What do restriction endonucleases do?
Restiction Endonucleases (RE) : In order to transfer a gene, it is to be first selectively split from the parent DNA. It is usually achieved by the restriction endonucleases. RE have specific recognition sites where they cut the DNA. There are >800 such enzymes now available commercially. These enzyms recognize specific sequence with palindrome arrangement. REF :DM VASUDEVAN textbook; 8th EDITION; Page no :601
Metabolism of nucleic acids
Cut ds DNA at specific sites
Cut RNA at specific sites
Cut ss DNA at specific sites
Break peptide chains
Cut ds DNA at specific sites