 slightly above the rest, "Bulletproof" and "I Blame You" for instance, the music does inspire that familiar feeling of wanting to bang your head or jump around. I guess it's another notch in the belt of Canadian quality, and it forces me to realize, that this band still won't be the one I need as an excuse to bury this genre of music all together in a review. Sad for me and my elitist beliefs, but good for Evans Blue and any casual appreciator of the nu-metal/post-grunge sound. Check out the two mentioned bands first though, and then if you still want more, then there's no reason not to get "Evans Blue".
Now 38 years old and clean for for awhile now, Lamar Odom now is plotting his return to basketball, but not with the Lakers. Inspired by Stephon Marbury, he is reportedly going to play in China. He has been working out and looks good. Hit this twitter link to see a workout Video.... 1987631104
It is sad that drugs have ruined so many lives. Lamar missed out while he was on his prime of an nba career because of drugs. But he is not the only one, just look around and you see it every where. Good families have been destroyed because of a family member that can not break away from using them. Some go so far that they end up DEAD.
I'd like to see him in the BIG 3.
Drugs are cheaper in China. He'll enjoy it
He can get some Asian eyebrows tattooed on while he's there lol
Yeah that’s what I was thinking as well. Just need to keep him away from Snoop. Lol.
Yeah, the Big 3 would be a good match, but it is like almost over isn't it? And I think he will probably make more money in China.
And Cocaine....lmao.
And brothelsFood
freezing serrano peppers
I bought way more serrano peppers at the farm market today than anyone could use in a week! But they were so pretty and I couldn't resist.
Think I can chop and freeze them? What would be the best way? Ice cube trays with water? Or could I just chop them and stick them in freezer bags?
Talk is closed, but that doesn't mean the conversations have to stop!
Check out this post for a more thorough explanation, and jump onto our Facebook page or our Twitter feed to keep the conversations going!Lead Exposure Induced Neural Stem Cells Death via Notch Signaling Pathway and Gut-Brain Axis

Numerous studies have examined the effects of lead (Pb) on cognitive ability. It is essential for the brain to maintain its functions through the differentiation of neural stem cells into various types of cells. Despite this, it remains unclear how Pb exposure affects neural stem cells and how it does, so the Pb-exposed mice were treated with the Notch inhibitor DAPT after we established the Pb exposure models. Neuronal stem cells and autophagy were assessed by immunofluorescence staining and western blot. The microbiota of the feces was also analyzed using the 16S rRNA amplicon sequencing technique. In this study, we found that Pb exposure caused neural injuries and deficits in neural stem cells, whereas DAPT rescued the damage. With DAPT, Pb-induced autophagy was partially reversed. Exposure to Pb also reduced inflammation and damaged gut barrier function. Furthermore, Pb exposure led to low bacterial diversity, an increase in pathogen abundance, and an unusual mode of interaction. Taken together, this study revealed that damages in neural stem cells contributed largely to cognitive impairment during Pb exposure, and this process was partially dependent on the Notch pathway and gut dysbiosis.


Introduction
As one of the most widely known heavy metals, lead (Pb) is recognized as an important pollution source for air, water, and soil. In addition to damage problems in the nervous system, Pb also cause problems in cardiovascular system, hematological system, and kidneys. Pb exposure has a great impact on cognitive and behavioral development. Accumulating studies showed that Pb exposure increases the risk of major depressive disorder, anxiety disorder, and panic disorder [1]. The workers exposed to Pb often showed impaired attention, speed, working memory, visuospatial abilities, and general intellectual performance. Childhood with high Pb exposure level are at higher risk of lower Intelligence Quotient (IQ) scores and higher risk of behavior problems, including hyperactivity, inattention, and antisocial behavior [2]. More importantly, it was also found that Pb exposure during childhood is linked with long-term negative effects on psychological well-being and personality in adulthood [3,4]. Pb exposure in the environment continues to be a serious public health problem.
Stem cells are neuronal precursors that differentiate into various types of cells, including neurons, oligodendrocytes and astrocytes. In the event that this homeostasis is disrupted, numerous defects can appear, such as defects in nervous system development, motor disturbance, and cognitive symptoms. As previously reported, in zebrafish, Pb exposure caused impaired neurogenesis and abnormal apoptosis in the neuronal cells. In a rat model exposed to Pb, it was reported that the neuronal stem cells are less likely to survive and proliferate. Furthermore, Pb is also shown to reduce the proliferation and viability of the neuronal progenitor cells [5].
Development and patterning of neuron stem cells are controlled by a complex regulatory network, which is affected by various signaling pathways, including Notch, Wnt signaling, and receptor tyrosine kinase. They affect the determination of cell fate.
Notch signaling is crucial for brain development, not only for stem cell proliferation but also for neuronal differentiation, and for keeping neural precursors undifferentiated and inhibiting neural differentiation. However, despite stem cells and Notch pathways playing key roles in the nervous system, their cross-talk and roles in Pb exposure-induced cognitive deficit remain largely unexplored.
Accumulating evidence from human and animal studies suggests that environmental pollutants can alter gut microbiota, disrupting physiological homeostasis, partly contributing to the development of nervous system diseases. The commensal bacteria are sensitive to heavy metals. Cadmium can cause gut damage and dysbiosis, and some recent studies showed that Pb exposure also affects the digestive system and intestinal microbiota in birds. Furthermore, probiotics (Bacillus coagulans R11) are considered useful tools for removing lead ions from the gut and protecting the gut against damage due to villitis [6]. It has been reported that Notch signaling can regulate the differentiation of secretory cells and absorptive cells in the gut, which may shape the composition of mucus-associated bacteria [7].
In this research work, the effect of Pb exposure on cognitive ability in mice was investigated. Subsequently, it was explored whether Notch signaling is involved in this process. This study demonstrated that the Notch pathway in vivo regulated the neural stem cells. It was also discovered that Pb induces elevated autophagy and this process can be restored by DAPT. Additionally, this study shed light on the peripheral factors that contribute to the negative effects of Pb exposure on cognitive performance, and may provide clues as to how Pb-related cognitive impairments may be treated.

Ethics Statement.
A committee of the Institute of the Fourth Military Medical University approved this study (No. IACUC-20190950) and throughout all experiments involving animals, the National Institutes of Health Guide for the Care and Use of Laboratory Animals was followed.

Animals.
These studies utilized mice aged 3 weeks (C57BL/6). The mice were kept in a temperaturecontrolled environment (22°C). On day 3, each of the mice were randomly assigned to one groups, including the control group, the low-dose Pb group, and the high-dose Pb group. In deionized water, Pb acetate was dissolved at various concentrations (0, 100 ppm, and 300 ppm). Model was constructed using the drinking water method and the three group-control, low-dose Pb exposure group, and high-dose Pb exposure group-were given deionized water that con-tained lead acetate. The exposure lasted for eight weeks. Additionally, all mice in the three groups were always provided with food. After 8 weeks of exposure to Pb, behavioral tests were conducted. In accordance with the "Guide for the Care and Use of Laboratory Animals of the National Institute of Health", the study was conducted.
2.3. Pb Concentration Analysis. The left ventricles of mice were used for collecting blood samples (600 μl) for analysis of Pb levels in the blood. Measurements of Pb levels were carried out by a graphic furnace and an atomic absorption spectrometer (AAS).

Morris Water
Maze. The test was performed as previously reported [8]. Our experiments were conducted in a tank containing opacified water of 120 cm diameter and 50 cm depth. An above-water video camera monitored the mice from a platform with a diameter of 10 cm. The trial involved placing a mouse in the water at one starting point. Animals that had difficulty finding the platform within two minutes were guided to it, where they stayed for 10 seconds. A 120-second swim was allowed after the hidden platform test without any restrictions on the mice. Swim speed, escape latency, and distance traveled were measured during each trial. Each quadrant's entries, swimming speed, and time were recorded with cameras. In order to analyze these data, we used Noldus Ethovision XT (Wageningen, Netherlands).

Cue and Contextual Fear
Conditioning. The test was conducted using the NIR Video Fear Conditioning System for mice (Med Associates, Inc., St. Albans, VT, USA). After a 2-min exploration time, we performed two pairs of conditioned-unconditioned stimuli pairings (white noise, foot shock intensity, duration of 30 seconds, and footshock intensity). 24 hours after the training, we conducted context tests. After six minutes of training, we conducted cue