Catalyzes the transfer of an acetyl group from acetyl-CoA to the 6'-amino group of aminoglycoside molecules conferring resistance to antibiotics containing the purpurosamine ring including amikacin, tobramycin, dibekacin and ribostamycin. Able to acetylate eukaryotic histone proteins. acetyl-CoA + kanamycin B = CoA + H(+) + N(6')-acetylkanamycin B Homodimer. Strain BM4361 does not express or weakly expresses aminoglycoside resistance gene and is thus aminoglycoside-sensitive. Strain BM4362 expresses it due to a chromosomal deletion leading to aminoglycoside resistance.