After finishing her education, Garzoni and her brother Mattio left Venice in 1630 for Naples where she worked for the Spanish viceroy, the Duke of Alacala. Garzoni remained in Naples for a year before returning to Rome in 1631. Garzoni's stay in Rome was short lived however, due to Christina of France's persistent efforts to have the artist come to Turin to serve as the miniaturist for the Turinese court. Garzoni reached Turin in 1632 and lived there until 1637. After staying in Turin, Garzoni became familiar with the work of fellow artists Fede Galizia and Panfilo Nuvolone. A few years later in 1640, Garzoni arrived in Paris and stayed there until 1642 when she went to Rome. Garzoni traveled back and forth from Rome to Florence until 1651 where her primary clients were in the Medici Family, particularly Grand Duke Ferdinando II, Grand Duchess Victoria, and Cardinal Giovan Carlo.