The unit was established as No. 2 (Fighter) Operational Training Unit (No. 2 OTU) in April 1942 at Port Pirie, South Australia, and relocated to RAAF Station Mildura, Victoria, the following month. During World War II, it provided training on a wide range of aircraft, including P-40 Kittyhawks, Vultee Vengeances, Avro Ansons, CAC Boomerangs, Supermarine Spitfires and Airspeed Oxfords. Disbanded in March 1947, No. 2 OTU was re-formed at Williamtown in March 1952 in response to the demand for more highly trained pilots to serve in the Korean War. It was renamed No. 2 (Fighter) Operational Conversion Unit in September 1958, and since then has conducted training with the CAC Sabre, Dassault Mirage III, and Macchi MB-326, prior to taking delivery of the Hornet.