Thought to be involved during neural development in axonal navigation at the ventral midline of the neural tube (By similarity). In spinal cord development plays a role in guiding commissural axons probably by preventing premature sensitivity to Slit proteins thus inhibiting Slit signaling through ROBO1 (By similarity). Required for hindbrain axon midline crossing (PubMed:15105459). Probably interacts with SLIT2. The disease is caused by variants affecting the gene represented in this entry. Belongs to the immunoglobulin superfamily. ROBO family.