At the end of 2005, Dallaglio announced that he was making himself available again for England selection and he was included in the squad for the 2006 Six Nations Championship. He was named on the bench for England's opening match of the tournament against Wales on 4 February at Twickenham. He was a blood replacement for clubmate Joe Worsley after 13 minutes and on 64 minutes was a replacement for Martin Corry, touching down with a clinically executed charge from the back of the scrum on 70 minutes. He gained three more caps off the bench during the tournament, but did not play in the last game of the championship, when England lost 28–24 to Ireland.