During neurogenesis, plays an essential role in axonal guidance and outgrowth by regulating the polarization of both microtubule and actin cytoskeletons (PubMed:1468626, PubMed:11493519, PubMed:16236031, PubMed:22101643, PubMed:25358863, PubMed:30045855). Establishes the asymmetry of axonal and dendrite microtubules and the polarized sorting of neuronal proteins (PubMed:22101643). This is achieved in part by regulating the localization of kinesin-like protein unc-104 (PubMed:22101643). In neurons without a distal microtubule-organizing center (MTOC), also controls the organization of microtubules in dendrites (PubMed:30254025). During the dorso-ventral axonal guidance and outgrowth of VD neurons, required downstream of Rac GTPases ced-10 and mig-2 to inhibit growth cone filopodial protrusion mediated by the unc-6/netrin receptor unc-40-unc-5 (PubMed:25371370). Specifically, regulates growth cone filopodial protrusion polarity, and thus migration, by promoting F-actin polarization and by restricting plus-end microtubule accumulation in the growth cone (PubMed:30045855). Probably downstream of mab-20/Sema2a and mab-20 receptor plx-2, regulates the guidance of DD/VD neuron axons by modulating fln-1 interaction with F-actin which results in the remodeling of the actin cytoskeleton (PubMed:25358863). In hermaphrodites, involved in sex myoblast (SM) migration by regulating the gonad-dependent repulsion of SMs (PubMed:10993679). In neurons, required for the polarized sorting of axonal proteins (PubMed:22101643). In PLM neuron, regulates innexin unc-9 gap junction turnover by suppressing unc-9 transport out of gap junctions (PubMed:27015090). Plays a role in locomotion and egg-laying (PubMed:22101643). In PLM neuron, regulates innexin unc-9 gap junction turnover by suppressing unc-9 transport out of gap junctions. In PLM neuron, regulates innexin unc-9 gap junction turnover by suppressing unc-9 transport out of gap junctions. Isoform a: Probable monomer (PubMed:16236031). Isoform b: Probable homodimer (PubMed:16236031). Isoform c: Probable homodimer (PubMed:16236031). Probable heterodimer composed of isoform b and isoform c (PubMed:16236031). Interacts with unc-14 and kinesin-1 motor complex light chain klc-1; both interactions regulate unc-33 neurite localization (PubMed:16236031). Interacts with fln-1 (via calponin-homology (CH) domains and filamin repeat 18-19) (PubMed:25358863). Isoform c: Interacts with vab-8 isoform a (PubMed:27015090). Expressed in ventral cord and nerve ring (at protein level) (PubMed:16236031). Isoform a: Expressed in nerve ring (at protein level) (PubMed:16236031, PubMed:22101643). Expressed in the nervous system, two amphid socket cells and weakly in non-neuronal pharyngeal cells (PubMed:11493519). Expressed in L1 larval stage and in adults (at protein level) (PubMed:16236031). Isoform a: Expressed in L1 larval stage and in adults (at protein level) (PubMed:16236031). Belongs to the metallo-dependent hydrolases superfamily. Hydantoinase/dihydropyrimidinase family. Lacks most of the conserved residues that are essential for binding the metal cofactor and hence for dihydropyrimidinase activity. Its enzyme activity is therefore unsure.