document: Cell uptake of VLP-based samples by U87MG tumour spheroids. U87MG cells were cultured at 5×104 cells per well in U-end 96-well plates for 72 h to form a spheroid 3D culture. One micromolar VLP-based samples (rQβ@b-3WJsiEGFR siLUC or TrQβ@b-3WJsiEGFR siLUC) were added to the cells and incubated for another 24 h. The cell nuclei were stained with Hoechst 33342, and the b-3WJ RNA scaffolds were stained with DFHBI-1T followed by PBS wash. The distribution of Qβ VLPs and delivered b-3WJsiEGFR siLUC