Plays a major role in RD1-associated pathogenesis, and may contribute to the establishment and maintenance of M.tuberculosis infection. Together with PPE68, stimulates the secretion of IL-10 and MCP-1 from human macrophages, via the interaction with human Toll-like receptor 2 (TLR2). Interacts with PPE68. PE35/PPE68 complex interacts with human TLR2. Inactivation impairs expression of EsxA (ESAT-6) and EsxB (CFP-10). Could be used for serodiagnosis of both pulmonary and extra-pulmonary tuberculosis. Belongs to the mycobacterial PE family.