Resistance to antibiotics containing the 2-deoxy-streptamine ring including dibekacin, gentamicin, kanamycin, sisomicin, tobramycin, neomycin and to a lesser extent netilmicin. a 2-deoxystreptamine antibiotic + acetyl-CoA = an N(3')-acetyl-2-deoxystreptamine antibiotic + CoA + H(+) Belongs to the antibiotic N-acetyltransferase family.