Hydrolyzes short- and long-chain N-acyl homoserine lactones with or without 3-oxo substitution at C3. Has slight activity towards L-homoserine lactone, and no activity towards gamma-butyrolactone. an N-acyl-L-homoserine lactone + H2O = an N-acyl-L-homoserine + H(+) Optimum pH is 8.0. Little or no activity below pH 4.2. Retains over 80% of maximum activity at 15 to 60 degrees Celsius. Activity is greatly reduced over 70 degrees Celsius.