Catalyzes the desaturation of acyl-CoAs to 2-trans-enoyl-CoAs. First enzyme of the fatty acid beta-oxidation pathway. a 2,3-saturated acyl-CoA + O2 = a (2E)-enoyl-CoA + H2O2 Lipid metabolism; peroxisomal fatty acid beta-oxidation. Homodimer. Expressed in glia. Expressed in glia. Most mutants die as pupae. They have increased VLCFA, loss of vision and have glial loss and reduced neuronal survival. Belongs to the acyl-CoA oxidase family.