ATP + pyruvate = ADP + H(+) + phosphoenolpyruvate Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5. Homotetramer. Belongs to the pyruvate kinase family. PubMed:1426985 has published some partial sequence, these sequences do not originate from T.acidophilum, rather they seem to be contaminated with human samples.