Aunt May
She was played by Rosemary Harris in Spider-Man (2002), Spider-Man 2 (2004), and in Spider-Man 3 (2007). She was then played by Sally Field in The Amazing Spider-Man (2012), and The Amazing Spider-Man 2 (2014). She is played by Marisa Tomei in the Marvel Cinematic Universe movies Captain America: Civil War (2016), Spider-Man: Homecoming (2017), Spider-Man: Far From Home (2019), and in Spider-Man: No Way Home (2021).