Opacity around colonies of Clostridium perfringens is due to -
Opacity around colonies of Clostridium perfringens is due to lecithinase. Option 1 Theta-toxin- hemolysin & cytolytic toxin. Option 2 Nagler reaction: Shown by C. perfringens as opalescence on serum or egg yolk media due to Lecithinase C activity of alpha toxin, it splits lecithin into phosphorylcholine and diglyceride in the presence of Ca2+ and Mg2+ ions.(Refer to the image below) It is a neutralisation reaction. Option 3, 4 - Desmolase: It is not produced by Clostridium perfringens. - Cytokinins (CK) are a class of plant growth substances (phytohormones) that promote cell division, or cytokinesis, in plant roots and shoots.
Systemic Bacteriology (Gram Positive Bacilli, Gram Negative Bacilli)
Theta toxin
Lecithinase
Desmolase
Cytokinin
Lecithinase