This three-finger toxin inhibits ASIC channels. It acts as a gating modifier toxin by decreasing the apparent proton sensitivity of activation and by slightly increasing the apparent proton sensitivity for inactivation (PubMed:23034652). It binds more tightly to the closed state and to a much lesser extent the inactivated/desensitized state of ASIC1a isoform of ASIC1 (PubMed:23034652). It interacts directly with the outside surface of the thumb domain of chicken ASIC1a (ASIC1a), but does not insert into the acidic pocket as suggested for mambalgin-2 (PubMed:29872539). This binding leads to relocation of the thumb domain that could disrupt the acidic pocket of cASIC1a (PubMed:29872539). It reversibly inhibits rat ASIC1a (IC(50)=3.4-55 nM), rat ASIC1a-ASIC2b (IC(50)=61 nM), rat ASIC1a-ASIC1b (IC(50)=72 nM), human ASIC1a (IC(50)=127-580 nM), chicken ASIC1a (IC(50)=123.6 nM), rat ASIC1b (IC(50)=22.2-203 nM), rat ASIC1a-ASIC2a (IC(50)=152-252 nM) (PubMed:23034652, PubMed:23624383, PubMed:25873388, PubMed:24619065, PubMed:26680001, PubMed:29872539). In vivo, it shows a potent naloxone-resistant analgesic effect against acute and inflammatory pain upon central and peripheral injection (PubMed:23034652). In addition, it also has an opioid-independent effect on both thermal and mechanical inflammatory pain after systemic administration and is effective against neuropathic pain (PubMed:26680001). Expressed by the venom gland. Average mass. Promising peptide that shows a potent analgesic effect against acute and inflammatory pain that can be as strong as morphine but resistant to naloxone, with much less tolerance and no respiratory distress. Has no effect on rat ASIC2a, rat ASIC3, rat ASIC1a-ASIC3 and rat ASIC1b-ASIC3 channels, as well as on TRPV1, P2RX2 (P2X2), HTR3A (5-HT3A), Nav1.8 (SCN10A), Cav3.2 (CACNA1H) and Kv1.2 (KCNA2) channels (PubMed:23034652). Does not produce motor dysfunction, apathy, flaccid paralysis, convulsions or death upon central injections (intrathecal or intracerebroventricular) in mice (PubMed:23034652). Belongs to the snake three-finger toxin family. Short-chain subfamily. Mambalgin sub-subfamily.