TY - JOUR T1 - Hepatitis C virus glycoproteins mediate pH-dependent cell entry of pseudotyped retroviral particles JF - Proceedings of the National Academy of Sciences JO - Proc Natl Acad Sci USA SP - 7271 LP - 7276 DO - 10.1073/pnas.0832180100 VL - 100 IS - 12 AU - Hsu, Mayla AU - Zhang, Jie AU - Flint, Mike AU - Logvinoff, Carine AU - Cheng-Mayer, Cecilia AU - Rice, Charles M. AU - McKeating, Jane A. Y1 - 2003/06/10 UR - http://www.pnas.org/content/100/12/7271.abstract N2 - HIV pseudotypes bearing native hepatitis C virus (HCV) glycoproteins (strain H and Con1) are infectious for the human hepatoma cell lines Huh-7 and PLC/PR5. Infectivity depends on coexpression of both E1 and E2 glycoproteins, is pH-dependent, and can be neutralized by mAbs mapping to amino acids 412–447 within E2. Cell-surface expression of one or all of the candidate receptor molecules (CD81, low-density lipoprotein receptor, scavenger receptor class B type 1, and dendritic cell-specific intercellular adhesion molecule 3 grabbing nonintegrin) failed to confer permissivity to HIV–HCV pseudotype infection. However, HIV–HCV pseudotype infectivity was inhibited by a recombinant soluble form of CD81 and a mAb specific for CD81, suggesting that CD81 may be a component of a receptor complex. ER -