Holy Bull was retired to stud at Jimmy Bell's Jonabell Farm, which was later sold to Darley Stud and became part of their North American operation. Despite his unfashionable pedigree, he was considered a "blue collar" success with 70% of his runners going on to win. Three years after the time of his retirement from stud in 2012, he had sired 51 stakes winners. His offspring include 2000 Breeders' Cup Juvenile winner and U.S. Champion 2-year-old male Macho Uno, 2005 Kentucky Derby winner Giacomo, 2007 Stephen Foster Handicap winner Flashy Bull, and graded stakes race winners Bwana Bull, Confessional, Bishop Court Hill, Woke Up Dreamin, Eishin Boone, and Sunny Ridge.