This is a novel enzyme, catalytically different from common alcohol dehydrogenases. It is effective in oxidizing ethanol, other primary alcohols and benzylalcohol only in the presence of p-nitroso-N,N-dimethylaniline (NDMA) as an electron acceptor. NADH acts as a cofactor here instead of as a coenzyme. a primary alcohol + N,N-dimethyl-4-nitrosoaniline = 4-(hydroxylamino)-N,N-dimethylaniline + an aldehyde A + ethanol = acetaldehyde + AH2 The lower limit of the determination of KM with NDMA as electron acceptor was 0.001 mM. KM was even lower for hexan-1-ol, pentan-1-ol, and citronellol. Optimum pH is 7.0. Homotetramer. Belongs to the zinc-containing alcohol dehydrogenase family.