Component of the kinetochore, a multiprotein complex that assembles on centromeric DNA and attaches chromosomes to spindle microtubules, mediating chromosome segregation and sister chromatid segregation during meiosis and mitosis. Component of the inner kinetochore constitutive centromere-associated network (CCAN), which serves as a structural platform for outer kinetochore assembly. Component of the heterotrimeric kinetochore subcomplex CTF3, which consists of CTF3, MCM16 and MCM22 (PubMed:11782448). The CTF3 subcomplex is part of a larger constitutive centromere-associated network (CCAN) (also known as central kinetochore CTF19 complex in yeast), which is composed of at least AME1, CHL4, CNN1, CTF3, CTF19, IML3, MCM16, MCM21, MCM22, MHF1, MHF2, MIF2, NKP1, NKP2, OKP1 and WIP1 (PubMed:12408861, PubMed:22561346). Interacts with CTF19 (PubMed:11782448). Associated with kinetochores. Present with 279 molecules/cell in log phase SD medium. Belongs to the CENP-H/MCM16 family.