In the "Scooby-Doo" franchise, DeLisle began voicing Daphne Blake with the direct-to-video film "Scooby-Doo and the Cyber Chase", a role she inherited from her friend and teacher Mary Kay Bergman following the latter's suicide. She later said of Bergman, "It was an interesting turn of events to get to play Daphne, but I'm so glad that I have the role, and I was glad that I was able to carry that on for her. She set the bar very high." DeLisle also credited the work for helping her maintain her music career, stating "I think Daphne just saved country music." In 2019, Warner Bros. announced that Amanda Seyfried would be voicing Daphne in the animated film "Scoob!" instead of DeLisle. Both DeLisle and Matthew Lillard, the voice of Shaggy Rogers since 2010, were upset about not being contacted about the decision to recast their roles for the film.