D-glyceraldehyde 3-phosphate + NAD(+) + phosphate = (2R)-3-phospho-glyceroyl phosphate + H(+) + NADH Inhibited by koningic acid through the interaction of cysteine residues with koningic acid even at very low concentrations. Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5. Homotetramer. This protein is a koningic acid (antibiotic)-resistant GAPDH isozyme. It is present under antibiotic production. Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.