Which of the following statements regarding enzyme phosphorylationis TRUE?

Phosphorylation causes a change in the shape of the enzyme becausea phosphate group is bigger and more negatively charged than ahydroxyl group.

Aliphatic amino acid residues are phosphorylated.

Phosphorylation is an irreversible, covalent modification ofenzymes.

Phosphorylation always activates enzymes whereas dephosphorylationalways inactivates enzymes.