As part of the OOEP-KHDC3 scaffold, recruits BLM and TRIM25 to DNA replication forks, thereby promoting the ubiquitination of BLM by TRIM25, enhancing BLM retainment at replication forks and therefore promoting stalled replication fork restart (PubMed:29125140). Positively regulates the homologous recombination-mediated DNA double-strand break (DSB) repair pathway by regulating ATM activation and RAD51 recruitment to DSBs in oocytes (PubMed:29955025). Thereby contributes to oocyte survival and the resumption and completion of meiosis (PubMed:29955025). As a member of the subcortical maternal complex (SCMC), plays an essential role for zygotes to progress beyond the first embryonic cell divisions via regulation of actin dynamics (PubMed:18804437, PubMed:25208553). Required for the formation of F-actin cytoplasmic lattices in oocytes which in turn are responsible for symmetric division of zygotes via the regulation of mitotic spindle formation and positioning (PubMed:25208553). Component of the subcortical maternal complex (SCMC), at least composed of NLRP5, KHDC3, OOEP, and TLE6 (PubMed:18804437, PubMed:28992324). Within the complex, interacts with NLRP5, KHDC3 and TLE6 (PubMed:18804437, PubMed:31575650). The SCMC may facilitate translocation of its components between the nuclear and cytoplasmic compartments (By similarity). As part of the SCMC interacts with the SCMC-associated protein NLRP4F (PubMed:31575650). Forms a scaffold complex with KHDC3/FILIA, and interacts with BLM and TRIM25 at DNA replication forks (PubMed:29125140). In the subcortical cytoplasm of early embryos from the 1-cell to the blastocyst stages (PubMed:18804437, PubMed:25208553). From the 2-cell stage, still detected in the subcortex, but excluded from cell-cell contact regions (PubMed:18804437). Expression largely disappears in blastocysts (PubMed:25208553). Expressed in ovaries, where it is restricted to growing oocytes, with greatest levels in fully grown oocytes. Transcripts first detected at 15.5 dpc and peak 1 week after birth (PubMed:18804437). Transcripts accumulate during oogenesis (PubMed:18804437). During meiotic maturation, the vast majority of the transcripts are degraded and virtually none is detected by 2-cell stage embryogenesis (PubMed:18804437). The protein however persists during preimplantation up to the blastocyst stage (PubMed:18804437). At 2-cell stage, excluded from cell-cell contact regions (PubMed:18804437). Continuous exclusion from these regions during preimplantation development leads to the absence of the protein from the inner cells of the morula and the inner cell mass of the blastocyst (PubMed:18804437). Expressed in ovaries at postnatal day 2 (P2), expression peaks at P10, expression is then slightly decreased at P17 and further decreased at P21 (PubMed:31575650). Induced by etoposide. Contains an atypical KH domain with amino acid changes at critical sites, suggesting that it may not bind RNA. Embryonic death at 2.5 dpc (PubMed:18804437). Progression from embryonic 1- to 2-cell stage delayed 6-8 hours. Less than 20% of the embryos progress beyond 2-cell stage (PubMed:18804437). Embryos form unequal sized blastomeres due to smaller, dysmorphic, and displaced mitotic spindles resulting in asymmetric division (PubMed:25208553). Decrease in thickness of subcortical F-actin in zygotes, thickening of F-actin bundles in the cytoplasm and loss of F-actin cytoplasmic lattices (PubMed:25208553). Decrease in CFL1/Cofilin-1 expression in the subcortex and diffused distribution in the cytoplasm of zygotes (PubMed:25208553). Decrease in expression of the SCMC component ZBED3 in oocytes (PubMed:28992324). Oocytes exhibit greater amounts of DNA damage and show an inability to repair DNA double strand breaks (PubMed:29955025). Increase in oocyte apoptosis upon treatment with the DNA cross-linking agent cisplatin (PubMed:29955025). Increased number of oocytes in primordial and primary follicles in 4 week old mice, however this number is decreased in 16 week old mice (PubMed:29955025). 4 hour delay in reaching 50% germinal vesicle breakdown (GVB) with a 20% decrease in the number of oocytes that complete GVB. 2 hour delay in reaching 50% polar body extrusion (PBE) with a decrease of 20% in the number of oocytes that complete PBE (PubMed:29955025). Belongs to the KHDC1 family.