Alpha-conotoxins act on postsynaptic membranes, they bind to the nicotinic acetylcholine receptors (nAChR) and thus inhibit them. Probable competitive antagonist of fish muscle acetylcholine receptor (PubMed:32245200). Inhibits postsynaptic nicotinic acetylcholine receptors (nAChRs) from zebrafish (IC(50)=58-100 nM) (PubMed:32245200). Blocks mammalian nAChR composed of alpha-1/gamma and alpha-1/delta subunits. Blocks central nervous system nAChR composed of alpha-4-beta-2/CHRNA4-CHRNB2 subunits and peripheral nervous system nAChR composed of alpha-3-beta-4/CHRNA3-CHRNB4 subunits. Low toxin concentrations potentiate currents in muscle nAChR composed of alpha-1-beta-1-gamma-delta (CHRNA1-CHRNB1-CHRNG-CHRND) subunits and central nervous system nAChR composed of alpha-4-beta-2/CHRNA4-CHRNB2 subunits, but not peripheral nervous system nAChR composed of alpha-3-beta-4 subunits. Also exhibits inhibition of D.melanogaster alpha-7/CHRNA7 nAChRs (PubMed:25466886). Has possibly a distinct nAChR binding mode from other alpha-conotoxins, due to a different three residue motif (lacks the Ser-Xaa-Pro motif) (By similarity). Expressed by the venom duct. The cysteine framework is I (CC-C-C). Alpha4/7 pattern. Belongs to the conotoxin A superfamily.