A non-functional D-aminoacyl-tRNA deacylase (PubMed:4292198, PubMed:24097941, PubMed:25441601). The dtd gene from B.amyloliquefaciens strain A50 confers the ability to grow in the presence of 20 mg/ml D-Tyr and 500 mg/ml D-Asp to B.subtilis, showing it can be used as a selective marker in bacteria that do not have a functional copy of this gene. Spontaneous resistance to D-Tyr is very rare, on the order of 10(-9) (PubMed:25441601). Culture in the presence of D-Leu, D-Met, D-Trp or D-Tyr leads to growth inhibition due to their incorporation into protein, as well as inhibition of biofilm formation (PubMed:24097941). Partially contradictory results are seen in another study where growth occurs in the presence of 20 and 500 mg/ml D-Trp but not in 500 mg/ml D-Asp or D-Ser; whether these are the exact same strains is unknown (PubMed:25441601). Strains in which this gene is restored by mutagenesis are resistant to D-Leu, D-Met, D-Trp and D-Tyr and form biofilm even in the presence of D-amino acids, while maintaining the ability to incorporate at least D-Trp into peptidoglycan (PubMed:24097941). Belongs to the DTD family. Could be the product of a pseudogene. The lack of an initiation codon prevents translation of this gene, in agreement with the observation that D-Tyr-tRNA(Tyr) deacylase activity cannot be detected in some strains of B.subtilis (PubMed:4292198, PubMed:24097941, PubMed:25441601). Mutagenesis to restore the start codon (in strain NCIB 3610, the parent of the fully sequenced 168) confers resistance to D-Tyr (PubMed:24097941).