Converts unsaturated trans-2-enoyl-CoA species ((2E)-enoyl-CoA) to the corresponding 3(S)-3-hydroxyacyl-CoA species through addition of a water molecule to the double bond. Catalyzes the hydration of medium- and short-chained fatty enoyl-CoA thioesters from 4 carbons long (C4) up to C16 (By similarity). Has high substrate specificity for crotonyl-CoA ((2E)-butenoyl-CoA) and moderate specificity for acryloyl-CoA, 3-methylcrotonyl-CoA (3-methyl-(2E)-butenoyl-CoA) and methacrylyl-CoA ((2E)-2-methylpropenoyl-CoA). Can bind tiglyl-CoA (2-methylcrotonoyl-CoA), but hydrates only a small amount of this substrate (By similarity). Plays a key role in the beta-oxidation spiral of short- and medium-chain fatty acid oxidation. At a lower rate than the hydratase reaction, catalyzes the isomerase reaction of trans-3-enoyl-CoA species (such as (3E)-hexenoyl-CoA) to trans-2-enoyl-CoA species (such as (2E)-hexenoyl-CoA), which are subsequently hydrated to 3(S)-3-hydroxyacyl-CoA species (such as (3S)-hydroxyhexanoyl-CoA) (By similarity). a (3S)-3-hydroxyacyl-CoA = a (2E)-enoyl-CoA + H2O a (3E)-enoyl-CoA = a 4-saturated (2E)-enoyl-CoA (3E)-hexenoyl-CoA = (2E)-hexenoyl-CoA (3S)-3-hydroxybutanoyl-CoA = (2E)-butenoyl-CoA + H2O 3-hydroxyisovaleryl-CoA = 3-methyl-(2E)-butenoyl-CoA + H2O 3-hydroxypropanoyl-CoA = acryloyl-CoA + H2O 3-hydroxybutanoyl-CoA = (2E)-butenoyl-CoA + H2O (2E)-2-methylpropenoyl-CoA + H2O = (S)-3-hydroxyisobutanoyl-CoA (3S)-hydroxyhexanoyl-CoA = (2E)-hexenoyl-CoA + H2O (3S)-hydroxydecanoyl-CoA = (2E)-decenoyl-CoA + H2O Lipid metabolism; fatty acid beta-oxidation. Homohexamer; dimer of trimers. Belongs to the enoyl-CoA hydratase/isomerase family.