The athletic teams of Brentsville District High School (during the regular season) compete in the AA Evergreen District and Region II for which the governing body is the Virginia High School League. Brentsville is, as of 2015, one of the smallest schools to compete in Group 3A athletics. For post-season purposes, Brentsville is assigned to Conference 28 as it is a Group 3A school in the eastern region. Before the 2004 academic year, Brentsville competed in the A Bull Run District. Brentsville's official mascot for all sporting events is the tiger, the school colors are black and orange. The high school athletic program has historically performed well in its wrestling and cross country programs and surprised with outstanding seasons in multiple sports over the years, several times resulting in unexpected state championships. While the football team has never been awarded a state championship, they have made it to the state tournament in recent years.