ATP + pyruvate = ADP + H(+) + phosphoenolpyruvate Not activated by classical allosteric effectors. Optimum pH is 6.1. Optimum temperature is higher than 95 degrees Celsius. Still active after 120 minutes at 100 degrees Celsius. Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5. Homotetramer. Belongs to the pyruvate kinase family.