document: (10 mM) urea at 37 °C, the b-3WJsiEGFR siLUC within rQβ VLPs was approximately 80% intact. In contrast, less than 10% of naked b-3WJsiEGFR siLUC was intact under the same conditions (Figure S6a). Similarly, nearly 100% of rQβ VLPs packaged with b-3WJsiEGFR siLUC remained intact after 400 s at an RNase A concentration of 0.1 mg/mL, most likely because rQβ VLPs protect packaged b-3WJsiEGFR siLUC from degradation by nucleases, which are larger than the pore size of the rQβ VLPs.32 There was no