Glycolytic enzyme that catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate. (2R)-2-phosphoglycerate = H2O + phosphoenolpyruvate Mg(2+) is required for catalysis and for stabilizing the dimer. Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5. Homodimer. Interacts with PNKD (By similarity). Belongs to the enolase family.