Specific citrate synthase with catalytic activity only with acetyl-CoA. acetyl-CoA + H2O + oxaloacetate = citrate + CoA + H(+) Phosphorylation at Ser-462 inhibits catalytic activity. Dephosphorylation at Ser-462 by PTC7 enhances catalytic activity. Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate from oxaloacetate: step 1/2. Monomer and homodimer (PubMed:28076776). Exists as an inactive monomer when phosphorylated (PubMed:28076776). Homodimerization is dependent on dephosphorylation of Ser-462 by PTC7 and is required for activity (PubMed:28076776). Phosphorylation at Ser-462. Dephosphorylated at Ser-462 by PTC7. Decreased growth related to mitochondrial dysfunction. Citrate synthase is found in nearly all cells capable of oxidative metabolism. Belongs to the citrate synthase family.