Involved in the control of cytoskeleton formation by regulating actin polymerization. Inhibits actin fiber formation and cell migration (PubMed:25961457). Inhibits RhoA activity; the function involves phosphorylation through PI3K/Akt signaling and may depend on the competitive interaction with 14-3-3 adapter proteins to sequester them from active complexes (PubMed:25961457). Inhibits the formation of lamellipodia but not of filopodia; the function may depend on the competitive interaction with BAIAP2 to block its association with activated RAC1 (PubMed:25961457). Inhibits fibronectin-mediated cell spreading; the function is partially mediated by BAIAP2. Inhibits neurite outgrowth. Involved in the establishment and persistence of cell polarity during directed cell movement in wound healing. In the nucleus, is involved in beta-catenin-dependent activation of transcription. Potential tumor suppressor for renal cell carcinoma. Regulates Rac signaling pathways (PubMed:25961457). Interacts with YWHAQ; the interaction requires KANK1 phosphorylation at Ser-325 and is enhanced by growth factor stimulation (PubMed:18458160). Interacts with YWHAB, YWHAG, YWHAE, YWHAH, YWHAZ and SFN; the interaction requires KANK1 phosphorylation at Ser-325 (PubMed:18458160). Interacts with ARFGEF1; however, colocalization cannot be experimentally confirmed (PubMed:22084092). Interacts with BAIAP2 (PubMed:19171758). Interacts (via ANK repeats 1-5) with KIF21A (via residues 1146-1167); KIF21A enhances translocation of KANK1 to the plasma membrane (PubMed:19559006, PubMed:29183992). Interacts with CTNNB1 (PubMed:16968744). Colocalizes with KIF21A in membrane ruffles (PubMed:19559006). Shuttles between the cytoplasm and nucleus (PubMed:16968744). Shuttles between the cytoplasm and nucleus. Shuttles between the cytoplasm and nucleus. Widely expressed. Isoform 1 is predominantly expressed in heart and kidney. Isoform 2 probably is widely expressed at basic levels. The disease is caused by variants affecting the gene represented in this entry. Extended N-terminus.