document: 2d (iii)). For molecules exhibiting no significant photobleaching, an averaged intensity value of 3,838  1,400 was obtained, indicating the signal coming from a single DFHBI-1T/b-3WJ RNA complex in individual Qβ@b-3WJsiSCR MG (Figure 2d (iv)). Based on SMPB analysis, 87.6% of Qβ@b-3WJsiSCR MG contains one DFHBI-1T/b-3WJ RNA complex in one Qβ VLP, while 12.4% of Qβ@b-3WJsiSCR MG contains two DFHBI-1T/b-3WJ RNA complexes in one Qβ VLP.