Seems to play a role in the correct specification of the left-right axis. May antagonize NODAL and BMP4 signaling. Cystine knot-containing proteins play important roles during development, organogenesis, and tissue growth and differentiation. At 7.0 dpc (early head-fold stage) expressed in perinodal region. At 7.5 dpc (late head-fold stage) expression begins to decrease in intensity on the left side and starts to be asymmetrically up-regulated on the right side of the node. By early 8.0 dpc (somitogenesis), highly expressed in the right side of the node. No expression seen in later stages of development. Mice display multiple laterality defects including randomization of the L/R axis, left pulmonary isomerism thoracic situs inversus and cardiovascular malformations. Belongs to the DAN family.