ATP + pyruvate = ADP + H(+) + phosphoenolpyruvate Activated by fructose 2,6-bisphosphate, activated by the effector in a cooperative manner. Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5. Homotetramer. Belongs to the pyruvate kinase family.