Component of a binary insecticidal toxin active on western corn rootworm (WCR, Diabrotica virgifera subsp. virgifera Le Conte) and probably also on northern corn rootworm (D.barberi). Both proteins are required for maximal toxicity. The larval midgut epithelium is probably the primary target (PubMed:11433280, PubMed:11872461). This protein alone has no activity against southern corn rootworm (Diabrotica undecimpunctata howardi Barber), but it synergizes the toxic effect of its Cry34Ab1 partner (PubMed:12076012). The 2 proteins individually and together form ion channels; channels made in the presence of the 2 proteins have higher conductance (PubMed:15379574). Binds to WCR third instar midgut brush border membrane vesicles; binding improves over 10-fold in the presence of Cry34Ab1 (PubMed:23308139). Monomer in solution (PubMed:25390338). Copurifies from parasporal inclusion bodies with Cry34Ab1 (PubMed:11433280, PubMed:11872461, PubMed:15379574). Expressed upon sporulation. Probably part of the cry34Ab1-cry35Ab1 operon. Proteolytic processing occurs near the C-terminus yielding a stable protein of approximately 40 kDa; this may be the active form of the protein. Expression of the 2 toxin genes in transgenic corn confers resistance to WCR and has been commercialized. Belongs to the toxin_10 family.