Homebush Railway Station Group has historical significance at the state level representing three significant historical phases in the development of the NSW railways. Established during the first phase of NSW railway construction in the 1850s the site is significant as one of only four original intermediate stations on the first railway line in NSW between Sydney and Parramatta. The subsequent (second) rebuilding of the station in the 1860s was associated with servicing the nearby Government Abattoirs at Homebush. The existing (third) station arrangement with its extant platforms, station buildings and signal box dates from the 1891 quadruplication of the line represents the expansion of the railways in the late 19th century to accommodate increasing rail services. The existing 1890s platform buildings are historically significant as they are able to demonstrate the first use of island platforms in NSW. In addition the signal box, footbridge and brick store rooms collectively demonstrate a former era of travel, communication and trade.