Membrane-perturbating peptide with multiple activities (PubMed:34302796). It is insecticidal, since it induces contractile paralysis in insects (L.cuprina) during several hours, and death after 24 hours (PubMed:34302796). It shows antibacterial activity with higher activity against Gram-positive than Gram-negative bacteria (PubMed:34302796). It is also antiparasitic, since it potently inhibits the larval development of the major pathogenic nematode of ruminants (H.contortus, IC(50)=5.1 uM), but fails to reduce the motility of adult males of the other nematode B.malayi (PubMed:34302796). It also shows cytotoxic activity against HEK293 cells (EC(50)=12-14 uM) and induces hemolysis in human erythrocytes (EC(50)=28.6-48.2 uM) (PubMed:34302796). In addition, it causes an important increase in intracellular calcium concentration on neuronal and epithelial cell lines, which supports a non-specific membrane perturbation mechanism of action (PubMed:34302796). In vivo, it induces pain by intraplantar injection into mice, suggesting a defensive function against vertebrate predators (PubMed:34302796). Adopts an alpha-helical conformation in membrane-mimetic environments. Expressed by the venom gland. Monoisotopic mass. PD(50) is 31.4 nmol/g 1 hour after injection into L.cuprina. LD(50) is 32.6 nmol/g 24 hours after injection into L.cuprina. Belongs to the non-disulfide-bridged peptide (NDBP) superfamily. Medium-length antimicrobial peptide (group 3) family. Ponericin-W subfamily.