Hydrolyzes (R)-piperazine-2-carboxamide and (R)-piperazine-2-tert-butylcarboxamide with strict R-stereoselectivity. Also active towards beta-alaninamide, piperidine-3-carboxmide, D-glutaminamide and slightly active towards L-glutaminamide and piperidine-4-carboxamide. (R)-piperazine-2-carboxamide + H2O = (R)-piperazine-2-carboxylate + NH4(+) beta-alaninamide + H2O = beta-alanine + NH4(+) Completely inhibited by p-chloromercuribenzoate, N-ethylmaleimide, MnSO(4), MnCl(2), CoCl(2), NiCl(2), CuSO(4), CuCl(2), ZnSO(4), ZnCl(2), AgNO(3), CdCl(2), HgCl(2) and PbCl(2). Partially inhibited by FeCl(3) and Fe(NH(4))(2)(SO(4))(2). Slightly enhanced by dithiothreitol. Unaffected by LiBr, H(2)BO(3), NaCl, MgSO(4), MgCl(2), AlCl(3), KCl, CaCl(2), CrCl(3), RbCl, Na(2)MoO(4), (NH(4))(6)Mo(7)O(24), CsCl and BaCl(2). Unaffected by the chelating agents o-phenanthroline, 8-hydroxyquinoline, enthylenediaminetetraacetic acid and alpha,alpha'-dipyridyl. Not inhibited by the carbonyl reagents hydroxylamine, phenylhydrazine, hydrazine, D,L-penicillamine and D-cycloserine. Not affected by the serine protease inhibitor phenylmethanesulfonyl fluoride, the serine/cysteine protease inhibitor leupeptine or the aspartic protease inhibitor pepstatin. Optimum pH is 8.0. Stable from pH 6.0 to 9.0. Optimum temperature is 45 degrees Celsius, activity decreases rapidly above 45 degrees Celsius possibly due to instability at higher temperatures. Inactivated following 10 minutes incubation at 55 degrees Celsius, and only retains 2.6% of activity after 10 minutes incubation at 50 degrees Celsius. Monomer.