Has high processivity, a high polymerization rate and high fidelity. In addition to polymerase activity, also exhibits 3' to 5' exonuclease activity. Intein encoded endonucleases are thought to mediate intein mobility by site-specific recombination initiated by endonuclease cleavage at the 'homing site' in genes that lack the intein (Probable). Upon expression in E.coli PI-PkoI recognizes the minimal sequence 5'-GATTTTAGATCCCTGTACC-3' and cuts after T-10. PI-PkoII recognizes the minimal sequence 5'-CAGCTACTACGGTTAC-3' and cuts after C-10. Given the high intracellular K(+) content (>0.5 M), PI-PkoII is probably more active than PI-PkoI in vivo (PubMed:9742242). a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) = diphosphate + DNA(n+1) DNA polymerase optimum pH is 6.5, very thermostable, has a half-life of 12 hours at 95 and 3 hours at 100 degrees Celsius, for protein cloned without inteins in E.coli. DNA polymerase optimum temperature is 75 degrees Celsius, for protein cloned without inteins in E.coli (PubMed:9361436). Both intein endonucleases are thermostable (PubMed:9742242). Undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation. Pyrococcus kodakaraensis enzyme is a very fast, thermostable DNA polymerase (130 nucleotides/s) used in high-performance PCR. Belongs to the DNA polymerase type-B family.