The order for the construction of a cDNA fragment from mRNA is to
A. bind oligo-dT, treat with reverse transcriptase, digest with RNase, add G residues to the 3′ end, bind oligo-dC, treat with DNA polymerase
B. treat with reverse transcriptase, digest with RNase, add G residues to the 3′ end, bind oligo-dC, treat with DNA polymerase and bind oligo-dT
C. digest with RNase, add G residues to the 3′ end, treat with reverse transcriptase, add G residues to the 3′ end and treat with DNA polymerase
D. bind oligo-dC, treat with reverse transcriptase, digest with RNase, add G residues to the 3′ end, bind oligo-dT and treat with DNA polymerase
Answer:A
The order for the construction of a cDNA fragment from mRNA is to
A. bind oligo-dT, treat with reverse transcriptase, digest with RNase, add G residues to the 3′ end, bind oligo-dC, treat with DNA polymerase B. treat with reverse transcriptase, digest with RNase, add G residues to the 3′ end, bind oligo-dC, treat with DNA polymerase and bind oligo-dT C. digest with RNase, add G residues to the 3′ end, treat with reverse transcriptase, add G residues to the 3′ end and treat with DNA polymerase D. bind oligo-dC, treat with reverse transcriptase, digest with RNase, add G residues to the 3′ end, bind oligo-dT and treat with DNA polymerase