Despite the operational and maintenance obstacles, construction of the pump enabled Boyle to conduct a great many experiments on the properties of air, which he later detailed in his "New Experiments Physico-Mechanicall, Touching the Spring of the Air, and its Effects, (Made, for the Most Part, in a New Pneumatical Engine)". In the book, he described in great detail 43 experiments he conducted, on occasion assisted by Hooke, on the effect of air on various phenomena. Boyle tested the effects of "rarified" air on combustion, magnetism, sound, and barometers, and examined the effects of increased air pressure on various substances. He listed two experiments on living creatures: "Experiment 40", which tested the ability of insects to fly under reduced air pressure, and the dramatic "Experiment 41," which demonstrated the reliance of living creatures on air for their survival. In this attempt to discover something "about the account upon which Respiration is so necessary to the Animals, that Nature hath furnish'd with Lungs", Boyle conducted numerous trials during which he placed a large variety of different creatures, including birds, mice, eels, snails and flies, in the vessel of the pump and studied their reactions as the air was removed. Here, he describes an injured lark: