Facilitates transcription termination by a mechanism that involves Rho binding to the nascent RNA, activation of Rho's RNA-dependent ATPase activity, and release of the mRNA from the DNA template. Shows poor RNA-dependent ATP hydrolysis and inefficient DNA-RNA unwinding activities, but exhibits robust and fast transcription termination, which suggests that the transcription termination function of M.tuberculosis Rho is not correlated with its helicase/translocase activities and that these functions may not be important for its RNA release process. The antibiotic bicyclomycin inhibits ATPase activity but does not affect termination function. Homohexamer. The homohexamer assembles into an open ring structure. Does not bind NusG. Contains an extra N-terminal domain, a S1-like RNA-binding domain and a conserved P-loop NTPase domain. Was identified as a high-confidence drug target. Belongs to the Rho family.