Which of the following correctly identifies the role of something we used in this experiment?
A. Spectrophotometer, to determine the concentration of DCIP (as % transmittance is proportional to the concentration of DCIP)
B. Azide was used in all the test tubes to provide an oxygen-free environment
C. Mitochondrial suspension was placed in all the test tubes primarily to provide a substrate that the enzymes could bind to
D. DCIP was used to catalyse the reaction; the concentration of it indicated how much was left unchanged, and thus how quickly the reaction proceeded
Answer:B
Which of the following correctly identifies the role of something we used in this experiment?
A. Spectrophotometer, to determine the concentration of DCIP (as % transmittance is proportional to the concentration of DCIP) B. Azide was used in all the test tubes to provide an oxygen-free environment C. Mitochondrial suspension was placed in all the test tubes primarily to provide a substrate that the enzymes could bind to D. DCIP was used to catalyse the reaction; the concentration of it indicated how much was left unchanged, and thus how quickly the reaction proceeded