In 1924, Beebe went on another expedition to his Guiana research station of Kartabo, intending to continue the detailed documentation of the tropical ecosystem that he had begun at Kalacoon. The paper which finally resulted from this study was published in "Zoologica" in 1925 and was the first study of its kind in the developing field of tropical ecology. Beebe continued to battle depression during this trip to Kartabo, both over his earlier loss of Blair, and over the death of his mother Nettie, who had died shortly before the beginning of the expedition.