Probably involved in fatty acid metabolism. Binds to fatty acyl-CoAs of varying carbon chain lengths, with the highest binding affinity for palmitoyl-CoA (C16:0). In vitro, alters the cell wall lipid profile and protects mycobacteria from acidic, oxidative and antibiotic stress. May play a significant role in host-pathogen interaction. Homodimer. Consists of a large Rossmann fold domain and a small domain, which are connected by a flexible linker. Belongs to the CoA-transferase III family.