D-aminoacyl-tRNA deacylase with broad substrate specificity. By recycling D-aminoacyl-tRNA to D-amino acids and free tRNA molecules, this enzyme counteracts the toxicity associated with the formation of D-aminoacyl-tRNA entities in vivo. Catalyzes the hydrolysis of D-tyrosyl-tRNA(Tyr) and D-aspartyl-tRNA(Asp). a D-aminoacyl-tRNA + H2O = a D-alpha-amino acid + a tRNA + H(+) glycyl-tRNA(Ala) + H2O = glycine + H(+) + tRNA(Ala) D-tyrosyl-tRNA(Tyr) + H2O = D-tyrosine + tRNA(Tyr) Binds 2 Zn(2+) ions per subunit. Thermostable. Monomer. Belongs to the DtdA deacylase family. Extended N-terminus.