The 10-month Siege of Florence (1529–1530) by the Spanish ended the Republic of Florence and Alessandro de' Medici became the ruler of the city. The siege brought the destruction of its suburbs, the ruin of its export business and the confiscation of its citizens' wealth. Alessandro, who ruled from 1531 to 1537, was the first Medici to use the style Duke of Florence, a title arranged for him in 1532 by Holy Roman Emperor Charles V. In 1569, Duke Cosimo I was elevated to the rank of Grand Duke of Tuscany by Pope Pius V. The Medici would continue to rule in Tuscany as grand dukes until 1737. After the Battle of Marciano in 1554, the city's historical rival Siena was conquered and the only remaining territory in Tuscany not ruled from Florence was the Republic of Lucca (later a Duchy).