Immunoelectrophoresis techniques are used to separate the components of a mixture using electrophoresis.
A.) prior to reaction with antibody
B.) prior to reaction with antigen
C.) after reaction with antibody
D.) after reaction with antigen
Answer:A
Immunoelectrophoresis techniques are used to separate the components of a mixture using electrophoresis.
A.) prior to reaction with antibody B.) prior to reaction with antigen C.) after reaction with antibody D.) after reaction with antigen
Explanation: Immunoelectrophoresis is a technique used to separate the components of a mixture using electrophoresis. It combines the principles of zone electrophoresis and immunodiffusion, and requires immunoglobulins, also known as antibodies, to react with the proteins to be separated or characterized [1]. Immunoelectrophoresis is used to detect normal and abnormal proteins in human serum, such as myeloma proteins [2]. It is also used to analyze complex protein mixtures containing different antigens [3]. The method combines the principles of zone electrophoresis and immunodiffusion, and is still used by some clinical laboratories [4]. It is also used to identify and characterize proteins within complex mixtures [5].