Hfr (thr+ leu+ gal+ strs) × F- (thr- leu- gal- strr). Which of the following selective media would you use to score recombinant colonies?
A. minimal medium
B. minimal medium + streptomycin
C. minimal medium + threonine
D. minimal medium + streptomycin + threonine
Answer:B
Hfr (thr+ leu+ gal+ strs) × F- (thr- leu- gal- strr). Which of the following selective media would you use to score recombinant colonies?
A. minimal medium B. minimal medium + streptomycin C. minimal medium + threonine D. minimal medium + streptomycin + threonine