When overexpressed the N-terminus (residues 1-476) cleaves rat synaptobrevin-2/VAMP2 between '89-Trp-|-Trp-90' in vitro. This releases the cytoplasmic domain of VAMP2 from the synaptic vesicle membrane, which would prevent the assembly of the trans-SNARE complex on the membrane and thus prevent vesicle-target membrane fusion and neurotransmitter release (PubMed:27443638). Limited hydrolysis of proteins of the neuroexocytosis apparatus, synaptobrevins, SNAP25 or syntaxin. No detected action on small molecule substrates. Inhibited by EDTA and 1,10-phenanthroline (PubMed:27443638). There are seven antigenically distinct forms of botulinum neurotoxin: Types A, B, C, D, E, F, and G, but serologically this does not belong to any of them. Belongs to the peptidase M27 family. This bacteria is missing genes associated with the botulinum neurotoxin cluster in Clostridia (botR, hemagglutinin genes and ORF-X proteins), so it is not clear if it is actually a toxin. The in situ target might not be animal as this bacteria was isolated from fermented rice grains.