Mono-ADP-ribosylates muscle and non-muscle actin. ADP-ribosylates Chinese hamster ovary and HeLa cell actin as well as rabbit muscle, porcine heart actin and non-muscle beta- and gamma-actin. ADP-ribosylation of actin prevents the polymerization of G actin to F actin, causing actin filament depolymerization, destruction of the cytoskeleton and cytotoxicity; this requires only the C-terminal 120 residues. Does not possess NAD(+)-glycohydrolase activity, unlike most mART enzymes. L-arginyl-[protein] + NAD(+) = H(+) + N(omega)-(ADP-D-ribosyl)-L-arginyl-[protein] + nicotinamide Secreted via the type III secretion system 1 (SPI-2 TTSS). A non-polar disruption of this gene is no longer virulent in mouse infection. In Salmonella spp. the spv gene cluster is encoded on a highly transmissible plasmid. Belongs to the SpvB family.