Oxidizes the CoA-esters of 2-methyl-branched fatty acids. a 2,3-saturated acyl-CoA + O2 = a (2E)-enoyl-CoA + H2O2 (2S)-pristanoyl-CoA + O2 = (2E)-pristenoyl-CoA + H2O2 O2 + tetracosanoyl-CoA = (2E)-tetracosenoyl-CoA + H2O2 hexadecanoyl-CoA + O2 = (2E)-hexadecenoyl-CoA + H2O2 hexadecanedioyl-CoA + O2 = (2E)-hexadecenedioyl-CoA + H2O2 Lipid metabolism; peroxisomal fatty acid beta-oxidation. Belongs to the acyl-CoA oxidase family.