Glycolytic enzyme that acts in the place of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and phosphoglycerate kinase (PGK). Plays a role in anabolic gluconeogenesis. D-glyceraldehyde 3-phosphate + H2O + 2 oxidized [2Fe-2S]-[ferredoxin] = (2R)-3-phosphoglycerate + 3 H(+) + 2 reduced [2Fe-2S]-[ferredoxin] Binds 1 [4Fe-4S] cluster per subunit. Binds 1 W-bis(molybdopterin guanine dinucleotide) (W-bis-MGD) cofactor per subunit. Activity increased by 58%-93% in the presence of acetyl phosphate, 3-phosphoglycerate or 2,3-bisphosphoglycerate at 10 mM concentration. Inhibited by up to 25% in the presence of crotonaldehyde or formaldehyde at 10 mM concentration. Inhibited by up to 50% by sodium dithionate. 3.5-fold increase in activity observed by addition of potassium phosphate or sodium arsenate at 200 mM concentration. Activity enhanced by potassium chloride, sodium citrate or sodium sulfate at 200 mM concentration. Sensitive to oxygen. Stable at 80 degrees Celsius for 15 minutes. Monomer. Two zinc atoms per subunit with no known function have been observed. Belongs to the AOR/FOR family.