Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the sulfate conjugation of desulfo-glucosinolates (dsGSs), the final step in the biosynthesis of the glucosinolate core structure. Substrate preference is desulfo-2-phenylethyl glucosinolate > desulfo-indol-3-yl methyl glucosinolate > desulfo-benzyl glucosinolate > desulfo-6-methylthiohexyl glucosinolate > desulfo-4-methylthiobutyl glucosinolate > desulfo-3-methylthiopropyl glucosinolate > desulfo-singrin > desulfo-3-butenyl glucosinolate. (Z)-desulfoglucotropeolin + 3'-phosphoadenylyl sulfate = (Z)-glucotropeolin + adenosine 3',5'-bisphosphate + H(+) (Z)-indolylmethyl desulfoglucosinolate + 3'-phosphoadenylyl sulfate = (Z)-glucobrassicin + adenosine 3',5'-bisphosphate + H(+) Inhibited by phosphoadenosine 5'-phosphate (PAP). Optimum pH is 9.0. Highly expressed in roots, stems and mature leaves. Low expression in young leaves and flowers. Barely detected in siliques. Expression reaching a maximum in 2-week-old plants and a minimum in flowering plants. By wounding, 12-oxophytodienoic acid, jasmonic acid, ethylene, UV-C and coronatine treatments. Not induced by abscisic acid, 2,4-dichlorophenoxyacetic acid, gibberellin, kinetin, salicylic acid, yeast elicitor or high sulfate concentration. Belongs to the sulfotransferase 1 family.