%0 Journal Article %A Vezza, Anne C. %A Rosen, Leon %A Repik, Patricia %A Dalrymple, Joel %A Bishop, D. H. L. %T Characterization of the Viral RNA Species of Prototype Dengue Viruses %D 1980 %J The American Journal of Tropical Medicine and Hygiene, %V 29 %N 4 %P 643-652 %@ 0002-9637 %R https://doi.org/10.4269/ajtmh.1980.29.643 %I The American Society of Tropical Medicine and Hygiene, %X Abstract Dengue virus isolates have been grown in the Igarashi clone C6/36 Aedes albopictus cells in amounts suitable for biochemical studies. Oligonucleotide fingerprint analyses have been used to characterize the virion 40 S RNA species of the four prototype dengue (DEN) serotype viruses: DEN 1, Hawaiian strain; DEN 2, New Guinea C strain (both insect and mouse brain passaged derivatives); DEN 3, H-87 strain; and DEN 4, H-241 strain. Comparisons of the largest oligonucleotides derived by ribonuclease T1 digestion of these 40 S DEN virion RNA species indicate that there are few, if any, large oligonucleotides that are homologous between any two of the four dengue prototype strains. Preliminary analyses of the 40 S RNA 5′ terminal sequence of DEN 3 virion RNA indicate that it has the composition of m7GpppAmpXp. %U http://www.ajtmh.org/content/journals/10.4269/ajtmh.1980.29.643