T1-TrpRS has aminoacylation activity while T2-TrpRS lacks it. T1-TrpRS and T2-TrpRS possess angiostatic activity. T2-TrpRS inhibits fluid shear stress-activated responses of endothelial cells. Regulates ERK, Akt, and eNOS activation pathways that are associated with angiogenesis, cytoskeletal reorganization and shear stress-responsive gene expression (By similarity). ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + H(+) + L-tryptophanyl-tRNA(Trp) Homodimer (By similarity). Interacts with oxidized form of GAPDH (By similarity). Proteolytic cleavage generates 2 forms; T1-TrpRS and T2-TrpRS. Belongs to the class-I aminoacyl-tRNA synthetase family. Truncated N-terminus.