During Ottoman rule Gračanica became an important cultural center. In the time of Metropolitan Nikanor (1528–1555) several icons were painted on the altarpiece. Also, because of the printing press, Nikanor obtained numerous service books and objects for monastic use. The royal doors were commissioned in 1564 by Metropolitan Dionisije, whose death is represented on a fresco in the narthex. Major restoration took place through efforts of Patriarch Makarije Sokolović. All the openings on the external narthex were walled up and new frescoes were completed in 1570. Thanks to Patriarch Pajsije, the church got its leaden roofing, and in 1620 the large cross with crucifix was made on the iconostasis. The monastery was exposed to new damages toward the end of the 17th century, in the Great Turkish War, after the second siege of Vienna - in which the Serbs took part on the Christian side. Turks removed the leaden cross and pulled out the floor tiles, together with the treasure hidden in the church by Patriarch Arsenije III.