Interpret the results to the following experiment. Transcriptional activity of chemotactic genes showed a high expression level during the following conditions: repelling away from compound 1, moving towards compound 2, and remaining sessile when presented compounds 3 and 4.
A. Activity during movement in any direction led to high measurements observed, and the sessile population responded equally to both a chemoattractant and a chemorepellant but remained in the same location for their net movement.
B. Gene expression by itself cannot distinguish between cells responding to both an attractant and repellent, so this ambiguity makes transcriptomics unfavorable but nonetheless indicates activity.
C. The activity observed during a sessile existence suggests the molecular probe was targeting a chemotactic gene that is also involved in other non-chemotactic functions due to the activity observed.
D. Transcriptomics as a whole cannot be used for chemotaxis genes because they are not regulated at the transcriptional level (i.e., they are constitutive), which is why activity is observed on all three conditions.
Answer:D
Interpret the results to the following experiment. Transcriptional activity of chemotactic genes showed a high expression level during the following conditions: repelling away from compound 1, moving towards compound 2, and remaining sessile when presented compounds 3 and 4.
A. Activity during movement in any direction led to high measurements observed, and the sessile population responded equally to both a chemoattractant and a chemorepellant but remained in the same location for their net movement. B. Gene expression by itself cannot distinguish between cells responding to both an attractant and repellent, so this ambiguity makes transcriptomics unfavorable but nonetheless indicates activity. C. The activity observed during a sessile existence suggests the molecular probe was targeting a chemotactic gene that is also involved in other non-chemotactic functions due to the activity observed. D. Transcriptomics as a whole cannot be used for chemotaxis genes because they are not regulated at the transcriptional level (i.e., they are constitutive), which is why activity is observed on all three conditions.