In addition to acetyl-CoA (100%), the enzyme accepts propionyl-CoA (60%) and butyryl-CoA (30%). acetyl-CoA + phosphate = acetyl phosphate + CoA Optimum temperature is 90 degrees Celsius. Metabolic intermediate biosynthesis; acetyl-CoA biosynthesis; acetyl-CoA from acetate: step 2/2. Homotetramer. Belongs to the phosphate acetyltransferase and butyryltransferase family.