D-glyceraldehyde 3-phosphate + NAD(+) + phosphate = (2R)-3-phospho-glyceroyl phosphate + H(+) + NADH Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5. Homotetramer. There are three genes for G3PDH in yeast. Present with 169000 molecules/cell in log phase SD medium. Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.