Toxin that may interact with target cell membranes, producing a concentration-dependent leak in ion conductance, possibly via multimeric pore formation (Probable). It produces an immediate sharp increase of calcium concentration in all DRG neurons (PubMed:30214940). This influx in calcium stabilizes without resulting in any observable dye leakage, showing that the effect is not simply cytolytic (PubMed:30214940). This toxin may be one of the major contributors to the pain associated with envenomation (PubMed:30214940). The toxin also displays a weak cytotoxicity (on HEK cells) and some antimicrobial activity (MIC=2.5 uM on C.neoformans (var. grubii), MIC=10.2 uM on S.aureus), but is not hemolytic to human erythtrocytes (PubMed:30214940). In vivo, intraplantar injection into mice causes spontaneous nocifensive behavior (licking, flinching, or shaking of the paw), which lasts 5-7 minutes (10 and 100 uM tested). Mechanical and heat hypoalgesia are observed at 20 and 25 minutes after injection (highest dose tested of 100 uM) (PubMed:30214940). In vivo, injection into crickets (A.domesticus) causes an immediate, dose-dependent, reversible and nonlethal incapacitation that lasts about 53 minutes at the highest dose tested (60 ug/g) (PubMed:30214940). Expressed by the venom gland (PubMed:30214940). This toxin is detected along the entire venom gland, as well as in the venom reservoir, the venom duct and in the venom. No toxin are detected in the Dufour's gland (PubMed:30214940). Does not act directly on human Nav1.7/SCN9A voltage-gated sodium channels. Belongs to the formicidae venom precursor-01 superfamily.