Comstock is most famous for being one of the first to bring her students and other teachers out-of-doors to study nature. In 1895, she was appointed to the New York State Committee for the Promotion of Agriculture. In this position, she planned and implemented an experimental course of nature study for the public schools. The program was approved for statewide use through the extension service of Cornell. She then wrote and spoke on behalf of the program, helped train teachers, and prepared classroom materials. Starting in 1897, she taught nature study at Cornell. Comstock was the first female professor at Cornell. However, she was denied full professorship for twenty years until 1920. (In 1911, Martha Van Rensselaer and Flora Rose became the first women with full professorship at Cornell.)