document: Luciferase reporter assay. Luciferase-stable U87MG cells treated with 1 μM VLP-based samples (Qβ VLPs and TrQβ@b-3WJsiEGFR siLUC) for 72 h were harvested and washed with PBS (pH=7.4). The luciferase expression analysis process of luciferase-stable U87MG cells generally followed the Luciferase RGA high sensitivity, 200 assays (Roche) protocol. The chemiluminescence was detected by an M2 ELISA spectrometer.