US 25/US 341/SR 27 closely parallels the Norfolk Southern rail line northwest from Glynn County into Wayne County and to the city of Jesup. The three highways enter the city as Cherry Street between their crossing of Penholoway Creek and their intersection with US 301/SR 23; US 25 splits north to join US 301/SR 23 toward Ludowici. Just north of the US 301/SR 23 intersection, US 341/SR 27 turns north onto Pine Street, a four-lane street with center turn lane that curves northwest and parallels Cherry Street through the city. The highways cross over CSX's Nahunta Subdivision between the Jesup Amtrak station and the orthogonal intersection of the CSX and Norfolk Southern lines. North of the CSX line, US 341/SR 27 intersects US 84/SR 38 (1st Street). The highways have an oblique intersection with SR 169 (Lanes Bridge Road) and collects the western end of Cherry Street as it leaves Jesup as Odum Highway. US 341/SR 27 travels through the town of Odum as its Main Street and crosses to the north side of the Norfolk Southern rail line in an S curve at the Wayne–Appling county line.