a phosphate monoester + H2O = an alcohol + phosphate Activated slightly by Co(2+), Ca(2+) and Mg(2+). Strongly inhibited by NaF, Fe(3+) and Al(3+), less so by tartrate, Cu(2+) and Hg(+). Not affected by EDTA, Fe(2+) and Zn(2+). Optimum pH is 4.6. Activity decreases sharply with decreases and increases in pH and is absent at pH 8.0. When pre-incubated at different pH levels, activity is stable between pH 4 and 9. Optimum temperature is 50 degrees Celsius. Activity decreases sharply with decreases and increases in temperature. When pre-incubated at different temperatures, activity is stable between 20 and 50 degrees Celsius. Monomer. Has no activity towards sodium phytate, ATP, ADP, AMP, glucose-6-phosphate, fructose-6-phosphate and beta-glycerophosphate.