document: 2b), representing the signal from individual Qβ@b-3WJsiSCR MG. All acquired fluorescence intensity time traces can be classified into three different patterns: one-step photobleaching with a probability of 76.2%, two-step photobleaching with a probability of 12.4% and no significant photobleaching during the acquisition time window with a probability of 11.4% (Figure 2c). Averaged intensity values of 6,872  1,892 and 3,889  1,000 were obtained for the first-step and second-step photobleaching