Statistical analysis of glucose production from Eucalyptus pellita with individual control of chemical constituents
To understand the effects of the biomass constituents for enzymatic hydrolysis, a tropical hardwood, Eucalyptus pellita, was employed to change the content of chemical constituents and regression assessment was progressed. The liquid hot water and acid-chlorite pretreatment controlled hemicellulose and lignin removal rate from 3.1 to 91.7% and from 4.4 to 99.1%, respectively. Total lignin in E. pellita (34.8%) was obviously reduced up to 9.0% under 4 g of sodium chlorite and 0.8 mL of acetic acid with 3 times loading. In this case, acid-insoluble lignin (2.4%) was entirely eliminated compared with that of E. pellita (29.0%), whereas acid-soluble lignin (6.6%) was preferably increased compared with of E. pellita (2.3%). The maximum glucose yield (87.5%) after the combined pretreatment and enzymatic hydrolysis were dramatically improved compared with that of liquid hot water pretreatment alone (28.2%). Meanwhile, a high glucose yield (83.9%) could also be obtained by acid-chlorite pretreatment alone. The adjusted R-squared value of hemicellulose and lignin removal rate (0.9285) and lignin removal rate alone (0.9063) with glucose yield was determined by linear regression analysis. (C) 2019 Published by Elsevier Ltd.
Keywords:Liquid hot water pretreatment;Acid-chlorite pretreatment;Glucose production;Linear regression analysis;Eucalyptus pellita