A cytochrome P450 monooxygenase involved in the metabolism of fatty acids. Catalyzes predominantly the oxidation of the terminal carbon (omega-oxidation) of saturated and unsaturated fatty acids (PubMed:10620324, PubMed:10869363, PubMed:15618658). May act as a major omega-hydroxylase for dodecanoic (lauric) acid in kidney (PubMed:10620324, PubMed:10869363, PubMed:15618658). At preglomerular arteries, may participate in omega-hydroxylation of (5Z,8Z,11Z,14Z)-eicosatetraenoic acid (arachidonate) to 20-hydroxyeicosatetraenoic acid (20-HETE), a signaling molecule acting both as vasoconstrictive and natriuretic with overall effect on arterial blood pressure (PubMed:15618658). Can also catalyze the oxidation of the penultimate carbon (omega-1 oxidation) of fatty acids with lower efficiency, displaying a preference for the (R)-stereoisomer (PubMed:10869363). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase) (PubMed:10620324, PubMed:10869363, PubMed:15618658). an organic molecule + O2 + reduced [NADPH--hemoprotein reductase] = an alcohol + H(+) + H2O + oxidized [NADPH--hemoprotein reductase] dodecanoate + O2 + reduced [NADPH--hemoprotein reductase] = 12-hydroxydodecanoate + H(+) + H2O + oxidized [NADPH--hemoprotein reductase] dodecanoate + O2 + reduced [NADPH--hemoprotein reductase] = (11R)-hydroxydodecanoate + H(+) + H2O + oxidized [NADPH--hemoprotein reductase] (5Z,8Z,11Z,14Z)-eicosatetraenoate + O2 + reduced [NADPH--hemoprotein reductase] = 20-hydroxy-(5Z,8Z,11Z,14Z)-eicosatetraenoate + H(+) + H2O + oxidized [NADPH--hemoprotein reductase] O2 + prostaglandin A1 + reduced [NADPH--hemoprotein reductase] = 20-hydroxy prostaglandin A1 + H(+) + H2O + oxidized [NADPH--hemoprotein reductase] Activated by cytochrome b5 and phosphatidylserine. Lipid metabolism; fatty acid metabolism. Expressed at proximal straight tubules and preglomerular arteries of the outer medulla as well in the cortex regions of kidney (at protein level). Belongs to the cytochrome P450 family.