Catalyzes the phosphorylation of dihydroxyacetone. ATP + dihydroxyacetone = ADP + dihydroxyacetone phosphate + H(+) Divalent metal cations, Mg(2+) or Ca(2+). Polyol metabolism; glycerol fermentation; glycerone phosphate from glycerol (oxidative route): step 2/2. Homodimer. The C-terminal domain consists of a eight-helix alpha barrel. The eight helices form a pocket that includes a tightly bound phospholipid. The cellular function of this lipid is unknown; it has no significant effect on enzyme activity.