Involved in the oxidation of the plant hormone jasmonoyl-L-isoleucine (JA-Ile), a bioactive phytohormone of the jasmonate-mediated signaling pathway. Converts 12-hydroxy-JA-Ile (12OH-JA-Ile) to the carboxy-derivative 12COOH-JA-Ile (PubMed:17868380, PubMed:22215670, PubMed:26164240). Exerts negative feedback control on JA-Ile levels and plays a role in attenuation of jasmonate responses (PubMed:22215670). Functions also as in-chain fatty acids hydroxylase in vitro (PubMed:17868380). Catalyzes the hydroxylation of 12-hydroxy-jasmonoyl-L-phenylalanine (12OH-JA-Phe) in vitro. Converts 12OH-JA-Phe to the carboxy-derivative 12COOH-JA-Phe (PubMed:26164240). a 12-hydroxyjasmonyl-L-alpha-amino acid + 2 O2 + 2 reduced [NADPH--hemoprotein reductase] = a 12-hydroxy-12-oxojasmonyl-L-alpha-amino acid + 3 H(+) + 3 H2O + 2 oxidized [NADPH--hemoprotein reductase] Induced by methyl jasmonate and wounding. No visible phenotype under normal growth conditions, but mutant plants accumulate high amounts of 12COOH-JA-Ile in response to wounding. Belongs to the cytochrome P450 family.