Mitochondrial acyltransferase which transfers the acyl group to the N-terminus of glycine (PubMed:22475485, PubMed:20305126). Conjugates numerous substrates, such as arachidonoyl-CoA and saturated medium and long-chain acyl-CoAs ranging from chain-length C8:0-CoA to C18:0-CoA, to form a variety of N-acylglycines. Shows a preference for monounsaturated fatty acid oleoyl-CoA (C18:1-CoA) as an acyl donor. Does not exhibit any activity toward C22:6-CoA and chenodeoxycholoyl-CoA, nor toward serine or alanine (PubMed:20305126). an acyl-CoA + glycine = an N-acylglycine + CoA + H(+) (9Z)-hexadecenoyl-CoA + glycine = CoA + H(+) + N-(9Z-hexadecenoyl)-glycine glycine + octadecanoyl-CoA = CoA + H(+) + N-octadecanoylglycine (5Z,8Z,11Z,14Z)-eicosatetraenoyl-CoA + glycine = CoA + H(+) + N-(5Z,8Z,11Z,14Z)-eicosatetraenoyl-glycine (9Z)-octadecenoyl-CoA + glycine = CoA + H(+) + N-(9Z-octadecenoyl)glycine glycine + octanoyl-CoA = CoA + H(+) + N-octanoylglycine decanoyl-CoA + glycine = CoA + H(+) + N-decanoylglycine glycine + tetradecanoyl-CoA = CoA + H(+) + N-tetradecanoylglycine dodecanoyl-CoA + glycine = CoA + H(+) + N-dodecanoylglycine (9Z,12Z)-octadecadienoyl-CoA + glycine = CoA + H(+) + N-(9Z,12Z-octadecadienoyl)-glycine a fatty acyl-CoA + glycine = an N-(fatty acyl)-glycine + CoA + H(+) Expressed at highest levels in salivary gland and trachea. Also detected in thyroid gland, spinal cord, prostate, lung and fetal brain. Acetylation at Lys-19 drastically decreases the production of N-oleoyl and N-arachidonoyl glycines. Belongs to the glycine N-acyltransferase family.