In the alternate reality designated Earth-65, Gwen Stacy is the one bitten by the radioactive spider, and becomes a superhero going by the name of Spider-Woman. She is also a member of a band fronted by Mary Jane Watson, simply called the Mary Janes. Shortly after Gwen begins fighting crime, Peter Parker, her best friend attempts to exact revenge on those who bullied him, becoming this universe's version of the Lizard. Gwen subdues him, but Peter dies towards the end of the battle due to the chemical he used. Spider-Woman is blamed for his death, causing an outcry for her arrest, led by J. Jonah Jameson. Her father, who is also a police chief in this world, begins a hunt for her. This follows Gwen into college, where she is still a member of the Mary Janes. At a gig of theirs, an assassin is sent after Gwen's father, who is in the audience. Gwen defeats the assassin, the audience and band clearing out during the battle. While they are alone, Captain Stacy holds Spider-Woman at gun point, with Gwen taking off her mask to reveal who she is. Shocked upon learning Spider-Woman's identity, he tells her to run before he changes his mind. In the distance, the Captain Britain from Earth-833, called Spider-UK, is watching, saying that Gwen will "do quite nicely".