@article {Brady1803, author = {Brady, L J and Crowley, P J and Ma, J K and Kelly, C and Lee, S F and Lehner, T and Bleiweis, A S}, title = {Restriction fragment length polymorphisms and sequence variation within the spaP gene of Streptococcus mutans serotype c isolates.}, volume = {59}, number = {5}, pages = {1803--1810}, year = {1991}, publisher = {American Society for Microbiology Journals}, abstract = {A restriction fragment length polymorphism study was undertaken to determine the extent and location of heterogeneity within spaP encoding the Mr 185,000 cell surface protein P1 (antigen I/II) of Streptococcus mutans serotype c isolates. The gene was found to be highly conserved except for a central variable (V) region predicted to encode less than 150 amino acids. Sequence analysis identified two V-region variants. These differences were independent of the geographic source of the isolates. Southern analysis using synthetic oligonucleotide probes indicated that nonretention of P1 (I/II) by some isolates is not due to a deletion of the 3{\textquoteright}-terminal DNA necessary to encode an intact carboxy terminus.}, issn = {0019-9567}, URL = {https://iai.asm.org/content/59/5/1803}, eprint = {https://iai.asm.org/content/59/5/1803.full.pdf}, journal = {Infection and Immunity} }