Formation of pseudouridine at positions 27 and 28 in the anticodon stem and loop of transfer RNAs; at positions 34 and 36 of intron-containing precursor tRNA(Ile) and at position 35 in the intron-containing tRNA(Tyr). Catalyzes pseudouridylation at position 44 in U2 snRNA. Also catalyzes pseudouridylation of mRNAs. a uridine in tRNA = a pseudouridine in tRNA uridine in snRNA = pseudouridine in snRNA a uridine in mRNA = a pseudouridine in mRNA Binds 1 zinc ion per subunit. Belongs to the tRNA pseudouridine synthase TruA family.