document: Plasmid construction for Qβ VLPs and 3WJ RNA scaffolds. The QβCP expression vector pCDF-QβCP was constructed according to our previous study.46 The QβCP DNA sequence and mCherry red fluorescent protein DNA sequence were inserted into the pCDFDuet-1 plasmid. The mCherry DNA sequence was originally from plasmid pmCherry. After cloning into the pET28-b(+) plasmid using BamHI and NotI restriction enzymes for insertion, the mCherry sequence was cloned into pCDFDuet-1 to produce the plasmid