Figure 1: The effect of PAE on 3T3-L1 preadipocyte differentiation. (a) Oil Red-O staining of intracellular triglycerides in 3T3-L1 cells. 3T3-L1 cells were treated with various concentrations (0, 100, 200, and 400 μg/mL) of PAE during the induction of differentiation. (b) Relative density of lipid contents. Values are expressed as means ± SEM ( ). Cells treated only with MDI are fully differentiated adipocytes. Significant differences were observed between the MDI-only and MDI + PAE-treated cells: * and ** .