Monofunctional, high-specificity fructose-2,6-bisphosphatase, which releases phosphate from the 2-position of fructose 2,6-bisphosphate. Has no detectable 6-phosphofructo-2-kinase activity. beta-D-fructose 2,6-bisphosphate + H2O = beta-D-fructose 6-phosphate + phosphate Inhibited by fructose 6-P, activated by glycerol 3-P. Present with 1680 molecules/cell in log phase SD medium. In the C-terminal section; belongs to the phosphoglycerate mutase family.