Cobalamin (vitamin B12) cytosolic chaperone that catalyzes the reductive decyanation of cyanocob(III)alamin (cyanocobalamin, CNCbl) to yield cob(II)alamin and cyanide, using FAD or FMN as cofactors and NADPH as cosubstrate. Cyanocobalamin constitutes the inactive form of vitamin B12 introduced from the diet, and is converted into the active cofactors methylcobalamin (MeCbl) involved in methionine biosynthesis, and 5'-deoxyadenosylcobalamin (AdoCbl) involved in the TCA cycle. Forms a complex with the lysosomal transporter ABCD4 and its chaperone LMBRD1, to transport cobalamin across the lysosomal membrane into the cytosol. The processing of cobalamin in the cytosol occurs in a multiprotein complex composed of at least MMACHC, MMADHC, MTRR (methionine synthase reductase) and MTR (methionine synthase) which may contribute to shuttle safely and efficiently cobalamin towards MTR in order to produce methionine. Also acts as a glutathione transferase by catalyzing the dealkylation of the alkylcob(III)alamins MeCbl and AdoCbl, using the thiolate of glutathione for nucleophilic displacement to generate cob(I)alamin and the corresponding glutathione thioether. The conversion of incoming MeCbl or AdoCbl into a common intermediate cob(I)alamin is necessary to meet the cellular needs for both cofactors. Cysteine and homocysteine cannot substitute for glutathione in this reaction. 2 cob(II)alamin-[cyanocobalamin reductase] + 2 hydrogen cyanide + NADP(+) = 2 apo-[cyanocobalamin reductase] + 2 cyanocob(III)alamin + H(+) + NADPH an R-cob(III)alamin + apo-[alkylcobalamin reductase] + glutathione = an S-substituted glutathione + cob(I)alamin-[alkylcobalamin reductase] + H(+) apo-[alkylcobalamin reductase] + glutathione + methylcob(III)alamin = cob(I)alamin-[alkylcobalamin reductase] + H(+) + S-methyl glutathione adenosylcob(III)alamin + apo-[alkylcobalamin reductase] + glutathione = cob(I)alamin-[alkylcobalamin reductase] + H(+) + S-adenosylglutathione Can utilize both FAD and FMN. Monomer in the absence of bound substrate. Homodimer; dimerization is triggered by binding to FMN or adenosylcobalamin. Interacts with LMBRD1 and ABCD4; the interaction ensures the transport of cobalamin from the lysosome to the cytoplasm. Forms a multiprotein complex with MMADHC, MTR and MTRR; the interaction with MTR could modulate MMACHC-dependent processing of cobalamin. Heterodimer with MMADHC; the interaction might play a role in the regulation of the balance between AdoCbl and MeCbl synthesis. Detected in liver and kidney (at protein level) (PubMed:21697092). Detected in embryos (PubMed:24889031). Complete embryonic lethality. All die before 3.5 dpc. Belongs to the MMACHC family.