Enzyme that catalyzes the last step in proline biosynthesis. Proline is synthesized from either glutamate or ornithine; both are converted to pyrroline-5-carboxylate (P5C), and then to proline via pyrroline-5-carboxylate reductases (PYCRs). PYCRL is exclusively linked to the conversion of ornithine to proline. L-proline + NADP(+) = 1-pyrroline-5-carboxylate + 2 H(+) + NADPH L-proline + NAD(+) = 1-pyrroline-5-carboxylate + 2 H(+) + NADH Not inhibited by proline. kcat is 197 sec(-1) for the NADH-dependent reduction of pyrroline-5-carboxylate. kcat is 35 sec(-1) for the NADPH-dependent reduction of pyrroline-5-carboxylate. Amino-acid biosynthesis; L-proline biosynthesis; L-proline from L-glutamate 5-semialdehyde: step 1/1. Homodecamer; composed of 5 homodimers. Belongs to the pyrroline-5-carboxylate reductase family. Extended N-terminus. Chimeric cDNA.