He obtained a degree in medicine and philosophy in 1553 and started teaching logic and philosophy in 1554 at the University of Bologna. In 1559, he became professor of philosophy and in 1561 he became the first professor of natural sciences at Bologna ("lectura philosophiae naturalis ordinaria de fossilibus, plantis et animalibus"). Aldrovandi was a friend of Francesco de' Medici, Grand Duke of Tuscany (1574 – 1587), visiting his garden at Pratolino and travelling with him, compiling a list of the most valuable plants at Pratolino. He also formed fruitful associations with botanical artists such as Jacopo Ligozzi, to further develop illustrated texts. He died in Bologna on 4 May 1605, at the age of 82.