As a member of the subcortical maternal complex (SCMC), plays an essential role for zygotes to progress beyond the first embryonic cell divisions via regulation of actin dynamics (PubMed:11062459, PubMed:18804437, PubMed:25208553). Required for the formation of F-actin cytoplasmic lattices (CPL) in oocytes, which in turn are responsible for symmetric division of zygotes via the regulation of mitotic spindle formation and positioning (PubMed:25208553). Required for the localization of cortical granules to the cortex of oocytes, via association with the cortical actin scaffold (PubMed:31118423). Required for cortical actin clearance prior to oocyte exocytosis and prevention of polyspermy (PubMed:31118423). Involved in regulating post-fertilization Ca(2+) release and endoplasmic reticulum storage (ER) storage via regulation of cellular localization (PubMed:24374158). May be involved in the localization of mitochondria to the cytoplasm and perinuclear region in oocytes and early stage embryos, independent of its role in CPL formation (PubMed:22357545). Component of the subcortical maternal complex (SCMC), at least composed of NLRP5, KHDC3, OOEP, and TLE6 (PubMed:18804437, PubMed:28992324, PubMed:31575650). Within the complex, interacts with OOEP, KHDC3 and TLE6 (PubMed:18804437, PubMed:28992324, PubMed:31575650). The SCMC may facilitate translocation of its components between the nuclear and cytoplasmic compartments (By similarity). As part of the SCMC interacts with the SCMC-associated protein ZBED3 (PubMed:28992324). As part of the SCMC interacts with the SCMC-associated protein CFL1/Cofilin-1 (PubMed:25208553). Interacts with PRKCE (By similarity). Interacts with TUBB3 at cytoskeleton microtubules (PubMed:24374158). Located in mitochondria and nucleoli in primary follicle oocytes (PubMed:14670992). Colocalizes with ZBED3 and NLRP4F in the oocyte subcortex (PubMed:25208553, PubMed:28992324, PubMed:31575650). Not detected in oocyte mitochondria in type 2 follicles (PubMed:14670992). In the subcortical cytoplasm of early embryos from the 1-cell to the blastocyst stages (PubMed:14670992, PubMed:25208553). From the 2-cell stage, still detected in the subcortex, but excluded from cell-cell contact regions (PubMed:14670992). Expression largely disappears in blastocysts (PubMed:25208553). Expression is first detected in oocytes of type 3A primary follicles (PubMed:14670992, PubMed:31118423). Transcripts accumulate during oogenesis (PubMed:14670992). Expressed in the cytoplasm of germinal vesicle oocytes before becoming concentrated in the subcortex of metaphase 2 oocytes (PubMed:28992324, PubMed:31118423). During meiotic maturation, the vast majority of the transcripts are degraded and virtually none is detected by 2-cell stage embryogenesis (PubMed:14670992, PubMed:18804437). The protein however persists during preimplantation up to the blastocyst stage (PubMed:14670992, PubMed:18804437). At 2-cell stage, excluded from cell-cell contact regions (PubMed:18804437). Continuous exclusion from these regions during preimplantation development leads to the absence of the protein from the inner cells of the morula and the inner cell mass of the blastocyst (PubMed:18804437). Expressed in ovaries at birth, expression peaks at postnatal day 10 (P10), expression is then decreased at P17 and further decreased at P21 (PubMed:10433232, PubMed:31575650). (Microbial infection) Reduced by T.gondii in the testes and uterus. Phosphorylated by PRKCE. Mice appear phenotypically normal with no apparent defects in ovarian development, oocyte maturation or ovulation (PubMed:14670992, PubMed:22357545). Cortical granules are scattered throughout the cytoplasm in oocytes and ovulated eggs (PubMed:31118423). Reduced abundance of intact MYH9/MyoIIA and MYO5A, the dense cortical actin scaffold remained present prior to exocytosis (PubMed:31118423). Increase in polyspermy in the perivitelline space resulting from a decrease in efficacy of the zona pellucida block. Increase in multiple pronuclei and delay in cortical granule exocytosis and ZP2 cleavage (PubMed:31118423). Embryos form unequal sized blastomeres due to smaller, dysmorphic, and displaced mitotic spindles resulting in asymmetric division (PubMed:25208553). Increase in apoptotic cells in blastomeres which progress beyond the 8-cell stage (PubMed:22357545). Decrease in thickness of subcortical F-actin in zygotes, thickening of F-actin bundles in the cytoplasm and loss of F-actin cytoplasmic lattices (PubMed:25208553). Increase in subcortical mitochondrial clustering of mitochondria in 2-cell embryos (PubMed:22357545). Increase in mitochondria activity, shown by an increase in membrane potential and reactive oxygen species (PubMed:22357545). Decrease in expression of the SCMC-associated protein ZBED3 in the cytoplasm of oocytes (PubMed:28992324). In knockdown mice M2 oocytes show a diffuse ER distribution pattern with a reduced number of cortical ER clusters and random movement of lipid droplets during oocyte maturation (PubMed:24374158). Reduces tubulin localization to the cellular microtubular architecture (PubMed:24374158). Decrease in storage and release of Ca(2+) from the endoplasmic reticulum, and disruption of Ca(2+) oscillations following oocyte fertilization (PubMed:24374158). diffused localization of ITPR1/IP3R-1 throughout the cytoplasm with reduced clustering at the oocyte cortex (PubMed:24374158). Belongs to the NLRP family.