Nucleosome-destabilizing factor that is recruited to genes during transcriptional activation and colocalizes with a subset of trimethylated 'Lys-36' histone H3 (H3K36me3)-enriched regions (PubMed:23295261, PubMed:29759984). Binds DNA (in vitro) (PubMed:29759984). Facilitates Pol II transcription through nucleosomes (PubMed:29759984). Facilitates male-specific lethal (MSL) histone acetyltransferase complex targeting to active genes on the X chromosome (PubMed:23295261). Stimulates the acetylation of 'Lys-56' of nucleosomal histone H3 (H3K56ac) by nej (PubMed:29759984). May have oxidoreductase activity (By similarity). Binds to mononucleosomes (PubMed:29759984). Interacts with male-specific lethal (MSL) histone acetyltransferase complex at least composed of mof, msl-1, msl-2 and msl-3 (PubMed:23295261). Localization to open chromatin depends on H3K36 trimethylation by Set2. The PWWP domain is a H3 reader and strongly binds DNA. In the dehydrogenase domain, the conserved NAD(P)H-binding sites and sequence similarity to plant dehydrogenases suggest that this protein may have oxidoreductase activity. However, since the active site is not conserved, the dehydrogenase domain seems to serve as a catalytically inert oligomerization module. RNAi-mediated knockdown results in ectopic MSL-binding sites on autosomes of male larvae. Belongs to the HIBADH-related family. NP60 subfamily.