L-nicotine is used as a growth substrate. Plays a role in nicotine catabolism by cleaving a C-C bond in 2,6-dihydroxypseudooxynicotine. 2,6-dihydroxypseudooxynicotine + H2O = 2,6-dihydroxypyridine + 4-(methylamino)butanoate + H(+) Optimum pH is 7.5. Alkaloid degradation; nicotine degradation; 2,6-dihydroxypyridine and 4-(methylamino)butanoate from 6-hydroxypseudooxynicotine: step 2/2. Homodimer. Belongs to the AB hydrolase superfamily.