Size-exclusion chromatography
SEC has several advantages and disadvantages. First in SEC the time it takes the mixture to exit the column can be controlled. Depending on the chemical characteristics of the mixture and its components the process could be quick or slow. Second the peaks are well-defined, sharp, and narrow. All characteristics indicate SCE provides good sensitivity. Next there is low mass loss since the sample theoretically is not supposed to come interact with the stationary phase. Last the stability of the stationary phase allows close packing to provide greater separation of components in the sample.