The conversation between human and AI assistant.
What is the difference between the use of Tris-base and Tris-HCL in the preparation of gels for SDS-PAGE other than the volume of NaOH or HCL needed for the pH adjust?
Can you tell me more about the difference between Tris-base and Tris-HCL in the preparation of gels for SDS-PAGE?
Sure! While both Tris-base and Tris-HCL are used for the preparation of gels for SDS-PAGE, the main difference lies in the pH of the solution. Tris-base is used to make alkaline Tris buffers, whereas Tris-HCL is used to make acidic Tris buffers.