document: of 2 μM rQβ@b-3WJ in PBS buffer (pH=7.4) for 30 min at 25 °C in the dark and then purified using a filter column (Amicon Ultra15 Centrifugal Filter Units. 100,000 MWCO. Merck Millipore, LOT: R6EA45140, Ireland) with PBS buffer. The samples were desalted with a filter column (100,000 MWCO) and washed 3 times with PBS buffer. Subsequently, the maleimide-terminated rQβ@b-3WJ was reacted with 30 μL of Cys-TAT solution (0.3 mg/mL) at 25 °C for 2 h in the dark and then purified again using the