Glycosyltransferase involved in the biosynthesis of kanamycin by mediating conversion of 2-deoxystreptamine (2-DOS) to 2'-N-acetylparomamine using UDP-alpha-D-glucose as sugar donor. Can also accept UDP-alpha-D-glucosamine, but with a much lower activity compared to UDP-alpha-D-glucose. 2-deoxystreptamine + UDP-N-acetyl-alpha-D-glucosamine = 2'-N-acetylparomamine + H(+) + UDP 2-deoxystreptamine + UDP-alpha-D-glucose = 2'-deamino-2'-hydroxyparomamine + H(+) + UDP Antibiotic biosynthesis; kanamycin biosynthesis. Belongs to the glycosyltransferase group 1 family.