5' to 3' helicase that unwinds RNA and DNA duplexes in an ATP-dependent reaction (PubMed:19158098, PubMed:30218034, PubMed:22999958). Specific to 5'-phosphorylated single-stranded guanine-rich sequences (PubMed:8349627, PubMed:22999958). May play a role in RNA metabolism, ribosome biogenesis or initiation of translation (PubMed:19299493, PubMed:19158098). May play a role in regulation of transcription (By similarity). Interacts with tRNA-Tyr (PubMed:19299493). ATP + H2O = ADP + H(+) + phosphate ATP + H2O = ADP + H(+) + phosphate Homooligomer (PubMed:19299493). Interacts with RUVBL1 (PubMed:19299493). Interacts with RUVBL2 (PubMed:19299493). Interacts with GTF3C1 (PubMed:19299493). Interacts with ABT1 (PubMed:19299493). Interacts with ribosomes (PubMed:19158098). Expressed in all tissues examined. Expressed in the developing and adult human brain, with highest expression in the cerebellum. Moderately expressed in fibroblasts. The R3H domain recognizes phosphorylated 5'-ends of single-stranded nucleic acids which promotes binding of nucleic acids and stimulates ATPase activity. The disease is caused by variants affecting the gene represented in this entry. The disease is caused by variants affecting the gene represented in this entry. Belongs to the DNA2/NAM7 helicase family.