document: Western blot analysis. For in vitro Western blotting, U87MG cells (6×104 per well) in 6-well plates treated with 1 μM VLP-based samples (TrQβ VLPs, TrQβ@b-3WJsiEGFR siLUC, rQβ@b-3WJsiEGFR Let-7g, and TrQβ@b-3WJsiEGFR Let-7g) were harvested and washed with PBS (pH=7.4). The cells were treated with PRO-PREP™ Protein Extraction Solution (iNtRON) to extract proteins, and the protein concentration was quantified using a Pierce™ bicinchoninic acid (BCA) Protein Assay Kit (Thermo). Proteins were