Due to the less stable structure of mesophiles, it has reduced flexibility for protein synthesis. Mesophiles are not able to synthesize proteins in low temperatures. It is more sensitive to temperature changes, and the fatty acid composition of the membrane does not allow for much fluidity. Decreasing the optimal temperature of 37 °C to 0 °C to 8 °C leads to a gradual decrease in protein synthesis. Cold-induced proteins (CIPs) are induced during low temperatures, which then allows cold-shock proteins (CSPs) to synthesize. The shift back to the optimal temperature sees an increase, indicating that mesophiles are highly dependent on temperature. Oxygen availability also affects microorganism growth.