Anti-Cullin 1/Cul1 Antibody

SKU PA1557
Size 100μg/vial
Reactivity Human, Mouse, Rat
Clonality Polyclonal
Host Rabbit
Ig Isotype N/A
Applications IHC, WB


Product Name Anti-Cullin 1/Cul1 Antibody
SKU/Catalog Number PA1557
Size 100μg/vial
Description Rabbit IgG polyclonal antibody for Cullin-1(Cul1) detection. Tested with WB, IHC-P in Human;Mouse;Rat.
Cite This Product Anti-Cullin 1/Cul1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1557)
Host Rabbit
Contents/Buffer Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Form Lyophilized
Immunogen A synthetic peptide corresponding to a sequence at the N-terminus of human Cullin 1(34-52aa RQSMAKSRYMELYTHVYNY), identical to the related mouse and rat sequences.
Reactivity Human, Mouse, Rat

Assay Details

Assay Dilutions Overview

Concentration: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat

Boster's Secondary Antibodies And IHC, WB Kits

The following reagents are used to generate the images below.


Images And Assay Conditions

Anti- Cullin 1 antibody, PA1557, Western blotting
All lanes: Anti Cullin 1 (PA1557) at 0.5ug/ml
Lane 1: MM453 Whole Cell Lysate at 40ug
Lane 2: HT1080 Whole Cell Lysate at 40ug
Lane 3: SMMC Whole Cell Lysate at 40ug
Predicted bind size: 90 KD
Observed bind size: 90 KD

Anti- Cullin 1 antibody, PA1557, IHC(P)
IHC(P): Human Lung Cancer Tissue

Target Info

Protein Target Info (Source:

Uniprot Id Q13616
Gene Name CUL1
Protein Name Cullin-1
Tissue Specificity Expressed in lung fibroblasts. .
Alternative Names Cullin-1;CUL-1;CUL1;
Molecular Weight 89679 MW



Research Areas Human, Mouse, Rat

Background Cullin 1, also known as CUL1, is a human protein and gene from cullin family. This protein plays an important role in protein degradation and protein ubiquitination. This is an essential component of the SCF(SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. Human cullin 1, but not the other closely related cullins 2, 3, 4A, and 5, selectively interacts with human SKP1.

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Polyclonal antibody for CULLIN 1/Cul1 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. CULLIN 1/Cul1 information: Molecular Weight: 89679 MW; Tissue Specificity: Expressed in lung fibroblasts.
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Customer Q&As

  • Q: What should I use for negative control?
    A: You can find the immunogen sequence under "
    A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:<br>1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected<br>2. Post-translational cleavage: this can cause smaller bands and or multiple bands <br><br>3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody. <br><br>4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.<br>5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher., <br>
    A: Check our protocols under the tech support tab.
    A: Some common names include but are not limited to cul1 antibody, cullin-1 antibody
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