 length of my text in column 2 is greater than 10<br>
  `Filter data on any column using simple expressions` with `len(c2)<=10`
- Create new rows for every comma separated value in column 3; Unfolding<br>
  `Unfold columns from a table` with `Column 3` and `Comma`
- Split the first four characters of a line into it's own column<br>
  `Replace Text in entire line` with `Find Pattern`: ^(.{4}) and `Replace Pattern`: &\t
- Add the basepairs "TA" to the end of each sequences<br>
  `FASTA to Tabular` → `Add column` with `TA` → `Merge Columns` → `Cut columns` → `Tabular to FASTA`
- Add a quotation mark to every row<br>
  `Compute an expression on every row` with `chr(34)` (34 is the [ASCII](http://www.asciitable.com/) code for `"`)
- Count all columns with numbers that do not contain 0. Usefull if you want to calculate the mean but want to exclude all columns that are 0.<br>
  `Compute an expression on every row` with `bool(c1) + bool(c1) + bool(c3)`... 
- Calculate log2 (not log10) from a column (e.g. c1) adding a new column
  `Compute an expression on every row` with `log(c1,2)`


## HTS
- Map RNA-seq data<br>
  `HISAT` or `TopHat`
- Map DNA-seq data<br>
  `Bowtie` or `BWA`
- Map methylC-seq data<br>
  `Bismark`
- Downsample BAM/SAM files<br>
  `BAM/SAM Mapping Stats` will give you the number of reads/read pair in your
  BAM file in case you don't know it already. Then you just divide the number of reads
  you want to downscale to with the number of reads you have and use this
  fraction as the probability in `Picard – Downsample SAM/BAM`.
- Get all genes that are covert by reads<br>
  `htseq-count` with a gene annotation [GTF file](http://www.ensembl.org/info/website/upload/gff.html) on your BAM file  → `Filter data on any column using simple expressions` with `c2>0`
- Extract sequences from intercal files, like gff, bed, gtf. Returning FASTA file →<br>
  `Extract Genomic DNA using coordinates from assembled/unassembled genomes`

## Workflows
- Find two genes located nearby<br>
  [Description](https://github.com/bgruening/galaxytools/tree/master/workflows/ncbi_blast_plus/find_genes_located_nearby) :: [Tool Shed](https://toolshed.g2.bx.psu.edu/view/bgruening/find_genes_located_nearby_workflow)


## More Resources
 - Galaxy 101 - a must read for all HTS Padawan: https://wiki.galaxyproject.org/Learn/GalaxyNGS110
 - A lot of videos to learn using Galaxy while eating popcorn: https://vimeo.com/galaxyproject

## Disclaimer
All tools mentioned here are available from the Galaxy Tool Shed. Kindly ask your Galaxy Administrator to get access to them.
Edward Yang's soulful, glacially paced, and all out beautifully made coming-of-age story with a bitter-sweet fragrance although not quite on the level of his magnum opus -- A Brighter Summer Day--, is not far from it and definelty my favorite work of his from an enjoyment perspective. I loved how it just shows us the lives of the characters from such an observant point-of-view; never judging, and always interested in every character no matter their age. The performances playing said characters are top-tier in every damn scene, and in all moments raging from the profoundest of sadnesses, to the most innocent of conversations ("you can't see it by yourself, so I'm helping you").
I'm sure it could easily come off as boring to the impatient, but give it the time it deserves, and I assure you it would be one a most awarding experience.
One of the best films I've ever encountered, seriously.
Editor:
Last week I helped transport some of the last horses off of the Rockin River Ranch down Salt Mine Road in Camp Verde.
Arizona State Parks had decided all horses must go off this iconic ranch that has been a horse boarding and breeding facility for well over a quarter of a century.
Thank you Nature Conservancy and Arizona State Parks for destroying the culture and heritage of this famous old ranch.
Since it has been in the possession of State Parks, the beautiful green horse pastures are dying because the state is not irrigating them in this time of extreme drought, which I assume is their plan since they intend to turn these green pastures into RV parking sites.
Had this ranch been able to stay in private ownership it would have had owners that cared about the history and culture of this land much better than our so called “public land managers and their enviro group allies.”
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