ATP + pyruvate = ADP + H(+) + phosphoenolpyruvate Regulated by phosphoenolpyruvate substrate and is allosterically activated by ribose-5-phosphate, AMP and other nucleoside monophosphates but not by fructose-1,6-bisphosphate. Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5. Homotetramer. Belongs to the pyruvate kinase family.