Cytoskeletal protein required for organization of normal actin cytoskeleton. Roles in establishing cell morphology, motility, cell adhesion and cell-extracellular matrix interactions in a variety of cell types. May function as a scaffolding molecule with the potential to influence both actin polymerization and the assembly of existing actin filaments into higher-order arrays. Binds to proteins that bind to either monomeric or filamentous actin. Localizes at sites where active actin remodeling takes place, such as lamellipodia and membrane ruffles. Different isoforms may have functional differences. Plays a role in neurite outgrowth and in the establishment of polarity during neuronal morphogenesis. Participates in the acquisition of the reactive astrocyte morphology. Interacts with EPS8 (By similarity). Interacts with LASP1 (By similarity). Interacts with VASP (By similarity). Interacts with ACTN (By similarity). Interacts with SORBS2 (By similarity). Interacts with PFN1 (By similarity). Interacts with LPP (By similarity). Interacts with SPIN90 (By similarity). Interacts with SRC (By similarity). Interacts with EZR (By similarity). Interacts with RAI14 (PubMed:23565266). Localizes to stress fibers (PubMed:16868024, PubMed:11553711, PubMed:16125169, PubMed:22659164). Localizes to Z lines (PubMed:16868024, PubMed:11553711, PubMed:16125169). Preferentially expressed in the excitatory presynaptic terminals (PubMed:11438925). In adult central nervous system is detected in the brain and spinal cord, specially in the olfactory bulb, cerebral and cerebellar cortices, hippocampus, amygdala, superior colluculus, and superficial laminae of the spinal dorsal horn. Early expressed in the axonal compartment in developing neurons, and persists in this polarized distribution in the adult brain. Up-regulated rapidly and persistently in astrocytes in response to injury. Phosphorylated predominantly on serines and, to a lesser extent, on tyrosines. Phosphorylation at Ser-395 by PKB/AKT1 modulates cytoskeletal organization and cell motility (By similarity). Belongs to the myotilin/palladin family.