Dual specificity enzyme that catalyzes the synthesis of pseudouridine from uracil-2604 in 23S ribosomal RNA and from uracil-35 in the anticodon of tRNA(Tyr) (PubMed:11720289, PubMed:27551044). Can, to a small extent, also react with uracil-2605 (PubMed:11720289). uridine(2604) in 23S rRNA = pseudouridine(2604) in 23S rRNA uridine(35) in tRNA(Tyr) = pseudouridine(35) in tRNA(Tyr) Monomer. Belongs to the pseudouridine synthase RsuA family.