Glycosyltransferase involved in the biosynthesis of neomycin by mediating conversion of 2-deoxystreptamine (2-DOS) to 2'-N-acetylparomamine using UDP-alpha-D-glucosamine as sugar donor. 2-deoxystreptamine + UDP-N-acetyl-alpha-D-glucosamine = 2'-N-acetylparomamine + H(+) + UDP Antibiotic biosynthesis; neomycin biosynthesis. Belongs to the glycosyltransferase group 1 family. Glycosyltransferase 4 subfamily. Truncated N-terminus.