Potentially capable of cleaving the specific glycoside linkages in the process of mucin degradation in human intestinal tract (Probable). Hydrolyzes chromogenic substrates pNP-beta-GlcNAc with high activity and pNP-beta-GalNAc to a lesser extent, but not pNP-beta-glucose or pNP-beta-galactose (PubMed:32819592). Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides. Inhibited strongly by Cu(2+), Zn(2+), Cd(2+) and Ni(2+) ions. No effect on activity with Na(+), Li(+), K(+), Ca(2+), Mg(2+) or Mn(2+) ions. kcat is 51.39 sec(-1) and kcat/KM is 1.73 M(-1)sec(-1) with pNP-GlcNAc as substrate. Optimum pH is approximately 7. Stable activity from pH 4.5 to 8.5. Retains more than 30% of activity at pH 9.0. Highest activity at 45 degrees Celsius. Has about 40% of activity at 55 degrees Celsius. Belongs to the glycosyl hydrolase 20 family.