Alpha-conotoxins act on postsynaptic membranes, they bind to the nicotinic acetylcholine receptors (nAChR) and thus inhibit them. At 1 uM, the synthetic peptide reversibly blocks mammalian nAChR alpha-1/beta-1/gamma/delta and, to a lesser degree, alpha-6/alpha-3/beta-2, but has no effect on alpha-7. At 10 uM, it reversibly blocks nAChR alpha-3/beta-2, but has no effect on nAChR alpha-3/beta-4, alpha-4/beta-2, alpha-4/beta-4 or alpha-6/alpha-3/beta-4. Intramuscular injection of 10-20 ug of the synthetic peptide in goldfish causes death. Intravenous injection of the same doses in Kunming male mice causes unconsciousness. Does not inhibit potassium channels. Expressed by the venom duct. The cysteine framework is XIV (C-C-C-C). Belongs to the conotoxin A superfamily.