Catalyzes the NAD(+)-dependent cleavage of saccharopine to L-lysine and 2-oxoglutarate, the final step in the alpha-aminoadipate (AAA) pathway for lysine biosynthesis. H2O + L-saccharopine + NAD(+) = 2-oxoglutarate + H(+) + L-lysine + NADH Inhibited by p-chloromercuribenzoate and iodoacetate by modification of the active site cysteine residue. Inhibited by diethyl pyrocarbonate by modification of histidine residues. Inhibited by pyridoxal 5'-phosphate by modification of an essential lysine residue. Optimum pH is 10 for the forward reaction, and 6.5-7 for the reverse reaction. Amino-acid biosynthesis; L-lysine biosynthesis via AAA pathway; L-lysine from L-alpha-aminoadipate (fungal route): step 3/3. Monomer. Induced by alpha-aminoadipate semialdehyde in a LYS14-dependent manner. Repressed by lysine. Present with 111934 molecules/cell in log phase SD medium. Belongs to the AlaDH/PNT family. PubMed:10077615 shows data confirming the peroxisomal localization of the protein, however this study was later retracted as the images were additionally used in other articles for other proteins.