As part of the OOEP-KHDC3 scaffold, recruits BLM and TRIM25 to DNA replication forks, thereby promoting the ubiquitination of BLM by TRIM25, enhancing BLM retainment at replication forks and therefore promoting stalled replication fork restart (PubMed:29125140, PubMed:33115731). Regulates homologous recombination-mediated DNA repair via recruitment of RAD51 to sites of DNA double-strand breaks, and sustainment of PARP1 activity, which in turn modulates downstream ATM or ATR activation (PubMed:25936915, PubMed:33115731). Activation of ATM or ATR in response to DNA double-strand breaks may be cell-type specific (PubMed:25936915, PubMed:33115731). Its role in DNA double-strand break repair is independent of its role in restarting stalled replication forks (PubMed:29125140). As a member of the subcortical maternal complex (SCMC), plays an essential role for zygotes to progress beyond the first embryonic cell divisions via regulation of actin dynamics (PubMed:18804437, PubMed:29125140, PubMed:28992324, PubMed:31575650). Required for maintenance of euploidy during cleavage-stage embryogenesis (PubMed:19376971). Required for the formation of F-actin cytoplasmic lattices in oocytes which in turn are responsible for symmetric division of zygotes via the regulation of mitotic spindle formation and positioning (PubMed:25208553, PubMed:31575650). Ensures proper spindle assembly by regulating the localization of AURKA via RHOA signaling and of PLK1 via a RHOA-independent process (PubMed:19376971). Required for the localization of MAD2L1 to kinetochores to enable spindle assembly checkpoint function (PubMed:19376971). Promotes neural stem cell neurogenesis and neuronal differentiation in the hippocampus (PubMed:33115731). May regulate normal development of learning, memory and anxiety (PubMed:33115731). Capable of binding RNA (PubMed:22276159). Component of the subcortical maternal complex (SCMC), at least composed of NLRP5, KHDC3, OOEP, and TLE6 (PubMed:18057100, PubMed:18804437, PubMed:28992324). Within the complex, interacts with NLRP5, OOEP and TLE6 (PubMed:18057100, PubMed:18804437). The SCMC may facilitate translocation of its components between the nuclear and cytoplasmic compartments (By similarity). Forms a scaffold complex with OOEP/FLOPED, and interacts with BLM and TRIM25 at DNA replication forks (PubMed:29125140). Interacts with PARP1; the interaction is increased following the formation of DNA double-strand breaks (PubMed:25936915). Interacts (via C-terminus) with NUMA1 (PubMed:25936915). Localized to centrosomes during interphase and mitosis (PubMed:25936915). Localizes to sites of DNA double-strand break repair (By similarity). Localization to the mitochondrion during apoptosis following phosphorylation of Ser-349 (PubMed:25936915). Expressed in the subcortex of oocytes (PubMed:25208553). Located throughout the cell cortex of ovulated eggs in a complex with NLRP5 (PubMed:18057100). After fertilization, restricted to the apical cortex and excluded from regions of cell-cell contact (PubMed:18057100). Detected in ovary, but not in testis or somatic tissues. In the ovary, expressed in growing oocytes. Expressed in the brain at low levels during fetal development, from 13.5 dpc to 18.5 dpc, however significantly increased after birth from P1 to P60 (PubMed:33115731). Highly expressed in neural stem progenitor cells in the hippocampus after birth (PubMed:33115731). Expressed in growing oocytes but diminishes in fully grown oocytes (PubMed:18057100). Detected at very low levels in morula and early blastocyst (PubMed:18057100). Expressed in growing oocytes, ovulated eggs and preimplantation embryos up to the morula stage and decreases markedly at the blastocyst stage (at protein level). Induced by ultraviolet light, etoposide, doxorubicin, camptothecin and hydroxyl urea in embryonic stem cells (PubMed:25936915). Induced by etoposide and hydroxy urea in neural stem cells (PubMed:33115731). Contains 1 atypical KH domain, which is still capable of binding RNA. Phosphorylation at Ser-151 is required to promote stalled fork restart. Reduced fecundity and impaired preimplantation embryo development with a high incidence of aneuploidy due to abnormal spindle assembly, chromosomal misalignment and spindle assembly checkpoint inactivation (PubMed:19376971). Loss of cytoplasmic lattices and aberrant organelle distribution in oocytes (PubMed:31575650). Increase in abnormal embryonic structure and loss of the embryo following compromised post-implantation embryo development (PubMed:29125140). Knockout mice show normal brain structure and weight (PubMed:33115731). Increased DNA double-strand breaks in hippocampus neural stem cells at P1 to 1 year of age (PubMed:33115731). Reduced hippocampal neural stem cell proliferation from P7 to P60 (PubMed:33115731). Reduced differentiation of neurons and dendritic spines formation in the dentate gyrus from P13 to P66 (PubMed:33115731). Impaired hippocampus-dependent spatial learning, memory and anxiety from 2 months to 1 year of age (PubMed:33115731). Belongs to the KHDC1 family.