The molecular target of Acra3 remains undiscovered. In vivo, induces severe neurotoxic events in mice such as excitability and convulsions, leading to the death of the animals within a few minutes after injection. Does not show toxicity in insects and crustaceans (PubMed:22819772). Exerts very strong cytotoxic effect on a mouse brain tumor cell line (BC3H1) with an IC(50) value of 5 mg/ml. It exerts its effects by inducing a stronger necrosis than apoptosis in BC3H1 cells (PubMed:24055552). Expressed by the venom gland. Has the structural arrangement of an alpha-helix connected to antiparallel beta-sheets by disulfide bonds (CS-alpha/beta). LD(50) is less than 25 ug/kg in mouse by intracranial injection. Does not inhibit Nav1.1/SCN1A, Nav1.2/SCN2A, Nav1.3/SCN3A, Nav1.4/SCN4A, Nav1.5/SCN5A, and Nav1.6/SCN8A voltage-gated sodium channels. Belongs to the long (4 C-C) scorpion toxin superfamily. Sodium channel inhibitor family. Beta subfamily.