The conversation between human and AI assistant.
What is the difference between the use of Tris-base and Tris-HCL in the preparation of gels for SDS-PAGE other than the volume of NaOH or HCL needed for the pH adjust?
How does this difference affect the gel preparation process?
When making a gel for SDS-PAGE, the pH of the sample buffer will determine the charge of the protein molecules being separated. Therefore, the choice between Tris-base and Tris-HCL will affect the pH of the sample buffer and thus the charge of the protein molecules.