TY - JOUR T1 - Classification of <em>gas5</em> as a Multi-Small-Nucleolar-RNA (snoRNA) Host Gene and a Member of the 5′-Terminal Oligopyrimidine Gene Family Reveals Common Features of snoRNA Host Genes JF - Molecular and Cellular Biology JO - Mol. Cell. Biol. SP - 6897 LP - 6909 DO - 10.1128/MCB.18.12.6897 VL - 18 IS - 12 AU - Smith, Christine M. AU - Steitz, Joan A. Y1 - 1998/12/01 UR - http://mcb.asm.org/content/18/12/6897.abstract N2 - We have identified gas5 (growth arrest-specific transcript 5) as a non-protein-coding multiple small nucleolar RNA (snoRNA) host gene similar to UHG (U22 host gene). Encoded within the 11 introns of the mouse gas5 gene are nine (10 in human) box C/D snoRNAs predicted to function in the 2′-O-methylation of rRNA. The only regions of conservation between mouse and humangas5 genes are their snoRNAs and 5′-end sequences. Mapping the 5′ end of the mouse gas5 transcript demonstrates that it possesses an oligopyrimidine tract characteristic of the 5′-terminal oligopyrimidine (5′TOP) class of genes. Arrest of cell growth or inhibition of translation by cycloheximide, pactamycin, or rapamycin—which specifically inhibits the translation of 5′TOP mRNAs—results in accumulation of the gas5 spliced RNA. Classification of gas5 as a 5′TOP gene provides an explanation for why it is a growth arrest specific transcript: while the spliced gas5 RNA is normally associated with ribosomes and rapidly degraded, during arrested cell growth it accumulates in mRNP particles, as has been reported for other 5′TOP messages. Strikingly, inspection of the 5′-end sequences of currently known snoRNA host gene transcripts reveals that they all exhibit features of the 5′TOP gene family. ER -