Involved in peroxisomal fatty acid beta-oxidation. Required for wound-induced jasmonate biosynthesis. Possesses enoyl-CoA hydratase activity against short chain substrates (C4-C6) and 3-hydroxyacyl-CoA dehydrogenase activity against chains of variable sizes (C6-C16) (PubMed:10521521, PubMed:17544464, PubMed:20463021). Possesses cinnamoyl-CoA hydratase activity and is involved in the peroxisomal beta-oxidation pathway for the biosynthesis of benzoic acid (BA). Required for the accumulation in seeds of benzoylated glucosinolates (BGs) and substituted hydroxybenzoylated choline esters, which are BA-containing secondary metabolites. Required for salicylic acid (SA) in seeds (PubMed:24254312). a (3S)-3-hydroxyacyl-CoA = a (2E)-enoyl-CoA + H2O a 4-saturated-(3S)-3-hydroxyacyl-CoA = a (3E)-enoyl-CoA + H2O (3S)-3-hydroxybutanoyl-CoA = (2E)-butenoyl-CoA + H2O (3S)-hydroxyoctanoyl-CoA = (2E)-octenoyl-CoA + H2O (3S)-3-hydroxydodecanoyl-CoA = (2E)-dodecenoyl-CoA + H2O (3S)-hydroxytetradecanoyl-CoA = (2E)-tetradecenoyl-CoA + H2O (3S)-hydroxyhexanoyl-CoA = (2E)-hexenoyl-CoA + H2O a (3Z)-enoyl-CoA = a 4-saturated (2E)-enoyl-CoA a (3E)-enoyl-CoA = a 4-saturated (2E)-enoyl-CoA (3S)-3-hydroxybutanoyl-CoA = (3R)-3-hydroxybutanoyl-CoA a (3S)-3-hydroxyacyl-CoA + NAD(+) = a 3-oxoacyl-CoA + H(+) + NADH (3S)-3-hydroxybutanoyl-CoA + NAD(+) = acetoacetyl-CoA + H(+) + NADH (3S)-hydroxyhexanoyl-CoA + NAD(+) = 3-oxohexanoyl-CoA + H(+) + NADH (3S)-hydroxyoctanoyl-CoA + NAD(+) = 3-oxooctanoyl-CoA + H(+) + NADH (3S)-3-hydroxydodecanoyl-CoA + NAD(+) = 3-oxododecanoyl-CoA + H(+) + NADH (3S)-hydroxytetradecanoyl-CoA + NAD(+) = 3-oxotetradecanoyl-CoA + H(+) + NADH Lipid metabolism; fatty acid beta-oxidation. Widely expressed. The epimerase and isomerase activities are contained in the N-terminal region while the dehydrogenase activity is in the C-terminal region. Reduced rosette size, twisted leaves, and abnormal and sterile flowers. In the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family. In the central section; belongs to the 3-hydroxyacyl-CoA dehydrogenase family.