Alpha-conotoxins act on postsynaptic membranes, they bind to the nicotinic acetylcholine receptors (nAChR) and thus inhibit them (By similarity). This toxin also inhibits high voltage-activated (HVA) calcium channel currents in rat DRG neurons (27% inhibition at 1 uM toxin) probably by activating GABA(B) receptors (GABBR1 and/or GABBR2) (PubMed:26948522). Expressed by the venom duct. The cysteine framework is I (CC-C-C). Alpha4/6 pattern. Non-native isomers 'ribbon' (with disulfide connectivity C1-C4, C2-C3) and 'beads' (with disulfide connectivity C1-C2, C3-C4) also inhibit high voltage-activated (HVA) calcium channel currents in rat DRG neurons (25-30% inhibition at 1 uM toxin) (PubMed:26948522). Mutants Pu1.2(9-16), [C3S; C9S]Pu1.2 and [C4S]Pu1.2(1-9) are all C-terminally amidated. This toxin does not inhibit human alpha-7/CHRNA7 and alpha-9-alpha-10/CHRNA9-CHRNA10 acetylcholine receptors (when tested at 3 uM) (PubMed:26948522). Belongs to the conotoxin A superfamily.