document: (BCA) Protein Assay Kit (Thermo). Proteins were electrophoresced using an 8% SDS-PAGE gel (approximately 20 μg per lane) and transferred to a polyvinylidene fluoride (PVDF) membrane. After blocking with blocking solution (5% milk, 0.1% Tween-20 in TBS buffer, pH=7.4), the beta-actin internal control was stained with beta-actin monoclonal antibody (CAT: 66009-1-Ig, Proteintech®, 1/10000 dilution), glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was stained with antiGAPDH antibody (clone 2D9.