Involved in acetylcholine transport into synaptic vesicles. Electric lobe. Two classes of cysteines are involved in transport of acetylcholine. Binding of acetylcholine to VAChT involves one class of cysteine located at the cytoplasmic N-terminus and binding of vesamicol involves both. Organomercurial-mediated modification of two cysteine residues inhibits binding of vesamicol. Belongs to the major facilitator superfamily. Vesicular transporter family.