What happens during the ‘elution from the column’ phase chromatography?
A. Components with the greatest affinity elute first
B. Components with least affinity elute first
C. Components elute in a random manner
D. Components elute according to their concentration in the mixture
Answer:B
What happens during the ‘elution from the column’ phase chromatography?
A. Components with the greatest affinity elute first B. Components with least affinity elute first C. Components elute in a random manner D. Components elute according to their concentration in the mixture
Explanation: During the elution phase, different components elute at different times. Components with least affinity elute first.