In 1993, Amir began studying at Bar-Ilan University as part of its kollel program, mixing religious and secular studies. Amir studied law and computer science, as well as Jewish law at the Institute for Advanced Torah Studies. Amir was strongly opposed to the Oslo Accords. He participated in protest rallies against the accords on campus, was active in organising weekend bus outings to support Israeli settlers, and helped found an illegal settlement outpost. He was especially active in Hebron, where he led marches through the streets.