document: those cells were blocked by 1% BSA solution for 20 min. Followed by incubation with EGFR antibody (1/1000 dilution, EGFR Rabbit Ab, CAT: A11351, ABClonal), or IKKα antibody (1/1000 dilution, IKKα Rabbit mAb, CAT:A19694, ABClonal), or NF-κB antibody (1/1000 dilution, NF-κB p65/RelA Rabbit mAb, CAT: A19653, ABClonal) for 1.5 h. The cells were further incubated with secondary antibody (1/500 dilution, 488-conjugated Goat Anti-Rabbit IgG (H+L), CAT: AS053, ABClonal) for 1 h. All samples were