(S)-malate + NAD(+) = CO2 + NADH + pyruvate H(+) + oxaloacetate = CO2 + pyruvate Divalent metal cations. Prefers magnesium or manganese. Non-competitively inhibited by high concentrations of NAD(+) and L-malate. Also inhibited by CoA, acetyl-phosphate, palmitoyl-CoA, and oxaloacetate. Activated by aspartate. At pH 7.2. Optimum pH is 7.2 to 7.5 for L-malate. Homotetramer. Can also use NADP(+) but is more effective with NAD(+). Belongs to the malic enzymes family. Extended N-terminus. The sequence differs from position 433 onward for unknown reasons.