Though there is no explicit confirmation of this, it is considered very likely for Margaret to have served as a maid-of-honour to the queen, Catherine of Saxe-Lauenburg: she was fifteen years old in 1531 when the king married Catherine. Her sister Brita married the king's favorite courtier Gustav Olofsson Stenbock in the presence of the royal couple shortly after, and though no list of Queen Catherine's ladies survives, Margaret's social position, connections, age, and contemporary custom makes it extremely likely that she completed her education by serving the queen, as was the custom for girls of her position at the time.