Non specific carboxylic ester hydrolase. Hydrolyzes various pNP-esters, with a preference for short carbon chain substrates. Can also hydrolyze tributyrin to di- and monobutyrin and 4-hydroxyphenylacetate to hydroquinone. a carboxylic ester + H2O = a carboxylate + an alcohol + H(+) an acetyl ester + H2O = acetate + an aliphatic alcohol + H(+) a butanoate ester + H2O = an aliphatic alcohol + butanoate + H(+) an octanoate ester + H2O = an aliphatic alcohol + H(+) + octanoate decanoate ester + H2O = an aliphatic alcohol + decanoate + H(+) a dodecanoate ester + H2O = an aliphatic alcohol + dodecanoate + H(+) 1,2,3-tributanoylglycerol + H2O = butanoate + dibutanoylglycerol + H(+) 4-acetoxyphenol + H2O = acetate + H(+) + hydroquinone Completely inhibited by tetrahydrolipstatin (THL), RHC-80267 and N-bromosuccinimide. kcat is 7283 min(-1) with pNP-butyrate as substrate. Optimum pH is 8.0. Stable between pH 6.0-9.0 (with pNP-butyrate as substrate). Optimum temperature is 45 degrees Celsius (with pNP-butyrate as substrate). Remains active in dormant M.tuberculosis. Expressed in macrophages after 6 hours of infection. In vitro, expressed only in acidic stress conditions. Belongs to the 'GDXG' lipolytic enzyme family.