Converts cob(I)alamin to adenosylcobalamin (adenosylcob(III)alamin), the cofactor for propanediol dehydratase. Found in the bacterial microcompartment (BMC) dedicated to 1,2-propanediol (1,2-PD) degradation (PubMed:11160088, PubMed:15547259, PubMed:27446048, PubMed:15817784, PubMed:20656910). For adenosylcobalamin synthesis dATP can replace ATP, but no other nucleotides will substitute (PubMed:15547259). PduS and PduO allow regeneration of the adenosylcobalamin cofactor within the BMC (Probable). The 1,2-PD-specific bacterial microcompartment (BMC) concentrates low levels of 1,2-PD catabolic enzymes, concentrates volatile reaction intermediates thus enhancing pathway flux and keeps the level of toxic, mutagenic propionaldehyde low. ATP + cob(I)alamin-[corrinoid adenosyltransferase] = adenosylcob(III)alamin + apo-[corrinoid adenosyltransferase] + triphosphate Stoichiometry of heme binding is 2 PduOC:1 heme; the binding pocket is formed by a PduOC dimer. Full-length PduO also binds heme. Mn(2+) and Co(2+) are almost as active as Mg(2+) for adenosylcobalamin synthesis. Inhibited by ADP but not significantly by other nucleotides, inhibited by diphosphate and less well by triphosphate. Polyol metabolism; 1,2-propanediol degradation. Cofactor biosynthesis; adenosylcobalamin biosynthesis. The C-terminal domain (PduOC) forms stable octomers and also crystallizes as an octomer (PubMed:27446048). Forms a complex with PduS (PubMed:15817784, PubMed:20656910). BMC production is induced by growth on 1,2-PD vitamin B12 medium. The N-terminus (PduON, residues 1-185) has adenosyltransferase activity in vivo and in vitro; it partially complements a deletion mutant for growth on 1,2-PD (PubMed:15547259, PubMed:27446048). The C-terminal domain (PduOC, residues 194-336) is required for full complemention of a pduO deletion (PubMed:27446048). Decreased growth on 1,2-PD cyanocobalamin medium; no growth is seen in a double cobA-pduO deletion. Bacterial microcompartments (BMC) 100-200 nm in cross section are formed during aerobic growth on minimal 1,2-PD-B12 or anaerobic growth on 1,2-PD-tetrathionate medium, but not during aerobic growth on glucose, anerobic growth on glucose or pyruvate-tetrathionate (PubMed:10498708). BMCs can constitute up to 10% of total cell protein (PubMed:12923081). Belongs to the Cob(I)alamin adenosyltransferase family. PduO subfamily.