This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA. dUTP + H2O = diphosphate + dUMP + H(+) kcat is 5.15 sec(-1). Pyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP (dUTP route): step 2/2. Homotrimer. The C-terminal 11 residues (motif 5) is important for function; its removal by trypsin decreases kcat about 40-fold with a less than 2-fold increase in KM for dUTP. Belongs to the dUTPase family. Truncated N-terminus. Truncated N-terminus. Truncated N-terminus. Truncated N-terminus. Truncated N-terminus.