dTDP-glucose 4,6-dehydratase involved in the generation of the deoxysugar in the novobiocin biosynthesis pathway, an aminocoumarin family antibiotic that targets bacterial DNA gyrases. dTDP-alpha-D-glucose = dTDP-4-dehydro-6-deoxy-alpha-D-glucose + H2O Binds 1 NAD(+) per subunit. Antibiotic biosynthesis; novobiocin biosynthesis. Homodimer. Belongs to the NAD(P)-dependent epimerase/dehydratase family. dTDP-glucose dehydratase subfamily.