Since the TSR's closure, the right-of-way has become popular for strolling and hiking. From 1970, the Guelph Hiking Trail Club was formed to establish and maintain a formal trail on it between the Bruce Trail at Limehouse and Guelph. A small part of the Guelph line's right-of-way is used by the Halton County Radial Railway museum. The areas where tracks were once located include the walking trails along the south bank of the Eramosa River, the Smith Property loop in Puslinch and the Halton County Radial Railway site on Guelph Line, Milton.