(S)-malate + NADP(+) = CO2 + NADPH + pyruvate H(+) + oxaloacetate = CO2 + pyruvate Divalent metal cations. Prefers magnesium or manganese. Activated by coenzyme A (CoA), aspartate, succinate and fumarate. Repressed by oxaloacetate, glucose and ATP. kcat is 324.1 sec(-1) with NADP and malate as substrates (at pH 7.5). kcat is 75 sec(-1) with pyruvate as substrate (at pH 7). Optimum pH is 6.8. Homohexamers and homooctamers. Expressed in leaves, stems, flowers and roots. Particularly present in vasculatures, trichome basal cells and hydatodes. During embryogenesis, present in the embryo at the globular and heart stages. Detected in the funiculus and vascular tissues of the siliques. During germination, restricted to the proximal part of the radicle having root hairs to later expands toward the meristematic region, except for the root tip. Strongly expressed in hypocotyls and cotyledons 6 days after imbibition. Present in primary leaves. In developed flowers expressed in sepals and filaments. In developing siliques, present at both ends, the stigmatic papillae and the abscission zone. Belongs to the malic enzymes family.