Effector proteins function to alter host cell physiology and promote bacterial survival in host tissues. This protein is an E3 ubiquitin ligase that interferes with host's ubiquitination pathway. Required for inducing polymorphonuclear leukocytes migration across the intestinal epithelium. Preferentially uses host UBE2D1 (UBCH5A), UBE2D2 (UBCH5B) and UBE2L3 (UBCH7) as E2 ubiquitin-conjugating enzymes. S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine. Interacts with SpaK/InvB. Secreted via type III secretion system 1 (SPI-1 TTSS), and delivered into the host cell. Can also be secreted through the flagellar export apparatus. The N-terminal 45 amino acids are necessary and sufficient for secretion and translocation into host cells. This N-terminal region binds the SpaK/InvB chaperone. Contains a C-terminal HECT-like domain. Ubiquitinated in the presence of host E1 ubiquitin-activating enzyme, E2 ubiquitin-conjugating enzyme and ubiquitin. Requires SpaK/InvB as a chaperone for its stability and for secretion. Belongs to the SopA E3 ligase family.