Probably plays a role in resistance to quinolone antibiotics (PubMed:16870791). Only inhibits ATP-dependent DNA supercoiling by E.coli gyrase at high concentration (30 uM) (PubMed:19060136). Protects E.coli gyrase supercoiling activity from inhibition by fluoroquinolones (ciprofloxacin) at 0.1 uM, does not protect M.tuberculosis gyrase activity (PubMed:19060136). The gene for this protein has been isolated from plasmids in multiple Enterobacteriaceae (PubMed:16870791, PubMed:19060136). For experiments in situ it was cloned from E.cloacae strain HM05-184 but tested in E.coli (PubMed:19060136). Belongs to the pentapeptide repeat protein family.