Toxic component of an LXG toxin-immunity module (PubMed:32117125). These modules promote kin selection, mediate competition in biofilms, and drive spatial segregation of different strains, indicating that LXG toxins may help avoid warfare between strains in biofilms (By similarity). The C-terminus (residues 527 to 669) has DNase activity in E.coli and in vitro, and inhibits growth upon expression in E.coli; does not have RNase activity. Acts on supercoiled, genomic, linear and nicked DNA. By 2 hours post-induction cells have an irregular surface, membrane disruption and elongate. All toxic effects in E.coli are neutralized by cognate immunity protein YezG. Binds DNA in the presence and absence of YezG; substrate-binding and immunity protein-binding must occur at distinct sites on the toxin (PubMed:32117125). 10 uM Mn(2+) or 10 mM Mg(2+) are equally active in vitro. Optimum pH is 5.5 for the C-terminal fragment DNase. Optimum temperature is 37 degrees Celsius for the C-terminal fragment DNase. The C-terminal fragment is a homodimer (PubMed:32117125). The C-terminus interacts with cognate immunity protein YezG, probably with 2:2 stoichiometry. The second YezG molecules binds with lower affinity (Probable). Present in detergent-resistant membrane (DRM) fractions that may be equivalent to eukaryotic membrane rafts; these rafts include proteins involved in signaling, molecule trafficking and protein secretion. Delivery to target cells requires the type VII secretion system (T7SS) and YukE. In the N-terminal section; belongs to the LXG family.