Involved in resistance to gentamicin, tobramycin, and kanamycin. Tobramycin and kanamycin resistance is due to the ACC activity, specified by N-terminal region. The C-terminal region is a kinase that phosphorylates several 4,6-disubstituted aminoglycosides. a gentamycin + GTP = a gentamycin 2''-phosphate + GDP + H(+) In the C-terminal section; belongs to the aminoglycoside phosphotransferase family.