Garzoni's first known commission was in the city where she grew up, Rome. It was in 1616, from the chemist Giovanni Vorvino to paint a herbarium. Four years later in 1620 Garzoni arrived in Venice and painted a "Saint Andrew" for the Venetian Church of the Ospedale degli Incurabili. Garzoni stayed in Venice for a few more years and during that time attended the Calligraphy school of Giacomo Rogni. Shortly after her studies, Garzoni produced a book of cursive characters called the "Libro de'caratteri Cancellereschi Corsivi".