Cloning of eukaryotic DNA into this plasmid that you want to express in E. coli would most likely
A. require the construction of eukaryotic genomic DNA library
B. require that you construct a DNA library in yeast cells first
C. begin with isolation of eukaryotic nuclear DNA
D. begin with isolation of mRNA from eukaryotic cells
Answer:D
Cloning of eukaryotic DNA into this plasmid that you want to express in E. coli would most likely
A. require the construction of eukaryotic genomic DNA library B. require that you construct a DNA library in yeast cells first C. begin with isolation of eukaryotic nuclear DNA D. begin with isolation of mRNA from eukaryotic cells