TY - JOUR T1 - DNA Origami with Complex Curvatures in Three-Dimensional Space JF - Science JO - Science SP - 342 LP - 346 DO - 10.1126/science.1202998 VL - 332 IS - 6027 AU - Han, Dongran AU - Pal, Suchetan AU - Nangreave, Jeanette AU - Deng, Zhengtao AU - Liu, Yan AU - Yan, Hao Y1 - 2011/04/15 UR - http://science.sciencemag.org/content/332/6027/342.abstract N2 - We present a strategy to design and construct self-assembling DNA nanostructures that define intricate curved surfaces in three-dimensional (3D) space using the DNA origami folding technique. Double-helical DNA is bent to follow the rounded contours of the target object, and potential strand crossovers are subsequently identified. Concentric rings of DNA are used to generate in-plane curvature, constrained to 2D by rationally designed geometries and crossover networks. Out-of-plane curvature is introduced by adjusting the particular position and pattern of crossovers between adjacent DNA double helices, whose conformation often deviates from the natural, B-form twist density. A series of DNA nanostructures with high curvature—such as 2D arrangements of concentric rings and 3D spherical shells, ellipsoidal shells, and a nanoflask—were assembled. ER -