 Although the other characters did not get much development, which I need to ignore since harem series tend to not do much of that anyway. It was still enjoyable for the comedic parts and everything else that has come into play in this episode. This series is really enjoyable, which is why I like the manga.  Sure that might have sounded a little biais, but it is true. I enjoy it since I enjoyed the manga. People who did not read the manga have been enjoying it so far too, so I do not feel as bad saying this as well.
As always I am curious on what you think of the episode. Yay Mero-sama is now in, next episode Rachnera will make a brief appearance at the end more than likely. Lala has also been confirmed. Anyway enough about future characters. As always I hope you enjoyed.
“Like I said I enjoy all the female characters.” – Is there anything else to enjoy from this show?
I’m going to be bias here because Mero is best girl, but this episode is very enjoyable. The lewdness at the beginning was totally unexpected. Story wise, it gets somewhat annoying when after 5 episodes the anime is still at introducing characters phase, but it’s not like there going to be much story anyway. At least that’s what I feel.
Mero’s tragedy fetish just makes me like her even more. 20/10 best monster girl.
Yeah, in anime form it does not work as well, since in the manga at first it takes about 15 chapters for the main girls to be introduced, This will more than likely translate into the first 7 episodes. So it is a little unfortunate at that end. At times because they introduce the girls left and right a part of me feels that it is somehow making fun of the harem genre or giving the bare minimum since the main plot for the first 30 chapters is who is he going to marry. Even then that is not really focused on but is a subject that shows up from time to time.
I haven’t read the manga. Is it good? I can’t really imagine reading a manga where ecchi is kind of the main focus.
The anime follows the manga super closely. I used to do a comparison for the manga and anime, but the anime follows it too closely so I found it pointless. The manga is a bit more ecchi at times since the show has taken the liberty of censoring stuff by some sort of means other than the light beams, fog, ect. The manga does not do that. But yes, obviously I will say the manga is good since I have been reading it for 2 years now and have all the volumes, normally ecchi series bother me but somehow this one doesn’t. If anything don’t bother reading one of the chapters and just flip through the pages before you think of reading it. Since looking at the images will probably give you an idea on how ecchi it can be at times. If it seems like it is not for you, probably sticking to the anime would be best since the ecchi-ness has been toned down a little bit.PostgreSQL is a popular database used by many organizations and database developers. Sometimes you may get an error saying ‘Fatal: Role Does not Exist’. This error can prevent you from executing most PostgreSQL commands that involve creating or modifying your data. In this article, we will learn how to fix this issue.
One of the simple ways to fix this problem is to login using postgres database user and then run the command where you get the error.
For example, let us say you got this error when you logged into PostgreSQL as test_user user and ran the following command.
Now login to PostgreSQL database using postgres user. You will be asked for password, enter it to login.
Then try running the above ‘create database’ command again. It should work now. Once you are done performing required operations, quit PostgreSQL by entering exit command.
If the above approach does not work, try creating your owner user test_user as postgres with sudo.
If this method also does not work, assign superuser role to your user using the following commands. Login to PostgreSQL as postgres administrator user.
Create superuser role for your user test_user.
Then create user.
Assign root privilege to user.
Enable login for that user, so you can run your commands from terminal.
In this article, we have learnt how to fix ‘fatal role does not exist’ error in PostgreSQL.
PostgreSQL fatal role. permalink.Cigarette smoke condensate attenuates phorbol ester-mediated neutrophil extracellular trap formation

Background Neutrophil extracellular traps (NETs) constitute a network of chromatin fibres containing histone and antimicrobial peptides that are released by activated neutrophils. NETs protect the host against infection by trapping and facilitating phagocytosis of potentially harmful pathogens. Objectives The aim of the current study was to investigate the effects of cigarette smoke condensate (CSC) on phorbol-ester (PMA)-mediated NETosis in vitro. Methods Isolated human blood neutrophils were exposed to PMA (6.25 ng/ml) in the presence or absence of CSC (40–80 µg/ml) for 90 min at 37oC. NET formation was measured using a spectrofluorimetric procedure to detect extracellular DNA and fluorescence microscopy was used to visualize nets. Oxygen consumption by PMA-activated neutrophils was measured using an oxygen sensitive electrode. Results Activation of neutrophils with PMA was associated with induction of NETosis that was significantly attenuated in the presence of CSC (40 and 80 µg/ml), with mean fluorescence intensities of 65% and 66% of that observed with untreated cells, respectively, and confirmed by fluorescence microscopy. The rate and magnitude of oxygen consumption by activated neutrophils pre-treated with CSC (80 µg/ml) was significantly less than that observed with untreated cells (73% of the control system), indicative of decreased production of reactive oxidants in the presence of CSC. Conclusion The inhibition of NETosis observed in the presence of CSC correlated with attenuation of oxygen consumption by PMA-activated neutrophils suggesting a mechanistic relationship between these events. If operative in vivo, smoking-related attenuation of NETosis may impair host immune responses and increase the risk of respiratory infections.


Introduction
Neutrophil extracellular trap formation (NETosis) is a recently described host defence mechanism characterised by the decondensation of chromatin and nuclear segmentation resulting in the extracellular release of netlike structures [1][2][3]. NET formation can be triggered by a number of physiological and non-physiological activators such as interleukin-8 (IL-8), lipopolysaccharide (LPS), phorbol esters, interferon-γ (IFN-γ), various types of bacteria and their products, viruses, fungi and the complement cleavage component, C5a. The phorbol ester, phorbol-12-myristate-13-acetate (PMA) activates NE-Tosis in vitro 4,5. PMA-induced NET formation requires protein kinase C (PKC)-mediated activation of the membrane-associated electron-transport, superoxide-generating complex, NADPH oxidase, as well as the activation of the enzyme peptidyl arginine deiminase 4 (PAD4) which leads to the citrullination of histones 6. This is fol-African Health Sciences @ 2017 Bokaba et al; licensee African Health Sciences. This is an Open Access article distributed under the termsof the Creative commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
African Health Sciences Vol 17 Issue 3, September, 2017 lowed by the release of decondensed chromatin web-like fibres entangled with citrullinated histones and impregnated with anti-microbial peptides and proteins including neutrophil elastase and myeloperoxidase 3,[7][8][9][10]. Activation of NADPH oxidase is apparently a critical requirement for NETosis since patients with chronic granulomatous disease (CGD) who are unable to generate reactive oxidants, do not produce NETs when activated with PMA. Furthermore, diphenyleneiodonium chloride, an inhibitor of NADPH-oxidase activity, when added to neutrophils effectively attenuates PMA-mediated NET formation 11,12. NET formation protects the host against infection which is achieved via the capture of microbes and viruses in the net-like structures of decondensed chromatin fibres which are impregnated with anti-microbial agents resulting in the immobilisation, localisation and possibly elimination of the infection 10,12. In this setting, NET formation is potentially an advantageous host defence mechanism.
Cigarette smoking is a common lifestyle habit worldwide, with 21% of the population older than 15 years, smoking tobacco on a regular basis 13. Cigarette smoke is composed of chemical compounds that include tar, nicotine, carbon monoxide, polycyclic aromatic hydrocarbons and high concentrations of oxidants and free radicals 14. Cigarette smoke has been shown to increase oxidant production by neutrophils 14,15, and to promote oxidative processes such as lipid peroxidation, protein carbonylation, thiol peroxidation and DNA oxidation. Cigarette smoking also recruits macrophages and neutrophils to the lungs and increases the citrullination of proteins [16][17][18][19][20]. To our knowledge, there are no publications to date evaluating the effects of cigarette smoke and cigarette smoke condensate on NETosis. In the current study, we investigated the effects of exposure of isolated human blood neutrophils to cigarette smoke condensate (a surrogate for cigarette smoke) on PMA-induced NET formation in vitro.

Ethics approval
The study was approved by the Research