Non-hemorrhagic proteinase that activates prothrombin (F2) calcium-independently. Activates factor X (F10) and hydrolyzes the Aalpha-chain and more slowly the Bbeta-chain of fibrin and fibrinogen without affecting the gamma chain. It induces neither detachment nor apoptosis of human endothelial cells and is also not able to trigger an endothelial pro-inflammatory cell response. Nitric oxide and prostacyclin levels released by endothelial cells are significantly increased after treatment with insularinase A. Inhibits ADP-induced platelet aggregation (IC(50)=0.8 uM for native protein) (PubMed:21073888). Interestingly, inhibits the adhesion of HUVECs to immobilized fibrinogen at very low concentrations (IC(50)=36 nM) (PubMed:21073888). Binds 1 zinc ion per subunit. Inhibited by EDTA, and 1,10-phenanthroline, but not by PMSF. Monomer (metalloprotease). Expressed by the venom gland. The N-terminus is blocked. Not glycosylated. Belongs to the venom metalloproteinase (M12B) family. P-II subfamily. P-IIa sub-subfamily.