In tandem with the use of the new Woody design, "The Barber of Seville" was the first Woody Woodpecker cartoon to use the standardized opening title card, animated by Hawkins, featuring Woody popping out of a log, asking "Guess Who?!", and delivering his trademark laugh. The audio for this opening sequence is lifted from Woody's first starring appearance in the 1941 cartoon "Woody Woodpecker", in which Woody was voiced by Mel Blanc. Ben Hardaway, also the co-storyman on "Barber of Seville", provides Woody's voice for the first time (replacing Kent Rogers, who was killed in a World War II plane crash three months after this short's release), and Lee Sweetland performs as Woody's singing voice. Hardaway would become Woody's sole speaking voice for the remainder of the decade. Finally, "The Barber of Seville" was the first Woody cartoon directed by veteran animator James Shamus Culhane, who had been working on Lantz's "Swing Symphonies" for a year prior, Culhane would continue to direct entries in the series until 1946.