Overall, there are two ultrastructural changes that occur in the calyx of Held. The first is that in the second week of development, myelination of the VCN axons in the MNTB increases. This prominent growth in myelin corresponds to the chronological development of the signaling circuit and adaptation of the calyx. The second ultrastructural change involves the principal neurons of the MNTBs, whose cell bodies and nuclei increase in surface area due to enlargement. This is a direct result of individual, larger postsynaptic densities breaking up into smaller, multiple densities.