document: enzyme to specifically degrade the complementary mRNAs. However, Northern blotting is not only complicated and time-consuming but also cannot monitor the RNA cleavage status in real time. Otherwise, our designed b-3WJsiEGFR siLUC can react with DFHBI-1T to emit green fluorescence when its structure is intact, but the broccoli aptamer loses its reactivity with DFHBI-1T when the b-3WJsiEGFR siLUC is cleaved by Dicer enzyme, resulting in a decrease in green fluorescence. As proof, we treated the in