Component of a complex that catalyzes the oxidation of glycolate to glyoxylate. Is also able to oxidize D-lactate ((R)-lactate). Does not link directly to O(2), and 2,6-dichloroindophenol (DCIP) and phenazine methosulfate (PMS) can act as artificial electron acceptors in vitro, but the physiological molecule that functions as primary electron acceptor during glycolate oxidation is unknown. A + glycolate = AH2 + glyoxylate (R)-lactate + A = AH2 + pyruvate The glycolate oxidase likely consists of several subunits including GlcD and GlcF. Belongs to the FAD-binding oxidoreductase/transferase type 4 family.