document: their further cleavage status with Dicer enzyme cannot be determined.43 At present, the Northern blotting method is commonly used to analyse the fragments from cleaved RNA by Dicer enzyme in cells, which is very complicated and time-consuming. But now, our designed light-up 3WJ RNA scaffold provides a convenient tool distinct from the Northern blotting for researchers to monitor the cleavage status of delivered RNA molecules with Dicer enzyme in real time by fluorescence microscopy.