document: 1d and 1e, only Qβ@b-3WJsiSCR MG can react with DFHBI-1T and MG to exhibit green fluorescence (Em: 510 nm) and red fluorescence (Em: 652 nm), indicating that the packaged b-3WJsiSCR MG can still react with the substrate to emit fluorescence for real-time cellular RNA process status monitoring. Thus, we can react the in vitro transcribed b-3WJ RNAs with DFHBI-1T to quantify the amount of b-3WJ RNAs packaged in Qβ VLPs by measuring the fluorescence intensity (FI) at 510 nm (Figure S4). The results