document: three functional regions junction together with a three-way junction motif from bacteriophage Phi29 hexameric motor pRNA. Such structures help to assist RNA stabilization and facilitate cellular ingestion, resulting in therapeutic effects. To prove that the scaffold can be folded in the correct structure, we inserted scrambled siRNA (siSCR) at the left end, malachite green (MG) aptamer at the right end, and broccoli aptamer at the bottom of the scaffold, named b-3WJsiSCR MG (Figure 1a). The