Myozenins may serve as intracellular binding proteins involved in linking Z line proteins such as alpha-actinin, gamma-filamin, TCAP/telethonin, LDB3/ZASP and localizing calcineurin signaling to the sarcomere. Plays an important role in the modulation of calcineurin signaling. May play a role in myofibrillogenesis. Interacts via its C-terminus with spectrin repeats 3 and 4 of ACTN2. Interacts with ACTN1, LDB3, MYOT and PPP3CA (By similarity). Colocalizes with ACTN1 and PPP3CA at the Z-line of heart and skeletal muscle. Expressed specifically in heart and skeletal muscle. In skeletal muscle, localized to the soleus and plantaris muscles, which are predominantly composed of slow-twitch fibers. At 9.5 dpc, expressed weakly in heart. Higher levels of expression detected at 12.5 dpc and 15.5 dpc in both cardiac and skeletal muscle. Belongs to the myozenin family.