After County Road 464A (North Magnolia Avenue) terminates at the southeast corner of the intersection of US 301-441 and Northwest 28th Street, another county alternate which was a former section of US 441-SR 25 begins on the opposite side. This road is CR 25A (Northwest Gainesville Road), which is also the decommissioned U.S. Route 441 Alternate, and runs northwest through towns such as Zuber, Lowell, and Reddick. East of Zuber, the road intersects SR 326, which originally had a short concurrency, but now directly crosses US 301-441. South of the intersection with CR 329 is an interchange with U.S. Route 441 between Sparr and Lowell, where the US 301-441 concurrency ends. US 301 moves northeast towards Waldo, Starke, and Jacksonville taking SR 200 with it, while US 441 moves northwest.