Alpha-conotoxins act on postsynaptic membranes, they bind to the nicotinic acetylcholine receptors (nAChR) and thus inhibit them. This toxin blocks the rat muscle nAChRs alpha-1-beta-1-gamma-delta (CHRNA1-CHRNB1-CHRNG-CHRND) (IC(50)=5.7 nM) and the rat neuronal nAChR alpha-3-beta-2/CHRNA3-CHRNB2 (IC(50)=2060 nM) (PubMed:29857567). In vivo, intramuscular injection into zebrafish produces rapid flaccid paralysis (PubMed:29857567). Expressed by the venom duct. The cysteine framework is I (CC-C-C). Alpha3/5 pattern. PD(50) is 110 ug/kg into zebrafish. This toxin does not show effect on neuronal nAChR alpha-4-beta-2/CHRNA4-CHRNB2 and alpha-7/CHRNA7 (when tested at 10 uM). Belongs to the conotoxin A superfamily.