This enzyme is an effector of chloramphenicol resistance in bacteria. acetyl-CoA + chloramphenicol = chloramphenicol 3-acetate + CoA Homotrimer. Type II chloramphenicol acetyltransferases are sensitive to inhibition by thiol-reactive reagents. The inactivation occurs as a result of chemical modification of Cys-26. Belongs to the chloramphenicol acetyltransferase family.