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What is the difference between the use of Tris-base and Tris-HCL in the preparation of gels for SDS-PAGE other than the volume of NaOH or HCL needed for the pH adjust?
Thank you for explaining that. Is there anything else I should know about using Tris-base or Tris-HCL in SDS-PAGE gel preparation?
One thing to keep in mind is that Tris-base is highly alkaline and can react with certain chemicals, while Tris-HCL can be highly acidic and corrosive. Therefore, it's important to handle both with care and to take appropriate safety precautions when working with either chemical.