 your legacy.
This presentation will be beneficial to those who are curious about estate planning and those who want to know more about including charity in your plans.
At the dark end of the rainbow: data gaps in tattoo toxicology

Toxicology and consumer safety have significantly improved since the beginning of the last century. In current times, thorough testing of goods is being premised in our society. Yet this trust might be questioned with regard to the colorful inks used in tattooing or permanent makeup.

occurring they seem manageable by improved hygienic standards. On the other hand, allergic side effects may have a major impact on the health of consumers (Kluger 2015;Brady et al. 2015;Wenzel et al. 2013). Since the ink is being administered into deeper layers of the skin, the obvious and only effective therapeutic counteraction, an immediate removal of the respective allergen remains unfeasible in the case of tattooed skin. While consumers affected by cosmetics can easily remove and avoid the allergen, tattooed individuals are unable to terminate the exposure right after the onset of symptoms (redness, swelling, itching, inflammation, etc.). For sure, this difference triggers one of the major health concerns and actually points to an inept equalization of tattoo inks with cosmetics. Even the method of laser-mediated pigment removal is usually to be refused when allergic reactions become apparent since the procedure might further mobilize ink particles via fragmentation. In some cases, it even may promote the formation of the actual allergenic species (Bernstein 2007). As a last resort and ultima ratio, in the case of severe allergic side effects only invasive surgery including transplantation seems applicable if anti-inflammatory treatment fails to limit the damage and to calm down the reaction.
The mostly feared and discussed risk factor for tattooing is the supposably carcinogenic potential of the inks applied. However, available studies that evaluated the association between skin (and other types of) cancer and the presence of tattoos failed to reveal any connection due to an extremely low number of cases (Kluger and Koljonen 2012). As a great disadvantage, past cohort and case-control studies did not include tattooing as possible risk factor although theoretically suited to link or to reject any connection between tattoos and cancer. Irrespective of the lack of epidemiological data, genotoxic and carcinogenic primary aromatic amines (pAAs) or polycyclic aromatic Toxicology and consumer safety have significantly improved since the beginning of the last century. In current times, thorough testing of goods is being premised in our society. Yet this trust might be questioned with regard to the colorful inks used in tattooing or permanent makeup.
The revival of tattooing in Europe during the last century arose from sailors traveling the world and linking the image of "the daring" to tattooed individuals (Laux et al. 2016). In addition, apparent risks from poor tattoo equipment and adventurous inks mixed from ashes, red bricks, plant leaves and varnishes disappeared as a result of the professionalization of the tattoo industry. A closer look on the history of legislation in this field reveals the remaining pain points of tattoo safety though. Due to the lack of toxicological data, currently safety assessment is mainly deduced from cosmetic ingredients. As a consequence, tattoo legislation in Europe is still based on an exposure scenario placing the product on top of the skin, rather than right into the middle of living tissue beneath the epidermal skin barrier. Certainly, the presence of blood vessels and neurons considerably affects both the bioavailability of pigments, auxiliary ingredients and contaminants on the one hand, as well as their tissue and cellular interactions on the other. Although the limited regulation of tattoo inks is now recognized for some time, the putatively low prevalence of severe side effects has rather led to a "show-me-the-dead-bodies" mind set in the general public and of healthcare officials alike.
Indeed, microbiological outbreaks in Europe are rare (Drage et al. 2010). Further, although still occasionally hydrocarbons (PAHs) are frequently detected as impurities of tattoo inks. Due to their bioavailability, they can travel across the body and its organs and tissues, including those more prone to be affected by these compounds (i.e., target tissues). For sure, the analytical detection of genotoxic carcinogens in tattoo ink formulations that are supposed to be injected into skin tissue well supplied with blood, this fact alone might already trigger serious concerns.
Even when produced in a highly pure manner, tattoo pigments still bare the risk of getting fragmented or converted by ultraviolet and visible light, laser irradiation and/ or cellular metabolism. To adequately address possible long-term (chronic) health effects, ingredients of tattoo inks in their original form as well as descendants abiotically or biotically formed over time within the human body need to be considered and seriously assessed (Laux et al. 2016). Already today, many of the hazardous substances present in tattoo inks or originating over time from certain ingredients are toxicologically characterized (e.g., pAAs, PAHs).
Besides hazard identification, the evaluation of compoundspecific biokinetics and internal target tissue exposure levels are of utmost importance to reliably assess the longterm risks associated with this increasingly popular form of body modification.
In the present issue of "Archives of Toxicology," we propose to employ pyrolysis-mediated fragmentation followed by gas chromatographic separation and mass spectrometric detection as highly sensitive method to identify hazardous substances that are likely to emerge through tattoo pigment decomposition during sunlight exposure or laser irradiation. The types of pigments applied have become famous for their high color brilliance, and due to their low purchasing costs impressively bright and colorful skin modifications become more common and pervasively visible in the mainstream. The data collected in our study reveal that most organic pigments used in tattoo inks are thermically cleavable to yield potentially harmful substances. In particular, azo pigments might be decomposed into pAAs that can make up to 20 % of the total peak area found in the corresponding pyrograms. The formation of genotoxic and carcinogenic pAAs has already been reported after sunlight and laser irradiation of azo pigments (Engel et al. 2010). Compared to azo compounds, other more lightfast pigments like quinacridones, diketopyrrolo-pyrrols and phthalocyanines revealed less decomposition products when applying similar temperatures. Nonetheless, we found that essentially all organic tattoo pigments can be cleaved into toxins such as benzene, cyanides and others, upon administration of sufficient amounts of thermal energy. On the other side, such heavy decomposition is as yet not described in the literature to occur upon sunlight exposure. Yet own work has shown laser irradiation being capable of cleaving copper phthalocyanine blue pigments into the same pattern of fragments as found in pyrolysis at 800 °C (Schreiver et al. 2015). With this, we proved artificial pyrolysis as suitable method to predict the identity of descendants that emerge upon both thermal and photochemical pigment cleavage.
Taking into account that-at average-about 2.5 mg pigments will be deposited inside the skin per square centimeter (Engel et al. 2008) while creating an upper arm tattoo of 400 cm 2, an individual body load of 1 g of particles can be calculated (Fig. 1). Highly tattooed people may even carry up to 40 g of pigment within their body. Size, color and particle density of the respective tattoo will thus determine the amount of possible harmful substances as well as the portion of hazardous decomposition products that chronically affect skin and lymph nodes (Anderson et al. 1996). Future investigations are urgently required to assess the quantities of the proposed compounds evolving from the pigments upon laser treatment or during chronic sunlight exposure. These data can then be used to describe possible worst-case scenarios and to reliably assess the health risks that might be associated therewith. Certainly, the great variety of pigments and other ingredients used in ink formulations will make it rather a long way to go to clear the dark end of the tattoo rainbow-eventually.
Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http:// creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.Somewhere over the rainbow exists a land of make believe. Follow the Yellow Brick Road and join Dorothy™, Tin Man™, Scarecrow™ and Cowardly Lion™ for an exciting adventure in the Land of Oz.
THE WIZARD OF OZ™, one of the most popular land-based casino themes of all time, is now available online! This exciting theme that kick-started the legendary series of games in the Land of Oz features exciting random WILD reels, courtesy of Glinda the Good Witch™, which could randomly fill your screen with WILD reels on any base game spin. Players also have the chance to enter the Emerald City™ picking bonus where treasures await and lucky picks could earn players an audience with the Great and Powerful Oz himself! There's also the thrilling Haunted Forest free spin feature where Winged Monkeys could add WILD symbols to any spin for an increase chance at line pays.Bush vs Gore: The Endless Election
CNN will air a special report, Bush vs. Gore: The Endless Election, hosted by CNN’s chief political analyst Gloria Borger on November 2 at 9pm ET in a “retelling of election 2000,” 15 years after it happened.
Via the press release…
It was an election night that ended with epic confusion as networks called the key swing state of Florida for both Gore and Bush – and the uncertainty soon escalated into 36 days of dramatic combat for