Plays a role in the different steps of crossover formation during meiotic recombination (PubMed:24891606, PubMed:32640224, PubMed:32555348). Participates in the crossover differentiation step of crossover-specific recombination intermediates through its interaction with PRR19 (PubMed:32555348). In addition, stimulates crossover formation through the interactions with RFC3 and RFC4 and simultaneously regulates cell-cycle progression through interactions with CDC34 and subsequent ubiquitination of WEE1 (PubMed:32640224). May also participates in an active deselection process that destabilizes or removes excess pre-CO intermediates (PubMed:24891606). Interacts with PRR19; this interaction promotes crossover formation (PubMed:32555348). Interacts with RFC3 and RFC4; these interactions facilitate crossover formation (PubMed:32640224). Interacts with CDC34; this interaction regulates the cell-cycle progression (PubMed:32640224). Shuttles between the nucleus and cytoplasm in a stage-specific manner of prophase I cells (PubMed:32640224). Co-localized at crossover sites with PRR19 (PubMed:32555348). Isoform 2 is expressed in spermatocyte. Homozygous knockout mice for CNTD1 are grossly similar to wild-type, surviving into adulthood and exhibiting appropriate mating behavior. Males are sterile with a decreased testis size. Females are also sterile. Probable cloning artifact.