During the biotransformation of a large molecular weight compound, two major products are formed. While one of the products is catabolized, the other compounds accumulation represses the biotransformation of the large compound. Which approach is MOST likely to artificially enhance the biotransformation beyond this point in a batch culture?
A. addition of an isoenzyme for the biotransformation route to increase its transcription
B. addition of inducer molecules into the medium to increase transcriptional repression
C. inclusion of more of an intermediate compound in the catabolic pathway
D. spike in additional activator proteins that control the transcription of this pathway
Answer:A
During the biotransformation of a large molecular weight compound, two major products are formed. While one of the products is catabolized, the other compounds accumulation represses the biotransformation of the large compound. Which approach is MOST likely to artificially enhance the biotransformation beyond this point in a batch culture?
A. addition of an isoenzyme for the biotransformation route to increase its transcription B. addition of inducer molecules into the medium to increase transcriptional repression C. inclusion of more of an intermediate compound in the catabolic pathway D. spike in additional activator proteins that control the transcription of this pathway