Pleiotropic ABC efflux transporter that confers resistance to numerous chemicals including anisomycin, cycloheximide, fluconazole, miconazole, ketoconazole, itriconazole, nystatin, terbinafine, amorolfine, brefeldin A, amphotericin B, fluphenazine, as well as estrogen. Plays a role in farnesol-induced apoptotic process through glutathione efflux activity. Mediates in-to-out translocation of membrane phospholipids including aminophospholipids and thus regulates asymmetric distribution of phosphatidylethanolamine. Exhibits nucleoside triphosphatase activity. Disulfiram reverses CDR1-mediated drug resistance by interaction with both ATP and substrate-binding sites of the transporter and may be useful for antifungal therapy. Accumulates at membrane rafts. Both ergosterol and sphingolipids are essential for targeting to membrane rafts. Mislocalizes to the vacuole in absence of FZO1. The distal promoter mediates the miconazole response whereas the proximal promoter (-345/+1) contains all the regulatory domains required for its induction by various other stresses. The promoter also contains a steroid responsive region (SRR) conferring beta-oestradiol and progesterone inducibility. Transcription is positively regulated by NCB2, NTD80 and TAC1 and repressed by FCR1. Expression is up-regulated during biofilm development, by heat-shock, and by benomyl, doxorubicin, miconazole, vinblastine, adriamycin, fluphenazine, cycloheximide, calcofluor, canavanine, 5'-fluorcytosine, cilofungin and caffeine. Expression is repressed by serum and inhibited by tetrandrine. Transcription is also reduced during in vitro fluconazole exposure but in the postexposure period, the mRNA abundance increases. Contains 2 cytoplasmic nucleotide binding domains (NBDs). The N-terminal NBD has ATPase activity. The 2 NBDs are asymmetric and non-exchangeable and the drug efflux by CDR1 involves complex interactions between the two halves of the protein. Leads to hypersusceptibility to the antifungal agents terbinafine and amorolfine, and to the metabolic inhibitors cycloheximide, brefeldin A, and fluphenazine. Shows a decreased number of germ tube-forming cells in the presence of estradiol when CDR2 is also deleted. Belongs to the ABC transporter superfamily.