Zamboni was born at Peschiera del Garda. She studied at the Il Conservatorio di Musica Arrigo Boito in Parma under M.Silva before making her professional opera debut in 1921 at Piacenza as Marguerite in Gounod's "Faust". Over the next few years she appeared at numerous major opera houses throughout Italy, including the Teatro Regio di Torino, Teatro di San Carlo, Teatro Regio di Parma, and Teatro Comunale di Bologna among others. Her roles included Violetta in Verdi's "La traviata", Desdemona in Verdi's "Otello", Ännchen in "Der Freischütz", the title role in Massenet's "Manon", and the title role in Puccini's "Tosca" among others.