The trio melody may be repeated once at a softer dynamic, or may not be repeated at all. Typically, it is played quietly for the first or second playthrough, then features piccolos (or flutes, or other woodwinds) playing over the trio melody. In almost all cases the trio now modulates to the subdominant key of the march, meaning one flat is added to the key signature. The key is now flatter and this repeat will, with softer instrumentation, offer a relaxing feel from the previous volume. The contrast makes the trio more memorable as the new key is maintained to the end of the strain. (For marches starting in minor keys, the trio usually modulates to the relative major.)