In vitro, has activity towards 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS), 2,6-dimethoxy-phenol, and guaiacol (PubMed:34116754). Although brown rot fungi preferentially degrade hemicellulose and cellulose, the enzyme may contribute to generating small amounts of lignin breakdown products required for catalytic reactions (PubMed:34116754). 4 hydroquinone + O2 = 4 benzosemiquinone + 2 H2O Binds 4 Cu cations per monomer. Inhibited by chloride ions (PubMed:34116754). Inhibited by citrate (PubMed:34116754). Inhibited by oxalate (PubMed:34116754). Activated by acetate (PubMed:34116754). kcat is 409 sec(-1) with 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) as substrate (at pH 3.0 and 30 degrees Celsius). kcat is 23.1 sec(-1) with 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) as substrate (at pH 5.0 and 30 degrees Celsius). kcat is 102 sec(-1) with 2,6-dimethoxy-phenol as substrate (at pH 3.0 and 30 degrees Celsius). kcat is 7.89 sec(-1) with 2,6-dimethoxy-phenol as substrate (at pH 5.0 and 30 degrees Celsius). cat is 85.1 sec(-1) with guaiacol as substrate (at pH 3.0 and 30 degrees Celsius). kcat is 13.2 sec(-1) with guaiacol as substrate (at pH 5.0 and 30 degrees Celsius). Optimum pH is 2.5 or below with 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) or 2,6-dimethoxy-phenol as substrate, and 3.5 with guaiacol as substrate. Expressed during growth on poplar wood (at protein level). Belongs to the multicopper oxidase family.