Major isoform of alcohol oxidase, which catalyzes the oxidation of methanol to formaldehyde and hydrogen peroxide, the first step in the methanol utilization pathway of methylotrophic yeasts. a primary alcohol + O2 = an aldehyde + H2O2 Energy metabolism; methane degradation. Homooctamer. Induced by methanol. Subject to strong carbon catabolite repression. The C-terminal peroxisomal targeting signal (PTS) is essential for the efficient targeting and import of AOX into peroxisomes via the PTS1 pathway. Belongs to the GMC oxidoreductase family.