Cytochrome P450 monooxygenase involved in the biosynthesis of ergosterol (By similarity). The existence of several duplicated sterol 14-alpha demethylase genes could be a good strategy to modulate the composition and fluidity of the cell membrane (By similarity). Catalyzes C14-demethylation of erburicol to produce 4,4,24-trimethyl cholesta-8,24(28)-dien-3-beta-ol (By similarity). Shows a slight substrate preference toward eburicol over obtusifoliol (By similarity). As a target of azole drugs, plays a crucial role in azole susceptibility (PubMed:18775650, PubMed:29311090). a 14alpha-methyl steroid + 3 O2 + 3 reduced [NADPH--hemoprotein reductase] = a Delta(14) steroid + formate + 4 H(+) + 4 H2O + 3 oxidized [NADPH--hemoprotein reductase] Steroid biosynthesis; sterol biosynthesis. Over-expressed in azole-resistant clinical isolates. Belongs to the cytochrome P450 family.