Involved in the persistence and virulence of Mycobacterium. Catalyzes the reversible formation of succinate and glyoxylate from isocitrate, a key step of the glyoxylate cycle, which operates as an anaplerotic route for replenishing the tricarboxylic acid cycle during growth on fatty acid substrates. D-threo-isocitrate = glyoxylate + succinate Carbohydrate metabolism; glyoxylate cycle; (S)-malate from isocitrate: step 1/2. Homotetramer. cells lacking this gene show a slight residual isocitrate lyase activity. Belongs to the isocitrate lyase/PEP mutase superfamily. Isocitrate lyase family.