Transcription factor that plays a key role in neuronal differentiation (PubMed:9373037). Acts by specifically repressing expression of non-neuronal genes during neuron differentiation (By similarity). In contrast to other transcription repressors that inhibit specific lineages, mediates repression of multiple differentiation programs (By similarity). Also represses expression of negative regulators of neurogenesis, such as members of the Notch signaling pathway, including HES1 (By similarity). The combination of three transcription factors, ASCL1, POU3F2/BRN2 and MYT1L, is sufficient to reprogram fibroblasts and other somatic cells into induced neuronal (iN) cells in vitro (By similarity). Directly binds the 5'-AAGTT-3' core motif present on the promoter of target genes and represses transcription by recruiting a multiprotein complex containing SIN3B (By similarity). The 5'-AAGTT-3' core motif is absent from the promoter of neural genes (By similarity). Interacts with SIN3B. Preferentially binds to DNA binding sites that are in an open chromatin configuration. Brain, testis and pituitary gland. Expression is higher in the brain than in the testis and pituitary gland. Highest level expression seen in the developing CNS. Detected at E11.5 and expression is seen throughout the proliferating cortex neuroepithelium, developing medulla, and spinal cord. At E12.5 found in the nasal epithelium and at E13.5 detected in the trigeminal ganglia, dorsal root ganglia and the ganglion cell layer of the retina. At E14-E15 expressed at high levels in the brain and spinal cord and then levels subsequently decrease. Belongs to the MYT1 family.