Dermonecrotic toxins cleave the phosphodiester linkage between the phosphate and headgroup of certain phospholipids (sphingolipid and lysolipid substrates), forming an alcohol (often choline) and a cyclic phosphate (PubMed:24009677, PubMed:25752604). This toxin acts on sphingomyelin (SM) with high activity and on lysophosphatidylcholine (LPC) and ceramide phosphoethanolamine (CPE) with low activity (PubMed:24009677, PubMed:25752604). In vivo, shows potent insecticidal activities (PubMed:22561243). On mammals, induces dermonecrosis, hemolysis, increased vascular permeability, edema, inflammatory response, and platelet aggregation (By similarity). an N-(acyl)-sphingosylphosphocholine = an N-(acyl)-sphingosyl-1,3-cyclic phosphate + choline N-hexanoyl-sphing-4-enine-1-phosphocholine = choline + N-(hexanoyl)-sphing-4-enine-1,3-cyclic phosphate N-(dodecanoyl)-sphing-4-enine-1-phosphocholine = choline + N-dodecanoyl-sphing-4-enine-1,3-cyclic phosphate a 1-acyl-sn-glycero-3-phosphocholine = a 1-acyl-sn-glycero-2,3-cyclic phosphate + choline 1-tetradecanoyl-sn-glycero-3-phosphocholine = 1-tetradecanoyl-sn-glycero-2,3-cyclic phosphate + choline 1-octanoyl-sn-glycero-3-phosphocholine = 1-octanoyl-sn-glycero-2,3-cyclic phosphate + choline 1-hexadecanoyl-sn-glycero-3-phosphocholine = 1-hexadecanoyl-sn-glycero-2,3-cyclic phosphate + choline an N-(acyl)-sphingosylphosphoethanolamine = an N-(acyl)-sphingosyl-1,3-cyclic phosphate + ethanolamine N-dodecanoyl-heptadecasphing-4-enine-1-phosphoethanolamine = ethanolamine + N-dodecanoyl-heptadecasphing-4-enine-1,3-cyclic phosphate Binds 1 Mg(2+) ion per subunit. Expressed by the venom gland. PD(50) is 2.27 +-0.43 ug/g when injected into cricket (Acheta domestica) (observed at 60 minutes). LD(50) is 2.27 +-0.43 ug/g when injected into cricket (Acheta domestica) (observed at 24 hours). Belongs to the arthropod phospholipase D family. Class II subfamily. The most common activity assay for dermonecrotic toxins detects catalytic activity by monitoring choline release from substrate. Liberation of choline from sphingomyelin (SM) or lysophosphatidylcholine (LPC) is commonly assumed to result from substrate hydrolysis, giving either ceramide-1-phosphate (C1P) or lysophosphatidic acid (LPA), respectively, as a second product. However, two studies from Lajoie and colleagues (2013 and 2015) report the observation of exclusive formation of cyclic phosphate products as second products, resulting from intramolecular transphosphatidylation. Cyclic phosphates have vastly different biological properties from their monoester counterparts, and they may be relevant to the pathology of brown spider envenomation.