High affinity molybdate transporter. Unable to transport sulfate. Not inhibited by sulfate. (PubMed:18454190) shows a localization in the mitochondrial membrane while (PubMed:18003916) shows a plasma membrane and endomembrane system localization. Strongly expressed in roots. Detected in the vascular tissues of hypocotyls, in petioles and vascular tissues of cotyledons and leaves, in mesophyll cells, stamen, sepals and siliques. Not regulated during leaf senescence. 90% and 80% reduction in molybdate content in shoots and roots respectively. Reduced growth of roots and shoots under conditions of limited molybdate supply. 9-fold reduction in molybdate content in leaf vacuoles. Decreased nitrate reductase activity due to a reduced molybdate uptake. The amino-acid substitution Asp-429 to Val observed in cv. Landsberg erecta may decrease the molybdate transport activity. A 54-bp deletion in the promoter of MOT1 is strongly associated with the decreased expression of the gene and the resulting low molybdate content observed in several cultivars. Belongs to the SLC26A/SulP transporter (TC 2.A.53) family.