Hydrolyzes triglycerides, with a preference for substrates with short-chain lengths (C4 to C8). Has the highest activity with tributyrin (C4), followed by tricaproin (C6) and tricaprylin (C8). Can also hydrolyze vinylacetate (C2) and triolein (C18), but with lower efficiency. Has no activity with tripalmitin (C16). a triacylglycerol + H2O = a diacylglycerol + a fatty acid + H(+) Optimum pH is between 7-9. Optimum temperature is 40-70 degrees Celsius. Monomer. In addition to their capacity to hydrolyze a wide variety of natural and non-natural substrates, lipases can also catalyze the formation of ester bonds between carboxylic acids and alcohols, making them attractive biocatalysts that can contribute to the synthesis of pharmaceuticals and other fine chemicals. Sold under the name lipase novozym 735. Belongs to the AB hydrolase superfamily. Lipase family. Extended N-terminus.