alpha,alpha-trehalose + H2O = alpha-D-glucose + beta-D-glucose Inhibited by sodium, potassium and ammonium ions, and by TEMED. Optimum pH is 6.7 when incubated for 5 minutes at 35 degrees Celsius. Active from pH 4.5 to 12.5 when incubated for 5 minutes at 35 degrees Celsius. Optimum pH is 7.0 when incubated for 24 hours at 4 degrees Celsius. Active from pH 5.0 to 10.5 when incubated for 24 hours at 4 degrees Celsius. Stable up to 40 degrees Celsius, but activity is lost after 15 minutes incubation at 60 degrees Celsius. Monomer. Glycosylated; contains 3.1% carbohydrates. Belongs to the glycosyl hydrolase 37 family.