Route 27 intersects County Route 531 (Main Street) after and continues northeast on Middlesex Avenue, passing through a residential environment where the route carries about 22,414 vehicles daily. The road comes to a bridge over Conrail Shared Assets Operations' Port Reading Secondary line. The route crosses back into Edison Township and becomes the Lincoln Highway again, passing over the Rahway River. It heads through business areas and passes near the Thomas Alva Edison Memorial Tower and Museum, which is located on the site of Thomas Edison's Menlo Park laboratory. The route closely parallels the Northeast Corridor rail line and crosses into Woodbridge Township, widening to four lanes at this point. Route 27 passes by the Metropark station serving Amtrak and NJ Transit trains and interchanges with the Garden State Parkway near Iselin, where approximately 19,780 vehicles travel on Route 27 on a daily basis. Past the Garden State Parkway, Route 27 narrows back to two lanes and continues northeast alongside the Northeast Corridor, passing through a mix of residential and commercial development as a three-lane road with two northbound lanes and one southbound lane.