Route 27 was part of the alignment through New Jersey of the Lincoln Highway, the United States' first transcontinental highway that was established in 1913. Route 27 is still referred to as the Lincoln Highway in many municipalities, such as Edison Township. The Lincoln Highway became part of pre-1927 Route 1 between New Brunswick and Elizabeth in 1916 and pre-1927 Route 13 between Trenton and New Brunswick in 1917. In 1927, the Lincoln Highway was renumbered as Route 27 between Trenton and Newark. U.S. Route 1 was designated on this portion of the road until it was relocated by the 1940s. U.S. Route 206 followed the route from Trenton to Princeton until 1953, when the southern terminus of Route 27 was cut back to Princeton to avoid the concurrency with U.S. Route 206.