document: was stained with antiGAPDH antibody (clone 2D9. CAT: TA802519, Invitrogen, 1/5000 dilution). Target protein EGFR was stained with EGFR antibody [GT133] (CAT: GTX628887, GeneTex, 1/2500 dilution), NF-κB (P65) was stained with NF-κB p65/RelA antibody (CAT: A19653, Abclonal, 1/1000 dilution), and luciferase was stained with antifirefly luciferase antibody [Luci17] (ab16466, Abcam, 1/1000 dilution). A goat antimouse IgG (H+L)-HRP antibody was used as the secondary antibody for all proteins