After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. The channel is blocked by alpha-bungarotoxin (By similarity). Homopentamer. Interacts with RIC3; which is required for proper folding and assembly. Interacts with LYPD6. Interacts with the alpha-conotoxin RgIA. Interacts with alpha-conotoxins ImI and ImII (By similarity). Interacts with CANX (By similarity). TMEM35A/NACHO and RIC3 promote its trafficking to the cell membrane. Glycosylations at Asn-46, Asn-90 and Asn-133 are essential for TMEM35A/NACHO-mediated proper subunit assembly and trafficking to the cell membrane. Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Alpha-7/CHRNA7 sub-subfamily.