As a Bible scholar, Toscano explores LGBTQ issues. He is especially known for highlighting gender non-conforming characters in the Bible. In his performance lecture, "Transfigurations--Transgressing Gender in the Bible", he focuses on eunuchs and non-eunuchs who transgress and transcend gender. He first premiered Transfigurations in November 2007 and in March 2017 released the material as a film directed by Samuel Neff. The film was accepted for 2017 and 2018 LGBT film festivals including the 2017 Queer Kampala International Film Festival in December 2017. Moments before Toscano's film was scheduled to screen though, law enforcement agents raided the festival and shut it down. In response, Toscano offered the film for free on-line for a month and festival organizers arranged for a second showing in Kampala at an undisclosed location in January.