Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. an organic molecule + O2 + reduced [NADPH--hemoprotein reductase] = an alcohol + H(+) + H2O + oxidized [NADPH--hemoprotein reductase] Interacts with chaperones HSP70 and HSP90; this interaction is required for initial targeting to mitochondria. Post-translationally targeted to mitochondria. Requires the cytosolic chaperones HSP70 and HSP90, for initial targeting to mitochondria, and then requires all three of the receptor proteins in the TOM complex, TOMM70, TOMM20 and TOMM22 for translocation across the mitochondrial outer membrane. After translocation into the matrix, associates with the inner membrane as a membrane extrinsic protein. By phenobarbital. Phosphorylation is accompanied by a decrease in enzyme activity. Belongs to the cytochrome P450 family.