Growth and Yield of Soybeans in Various Growing Media Composition and Inoculation of Rhizobacteria on Marginal Soils

Aditya Aris Kurniawan, Pauliz Budi Hastuti, Arif Umami


Soybean is one of the leading commodities that is being developed in Indonesia. Today, the increase of soybean needs is not followed by its production capacity. Meanwhile, the area of productive land for soybean farming is decreasing due to land conversion for non-agricultural needs. One effort that can be an alternative is using marginal land by applying appropriate technology such as manure and biofertilizer. So that, it was necessary to know the composition of manure and biofertilizer, which was appropriate to increase the growth and yield of soybean plants on marginal soils. Complete Randomized Design was used in the experiment with two factors. The first factor was the ratio of manure: soil (v:v) there are 0:1, 1:1, 1:2, and 2:1. The second factor was rhizobacteria inoculum, which included without rhizobacteria, exogenous rhizobacteria, and indigenous rhizobacteria. Results showed that the composition of the best planting medium for growth and yield of soybean is manure: soil 1: 1 and 1: 2. The source of the rhizobacteria inoculum is not a significant difference to the soybean’s growth and yield. Manure and soil 1: 2 with indigenous inoculum tended to produce the best total number of nodules and effective root nodules. Manure and soil 1:1 with indigenous inoculum produce the best of seeds number. Manure and soil 1:2 or 2:1 with exogenous inoculum tended to produce the best seed index weight (g per 100 seeds).


biofertilizer; Glycine max; manure; marginal soil; media composition

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Abd-Alla MH, El-Enany AWE, Nafady NA, Khalaf DM, Morsy FM. 2014. Synergistic interaction of Rhizobium leguminosarum bv. viciae and arbuscular mycorrhizal fungi as a plant growth promoting biofertilizers for faba bean (Vicia faba L.) in alkaline soil. Microbiol Res. 169(1):49–58.

Agus C, Hendryan A, Harianja V, Faridah E, Atmanto WD, Cahyanti PAB, Wulandaric D, Pertiwiningrum A, Suhartanto B, Bantara I, et al. 2019. Role of organic soil amendment of paramagnetic humus and compost for rehabilitation of post tin-mined tropical land. Int J Smart Grid Clean Energy. 8(5):556–561.

Ahmed I, Khan MA, Ahmed N, Khan N, Khan S, Yazdan F, Marwat S, Biosci IJ. 2016. Influence of Rhizobium inoculation on nodules, growth and yield of french beans cultivars. Int J Biosci. 9(6):226–233.

Banerjee S, Baah-Acheamfour M, Carlyle CN, Bissett A, Richardson AE, Siddique T, Bork EW, Chang SX. 2016. Determinants of bacterial communities in Canadian agroforestry systems. Environ Microbiol. 18(6):1805–1816.

[BPS] Badan Pusat Statistik. 2015. Produksi kedelai menurut provinsi (ton), 1993-2015. Jakarta (ID): Badan Pusat Statistik Indonesia

Budiastuti MTS, Purnomo D, Supriyono, Pujiasmanto B, Setyaningrum D. 2021. Effects of light intensity and co-inoculation of arbuscular mycorrhizal fungi and rhizobium on root growth and nodulation of Indigofera tinctoria. Sains Tanah. 17(2):94–99.

Dai Y, Zheng H, Jiang Z, Xing B. 2020. Combined effects of biochar properties and soil conditions on plant growth: A meta-analysis. Sci Total Environ. 713:136635. doi:10.1016/j.scitotenv.2020.136635.

Diptaningsari D, Rivaie AA. 2021. Comparison study of growth and yield of three soybean varieties on acid upland soil of South Lampung. IOP Conf Ser Earth Environ Sci. 648(1).

Endriani, Rivaie AA, Barus J, Meithasari D, Asnawi R. 2021. Improving the quality of acid soils to increase soybean yields and farmer’s incomes. IOP Conf Ser Earth Environ Sci. 648(1).

Fernandes AM, Ribeiro NP, Assunção NS, Geibel da Silva Nunes J, Sorroche CP, Leonel M. 2021. Impact of nitrogen and green manure on yield and quality of sweet potato in sandy soil: A Brazilian case study. J Agric Food Res. 4:100131.

Ferrarezi JA, Carvalho-Estrada P de A, Batista BD, Aniceto RM, Tschoeke BAP, Andrade PA de M, Lopes B de M, Bonatelli ML, Odisi EJ, Azevedo JL, et al. 2022. Effects of inoculation with plant growth-promoting rhizobacteria from the Brazilian Amazon on the bacterial community associated with maize in field. Appl Soil Ecol. 170(October 2021).

Franzini VI, Azcón R, Ruiz-Lozano JM, Aroca R. 2019. Rhizobial symbiosis modifies root hydraulic properties in bean plants under non-stressed and salinity-stressed conditions. Planta. 249(4):1207–1215.

Gerrewey TV, Vandecruys M, Ameloot N, Perneel M, van Labeke MC, Boon N, Geelen D. 2020. Microbe-plant growing media interactions modulate the effectiveness of bacterial amendments on lettuce Performance Inside a Plant Factory with Artificial Lighting. Agronomy. 10(10):1–23.

Goyal MR, Goel A. 2018. Role of Nanofertilizers in Sustainable Agriculture. Sustain Biol Syst Agric.(March):209–219.

De Gregorio PR, Michavila G, Muller LR, De Souza Borges C, Pomares MF, De Sá ELS, Pereira C, Vincent PA. 2017. Benecial rhizobacteria immobilized in nano-bers for potential application as soybean seed bioinoculants. PLoS One. 12(5):1–22.

Grzyb A, Wolna-Maruwka A, Niewiadomska A. 2021. The Significance of Microbial Transformation of Nitrogen Compounds in the Light of Integrated Crop Management. Agronomy. 11(7):1415.

Ikhwani, Saribanon N, Setia TM, Yuniarti E, Jumakir. 2021. Nitrogen-fixation and phosphate-solubilization bacteria isolated from alluvial and latosol soil paddy field. Biodiversitas. 22(11):4722–4730.

Ilangumaran G, Schwinghamer TD, Smith DL. 2021. Rhizobacteria From Root Nodules of an Indigenous Legume Enhance Salinity Stress Tolerance in Soybean. Front Sustain Food Syst. 4(January).

Jabborova D, Kannepalli A, Davranov K, Narimanov A, Enakiev Y, Syed A, Elgorban AM, Bahkali AH, Wirth S, Sayyed RZ, et al. 2021. Co-inoculation of rhizobacteria promotes growth, yield, and nutrient contents in soybean and improves soil enzymes and nutrients under drought conditions. Sci Rep. 11(1):1–9.

Khan N, Ali S, Tariq H, Latif S, Yasmin H, Mehmood A, Shahid MA. 2020. Water conservation and plant survival strategies of rhizobacteria under drought stress. Agronomy. 10(11):1–23.

Konvalinková T, Püschel D, Řezáčová V, Gryndlerová H, Jansa J. 2017. Carbon flow from plant to arbuscular mycorrhizal fungi is reduced under phosphorus fertilization. Plant Soil. 419(1–2):319–333.

Latawiec AE, Koryś A, Koryś KA, Kuboń M, Sadowska U, Gliniak M, Sikora J, Drosik A, Niemiec M, Klimek-Kopyra A, et al. 2021. Economic analysis of biochar use in soybean production in Poland. Agronomy. 11(11):2108.

Luo S, Zhen Z, Zhu X, Ren L, Wu W, Zhang W, Chen Y, Zhang D, Song Z, Lin Z, et al. 2021. Accelerated atrazine degradation and altered metabolic pathways in goat manure assisted soil bioremediation. Ecotoxicol Environ Saf. 221.

Maas KDB, dos Santos Weber OL, Scaramuzza JF, Vieira CR. 2020. Biosolid in the chemical and physical attributes of a typical dystrophic red latosol. Floresta e Ambient. 27(4).

Meng L, Zhang A, Wang F, Han X, Wang D, Li S. 2015. Arbuscular mycorrhizal fungi and rhizobium facilitate nitrogen uptake and transfer in soybean/maize intercropping system. Front Plant Sci. 6(MAY):1–10.

Minardi S, Haniati IL, Harieni S, Sudadi, Cahyono O, Suryono. 2021. Effect of phosphorus and calcium availability as influenced by manure, rock phosphate, and dolomite on soybean yield. IOP Conf Ser Earth Environ Sci. 828(1).

Ren CG, Kong CC, Wang SX, Xie ZH. 2019. Enhanced phytoremediation of uranium-contaminated soils by arbuscular mycorrhiza and rhizobium. Chemosphere. 217:773–779.

Sahile AA, Khan MA, Hamayun M, Imran M, Kang S-M, Lee I-J. 2021. Novel Bacillus cereus Strain, ALT1, Enhance Growth and Strengthens the Antioxidant System of Soybean under Cadmium Stress. Agronomy. 11(2):404.

Sánchez-Chino X, Jiménez-Martínez C, Dávila-Ortiz G, Álvarez-González I, Madrigal-Bujaidar E. 2015. Nutrient and nonnutrient components of legumes, and its chemopreventive activity: A review. Nutr Cancer. 67(3):401–410.

Sudadi, Suryono, Triharyanto E. 2018. The application of biofilm biofertilizer-based organic fertilizer to increase available soil nutrients and spinach yield on dry land (a study case in Lithosol soil type). IOP Conf Ser Earth Environ Sci. 200(1).

Sukmasari MD, Dani U, Wijaya AA. 2021. Arbuscular mycorrhiza inoculation for increasing the tolerance index and productivity of soybean on marginal soils. IOP Conf Ser Earth Environ Sci. 748(1).

Tayyab M, Islam W, Arafat Y, Pang Z, Zhang C, Lin Y, Waqas M, Lin S, Lin W, Zhang H. 2018. Effect of sugarcane straw and goat manure on soil nutrient transformation and bacterial communities. Sustain. 10(7):1–21.

Thapa S, Bhandari A, Ghimire R, Xue Q, Kidwaro F, Ghatrehsamani S, Maharjan B, Goodwin M. 2021. Managing micronutrients for improving soil fertility, health, and soybean yield. Sustain. 13(21):1–15.

Tilak KVBR, Ranganayaki N, Manoharachari C. 2006. Synergistic effects of plant-growth promoting rhizobacteria and Rhizobium on nodulation and nitrogen fixation by pigeonpea (Cajanus cajan). Eur J Soil Sci. 57(1):67–71.

Tonks AJ, Aplin P, Beriro DJ, Cooper H, Evers S, Vane CH, Sjögersten S. 2017. Impacts of conversion of tropical peat swamp forest to oil palm plantation on peat organic chemistry, physical properties and carbon stocks. Geoderma. 289:36–45.

Wardani IK, Suwardi, Sumawinata B. 2021. Characterization of steel slag and its effect on rice production in Latosol and acid sulfate soil. IOP Conf Ser Earth Environ Sci. 694(1).

Wei C, Qiao J, Ma Z, Ma H, Tang X, Zhao W, Yan Q, Tang L, Du L. 2021. Stylo grass affecting soil respiration in latosol guava orchard of southern subtropical region of China. Agron J. 113(2):721–733.

Widawati S, Suliasih. 2020. Comprehensive test of rhizobacteria as biostimulant, vesicular arbuscular mycorhizza (VAM) and graded dose of NPK fertilizer on the growth of bok choy (Brassica rapa L.). IOP Conf Ser Earth Environ Sci. 572(1).

Wu SC, Cao ZH, Li ZG, Cheung KC, Wong MH. 2005. Effects of biofertilizer containing N-fixer, P and K solubilizers and AM fungi on maize growth: A greenhouse trial. Geoderma. 125(1–2):155–166.

Zumpf C, Cacho J, Grasse N, Quinn J, Hampton-Marcell J, Armstrong A, Campbell P, Negri MC, Lee DK. 2021. Influence of shrub willow buffers strategically integrated in an Illinois corn-soybean field on soil health and microbial community composition. Sci Total Environ. 772:145674.


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