The first Twyford station opened on 1 July 1839 and was the terminus of the GWR until 30 March 1840, pending the completion of Sonning Cutting. It was a timber building to the north of the line at right angles to the track. The actual platform was on a loop off the running line and served trains running in either direction, of which there were nine per weekday. Just to the west was a temporary engine shed, moved here from Maidenhead, the first terminus. After the opening to Reading the shed was removed and a platform was provided on the south side offset to the west. The line was crossed by a footpath between the platform ends.