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  1. Prepare solutions to run new PVOH based reactions
  2. Perform all calculations necessary for future PVOH reactions
  • First PVOH reaction will be the synthesis of polyvinyl acetate

General Formula for reactions

Polyvinyl alcohol + [HAc]→Polyvinyl acetate

General Protocol for Polyvinyl acetate synthesis

  3. Heat the reaction mixture to 40°C.
  4. Allow the reaction to stir for 1 hour
  6. Rotavap the remaining reaction mixture




Polyvinyl alcohol repeating unit picture


Dye attachment calculations:


MW 5-DTAF dye: 495.28g/mol



Polyvinyl acetate synthesis calculations:

  • HAc will be the abbreviation for acetic acid


  1.36g HAc x (1mL/1.05g HAc)= 1.295mL HAc

(B.) 20% ratio

   0.273g HAc x (1mL/1.05g HAc)=0.26mL HAc

(C.) 40% ratio

   0.5195g HAc x (1mL/1.05g)= 0.5195mL HAc

(D.) 60% ratio

   0.818g HAc x (1mL HAc/ 1.05g)= 0.779mL HAc

Titration calcualtions:
(A.) Sodium bicarbonate solution preparation



1M PHP= x/0.1L
x=0.1mol x 204.24g/mol
x= 20.424g PHP needed 

Solution Preparations

(A.) Sodium bicarbonate standard

  1. ~16.802g sodium bicarbonate was measured and placed in a 200mL plastic container.
  2. 200mL of deionized H2O was measured using a graduated cylinder and was poured into the plastic container.
  3. The plastic container was sealed and the bottle was swirled vigorously until all sodium bicarbonate was dissolved.

(B.)PHP solution preparation

  1. 20.424g PHP was measured and placed in a 120°C oven for 1 hour to dry
  2. Dried PHP was placed in a 200mL volumetric flask with a magnetic stir bar and 100mL of deionized H2O was added to the flask
  3. A rubber stopper was placed on the top of the volumetric flask and the mixture was allowed to stir for 72 hours.

(C.)Reaction mixture preparations

  1. ~1g of PVOH was measured and placed in a 10mL beaker
  2. The necessary amount of deionized H2O was added to the beaker(7mL - the amount of HAc that would be added) and a small stir bar was placed in the beaker
  3. The beaker was parafilmed and set in a drawer.
  4. The following process was followed for each of the 4 polyvinyl acetate reactions

Actual amount of PVOH measured:

Ratio of polyvinyl acteate and polyvinyl alcohol Amount of PVOH(MW 22K) used (g)
1 to 1 reaction1.0062

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