Subtelomeres carry out essential functions with Shugoshin protein. Shugoshin is a centromere protein for chromosome segregation during meiosis and mitosis. There are two types of Shugoshin protein: SGOL1 and SGOL2. Sgo1 is only expressed in meiosis 1 for centromeric cohesion of the sister chromosomes, while Sgo2, expressed in meiosis and mitosis, is responsible for the segregation of chromosomes at centromeres in the M phase. In fission yeast, Sgo2 is localized not only in centromeres, but also in subtelomeres. Sgo2 interacts with subtelomeres during interphase; middle of the G2 phase and plays a major role in forming "knob", which is a highly condensed chromatin body. Sgo2 remains in subtelomeres, whose cells lack telomere DNA. Sgo2 represses the expression of subtelomeric genes that is in a different pass-way from the H3K9me3- Swi6-mediated heterochromatin. Sgo2 has also repressive effects for timing of subtelomeres replication by suppressing Sld3, a replication factor, at the start of the replication. Thus, Sgo2 regulate gene expressions and replication to ensure proper subtelomeric gene expression and replication timing.