This protein is a 'fusion' protein encoding four enzymatic activities of the pyrimidine pathway (GATase, CPSase, ATCase and DHOase). 2 ATP + H2O + hydrogencarbonate + L-glutamine = 2 ADP + carbamoyl phosphate + 2 H(+) + L-glutamate + phosphate carbamoyl phosphate + L-aspartate = H(+) + N-carbamoyl-L-aspartate + phosphate (S)-dihydroorotate + H2O = H(+) + N-carbamoyl-L-aspartate Binds 1 zinc ion per subunit (for dihydroorotase activity). Binds 4 magnesium or manganese ions per subunit. Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 1/3. Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 2/3. Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 3/3. GATase (glutamine amidotransferase) and CPSase (carbamoyl phosphate synthase) together form the glutamine-dependent CPSase (GD-CPSase) (EC 6.3.5.5). In the central section; belongs to the metallo-dependent hydrolases superfamily. DHOase family. CAD subfamily. Various problems, including DNA not present in the genome. Truncated N-terminus. Various problems, including DNA not present in the genome. Various problems, including DNA not present in the genome. Various problems, including DNA not present in the genome. Various problems, including DNA not present in the genome. Various problems, including DNA not present in the genome.