Mechanochemical GTPase component of both plastid and peroxisome division machinery (PubMed:32005784). Required for the last steps of plastid division specifically in mesophyll-cell, when the narrow isthmus breaks, facilitating the separation of the daughter plastids (PubMed:29466386). Necessary for peroxisome activities. Seems to influence stromule (stroma-filled tubular extensions of the plastid envelope membrane) length and frequency (PubMed:29466386). GTP + H2O = GDP + H(+) + phosphate GTPase activity is repressed by PDV2 thus increasing stability at the plastid outer envelope membranes (OEMs) periphery. Forms a homodimer and heterodimers with DRP3A and DRP3B on peroxisomes. Interacts also with FIS1A (but not FIS1B) and PEX11 proteins (PEX11A, PEX11B, PEX11C, PEX11D and PEX11E) on peroxisomes. Interacts with PDV1 and PDV2 (PubMed:32005784). Plastid equatorial positioning (in a discontinuous ring shape) mediated by PDV2 in complex with ARC6 and dissociation from the outer envelope membranes (OEMs) is triggered by PDV1; this localization with OEMs is repressed by phosphatidylinositol 4-phosphate (PI4P) (PubMed:32005784, PubMed:25736058). Dynamic subunit exchange within chloroplastic DRP5B rings with a cytosolic pool during chloroplast membrane constriction in a GTPase activity-dependent manner (PubMed:32005784). Induced by gibberellic acid (GA). Stabilized at the plastid outer envelope membranes (OEMs) in the constriction site when in complex with GTP, but destabilized after conversion of GTP into GDP leading to turnover with a cytosolic pool. Defects in plastid constriction leading to large and dumbbell-shaped chloroplasts in mesophyll-cell only (PubMed:29466386, PubMed:32005784). Sligthly larger division-competent chloroplasts in leaf epidermal pavement cells (PCs) with normal shapes (PubMed:29466386). A few stomatal guard cells (GCs) are missing chlorophyll-bearing plastids (chloroplasts) while accumulating minute chlorophyll-less plastids (PubMed:29466386). Reduced number of large chloroplasts in green tissues. Mostly normal vascular and epidermal chloroplasts and normal plastid size in non green tissues, sometimes exhibiting alteration in stromule length and frequency (e.g. increase in the frequency of stromules in cells of stamen filaments). Presence of highly clustered peroxisomes unable to complete fission and/or enlarged peroxisomes. Impaired peroxisome-related functions, such as photorespiration and fatty acid beta-oxidation. Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA family.