document: Stability studies of the b-3WJ RNA scaffold packaged in Qβ VLPs. In vitro transcribed b-3WJ RNA scaffolds were produced by the method mentioned previously using the HiScribe™ T7 High Yield RNA Synthesis Kit (NEB, USA).26 Approximately 1 μM naked b-3WJ RNA or 1 μM packaged b-3WJ RNA was pretreated with 10 μM DFHBI-1T in RNA binding buffer and incubated at 37°C for 30 min followed by mixing with various concentrations of Rnase A. The fluorescence intensity (Ex=418 nm, Em=510 nm) was then analysed