D-aminoacyl-tRNA deacylase with broad substrate specificity. By recycling D-aminoacyl-tRNA to D-amino acids and free tRNA molecules, this enzyme counteracts the toxicity associated with the formation of D-aminoacyl-tRNA entities in vivo. a D-aminoacyl-tRNA + H2O = a D-alpha-amino acid + a tRNA + H(+) glycyl-tRNA(Ala) + H2O = glycine + H(+) + tRNA(Ala) Binds 2 Zn(2+) ions per subunit. Monomer. Belongs to the DtdA deacylase family. Extended N-terminus.