document: In vivo antitumor efficiency of gene silencing-enhanced radiotherapy. All animal experiments were approved by the Animal Committee of Chang Gung University and adhered to the experimental animal care guidelines (IACUC NO. CGU106-036). Mice were raised in a room with a thermostat at 26°C. Nu/Nu mice weighing approximately 25–30 g (5–6 weeks old) were tested to confirm the efficacy of the proposed approach. Intracranial brain tumours were induced by transplantation of U87MG cells into mouse