The last regular steam passenger service was hauled in August, 1964. From this time until closure of the line in 1980 passenger services were run using Rail Motors, initially with Walker railmotors but due to degrading track quality the Walkers were replaced by Diesel Electric Railmotors (DERMs) from 1978 onwards. After 9 December 1980 no services operated beyond Coldstream and the Healesville-Coldstream section of the line was officially closed to all traffic on 10 March 1983. The Healesville Railway Cooperative was established in 1984 to reopen the line and in 1985 was granted an 'Order In Council' for this section by the Victorian State Government to operate the line as a tourist railway. It was partially reopened as far as Yarra Glen for tourist charter services in 1986 following major bridgework. However, these services ceased by 1990 when the Healesville Railway Cooperative merged with the Yarra Valley Tourist Railway, who began running trolley services on the Healesville-Yarra Glen section.