Buckland studied at Christ Church from 1844–48, graduating at the second attempt. Passing out in May 1848 and at the advice of Richard Owen and Sir Benjamin Brodie, his father sent him to study surgery in London at St George's Hospital under Caesar Hawkins. He attended classes by Henry Gray where another classmate was Francis Day. During this time he also became acquainted with Abraham Dee Bartlett who would send him dead animals at the zoo and he continued to keep many animals. A visit to Paris in 1849 gave him a chance of comparing their methods with those in London. In London most of the nurses were illiterate; one who claimed to read was tested with a label reading "This lotion to be applied externally only".