Under real mode operating systems, such as DOS, calling INT 13h would jump into the computer's ROM-BIOS code for low-level disk services, which would carry out physical sector-based disk read or write operations for the program. In DOS, it serves as the low-level interface for the built-in block device drivers for hard disks and floppy disks. This allows INT 25h and INT 26h to provide absolute disk read/write functions for logical sectors to the FAT file system driver in the DOS kernel, which handles file-related requests through DOS API (INT 21h) functions.