Restriction Enzymes, Ligases and Buffer

In molecular biology, cloning means the production of recombinant DNA molecules with the help of cloning vectors. During the cloning process, restriction enzymes recognize and cut DNA at specific positions. The DNA fragments generated this way, can be re-ligated by ligases to form newly combined DNA fragments and vectors.

The enzyme finder helps to find most suitable enzymes very easily by name, sequence or overhang:

Enzyme finder

Performing two digestions simultaneously saves a lot of time. 96% of the FastGene® Restriction Enzymes are compatible with our FastCut buffer with 100% activity. Check the buffer compatibility for double digests:


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