Recognizes and binds in the vacant E-site of ribosomes stalled by some peptidyltransferase center (PTC)-targeting antibiotics. Makes contact with the PTC and both ribosomal subunits. Induces conformational changes in the P-site, which allows it to dislodge the antibiotic from its PTC binding site. Binds to ribosomes either directly following translation initation or subsequent to E tRNA release during elongation (PubMed:30126986). Involved in resistance to a narrow spectrum of antibiotics (the streptogramin A antibiotic virginiamycin M, the lincosamide antibiotic lincomycin and the pleuromutilin antibiotic tiamulin) (PubMed:16109936, PubMed:30126986). Binds within the E-site of the 70S ribosome, where it contacts ribosomal proteins L1, L5, L33-1, S7, S11, the 16 and 23S rRNAs and the acceptor arm of the P-site tRNA. Does not stably associate with ribosomes. Expressed during all growth phases; expression is higher during early log phase and decreases as growth continues. Expression is dramatically increased in the presence of antibiotics virginiamycin M and lincomycin. A monocistronic operon. The antibiotic resistance domain (ARD) is packed between the 23S rRNA and the acceptor arm of the P-site tRNA and inserts into the peptidyltransferase center (PTC). The C-terminal extension (CTE) contacts the small ribosomal subunit, positioned in the Shine-Dalgarno-anti-Shine-Dalgarno cavity. Hypersensitivity toward the antibiotics virginiamycin M and lincomycin, mildly increased sensitivity to erythromycin, clindamycin and oleandomycin (PubMed:16109936). Hypersensitivity toward the antibiotics virginiamycin M, lincomycin and tiamulin, in this study has no effect on sensitivity to erythromycin, chloramphenicol or linezolid (PubMed:30126986). Belongs to the ABC transporter superfamily. ABCF family. ARE2 subfamily.