Autolysins are involved in some important biological processes such as cell separation, cell-wall turnover, competence for genetic transformation, formation of the flagella - in particular of its basal body - and sporulation. CwlC is able to hydrolyze type A cell walls such as B.subtilis. Its main function is to lyze the mother cell wall peptidoglycan, playing a role during sporulation. Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides. Inhibited by EDTA. Optimum pH is 8.5-9.5. Optimum temperature is 60 degrees Celsius. Is associated with the mother cell wall of sporulating cells. Induced at 6 to 7 hours after sporulation. Contains an N-terminal catalytic domain and two C-terminal tandem repeat sequences that play an important role in peptidoglycan binding. Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family.