In the 1994 European elections, Roth was again elected to the European Parliament as a lead candidate of Alliance 90/The Greens. She was chairperson of the Green Group in the European Parliament until 1998, first alongside co-chairman Alexander Langer (1994–1995) and later Magda Aelvoet (1995–1998). During this second term as an MEP, she was again a member of the European Parliament Committee on Civil Liberties, Justice and Home Affairs, the Sub-Committee on Human Rights and the EC-Turkey Joint Parliamentary Committee, of which she was elected deputy chairperson. She also remained involved with the Committee on Foreign Affairs as a substitute member.