Has lipid A 3-O-deacylase activity. Hydrolyzes the ester bond at the 3 position of lipid A, a bioactive component of lipopolysaccharide (LPS), thereby releasing the primary fatty acyl moiety. a 3-(acyloxy)acyl derivative of bacterial toxin + H2O = 3-hydroxyacyl derivative of bacterial toxin + a fatty acid + H(+) Homodimer. Consists mainly of an outer membrane-spanning beta-barrel formed by beta-strands both N- and C-terminus residing in the periplasm. Increase in relative abundance of 3-OH C10 in lipid A. The most abundant ion detected in lipid A structure contains the addition of a 3-OH C10 acyl chain and a second phosphate group compared to the most abundant ion observed from wild-type lipid A. Belongs to the PagL family.