document: In vitro cell culture. The glioma cell Line U87MG was cultured in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 2.2 mg/mL sodium carbonate, 10% foetal bovine serum (FBS), 50 µg/mL gentamicin, 50 µg/mL penicillin, and 50 µg/mL streptomycin. Cells were harvested by 0.05% trypsin-ethyldiaminetetraacetic acid (EDTA) solution and washed with PBS buffer (pH=7.4) three times before seeding into experimental wells.