NADPH-dependent oxidoreductase which catalyzes the reduction of a variety of compounds bearing carbonyl groups including ketosteroids, alpha-dicarbonyl compounds, aldehydes, aromatic ketones and quinones (PubMed:12604222, PubMed:19056333). Reduces all-trans-retinal and 9-cis retinal (Probable). Reduces 3-ketosteroids and benzil into 3alpha-hydroxysteroids and S-benzoin, respectively, in contrast to the stereoselectivity of primates DHRS4s which produce 3beta-hydroxysteroids and R-benzoin (PubMed:19056333). In the reverse reaction, catalyzes the NADP-dependent oxidation of 3alpha-hydroxysteroids and alcohol, but with much lower efficiency (PubMed:19056333). Involved in the metabolism of 3alpha-hydroxysteroids, retinoid, isatin and xenobiotic carbonyl compounds (PubMed:12604222, PubMed:19056333). a secondary alcohol + NADP(+) = a ketone + H(+) + NADPH 3alpha-hydroxy-5beta-pregnan-20-one + NADP(+) = 5beta-pregnan-3,20-dione + H(+) + NADPH 5beta-dihydrotestosterone + H(+) + NADPH = 5beta-androstane-3alpha,17beta-diol + NADP(+) all-trans-retinol + NADP(+) = all-trans-retinal + H(+) + NADPH H(+) + isatin + NADPH = 3-hydroxyindolin-2-one + NADP(+) Inhibited by kaempferol, quercetin, genistein and myristic acid. kcat is 45 min(-1) with all-trans-Retinal as substrate (at pH7.4) (PubMed:12604222). kcat is 990 min(-1) with isatin as substrate (at pH6) (PubMed:12604222). kcat is 1180 min(-1) with 1-phenyl-1,2-propanedione as substrate (at pH6) (PubMed:12604222). kcat is 780 min(-1) with Diacetyl as substrate (at pH6) (PubMed:12604222). kcat is 650 min(-1) with Pyridine-4-aldehyde as substrate (at pH6) (PubMed:12604222). kcat is 1.5 min(-1) with 5beta-Pregnane-3,20-dione as substrate (at pH7.4) (PubMed:19056333). kcat is 3.7 min(-1) with 5beta-Pregnane-3,20-dione as substrate (at pH7.4) (PubMed:19056333). kcat is 4.6 min(-1) with 5beta-Androstan-17beta-ol-3-one as substrate (at pH7.4) (PubMed:19056333). kcat is 16 min(-1) with Dehydrolithocholic acid as substrate (at pH7.4) (PubMed:19056333). kcat is 814 min(-1) with Dimethyl-2-oxoglutarate as substrate (at pH6) (PubMed:19056333). kcat is 920 min(-1) with Benzil as substrate (at pH6) (PubMed:19056333). kcat is 650 min(-1) with 2,3-Pentanedione as substrate (at pH6) (PubMed:19056333). kcat is 1200 min(-1) with Methyl benzoylformate as substrate (at pH6) (PubMed:19056333). kcat is 890 min(-1) with 4-Nitrobenzaldehyde as substrate (at pH6) (PubMed:19056333). kcat is 0.02 min(-1) with 5beta-Pregnan-3alpha-ol-20-one as substrate (at pH6) (PubMed:19056333). kcat is 0.04 min(-1) with 5beta-Androstane-3alpha,17beta-diol as substrate (at pH6) (PubMed:19056333). kcat is 0.06 min(-1) with Lithocholic acid as substrate (at pH6) (PubMed:19056333). Homotetramer. Detected in heart, kidney, liver and small intestine. Detected at lower levels in brain, lung, stomach and spleen. The C-terminus peroxisomal targeting signal tripeptide is important for peroxisomal import. Once in the peroxisome, it is involved in intersubunit interactions. Three specific residues, Phe-177, Leu-180 and Asn-196 are conserved between non-primate mammals whereas the respective residues are serine, phenylalanine and threonine in primates (PubMed:19056333). The two residues at positions 177 and 180 are molecular determinants responsible for the stereoselective reduction of 3-ketosteroids and benzil (PubMed:19056333). The presence of an asparagine at position 196 is important for the maintenance of the quaternary structure resulting in stability at cold temperature and improved catalytic activity toward retinal (PubMed:19056333). Primate DHRS4s display different stereoselectivity and catalytic efficiency in the oxidoreduction of some substrates as compared to other mammal DHRS4s due to a difference in conserved amino acid residues. Belongs to the short-chain dehydrogenases/reductases (SDR) family. Truncated N-terminus.