Oxidizes strain chain acyl-CoAs with a chain length of 10 to 14 carbons. Also active toward the 2S isomers of acyl-CoA-esters containing a 2-methyl group. a 2,3-saturated acyl-CoA + O2 = a (2E)-enoyl-CoA + H2O2 Lipid metabolism; peroxisomal fatty acid beta-oxidation. Heteropentamer composed of five different subunits. Belongs to the acyl-CoA oxidase family.