Required for normal sprouting angiogenesis and normal embryonic development of intersegmental vessels (ISV) (PubMed:21520329, PubMed:27484901). Required for normal function of the glomerular filtration barrier (PubMed:28814510). Required for normal axon outgrowth on embryonic motor neurons at the level of the horizontal myoseptum. Required for normal expression of notch1b, suggesting that its functions in angiogenesis and neuron outgrowth are due to decreased expression of notch1b (PubMed:27484901). Plays a role in actin cytoskeleton rearrangement (By similarity). Detected on podocyte foot processes. Detected in larvae (at protein level) (PubMed:28814510). First detected in the dorsal region of the embryo at 15 hpf. Detected along the ventral edge of the neural tube from 22 to 32 hpf. Highly expressed in retina, cranial ganglia, pectoral fin, brain and spinal cord at 48 hpf (PubMed:21520329). Detected in primary motor neurons along the ventral edge of the neuron tube at 24 hpf (PubMed:27484901). Sequence analysis combined with the expression of constructs corresponding each to two or three adjacent TSP type-1 domains suggests the presence of 21 TSP type-1 domains; not all of these are detected by standard bioinformatic tools. Extensively N-glycosylated. Morpholino knockdown of the protein impairs normal embryonic development of intersegmental vessels (ISV) (PubMed:21520329, PubMed:27484901). Morpholino knockdown of the protein causes malformation of the glomerular tufts and a reduction in the number of podocytes per glomerular tuft, and causes pericardial edema in many cases, probably due to impaired function of the glomerular filtration barrier (PubMed:28814510). Morpholino knockdown of the protein causes loss of the parachordal chain in the developing vasculature and absence of laterally projecting motor axons at the level of the horizontal myoseptum (PubMed:27484901). Contaminating sequence. Potential poly-A sequence.