other: Study is similar to OECD guideline regulatory studies, methods are well reported, but study is not GLP
An adequate in vivo GPMT study is already available
Reading: 1st reading. . Hours after challenge: 24.0. Group: test group. Dose level: 0.1 mL 50% dilution. No with. + reactions: 0.0. Total no. in groups: 20.0. Clinical observations: None.
Reading: 2nd reading. . Hours after challenge: 48.0. Group: test group. Dose level: 0.1 mL 50% dilution. No with. + reactions: 0.0. Total no. in groups: 20.0. Clinical observations: None.
A study using such a formulation is considered relevant to use for this substance since such formulations represent 95% of the end use of this substance.
Brake Fluid DOT 4 Super was tested for sensitisation in Guinea pigs using the Magnusson and Kligman assay. No evidence of sensitsation was observed, and the formulation was considered to be non-sensitising.