E3 ubiquitin-protein ligase. Regulates the activity of daf-16 and is thereby involved in regulating aging and stress resistance (PubMed:17276341). Regulates nsy-1 activity and thereby attenuates the activation of sek-1 and pmk-1, two components of the p38 pathway, which results in susceptibility to pathogenic bacterial infection (PubMed:24448648). S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine. Protein degradation; proteasomal ubiquitin-dependent pathway. Interacts (via C-terminus) with daf-16. Increased lifespan, retarded growth and development (PubMed:17276341). Increased survival rate upon P.aeruginosa infection (PubMed:24448648).