document: For in vivo Western blotting, the brain tumour tissues of mice treated with 5 μM rQβ@b-3WJsiEGFR Let-7g or TrQβ@b-3WJsiEGFR Let-7g were cut into small pieces and carefully washed in 3 mL PBS, then homogenized on ice by a Polytron blender in lysis buffer supplemented with a protease-inhibitor cocktail. The homogenates were centrifuged at 2,000 rpm for 10 min at 4 °C and the supernatant was assayed for total protein concentration by BCA Protein Assay Kit and stored at −80 °C until used for NF-κB