", "of", "a", "tyrosine", "kinase", ",", "leads", "directly", "to", "the", "tyrosine", "phosphorylation", "of", "p80c-rel", "." ]
[ 0, 0, 0, 3, 4, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0 ]
[ "Thus", ",", "the", "tyrosine", "kinase", "activated", "by", "G-CSF", "appears", "to", "directly", "transduce", "a", "signal", "to", "a", "protein", "which", "functions", "as", "a", "transcriptional", "regulator", "." ]
[ 0, 0, 3, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0 ]
[ "Patterns", "of", "Pan", "expression", "and", "role", "of", "Pan", "proteins", "in", "endocrine", "cell", "type-specific", "complex", "formation", "." ]
[ 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 3, 0, 3, 0, 0, 0, 0, 3, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
[ "The", "Pan", "gene", "encodes", "at", "least", "two", "distinct", "transcripts", ",", "Pan-1", "and", "Pan-2", "(", "also", "known", "as", "E47", "and", "E12", ",", "respectively", ")", ",", "by", "the", "mechanism", "of", "alternative", "RNA", "splicing", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0 ]
[ "Northern", "blot", "analyses", "performed", "on", "rat", "and", "mouse", "tissues", "have", "detected", "ubiquitously", "expressed", "Pan", "transcripts", ",", "but", "the", "abundance", ",", "distribution", ",", "and", "form", "of", "Pan", "proteins", "have", "not", "been", "clearly", "defined", "." ]
[ 0, 0, 7, 8, 0, 5, 6, 6, 6, 6, 6, 6, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 5, 0 ]
[ "Studies", "of", "cell", "lines", "representing", "endocrine", ",", "fibroblast", ",", "and", "lymphoid", "lineages", "using", "polyclonal", "antisera", "to", "detect", "E2A", "proteins", "have", "suggested", "that", "significant", "E2A", "protein", "expression", "is", "restricted", "to", "B-lymphocytes", "." ]
[ 0, 0, 0, 0, 3, 4, 0, 3, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 5, 6, 6, 6, 6, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
[ "We", "have", "developed", "a", "monoclonal", "antibody", ",", "Yae", ",", "which", "is", "specific", "for", "Pan/E2A", "proteins", ",", "and", "have", "used", "the", "Yae", "antibody", "to", "examine", "a", "variety", "of", "endocrine", "and", "nonendocrine", "cell", "lineages", "for", "differences", "in", "Pan/", "E2A", "protein", "expression", ",", "subcellular", "localization", ",", "and", "heteromeric", "complex", "formation", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 3, 4, 0, 7, 8, 8, 8, 8, 8, 8, 8, 0 ]
[ "In", "contrast", "to", "previous", "results", "obtained", "using", "polyclonal", "antiseras", "to", "detect", "Pan/", "E2A", "proteins", ",", "we", "report", "comparable", "levels", "of", "Pan", "proteins", "in", "GH/PRL-", "and", "insulin-producing", ",", "B-", "and", "T-lymphocyte", "cells", "." ]
[ 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0 ]
[ "IEF-1", ",", "a", "pancreatic", "beta-cell", "type-specific", "complex", "believed", "to", "regulate", "insulin", "expression", ",", "is", "demonstrated", "to", "consist", "of", "at", "least", "two", "distinct", "species", ",", "one", "of", "which", "does", "not", "contain", "Pan", "molecules", "." ]
[ 0, 0, 0, 0, 0, 0, 5, 6, 6, 0, 5, 6, 6, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 5, 6, 0, 3, 4, 0, 0, 3, 4, 4, 0 ]
[ "Although", "it", "has", "been", "postulated", "that", "pituitary", "endocrine", "cells", "and", "pancreatic", "endocrine", "beta-cells", "share", "identical", "Pan/E2A", "complexes", ",", "native-Western", "analyses", "of", "pituitary", "and", "endocrine", "beta-cells", "detect", "Pan", "proteins", "in", "distinct", "cell", "type-specific", "complexes", "." ]
[ 0, 0, 0, 3, 4, 4, 4, 4, 0, 0, 0, 0, 5, 6, 6, 0, 0, 0, 0 ]
[ "Visualization", "of", "the", "endogenous", "NF-kappa", "B", "p50", "subunit", "in", "the", "nucleus", "of", "follicular", "dendritic", "cells", "in", "germinal", "centers", "." ]
[ 3, 4, 0, 0, 3, 4, 4, 4, 4, 4, 4, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0 ]
[ "NF-kappa", "B", ",", "a", "50", "kDa/65", "kDa", "(", "p50/p65", ")", "heterodimer", ",", "is", "a", "ubiquitous", "transcription", "factor", "involved", "in", "the", "positive", "regulation", "of", "various", "immune", "genes", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
[ "The", "aim", "of", "this", "study", "was", "to", "determine", "whether", "NF-kappa", "B", "is", "related", "to", "a", "particular", "cell", "type", "and/or", "differentiation", "step", "during", "immunopoiesis", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0, 5, 0, 0 ]
[ "Using", "in", "situ", "hybridization", "on", "sections", "from", "non", "HIV", "hyperplastic", "lymph", "nodes", ",", "we", "found", "that", "the", "gene", "of", "the", "105", "kDa", "precursor", "of", "p50", "was", "overexpressed", "in", "the", "light", "zone", "of", "germinal", "centers", ",", "with", "a", "network", "aspect", ",", "which", "suggested", "the", "involvement", "of", "follicular", "dendritic", "cells", "(", "FDC", ")", "." ]
[ 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 0, 0, 0, 0, 0, 5, 0 ]
[ "By", "immunohistochemistry", ",", "p50", "protein", "was", "detected", "in", "the", "cytoplasm", "and", "nucleus", "of", "FDC", ",", "confirming", "the", "involvement", "of", "FDC", "." ]
[ 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 5, 0 ]
[ "Furthermore", ",", "p50", "protein", "was", "detected", "in", "the", "cytoplasm", "of", "all", "lymphocytes", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 5, 0, 0, 0, 0, 0 ]
[ "Thus", ",", "we", "focused", "our", "study", "on", "isolated", "FDC", "clusters", "from", "normal", "tonsils", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 5, 0 ]
[ "As", "showed", "on", "tissue", "sections", ",", "we", "detected", "the", "p50", "in", "both", "cytoplasm", "and", "nucleus", "of", "FDC", "." ]
[ 0, 0, 5, 0, 5, 0, 0, 0, 0 ]
[ "Nuclei", "of", "lymphocytes", "from", "FDC", "clusters", "were", "negative", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 0, 0 ]
[ "We", "next", "studied", "p65", "and", "c-Rel", "protein", "expression", "in", "FDC", "clusters", "." ]
[ 3, 0, 0, 0, 0, 0, 0, 5, 0, 0, 0, 0, 0, 0 ]
[ "p65", "was", "detected", "in", "the", "cytoplasm", "of", "FDC", ",", "whereas", "nuclei", "were", "negative", "." ]
[ 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 5, 0 ]
[ "Furthermore", ",", "p65", "was", "detected", "in", "the", "nuclei", "of", "some", "lymphocytes", "." ]
[ 3, 4, 0, 0, 0, 0, 0, 0, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 5, 0 ]
[ "c-Rel", "protein", "was", "detected", "only", "in", "the", "cytoplasm", "of", "lymphocytes", "and", "not", "in", "the", "nucleus", "and", "cytoplasm", "of", "FDC", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 0, 0, 3, 0, 0, 0, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 5, 0, 5, 0, 0 ]
[ "Our", "results", "indicated", "that", ",", "in", "the", "context", "of", "T", "cell-dependent", "B", "cell", "immunopoiesis", "occurring", "in", "FDC", "clusters", ",", "p50", "is", "mainly", "related", "to", "FDC", "with", "a", "massive", "overexpression", "in", "the", "nuclei", ",", "whereas", "p65", "is", "expressed", "in", "a", "scattered", "manner", "in", "the", "nuclei", "of", "lymphocytes", "and", "c-Rel", "protein", "exclusively", "in", "the", "cytoplasm", "of", "lymphocytes", "from", "FDC", "clusters", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
[ "These", "results", "suggested", "that", "the", "two", "subunits", "of", "NF-kappa", "B", "and", "the", "c-Rel", "protein", "have", "different", "roles", "in", "different", "cell", "types", "during", "B", "cell", "immunopoiesis", "." ]
[ 0, 0, 0, 0, 3, 0, 3, 0, 0, 0, 0, 0, 3, 0 ]
[ "Cloning", "and", "characterization", "of", "NF-ATc", "and", "NF-ATp", ":", "the", "cytoplasmic", "components", "of", "NF-AT", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0 ]
[ "Present", "evidence", "indicates", "a", "pathway", "of", "signal", "transmission", "in", "T", "cells", "that", "is", "outlined", "in", "figure", "1", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 3, 4, 3, 0 ]
[ "The", "elevation", "in", "intracellular", "calcium", "that", "is", "induced", "by", "interactions", "at", "the", "antigen", "receptor", "leads", "to", "the", "activation", "of", "the", "calcium-dependent", "phosphatase", "calcineurin", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 3, 0 ]
[ "This", "in", "turn", "leads", "to", "the", "nuclear", "association", "of", "the", "cytosolic", "component", "of", "NF-ATc", "." ]
[ 0, 0, 0, 3, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0 ]
[ "The", "activation", "of", "calcineurin", "and", "the", "nuclear", "import", "of", "NF-ATc", "can", "both", "be", "blocked", "by", "cyclosporin", "A", "or", "FK506", "in", "complex", "with", "their", "respective", "immunophilins", "." ]
[ 0, 0, 0, 0, 0, 3, 0, 0, 3, 0, 0, 0, 3, 4, 4, 0 ]
[ "Once", "in", "the", "nucleus", ",", "NF-ATc", "interacts", "with", "NF-ATn", "to", "form", "an", "active", "transcriptional", "complex", "." ]
[ 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0 ]
[ "NF-ATn", "is", "a", "ubiquitous", "protein", ",", "can", "be", "synthesized", "in", "response", "to", "PMA", ",", "and", "has", "many", "similarities", "to", "AP-1", "." ]
[ 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0 ]
[ "The", "mechanism", "by", "which", "NF-ATc", "enters", "the", "nucleus", "is", "unknown", ",", "and", "although", "it", "appears", "to", "require", "calcineurin", ",", "NF-ATc", "has", "not", "yet", "been", "shown", "to", "be", "an", "in", "vivo", "substrate", "of", "calcineurin", "." ]
[ 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 3, 0 ]
[ "Alternative", "mechanisms", "include", "the", "possibility", "that", "NF-ATc", "operates", "on", "some", "cytoplasmic", "anchor", "or", "that", "other", "proteins", "that", "are", "controlled", "by", "calcineurin", "carry", "out", "the", "nuclear", "import", "of", "NF-ATc", "." ]
[ 0, 3, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0 ]
[ "Although", "NF-ATp", "copurifies", "with", "NF-ATc", ",", "there", "is", "as", "yet", "no", "understanding", "of", "how", "NF-ATp", "is", "functioning", "in", "vivo", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
[ "Now", "that", "these", "proteins", "are", "purified", "and", "cloned", ",", "the", "major", "goals", "will", "be", "to", "understand", "their", "role", "and", "the", "roles", "of", "other", "family", "members", "in", "thymic", "development", "." ]
[ 0, 0, 3, 4, 4, 4, 0, 3, 4, 0 ]
[ "Induction", "of", "vascular", "cell", "adhesion", "molecule-1", "by", "low-density", "lipoprotein", "." ]
[ 3, 4, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
[ "Low-density", "lipoprotein", "(", "LDL", ")", "is", "a", "well-established", "risk", "factor", "for", "atherosclerosis", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
[ "When", "endothelial", "cells", "are", "incubated", "with", "this", "lipoprotein", "in", "pathophysiologic", "amounts", ",", "the", "cells", "are", "activated", "." ]
[ 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
[ "Among", "the", "documented", "cellular", "responses", "to", "LDL", "is", "increased", "recruitment", "of", "monocytes", ",", "which", "are", "believed", "to", "play", "a", "major", "role", "in", "promoting", "intimal", "plaque", "formation", "." ]
[ 0, 0, 0, 0, 0, 0, 3, 4, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 3, 4, 4, 4, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0 ]
[ "The", "findings", "presented", "here", "link", "an", "atheogenic", "lipoprotein", ",", "LDL", ",", "with", "the", "induction", "of", "an", "adhesion", "molecule", "important", "in", "atherogenesis", "Human", "LDL", "induces", "the", "vascular", "cell", "adhesion", "molecule-1", "(", "VCAM-1", ")", "transcriptionally", "with", "an", "increase", "in", "mRNA", "levels", "through", "activation", "of", "the", "VCAM", "promoter", "." ]
[ 0, 0, 0, 0, 0, 3, 4, 0 ]
[ "This", "effect", "is", "blocked", "by", "anti-VCAM", "antibodies", "." ]
[ 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0 ]
[ "After", "a", "2-day", "incubation", "in", "LDL", ",", "the", "binding", "of", "NF-kappa", "B", ",", "which", "is", "believed", "to", "be", "a", "key", "oxidative-stress", "sensor", "for", "VCAM", "regulation", ",", "remains", "at", "basal", "level", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 3, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0 ]
[ "In", "contrast", ",", "the", "binding", "activities", "of", "AP-1", "and", "GATA", ",", "on", "the", "other", "hand", ",", "are", "increased", "by", "LDL", "." ]
[ 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 5, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 3, 0 ]
[ "Thus", ",", "a", "component", "of", "LDL", "-enhanced", "endothelial", "recruitment", "of", "monocytes", "is", "attributed", "to", "VCAM-1", "expression", ",", "which", "appears", "to", "be", "mediated", "through", "AP-1", "and", "GATA", "." ]
[ 0, 0, 0, 3, 0, 0, 3, 0, 0, 0, 0, 3, 0, 3, 0 ]
[ "These", "data", "identify", "LDL", "as", "a", "VCAM", "-inducer", "possibly", "distinct", "from", "cytokines", "and", "endotoxin", "." ]
[ 0, 0, 3, 4, 4, 0, 0, 3, 0, 0, 5, 6, 6, 0 ]
[ "Involvement", "of", "nuclear", "factor-kappa", "B", "activation", "in", "IgE", "synthesis", "in", "human", "B", "cells", "." ]
[ 3, 4, 4, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 0, 0, 1, 2, 0, 1, 2, 0 ]
[ "Nuclear", "factor-kappa", "B", "(", "NF-kappa", "B", ")", "is", "a", "transcription", "factor", "that", "binds", "to", "the", "consensus", "DNA", "sequence", "in", "the", "cis-acting", "elements", "of", "various", "genes", "." ]
[ 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0 ]
[ "Although", "NF-kappa", "B", "activates", "the", "expression", "of", "many", "genes", "involved", "in", "immune", "and", "inflammatory", "responses", ",", "little", "is", "known", "about", "the", "role", "of", "NF-kappa", "B", "activation", "in", "the", "induction", "of", "IgE", "synthesis", "in", "human", "B", "cells", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 7, 8, 8, 0, 0, 0, 7, 8, 8, 8, 8, 8, 0, 7, 0 ]
[ "Therefore", "we", "first", "examined", "the", "participation", "of", "NF-kappa", "B", "in", "germline", "C", "epsilon", "transcription", "in", "a", "human", "Burkitt", "lymphoma", "B", "cell", "line", ",", "DND39", "." ]
[ 0, 0, 7, 8, 0, 3, 0, 3, 4, 4, 0, 3, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
[ "Stimulation", "of", "DND39", "cells", "with", "IL-4", "or", "anti-CD40", "monoclonal", "antibody", "(", "mAb", ")", "activated", "phosphatidylinositol", "3-kinase", "and", "subsequently", "induced", "nuclear", "expression", "of", "NF-kappa", "B", ",", "which", "was", "identified", "by", "electrophoretic", "mobility", "shift", "assays", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 3, 0, 0, 3, 4, 0 ]
[ "n-Acetyl-L-cysteine", "(", "NAC", ")", ",", "a", "potent", "antioxidant", ",", "blocked", "NF-kappa", "B", "activation", "caused", "by", "IL-4", "and", "by", "anti-CD40", "mAb", "." ]
[ 0, 0, 0, 7, 8, 8, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 1, 0, 0, 0, 0 ]
[ "Although", "inhibition", "of", "IL-4-driven", "germline", "C", "epsilon", "transcription", "by", "NAC", "was", "not", "sufficient", ",", "the", "agent", "remarkably", "diminished", "anti-CD40", "mAb", "-mediated", "up-regulation", "of", "germline", "C", "epsilon", "transcription", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 3, 4, 0 ]
[ "Second", ",", "we", "studied", "the", "effect", "of", "NAC", "on", "IgE", "synthesis", "in", "human", "normal", "B", "cells", "costimulated", "with", "IL-4", "and", "anti-CD40", "mAb", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0 ]
[ "NAC", "was", "effective", "in", "inhibiting", "mature", "C", "epsilon", "transcription", "and", "IgE", "synthesis", "in", "the", "T", "cell-independent", "culture", "system", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 5, 6, 6, 0 ]
[ "However", ",", "NAC", "did", "not", "significantly", "affect", "the", "spontaneous", "production", "of", "IgE", "by", "atopic", "B", "cells", "." ]
[ 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 7, 8, 0, 3, 0, 3, 4, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 5, 6, 0 ]
[ "These", "results", "indicate", "that", "NF-kappa", "B", "activity", "is", "commonly", "inducible", "in", "DND39", "cells", "by", "IL-4", "and", "anti-CD40", "mAb", "and", "suggest", "that", "NF-kappa", "B", "sensitive", "to", "NAC", "may", "play", "a", "role", "in", "regulating", "IgE", "synthesis", "in", "B", "cells", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 1, 0, 1, 2, 0, 5, 6, 6, 0 ]
[ "Prostaglandin", "E2", "induction", "of", "binding", "activity", "to", "CRE", "and", "AP-2", "elements", "in", "human", "T", "lymphocytes", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
[ "Prostaglandins", "of", "the", "E", "series", "are", "immunomodulatory", "agents", "which", "exert", "inhibitory", "as", "well", "as", "stimulatory", "effects", "on", "a", "variety", "of", "immune", "responses", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0 ]
[ "Since", "it", "is", "known", "that", "PGE2", "is", "able", "to", "increase", "cAMP", "levels", ",", "we", "investigated", "whether", "it", "can", "affect", "gene", "expression", "through", "the", "activation", "of", "the", "transcription", "factors", "which", "bind", "enhancer", "elements", "in", "the", "promoter", "regions", "of", "cAMP-regulated", "genes", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0, 0, 0, 0, 0, 0, 0, 1, 0, 1, 0, 0, 0, 1, 2, 2, 2, 0 ]
[ "Using", "electrophoretic", "mobility", "shift", "assay", ",", "we", "demonstrated", "that", "a", "short", "treatment", "of", "human", "T", "lymphocytes", "with", "PGE2", "induces", "specific", "binding", "activity", "to", "CRE", "and", "AP-2", ",", "but", "not", "AP-1", ",", "DNA", "elements", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
[ "Since", "the", "okadaic", "acid", ",", "a", "potent", "protein", "phosphatase", "inhibitor", ",", "prolongs", "the", "induction", "of", "the", "binding", "activity", ",", "phosphorylation", "events", "are", "likely", "to", "occur", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
[ "This", "activity", "seems", "to", "be", "due", "to", "increased", "cAMP", "levels", "because", "forskolin", "and", "IBMX", "mimic", "the", "effects", "of", "PGE2", "." ]
[ 0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0 ]
[ "More", "interestingly", ",", "transfection", "experiments", "with", "CRE-CAT", "plasmide", "show", "that", "PGE2", "activates", "the", "transcription", "of", "a", "CRE-containing", "promoter", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
[ "These", "data", "support", "the", "positive", "role", "for", "PGE2", "on", "some", "immune", "functions", "." ]
[ 5, 6, 6, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
[ "Nasal", "NK-", "and", "T-cell", "lymphomas", "share", "the", "same", "type", "of", "Epstein-Barr", "virus", "latency", "as", "nasopharyngeal", "carcinoma", "and", "Hodgkin", "'s", "disease", "." ]
[ 5, 6, 6, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 6, 6, 6, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 2, 2, 2, 0, 0, 0, 0, 0, 0 ]
[ "Nasal", "T/NK-cell", "lymphomas", "can", "be", "further", "separated", "into", "those", "of", "natural", "killer", "(", "NK", ")", "cell", "lineage", "or", "of", "T-cell", "lineage", ",", "with", "differences", "in", "cellular", "phenotype", ",", "T-cell", "receptor", "(", "TcR", ")", "gene", "rearrangement", "and", "TcR", "transcript", "expression", "." ]
[ 0, 5, 6, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
[ "Both", "NK-", "and", "T-cell", "subtypes", "are", "closely", "associated", "with", "Epstein-Barr", "virus", "(", "EBV", ")", "." ]
[ 0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
[ "In", "this", "study", ",", "EBV", "gene", "expression", "was", "determined", "in", "23", "cases", "of", "nasal", "lymphoma", "(", "NL", ")", "by", "in", "situ", "hybridisation", "(", "ISH", ")", ",", "reverse", "transcriptase", "-polymerase", "chain", "reaction", "(", "RT-PCR", ")", "and", "immunohistochemistry", "(", "IH", ")", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
[ "Of", "the", "23", "cases", ",", "19", "were", "classified", "as", "NK-cell", "and", "4", "as", "T-cell", "tumours", "." ]
[ 0, 0, 9, 10, 10, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0 ]
[ "ISH", "for", "EBV-encoded", "small", "non-polyadenylated", "RNAs", "showed", "that", "all", "cases", ",", "whether", "NK", "or", "T", ",", "harboured", "EBV", "in", "virtually", "all", "tumour", "cells", "." ]
[ 0, 0, 0, 5, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 3, 4, 4, 0, 0, 9, 10, 10, 10, 0, 0, 0, 0, 9, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
[ "RT-PCR", "demonstrated", "that", "NL", "of", "both", "subtypes", "expressed", "EBNAI", "of", "the", "QUK", "splice", "pattern", ",", "the", "latent", "membrane", "proteins", ",", "LMP1", "and", "2", "and", "the", "BamHI", "A", "rightward", "transcripts", "in", "the", "absence", "of", "EBNA2", "mRNAs", ",", "compatible", "with", "the", "latency", "type", "II", "pattern", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
[ "In", "addition", ",", "analysis", "of", "EBV", "protein", "expression", "by", "IH", "revealed", "a", "heterogeneous", "pattern", "of", "EBV", "gene", "expression", "at", "the", "single-cell", "level", "consisting", "of", "both", "LMP1+", "and", "LMP1-", "tumour", "cells", ",", "suggesting", "a", "mixture", "of", "latency", "I", "and", "II", "." ]
[ 0, 0, 3, 4, 4, 0, 3, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
[ "Although", "2", "early", "lytic", "transcripts", ",", "BZLF1", "and", "BHRF1", ",", "were", "also", "detected", "in", "13", "and", "10", "cases", ",", "respectively", ",", "the", "lack", "of", "ZEBRA", "staining", "in", "any", "case", "indicates", "that", "these", "lytic", "transcripts", "are", "most", "likely", "expressed", "by", "rare", "cells", "in", "the", "biopsies", "entering", "lytic", "cycle", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
[ "The", "viral", "transcriptional", "pattern", "similar", "to", "that", "of", "nasopharyngeal", "carcinoma", "and", "Hodgkin", "'s", "disease", "suggests", "that", "EBV", "can", "exploit", "common", "regulatory", "mechanisms", "for", "gene", "transcription", "in", "diverse", "host", "cell", "types", "." ]
[ 0, 0, 3, 4, 0, 3, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0 ]
[ "Down-regulation", "of", "immunogenic", "proteins", "(", "EBNA2-EBNA6", ")", "in", "nasal", "lymphoma", "may", "enable", "tumour", "cells", "to", "evade", "host", "cytotoxic", "T-cell", "surveillance", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
[ "Potent", "gene", "regulatory", "and", "antiproliferative", "activities", "of", "20-methyl", "analogues", "of", "1", ",", "25", "dihydroxyvitamin", "D3", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
[ "The", "biological", "active", "form", "of", "vitamin", "D3", ",", "1", ",", "25-dihydroxyvitamin", "D3", "(", "VD", ")", ",", "regulates", "cellular", "growth", "and", "differentiation", "." ]