document: for the first-step and second-step photobleaching of individual Qβ@b-3WJsiSCR MG containing two DFHBI-1T/b-3WJ RNA complexes, respectively (Figure 2d (i)-(ii)). For individual Qβ@b-3WJsiSCR MG containing a single DFHBI-1T/b-3WJ RNA complex, an averaged intensity value of 4,360  773, similar to the value obtained in the second-step photobleaching of Qβ@b-3WJsiSCR MG containing two DFHBI-1T/b-3WJ RNA complexes, was obtained (Figure 2d (iii)). For molecules exhibiting no significant