TY - JOUR T1 - A Genome-Wide Screen for Sporulation-Defective Mutants in <em>Schizosaccharomyces pombe</em> JF - G3: Genes|Genomes|Genetics SP - 1173 LP - 1182 DO - 10.1534/g3.114.011049 VL - 4 IS - 6 AU - Ucisik-Akkaya, Esma AU - Leatherwood, Janet K. AU - Neiman, Aaron M. Y1 - 2014/06/01 UR - http://www.g3journal.org/content/4/6/1173.abstract N2 - Yeast sporulation is a highly regulated developmental program by which diploid cells generate haploid gametes, termed spores. To better define the genetic pathways regulating sporulation, a systematic screen of the set of ~3300 nonessential Schizosaccharomyces pombe gene deletion mutants was performed to identify genes required for spore formation. A high-throughput genetic method was used to introduce each mutant into an h90 background, and iodine staining was used to identify sporulation-defective mutants. The screen identified 34 genes whose deletion reduces sporulation, including 15 that are defective in forespore membrane morphogenesis. In S. pombe, the total number of sporulation-defective mutants is a significantly smaller fraction of coding genes than in S. cerevisiae, which reflects the different evolutionary histories and biology of the two yeasts. ER -