Acts in cell cycle and stress checkpoint signaling by sequestering signal transducers regulated by the checkpoints (PubMed:11818066, PubMed:8036497, PubMed:15629716). Required for the DNA damage checkpoint that ensures that DNA damage is repaired before mitosis is attempted (PubMed:8036497). During environmental stress, sequesters srk1-phosphorylated cdc25 in the cytoplasm to delay the G2/M transition (PubMed:15629716). Sequesters byr2 in the cytoplasm to prevent its translocation to the plasma membrane (PubMed:12242289). Sequesters ran1/pat1-phosphorylated mei2 from its non-coding RNA activators (including meiRNA), to prevent meiotic induction in vegetative cells and to regulate meiosis I (PubMed:11818066). Homodimer (PubMed:12242289). Binds preferentially to mei2 phosphorylated by ran1/pat1 (PubMed:11818066). Binds preferentially to cdc25 phosphorylated by srk1 during G2; the interaction is increased during osmotic stress (PubMed:15629716). Interacts with byr2 (PubMed:12242289). Interacts with rad25 (PubMed:12242289). Belongs to the 14-3-3 family.