Phosphorylates proteins on serine, threonine, and tyrosine (PubMed:18243099, PubMed:29162720). Probably associated with cell proliferation (PubMed:18243099). Phosphorylates MAD1L1 to promote mitotic checkpoint signaling (PubMed:29162720). Essential for chromosome alignment by enhancing AURKB activity (via direct CDCA8 phosphorylation) at the centromere, and for the mitotic checkpoint (PubMed:18243099). ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-[protein] ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-threonyl-[protein] ATP + L-tyrosyl-[protein] = ADP + H(+) + O-phospho-L-tyrosyl-[protein] Inhibited by the ATP-competitive kinase inhibitor, SP600125. Interacts with TPR; the interactions occurs in a microtubule-independent manner (PubMed:19273613). Interacts with MAD1L1 and MAD2L1 (PubMed:29162720). Present in rapidly proliferating cell lines. Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.