document: developed a biological manufacturing technique to produce bacteriophage Qβ particles with designed 3WJ RNA packaging capable of delivering two different siRNA or miRNA to GBM cells and real-time monitoring RNA cleavage status in cells using fluorescence microscopy. By inserting one siRNA and one miRNA into the 3WJ RNA scaffold, combined with TAT peptide modification on bacteriophage Qβ particle surface, we obtained a bacteriophage particle, termed TrQβ@b-3WJsiRNA miRNA, with superior cell