After "Laramie", Byington guest-starred in "Oh, Those Hats!", a 1963 episode of "Mister Ed", playing Karen Dooley, an influential Beverley Hills columnist. She later appeared as Mrs. Jolly on Dennis Weaver's NBC comedy-drama "Kentucky Jones", and as wealthy J. Pauline Spaghetti in an episode of "Batman" in 1966. Her role before her death from cancer was in 1967, as Larry Hagman's mother on NBC's "I Dream of Jeannie". Her final role was in 1968 as Mother General on ABC's "The Flying Nun", starring Sally Field.