During mitosis, may play a role in the metaphase-to-anaphase transition. Interacts with PICALM; this interaction may target PICALM to the nucleus. During mitosis, associates with HDAC2 and MTA2 subunits of the chromatin-remodeling NuRD complex; this association is strongest at prometaphase and decreases as the cell progresses through metaphase and anaphase. Partially localizes to the nucleolus. Mainly expressed in thymus and ovary. Expressed in all T-cell subpopulations isolated from the thymus, macrophages, pro-erythrocytes, granulocytes, mast cells and progenitor cells. Widely expressed at 9.5 dpc, including high levels in the neural tube, somites, the posterior region of the midbrain, olfactory placode and the branchial arches. At 10.5 dpc, reduction of the widespread expression and stronger expression in the neural tube, branchial arches, developing limbs, telencephalon, nasal process, lense vesicle, anterior and posterior regions of the mid- and hindbrain. From 11.5 dpc on, strongly expressed in the genital tubercle and hair and vibrissae follicles. From 12.5 dpc onwards, expression decreases, with a complete lack of expression in the cephalic region and the neural tube at 14.5 dpc. Strongly expressed during limb development, with higher levels in hindlimbs compared to forelimbs and expression slightly more marked in the posterior region of the limb buds. At 11.5 and 12.5 dpc, detected at the distal domain and the underlying mesenchyme, but not in the apical ectodermal ridge. Distally, becomes confined to the digits at 13.5 and 14.5 dpc. The N-terminal destruction box 2 (D-box 2) is required for APC/C ubiquitination and proteasomal degradation. Ubiquitinated by the anaphase-promoting complex/cyclosome (APC/C) complex in the presence of FZR1, leading to its degradation by the proteasome during mitotic exit. However, degradation is not essential for normal mitotic progression within a single cell cycle (By similarity).