Potent inhibitor voltage-gated potassium channels Kv1.3/KCNA3 (Kd=0.65 nM) and Kv1.1/KCNA1 (Kd=41 nM) (PubMed:7517498). It appears to block channel activity by a simple bimolecular inhibition process. Induces a transient period of fast flickering in the channel openings, followed by an almost complete blockade of the channel. Its binding affinity to rat brain synaptosomes is 5-fold higher than this of KTX-3. Binding of the toxin to the channel is associated with significant structural rearrangements in both molecules. It also shows a weak interaction with nicotinic acetylcholine receptors (nAChR), suggesting it may weakly inhibit it (PubMed:31276191). Expressed by the venom gland. Has the structural arrangement of an alpha-helix connected to a beta-sheet by disulfide bonds (CSalpha/beta). Shows weak inhibition of large conductance calcium-activated potassium channels (KCa1.1/KCNMA1) (Ki=1560 nM) (PubMed:20007782). Shows weak or no inhibition on Kv1.2/KCNA2(PubMed:7517498, PubMed:20007782). Shows no effect on Kv1.5/KCNA5 and Kv3.1/KCNC1 (PubMed:7517498). Belongs to the short scorpion toxin superfamily. Potassium channel inhibitor family. Alpha-KTx 03 subfamily.