D-glyceraldehyde 3-phosphate + NAD(+) + phosphate = (2R)-3-phospho-glyceroyl phosphate + H(+) + NADH Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5. Homotetramer. On the 2D-gel the determined pI of this protein is: 7.0, its MW is: 37.5 kDa. Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.