document: In this work, we proposed a broccoli light-up aptamer-included 3WJ RNA scaffold (b-3WJ) integrated with a nucleic acid bioproduction and self-packaging system to produce b-3WJ packaged red-fluorescent Qβ VLPs (rQβ@b-3WJ), followed by the conjugation of TAT peptide on the surface (TrQβ@b-3WJ) to enhance cellular internalization for highly efficient gene silencing. The benefits of this system are as follows: (1) biological production of all components and self-packaging, (2) multigene silencing,