ATP + pyruvate = ADP + H(+) + phosphoenolpyruvate Not activated by classical allosteric effectors. Optimum pH is 6.0. Optimum temperature is higher than 98 degrees Celsius. Thermostable for 2 hours up to 100 degrees Celsius. Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5. Homotetramer. Belongs to the pyruvate kinase family.