Phosphorylates proteins on serine, threonine, and tyrosine (By similarity). Probably associated with cell proliferation (By similarity). Phosphorylates MAD1L1 to promote mitotic checkpoint signaling (By similarity). Essential for chromosome alignment by enhancing AURKB activity (via direct CDCA8 phosphorylation) at the centromere, and for the mitotic checkpoint (By similarity). ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-[protein] ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-threonyl-[protein] ATP + L-tyrosyl-[protein] = ADP + H(+) + O-phospho-L-tyrosyl-[protein] Inhibited by the ATP-competitive kinase inhibitor, SP600125. Interacts with TPR; the interactions occurs in a microtubule-independent manner (By similarity). Interacts with MAD1L1 and MAD2L1 (By similarity). Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.