Catalyzes the conversion of ureidosuccinic acid (USA) to dihydroorotate, the third step of the de novo pyrimidine biosynthetic pathway. (S)-dihydroorotate + H2O = H(+) + N-carbamoyl-L-aspartate Binds 2 Zn(2+) ions per subunit. Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 3/3. By N-carbamoyl-L-aspartate. Present with 12700 molecules/cell in log phase SD medium. Belongs to the metallo-dependent hydrolases superfamily. DHOase family. Class II DHOase subfamily.