The isoenzyme MDH2 may function primarily in the glyoxylate cycle. (S)-malate + NAD(+) = H(+) + NADH + oxaloacetate Homodimer. By acetate as carbon source in the growth medium. Is inactivated by addition of glucose (catabolite inactivation). The Pro/N-degron targets the protein for proteasomal degradation when cells are shifted to glucose-containing growth medium. Targeted for proteasomal degradation when cells are shifted to glucose-containing growth medium. Yeast contains at least 3 malate dehydrogenase isoenzymes: a mitochondrial (MDH1), a cytoplasmic (MDH2) and a peroxisomal (MDH3). Present with 5260 molecules/cell in log phase SD medium. Belongs to the LDH/MDH superfamily. MDH type 1 family.