NADP-dependent (R)-specific alcohol dehydrogenase (ADH) with a broad substrate specificity, able to catalyze in vitro the stereoselective reduction of several aliphatic and aromatic ketones as well as beta-keto esters to the corresponding enantiomerically pure alcohols. a secondary alcohol + NADP(+) = a ketone + H(+) + NADPH acetophenone + H(+) + NADPH = (R)-1-phenylethanol + NADP(+) 2,5-hexanedione + 2 H(+) + 2 NADPH = (2R,5R)-hexanediol + 2 NADP(+) ethyl 3-oxobutanoate + H(+) + NADPH = ethyl (R)-3-hydroxybutanoate + NADP(+) 2-octanone + H(+) + NADPH = (2R)-octan-2-ol + NADP(+) Optimum pH is 7.0 with acetophenone as substrate. Optimum temperature is 50 degrees Celsius with acetophenone as substrate. Homotetramer. Because of its broad substrate specificity and its ability to produce chiral alcohols with high enantiomeric excesses, this protein from L.kefiri is a very interesting enzyme for preparative applications in the synthesis of pharmaceuticals and agrochemicals. Belongs to the short-chain dehydrogenases/reductases (SDR) family.