Component of the ESCRT-0 complex which is the sorting receptor for ubiquitinated cargo proteins at the multivesicular body (MVB) and recruits ESCRT-I to the MVB outer membrane (PubMed:3062374, PubMed:1493335, PubMed:9265642, PubMed:11416128, PubMed:12055639, PubMed:11872141, PubMed:12900393, PubMed:14581452, PubMed:15166140, PubMed:15107463, PubMed:15086794, PubMed:17135292, PubMed:18508771). Controls exit from the prevacuolar compartment (PVC) in both the forward direction to the vacuole and the return to the Golgi (PubMed:11208109, PubMed:8649377, PubMed:9015300). Allows VPS10 to return to the (trans-Golgi network) TGN from the PVC (PubMed:8649377). Might also function as an alternate adapter in the COPIb clathrin-like coat (PubMed:17101773). Component of the ESCRT-0 complex composed of HSE1 and VPS27 (PubMed:12055639, PubMed:14581452, PubMed:15086794). Interacts with ENT3 and ENT5, the ESCRT-I subunits VPS23 and VPS28 and with the COPIb subunits SEC27, SEC28 and SEC33 (PubMed:12900393, PubMed:15107463, PubMed:17101773). May form a complex composed of VPS27, HSE1 and DOA1 (PubMed:18508771). Interacts with DOA1 (PubMed:18508771). Interacts with ubiquitin (PubMed:11988742, PubMed:12750381, PubMed:14581452). The FYVE domain is involved in the binding to phosphatidylinositol 3-phosphate (PtdIns(3)P) which is required for the association to endosomal membranes. Both IUM domains are necessary for efficient binding to ubiquitin. Present with 172 molecules/cell in log phase SD medium. Belongs to the VPS27 family.