DNA repair enzyme that can remove a variety of covalent adducts from DNA through hydrolysis of a 3'-phosphodiester bond, giving rise to DNA with a free 3' phosphate. Catalyzes the hydrolysis of dead-end complexes between DNA and the topoisomerase I active site tyrosine residue. Inhibited by vanadate analogs. kcat is 1077 min(-1) with 18-mer single-stranded oligo with a 3'-phosphotyrosine as substrate. Ubiquitous, with a low level in roots. The TDP domain (123-605) is sufficient to confer the full phosphodiesterase activity. Developmental defects, including loss of apical dominance, early flowering and dwarf phenotype, when homozygous (PubMed:20876339). Hypersensitivity to camptothecin and failure of DNA damage repair resulting in progressive cell death (PubMed:20876339). No visible phenotype when heterozygous (PubMed:20876339). Belongs to the tyrosyl-DNA phosphodiesterase family.