Peptidyl-tRNA hydrolase involved in the recycling of tRNA-Lys from diacetyl-lysyl-tRNA-Lys and is important for mitochondrial function. an N-acyl-L-alpha-aminoacyl-tRNA + H2O = a tRNA + an N-acyl-L-amino acid + H(+) Present with 339 molecules/cell in log phase SD medium. Belongs to the PTH family.