The aircraft was used in the reconnaissance role thanks to its long endurance, but it was very vulnerable to enemy fighters or even bombers. Perhaps its only air victory was in the Aegean, when a fighter stalled while chasing a Z.501. The aircraft was more often relegated to second-line duties. Sometimes, with well-trained crews, it was able to attack submarines, damaging several of them (perhaps six in total) and contributing to the destruction of two others. The aircraft had no advanced detection systems, only depth charges.