The enzyme catalyzes the hydrolysis of N-carbamoyl-D-amino acids to the corresponding which are useful intermediates in the preparation of beta-lactam antibiotics. Industrial production of beta-lactam antibiotics is now being developed using this enzyme. an N-carbamoyl-D-amino acid + 2 H(+) + H2O = a D-alpha-amino acid + CO2 + NH4(+) Homotetramer.