Catalyzes the transamination of glyoxylate to glycine and contributes to the glyoxylate detoxification. Catalyzes the transamination between L-serine and pyruvate and weakly contributes to gluconeogenesis from the L-serine metabolism. L-serine + pyruvate = 3-hydroxypyruvate + L-alanine glyoxylate + L-alanine = glycine + pyruvate Homodimer. Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. The intracellular compartmentalization of AGTX in mammalian hepatocytes is species dependent. In human and rabbit, AGTX is peroxisomal. In new world monkeys (marmoset) and rodents (rat and mouse), it is distributed approximately evenly between peroxisomes and mitochondria. In carnivores, like cat, the great majority of the enzyme is mitochondrial with only a small proportion being peroxisomal.