DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair. Recruits cellular topoisomerase II to sites of viral replication and assembly. ATP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + diphosphate. Interacts with host TOP2A and TOP2B. Found in sites viral of replication and assembly. Belongs to the ATP-dependent DNA ligase family.