Catalyzes the transfer of the gamma-glutamyl moiety of glutathione (GSH) and other gamma-glutamyl compounds to amino acids and peptides. Major GSH-degrading enzyme, catalyzing the hydrolytic release of L-glutamate from GSH. an alpha-amino acid + an N-terminal (5-L-glutamyl)-[peptide] = 5-L-glutamyl amino acid + N-terminal L-alpha-aminoacyl-[peptide] glutathione + H2O = L-cysteinylglycine + L-glutamate an S-substituted glutathione + H2O = an S-substituted L-cysteinylglycine + L-glutamate Sulfur metabolism; glutathione metabolism. Heterodimer composed of the light and heavy chains. The active site is located in the light chain (By similarity). Induced upon nitrogen starvation. Also induced by non-fermentable carbon sources such as glycerol, acetate and ethanol in a pap1-independent manner. Cleaved by autocatalysis into a large and a small subunit. Belongs to the gamma-glutamyltransferase family.