Strongly resembles a botulinum-type toxin, with the appropriate domains and residues to have proteolytic function, although its C-terminus (which binds to a eukaryotic host cell) is different enough from clostrial botulinum toxins that it might bind another cell target (PubMed:29323697). Might be a precursor of a toxin that binds to an unknown eukaryotic cell receptor(s), and be taken up into the host cell via the endocytic pathway. When the pH of the putative toxin-containing endosome drops a structural rearrangement occurs so that the N-terminus of the heavy chain forms pores that allows the light chain to translocate into the cytosol. Once in the cytosol the disulfide bond linking the 2 subunits is reduced and light chain cleaves its target protein (By similarity). Limited hydrolysis of proteins of the neuroexocytosis apparatus, synaptobrevins, SNAP25 or syntaxin. No detected action on small molecule substrates. Binds 1 zinc ion per subunit (By similarity). Might be a disulfide-linked heterodimer of a light chain (LC) and heavy chain (HC). The light chain (LC) has protease activity. HC has 3 functional domains; the translocation domain (TD) and the receptor-binding domain (RBD) which is further subdivided into N- and C-terminal domains (N-RBD and C-RBD). The N-terminus of the TD wraps an extended belt around the perimeter of the light chain, protecting Zn(2+) in the active site and may be a pseudosubstrate inhibitor which serves as an intramolecular chaperone for the LC prior to its translocation into the host cytosol. The RBD binds transiently exposed coreceptors on the host presynaptic cell membrane. There are seven antigenically distinct forms of botulinum neurotoxin: Types A, B, C, D, E, F, and G; this protein has not been tested seriologically. New subtypes are quite frequent. This strain was isolated from cow feces in South Carolina, USA, but whether the cattle were infected with botulism is not known. This locus includes a number of other genes usually associated with the botulinum neurotoxin cluster in Clostridia (botR, hemagglutinin genes and p47 proteins), which might permit the toxin to survive in a host. Belongs to the peptidase M27 family.