Involved in protein precursor import into chloroplasts. May be part of an intermediate translocation complex acting as a protein-conducting channel at the inner envelope (PubMed:23372012). Plays an important role during embryogenesis and chloroplast biogenesis (PubMed:20738804). Part of the Tic complex. Component of the 1-MD complex, composed of TIC20-I, TIC214, TIC100 and TIC56. Interacts with the translocating preproteins. Hydrolysis of ATP is essential for the formation of this complex (PubMed:23372012). The 1-MD complex interacts with TIC21 (By similarity). A number of isoforms are produced. According to EST sequences. Preferentially expressed in ovules, and moderately expressed in leaves and siliques. Expressed in developing embryo. Severe defects during embryogenesis, producing abnormal embryos and thereby leading to a lethality of young seedlings.