Catalyzes the efflux of L-lysine (PubMed:8971704, PubMed:1657604, PubMed:1935969, PubMed:27350619). Can also export L-arginine and L-citrulline (PubMed:11429454, PubMed:27350619). The lysEG system prevents bacteriostasis due to elevated L-lysine or L-arginine concentrations that arise during growth in the presence of peptides or in mutants possessing a deregulated biosynthesis pathway (PubMed:11429454). In vitro, can also export D-lysine during biotechnological production of D-amino acids (PubMed:21257776). Transport process is modulated by three forces: the membrane potential, the chemical potential of lysine, and the proton gradient (PubMed:1657604). Strongly inhibited by CCCP and valinomycin (PubMed:1657604). Although six hydrophobic domains were identified based on hydropathy analysis, only five transmembrane spanning helices appear to be present. The additional hydrophobic segment may dip into the membrane or be surface localized. Positively regulated by LysG. Null mutant is unable to excrete L-lysine (PubMed:8971704). Deletion of lysEG decreases the extracellular accumulation of L-lysine, L-arginine and L-citrulline (PubMed:27350619). Belongs to the LysE/ArgO transporter (TC 2.A.75) family. Extended N-terminus.