A farmer presenting with fever off and on for the past 4 years was diagnosed to be suffering from chronic Brucellosis. All of the following serological tests would be helpful in the diagnosis at this state except:
Option 1, 2, 4 - Acute infection: Standard agglutination test (SAT) - This test detects mainly IgM antibodies which are absent in chronic brucellosis. - SAT performed with 2-mercaptoethanol treated with serum will detects only IgG - Blocking antibodies--IgA antibodies that interfere with agglutination by IgG and IgM. They appear during the subacute stage of infection, tend to persist for many years- detected by the Coombs test Option 3 - CFT and ELISA: Are preferred in chronic brucellosis for Ab detection. Other Tests: PCR using primers for rrs-rrl gene, Omp2 gene Brucellin skin test Diagnosis of Brucellosis in Animals: Antibody detection in milk by (i) Milk ring test,(ii) Rose Bengal card test and (iii) Whey agglutination test
Systemic Bacteriology (Haemophilus, Yersinia, Spirochaetes, Ricketssia, Chlamydia, Mycoplasma and Miscellaneous Bacteria)
Standard agglutination test
2-mercapto-ethanol test
Complement fixation test
Coomb's test
Standard agglutination test