In January 1967, "Charles H. Roan" was underway again for Guantanamo Bay, Cuba to undergo an Operational Readiness Inspection (ORI) along with other ships of Destroyer Squadron 10 (DesRon10). She spent a week of heavy exercising in the area of anti-submarine warfare and naval gunfire support prior to the scheduled ORI. She completed the ORI and proceeded to San Juan, Puerto Rico. From San Juan, she headed for Culebra where she did a naval gunfire support exercise and later on ASW exercises in the operation area. The Bahamas and Virgin Islands were visited on this cruise before she headed home to Newport. In March 1967, "Charles H. Roan" deployed to the Mediterranean again where she operated with the Sixth Fleet until the end of July. During this cruise she visited Tripoli, Libya, Valletta, Malta, Naples, Italy, Athens (Piraeus), Greece, Bodrum, Turkey, Cannes, France, Naples, Italy, Gibraltar, among others. "Charles H. Roan" also provided support to other US Navy ships during the Six-Day War, and assisted in escorting to Malta for repairs after her attack by Israeli forces.