 Brown.
Defender Works V8 Trophy launch film here.
Both 90 and 110 wheelbase Defender Works V8 Trophy derivatives are now available to order direct from Land Rover Classic, with prices starting from £195,000 (about R4-million) for a 90 in the UK.
Oh yes this definitely happens and there's also a perception from outside that this happens too. People don't think that a dad can handle his own kids unless there is a woman to help him. Right after we got married my MIL asked when we were going to go to court so SD could live with us. What?! She acted like custody with something you just signed up for and that my DH had opted out of it but could go back and get full custody whenever he decided to go to court? That's not at all how it works and what did I have to do with him getting more custody?dove-wedding-card-dove-close-up Share this:Click to share on Facebook (Opens in new window)Click to share on Pinterest (Opens in new window)MoreClick to share on Reddit (Opens in new window)Click to share on Twitter (Opens in new window)Like this:Like Loading... Published by hydeparkhill Hello! My name is Eileen! I am a wife, mother, teacher, crafter, artist, chief cook and bottle washer! ahahhahaa! I have an active Etsy shop also named Hyde Park Hill. I enjoy creating in a variety of medium---paper, clay, and glass. Come along on my creative adventures and follow my blog! View all posts by hydeparkhillThis happened to my brother. He is naval special forces, was in Iraq (somewhere we weren’t allowed to know). His convoy was hit with an IED, his friends, his brothers in the humve that got hit all died. He said there was no cleanup crew so they literally had to spray the remains of his friends out of the humve so it could be stripped of the equipment. Second, this man (my brother) won the medal of Valor for carrying his CO who was shot out of the line of fire, he saw a group getting ready to fire an RPG at them so he shot and killed them.  It TORTURED him when he got back. He didn’t even tell us about getting the medal of Valor and didn’t want anyone at the pinning ceremony (a member of my brothers team called my dad and told us about it).  He had just lived through an actual nightmare, clearly had PTSD AND finds out his wife was cheating on him.  You really want to pick a fight with a navy seal whose team was living with him to support him?  I was just a teenager at the time and he lived across the country but I can’t imagine what it was like for him to deal with all of this. The guy she was cheating with was dishonorably discharged.

My brother has since moved on, is doing amazing now. Remarried to a wonderful woman, has full custody of his first kid and has another child with his new wife. They are an amazing family and he got his happily ever after.The pen is awesome! Writes so smooth and looks very professional!
It arrived earlier than expected and they look amazing. I had my doubts because they are not that expensive as other ones that I had looked at but everything was great. the inks glides perfectly on the paper and it looks so cute.
The artwork is great, I have not handed out the pens yet, so I do not have any feedback on the quality.
I am extremely happy with the pens we received. Our organizational information is clearly legible. Thank you so much and I look forward to future orders.
Our logo looks great! I like the message of this product, the presentation, the quality, and the price! I was happy to find something to give to my staff for Staff Appreciation Day this year.
Very nice. Dependable and durable. Very popular.A context-specific circadian clock in adipocyte precursor cells modulates adipogenesis

ABSTRACT The circadian clock is an intricate molecular network that paces a variety of physiological process to ~ 24 hour day/night cycles. Whereas the central circadian clock in the brain is primarily entrained by light signals, peripheral circadian clocks, which are in most cells in the body, receive cues not only from the central pacemaker but also endocrine and other systemic and tissue-specific signals. Prior studies have connected peripheral circadian clocks to metabolism, primarily with studies focused on the robust clock in the liver that responds to feeding/fasting cycles. Adipose tissue is also critical for metabolism and adipocytes have circadian clocks. Yet, the role of the circadian clock in adipocytes is poorly understood. Here we describe our studies that revealed components of the circadian clock in primary adipocyte precursor cells (APCs) in mice. We made the surprising discovery of a particularly prominent role for the circadian gene Period 3 (Per3) in the APC clock. Furthermore, we elucidated that Per3 directly regulates an output pathway of the APC clock to modulate the expression of the Kruppel-like factor 15 (Klf15) gene. Finally, we discovered that this clock-Klf15 pathway regulates adipogenesis in APCs. These finding have important implications for our understanding of adipose tissue biology and metabolism and, we speculate, will generate opportunities to develop novel therapeutic strategies based on the context-specific features of the circadian clock in APCs.


Introduction
A circadian clock is an endogenous 24-hr-cycle timekeeping system, controlled by oscillations in molecular components. 1,2 The core circadian clock machinery uses positive feedback components (i.e. Clock and Bmal1) and negative feedback components (i.e. Per genes), to regulate the periodicity of circadian and clock-controlled genes. 1 Although, three Period paralogs (Per 1-3) are reported in mammals, Per3 is the least understood and was not regarded as a core circadian clock gene because deleting Per3 had minimal effect on circadian locomotion in mice. 3,4 The central circadian clock is located in the suprachiasmatic nuclei of the brain and most cells in the body appear to contain peripheral circadian clocks. 2,5 Interestingly, there are context specific differences in the components used in these peripheral clocks 6 but the implications and mechanisms driving theses differences are incompletely understood. Signals from photoreceptors in the retina to the central clock are generated by diurnal light cycles and entrain the central clock. 2,5 The central clock serves as an important pacemaker for peripheral circadian clocks. A number of functions of peripheral tissues are modulated by central clock pacing including: insulin secretion from the pancreas and sensitivity in muscle. 7,8 However, peripheral clocks are also subject to distinct inputs and contextual regulation that may uncouple oscillations from the central clock. 1,[9][10][11][12] For example, the timing of food intake can alter peripheral clock oscillations and rhythmicity of bile synthesis in liver. [13][14][15][16] These, and a body of other related studies, indicate that peripheral circadian clocks provide a mechanism that links peripheral tissues with systemic metabolism. [17][18][19][20] Another example of a metabolically relevant biological process that is influenced by peripheral circadian clocks is adipogenesis-a differentiation pathway where adipocyte precursor cells develop into mature adipocytes. [21][22][23] Dysregulated adipogenesis contributes to expansion of adipocyte tissue and thus is often implicated in obesity. 21 Furthermore, disruption of circadian rhythms is a riskfactor for obesity. 1,24 To begin to exam the mechanisms by which the circadian clock is connected to adipogenesis, previous studies employed in vitro adipocyte differentiation protocols using mouse embryonic fibroblasts cells (MEFs) and immortalized 3T3-L1 cells, e.g.. 25 Recently, we performed studies using primary adipocyte precursor CONTACT Brian J. Feldman feldman@stanford.edu cells (APCs) and approaches that monitor adipogenesis in vivo to establish the presence of peripheral clocks in bona fide APCs and mechanisms by which the circadian clock regulates the process of adipogenesis. 23

Results
As defined by a Sca1 (stem cell marker) positive and lineage marker negative population, 26 we isolated primary APCs from subcutaneous adipose depots of circadian clock reporter mice (mPer2 Luc ). 27 These studies demonstrated that primary APCs contain a circadian clock. 23 However, to our surprise, our further investigations elucidated that expression of the classic core clock component Per1 does not significantly oscillate in APCs. 23 Strikingly, we discovered that Per3, has the most robust circadian expression profile of the Per paralogs in primary APCs. 23 These findings suggested to us that Per3 might have a particularly prominent role in the context of APC biology. Indeed, deletion of the Per3 gene altered the circadian cycling of core clock components, supporting our hypothesis that Per3 activity impacts the clock machinery in APCs. 23 Intrigued by the above results, we investigated the functional role of Per3 in APC biology. To probe this question, we compared the level of adipogenesis occurring in Per3 deficient mice (Per3 −/-) in vivo to that of wild type mice using a pulse-chase approach we had previously validated. 23,28,29 Using this protocol, we revealed that Per3 −/mice have increased levels of adipogenesis in vivo. 23 The increased adipogenesis upon Per3 ablation was also captured in ex vivo culture assays by monitoring the levels of spontaneous adipogenesis of isolated primary APCs, indicating that disruption of Per3 in APCs was sufficient to cell autonomously promote adipogenesis in APCs. 23 Next, to elucidate the mechanisms by which Per3 regulates adipogenesis in APCs, we performed unbiased genome-wide expression profiling studies comparing APCs isolated