Glycolytic enzyme that catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate. Appears to have a function in striated muscle development and regeneration. (2R)-2-phosphoglycerate = H2O + phosphoenolpyruvate Mg(2+) is required for catalysis and for stabilizing the dimer. Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5. Mammalian enolase is composed of 3 isozyme subunits, alpha, beta and gamma, which can form homodimers or heterodimers which are cell-type and development-specific. Interacts with PNKD (By similarity). Localized to the Z line. Some colocalization with CKM at M-band (By similarity). Belongs to the enolase family.