Catalyzes the pyridoxal 5'-phosphate-dependent transamination of alanine with glyoxylate as an amino group acceptor (PubMed:16681462). Can also catalyze, although with much less efficiency, the transamination of serine, and histidine with glyoxylate and the and transamination of glycine with pyruvate as an amino group acceptor (PubMed:16681462). Does not catalyze the transamination of both 3-hydroxykynurenine and L-kynurenine (PubMed:16681462). May play a role in the detoxification of glyoxylate, a toxic plant metabolite from the pupae and adult diet (Probable). glyoxylate + L-alanine = glycine + pyruvate kcat is 117 sec(-1) for alanine (at 45 degrees Celsius, pH 7.5 and with glyoxylate as cosubstrate). Optimum pH is 9 (PubMed:16681462). Active between pH 7-10 (for alanine and glyoxylate as substrates) (PubMed:16681462). Optimum temperature is 60 degrees Celsius (PubMed:16681462). Maximum activity between 50 and 80 degrees Celsius (for alanine and glyoxylate as substrates) (PubMed:16681462). Homodimer. Expressed in head and abdomen of adult females. Expressed at pupal and adult stages (at protein level). Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family.