Topic: cloning
Stance: PRO
Aspect: resources
In addition to being able to create clones from any source material ( including your own DNA ) , this kit also includes all necessary tools you need to successfully perform PCR reactions : 1 - High-quality miniprep gel containing 5 ' -proofed Taq polymerase ; 2 - A high-efficiency TOPO TA Cloning vector ; 3 - Restriction enzyme ; 4 - Safe temperature control ; 5 - Buffer solution ; 6 - Purification step ; 7 - Incubation phase ; 8 - Final Step If you are working with an existing library or collection of cDNAs , then there 's no reason why you should n't use the same protocol as described in Example 9 .