She also starred as Christine Hamilton in the horror film "Scream 3" (2000). From 2003 to 2004, she starred in the ABC drama "Threat Matrix" as Special Agent Frankie Ellroy Kilmer. In next television season she starred in another short-lived series, "E-Ring", on NBC. In 2007, Rutherford began starring as Lily van der Woodsen in the CW series "Gossip Girl". The series ended in 2012. Rutherford has also starred in a number of Lifetime movies. In 2016, Rutherford was cast in the ABC thriller "Quantico" playing the recurring role of Laura Wyatt. Since 2018, Rutherford has played the recurring role of Melissa Daniels in the reboot series "Dynasty".