ATP + pyruvate = ADP + H(+) + phosphoenolpyruvate The activity is regulated by glucose levels. Activated by fructose-1,6-bisphosphate. Optimum pH is 6.0. Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5. Homotetramer. Present with 291000 molecules/cell in log phase SD medium. Belongs to the pyruvate kinase family.