document: In-gel urea page electrophoresis of RNA scaffolds. In vitro transcribed b-3WJ RNA scaffolds were prepared following the protocol of the HiScribe™ T7 High Yield RNA Synthesis Kit (NEB, USA). The packaged b-3WJ RNA scaffolds were prepared by extracting the b-3WJ RNA scaffolds from Qβ@b-3WJ according to our previously described methods.[1-2] Purified RNAs (1 μg/well) were electrophoresced with 8% urea page at 90 V for 4 h. After washing with DI-H2O, the gel was stained with DFHBI-1T to observe the