Hydrolyzes ester substrates carbon chain lengths ranging from C2 to C14 (PubMed:25224799). In vitro, acetate (C2), butyrate (C4) and caprylate (C6) are hydrolyzed with high efficiency. Has lower activity against laurate (C12), myristate (C14) and caproate (C8), and weak activity against palmitate (C16) (PubMed:25224799). a fatty acid ester + H2O = a fatty acid + an aliphatic alcohol + H(+) an acetyl ester + H2O = acetate + an aliphatic alcohol + H(+) a butanoate ester + H2O = an aliphatic alcohol + butanoate + H(+) a hexanoate ester + H2O = an aliphatic alcohol + H(+) + hexanoate a dodecanoate ester + H2O = an aliphatic alcohol + dodecanoate + H(+) a tetradecanoate ester + H2O = an aliphatic alcohol + H(+) + tetradecanoate an octanoate ester + H2O = an aliphatic alcohol + H(+) + octanoate Optimum pH is 8.0. Optimum temperature is 38 degrees Celsius. Probably anchored in the cell wall and cell membrane via an N-terminal transmembrane helix. Remains active in dormant M.tuberculosis. Belongs to the RsiV family.