The Hightstown High School Rams compete in the Colonial Valley Conference, which is comprised of public and private high schools in Mercer, Middlesex and Monmouth counties, operating under the supervision of the New Jersey State Interscholastic Athletic Association (NJSIAA). With 1,218 students in grades 10-12, the school was classified by the NJSIAA for the 2019–20 school year as Group IV for most athletic competition purposes, which included schools with an enrollment of 1,060 to 5,049 students in that grade range. The football team competes in the Valley Division of the 95-team West Jersey Football League superconference and was classified by the NJSIAA as Group IV South for football for 2018–2020.