DIG2 and DIG1 are negative regulators of the filamentation and pheromone induced mating program. DIG1 and DIG2 inhibit the transcriptional activity of STE12 by direct protein-protein interaction. DIG2 binds to the DNA binding domain (DBD) of STE12 and thus inhibits transcription when overexpressed. Forms a complex with DIG1, STE12 and either FUS3 or KSS1. The interaction of FUS3 with STE12 depends on the presence of both DIG1 and DIG2. STE12 is lost from FUS3/DIG1/DIG2 complex after pheromone treatment. DIG1 and DIG2 have also been reported to interact with CLN1 and CLN2. By pheromone. Phosphorylated by FUS3 and KSS1, in a pheromone-stimulated manner. Present with 1310 molecules/cell in log phase SD medium.