Presentation: Antimicrobial Activity of N-Halamine Coated Materials in Broiler Chicken Houses (IAFP 2016 Annual Meeting)
T7-04 Antimicrobial Activity of N-Halamine Coated Materials in Broiler Chicken Houses
Tian Ren, Auburn University, Auburn, AL
Mingyu Qiao, Auburn University, Auburn, AL
Lei Zhang, Auburn University, Auburn, AL
Jean Weese, Auburn University, Auburn, AL
Introduction: Broiler chicken houses are usually at a high risk of microbial cross-contamination. Newly released Food Safety Modernization Act (FSMA) regulation emphasizes the role of production procedure: the initial load of foodborne pathogens may significantly affect the safety of final products.
Purpose: To maintain an optimal hygiene condition, N-halamines are considered as ideal antimicrobial materials due to their superior antimicrobial efficiency against a broad spectrum of bacteria, low toxicity, high stability, rechargeability of antimicrobial activity, and low cost. Our purpose in this study was to investigate the antimicrobial activity of 1-chloro-2,2,5,5-tetramethyl-4-imidazoidinone (MC), a non-bleaching N-halamine compound, and its coated materials in broiler chicken houses.
Methods: The storage stability and the antimicrobial longevity of MC coated materials such as galvanized metal, aluminum, woods, etc. were studied. We used iodometric/thiosulfate titration to assess the chlorine content, and the “sandwich test” method to test the antimicrobial activity over a four-week period.
Results: Results showed that 0.04% MC in aqueous solution were able to kill Salmonella Typhimurium and Campylobacter jejuni completely at 106 CFU/ml within 30 min. Moreover, 1% MC treated samples possessed high antimicrobial activity and were able to kill Salmonella Typhimurium and C. jejuni completely in 2 h. The antimicrobial activity of MC treated materials remained up to 4 week, and the active chlorine atoms in the treated materials decreased from the initial 1016 atoms/cm2 to 1015 atoms/cm2.
Significance: Our results suggest that MC has a great potential application as a novel antimicrobial agent in broiler chicken houses during production.