RNA 2'-O-methyltransferase involved in the processing of the 34S pre-rRNA to 18S rRNA and in 40S ribosomal subunit formation. (Microbial infection) In case of infection by HIV-1 virus, recruited to HIV-1 RNA and catalyzes 2'-O-methylation of the viral genome, allowing HIV-1 virus to escape the innate immune system (PubMed:30626973). RNA 2'-O-methylation provides a molecular signature for discrimination of self from non-self and is used by HIV-1 to evade innate immune recognition by IFIH1/MDA5 (PubMed:30626973). Mediates methylation of internal residues of HIV-1 RNA, with a strong preference for adenosine (PubMed:30626973). Recruited to HIV-1 RNA via interaction with TARBP2/TRBP (PubMed:30626973). a ribonucleotide in rRNA + S-adenosyl-L-methionine = a 2'-O-methylribonucleotide in rRNA + H(+) + S-adenosyl-L-homocysteine a ribonucleotide in RNA + S-adenosyl-L-methionine = a 2'-O-methylribonucleotide in RNA + H(+) + S-adenosyl-L-homocysteine Interacts with NIP7. (Microbial infection) Interacts with TARBP2/TRBP in case of infection by HIV-1; leading to recruitment to HIV-1 TAR RNA (PubMed:30626973). The complex formed with TARBP2/TRBP is independent of DICER (PubMed:30626973). Citrullinated by PADI4. Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA methyltransferase RlmE family. SPB1 subfamily. Truncated N-terminus.