In Fairport, NY 250 follows South Main Street down a large hill that levels off at the southern edge of the village's business district. Here, NY 250 intersects Church Street (NY 31F) and passes by the First Baptist Church of Fairport, located on the northwestern corner of the junction. The route continues north into the center of the village, passing by several small businesses and Fairport Village Landing—the largest shopping plaza in the village—before crossing over the Erie Canal by way of a lift bridge and becoming North Main Street. Once on the north side of the canal, NY 250 crosses the CSX Transportation-owned Rochester and West Shore Subdivisions at a single grade crossing ahead of High Street. At this point, the businesses give way to homes as the highway ascends a hill at the north end of the village and meets Whitney Road at the northern village line.