document: in green fluorescence. As proof, we treated the in vitro transcribed b-3WJsiEGFR siLUC with a ShortCut® RNase III kit at 37 °C for 20 min, and the green fluorescence was significantly weakened compared to the green fluorescence of the non-treated b-3WJsiEGFR siLUC (Figure 4d), indicating that b-3WJsiEGFR siLUC can be cleaved into small fragments by the Dicer enzyme and lose its reactivity with DFHBI-1T. Subsequent incubation of TrQβ@b-3WJsiEGFR siLUC with U87MG cells in the presence of DFHBI-1T