Positive effector of the Wnt signaling pathway; activates WNT3A signaling via DVL2. Regulates JNK activation by AXIN1 and DVL2. May bind filamentous actin. Directly interacts (via DIX domain) with DVL2 (via DIX domain). Interacts with gamma-tubulin (By similarity). Interacts with the complex composed of DVL2 and Rac. Interacts with AXIN1; competes with MAP3K1. Interacts with MAP3K4 preventing MAP3K4 interaction with AXIN1. Colocalizes with gamma-tubulin at the centrosome, both during interphase and mitosis. Associated with actin stress fiber at the filament ends. Abundantly expressed in brain and testis and to a lower extent in lung, kidney, colon, ovary and urinary bladder. Expressed in brain, liver, testis and spleen (at protein level). Expressed throughout the brain with strong expression in main and accessory olfactory bulbs, cerebral cortex, piriform cortex, hippocampus, habenular nucleus, dorsal thalamus, superior and inferior colliculi and cerebellum. Expressed in embryonic brain, liver and spleen (at protein level). First detected at 7.5 dpc in the node. Expressed in the cephalic mesenchyme and tail bud at 8.5 dpc, and in the branchial arch and forelimb bud at 9.5 dpc. In the central nervous system, expression begins and persists in the regions where the neurons differentiate. Expression is also strong in the peripheral nervous system, including sensory cranial ganglia, dorsal root ganglia, and autonomic ganglia, and in the sensory organs, such as the inner nuclear layer of the neural retina, saccule and cochlea of the inner ear, and nasal epithelium. Outside the nervous system, expression is detected in the cartilage, tongue, lung bud, stomach, and gonad from 12.5 dpc to 14.5 dpc, and in the tooth bud, bronchial epithelium, and kidney at 17.5 dpc. The coiled-coil domain mediates interaction with MAP3K4 and inhibition of AXIN1-mediated JNK activation through MAP3K4. The DIX domain mediates interaction with AXIN1 and inhibition of AXIN1-mediated JNK activation through MAP3K1. Mediates interaction with DVL2; this interaction is required for activation of Wnt signaling (By similarity). Phosphorylated on tyrosine and serine residues. Polyubiquitinated, leading to its proteasomal degradation. WNT3A signaling increases DIXDC1 protein levels by inhibiting its ubiquitination and subsequent degradation. Major isoform. Major isoform. Major isoform. Belongs to the DIXDC1 family.