The hyaline layer is responsible for the adhesion and proper orientation if the cells of an embryo. Throughout development, certain cells change their binding affinity towards hyalin. Hyalin helps cells differentiate into the animal and the vegetal halves during oogenesis by utilizing zinc and lithium ions. Zinc enhances the amount of hyalin precipitated, while lithium keeps the hyalin solubilized when around calcium. Zinc, then, causes an animalizing effect since the binding of the blastula cells would be stronger, while the weaker attachment of the cells would form the vegetal half.