They transport the injured Neville back to his home, where Anna explains that they survived the outbreak aboard a Red Cross evacuation ship from São Paulo, and are making their way to a survivors' camp in Bethel, Vermont. Neville denies this and argues that no such survivors' camp exists. Neville adjusts the potential cure and injects the female Darkseeker. The next night, a group of Darkseekers, who tracked Anna and Neville the night before, invade the house. Neville, Anna, and Ethan retreat into the basement laboratory sealing themselves in with the female test subject. Discovering that the last treatment was successful, Neville assesses the situation as the Darkseeker alpha male rams himself against a glass door to break in. Neville draws a vial of blood from the woman he cured, and gives it to Anna, before shutting Ethan and her inside a coal chute in the back of the lab. Neville then takes a grenade and kills the Darkseekers at the cost of his own life, saving the cure.