Catalyzes the aldol condensation of dihydroxyacetone phosphate (DHAP or glycerone-phosphate) with glyceraldehyde 3-phosphate (G3P) to form fructose 1,6-bisphosphate (FBP) in gluconeogenesis and the reverse reaction in glycolysis. Is required for the utilization of fructose as a sole carbon and energy source. Plays a role in gluconeogenesis during growth on acetate, D-xylose, and casamino acids. beta-D-fructose 1,6-bisphosphate = D-glyceraldehyde 3-phosphate + dihydroxyacetone phosphate Binds 2 Zn(2+) ions per subunit. One is catalytic and the other provides a structural contribution. EDTA is an inhibitor of catalytic activity. Optimum pH is 7.5. Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 4/4. Homodimer. Expression is highly up-regulated in presence of fructose. Loss of growth on fructose, but growth on glucose is slightly stimulated. Loss of growth on acetate, D-xylose, or Casamino acids. Belongs to the class II fructose-bisphosphate aldolase family.