Required for vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seems to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin (By similarity). Involved in spikelet development (PubMed:25848690). Regulates protein storage vacuole (PSV) morphology in endosperm cells (PubMed:25848690). Forms a quaternary complex with the two glutelin zipcode RNA-binding proteins RBP-L and RBP-P, and the membrane trafficking factor RAB5A (PubMed:32471860). This quaternay complex carries glutelin mRNAs for active transport on endosomes to the cortical endoplasmic reticulum membrane, and enables endosome-mediated glutelin mRNA transport in endosperm cells (PubMed:32471860). ATP + H2O = ADP + H(+) + phosphate Binds 1 Mg(2+) ion per subunit. Homohexamer (By similarity). Binds to SNARE-SNAP complexes to form 20S particles (Probable). Interacts with the 60S acidic ribosomal protein P0 (60SP0) (PubMed:25848690). Forms a complex with the heterologous pentameric complex formed by P0 and dimers of P1 and P2 (PubMed:25848690). Interacts with RBP-P and RAB5A (PubMed:32471860). Highly expressed in stems, inflorescences and immature seeds (at protein level) (PubMed:25848690). Expressed in leaves and mature seeds (at protein level) (PubMed:25848690). Overexpression of NSF decreases plant height and grain weight, and alters the morphology of protein bodies. Belongs to the AAA ATPase family.