Oxidizes aliphatic acyl-CoA substrates of different chain lengths such as hexanoyl-CoA, decanoyl-CoA and myristoyl-CoA as well as aromatic/heterocyclic ring-substituted chromogenic substrates, such as furylpropionyl-CoA. Of the above substrates, the efficiency of the enzyme, exhibits the following order: decanoyl-CoA > myristoyl-CoA > hexanoyl-CoA > furyl-propionyl-CoA. a 2,3-saturated acyl-CoA + O2 = a (2E)-enoyl-CoA + H2O2 Optimum pH is 7.4. Optimum temperature is 23-38 degrees Celsius. Lipid metabolism; peroxisomal fatty acid beta-oxidation. Heteropentamer composed of five different subunits. Belongs to the acyl-CoA oxidase family.