Degradation of glucosinolates (glucose residue linked by a thioglucoside bound to an amino acid derivative) to glucose, sulfate and any of the products: thiocyanates, isothiocyanates, nitriles, epithionitriles or oxazolidine-2-thiones. a thioglucoside + H2O = a sugar + a thiol. Homodimer. In vacuoles called myrosin grains of a certain class of cells, myrosin cells, distributed in the cotyledons and the axis of the embryo as well as in different organs of the growing plant. It seems that the absence of a catalytic proton donor in plant myrosinases is not impairing the hydrolysis of glucosinolates. Belongs to the glycosyl hydrolase 1 family.