Lethal toxin with multiple biological activities. Inhibits voltage-gated TTX-sensitive sodium channels in DRG neurons (IC(50)=55 nM) and also shows activity when directly tested on Nav1.7/SCN9A (IC(50)=25.1-630 nM) (PubMed:17451655, PubMed:21731778, PubMed:31234412). Inhibits N-type calcium channels (Cav2.2/CACNA1B (IC(50)=100 nM)) (PubMed:11024489, PubMed:17451655). Also blocks neuromuscular transmission (PubMed:8212049, PubMed:9028007, PubMed:10736477). In vivo, intrathecal injected toxin shows analgesic activity in the rat formalin-induced pain model, without induction of motor dysfunction in rats. Expressed by the venom gland. The presence of a 'disulfide through disulfide knot' structurally defines this protein as a knottin. LD(50) is 0.7 mg/kg by intraperitoneal injection into mice. LD(50) is 9.40 ug/kg by intracisternal injection into mice. Shows very weak effect on calcium channel Cav1 subtypes (PubMed:11024489, PubMed:17451655). Does not show effect on calcium channel Cav3 subtypes (PubMed:11024489, PubMed:17451655). Does not show effect on voltage-gated potassium channels Kv (delayed rectifier) (PubMed:11024489). Does not show effect on voltage-gated TTX-resistant sodium channels (PubMed:17451655). Is the most abundant toxin in the crude venom. Belongs to the neurotoxin 10 (Hwtx-1) family. 23 (HwTx-I) subfamily.