Aquivaldo Mosquera made his debut in 2000 playing for Atlético Nacional. He would make over 150 appearances for the club before moving to Pachuca of Mexico in 2005. With Pachuca, he won two league titles (Clausura 2006 and 2007), a CONCACAF Champions' Cup in 2007, and a Copa Sudamericana in 2006. In the summer of 2007 Mosquera transferred to Sevilla FC of La Liga. Though he would stay with the club until 2009, Mosquera only managed to appear in 41 league matches.