During the 2005–06 season, Neuner made her first appearances in the Biathlon World Cup. Germany's women's national coach Uwe Müßiggang had already considered her for the team two years earlier, however, her parents and her hometown coaches Bernhard Kröll and Herbert Mayer were reluctant to let her start prematurely. On 13 January 2006, Neuner made her debut in the World Cup sprint race in Ruhpolding, Germany, where she substituted for the injured Uschi Disl. Although her first appearance ended unsuccessfully, coming in 41st place, she was appointed for nine more World Cup races for the remainder of the season.