Catalyzes the hydroxylation of the N(6)-(4-aminobutyl)-L-lysine intermediate to form hypusine, an essential post-translational modification only found in mature eIF-5A factor (By similarity). Essential for organismal viability and plays a role in a wide number of important processes such as cell growth and proliferation, and regulates induction of autophagy and protein synthesis. Has a role in eIF-5A-mediated translational control. [eIF5A protein]-deoxyhypusine + AH2 + O2 = [eIF5A protein]-hypusine + A + H2O Binds 2 Fe(2+) ions per subunit. Protein modification; eIF5A hypusination. In larval imaginal disk and Garland cells. Lethality. Mutant clones exhibit bristle loss as well as very small bristles on the thorax. Mutations affect cell and organ size, bromodeoxyuridine incorporation and autophagy. Belongs to the deoxyhypusine hydroxylase family.