He started the season making five solo tackles in a 19–31 loss to the Denver Broncos. During a Week 6 contest against the Tennessee Titans, he made six total tackles and intercepted Titans quarterback Matt Hasselbeck for his first pick of the season. On November 25, 2016, Timmons racked up ten combined tackles and intercepted Brandon Weeden and scored a 52-yard touchdown for the first of his career. The Steelers went on to lose to the Browns 14–20. During Week 14, he made a season-high 11 solo tackles in a loss to the San Diego Chargers. He finished his sixth NFL season with a total of 106 tackles, 6.0 sacks, 2 forced fumbles and a career-high 3 interceptions. This was his first season starting all 16 regular season contests.