Cytochrome P4590 monooxygenase part of the gene cluster that mediates the biosynthesis of betaestacins (Ref.3). The bifunctional terpene synthase btcA converts isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) into the sesterterpene betaestacin I (Ref.3). The C-terminal prenyltransferase (PT) domain of btcA catalyzes formation of GFPP, whereas the N-terminal terpene cyclase (TC) domain catalyzes the cyclization of GFPP into betaestacin I (Ref.3). The cytochrome P450 monooxygenase btcB oxidizes the C25 methyl group of betaestacin I to yield the carboxylic acid betaestacin IV via the alcohol betaestacin III (Ref.3). The cytochrome P450 monooxygenase btcC further catalyzes the multistep oxidation of betaestacin IV to produce several compounds, including betaestacins Va, Vb, Vc and VI (Ref.3). Secondary metabolite biosynthesis; terpenoid biosynthesis. Belongs to the cytochrome P450 family.