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What is the difference between the use of Tris-base and Tris-HCL in the preparation of gels for SDS-PAGE other than the volume of NaOH or HCL needed for the pH adjust?
Besides the pH, are there any other factors that could be affected by using Tris-base or Tris-HCL in gel preparation?
Yes, apart from pH, the use of Tris-base or Tris-HCL could also affect the conductivity of the buffer solution, which can impact the resolution and separation of proteins on the gel. Additionally, the choice between the two could also affect the buffering capacity of the gel, which could impact the speed of the electrophoresis process.