Plays a role in the formation of functional neural circuits from the early stages of synapse formation. Has a role in the development of CNS functions involved in locomotor activity. Expressed in PCC neurons and neuroblasts in the procephalic neurogenic region in the central nervous system. Expressed in nerves running along the surface of the ventral ganglion and that extend to the peripheral tissues. Also expressed in motor nerves and boutons of certain muscles, in particular of muscle 8 in the A2 and posterior abdominal segments of young adult flies. In the brain, expressed in both the neuropiles and cortical regions except the retinular cells and laminar neuropile. Most abundant during and/or after neuronal differentiation and during cell specification or axogenesis. Required during pupation for normal motor function development. Flies are unable to jump or fly and walk slowly with an unstable gait. Occasionally they exhibit body and wing tremors while standing or walking.