H2O + L-asparagine = L-aspartate + NH4(+) Homotetramer. Expression is induced by asparagine. B.subtilis contains two L-asparaginase isoenzymes: L-asparaginase I, a low-affinity enzyme located in the cytoplasm, and L-asparaginase II, a high-affinity secreted enzyme. Produced in 2 x SG medium but not in 2 x YT medium. Produced in 2 x YT medium but not in 2 x SG medium. Belongs to the asparaginase 1 family.