Required for formation of the 2-thio group of the 5-methoxycarbonylmethyl-2-thiouridine modified base in some tRNAs. hydrogen cyanide + thiosulfate = 2 H(+) + sulfite + thiocyanate Contains two rhodanese domains with different primary structures but with near identical secondary structure conformations suggesting a common evolutionary origin. Only the C-terminal rhodanese domain contains the catalytic cysteine residue (By similarity). Present with 5770 molecules/cell in log phase SD medium.