A select few processes occur during early neuronal development in order to ensure proper calyx formation, specifically through the influence of Fibroblastic Growth Factor (FGF), transcription factor Math5, neural cell recognition molecule NB-2, and ephrin (Eph) proteins in cells. Math1/Math5 and FGF are two regulators essential for appropriate growth and development of the cochlear nucleus complex, which comprises both the ventral cochlear nucleus (VCN) and the dorsal cochlear nucleus (DCN). Sufficient FGF levels ensure proper morphology of the cochlear nuclei, while Math5 insures correct size and processing of the cochlear nucleus. Math1, another transcription factor, is necessary for the appearance of VCN neurons in the cochlear extramural stream as well as the neurons of the superior olivary complex. NB-2 also assists in the advancement of the formation of the calyx of Held, as well as contributing to the upkeep of the contralateral MNTB. The combined effects of these three molecules with one another illustrate the fact that there are many families of proteins involved in proper signaling and formation of the calyx.