document: To impart better biosafety to the nucleic acid drug and considering that most manufacturing methods usually use liposomes to encapsulate naked RNA (i.e., siRNA, miRNA, mRNA), we designed a biological RNA production and self-packaging system, including bacterial Qβ phage capsid, b-3WJ RNA scaffold production, and then automatic packaging. The b-3WJ RNA scaffold consists of the following parts: (1) bacterial Qβ phage capsid binding hairpin, (2) three functional regions junction together with a