Involved in extracellular matrix organization (PubMed:32333816). Required for the regulation of epidermal-basement membrane adhesion responsible for proper organogenesis during embryonic development (PubMed:12766769). Involved in brain organization and function (PubMed:32333816). Highly expressed in the apical ectodermal ridge of the limb buds from 10.5-12.5 dpc and expression was also detected in the interdigital spaces at 14.5 dpc. Found in cells just underlying the surface epithelium of the entire embryo and in the linings of the peritoneal cavity and dorsal aorta. At 12 dpc, detected in the mesonephric duct and in the lens (PubMed:12766769). Found in a linear fashion underlying the epidermis and the basal surface of other epithelia in embryos (PubMed:12766770). Found in meningeal and choroidal epidermal-basement membranes in embryos and neonates (PubMed:32333816). The Calx-beta domains bind calcium with high affinity and undergo a major conformational shift upon binding. Defects in Fras1 are the cause of blebbed (bl) phenotype, which is characterized by blister formation, syndactyly, eyelid fusion and renal agenesis. Subepidermal blisters are predominantly formed in the head region around the eyes and at the distal part of the limbs. As development proceeds blisters that are initially transparent gradually become hemorrhagic and embryos die between 14.5 dpc and 16.5 dpc. Homozygous adult knockout mice display impaired performance in various types of learning and memory tasks as well as reduced anxiety. Belongs to the FRAS1 family. Truncated C-terminus.