E3 ubiquitin ligase that plays a crucial role in the activation of the IKBKE-dependent branch of the type I interferon signaling pathway (PubMed:24882218, PubMed:31694946). In concert with the ubiquitin-conjugating E2 enzyme UBE2K, synthesizes unanchored 'Lys-48'-linked polyubiquitin chains that promote the oligomerization and autophosphorylation of IKBKE leading to stimulation of an antiviral response (PubMed:24882218). Ubiquitinates also MYC and inhibits its transcription activation activity, maintaining the pluripotency of embryonic stem cells (By similarity). S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine. Protein modification; protein ubiquitination. Homotrimer (PubMed:17156811). Forms heteromultimers (via B30.2/SPRY domain) with TRIM5 (PubMed:21680743). Interacts with MYC (By similarity). Interacts (via SPRY domain) with IKBKE (PubMed:24882218). Interacts with VAMP8; this interaction contributes to the activation of the type I interferon antiviral response (PubMed:31694946). (Microbial infection) Interacts with ebolavirus protein VP35; this interaction plays an important role in promoting efficient viral replication. (Microbial infection) Interacts with HIV-1 capsid complexes. (Microbial infection) Interacts with matrix protein of Nipah virus; this interaction inhibits the IKBKE-dependent activation of the type I interferon signaling pathway. The B-box zinc finger and the linker region between the coiled coil and B30.2/SPRY domains contribute to higher order self-association. Belongs to the TRIM/RBCC family. Chimeric cDNA. The in vivo relevance of this transcript of the TRIM34 (AC Q9BYJ4) and TRIM6 genes that codes for a chimeric protein of 842 residues is uncertain.