Shows trans-enoyl-CoA hydratase/3-hydroxyacyl-CoA dehydratase activity (PubMed:17240207). In vitro, can hydrate various enoyl-CoA such as (2E)-hexenoyl-CoA, (2E)-octenoyl-CoA, (2E)-decenoyl-CoA, (2E)-dodecenoyl-CoA and (2E)-hexadecenoyl-CoA (PubMed:17240207, PubMed:19136596). May contribute to the persistence of the tuberculosis infection by inducing COX-2 expression in macrophages through MAPK-NF-kappaB signaling pathway (PubMed:24907510). a (3R)-3-hydroxyacyl-CoA = a (2E)-enoyl-CoA + H2O (3R)-hydroxyhexanoyl-CoA = (2E)-hexenoyl-CoA + H2O (2E)-octenoyl-CoA + H2O = (3R)-hydroxyoctanoyl-CoA (3R)-3-hydroxydecanoyl-CoA = (2E)-decenoyl-CoA + H2O (3R)-3-hydroxydodecanoyl-CoA = (2E)-dodecenoyl-CoA + H2O (3R)-hydroxyhexadecanoyl-CoA = (2E)-hexadecenoyl-CoA + H2O kcat is 45 sec(-1) with dodecenoyl-CoA as substrate. Belongs to the enoyl-CoA hydratase/isomerase family.