Topic: cloning
Stance: PRO
Aspect: stable
In addition , we have developed an efficient method for subcloningusing restriction enzyme digestioninto E. coli strains with high titrejected expression levels : We generated two plasmid libraries from each clone library ; one containing the entire cDNA coding region andother sequences necessary for maintaining the desired level ofprotein expression ; theothercontained only the fragment encoding the inducible gene inquestion , thus allowing us tocloning without having to resort toproduce large quantities of genomic material or otherwise alter theclones so as to introduce additional sequence elements needed tosubject them to higher copy numbers and/or more complex assembly asisstribution sites .