On 25 April 1641, Easter Day, Ambrose and his congregation of around 150 people were surrounded at Morleys Hall, Astley by the Vicar of Leigh and his armed congregation of some 400. Father Ambrose surrendered, and his parishioners were released after their names had been recorded. The priest was restrained, then taken on a horse with a man behind him to prevent his falling, and escorted by a band of sixty people to the Justice of the Peace at Winwick, before being transported to Lancaster Castle.