Tiger quolls reproduce seasonally. They mate in midwinter (June/July), but females can breed as early as April. The mating behaviour of the tiger quoll is unique among the quoll species in that the female vocalises when in estrus and easily accepts the male's mounting. In addition, the female's neck swells up. Mating involves the male holding on the female's sides with his paws and holding on the neck with his mouth. Copulation can last as long as 24 hours. Females give birth with their hindquarters raised and their tails curled. For the time the young is in the pouch, a female rests on her sides. After the young have left the pouch, females stay in nests they have built. For their first 50–60 days of life, the young cannot see, so they rely on vocalisations and touch to find their mother or siblings. It stops when their eyes open after 70 days. Young are not carried on the back, but they do rest on their mother and cling to her when frightened. By 100 days the young become more independent of their mothers, and the mothers more aggressive towards their young.