Cleaves H-Pro-AMC as well as a wide spectrum of amino acid substrates and several peptide substrates without a proline at the N-terminus. Proteases F1, F2 and F3 degrade oligopeptides produced by Tricorn (themselves probably produced by the proteasome) yielding free amino acids. Release of N-terminal proline from a peptide. Part of the tricorn proteolytic complex. Belongs to the peptidase S33 family.