Involved in 100S ribosome formation from 70S ribosomes; 100S ribosomes are probably translationally inactive (PubMed:25422304). Ribosome hibernation may be used by the cell to decrease overall energy consumption under nutrient-limiting conditions (PubMed:26324267). Unlike E.coli, 100S ribosomes are present from mid-exponential growth, peak during the transition from log to stationary phase and then decrease (PubMed:25422304). Interacts with 100S ribosomes (PubMed:25422304). Transcribed early in log-phase growth, peaks at log to stationary phase transition (PubMed:25422304). Repressed by growth in the presence of glucose, which also decreases 100S ribosome content (PubMed:25422304). Induced by growth in ethanol, NaCl, and 46 degrees Celsius; stress respones are under control of the sigma-B factor (sigB) (PubMed:25422304). Loss of 100S ribosomes. Shows no change in growth in pure culture after 13 days, however performs very poorly (about 1000-fold decrease) in competition with wild-type (PubMed:25422304). About 5-fold less virulent than wild-type upon oral or tail vein injection of BALB/c mice (PubMed:25422304). Sensitive to aminoglycoside antibiotics in stationary phase; sensitivity is suppressed by transcription and translation inhibitors or by disrupting the proton motive force (PubMed:26324267). Stationary phase cells have 5-fold higher than normal levels of ATP and NADH and accumulates higher intracellular levels of gentamicin (PubMed:26324267). Aminoglycosides such as gentamicin bind to ribosomes and interfere with translation. Belongs to the HPF/YfiA ribosome-associated protein family. Long HPF subfamily.