Involved in oxygen transport from gills to the various peripheral tissues. Hb1 is a heterotetramer of two alpha chains and two beta chains. Red blood cells. This fish has three hemoglobins: Hb1 (major) and two minor hemoglobins (about 1-2% of the total). Hb1 has a strong alkaline Bohr effect, and at low pH exhibits the reduced ligand affinity and cooperativity that comprise the Root effect. Belongs to the globin family.