document: was cloned into pCDFDuet-1 to produce the plasmid pCDFDuet-1-mCherry. The QβCP sequence was originally from pCDFDuet-1-QβCP-GFP, which has been described previously,26 and was inserted into the pCDFDuet-1-mCherry plasmid using EcoNI and the XhoI restriction site to produce the protein expression vector pCDFDuet-1-QβCP-mCherry.