Component of an extracellular matrix cue that guides dorsoventral migrations on the epidermis (PubMed:8861903). Required for the guidance of pioneer axons and migrating cells along the body wall (PubMed:8332188, PubMed:28846083). In particular, it is required for the guidance of axons from neurons, including SubL neurons and AIY interneurons, into the nerve ring (PubMed:28846083, PubMed:9473333). During gonad morphogenesis, involved in distal tip cell (DTC) migration from the dorsal side of the hermaphrodite body to the midbody to allow for formation of gonad arms (PubMed:22732572, PubMed:17716643). Its association with either unc-40 or unc-5 receptors will lead to axon attraction or repulsion, respectively (PubMed:8332188, PubMed:8861903, PubMed:9473333). Involved in dendritic morphogenesis; may act by association with unc-40 at the tips of growing dendrites for interaction with unc-5 on the apposing branch to induce mutual repulsion (PubMed:22426253). Involved in the positioning of ray 1, the most anterior ray sensilium, in the male tail (PubMed:24004945). Required for the formation of synapses between the AVA interneurons and the PHB sensory neurons (PubMed:29742100). Binds to unc-5 and unc-40 receptors. Epidermal basal lamina. Mutations that delete the second laminin EGF-like repeat impair only dorsal migrations of mesodermal cells and axons. Defective gonad distal tip cell (DTC) migration whereby DTCs migrate towards the midbody of the hermaphrodite on the ventral side rather than the dorsal side of the body resulting in ventrally located gonad arms. Double knockout with mig-38 RNAi results in failed gonad DTC migration to the midbody of the hermaphrodite.