Tacchinardi joined Juventus in pre-season training in 1994. In his first season with the club, Tacchinardi made 25 league appearances, and also appeared in the Coppa Italia and the European Cup. Throughout his time with the club, he formed partnerships in midfield with the likes of Antonio Conte, Paulo Sousa, Didier Deschamps, Angelo Di Livio, Edgar Davids, Gianluca Zambrotta, Mauro Camoranesi, Pavel Nedvěd, Zinedine Zidane, and Enzo Maresca. During his time with the club, Tacchinardi was noted for his work-rate, determination, and wide range of skills.