As of 2010, the museum had over 260 vehicles. While most are from New England and other areas of the United States, trolleys from Canada, Australia, Japan, Germany, Hungary, England, Scotland, Italy, and several other countries are also in the collection. One of the motor buses the museum owns is Biddeford and Saco #31, the bus that replaced trolley #31 in 1939. The bus was donated to the museum by the bus company. The Seashore Trolley Museum continues to acquire new vehicles for the collection.