nucleus (Xrcc1) intracellular (Xrcc1)
Xrcc1 (Mus musculus)
Stable suppression of the XRCC1 protein was achieved using the targeting shRNA system, while the scramble-shRNA infected cells retained XRCC1 expression.
Negative_regulation (suppression) of XRCC1
XRCC1 is a key component of SSBR, as cells defective in this nonenzymatic scaffold protein show reduced SSBR, enhanced sensitivity to DNA-damaging agents that produce SSBs, and increased sister chromatid exchange (5).
Negative_regulation (defective) of XRCC1
To determine the functional consequence of XRCC1 down-regulation, we evaluated methyl methanesulfonate (MMS) sensitivity.
Spec (determine) Negative_regulation (regulation) of XRCC1
Using hypoxanthinexanthine oxidase as the oxidizing method, XRCC1 deficiency affected both dividing and nondividing SH-SY5Y cells, with a greater effect on survival seen in the former case, suggesting that the spectrum of oxidative DNA damage created dictates the specific contribution of XRCC1 to cellular resistance.
Negative_regulation (deficiency) of XRCC1 in SH-SY5Y
Shown is a representative western blot of XRCC1 protein expression in dividing XKD and control cells. ?
Negative_regulation (blot) of XRCC1