The exact role played by hoefavidin in the host organism is still obscure. Forms a strong non-covalent complex with biotin and 2-iminobiotin. Is hyperthermostable. Displays a Tm value of 84.9 degrees Celsius, which increases to 96.2 degrees Celsius upon biotin binding. Homodimer (PubMed:26126731). A later study shows that hoefavidin can exist in different homooligomeric states: dimer, tetramer, hexamer and octamer (PubMed:29720692). The X-ray structure of the intact hoefavidin reveals unique crystal packing generated by an octameric cylindrical structure wherein the C-terminal segments of each monomer are introduced into the entrance of the biotin-binding site of an adjacent non-canonical monomer (PubMed:26126731). The specific properties of hoefavidin make it an attractive target for novel biotechnological applications, including drug delivery, nanotechnology, and molecular labeling. Belongs to the avidin/streptavidin family.