document: For RNAi therapeutics, the stability of RNA in biological condition is an important factor in determining its therapeutic efficiency. The RNA backbones with 2’-fluorine, 2’-O-methyl or 2’-amine modified U and C nucleotides are commonly used to render the RNAs resistant to RNase degradation or hydrolysis so far.42 However, the chemical process not only affects the biological functions of RNA molecules, but also contribute to higher production costs and lower yields. The presented technique in