Many colonists considered the Proclamation Line adjusted in 1768 by the Treaty of Hard Labour, which demarcated a border with the Cherokee nation running across southwestern Virginia, and by the Treaty of Fort Stanwix, by which the Iroquois Six Nations formally sold the British all their claim west of the Alleghenies, and south of the Ohio. However, this region (which included the modern states of Kentucky, and West Virginia, as well as southwestern Virginia) was still populated by the other tribes, including the Cherokee, Shawnee, Lenape, and Mingo, who were not party to the sale. The Cherokee border had to be readjusted in 1770 at the Treaty of Lochaber, because European settlement in Southwest Virginia had already moved past the 1768 Hard Labour line. The following year the Native Americans were forced to make further land concessions, extending into Kentucky. Meanwhile, the Virginian settlements south of the Ohio (in West Virginia) were bitterly challenged, particularly by the Shawnee.