Catalyzes the oxidation of pyridoxine 5'-phosphate (PNP) into pyridoxal 5'-phosphate (PLP). Unlike many PNPOx enzymes, Rv2607 does not recognize pyridoxamine 5'-phosphate (PMP) as a substrate. O2 + pyridoxine 5'-phosphate = H2O2 + pyridoxal 5'-phosphate Appears to bind only one FMN molecule per homodimer. kcat is 0.01 sec(-1). Cofactor metabolism; pyridoxal 5'-phosphate salvage; pyridoxal 5'-phosphate from pyridoxine 5'-phosphate: step 1/1. Homodimer. The electron density for the FMN cofactor is weak in the crystal structure (PDB 2A2J), which does not allow FMN to be definitively placed in the M.tuberculosis PNPOx active site, but the residues known to interact with FMN in the E.coli and human PNPOx enzymes are found to be conserved. Belongs to the pyridoxamine 5'-phosphate oxidase family.