document: In summary, we have successfully developed TrQβ@b-3WJsiEGFR Let-7g-based genetic therapeutics, which can efficiently knock down EGFR and IKKα simultaneously and inactivate NF-κB signalling, thereby inhibiting DNA repair in a highly efficient manner for enhancing radiotherapy. Impressively, the status of released b-3WJsiEGFR Let-7g processed into its mature form by Dicer for gene silencing can be easily and real-time monitored using fluorescence microscopy. TrQβ@b-3WJsiEGFR Let-7g showed a robust