Involved in the biosynthesis of the anthracycline antitumor agent aclacinomycin A. Catalyzes the transfer of the proximal deoxyhexose, L-rhodosamine, from dTDP-beta-L-rhodosamine to the C7-OH of aklavinone aglycone to yield aclacinomycin T (rhodosaminyl-aklavinone). It can also use dTDP-2-deoxy-beta-L-fucose, TDP-2-deoxyfucose, dTDP-4-amino-2-deoxyrhamnose, TDP-L-rhodosamine as sugar donor and epsilon-rhodomycinone as sugar acceptor. aklavinone + dTDP-beta-L-rhodosamine = aclacinomycin T + dTDP + 2 H(+) The activity of AknS is substantially increased by the addition of the accessory protein AknT. kcat is 9.6 sec(-1) for glycosyltransferase activity with aklavinone as sugar acceptor and TDP-L-rhodosamine as sugar donor. kcat is 0.05 sec(-1) for glycosyltransferase activity without AknT and with aklavinone as sugar acceptor and TDP-L-rhodosamine as sugar donor. kcat is 0.17 min(-1) for glycosyltransferase activity with aklavinone as sugar acceptor and dTDP-4-amino-2-deoxyrhamnose as sugar donor. kcat is 0.082 min(-1) for glycosyltransferase activity with epsilon-rhodomycinone as sugar acceptor and TDP-2-deoxyfucose as sugar donor. Belongs to the glycosyltransferase 28 family.