Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. ATP + pyruvate = ADP + H(+) + phosphoenolpyruvate Allosterically activated by fructose 1,6-bisphosphate. Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5. Homotetramer. This activity is regulated by glucose levels. Belongs to the pyruvate kinase family.