Peroxisomal citrate synthase involved in the citrate homeostasis (PubMed:3023912, PubMed:25982115). Catalyzes the condensation of acetyl coenzyme A and oxaloacetate to form citrate (PubMed:3023912, PubMed:25982115). Citrate synthase is the rate-limiting enzyme of the tricarboxylic acid (TCA) cycle (Probable). acetyl-CoA + H2O + oxaloacetate = citrate + CoA + H(+) Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate from oxaloacetate: step 1/2. Interacts with F-box protein UCC1. Expression is repressed in medium containing both glucose and glutamate (PubMed:3023912). Expression is up-regulated by the presence of C2-compounds such as acetate (PubMed:25982115). Ubiquitinated by the E3 ubiquitin-protein ligase complex SCF(UCC1), which leads to its degradation by the proteasome (PubMed:25982115). Ubiquitination is prevented by oxaloacetate, suggesting the existence of an oxaloacetate-dependent positive feedback loop that stabilizes CIT2 (PubMed:25982115). Leads to glutamate auxotrophy and poor growth on rich medium containing lactate, a nonfermentable carbon source, when CIT1 is also deleted. Citrate synthase is found in nearly all cells capable of oxidative metabolism. Present with 2310 molecules/cell in log phase SD medium. Belongs to the citrate synthase family.