This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. Monomer. Expressed in skeletal muscle, heart, brain and aorta. Not expressed in liver, kidney, spleen and lung. Methylated (PubMed:9518480). Trimethylated at Lys-165 by EEF1AKMT3. Mono-, di-, and trimethylated at Lys-36 by EEF1AKMT4; trimethylated form is predominant. Methylation by EEF1AKMT4 contributes to the fine-tuning of translation rates for a subset of tRNAs (By similarity). Trimethylated at the N-terminus and dimethylated at Lys-55 by METTL13 (By similarity). Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.