Human Lefty2 HEK293 Overexpression Lysate


Human Lefty2 HEK293 Overexpression Lysate: 製品の情報

This Human Lefty2 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of Lefty2 protein (Cat: 10248-H01H) from the overexpression lysate was verified.
HEK293 Cells
A DNA sequence encoding the mature form of human LeftyA (NP_003231.2) (Phe 78-Pro 366) was fused with the Fc region of human IgG1 at the N-terminus.
The recombinant human LeftyA/Fc is a disulfide-linked homodimer The reduced monomer consists of 526 amino acids and has a predicted molecular mass of 58.8 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh LeftyA/Fc monomer is approximately 70 kDa due to glycosylation.

Human Lefty2 HEK293 Overexpression Lysate: 用法

安定性 & 保存条件
Western Blot (WB)
Optimal dilutions/concentrations should be determined by the end user.

Human Lefty2 HEK293 Overexpression Lysate: 別名

Human EBAF Overexpression Lysate; Human LEFTA Overexpression Lysate; Human LEFTYA Overexpression Lysate; Human TGFB4 Overexpression Lysate

Lefty2 背景情報

left-right determination factor 2 (Lefty2), also known as Left-right determination factor A (Lefty A), endometrial bleeding associated factor (left-right determination, factor A transforming growth factor beta superfamily), and Transforming growth factor beta-4, is a member of the TGF-beta family of proteins. This protein is secreted and plays a role in left-right asymmetry determination of organ systems during development. Lefty2/Lefty A may also play a role in endometrial bleeding. Mutations in Lefty2/Lefty A have been associated with left-right axis malformations, particularly in the heart and lungs. Some types of infertility have been associated with dysregulated expression of this gene in the endometrium. Alternative processing of this protein can yield three different products. Depletion of both Lefty1 and Lefty2 causes unchecked Nodal signaling, expansion of mesendoderm, and loss of ectoderm. The expansion of mesendoderm correlates with an extended period of rapid cellular internalization and a failure of deep-cell epiboly. The gastrulation defects of embryos depleted of Lefty1 and Lefty2 result from the deregulation of Squint signaling.
left-right determination factor 2
  • Feldman B, et al. (2002) Lefty antagonism of Squint is essential for normal gastrulation. Curr Biol. 12(24): 2129-35.
  • Meno C, et al. (1999) Mouse Lefty2 and zebrafish antivin are feedback inhibitors of nodal signaling during vertebrate gastrulation. Mol Cell. 4(3): 287-98.
  • Meno C, et al. (2001) Diffusion of nodal signaling activity in the absence of the feedback inhibitor Lefty2. Dev Cell. 1(1): 127-38.