As a member of the subcortical maternal complex (SCMC), plays an essential role for zygotes to progress beyond the first embryonic cell divisions via regulation of actin dynamics (PubMed:18804437, PubMed:25208553). Required for the formation of F-actin cytoplasmic lattices in oocytes which in turn are responsible for symmetric division of zygotes via the regulation of mitotic spindle formation and positioning (PubMed:31575650). Regulates spermatogonia proliferation and cell cycle progression, potentially via regulation of cell cycle regulatory genes such as; CEBPB, CEBPA, CSF3, PCNA, and CDK4 (PubMed:32823735). Suppresses FOXG1/BF-1-mediated transcriptional repression by inhibiting interaction of the transcriptional corepressor TLE1 with FOXG1 which promotes cortical neuron differentiation (PubMed:16314515). Acts as a transcriptional corepressor of NFATC1-mediated gene expression by contributing to PAX6-mediated repression (PubMed:23990468). Homodimers (PubMed:16314515). Component of the subcortical maternal complex (SCMC), at least composed of NLRP5, KHDC3, OOEP, and TLE6 (PubMed:18804437, PubMed:28992324). Within the complex, interacts with NLRP5, KHDC3 and OOEP (PubMed:28992324, PubMed:31575650). The SCMC may facilitate translocation of its components between the nuclear and cytoplasmic compartments (By similarity). As part of the SCMC interacts with the SCMC-associated protein ZBED3 (PubMed:31575650). As part of the SCMC interacts with the SCMC-associated protein NLRP4F (PubMed:31575650). As part of the SCMC interacts with the SCMC-associated protein CFL1/Cofilin-1 (PubMed:25208553). Interacts with FOXG1/BF-1; the interaction inhibits TLE1 interaction with FOXG1/BF-1 (PubMed:16314515). Interacts with NFATC1 (PubMed:23990468). Interacts with PAX6 (PubMed:23990468). In the subcortical cytoplasm of early embryos from the 1-cell to the blastocyst stages (PubMed:18804437, PubMed:25208553). At the 2-cell and morular stage, still detected in the subcortex, but excluded from cell-cell contact regions (PubMed:18804437, PubMed:25208553). Expression largely disappears in blastocysts (PubMed:25208553). Expressed in spermatogonia (at protein level) (PubMed:32823735). Expressed predominantly in ovaries, where it is restricted to growing oocytes, with greatest levels in fully grown oocytes (PubMed:18804437, PubMed:25208553). Expressed predominantly in testis, heart, lung, liver and muscle, and at very low levels in the brain, spleen and kidney (PubMed:11486032). Expressed in bone marrow-derived macrophages and osteoclasts (PubMed:23990468). Transcripts first detected at 15.5 dpc and peak 1 week after birth. Transcripts accumulate during oogenesis (PubMed:18804437). During meiotic maturation, the vast majority of the transcripts are degraded and virtually none is detected by 2-cell stage embryogenesis (PubMed:18804437). The protein however persists during preimplantation up to the blastocyst stage (PubMed:18804437). At 2-cell stage, excluded from cell-cell contact regions. Continuous exclusion from these regions during preimplantation development leads to the absence of the protein from the inner cells of the morula and the inner cell mass of the blastocyst (PubMed:18804437). Expressed in the forebrain, midbrain, ventricular zone and superficial cortical plate at 14.5 dpc (PubMed:16314515). Expressed in cortical progenitors in the forebrain dorsal telencephalon and the midbrain at 15.5 dpc (PubMed:16314515). Expressed in ovaries at postnatal day 2 (P2), expression peaks at P10, expression is then decreased at P17 and further decreased at P21 (PubMed:31575650). Induced by E2A-HLF, a chimeric transcription factor containing the transactivation domain of E2A linked to the DNA-binding and dimerization domain of HLF (PubMed:11486032). Induced during TNFSF11/RANKL-induced osteoclast differentiation (PubMed:23990468). Contrary to other WD repeat Groucho/TLE family members, does not contain any identifiable Q, GP, CcN or SP domains. Only the C-terminal WD-repeat domain is conserved. Females show normal ovarian histology, ovulation and egg morphology, however fail to produce offspring following successful mating (PubMed:25208553). Progression from one- to two-cell embryos is delayed by 4-6 hours and embryos failed to develop into morulae and blastocysts (PubMed:25208553). Embryos form unequal sized blastomeres due to smaller, dysmorphic, and displaced mitotic spindles resulting to asymmetric division (PubMed:25208553). Loss of FMN2-expressing endoplasmic reticulum localization to the mitotic spindle periphery, and incorrect localization of mitochondria to the subcortical region prior to nuclear envelope breakdown in zygotes and oocytes (PubMed:28992324). Extension of the alpha-tubulin pool into the subcortical region following microtubule-organizing center congression in oocytes (PubMed:28992324). Decrease in expression of the SCMC components ZBED3, NLRP5/MATER, KHDC3/FILIA and OOEP/FLOPED in oocytes (PubMed:25208553, PubMed:28992324). Loss of F-actin cytoplasmic lattices in oocytes, zygotes and embryos (PubMed:25208553, PubMed:31575650). Decrease in thickness of subcortical F-actin in zygotes and thickening of F-actin bundles in the cytoplasm is evident (PubMed:25208553). Decrease in CFL1/Cofilin-1 expression in the subcortex, diffused distribution in the cytoplasm of zygotes and decrease in phosphorylated CFL1/Cofilin-1 expression in oocytes and zygotes (PubMed:25208553). Belongs to the WD repeat Groucho/TLE family.