Catalyzes the hydrolysis of endogenous amidated lipids like the sleep-inducing lipid oleamide ((9Z)-octadecenamide), the endocannabinoid anandamide (N-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-ethanolamine), as well as other fatty amides, to their corresponding fatty acids, thereby regulating the signaling functions of these molecules (PubMed:10526230, PubMed:12664593). Also catalyzes the hydrolysis of the endocannabinoid 2-arachidonoylglycerol (2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-glycerol) (PubMed:10526230, PubMed:12664593). FAAH cooperates with PM20D1 in the hydrolysis of amino acid-conjugated fatty acids such as N-fatty acyl glycine and N-fatty acyl-L-serine, thereby acting as a physiological regulator of specific subsets of intracellular, but not of extracellular, N-fatty acyl amino acids (By similarity). H2O + N-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-ethanolamine = (5Z,8Z,11Z,14Z)-eicosatetraenoate + ethanolamine (9Z)-octadecenamide + H2O = (9Z)-octadecenoate + NH4(+) 2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-glycerol + H2O = (5Z,8Z,11Z,14Z)-eicosatetraenoate + glycerol + H(+) 1-O-methyl-(5Z,8Z,11Z,14Z)-eicosatetraenoate + H2O = (5Z,8Z,11Z,14Z)-eicosatetraenoate + H(+) + methanol (9Z,12Z,15Z)-octadecatrienamide + H2O = (9Z,12Z,15Z)-octadecatrienoate + NH4(+) (5Z,8Z,11Z,14Z)-eicosatetraenamide + H2O = (5Z,8Z,11Z,14Z)-eicosatetraenoate + NH4(+) (6Z)-octadecenamide + H2O = (6Z)-octadecenoate + NH4(+) (15Z)-tetracosenamide + H2O = (15Z)-tetracosenoate + NH4(+) (8Z,11Z,14Z)-eicosatrienamide + H2O = (8Z,11Z,14Z)-eicosatrienoate + NH4(+) (11Z,14Z,17Z)-eicosatrienamide + H2O = (11Z,14Z,17Z)-eicosatrienoate + NH4(+) (11Z,14Z)-eicosadienamide + H2O = (11Z,14Z)-eicosadienoate + NH4(+) (9Z,12Z)-octadecadienamide + H2O = (9Z,12Z)-octadecadienoate + NH4(+) H2O + tetradecamide = NH4(+) + tetradecanoate H2O + N-(9Z-octadecenoyl) ethanolamine = (9Z)-octadecenoate + ethanolamine H2O + N-(9Z-octadecenoyl)-taurine = (9Z)-octadecenoate + taurine (11Z)-eicosenamide + H2O = (11Z)-eicosenoate + NH4(+) H2O + N-(9Z-hexadecenoyl) ethanolamine = (9Z)-hexadecenoate + ethanolamine H2O + N-octadecanoyl ethanolamine = ethanolamine + octadecanoate H2O + N-docosanoyl-ethanolamine = docosanoate + ethanolamine H2O + N-tetracosanoyl-taurine = taurine + tetracosanoate H2O + N-(15Z-tetracosenoyl)-ethanolamine = (15Z)-tetracosenoate + ethanolamine H2O + N-docosanoyl-taurine = docosanoate + taurine H2O + N-(15Z-tetracosenoyl)-taurine = (15Z)-tetracosenoate + taurine H2O + N-tricosanoyl-taurine = taurine + tricosanoate (9Z)-octadecenoate + glycine = H2O + N-(9Z-octadecenoyl)glycine H2O + N-(5Z,8Z,11Z,14Z)-eicosatetraenoyl-glycine = (5Z,8Z,11Z,14Z)-eicosatetraenoate + glycine H2O + N-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-L-serine = (5Z,8Z,11Z,14Z)-eicosatetraenoate + L-serine inhibited by trifluoromethyl ketone. Homodimer. Seems to be associated with the endoplasmic reticulum and/or Golgi apparatus. Belongs to the amidase family.