Alpha-conotoxins act on postsynaptic membranes, they bind to the nicotinic acetylcholine receptors (nAChR) and thus inhibit them (By similarity). At high doses (10 uM), the synthetic non-amidated toxin (residues 49-65) completely inhibits frog sciatic nerve-gastrocnemius muscle contractility (PubMed:33610632). It also inhibit the cholinergic miniature excitatory postsynaptic currents (mEPSCs) frequency and amplitude of projection neurons in insects (D.melanogaster) (PubMed:33610632). In vivo, the toxin (100 ug/kg) shows good analgesic effects in mouse hot plate model and formalin test (PubMed:33610632). Expressed by the venom duct. The cysteine framework is I (CC-C-C). Alpha4/7 pattern. Possibly amidated at Cys-64. Belongs to the conotoxin A superfamily. The C-terminal Gly suggests that the peptide is amidated and is one residue shorter (residues 49-64) than that synthesized by Qiang et al., 2021.