John Winchester gives it to the demon Azazel in return for Dean's life being spared after a near-fatal car crash. At the end of the second season, Azazel uses the Colt as the key to open one of the Gates to Hell that Samuel Colt had sealed. The last bullet is then used to kill Azazel, though the gun is later altered to allow the use of more bullets. Towards the end of the third season, Lilith's right-hand demon, Crowley, acquires the gun and hides it. It is then featured in two time-travel episodes – Dean using a past version of it when he is sent back to 1973, and his future self discovering it when Dean is sent five years into the future – before Crowley returns it to the Winchesters so they can kill Lucifer. However, after Dean shoots Lucifer in the head at point-blank range, an unharmed Lucifer boasts there are five things in creation which the gun cannot kill, and he is one of them. The Colt is subsequently lost and absent for several years until Mary Winchester retrieves it back from a Prince of Hell, Ramiel.