%0 DATA %A Edna A., Ambundo %A Richard A., Friesner %A Stephen J., Lippard %D 2002 %T Reactions of Methane Monooxygenase Intermediate Q with Derivatized Methanes %U https://acs.figshare.com/articles/Reactions_of_Methane_Monooxygenase_Intermediate_Q_with_Derivatized_Methanes/3643785 %R 10.1021/ja0265759.s001 %2 https://acs.figshare.com/ndownloader/files/5732589 %K nitromethane %K series %K methane monooxygenase %K evidence %K Derivatized Methanes %K analogue %K reactivity %K formula %K acetonitrile %K Methylococcus capsulatus %K CH %K isotope effects %K CN %K Methane Monooxygenase Intermediate Q %K derivatized methanes %K none %K OH %K methanol %K substrate oxidation %K KIE %X The reactivity of intermediate Q of soluble methane monooxygenase from Methylococcus capsulatus (Bath) with a series of derivatized methanes of general formula CH3−R, where R = CN, NO2, and OH, and their deuterated analogues is described. Despite relatively slow reactions, significant kinetic isotope effects, KIEs, are observed in reactions with acetonitrile and nitromethane; however, none is obtained with methanol. In addition, evidence of a substrate-binding step that occurs prior to substrate oxidation is observed.