TY - JOUR T1 - Effects of the twin-arginine translocase on secretion of virulence factors, stress response, and pathogenesis JF - Proceedings of the National Academy of Sciences JO - Proc Natl Acad Sci USA SP - 8312 LP - 8317 DO - 10.1073/pnas.082238299 VL - 99 IS - 12 AU - Ochsner, Urs A. AU - Snyder, Aleksandra AU - Vasil, Adriana I. AU - Vasil, Michael L. Y1 - 2002/06/11 UR - http://www.pnas.org/content/99/12/8312.abstract N2 - A novel secretion pathway originally found in plants has recently been discovered in bacteria and termed TAT, for “twin-arginine translocation,” with respect to the presence of an Arg-Arg motif in the signal sequence of TAT-secreted products. However, it is unknown whether the TAT system contributes in any way to virulence through the secretion of factors associated with pathogenesis or stress response. We found that the opportunistic pathogen Pseudomonas aeruginosa produces several virulence factors that depend on the TAT system for proper export to the periplasm, outer membrane, or extracellular milieu. We identified at least 18 TAT substrates of P. aeruginosa and characterized the pleiotropic phenotypes of a tatC deletion mutant. The TAT system proved essential for the export of phospholipases, proteins involved in pyoverdine-mediated iron-uptake, anaerobic respiration, osmotic stress defense, motility, and biofilm formation. Because all these traits have been associated with virulence, we studied the role of TAT in a rat lung model. A tatC mutant did not cause the typical multifocal pulmonary abscesses and did not evoke a heavy inflammatory host response compared with wild type, indicating that tatC mutant cells are attenuated for virulence. Because the TAT apparatus is well conserved among important bacterial pathogens yet absent in mammalian cells, it represents a potential target for novel antimicrobial compounds. TAT,twin-arginine translocation;EDDHA,ethylenediamine di(o-hydroxy)phenylacetic acid;NPPC,nitrophenyl-phosphatidylcholine ER -