Ceramide synthase that catalyzes the transfer of the acyl chain from acyl-CoA to a sphingoid base, with high selectivity toward palmitoyl-CoA (hexadecanoyl-CoA; C16:0-CoA)(PubMed:16951403, PubMed:18541923, PubMed:22144673, PubMed:22661289, PubMed:23530041, PubMed:26887952, PubMed:29632068, PubMed:31916624). Can use other acyl donors, but with less efficiency (By similarity). N-acylates sphinganine and sphingosine bases to form dihydroceramides and ceramides in de novo synthesis and salvage pathways, respectively (PubMed:31916624). Plays a role in de novo ceramide synthesis and surfactant homeostasis in pulmonary epithelia (By similarity). a sphingoid base + hexadecanoyl-CoA = an N-hexadecanoyl-sphingoid base + CoA + H(+) hexadecanoyl-CoA + sphinganine = CoA + H(+) + N-hexadecanoylsphinganine hexadecanoyl-CoA + hexadecasphinganine = CoA + H(+) + N-hexadecanoylhexadecasphinganine hexadecanoyl-CoA + sphing-4-enine = CoA + H(+) + N-hexadecanoylsphing-4-enine 2-hydroxyhexadecanoyl-CoA + sphinganine = CoA + H(+) + N-(2-hydroxyhexadecanoyl)-sphinganine sphinganine + tetradecanoyl-CoA = CoA + H(+) + N-(tetradecanoyl)-sphinganine octadecanoyl-CoA + sphinganine = CoA + H(+) + N-(octadecanoyl)-sphinganine (9Z)-octadecenoyl-CoA + sphinganine = CoA + H(+) + N-(9Z-octadecenoyl)-sphinganine a fatty acyl-CoA + sphing-4-enine = an N-acylsphing-4-enine + CoA + H(+) Inhibited by fumonisin B1. Lipid metabolism; sphingolipid metabolism. The last loop motif confers selectivity toward palmitoyl-CoA (hexadecanoyl-CoA; C16:0-CoA) as acyl donor. Phosphorylated at the C-terminus by CK2. Some prediction bioinformatics tools predict the presence of a homeobox domain (By similarity). However, the domain is degenerate and residues that are important for DNA-binding are absent (By similarity).