Potent inhibitor of hNav1.7/SCN9A (IC(50)=16 nM, tested in patch-clamp), and modest inhibitor of Cav2.2/CACNA1B (IC(50)~3 uM on rat and human channel) (PubMed:28880874). It seems also to be potent on hNav1.1/SCN1A (IC(50)=2.18 uM), hNav1.2/SCN2A (IC(50)=0.13 uM), hNav1.7/SCN9A (IC(50)=3.34 uM) and hNav1.8/SCN10A (IC(50)=6.92 uM) (tested in fluorimetric assays, which shows results with lower activity than results obtained by patch-clamp) (PubMed:28880874). This toxin inhibits Cav2.2/CACNA1B by acting near the pore region, but without overlapping the omega-conotoxin binding site (PubMed:28880874). It also seems to interfere with inactivation of Cav2.2/CACNA1B with no apparent effects on the activation gating of the channel (PubMed:28880874). Conversely, at Nav1.7/SCN9A it acts as a typical gating modifier toxin, interacting with one or more of the voltage sensor domains and changing the gating properties of this channel (PubMed:28880874). In vivo, it reversibly paralyzes sheep blowflies (PubMed:28880874). In addition, low doses of this toxin (400 pmol) fully reverse Nav1.7/SCN9A-evoked pain behaviors in mice (without side effects), demonstrating its analgesic potential (PubMed:28880874). Expressed by the venom gland. The presence of a 'disulfide through disulfide knot' structurally defines this protein as a knottin. Monoisotopic mass. PD(50) is 1318 +-58 pmol/g when injected into the ventro-lateral thoracic region of adult sheep blowflies (L.cuprina). Remarkably, all flies fully recovers from the initial paralytic effects induced by this toxin at doses up to 8.17 nmol/g. Has no effect on some calcium channels (Cav1.3/CACNA1D and Cav3.1/CACNA1G) and some sodium channels (Nav1.3/SCN3A, Nav1.4/SCN4A, Nav1.5/SCN5A, and Nav1.6/SCN8A) (IC(50)>30 uM for all channels) (tested in fluorimetric assays) (PubMed:28880874). Belongs to the neurotoxin 10 (Hwtx-1) family. 04 (CcoTx1) subfamily.