E3 ubiquitin-protein ligase activity (By similarity). During embryonic and larval development, regulates the pattern of axonal projections of class IV nociceptive sensory neurons (C4da) downstream of netrin receptor fra (PubMed:22084112, PubMed:21338947). Regulates fine-scale topography of C4da axon terminals upon neuronal activity (PubMed:24746793). During eye development, consolidates the attachment of R8 photoreceptor growth cones to the target medulla layer, probably downstream of fra (PubMed:27743477). S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine. Protein modification; protein ubiquitination. Interacts (via fibronectin type-III domain) with pico. Interacts (via SPRY domain) with netrin receptor fra. Colocalizes with fra and pico. Detected at embryonic stages 13-14 (PubMed:22084112). During embryonic and larval development, expressed in the ventral nerve cord (VNC) in a subset of peripheral nervous system neurons including C4da (at protein level) (PubMed:22084112, PubMed:21338947, PubMed:24746793). Induced by neuronal activity in C4da neurons. The fibronectin type-III, SPRY and coil-coil domains are necessary for C4da axon patterning. Defective axonal patterning of terminal processes in class IV nociceptive sensory neurons (C4da), including defective contralateral projections in ddaC and vdaB neurons, during larval development (PubMed:22084112, PubMed:21338947, PubMed:24746793). Defective larval locomotion behavior (PubMed:22084112). Defective targeting of R8 photoreceptor growth cones to the medulla layer during eye development (PubMed:27743477). Belongs to the TRIM/RBCC family.