Formed in 1929, Tuffley Rovers spent many years playing in the Stroud and District League and North Gloucestershire Association League before promotion to the Gloucestershire Northern Senior League and in the 1988–89 season they joined the Gloucestershire County League and were champions in 1991. In 1993, they were Hellenic League Division One champions and were promoted to the Premier Division. Under the management of Doug Foxwell they regularly finished in the top half of the Hellenic League, and were considered widely to be the second team in Gloucester, behind Gloucester City. However, during the 2005–06 season, they resigned from the Premier Division due to financial difficulties and their record was expunged.