Involved in nucleotide metabolism: produces dUMP, the immediate precursor of thymidine nucleotides and decreases the intracellular concentration of dUTP, so that uracil cannot be incorporated into DNA (Probable). The Ser-64 side chain changes its position upon ligand-binding to make contacts with the nucleotide phosphates (PubMed:20823546). dUTP + H2O = diphosphate + dUMP + H(+) Binds 1 Mg(2+) per subunit, coordinated entirely by the nucleotide and ordered water molecules. Pyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP (dUTP route): step 2/2. Homotrimer. The uracil deoxyribose moiety interacts with the protein through Ile-81 and Tyr-85, which discriminate against the ribose form of the nucleotide. No visible phenotype; double yncF-yosS deletions (both encode dUTPases) are also viable. Belongs to the dUTPase family.