I-490 continues to run in the former bed until exit 21, where I-490 connects to NY 590 and I-590 at an interchange known locally as the Can of Worms. At this point, the former Erie Canal route (now part of I-590) curves southward while I-490 continues eastward into the eastern suburbs of Rochester. Between exits 21 and 24, I-490 parallels the CSX Transportation-owned Rochester Subdivision rail line, intersecting NY 441, a four-lane divided highway in the process. South of exit 25, I-490 traverses the southeastern suburbs of Rochester, passing close to East Rochester, Pittsford and Bushnell's Basin and closely paralleling NY 96 on its way toward the Ontario County line. The freeway meets NY 96 twice in Perinton and a third time in the Ontario County town of Victor, where I-490 passes along the western fringe of Eastview Mall. I-490 ends a short distance southeast of the third NY 96 interchange at Thruway exit 45.