In 1880, Van Nuys and James Boon Lankershim formed the Los Angeles Farming and Milling Company from the San Fernando Homestead Association. Isaac Van Nuys was its president and manager. The company had a four-story building for milling to produce flour, meals, cracked wheat, hominy and livestock feed. Van Nuys also served as vice-president of the Farmers and Merchants Bank, a director in the Union Bank of Savings, a director in the Los Angeles Pressed Brick Company, and owner of the Van Nuys Hotel erected in 1896 in Downtown Los Angeles.