May be required for sperm motility and function. (2R)-2-phosphoglycerate = H2O + phosphoenolpyruvate Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5. Interacts with ENO1 and AKAP4. Synthesized as an approximately 70-kDa precursor, which then undergoes proteolytic cleavage to an approximately 60-kDa enzyme; HOATZ associates directly or indirectly with ENO4 to mediate this process before its transport to mature flagella. Belongs to the enolase family. Although it belongs to the enolase family, Leu-362 is present instead of the conserved Glu which is expected to be an active site residue.