Metalloprotease with anticoagulant activity. Cleaves fibrinogen Aalpha (FGA), gamma (FGG) and Bbeta (FGB) chains after positions 'Asn-121', 'Lys-160' and 'Pro-102', respectively. Breaks down cross-linked and non-cross-linked fibrin clots. Prevents and reverts platelet aggregation induced by ADP and collagen. Prevents thrombin-induced platelet clotting. Does not affect plasma levels of coagulation factors prothrombin (F2), V (F5), VII (F7), VIII (F8), IX (F9), X (F10), XI (F11), XII (F12), plasma kallikrein (KLKB1) and kininogen-1 (KNG1). Inhibited by EDTA, cysteine, DTT and sodium phosphate. Partially inhibited by EGTA, citrate, Tris and glycine. Not inhibited by DFP, PMSF, iodacetic acid and leupeptin. Requires sodium chloride concentrations higher than 0.15 M for activity. Optimum pH is 7.5. Active from pH 5.5 to 11. Active at 37 degrees Celsius. Activity reversibly reduced to 26% and 0% at 25 degrees Celsius and 4 degrees Celsius, respectively. Complete and irreversible loss of activity after 15 min at 60 degrees Celsius. Expressed mainly in the posterior salivary glands and, to a lesser extent, in the anterior salivary glands and secreted into the proboscis (at protein level). Presumably not injected into the host's bloodstream, as feeding by H.ghilianii does not cause prolonged bleeding of resulting wounds.