Robert Polidori was born in 1951 in Montreal, Quebec, Canada, to a French-Canadian mother and a Corsican father. At age 9, Polidori’s family moved to the United States where his father worked as an engineer at Air Force bases and NASA installations. He grew up in Seattle, southern California, New Orleans, and Cocoa Beach, and first attended university in Florida in 1969. During his freshman year Polidori saw Michael Snow's film "Wavelength (1967 film)" and, inspired to study filmmaking, moved to New York City. The following year, he was hired by the legendary Jonas Mekas and worked as the theatre manager of the Anthology Film Archives. During this time, Polidori worked on four experimental films, exhibited in 1975 at the Whitney Museum of American Art. In 1980 he graduated with a Masters of Arts in film from State University of New York (Buffalo).