On October 20, 2016, the Steelers lost to the New England Patriots and Timmons racked up a season-high 11 solo tackles during the game. In Week 11, Timmons recorded eight combined tackles and made his first sack of the season in a 24–9 victory over the Cleveland Browns. During Week 13, he made seven combined tackles and intercepted New York Giant's quarterback Eli Manning and returned it 58-yards. He finished the season with 114 combined tackles, 2.5 sacks, 5 pass deflections, two interceptions, and a forced fumble in 16 games and 16 starts.