Gating-modifier toxin that inhibits both sodium (Nav) and calcium (Cav3) channels by inducing hyperpolarizing shift in voltage-dependence of activation and steady state inactivation. Inhibits Nav1.1/SCN1A, Nav1.2/SCN2A, Nav1.3/SCN3A, Nav1.6/SCN6A, Nav1.7/SCN9A and Cav3.1/CACNA1G sodium and calcium channels at nanomolar concentrations (IC(50)=81-301 nM). Surprisingly, selectively slows fast inactivation of Nav1.3/SCN3A. Also shows moderate inhibition of Cav3.2/CACNA1H calcium channels (IC(50)=1233 nM). Ex vivo, nearly ablates neuronal mechanosensitivity in afferent fibers innervating the colon and the bladder. In vivo, in a mouse model of irritable bowel syndrome, intracolonic administration of the toxin reverses colonic mechanical hypersensitivity. Expressed by the venom gland. The presence of a 'disulfide through disulfide knot' structurally defines this protein as a knottin. Monoisotopic mass. Could be a potential drug lead to treat chronic visceral pain. Shows no or weak activity on Nav1.4/SCN4A, Nav1.5/SCN5A, and Cav3.3/CACNA1I sodium and calcium channels. Belongs to the neurotoxin 10 (Hwtx-1) family. 59 (Tltx) subfamily.