document: 611 nm was obtained upon 473 nm excitation (Figure S5), indicating that the co-packaged mCherry protein would not interfere with the reactivity of broccoli aptamer with DFHBI-1T. However, the emission of DFHBI-1T shifted to 575 nm from 510 nm, most likely because the DFHBI-1T (donor) emission spectrum overlaps with the mCherry (acceptor) excitation spectrum to excite the packaged mCherry. The results indicated that the mCherry protein and b-3WJsiEGFR siLUC were successfully produced by E. coli