Involved in the intracellular transport of virions to the host cell surface. Participates also in the formation of actin tails at the plasma membrane to allow efficient actin-based motility and thus cell to cell transmission of viral particles. Recruits host intersectin-1/ITSN1 and activates host CDC42 to drive ARP2/3-mediated actin polymerization. Interacts with host NCK (PubMed:10553910). Interacts with protein A33 (via C-terminus) (PubMed:12634370). Interacts with protein F12 (PubMed:20195521). Interacts (via C-terminus) with host kinesin light chain/KLC1 (PubMed:14963148). Interacts with host intersectin-1/ITSN1 and EPS15 (PubMed:27670116). Found exclusively on the wrapped enveloped virion. Absent in the mature virion (MV) and extracellular enveloped virion (EV). Phosphorylation of Tyr-112 and Tyr-132 of A36 Mechanistically, phosphorylation of Tyr-112 and Tyr-132 of A36 activates the host ARP2-ARP3 complex and leads to actin nucleation. Belongs to the orthopoxvirus A36 protein family.