Involved in mRNA export coupled transcription activation by association with both the TREX-2/AMEX and the SAGA complexes (PubMed:18034162, PubMed:19947544, PubMed:27016737). The SAGA complex is a multiprotein complex that activates transcription by remodeling chromatin and mediating histone acetylation and deubiquitination (PubMed:11438676, PubMed:18206972). Within the SAGA complex, participates in a subcomplex that specifically deubiquitinates histone H2B (PubMed:18206972). The SAGA complex is recruited to specific gene promoters by activators, where it is required for transcription (PubMed:18034162, PubMed:19947544). Required for nuclear receptor-mediated transactivation (PubMed:20714859, PubMed:20048002). Involved in transcription elongation by recruiting the THO complex onto nascent mRNA (PubMed:20048002). The TREX-2/AMEX complex functions in docking export-competent ribonucleoprotein particles (mRNPs) to the nuclear entrance of the nuclear pore complex (nuclear basket) (PubMed:27016737). TREX-2/AMEX participates in mRNA export and accurate chromatin positioning in the nucleus by tethering genes to the nuclear periphery (PubMed:17643381, PubMed:27016737). Recruited to the su(Hw) insulators via its interaction with su(Hw) and participates in the barrier activity of such insulators (PubMed:17643381). In contrast, it does not participate in the enhancer-blocking activity of the su(Hw) insulators (PubMed:17643381). Component of the nuclear pore complex (NPC)-associated TREX-2/AMEX complex (anchoring and mRNA export complex), composed of e(y)2, xmas and PCID2 (PubMed:18034162, PubMed:27016737). Within the TREX-2/ AMEX complex, interactions with xmas is required for localization to the nuclear periphery (PubMed:18034162). Component of the SAGA transcription coactivator-HAT complexes, at least composed of Ada2b, e(y)2, Pcaf/Gcn5, Taf10 and Nipped-A/Trrap (PubMed:18034162, PubMed:19947544). Within the SAGA complex, e(y)2, Sgf11, and not/nonstop form an additional subcomplex of SAGA called the DUB module (deubiquitination module) (By similarity). Component of the THO complex, composed of at least e(y)2, HPR1, THO2, THOC5, THOC6 and THOC7 (PubMed:20714859, PubMed:20048002). Interacts with e(y)1 (PubMed:11438676). Interacts with su(Hw) (via zinc fingers) (PubMed:17643381). Interacts with the nuclear pore complex (NPC) (PubMed:18034162, PubMed:20048002). Interaction between the TREX-2/AMEX complex and the ORC complex is required for ORC localization to mRNPs, and consequently mRNA export (PubMed:27016737, Ref.11). Within the TREX-2/AMEX-ORC complex, interacts with Orc6 and (via N-terminus or C-terminus) with Orc3 (PubMed:27016737, Ref.11). Interacts with the zinc finger protein CG9890 (PubMed:30713769). Localizes to nuclear periphery, in contact with the nuclear pore complex (NPC). Ubiquitous. Detected in embryos (at protein level) (PubMed:27016737, Ref.11). Expressed at all stages of development (PubMed:11438676). Belongs to the ENY2 family.