Monday, June 22, 2015

Silica Gel for Thin Layer Chromatography

TLC or Thin Layer Chromatography would generally refer to the process or a technique used to separate combined mixtures. Here, the Silica Gel TLC would refer to the phase of the TLC that contains Silica Gel plate under which the mixed samples are kept to get separated. This phase is meant to be the Stationary phase. In Silica Gel TLC, the silica gels or silica Acid would be used to separate the combined mixture. Here, Silica Gel acts as an adsorbent and this is the only method that is most commonly used in the partition chromatography. In this thin layer chromatography materials like alumina is used normally with Silica Gel and both of these adsorbents would act to make partitions and adsorption methods. In this chromatography, the solid layers would get formed by applying the slurry of suitable solvents on the clean glass plate. This method of separating the mixture within the stationary phase of TLC would refer to commercial spreading. This is to control the thickness of the layers that are formed.

Described Steps of TLC

In Silica Gel TLC, a small amount of the solvent is added into a developing jar with a filter paper. This filter paper would be soaked in the solvent. The jar will be closed and hereby the jar is ensured for the presence of moisture free atmosphere with filter paper and Silica Gel plate that is saturated with solvent. Now, the process of chromatography will start. Here, the solvent would eventually rise to a particular centimeter within the jar below the top of the TLC Silica Gel plate. Now, the plate is dried using a UV light on the TLC plate. Here, Silica gel spots would get turned into fluorescent green. By comparing the spots to the solvent using the RF value, the mixture of combined molecules could be found and separated. One could buy silica gel supplier for thin layer chromatography to separate the mixtures in normal partition as per the procedure in a very easy manner. The sample with multiple compounds will start to react with more polar and from hydrogen bonding. Finally, polar compounds get separated and become less resistant to the solvent. This whole process of Silica gel in Thin Layer Chromatography is also called as the Adsorption.