<p><b>1-乙基-3-(3-二甲基氨基丙基)碳二亞胺</b>（亦稱為<b>EDC</b>、<b>EDAC</b>或<b>EDCI</b>）是一種水溶性碳二亞胺，常製成鹽酸鹽之形式。其pH範圍通常在4.0-6.0。EDC常在伯胺製備醯氨時作為羧基活化劑。EDC還可用於活化磷酸基團。這種碳二亞胺通常用於肽的合成；蛋白質和核酸的交聯以及製備免疫偶聯物。EDC常與N-羥基丁二醯亞胺（NHS）或磺化－NHS搭配使用從而增加偶聯反應的效率。
</p><p>有機化學中，EDC還常與4-二甲氨基吡啶（DMAP）搭配使用作為羧酸和醇的酯化試劑，稱為施特格利希酯化反應。
</p>


<h2><span id=".E5.88.B6.E5.A4.87"></span><span id="制备">製備</span></h2>
<p>EDC為商業可售試劑，可通過乙基異氰酸酯和<i>N</i>,<i>N</i>-二甲基丙烷-1,3-二胺反應得脲，而後繼續脫水製得：</p>
<dl><dd></dd></dl><h2><span id=".E5.A4.96.E9.83.A8.E9.93.BE.E6.8E.A5"></span><span id="外部链接">外部連結</span></h2>
<ul><li>EDCI coupling - Synthetic protocols from organic-reaction.com</li></ul><h2><span id=".E5.8F.82.E8.80.83.E6.96.87.E7.8C.AE"></span><span id="参考文献">參考文獻</span></h2>
<h2><span id=".E5.BB.B6.E4.BC.B8.E9.98.85.E8.AF.BB"></span><span id="延伸阅读">延伸閱讀</span></h2>
<ul><li><cite class="citation journal">López-Alonso, JP; Diez-Garcia, F; Font, J; Ribó, M; Vilanova, M; Scholtz, JM; González, C; Vottariello, F; Gotte, G; Libonati, M; Laurents, DV. Carbodiimide EDC Induces Cross-Links That Stabilize RNase A C-dimer against Dissociation: EDC Adducts Can Affect Protein Net Charge, Conformation and Activity. Bioconjug Chem. 2009, <b>20</b>: 1459. <span title="數位物件識別號">doi:10.1021/bc9001486</span>.</cite><span title="ctx_ver=Z39.88-2004&amp;rfr_id=info%3Asid%2Fzh.wikipedia.org%3A1-%E4%B9%99%E5%9F%BA-%283-%E4%BA%8C%E7%94%B2%E5%9F%BA%E6%B0%A8%E5%9F%BA%E4%B8%99%E5%9F%BA%29%E7%A2%B3%E4%BA%8C%E4%BA%9A%E8%83%BA&amp;rft.atitle=Carbodiimide+EDC+Induces+Cross-Links+That+Stabilize+RNase+A+C-dimer+against+Dissociation%3A+EDC+Adducts+Can+Affect+Protein+Net+Charge%2C+Conformation+and+Activity&amp;rft.au=L%C3%B3pez-Alonso%2C+JP%3B+Diez-Garcia%2C+F%3B+Font%2C+J%3B+Rib%C3%B3%2C+M%3B+Vilanova%2C+M%3B+Scholtz%2C+JM%3B+Gonz%C3%A1lez%2C+C%3B+Vottariello%2C+F%3B+Gotte%2C+G%3B+Libonati%2C+M%3B+Laurents%2C+DV&amp;rft.date=2009&amp;rft.genre=article&amp;rft.jtitle=Bioconjug+Chem.&amp;rft.pages=1459&amp;rft.volume=20&amp;rft_id=info%3Adoi%2F10.1021%2Fbc9001486&amp;rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal"><span> </span></span></li>
<li><cite class="citation journal">Nakajima, N; Ikada, Y. Mechanism of Amide Formation by Carbodiimide for Bioconjugation in Aqueous Media. Bioconjug Chem. 1995, <b>6</b>: 123. <span title="數位物件識別號">doi:10.1021/bc00031a015</span>.</cite><span title="ctx_ver=Z39.88-2004&amp;rfr_id=info%3Asid%2Fzh.wikipedia.org%3A1-%E4%B9%99%E5%9F%BA-%283-%E4%BA%8C%E7%94%B2%E5%9F%BA%E6%B0%A8%E5%9F%BA%E4%B8%99%E5%9F%BA%29%E7%A2%B3%E4%BA%8C%E4%BA%9A%E8%83%BA&amp;rft.atitle=Mechanism+of+Amide+Formation+by+Carbodiimide+for+Bioconjugation+in+Aqueous+Media&amp;rft.au=Nakajima%2C+N%3B+Ikada%2C+Y&amp;rft.date=1995&amp;rft.genre=article&amp;rft.jtitle=Bioconjug+Chem.&amp;rft.pages=123&amp;rft.volume=6&amp;rft_id=info%3Adoi%2F10.1021%2Fbc00031a015&amp;rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal"><span> </span></span></li>
<li><cite class="citation journal">Skotnicki, S; Kearney, RM; Smith, AL. Synthesis of secorapamycin esters and amides. Tetrahedron Lett. 1994, <b>35</b>: 197. <span title="數位物件識別號">doi:10.1016/S0040-4039(00)76509-9</span>.</cite><span title="ctx_ver=Z39.88-2004&amp;rfr_id=info%3Asid%2Fzh.wikipedia.org%3A1-%E4%B9%99%E5%9F%BA-%283-%E4%BA%8C%E7%94%B2%E5%9F%BA%E6%B0%A8%E5%9F%BA%E4%B8%99%E5%9F%BA%29%E7%A2%B3%E4%BA%8C%E4%BA%9A%E8%83%BA&amp;rft.atitle=Synthesis+of+secorapamycin+esters+and+amides&amp;rft.au=Skotnicki%2C+S%3B+Kearney%2C+RM%3B+Smith%2C+AL&amp;rft.date=1994&amp;rft.genre=article&amp;rft.jtitle=Tetrahedron+Lett.&amp;rft.pages=197&amp;rft.volume=35&amp;rft_id=info%3Adoi%2F10.1016%2FS0040-4039%2800%2976509-9&amp;rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal"><span> </span></span></li></ul>
**1 - 乙基 - 3-(3 - 二甲基氨基丙基) 碳二亞胺**（亦稱為 **EDC**、**EDAC** 或 **EDCI**）是一種水溶性碳二亞胺，常製成鹽酸鹽之形式。其 pH 範圍通常在 4.0-6.0。EDC 常在伯胺製備醯氨時作為羧基活化劑。EDC 還可用於活化磷酸基團。這種碳二亞胺通常用於肽的合成；蛋白質和核酸的交聯以及製備免疫偶聯物。EDC 常與 N - 羥基丁二醯亞胺（NHS）或磺化－NHS 搭配使用從而增加偶聯反應的效率。

有機化學中，EDC 還常與 4 - 二甲氨基吡啶（DMAP）搭配使用作為羧酸和醇的酯化試劑，稱為施特格利希酯化反應。

## 製備

EDC 為商業可售試劑，可通過乙基異氰酸酯和 _N_,_N_- 二甲基丙烷 - 1,3 - 二胺反應得脲，而後繼續脫水製得：

　
:

## 外部連結

* EDCI coupling - Synthetic protocols from organic-reaction.com

## 參考文獻

## 延伸閱讀

* López-Alonso, JP; Diez-Garcia, F; Font, J; Ribó, M; Vilanova, M; Scholtz, JM; González, C; Vottariello, F; Gotte, G; Libonati, M; Laurents, DV. Carbodiimide EDC Induces Cross-Links That Stabilize RNase A C-dimer against Dissociation: EDC Adducts Can Affect Protein Net Charge, Conformation and Activity. Bioconjug Chem. 2009, **20**: 1459. doi:10.1021/bc9001486.
* Nakajima, N; Ikada, Y. Mechanism of Amide Formation by Carbodiimide for Bioconjugation in Aqueous Media. Bioconjug Chem. 1995, **6**: 123. doi:10.1021/bc00031a015.
* Skotnicki, S; Kearney, RM; Smith, AL. Synthesis of secorapamycin esters and amides. Tetrahedron Lett. 1994, **35**: 197. doi:10.1016/S0040-4039(00)76509-9.
1-乙基-(3-二甲基氨基丙基)碳二亚胺