The molecular basis of partitioning in aqueous two-phase systems
Trends Biotechnol. 1991 Nov;9(11):381-8. doi: 10.1016/0167-7799(91)90130-a.
J Huddleston 1 , A Veide, K Köhler, J Flanagan, S O Enfors, A Lyddiatt
1 Biochemical Recovery Group, School of Chemical Engineering, University of Birmingham, UK.
Protein purification based on partition in aqueous two-phase systems has attracted interest for many years. This approach has been advocated as a primary-stage unit operation in downstream processing. In reality, application has been strictly limited through inadequate understanding of the complex molecular forces involved in partitioning processes.