ATP-dependent chaperone which uses the energy provided by ATP hydrolysis to generate mechanical force to disassemble protein complexes (PubMed:12006565, PubMed:17646390, PubMed:23185031, PubMed:24371142). Plays an essential role in the cytoplasmic maturation steps of pre-60S ribosomal particles by promoting the release of shuttling protein RLP24 from the pre-ribosomal particles (PubMed:17646390, PubMed:23185031, PubMed:24371142). This step facilitates the subsequent release of other shuttling proteins such as NOG1 and allows the transition of the pre-ribosomal particles to later maturation forms that bind REI1 (PubMed:17646390, PubMed:23185031, PubMed:24371142). Essential for viability (PubMed:8109176, PubMed:24371142). ATP + H2O = ADP + H(+) + phosphate The hexamer is activated by RLP24 during pre-60S ribosomal particle maturation; RLP24 activates ATPase activity of both ATP-binding regions and increases cooperativity between AFG2 subunits (PubMed:23185031). The second ATP-binding region is inhibited by diazaborine; the inhibition requires prior ATP binding specifically to the second ATP-binding region (PubMed:24371142). Homohexamer; ATP binding induces oligomerization (PubMed:12006565, PubMed:23185031). Forms a ring-shaped particle of about 12 nm diameter, that displays 6-fold radial symmetry (PubMed:12006565). Associates with cytoplasmic pre-60S ribosomal particles containing ARX1, ALB1, RLP24 and NOG1 (PubMed:17646390). Binds to pre-60S ribosomal particles soon after their export from the nucleus and is released before REI1 and LSG1 are incorporated into the particles (PubMed:17646390). Hexameric form interacts with RLP24 (via C-terminal); the interaction recruits AFG2 to pre-60S ribosomal particles and promotes AFG2 ATPase activity and RLP24 release from pre-60S ribosomal particles (PubMed:23185031, PubMed:24371142). Interacts (via N-terminus) with nucleoporin NUP116 (via N-terminus); the interaction is required for RLP24 release from pre-60S ribosomal particles (PubMed:23185031). The first ATP-binding region binds ATP with low affinity whereas the second ATP-binding region binds ATP with high affinity (PubMed:12006565). ATP hydrolysis mediated by the second ATP binding region releases RLP24 from pre-60S ribosomal particles whereas the ATP hydrolysis mediated by the first ATP binding region is subsequently required for RLP24 dissociation from AFG2, probably by disassembling AFG2 into monomers (PubMed:23185031, PubMed:24371142). Present with 799 molecules/cell in log phase SD medium. Belongs to the AAA ATPase family. AFG2 subfamily.