Metallocarboxypeptidase that mediates deglutamylation of tubulin and non-tubulin target proteins (PubMed:25103237). Catalyzes the removal of polyglutamate side chains present on the gamma-carboxyl group of glutamate residues within the C-terminal tail of tubulin protein (PubMed:25103237). Specifically cleaves tubulin long-side-chains, while it is not able to remove the branching point glutamate (PubMed:25103237). Also catalyzes the removal of polyglutamate residues from the carboxy-terminus of non-tubulin proteins such as MYLK (PubMed:25103237). May catalyze the hydrolysis of aspartate from the carboxy-terminus of target proteins (PubMed:25103237). Does not show detyrosinase or deglycylase activities from the carboxy-terminus of target proteins (PubMed:25103237). (L-glutamyl)(n+1)-gamma-L-glutamyl-L-glutamyl-[protein] + H2O = (L-glutamyl)(n)-gamma-L-glutamyl-L-glutamyl-[protein] + L-glutamate Binds 1 zinc ion per subunit. Widely expressed. Expressed abundantly in tissues with m otile cilia such as testis, lung and trachea. Abundantly expressed in pituitary and kidney, moderately expressed in brain, eye, fat, pancreas, stomach, and adrenal. Up-regulated during ciliogenesis. AGBL2 and AGBL3 double knockout mutant mice are viable and display no obvious phenotypic alterations. Show an increase in tubulin and MYLK polyglutamylation in sperm. Belongs to the peptidase M14 family.