(2R)-2-phosphoglycerate = H2O + phosphoenolpyruvate Mg(2+) is required for catalysis and for stabilizing the dimer. Optimum pH is 7.5-8.5 for the forward reaction (PEP formation) and 6.0-7.5 for the reverse reaction (2-PGA formation). Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5. Highly expressed in young roots, young siliques, and shoot apex. Lowly expressed in young leaves, stems and cotyledons. Distorted trichomes and reduced numbers of root hairs. Belongs to the enolase family.