Shows esterase and phospholipase A activities (PubMed:19169353, PubMed:19225166, PubMed:20656688, PubMed:29247008). May be involved in cell wall biosynthesis and/or maintenance (PubMed:19169353, PubMed:19225166, PubMed:20656688). Can hydrolyze various substrates, including the p-nitrophenol-linked aliphatic esters pNP-laurate (C12), pNP-myristate (C14), pNP-palmitate (C16), pNP-stearate (C18), pNP-butyrate (C4), phosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, 4-methylumbelliferyl heptanoate and palmitic acid and arachidonic acid containing phospholipids (PubMed:19169353, PubMed:19225166, PubMed:20656688). Does not exhibit cutinase activity (PubMed:19225166). a dodecanoate ester + H2O = an aliphatic alcohol + dodecanoate + H(+) a tetradecanoate ester + H2O = an aliphatic alcohol + H(+) + tetradecanoate H2O + hexadecanoate ester = an aliphatic alcohol + H(+) + hexadecanoate H2O + octadecanoate ester = an aliphatic alcohol + H(+) + octadecanoate a butanoate ester + H2O = an aliphatic alcohol + butanoate + H(+) 1,2-di-(9Z)-octadecenoyl-sn-glycero-3-phospho-L-serine + H2O = (9Z)-octadecenoate + 1-(9Z-octadecenoyl)-sn-glycero-3-phospho-L-serine + H(+) 1,2-dihexadecanoyl-sn-glycero-3-phosphocholine + H2O = 1-hexadecanoyl-sn-glycero-3-phosphocholine + H(+) + hexadecanoate 1-acyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phosphocholine + H2O = (5Z,8Z,11Z,14Z)-eicosatetraenoate + a 1-acyl-sn-glycero-3-phosphocholine + H(+) 1-hexadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phosphoethanolamine + H2O = (5Z,8Z,11Z,14Z)-eicosatetraenoate + 1-hexadecanoyl-sn-glycero-3-phosphoethanolamine + H(+) H2O + hexadecanoyl-CoA = CoA + H(+) + hexadecanoate decanoyl-CoA + H2O = CoA + decanoate + H(+) Inhibited by tetrahydrolipstatin (THL), a specific lipase inhibitor, and by derivatives of THL (PubMed:19169353, PubMed:20656688, PubMed:21384024). Inhibited by high concentrations of paraoxon (PubMed:19225166). Also inhibited by a Furan-based urea derivative, 1-(3,5-difluorophenyl)-3-(furan-2-ylmethyl)urea (PubMed:31741730). kcat is 0.00881 sec(-1) with nitrophenyl butyrate as substrate. kcat is 0.0733 sec(-1) with palmitoyl-S-CoA as substrate. kcat is 0.0845 sec(-1) with decanoyl-S-CoA as substrate (PubMed:19169353). kcat is 0.143 sec(-1) with pNP-butyrate as substrate (PubMed:20656688). kcat is 10.05 min(-1) with 4-methylumbelliferyl heptanoate as substrate (PubMed:29247008). Optimum pH is above 7.0 for lipase activity. The essential nature of the cell wall protein Culp6 makes it a promising target for drug development. Belongs to the cutinase family.