Catalyzes the transfer of the phosphoenolpyruvate moiety from enoylpyruvoyl-2-diphospho-5'-guanosine (EPPG) to 7,8-didemethyl-8-hydroxy-5-deazariboflavin (FO) with the formation of dehydro coenzyme F420-0 and GMP. 7,8-didemethyl-8-hydroxy-5-deazariboflavin + enolpyruvoyl-2-diphospho-5'-guanosine = dehydro coenzyme F420-0 + GMP + H(+) Cofactor biosynthesis; coenzyme F420 biosynthesis. Homodimer. Inhibits intracellular mycobacterial growth by inducing apoptosis in infected macrophages. Induces macrophage apoptosis by triggering reactive oxygen species (ROS) production, release of cytochrome c, and activation of caspase-3 via a TLR4-dependent pathway. FbiA-mediated apoptosis may act as a host defense response. Was identified as a high-confidence drug target. Belongs to the CofD family.