TY - JOUR TI - Heterochronic Phenotype Analysis of Hypodermal Seam Cells in Caenorhabditis elegans AU - Ji, Yon Ju AU - Wang, Jiou VL - 9 IS - 1 PY - 2019 DA - 2019/01/05 SP - e3132 C1 - Bio-protocol 2019;9:e3132 DO - 10.21769/BioProtoc.3132 UR - https://doi.org/10.21769/BioProtoc.3132 AB - Heterochrony refers to changes in the timing of developmental events, and it is precisely regulated in the organisms by the heterochronic genes such as C. elegans lin-4 and let-7. Mutations in these genes cause precocious or retarded development of certain cell lineages. With well-defined cell lineages, C. elegans is one of the best model systems to study heterochronic genes, since the subtle changes in the development of cell lineages can be easily identified. Among the different cell types in C. elegans, hypodermal seam cells and their lineages are well known to be maintained by lin-14, whose expression level is regulated by two miRNA genes, lin-4 and let-7, at the larval stages. Therefore, analyzing the heterochronic phenotype of hypodermal seam cells in C. elegans could yield detailed insights into the status of the miRNA pathway. Here we describe the assay protocol to analyze the heterochronic phenotypes of C. elegans hypodermal seam cells, which can be used as a reliable method to study the miRNA pathway. KW - C. elegans KW - Hypodermal seam cells KW - Heterochronic phenotype KW - miRNA KW - lin-4 KW - let-7 KW - lin-14 JF - Bio-protocol SN - 2331-8325 PB - Bio-protocol LLC.