Which specimen shouldn't be refrigerated before primary innoculation?
Ref: Ananthnarayan R. Paniker CKJ. Textbook of Microbiology. 8th Edition. Hyderabad: Universities Press; 2009. Pg. 332Explanation:Haemophilus influenzae is an important cause of meningitis.The disease is more common in children between two months and three years of age. Infections are caused by the capsulated strains, type b accounting for most cases.CSF specimen from suspected children should be plated promptly on a suitable medium such as blood agar or chocolate agar and incubated in an environment of 5-10 per cent C()2 and high humidity.As this bacillus is very sensitive to low temperatures. CSF specimens should never be refrigerated before inoculation.
Culture media and method
CSF
Urine
Sputum
Pus
CSF