Catalyzes the formation of pyruvate in the last step of glycolysis, it is irreversible under physiological conditions. The reaction is critical for the control of metabolic flux in the second part of glycolysis. ATP + pyruvate = ADP + H(+) + phosphoenolpyruvate Allosterically activated by AMP and by several sugar phosphates. Belongs to type II PK. Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5. Homotetramer. Belongs to the pyruvate kinase family.