document: Transwell migration assay. Transwell assays generally followed the Cell Migration, Chemotaxis and Invasion Assay using the Staining protocol (Corning, NY, USA). U87MG cells were treated with VLP-based samples (Qβ VLPs, TrQβ@b-3WJsiEGFR Let-7g) for 72 h. Then, the treated cells were harvested, resuspended in FBS-free DMEM and seeded at 2×104 cells in the insert of a 24-well Transwell plate. The reservoir volume was 0.65 mL of DMEM containing 10% FBS in each well. After 4 h of incubation, the