Catalyzes the hydrolysis of acyl-CoAs into free fatty acids and coenzyme A (CoASH), regulating their respective intracellular levels (PubMed:7906114). Preferentially hydrolyzes palmitoyl-CoA, but has a broad specificity acting on other fatty acyl-CoAs with chain-lengths of C8-C18 (PubMed:7906114). May play an important physiological function in brain (PubMed:7906114). H2O + hexadecanoyl-CoA = CoA + H(+) + hexadecanoate dodecanoyl-CoA + H2O = CoA + dodecanoate + H(+) H2O + tetradecanoyl-CoA = CoA + H(+) + tetradecanoate decanoyl-CoA + H2O = CoA + decanoate + H(+) H2O + octanoyl-CoA = CoA + H(+) + octanoate H2O + octadecanoyl-CoA = CoA + H(+) + octadecanoate (9Z)-octadecenoyl-CoA + H2O = (9Z)-octadecenoate + CoA + H(+) Lipid metabolism; fatty acid metabolism. Homohexamer. Isoform 1 is expressed constitutively in brain and testis. Isoform 2 is induced in liver by treatment with the peroxisome proliferator. Both HotDog ACOT-type hydrolase domains are required for efficient activity. The N-terminus is blocked. These proteins have been named according to their molecular weight (see PubMed:11755680): BACH (also called CTE-IIa and CTE-II), LACH1 (also called MTE-II) and ACT (also called CTE-IIb). It is unknown whether ACT corresponds to another isoform.