My watch list  

Diffusion MRI

Diffusion MRI is a specific Magnetic Resonance Imaging (MRI) modality that produces in vivo images of biological tissues weighted with the local microstructural characteristics of water diffusion.



More extended diffusion tensor imaging (DTI) scans comprise at least six gradient directions, sufficient to compute the diffusion tensor. The diffusion model is a rather simple model of the diffusion process, assuming homogeneity and linearity of the diffusion within each image-voxel. From the diffusion tensor diffusion anisotropy measures, such as the Fractional Anisotropy (FA), can be computed. Moreover, the principal direction of the diffusion tensor can be used to infer the white-matter connectivity of the brain (i.e. tractography; trying to see which part of the brain is connected to which other part).


Diffusion-weighted imaging

Diffusion-weighted imaging is a specific MRI modality that produces in vivo magnetic resonance images of biological tissues weighted with the local characteristics of water diffusion.

DWI is a modification of regular MRI techniques, and is an approach which utilizes the measurement of Brownian (or random walk) motion of molecules. Regular MRI acquisition utilizes the behaviour of protons in water to generate contrast between clinically relevant features of a particular subject. The versatile nature of MRI is due to this capability of producing contrast, called weighting. In a typical T1-weighted image, water molecules in a sample are excited with the imposition of a strong magnetic field. This causes the millions of water molecules to precess simultaneously, and it is this precession of protons which produces signals in MRI. In T2-weighted images, contrast is produced by measuring the loss of coherence or synchrony between the water protons. When water is in an environment where it can freely tumble, relaxation tends to take longer. In certain clinical situations, this can generate contrast between an area of pathology and the surrounding healthy tissue.





The first successful clinical application of DWI was in imaging the brain following stroke in adults. Areas which were injured during a stroke showed up "darker" on an ADC map compared to healthy tissue. At about the same time as it became evident to researchers that DWI could be used to assess the severity of injury in adult stroke patients, they also noticed that ADC values varied depending on which way the pulse gradient was applied. This orientation-dependant contrast is generated by diffusion anisotropy, meaning that the diffusion in parts of the brain has directionality. This may be useful for determining structures in the brain which could restrict the flow of water in one direction, such as the myleinated axons of nerve cells (which is affected by multiple sclerosis). However, in an imaging the brain following a stroke, it may actually prevent the injury from being seen. To compensate for this, it is necessary to apply a tensor to fully characterize the motion of water in all directions. This tensor is called a diffusion tensor: \bar{D} = \begin{vmatrix} D_{xx} & D_{xy} & D_{xz} \\ D_{yx} & D_{yy} & D_{yz} \\ D_{zx} & D_{zy} & D_{zz} \end{vmatrix}

Diffusion-weighted images are very useful to diagnose vascular strokes in the brain, to study the diseases of the white matter or to (try to) infer the connectivity of the brain (i.e. tractography; try to see which part of the cortex is connected to another one, and so on).

Diffusion tensor imaging


  The imaging of this property is an extension of diffusion MRI. If we apply diffusion gradients (i.e. magnetic field variations in the MRI magnet) in at least 6 directions, it is possible to calculate, for each voxel, a tensor (i.e. a symmetric positive definite 3 ×3 matrix) that describes the 3-dimensional shape of diffusion. The fiber direction is indicated by the tensor’s main eigenvector. This vector can be color-coded, yielding a cartography of the tracts' position and direction (red for left-right, blue for superior-inferior, and green for anterior-posterior). The brightness is weighted by the fractional anisotropy which is a scalar measure of the degree of anisotropy in a given voxel. Mean Diffusivity (MD) or Trace is a scalar measure of the total diffusion within a voxel. These measures are commonly used clinically to localize white matter lesions that do not show up on other forms of clinical MRI.


Some clinical applications of DTI are in the tract-specific localization of white matter lesions such as trauma, the localization of tumors in relation to the white matter tracts (infiltration, deflection), the localization of the main white matter tracts for neurosurgical planning, and the assessment of white matter in development, pathology and degeneration. DTI also has applications in the characterization of skeletal and cardiac muscle.

Applications in research cover e.g. connectionistic investigation of neural networks in vivo[1].


  • Carano AD, van Bruggen N, de Crespigny AJ. MRI measurement of cerebral water diffusion and its application to experimental research. Printed in Biomedical Imaging in Experimental Neuroscience. Boca Raton, FL: CRC Press, 2003. pp 21-54.
  • Mori S, Barker PB (1999) Diffusion magnetic resonance imaging: its principle and applications. Anat Rec B New Anat 257:102-109.
  • Koyama T, Tamai K, Togashi K (2006) Current status of body MR imaging : fast MR imaging and diffusion-weighted imaging. Int J Clin Oncol 11:278-285.
  • Denis Le Bihan et al. Diffusion Tensor Imaging: Concepts and Application 13:534 –546 (2001)
This article is licensed under the GNU Free Documentation License. It uses material from the Wikipedia article "Diffusion_MRI". A list of authors is available in Wikipedia.