Bile salt hydrolase that catalyzes the deconjugation of glycine- and taurine-linked bile salts, which occurs naturally in the intestines of animals, releasing amino acid residues and deconjugated bile salts (bile acids). Can hydrolyze the amid bond in the bile salts glycocholate (GCA), glycodeoxycholate (GDCA), glycochenodeoxycholate (GCDCA), taurocholate (TCA), taurodeoxycholate (TDCA) and taurochenodeoxycholate (TCDCA). Shows a preference for glycine-conjugated bile acids as substrates. glycocholate + H2O = cholate + glycine glycodeoxycholate + H2O = deoxycholate + glycine chenodeoxycholate + glycine = glycochenodeoxycholate + H2O cholate + taurine = H2O + taurocholate H2O + taurodeoxycholate = deoxycholate + taurine H2O + taurochenodeoxycholate = chenodeoxycholate + taurine Optimum pH is 4.7-5.5. Optimum temperature is 30-45 degrees Celsius. Lipid metabolism; bile acid biosynthesis. Homotetramer. The tetramer consists of a dimer of dimers. Belongs to the peptidase C59 family.