Catalyzes two subsequent steps in gluconeogenesis: the aldol condensation of dihydroxyacetone phosphate (DHAP) and glyceraldehyde-3-phosphate (GA3P) to fructose-1,6-bisphosphate (FBP), and the dephosphorylation of FBP to fructose-6-phosphate (F6P) (By similarity). Can also dephosphorylate, with lower activity, other related substrates including fructose-1-phosphate, fructose-6-phosphate, glucose-1-phosphate, glucose-6-phosphate, glycerol-2-phosphate, phosphoenolpyruvate, 5'-AMP, 6'-ADP and 7'-ATP (PubMed:21221938). beta-D-fructose 1,6-bisphosphate = D-glyceraldehyde 3-phosphate + dihydroxyacetone phosphate beta-D-fructose 1,6-bisphosphate + H2O = beta-D-fructose 6-phosphate + phosphate FBPase activity is inhibited by Ca(2+), ATP, ADP and phosphoenolpyruvate. kcat is 14.84 sec(-1) for the FBPase activity (at 95 degrees Celsius). Optimum pH is 8.0. Optimum temperature is 95 degrees Celsius. Carbohydrate biosynthesis; gluconeogenesis. Homooctamer; dimer of tetramers. Consists of a single catalytic domain, but remodels its active-site architecture via a large structural change to exhibit dual activities. Belongs to the FBP aldolase/phosphatase family.