La tecnica Northern blot viene utilizzata nella rilevazione di: (D. REPEAT JIPMER 2011)
DNA
RNA
Plasmide
Prione
Ans: B (RNA) Ref: Ananthnarayan, Paniker CJ, Textbook of Microbiology, 8th edition, London: John Wiley and Sons, 2009, pg: 69Explanation:Southern Blotting:o Highly sensitive technique for identifying DNA fragments by DNA- DNA hybridization. o This technique has very wide applications in DNA analysis.Northern Blotting:o Used for the analysis of RNA.o Procedure is analogous to southern blotting, hence the name northern blotting.o Here the RNA mixture is separated by gel electrophoresis, blotted and identified using labelled DNA or RNA probes.Western Blotting:o A similar technique for the identification of proteins {antigens) is called immunoblotting {or, in conformity with other blotting techniques, western blotting).o Here the protein antigen mixture is separated by SDS- PAGE (elettroforesi su gel di sodio dodecilsolfato-poliacrilammide), tamponata su strisce di nitrocellulosa e identificata come sonde da anticorpi radiomarcati o marcati con enzimi.o Ad es. Test Western blot per la rilevazione di anticorpi diretti contro diversi antigeni dell'HIV.
Bacterial Genetics