(2R)-2-phosphoglycerate = H2O + phosphoenolpyruvate Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5. Belongs to the enolase family. Although it belongs to the enolase family, Leu-332 is present instead of the conserved Glu which is expected to be an active site residue.