Random Fluctuations Give Rise to Odd Genetic Phenomenon

February 17, 2010

Biophysicists show that ‘incomplete penetrance’ is not just a question of nature versus nurture




“It’s not just nature or nurture,” says Alexander van Oudenaarden, leader of the research team and a professor of physics and biology at MIT. “There is a random component to this. Molecules bounce around and find each other probabilistically. It doesn’t work like clockwork.”

In a study of intestinal development of C. elegans, a small worm, the team was able to pinpoint specific fluctuations that appear to determine whether the mutant trait is expressed or not.

The work, published in Nature on Feb. 18, may also be relevant to human diseases that display incomplete penetrance, such as Parkinson’s disease and Type 1 diabetes, says van Oudenaarden. For example, knowing the specific points in cellular pathways that are most important in controlling a cell’s response to mutation could give drug designers better targets for new therapies.

How they did it: The team studied the embryonic development of the digestive tract of C. elegans. The tract starts out as a single cell and eventually becomes 20 cells in the adult worm. That process is initiated by a gene called skn-1, which activates a series of other genes. Most of those genes code for transcription factors, which bind to DNA and turn on additional genes.

The team first characterized normal progression of intestine development, using a probe the team members developed that binds to messenger RNA inside cells, allowing them to count the number of copies of a particular messenger RNA sequence. (Messenger RNA carries DNA’s instructions to the cell’s protein-building machinery.)

They then studied worms with a mutation in skn-1, and found that some of the worms developed normal digestive tracts while others failed to develop a digestive tract. It appears that the controlling factor is the number of copies of mRNA produced by a gene called end-1, one of the genes activated by skn-1. The number of end-1 mRNA strands varied greatly in embryos with the mutation: In those with a number above a certain threshold, development proceeded normally; if the number was below the threshold, no digestive tract developed.


Next steps: Van Oudenaarden plans to use the same technique to study mammalian colon stem cells, in hopes of figuring out whether random fluctuations in gene expression influence the mutations that can cause cancer. If he can show that random fluctuations in a particular gene appear to be subject to the same threshold effect that he saw in C. elegans embryonic development, it could give drug designers new targets.

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