Involved in the regulation of the intracellular balance of NAD and NADP, and is a key enzyme in the biosynthesis of NADP. Catalyzes specifically the phosphorylation on 2'-hydroxyl of the adenosine moiety of NAD to yield NADP. It can use ATP and other nucleoside triphosphates (GTP, UTP) as well as inorganic polyphosphate (poly(P)) as a source of phosphorus. ATP + NAD(+) = ADP + H(+) + NADP(+) Divalent metal ions. Both Ca(2+) and Mn(2+) ions are more effective activators than zinc, cobalt, copper and magnesium ions at low concentrations. Allosterically inhibited by NADP and activated by quinolinic acid. Strongly inhibited by HgCl(2). kcat is 8 sec(-1) for kinase activity with NAD (at pH 7.8 and 25 degrees Celsius). Optimum pH is 9. Optimum temperature is 35 degrees Celsius. Half of the activity is lost after treatment at 40 degrees Celsius for 15 minutes. Homodimer. Belongs to the NAD kinase family.