Involved in de novo bacterial ceramide synthesis (PubMed:33063925, PubMed:34969973). Catalyzes the condensation of 3-oxosphinganine with an acyl-CoA to generate oxidized ceramides (PubMed:34969973). Can use acyl-CoA substrates ranging from C8 to C24, with highest in vitro activity with C14 and very little activity with acyl-CoA thioesters of 18 carbons or longer (PubMed:34969973). May have a preference for monounsaturated acyl-CoA substrates, as it has a threefold greater preference for C16:1-CoA over C16:0-CoA as a substrate in vitro (PubMed:34969973). 3-oxosphinganine + a fatty acyl-CoA = CoA + H(+) + N-acyl-3-oxosphinganine 3-oxosphinganine + tetradecanoyl-CoA = CoA + H(+) + N-tetradecanoyl-3-oxosphinganine 3-oxosphinganine + hexadecanoyl-CoA = CoA + H(+) + N-hexadecanoyl-3-oxosphinganine (9Z)-hexadecenoyl-CoA + 3-oxosphinganine = CoA + H(+) + N-(9Z-hexadecenoyl)-3-oxosphinganine 3-oxosphinganine + octanoyl-CoA = CoA + H(+) + N-octanoyl-3-oxosphinganine 3-oxosphinganine + decanoyl-CoA = CoA + H(+) + N-decanoyl-3-oxosphinganine 3-oxosphinganine + dodecanoyl-CoA = CoA + H(+) + N-dodecanoyl-3-oxosphinganine 3-oxosphinganine + octadecanoyl-CoA = CoA + H(+) + N-octadecanoyl-3-oxosphinganine 3-oxosphinganine + eicosanoyl-CoA = CoA + H(+) + N-eicosanoyl-3-oxosphinganine 3-oxosphinganine + docosanoyl-CoA = CoA + H(+) + N-docosanoyl-3-ketodihydrosphingosine 3-oxosphinganine + tetracosanoyl-CoA = CoA + H(+) + N-tetracosanoyl-3-oxosphinganine Lipid metabolism; sphingolipid metabolism. Deletion mutant cannot form bacterial ceramide (PubMed:33063925, PubMed:34969973). Mutants are impaired in growth at elevated temperatures but are resistant towards the antibiotic polymyxin B (PubMed:33063925). The bacterial ceramide synthesis pathway operates in a different order from that in eukaryotes. Furthermore, phylogenetic analyses support the hypothesis that the bacterial and eukaryotic ceramide pathways evolved independently.