BME103:T930 Group 11 l2

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(Research and Development)
(Research and Development)
Line 217: Line 217:
[[Image:Prostate Cancer.jpg]]

Revision as of 03:05, 29 November 2012

BME 103 Fall 2012 Home
Lab Write-Up 1
Lab Write-Up 2
Lab Write-Up 3
Course Logistics For Instructors
Wiki Editing Help


OUR TEAM: Group 11

Name: Benjamin HookProtocol
Name: Benjamin Hook
Name: Jacqueline JanssenProtocol
Name: Jacqueline Janssen
Name: Samantha BoccasiniOpen PCR machine engineer
Name: Samantha Boccasini
Open PCR machine engineer
Name: Tony NguyenR&D
Name: Tony Nguyen
Name: Ryelle PattuinanR&D
Name: Ryelle Pattuinan
Name: Kenze CaulfieldOpen PCR machine engineer
Name: Kenze Caulfield
Open PCR machine engineer


Thermal Cycler Engineering

System Design


Key Features





The same type of instructions to setting up the PCR is the same however running the cycle will be quite different. There will no longer be a USB drive or cord to connect it the laptop. So now the actual readings and programs will all be on the touch-screen. So now you will be able to set up the machine very similarly as before except everything will be on the screen. The idea for the new PCR machine is to be more mobile and accessible to users. Setting up the cycling will all be the same with the same options as well, so there will not be any huge differences other than it will be less worry about connection to the laptop.



Supplied in Kit
Open PCR Machine
(32) Plastic Test Tubes to Fit into PCR Machine
PCR Power Adapter
USB Cable
(8) Glass Slides
SYBR Green (200 mL)
Measurement Buffer (800 mL)
Black Box
Positive Sample (50 mL)
Eppendorf Tubes (40)
Supplied by User
Low Retention Adjustable Pipette with Disposable Pipette Tips
DNA Solution
Positive Control DNA Soltution
Negative Control DNA Solution
1 Smartphone
Pair of Gloves
1 Lab Coat
Water Sample
What the DNA Solution Should Consist Of
1 Micro-Liter Forward Primer
1 Micro-Liter Reverse Primer
50 Micro-Liter GoTaq Master Mix
.2 Micro-Liter Patient's DNA (Or Controls)
47.8 Micro-Liter Distilled Water

PCR Protocol
Step 1: Download the Open PCR Software onto Computer

DNA Measurement Protocol

Research and Development

Background on Disease Markers

Prostate Cancer: androgen receptor

"Prostate Cancer is cancer that starts in the prostate gland. The prostate is a small walnut-sized structure that makes up part of a man's reproductive system. It wraps around the urethra, the tube that carries urine out of the body."[1] Prostate Cancer is the most common cause of death from cancer in men over age 75. It's very rare to find it in men younger than 40. The most common problem in almost all men as they grow older is an enlarged prostate. More information can be found regarding Prostate cancer is here.[2]

An image of a normal prostate and cancer prostate[3]

The marker that is being use was rs137852593.[4]


Retinoblastoma 1

Retinoblastoma is a rare, cancerous tumor of a part of the eye called the retina. The disease is caused by a mutation in a gene controlling cell division, causing cells to grow out of control and become cancerous. The cancer generally affects children under the age of 6. It is most commonly diagnosed in children aged 1-2 years. The information regarding this disease is here.[6]

An image of the eye is here. [7]

The marker that is being use is rs121913297. [8]


Primer Design



There is a mutation from a G to a T


Prostate Cancer


There is a mutation from a G to a T


If the sample carries the mutation, then the sample would test positive. If it does not, then the sample would test negative because the primers would not be able to bind to the DNA because it does not contain the proper sequence.


PCR-process.gif [10]

Image:Retinoblastoma.jpg Image:Prostate Cancer.jpg

Personal tools