This recombinant serine protease inhibitor inhibits trypsin (Ki=30 nM) (Ref.3). It possesses anti-inflammatory activity in vitro (Ref.3). It blocks histamine influence on intracellular calcium concentration in murine bone marrow-derived macrophages (62.2% inhibition at 10 uM), which can indicate inhibition of H1-histamine receptor (HRH1) (Ref.3). In contrast to some paralogs, this protein decreases reactive oxygen species (ROS) level in the oxidative stress agent 6-hydroxydopamine (6-OHDA)-induced neurotoxicity model, but does not show cytoprotective activity on neuroblastoma cells (PubMed:33802055). In vivo, it shows analgesic activity, since it increases hot plate and tail flick withdrawal latencies, when using a mice thermal pain stimulation model (PubMed:25937220). Has no activity on Kv1.1/KCNA1, Kv1.2/KCNA2, Kv1.3/KCNA3, Kv1.4/KCNA4, Kv1.5/KCNA5, Kv1.6/KCNA6, Shaker, Kv11.1/KCNH2/ERG1 potassium channels and on TRPV1, the capsaicin receptors. Belongs to the venom Kunitz-type family. Sea anemone type 2 potassium channel toxin subfamily.