Although an increasing number of studies have been focused on plastic debris accumulation on the coasts, in off-shore surface waters, and that ingested by marine organisms that live in the upper levels of the water column, there is limited information on debris in the mesopelagic and deeper layers. Studies that have been done have conducted research through bottom sampling, video observation via remotely operated vehicles (ROVs), and submersibles. They are also mostly limited to one-off projects that do not extend long enough to show significant effects of deep-sea debris over time. Research thus far has shown that debris in the deep-ocean is in fact impacted by anthropogenic activities, and plastic has been frequently observed in the deep-sea, especially in areas off-shore of heavily populated regions, such as the Mediterranean.