After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. The channel is blocked by alpha-bungarotoxin. Homopentamer. Homooligomer of the short form gives rise to unfunctional channels, as does coexpression of both long and short forms of the receptor (PubMed:7620615). Interacts with RIC3; which is required for proper folding and assembly. Interacts with LYPD6 (By similarity). Interacts with the alpha-conotoxin RgIA (By similarity). Interacts with alpha-conotoxins ImI and ImII (By similarity). Interacts with CANX (By similarity). TMEM35A/NACHO and RIC3 promote its trafficking to the cell membrane. At least in chromaffin cells. Glycosylations at Asn-43, Asn-87 and Asn-130 are essential for TMEM35A/NACHO-mediated proper subunit assembly and trafficking to the cell membrane. Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Alpha-7/CHRNA7 sub-subfamily.