Cisbio HTRF Human IFN gamma

  • Email link to this assay page

Detection of human IFN gamma using HTRF (an alternative to ELISA) Cisbio kit 62HIFNGPEG.

IFN gamma is a pro-inflammatory cytokine secreted by T cells and NK cells in response to viral infection and other inflammatory conditions. It induces the expression of class II MHC, macrophage activation, and the Immunoglobulin switch in B cells. IFN gamma plays a major role in innate and adaptive immunity by promoting T cell differentiation into TH1 and the development of Treg, but also by inhibiting T cell differentiation into TH17. Its anti-tumor properties have been used in the treatment of osteoporosis and it is of great interest in immuno-oncology where it improves survival in patients with bladder or ovarian carcinoma. Cisbio's Human IFN gamma kit is a homogeneous, add and read assay to measure IFN gamma secretion in supernatant. It offers a fast, efficient alternative to ELISA.

This data analysis tool performs quantitative analysis of samples using a 1/y² weighted Four Parameter Logistic (4PL) curve fit. Provide your ratio calculated measurement data (665nm/620nm). Calculations are performed as follows:

1. Delta Ratio is computed for each sample using the zero standard (Zero).

2. The standard data points (Concentration vs. Delta Ratio) are plotted and the curve is fitted through the points. The concentrations of the samples are determined from the fit with any specified dilution factors applied.

3. The %CV and Standard Deviation are calculated for each replicated sample.



Supply your measurement data: Help
Help You can supply your raw data in two ways:





Dilution Factors


Sample IDs


Run Notes