In order to stop the reaction between enzyme and substrate, what process is needed?
A.) Incubate the sample at 100oC
B.) Add strong acid to sample
C.) Wash sample with PBS-Tween
D.) Add blocking solution to sample
Answer:B
In order to stop the reaction between enzyme and substrate, what process is needed?
A.) Incubate the sample at 100oC B.) Add strong acid to sample C.) Wash sample with PBS-Tween D.) Add blocking solution to sample
Explanation: In the experiment, strong acid H2SO4 is added to sample to stop the enzyme reaction.