Binds to single-stranded sequences participating in DNA replication in addition to those mediating transcriptional repression (URS1) and activation (CAR1). Stimulates the activity of a cognate strand exchange protein (SEP1). It cooperates with T-AG and DNA topoisomerase I to unwind template DNA containing the simian virus 40 origin of DNA replication. Heterotrimer of 69, 36, and 13 kDa chains. The DNA-binding activity may reside exclusively on the 69 kDa subunit. Interacts with MCM10. Phosphorylated in a cell cycle-dependent manner with phosphorylation increasing at the entry in S phase and dephosphorylation occurring at mitosis. The N-terminus is blocked. Present with 6080 molecules/cell in log phase SD medium. Belongs to the replication factor A protein 2 family.