creatine + H2O = sarcosine + urea Homodimer. Each monomer has two clearly defined domains. The small N-terminal domain (AA 1-161) and the large domain (AA 162-403). Each of the two active sites is made by residues of the large domain of one monomer and some residues of the small domain of the other monomer. Belongs to the peptidase M24 family. Creatinase subfamily.