Du Toit first competed internationally at the age of 14, when she took part in the 1998 Commonwealth Games in Kuala Lumpur. During the 2002 Commonwealth Games in Manchester, Du Toit, who was then 18 years old, won both the multi-disability 50 m freestyle and the multi-disability 100 m freestyle in world record time. She also made sporting history by qualifying for the 800 m able-bodied freestyle final – the first time that an athlete with a disability had qualified for the final of an able-bodied event. At the closing of the Manchester Commonwealth Games, she was presented with the first David Dixon Award for Outstanding Athlete of the Games.