document: Broccoli aptamer tracking images in live cells. U87MG cells were seeded in a glass-based chamber (∼1 × 105 cells per well) and incubated for 24 h. TrQβ@b-3WJsiEGFR siLUC (1 μM) was then added and incubated for another 24 h. The medium was removed, and the cells were washed with PBS followed by incubation with DFHBI-1T-containing medium. The cells were imaged using 3D-Cell Explorer-Fluo microscopy (Nanolive) at 60X, and images were taken every 10 min for cell nuclei (blue colour), Qβ VLPs (red