Deacetylase involved in the biosynthesis of neomycin by mediating 2 steps of the pathway. Deacetylates both 2'-N-acetylparomamine and 2'''-acetyl-6'''-hydroxyneomycin C. 2'-N-acetylparomamine + H2O = acetate + paromamine 2'''-acetyl-6'''-hydroxyneomycin C + H2O = 6'''-deamino-6'''-hydroxyneomycin C + acetate Binds 1 zinc ion per subunit. Antibiotic biosynthesis; neomycin biosynthesis. Belongs to the PIGL family. Truncated N-terminus.