Molecular chaperone which mediates the proper assembly and functional expression of the nicotinic acetylcholine receptors (nAChRs) throughout the brain (PubMed:26875622). Essential for the proper folding, assembly, function and surface trafficking of alpha-7 (CHRNA7), alpha-4-beta-2, alpha-3-beta-2 and alpha-3-beta-4 receptors (PubMed:26875622). Stably associates with ribophorin-1 (RPN1) and ribophorin-2 (RPN2) (components of the oligosaccharyl transferase (OST) complex) and with calnexin (CANX), both of which are critical for NACHO-mediated effects on CHRNA7 assembly and function (By similarity). Facilitates the proper folding and assembly of alpha-6-beta-2 and alpha-6-beta-2-beta-3 receptors and acts at early stages of the nAChRs subunit assembly (PubMed:26875622). Promotes the expression of the alpha-4(2):beta-2(3) stoichiometric form over the alpha-4(3):beta-2(2) form (By similarity). May interact with NGFR (PubMed:20685870). Interacts with RPN1, RPN2 and CANX (By similarity). Shedding may lead to a soluble peptide. Present in adrenal zona glomerusa but not in adrenal medulla (at protein level) (PubMed:20685870). Expressed in the hippocampus (at protein level) (PubMed:26875622). Levels decrease during early postnatal development after which it remains stable into adulthood and this decrease occurs to a greater degree in the frontal cortex compared to the hippocampus (at protein level). By sodium depletion and angiotensin II in adrenal zona glomerusa (at protein level). Knockdown in cultured hippocampal neurons shows a complete disruption of the assembly and function of the neuronal acetylcholine receptor subunit alpha-7 (CHRNA7). Belongs to the DoxX family.