Factor that stimulates resuscitation of dormant cells. Has peptidoglycan (PG) hydrolytic activity. Active in the pM concentration range. Has little to no effect on actively-growing cells. PG fragments could either directly activate the resuscitation pathway of dormant bacteria or serve as a substrate for endogenous Rpf, resulting in low molecular weight products with resuscitation activity (By similarity). Plays a role in reactivating bacteria from chronic tuberculosis (TB) in mice. In infected C57BL/6 mice levels remains constant following reactivation of TB (induced by the nitric oxide synthase inhibitor aminoguanidine) for 4 weeks then decreases over 10-fold until at least 11 weeks. No difference in chronic C57BL/6 mouse infection, but infected mice show delayed reactivation of TB (induced by aminoguanidine, a nitric oxide synthase inhibitor) compared to mice infected with wild-type bacteria. Bacterial loads in lung, liver, and spleen are similar during chronic infection, but increase more slowly in the disrupted mutant after reactivation. Pulmonary B-cell responses are reduced in mice infected with the disrupted strain at advanced stages of TB reactivation. While disease is delayed, it does eventually occur, suggesting other the Rpfs compensate. Belongs to the transglycosylase family. Rpf subfamily.