document: We also investigated the stability of naked b-3WJ RNA and packaged b-3WJ RNA after treatment with 10 mg/mL urea for 0, 1, 2, 4, 15, 24, 48, 72, 96, 120, and 144 h. Then, the fluorescence intensity was analysed to estimate the stability of naked b-3WJ RNA and packaged b-3WJ RNA at different time points by an M2 ELISA spectrometer.