The concentration of ammonium in the waters of Buffalo Creek ranges from 0.01 milligrams per liter to almost 0.12 milligrams per liter. Not far from the headwaters, the concentration is at its lowest, but it rises to its peak at Buffalo Flat Road. The concentration then falls to 0.02 milligrams per liter at Aikey Road, before rising to 0.05 milligrams per liter at Johnson Mill Road, near the confluence of Spruce Run and Buffalo Creek. The concentration then fluctuates slightly between there and the mouth of Buffalo Creek. The amount of nitrogen flowing through Buffalo Creek and all its tributaries in 2000 and 2008 was approximately per year.