Involved in resistance to streptothricin, a broad-spectrum antibiotic produced by streptomycetes. Detoxifies streptothricin via acetylation of the beta amino group of the first beta-lysyl moiety of streptothricin. acetyl-CoA + streptothricin D = CoA + H(+) + N(beta)-acetylstreptothricin D acetyl-CoA + streptothricin F = CoA + H(+) + N(beta)-acetylstreptothricin F Homodimer. Deletion mutant is susceptible to streptothricin. Belongs to the acetyltransferase family. GNAT subfamily.