NADPH-dependent oxidoreductase which catalyzes the reduction of a variety of compounds bearing carbonyl groups including ketosteroids, alpha-dicarbonyl compounds, aldehydes, aromatic ketones and quinones (PubMed:18571493, PubMed:19056333). Reduces 3-ketosteroids and benzil into 3beta-hydroxysteroids and R-benzoin, respectively, in contrast to the stereoselectivity of non-primate DHRS4s which produce 3alpha-hydroxysteroids and S-benzoin (PubMed:19056333). Diplays low activity toward all-trans-retinal and no activity toward 9-cis-retinal as compared to non-primate mammals (PubMed:18571493, PubMed:19056333). In the reverse reaction, catalyze the NAD-dependent oxidation of 3beta-hydroxysteroids and alcohol, but with much lower efficiency (PubMed:18571493, PubMed:19056333). Involved in the metabolism of 3beta-hydroxysteroids, isatin and xenobiotic carbonyl compounds (PubMed:18571493, PubMed:19056333). No detected catalytic activity in vitro, possibly due to the lack of catalytic site. NADPH-dependent oxidoreductase which catalyzes the reduction of a variety of compounds bearing carbonyl groups including ketosteroids, alpha-dicarbonyl compounds, aldehydes, aromatic ketones and quinones. Involved in the metabolism of 3beta-hydroxysteroids, isatin and xenobiotic carbonyl compounds. Has a higher catalytic activity for xenobiotic alpha-dicarbonyl compounds, sucha as benzil, than isoform 1 and is involved in benzil detoxification. a secondary alcohol + NADP(+) = a ketone + H(+) + NADPH a secondary alcohol + NADP(+) = a ketone + H(+) + NADPH 3beta-hydroxy-5beta-pregnane-20-one + NADP(+) = 5beta-pregnan-3,20-dione + H(+) + NADPH 3beta-hydroxy-5beta-pregnane-20-one + NADP(+) = 5beta-pregnan-3,20-dione + H(+) + NADPH 5beta-dihydrotestosterone + H(+) + NADPH = 5beta-androstane-3beta,17beta-diol + NADP(+) 5beta-androstane-3,17-dione + H(+) + NADPH = 3beta-hydroxy-5beta-androstane-17-one + NADP(+) H(+) + isatin + NADPH = 3-hydroxyindolin-2-one + NADP(+) H(+) + isatin + NADPH = 3-hydroxyindolin-2-one + NADP(+) lithocholate + NADP(+) = 3-oxo-5beta-cholan-24-oate + H(+) + NADPH 3-oxo-5beta-cholan-24-oate + H(+) + NADPH = isolithocholate + NADP(+) Inhibited by flavonoids (quercetin and genistein), cetylpyridium chloride, phenylhexane and valproic acid. Low inhibition is observed with fatty acids (myristic acid and lauric acid). No significant inhibition is observed with barbital, dicumarol, indomethacin, metyrapone, ethacrynic acid, disulfiram, hexestrol and benzodiazepines (diazepam and nitrazepam). kcat is 3.4 min(-1) with 5beta-Pregnane-3,20-dione as substrate (at pH7.4) (PubMed:23128527). kcat is 11.8 min(-1) with 5beta-Androstan-17beta-ol-3-one as substrate (at pH7.4) (PubMed:23128527). kcat is 475 min(-1) with Isatin as substrate (at pH6) (PubMed:23128527). kcat is 3600 min(-1) with Benzil as substrate (at pH6) (PubMed:23128527). kcat is 2570 min(-1) with 1-Phenylisatin as substrate (at pH6) (PubMed:23128527). kcat is 737 min(-1) with 9,10-Phenanthrenequinone as substrate (at pH7.4) (PubMed:23128527). kcat is 27.1 min(-1) with Menadione as substrate (at pH6) (PubMed:23128527). kcat is 3.8 min(-1) with 5beta-Pregnane-3,20-dione as substrate (at pH7.4) (PubMed:18571493). kcat is 5 min(-1) with 5beta-Androstan-17beta-ol-3-one as substrate (at pH7.4) (PubMed:18571493). kcat is 4.6 min(-1) with 5beta-Androstane-3,17-dione as substrate (at pH7.4) (PubMed:18571493). kcat is 4.2 min(-1) with 5b-Pregnan-20alpha-ol-3-one as substrate (at pH7.4) (PubMed:18571493). kcat is 5.7 min(-1) with 5alpha-Dihydrotestosterone as substrate (at pH7.4) (PubMed:18571493). kcat 5.8 is min(-1) with 5alpha-Androstane-3,17-dione as substrate (at pH7.4) (PubMed:18571493). kcat is 0.41 min(-1) with Testosterone as substrate (at pH7.4) (PubMed:18571493). kcat is 0.02 min(-1) with 5alpha-Pregnane-3,20-dione as substrate (at pH7.4) (PubMed:18571493). kcat is 2.6 min(-1) with Dehydrolithocholic acid as substrate (at pH7.4) (PubMed:18571493). kcat is 3.2 min(-1) with all-trans-Retinal as substrate (at pH7.4) (PubMed:18571493). kcat is 1900 min(-1) with Isatin as substrate (at pH6) (PubMed:18571493). kcat is 2000 min(-1) with Dimethyl-2-oxoglutarate as substrate (at pH6) (PubMed:18571493). kcat is 2000 min(-1) with 4-Hexanoylpyridine as substrate (at pH6) (PubMed:18571493). kcat is 160 min(-1) with Hexanophenone as substrate (at pH6) (PubMed:18571493). kcat is 120 min(-1) with Valerophenone as substrate (at pH6) (PubMed:18571493). kcat is 51 min(-1) with n-Butyrophenone as substrate (at pH6) (PubMed:18571493). kcat is 120 min(-1) with 4-Benzoylpyridine as substrate (at pH6) (PubMed:18571493). kcat is 980 min(-1) with 3,4-Hexanedione as substrate (at pH6) (PubMed:18571493). kcat is 2410 min(-1) with 2,3-Heptanedione as substrate (at pH6) (PubMed:18571493). kcat is 1900 min(-1) with Benzil as substrate (at pH6) (PubMed:18571493). kcat is 220 min(-1) with 4,4'-Dimethylbenzil as substrate (at pH6) (PubMed:18571493). kcat is 1600 min(-1) with 2,3-Pentanedione as substrate (at pH6) (PubMed:18571493). kcat is 1700 min(-1) with Diacetyl as substrate (at pH6) (PubMed:18571493). kcat is 1000 min(-1) with 4-Nitrobenzaldehyde as substrate (at pH6) (PubMed:18571493). kcat is 280 min(-1) with Pyridine-4-aldehyde as substrate (at pH6) (PubMed:18571493). kcat is 2500 min(-1) with 1-Phenylisatin as substrate (at pH6) (PubMed:18571493). kcat is 710 min(-1) with 9,10-Phenanthrenequinone as substrate (at pH6) (PubMed:18571493). kcat is 39 min(-1) with Menadione as substrate (at pH6) (PubMed:18571493). kcat is 120 min(-1) with 6-tert-butyl-2,3-epoxy-5-cyclohexene-1,4-dione as substrate (at pH6) (PubMed:18571493). kcat is 53 min(-1) with 1,4-Naphthoquinone as substrate (at pH6) (PubMed:18571493). kcat is 2.5 min(-1) with 5beta-Pregnan-3beta-ol-20-one as substrate (at pH6) (PubMed:18571493). kcat is 0.42 min(-1) with 5beta-Pregnan-3beta-ol-20-one as substrate (at pH6) (PubMed:19056333). kcat is 1 min(-1) with 5beta-Androstane-3beta,17beta-diol as substrate (at pH6) (PubMed:18571493). kcat is 0.24 min(-1) with 5beta-Androstane-3beta,17beta-diol as substrate (at pH6) (PubMed:19056333). kcat is 1 min(-1) with 5beta-Androstan-3beta-ol-17-one as substrate (at pH7.4) (PubMed:18571493). kcat is 0.84 min(-1) with 5beta-Pregnane-3beta,20alpha-diol as substrate (at pH7.4) (PubMed:18571493). kcat is 0.86 min(-1) with 5alpha-Androstane-3beta,17beta-diol as substrate (at pH7.4) (PubMed:18571493). kcat is 0.43 min(-1) with 5alpha-Pregnane-3beta-ol-20-one as substrate (at pH7.4) (PubMed:18571493). kcat is 0.21 min(-1) with Isolithocholic acid as substrate (at pH7.4) (PubMed:18571493). kcat is 0.21 min(-1) with Isolithocholic acid as substrate (at pH7.4) (PubMed:19056333). kcat is 0.02 min(-1) with 5alpha-Androstan-3beta-ol-17-one as substrate (at pH7.4) (PubMed:18571493). kcat is 4.5 min(-1) with S-Phenyl-1-butanol as substrate (at pH7.4) (PubMed:18571493). kcat is 15 min(-1) with S-1-Tetralol as substrate (at pH7.4) (PubMed:18571493). kcat is 11 min(-1) with S-1-Indanol as substrate (at pH7.4) (PubMed:18571493). kcat is 0.2 min(-1) with all-trans-Retinol as substrate (at pH7.4) (PubMed:18571493). Homotetramer. Isoform 4 is not peroxisomal. Predominantly expressed in normal cervix (at protein level). Expressed in some neoplastic cervical tissues, but not in normal cervix (at protein level). Expressed in a few neoplastic cervical tissues. Expressed in a few neoplastic cervical tissues. High expression in liver. Induced by PPARA ligands clofibrate and Wy14,643. The C-terminus peroxisomal targeting signal tripeptide is important for peroxisomal import. Once in the peroxisome, it is involved in intersubunit interactions. Three specific residues, Ser-176, Phe-179 and Thr-195 are conserved between primates whereas the respective residues are phenylalanine, leucine, and asparagine in the other mammal enzymes (PubMed:18571493, PubMed:19056333). The two residues at positions 176 and 179 are molecular determinants responsible for the stereoselective reduction of 3-ketosteroids and benzil (PubMed:19056333). The presence of an asparagine at position 195 is important for the maintenance of the quaternary structure and stability at cold temperature (PubMed:18571493, PubMed:19056333). The absence of an asparagine at position 195 destabilizes the quaternary structure, thereby affecting catalytic efficiency toward some substrates and decreasing stability at cold temperature (PubMed:18571493, PubMed:19056333). Primate DHRS4s display different stereoselectivity and catalytic efficiency in the oxidoreduction of some substrates as compared to other mammal DHRS4s due to a difference in conserved amino acid residues (PubMed:18571493, PubMed:19056333). Three homologous proteins DHRS4, DHRS4L1, and DHRS4L2 are derived from gene duplication of DHRS4, and the gene cluster is arranged in tandem in chromosome 14 (PubMed:27323117). Belongs to the short-chain dehydrogenases/reductases (SDR) family. Truncated N-terminus. Truncated N-terminus. Truncated N-terminus. Truncated N-terminus.