Seed storage protein. Homotrimer (PubMed:21861454). Hexamer (PubMed:20362338). Each subunit is composed of an acidic and a basic chain derived from a single precursor and linked by a disulfide bond (By similarity). Expressed in seed (at protein level) (PubMed:14555856, PubMed:18558706, PubMed:18795784, PubMed:20362336, PubMed:21138244, PubMed:25766831, PubMed:26769082, PubMed:24926808, PubMed:28959544, PubMed:27513566). Expressed in the juice of the cashew apple (at protein level) (PubMed:18558706). Expressed during seed maturation (PubMed:14555856). Expressed in mature raw cashew nut (PubMed:18558706, PubMed:21138244). Proteolytically processed from a single precursor to produce an acidic and a basic chain that are linked by a disulfide bond (PubMed:14555856, PubMed:20362336, PubMed:24926808, PubMed:26769082). Not glycosylated (PubMed:26769082). Causes an allergic reaction in human (PubMed:14555856, PubMed:18558706, PubMed:20362336, PubMed:24926808, PubMed:25766831, PubMed:27129138, PubMed:26769082, PubMed:27513566). Binds to IgE of patients allergic to cashew nuts (PubMed:14555856, PubMed:18558706, PubMed:20362336, PubMed:24926808, PubMed:25766831, PubMed:26769082, PubMed:27513566). Recombinant protein binds to IgE in 62% of the 21 patients tested (PubMed:14555856). Reduced IgE-binding following 50 mM sodium sulfite treatment at 100 degrees Celsius or by 5 mM dithiothreitol (DTT) (PubMed:24926808). IgE-binding is reduced by 35% with 5 mM sodium oleate treatment at 70 degrees Celsius for 60 min only if followed by an additional overnight incubation at 37 degrees Celsius (PubMed:25766831). Allergenicity is removed from the cashew apple juice concentrate by 5 kDa filtration (PubMed:18558706). Retains immunoreactivity for mouse monoclonal antibodies (mAbs) 4C3 and 4H9 after a variety of processing treatments including autoclaving, blanching, microwave heating, dry roasting, gamma-irradiation and pH (PubMed:18795784). Significantly reduced immunoreactivity for mouse mAb 4C3 by 2.5 mM sodium dodecyl sulfate (SDS) or 2% v/v reducing agent beta-mercaptoethanol (beta-ME) with heat (100 degrees Celsius for 10 min) treatments (PubMed:21138244). Exposure to extreme pH (1 or 13) and extreme heat treatments (autoclaving for 30 min or roasting at 200 degrees Celsius for 15 min) results in almost complete loss of binding to mouse mAb 4C3 (PubMed:18795784). Mouse mAb 2B5 recognizes a conformational epitope on the acidic chain of this protein, the recognition of which requires the association of the basic chain, but is independent of their post-translational cleavage. The antibody competes with patient IgE for binding to the epitope. The epitope is destroyed by physical (boiling) and chemical (0.5 M beta-ME, 10% SDS and 6 M urea) denturation (PubMed:20362336). Cashew allergic patients elicit responses to this protein by CD4(+) T cells with T-helper 2 (Th2) and Th2/T-helper 17 (Th17) phenotypes (PubMed:27129138). Can be used as part of the diagnostics for predicting the risk for positive double-blind, placebo-controlled food challenge test (DBPCFC) in cashew-allergic children. The risk increases with higher specific IgE levels to this protein. Belongs to the 11S seed storage protein (globulins) family.