Protein kinase that plays a role in vesicle-mediated transport processes and exocytosis. Can phosphorylate CCNY at 'Ser-336' (in vitro) Plays a role in the regulation of insulin secretion in response to changes in blood glucose levels. Regulates GH1 release by brain neurons. Required for normal spermatogenesis. Regulates neuron differentiation and dendrite development (By similarity). Phosphorylates NSF, and thereby regulates NSF oligomerization. ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-[protein] ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-threonyl-[protein] Interacts with BRSK2. Interacts with CCNY; this increases the CDK16 kinase activity (By similarity). Found in a complex containing CABLES1, CDK17 and TDRD7. Interacts with YWHAH, YWHAQ and YWHAZ. Interacts with NSF. Identified in a complex with NSF, syntaxin-1, synaptotagmin, SYN1, SYP and CDK5R1. Colocalizes with insulin in pancreas islets. Detected in brain (at protein level). Isoform 1 and isoform 2 are expressed in brain, kidney, lung, heart and lens fiber. Phosphorylation at Ser-153 inhibits kinase activity. Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.