Protein-lysine palmitoyltransferase that catalyzes palmitoylation of the protoxin (CyaA) at two internal lysine residues, thereby converting it to the active toxin (PubMed:7939682, PubMed:8973351, PubMed:33011179, PubMed:32461253). Has strong preference for palmitoyl-CoA (hexadecanoyl-CoA) substrate, compared to other acyl-CoA substrates such as myristoyl-CoA (tetradecanoyl-CoA) (PubMed:33011179, PubMed:32461253). hexadecanoyl-[ACP] + L-lysyl-[protein] = H(+) + holo-[ACP] + N(6)-hexadecanoyl-L-lysyl-[protein] (9Z)-hexadecenoyl-[ACP] + L-lysyl-[protein] = H(+) + holo-[ACP] + N(6)-[(9Z)-hexadecenoyl]-L-lysyl-[protein] Homodimer. Belongs to the RTX toxin acyltransferase family.