Some restriction enzymes do not leave sticky ends when cutting DNA molecules; rather, they cut a restriction site down the middle and leave “blunt-ended” DNA molecules, which do not have any single-stranded nucleotide extensions. Which of the following is not a likely outcome when trying to insert a gene into a plasmid when have both been cut with the same “blunt-ended” restriction enzyme?
A. The gene might be inserted into the plasmid by forming hydrogen bonds.
B. The gene might be inserted into the plasmid multiple times in a row.
C. The gene might insert into the plasmid in the proper (forward) orientation.
D. The gene might insert into the plasmid in the wrong (backwarD. orientation.
Answer:A
Some restriction enzymes do not leave sticky ends when cutting DNA molecules; rather, they cut a restriction site down the middle and leave “blunt-ended” DNA molecules, which do not have any single-stranded nucleotide extensions. Which of the following is not a likely outcome when trying to insert a gene into a plasmid when have both been cut with the same “blunt-ended” restriction enzyme?
A. The gene might be inserted into the plasmid by forming hydrogen bonds. B. The gene might be inserted into the plasmid multiple times in a row. C. The gene might insert into the plasmid in the proper (forward) orientation. D. The gene might insert into the plasmid in the wrong (backwarD. orientation.