The different genetic targets of the "ain" and "lux" systems are essential, because these two systems respond to different cellular environments. The "ain" system regulates transcription in response to intermediate cell density cell environments, producing lower levels of luminescence and even regulating metabolic processes like the acetate switch. On the other hand, the "lux" quorum sensing system occurs in response to high cell density, producing high levels of luminescence and regulating the transcription of other genes, including QsrP, RibB, and AcfA. Both of the "ain" and "lux" quorum sensing systems are essential for colonization of the squid and regulate multiple colonization factors in the bacteria.