"Crash Twinsanity"s voice actors were cast and directed by Chris Borders. Clancy Brown, the previous voice actor for Cortex, had left the series due to his dissatisfaction with the video game industry's financial compensation for voice actors. Lex Lang was called in for an audition to replace Brown, and was given an explanation that VU Games considered Brown's performance to be "too mean". After Borders described Cortex to Lang and had him listen to signature samples of Brown's performance, he encouraged Lang to play Cortex as more flamboyant and self-absorbed. Lang eventually created a depiction of Cortex that was "master evil with a bit of a childish feminine side that leaks out in his tirades" that had everyone laughing at the lines and the character. "Monty Python's Flying Circus" was an additional influence on Lang's delivery as Cortex. Other cast members include Mel Winkler, Michael Ensign, Susan Silo, Debi Derryberry, Alex Fernandez, Dwight Schultz and Quinton Flynn. The full-motion videos of "Crash Twinsanity" were created by Red Eye Studios, who previously created the full-motion videos for "Crash Nitro Kart". The soundtrack of "Crash Twinsanity" was composed, performed, arranged and produced by a cappella band Spiralmouth, while Gabriel Mann recorded and mixed the soundtrack at Asylum Recording Studios in Los Angeles.