Hydrolyzes D-aminoacyl-tRNA into D-amino acid and free tRNA. Broad specificity toward the amino acid, but strict specificity toward the D-isomer. Seems to be required for ethanol tolerance. a D-aminoacyl-tRNA + H2O = a D-alpha-amino acid + a tRNA + H(+) glycyl-tRNA(Ala) + H2O = glycine + H(+) + tRNA(Ala) Binds 2 Zn(2+) ions per subunit. Ubiquitous. Not induced by abscisic acid, paraquat, 1-aminocyclopropane-1-carboxylate, ethanol, salt, sorbitol, cold, heat or low oxygen stresses. Increased sensitivity to acetaldehyde and hypersensitivity to ethanol. Belongs to the DtdA deacylase family. Extended N-terminus.