On 23 October 2009, it was announced she would play Glenda Mitchell the mother of Ronnie Mitchell, Roxy Mitchell and Danny Mitchell in "EastEnders" after Jill Gascoine withdrew from the role during her first day on set. On 27 February 2011, it was announced that Barber was to leave "EastEnders" in March 2011. In February 2015, Barber admitted that she would consider an "EastEnders" comeback. The star commented: "I’ve always felt that Glenda left with a few unresolved issues. It would be fun to go back and create a little bit of havoc!". Glenda returned for two episodes on 1 January 2016. Barber returned to "EastEnders" full-time in January 2017 before leaving once again in February.