Kooistra and Bakker (2002), therefore, tested the hypothesis that such thawing is unnecessary. Seedlings of 3 species including interior spruce were planted with frozen root plugs (frozen seedlings) and with thawed root plugs (thawed seedlings). Thawed root plugs warmed to soil temperature in about 20 minutes; frozen root plugs took about 2 hours, ice in the plug having to melt before the temperature could rise above zero. Size of root plug influenced thawing time. These outplantings were into warm soil by boreal standards, and seedlings with frozen plugs might fare differently if outplanted into soil at temperatures more typical of planting sites in spring and at high elevations. Variable fluorescence did not differ between thawed and frozen seedlings. Bud break was no faster among thawed interior spruce seedlings than among frozen. Field performance did not differ between thawed and frozen seedlings.