Acetyltransferase; part of the gene cluster that mediates the biosynthesis of pleuromutilin, a tricyclic diterpene showing antibacterial properties (PubMed:28924980). The geranylgeranyl diphosphate (GGPP) synthase ple4 catalyzes the first step in pleuromutilin biosynthesis (PubMed:28924980). GGPP is then substrate of the premutilin synthase (PS) ple3 to yield premutilin (PubMed:28924980). Premutilin synthase is a bifunctional enzyme composed of the fusion of a class II diterpene cyclase (DTC) and a class I diterpene synthase (DTS), with the corresponding domains and active sites containing characteristic aspartate-rich motifs (By similarity). GGPP is first converted to mutildienyl-diphosphate (MPP) at the class II DTC site (By similarity). MPP is subsequently further cyclized at the class I DTS site, followed by a 1,5-hydride shift and addition of water prior to terminating deprotonation, to yield premutilin (By similarity). The cytochrome P450 monooxygenases ple5 and ple6 hydroxylate premutilin at C-11 and C-3, respectively, producing 11-hydroxypremutilin and 3-hydroxypremutilin (PubMed:28924980). The combination of the actions of both ple5 and ple6 leads to the production of 3,11-dihydroxypremutilin (PubMed:28924980). The short chain dehydrogenase ple7 further converts 3,11-dihydroxypremutilin into mutilin (PubMed:28924980). The acetyltransferase ple2 then acetylates mutilin to produce 14-O-acetylmutilin (PubMed:28924980). Finally, the cytochrome P450 monooxygenase ple1 catalyzes hydroxylation on the alpha position of the acetyl side chain of 14-O-acetylmutilin to yield pleuromutilin (PubMed:28924980). Secondary metabolite biosynthesis; terpenoid biosynthesis. Belongs to the wax synthase family.