A cholesterol-dependent toxin that causes cytolysis by forming pores in cholesterol containing host membranes (PubMed:6411708). After binding to target membranes, the protein undergoes a major conformation change, leading to its insertion in the host membrane and formation of an oligomeric pore complex. Cholesterol is required for binding to host cell membranes, membrane insertion and pore formation; cholesterol binding is mediated by a Thr-Leu pair in the C-terminus. Can be reversibly inactivated by oxidation (By similarity). Inhibited by cholesterol and thiol reagents. Homooligomeric pore complex of 35 to 50 subunits; when inserted in the host membrane. Secreted as soluble protein that then inserts into the host cell membrane and forms pores formed by transmembrane beta-strands. Belongs to the cholesterol-dependent cytolysin family.