document: Next, we verified the effectiveness of Qβ@b-3WJ in gene silencing by replacing siSCR with EGFR siRNA (siEGFR) and MG aptamer with luciferase siRNA (siLUC) in the 3WJ RNA scaffold, named rQβ@b-3WJsiEGFR siLUC (Figure 3a and Figure S2b). After IPTG induction and purification, the extracted rQβ@b-3WJsiEGFR siLUC showed a uniform spherical shape with a diameter of approximately 31.4 ± 0.5 nm, proving again that mCherry protein and b-3WJsiEGFR siLUC packaging do not affect the self-assembly of Qβ