Essential for life in oxygen, but nonessential under anaerobic conditions. Required for biogenesis of the large ribosomal subunit and initiation of translation in oxygen (PubMed:23318452). The complex LTO1:YAE1 functions as a target specific adapter that recruits apo-RLI1 to the cytosolic iron-sulfur protein assembly (CIA) complex machinery (PubMed:26182403). Forms a complex with YAE1; the complex bridges the interaction between the CIA complex and RLI1 (PubMed:23318452). Associates with the CIA complex (via its C-terminal tryptophan) (PubMed:26182403). Present with 784 molecules/cell in log phase SD medium. Belongs to the LTO1 family. Extended N-terminus. Extended N-terminus. Extended N-terminus.