The town built by the DuBois brothers is by no means the beginning of the history of Duboistown. It is situated at the mouth of Mosquito Run on the banks of the West Branch Susquehanna River. A tribe of Susquehannock Indians had what appears to have been a fairly major settlement at the mouth of the creek. The early European settlers found the remains of an Indian village there. Arhaeologic evidence of earthenware, soapstone ware, pestles, hatchets, ornaments, and charms were found on the land that is across the river from Lycoming Creek and nearby where the Sheshequin Path crossed the river.