document: RNA scaffold fluorescence assay. Purified Qβ@b-3WJ was resuspended in RNA aptamer binding buffer (40 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) HEPES, 100 mM KCl, 1 mM MgCl2, pH=7.4), coincubated with 10 μM DFHBI-1T for 30 min at 37°C and subjected to UV-VIS spectrometry to measure the maximum absorbance wavelength as the fluorescence excitation wavelength. Fluorescence intensity measurement was performed using an M2 enzyme-linked immunosorbent assay (ELISA) spectrometer (Molecular