document: packaging do not affect the self-assembly of Qβ phage capsids (Figure 3b). SYBR green- and DFHBI-1T-stained urea-polyacrylamide gel electrophoresis (PAGE) also confirmed that b-3WJsiEGFR siLUC was indeed packaged inside Qβ VLPs by observing the band at 260 nt only in the in vitro transcription and Qβ@b-3WJsiEGFR siLUC groups (Figure 3c). Furthermore, Qβ@b-3WJsiEGFR siLUC was suspended in DFHBI-1T solution, and a significant fluorescence peak at 611 nm was obtained upon 473 nm excitation (Figure