Basic conditions and detergents, such as SDS, can interfere with the dye's ability to bind to the protein through its side chains. However, there are some detergent-compatible Bradford reagents. The Bradford assay depends on the sequence of the protein. Thus, if the protein does not contain an ideal number of aromatic residues, then the dye will not be able to bind to the protein efficiently. Another disadvantage of the Bradford Protein Assay is that this method depends on comparing the absorbance of the protein to that of a standard protein. If the protein does not react to the dye in a similar way as the standard protein, it is possible that the concentration measured will be inaccurate.