document: Northern blot analysis. U87MG cells were seeded in 12-well plates (∼1 × 105 cells per well) and incubated for 24 h. The Qβ VLPs (1 μM) or TrQβ@b-3WJsiEGFR siLUC (1 μM) were then added to the culture medium. The cells were harvested after 24, 26, 36, and 48 h of incubation, and the small RNAs were extracted with the mirVana PARIS kit (Life Technologies, Carlsbad, CA, USA), resolved by denaturing gel electrophoresis (urea PAGE), transferred to a Hybond™-N+ membrane (GE Healthcare) by the capillary