Peroxisomal 2-OH acyl-CoA lyase involved in the cleavage (C1 removal) reaction in the fatty acid alpha-oxydation in a thiamine pyrophosphate (TPP)-dependent manner (By similarity). Involved in the degradation of 3-methyl-branched fatty acids like phytanic acid and the shortening of 2-hydroxy long-chain fatty acids (By similarity). Plays a significant role in the biosynthesis of heptadecanal in the liver (PubMed:29027957). a 2-hydroxy-3-methyl fatty acyl-CoA = a 2-methyl-branched fatty aldehyde + formyl-CoA an (R)-2-hydroxy-long-chain-fatty acyl-CoA = a long-chain fatty aldehyde + formyl-CoA 2-hydroxy-3-methylhexadecanoyl-CoA = 2-methylpentadecanal + formyl-CoA 2-hydroxyoctadecanoyl-CoA = formyl-CoA + heptadecanal 2-hydroxyphytanoyl-CoA = 2,6,10,14-tetramethylpentadecanal + formyl-CoA Binds 1 Mg(2+) ion per subunit. Binds 1 thiamine pyrophosphate per subunit. Lipid metabolism; fatty acid metabolism. Homotetramer. Predominanly expressed in liver. Mice are viable and fertile and show no abnormal phenotype (PubMed:28629946, PubMed:29027957). However, upon dietary administration of phytol, phytanic acid accumulated in tissues, mainly in liver and serum of deficient mice. As a consequence of phytanic acid (or a metabolite) toxicity, deficent mice display a significant weight loss, absence of abdominal white adipose tissue, enlarged and mottled liver and reduced hepatic glycogen and triglyceride (PubMed:28629946). The presence of an other lyase, probably HCL2, can partially compensate for the lost of HCL1 (PubMed:28629946). Belongs to the TPP enzyme family.