This toxin inhibits numerous potassium channels: shaker (Ki=227 nM), Kv1.2/KCNA2 (nanomolar range), Kv1.3/KCNA3 (nanomolar range), Kv1.5/KCNA5 (Kd>100 nM), Kv1.6/KCNA6 (Ki=22 nM), KCa1.1/KCNMA1 (IC(50)=5.9 nM). It blocks channel activity by a simple bimolecular inhibition process. It also shows a weak interaction with nicotinic acetylcholine receptors (nAChR), suggesting it may weakly inhibit it (PubMed:31276191). It also exhibits pH-specific antimicrobial activities against bacteria (B.subtilis, E.coli and S.aureus) and the fungus C.albicans (PubMed:15118082). Expressed by the venom gland. Has the structural arrangement of an alpha-helix connected to a beta-sheet by disulfide bonds (CSalpha/beta). This toxin does not or very weakly inhibits Kv1.1/KCNA1, Kv2.1/KCNB1 (Ki>2 uM), Kv3.1/KCNC1 (Kd>1 uM), Kv11.1/KCNH2, KCa2.1/KCNN1 (IC(50)> 1 uM), KCa2.2/ KCNN2 (IC(50)> 1 uM), KCa2.3/ KCNN3 (IC(50)> 1 uM) (PubMed:12527813, PubMed:12719233, PubMed:20007782, PubMed:7517498, PubMed:8204618). Does not inhibit the growth of Gram-positive and Gram-negative bacteria (PubMed:26817841). Belongs to the short scorpion toxin superfamily. Potassium channel inhibitor family. Alpha-KTx 01 subfamily.