Alpha-conotoxins act on postsynaptic membranes, they bind to the nicotinic acetylcholine receptors (nAChR) and thus inhibit them (PubMed:31255696). This toxin potently and selectively inhibits human and rat alpha-6-beta-4/CHRNA6-CHRNB4 nAChR (IC(50)=12 nM on rat nAChR) (PubMed:31255696). It exhibits rapid binding and unbinding at this receptor (PubMed:31255696). It also shows activity on rat alpha-6-beta-4/CHRNA6-CHRNB4 (IC(50)=12 nM), human alpha-6/alpha-3-beta-4 (CHRNA6/CHRNA3-CHRNB4) (IC(50)=5.3 nM), rat alpha-6/alpha-3-beta-4 (CHRNA6/CHRNA3-CHRNB4) (IC(50)=18 nM), rat alpha-3-beta-4/CHRNA3-CHRNB4 (IC(50)=320 nM), and rat alpha-6/alpha-3-beta-2-beta-3 (CHRNA6/CHRNA3-CHRNB2-CHRNB3) (IC(50)=4 uM) (PubMed:31255696). Expressed by the venom duct. The cysteine framework is I (CC-C-C). Alpha4/6 pattern. Has no effect on human alpha-1-beta-1-delta-epsilon/CHRNA1-CHRNB1-CHRNE-CHRND, rat alpha-2-beta-2/CHRNA2-CHRNB2, rat alpha-2 beta-4/CHRNA2-CHRNB4, rat alpha-3 beta-2/CHRNA3-CHRNB2, rat alpha-4 beta-2/CHRNA4-CHRNB2, rat alpha-4 beta-4/CHRNA4-CHRNB4, rat alpha-7/CHRNA7, rat alpha-9-alpha-10/CHRNA9-CHRNA10, human alpha-3-beta-4/CHRNA3-CHRNB4, human alpha-6/alpha-3-beta-2-beta-3 (CHRNA6/CHRNA3 AND CHRNB2 AND CHRNB3), human alpha-4-beta-2/CHRNA4-CHRNB2. Belongs to the conotoxin A superfamily. Extended N-terminus.