Regulates ATP-dependent protein translocation into the mitochondrial matrix. Inhibits DNAJC19 stimulation of HSPA9/Mortalin ATPase activity. Probable component of the PAM complex at least composed of a mitochondrial HSP70 protein, GRPEL1 or GRPEL2, TIMM44, TIMM16/PAM16 and TIMM14/DNAJC19 (By similarity). Interacts with DNAJC19. Directly interacts with DNAJC15; this interaction counteracts DNAJC15-dependent stimulation of HSPA9 ATPase activity. Associates with the TIM23 complex. Associates with the TIM23 complex (By similarity). Ubiquitously expressed. By CSF2/GM-CSF. The J-like region, although related to the J domain does not have co-chaperone activity. The disease is caused by variants affecting the gene represented in this entry. Belongs to the TIM16/PAM16 family.