"Tuscarora" was recommissioned later in the year and sailed on 2 November 1865 for the Pacific Ocean via Cape Horn. She served with the South Pacific Squadron from 1866 until May 1869. She stood off Valparaíso, Chile, during the Spanish bombardment on 31 March 1866 and was also present at Callao, Peru, when the Spanish shelled it on 2 May. In 1867, "Tuscarora" stopped at Tahiti and other islands of the Society group. She also touched at Fiji, where she received payment of awards made to United States citizens in 1855 and 1858 for injuries and losses sustained as a result of acts of the natives.