ATP-dependent transporter of the ATP-binding cassette (ABC) family that actively extrudes physiological compounds, and xenobiotics from cells. Mediates ATP-dependent transport of endogenous metabolites such as cAMP and cGMP, folic acid and N-lactoyl-amino acids (in vitro). Acts also as a general glutamate conjugate and analog transporter that can limit the brain levels of endogenous metabolites, drugs, and toxins. Confers resistance to the antiviral agent PMEA. Able to transport several anticancer drugs including methotrexate, and nucleotide analogs in vitro, however it does with low affinity, thus the exact role of ABCC5 in mediating resistance still needs to be elucidated. Acts as a heme transporter required for the translocation of cytosolic heme to the secretory pathway (By similarity). May play a role in energy metabolism by regulating the glucagon-like peptide 1 (GLP-1) secretion from enteroendocrine cells (By similarity). ATP + H2O + xenobioticSide 1 = ADP + phosphate + xenobioticSide 2. 3',5'-cyclic GMP(in) + ATP + H2O = 3',5'-cyclic GMP(out) + ADP + H(+) + phosphate 3',5'-cyclic AMP(in) + ATP + H2O = 3',5'-cyclic AMP(out) + ADP + H(+) + phosphate ATP + H2O + N-acetyl-L-aspartyl-L-glutamate(in) = ADP + H(+) + N-acetyl-L-aspartyl-L-glutamate(out) + phosphate ATP + H2O + N-acetyl-L-aspartyl-L-glutamyl-L-glutamate(in) = ADP + H(+) + N-acetyl-L-aspartyl-L-glutamyl-L-glutamate(out) + phosphate ATP + H2O + N-acetyl-L-glutamate(in) = ADP + H(+) + N-acetyl-L-glutamate(out) + phosphate ATP + H2O + N-acetyl-L-aspartate(in) = ADP + H(+) + N-acetyl-L-aspartate(out) + phosphate (2S)-2-[5-amino-1-(beta-D-ribosyl)imidazole-4-carboxamido]succinate(in) + ATP + H2O = (2S)-2-[5-amino-1-(beta-D-ribosyl)imidazole-4-carboxamido]succinate(out) + ADP + H(+) + phosphate ATP + domoate(in) + H2O = ADP + domoate(out) + H(+) + phosphate ATP + beta-citrylglutamate(in) + H2O = ADP + beta-citrylglutamate(out) + H(+) + phosphate ATP + H2O + kainate(in) = ADP + H(+) + kainate(out) + phosphate ATP + H2O + N-[(S)-lactoyl]-L-phenylalanine(in) = ADP + H(+) + N-[(S)-lactoyl]-L-phenylalanine(out) + phosphate ATP + folate(in) + H2O = ADP + folate(out) + H(+) + phosphate In most cells, routes to the basolateral plasma membrane, but in the brain capillary endothelial cells that form the blood-brain barrier, resides in the apical membrane. Although other labs have confirmed the ability of ABCC5 to transport cGMP in an ATP-dependent manner, they obtained a much lower affinity for this substrate. The authors conclude that ABCC5 is a low-affinity cyclic nucleotide transporter and a major function in cGMP excretion is unlikely. Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.