At the time "Family Guy" was being developed, Borstein was working in the sketch comedy, "MADtv". She was asked to audition by a member of the "MADtv" staff who was helping MacFarlane develop the show. She had not met MacFarlane or seen any artwork and said it was "really sight unseen". At the time, she was doing a stage show in Los Angeles, in which she played a redhead mother, whose voice she had based on one of her cousins from Long Island, New York. She took the voice of the character to the set and use it for Lois. The voice was originally slower, when MacFarlane heard it, he asked her to make it faster and higher. Borstein has noted that the voice of Lois has been changing from the slower original voice to the quicker up tempo voice of the present episodes.