After experiencing a minor hamstring strain during spring training, Reyes began the 2014 season batting leadoff for the Blue Jays. In his first at bat on Opening Day he aggravated his hamstring injury while running to first base, and was placed on the 15-day disabled list at the end of the game. Reyes was activated on April 19, after playing in two rehab games with the High-A Dunedin Blue Jays. He avoided the disabled list for the rest of the season, playing in 143 games and batting .287 with 9 home runs, 51 RBI, and 30 stolen bases.