Synthesis and degradation of fructose 2,6-bisphosphate. beta-D-fructose 2,6-bisphosphate + H2O = beta-D-fructose 6-phosphate + phosphate ATP + beta-D-fructose 6-phosphate = ADP + beta-D-fructose 2,6-bisphosphate + H(+) Homodimer. Expression is under the control of the RFX1 transcription factor. Present with 3380 molecules/cell in log phase SD medium. In the C-terminal section; belongs to the phosphoglycerate mutase family.