These difficulties to detect the presence of NUMT can be problematic. Translocated mitochondrial sequences in the nuclear genome have the potential to get amplified in addition to, or even instead of, the authentic target mtDNA sequence which can seriously confound population genetic and phylogenetic analyses since mtDNA has been widely used for population mapping, evolutionary and phylogenic studies, species identification by DNA barcode, diagnosis of various pathologies, and forensic medicine. This simultaneous amplification of NUMT with free extrachromosomal mtDNA, additionally, prevents one from determining the exact number of NUMT fragments in the genome of different organisms, such as "Aedes aegypti" mosquitoes, especially those in which extended translocation of mtDNA fragments occur. This makes the diagnosis of certain mitochondrial disorders challenging. For instance, a large NUMT pseudogene was found on chromosome 1, while more recent analysis of the same sequence led to a conclusion that sperm mtDNA has mutations that cause low sperm mobility. Another example would be the recent report describing a heteroplasmic mtDNA molecule containing five linked missense mutations dispersed over the contiguous mtDNA CO1 and CO2 genes in Alzheimer’s disease patients, however, the more recent studies using PCR, restriction endonuclease site variant assays, and phylogenic analysis proposed that the nuclear CO1 and CO2 sequences revealed that they diverged from modern human mtDNAs early in hominid evolution about 770,000 years before and these preserved NUMTs could cause Alzheimer's disease. One of the possible ways of preventing from such erroneous result is an amplification and comparison of heterogeneous sequence, comprises both mtDNA and nDNA, with the obtained results from Sanger sequencing of purified and enriched mtDNA as shown in figure 4. Although this method is easy and only a few primers are required, it will prevent from a substantial error in phylogenetic studies of a population and all the previously mentioned false results.