Catalyzes the deacylation of acyl-homoserine lactone (AHL or acyl-HSL), releasing homoserine lactone (HSL) and the corresponding fatty acid. Possesses a specificity for the degradation of long-chain acyl-HSLs (side chains of 11 to 14 carbons in length) (By similarity). an N-acyl-L-homoserine lactone + H2O = a carboxylate + L-homoserine lactone Heterodimer of an alpha subunit and a beta subunit processed from the same precursor. AHL-mediated signaling mediates quorum sensing in many species of Proteobacteria, regulating hundreds of genes, including many that code for extracellular virulence factors. Belongs to the peptidase S45 family.