Hydrolyzes glucose-6-phosphate to glucose in the endoplasmic reticulum. May form with the glucose-6-phosphate transporter (SLC37A4/G6PT) a ubiquitously expressed complex responsible for glucose production through glycogenolysis and gluconeogenesis. Probably required for normal neutrophil function. D-glucose 6-phosphate + H2O = D-glucose + phosphate Inhibited by vanadate. 8 times less active compared to G6PC1 under the same experimental conditions. Carbohydrate biosynthesis; gluconeogenesis. Ubiquitously expressed. Highly expressed in skeletal muscle, at intermediate levels in heart, brain, placenta, kidney, colon, thymus, spleen and pancreas. Also detected in testis, prostate, ovary, liver, lung, small intestine and peripheral blood lymphocytes. The disease is caused by variants affecting the gene represented in this entry. The disease is caused by variants affecting the gene represented in this entry. Belongs to the glucose-6-phosphatase family. According to PubMed:12370122, it has no hydrolytic activity.