Splits dipeptides with a prolyl or hydroxyprolyl residue in the C-terminal position and a nonpolar amino acid at the N-terminal position. Also catalyzes the hydrolysis of toxic organophosphorus cholinesterase-inhibiting compounds including insecticide paraoxon and nerve gases such as diisopropylfluorophosphate (DFP), O-isopropyl methylphosphonofluoridate (sarin), O-pinacolyl methylphosphonofluoridate (soman), and O-cyclohexyl methylphosphonofluoridate. Hydrolysis of Xaa-|-Pro dipeptides. Also acts on aminoacyl-hydroxyproline analogs. No action on Pro-|-Pro. diisopropyl fluorophosphate + H2O = diisopropyl phosphate + fluoride + 2 H(+) An aryl dialkyl phosphate + H2O = dialkyl phosphate + an aryl alcohol. Binds 2 manganese ions per monomer. Optimum pH is 8.5 with DFP as substrate. Optimum temperature is 50 degrees Celsius. Monomer. Can be used for the catalytic detoxification of some nerve agents neurotoxins. Has a stereoselective preference for isomers with R-configuration at the phosphorous center of sarin and soman, and with S-configuration in phosphotriesters. Belongs to the peptidase M24B family. Bacterial-type prolidase subfamily.