In 2013, the NEI launched the Audacious Goals Initiative in Regenerative Medicine for Vision (AGI), originally the NEI Audacious Goals Initiative, to catalyze fundamental research toward “restoring vision through the regeneration of neurons and neural connections in the eye and visual system.” The initiative targets photoreceptors and retinal ganglion cells. Currently, the AGI funds three research consortia, representing 16 projects and $62 million. The AGI Functional Imaging Consortium addresses the technical needs and opportunities for imaging cells of the visual system as they respond to light. The AGI Regenerative Factor Discovery Consortium is identifying factors that control cell regeneration in the visual system. The Translational-Enabling Models Consortium is developing animal models that have fidelity to human eye disease, a critical step toward testing regenerative therapies in clinical trials. Beyond direct funding, AGI has generated interest from the vision research community, helping to expand the NEI regenerative medicine portfolio.