Component of the acetyl coenzyme A carboxylase (ACC) complex. First, biotin carboxylase catalyzes the carboxylation of biotin on its carrier protein (BCCP) and then the CO(2) group is transferred by the carboxyltransferase to acetyl-CoA to form malonyl-CoA. Confers resistance to the endogenous polyketide antibiotic thailandamide. Can replace the endogenous gene in S.typhimurium, conferring slow growth and resistance to thailandamide (PubMed:29914944). Can also replace the endogenous gene in E.coli, conferring resistance to thailandamide (PubMed:29975047). acetyl-CoA + N(6)-carboxybiotinyl-L-lysyl-[protein] = malonyl-CoA + N(6)-biotinyl-L-lysyl-[protein] Lipid metabolism; malonyl-CoA biosynthesis; malonyl-CoA from acetyl-CoA: step 1/1. Acetyl-CoA carboxylase is a heterohexamer composed of biotin carboxyl carrier protein (AccB), biotin carboxylase (AccC) and two subunits each of ACCase subunit alpha (AccA) and ACCase subunit beta (AccD). Becomes sensitive to thailandamide antibiotic. Thailandamide is a polyketide that is toxic to human cell lines but also has antibacterial activity on E.coli, S.typhimurium and S.aureus. It probably acts on acetyl-CoA carboxylase in the fatty acid synthesis pathway, which is rarely found to be an antibiotic target. These data suggest it might be a good starting point for engineering of novel antibiotics. Belongs to the AccA family.