Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Serves as a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis. Regulates apical junction formation in bronchial epithelial cells. Interacts with RTKN (By similarity). Interacts with AKAP13 (PubMed:11546812). Interacts with DIAPH1 (PubMed:15864301). Interacts with PKN2 (PubMed:20974804). Interacts with ROCK1 and ROCK2 (PubMed:8816443). Interacts with ARHGDIA (PubMed:20400958). Interacts with RIPOR1 (PubMed:27807006). Translocates to the equatorial region before furrow formation in a ECT2-dependent manner. (Microbial infection) Glycosylated at Tyr-34 by Photorhabdus asymbiotica toxin PAU_02230. Mono-O-GlcNAcylation by PAU_02230 inhibits downstream signaling by an impaired interaction with diverse regulator and effector proteins of Rho and leads to actin disassembly. (Microbial infection) Glucosylated at Thr-37 by C.difficile toxins TcdA and TcdB in the colonic epithelium (PubMed:24905543). Monoglucosylation completely prevents the recognition of the downstream effector, blocking the GTPases in their inactive form, leading to actin cytoskeleton disruption (PubMed:24905543). Belongs to the small GTPase superfamily. Rho family.