Fenn won her most famous role and made an impression on the public when she was cast by David Lynch and Mark Frost as the tantalizing, reckless Audrey Horne, a high school "femme fatale", in the TV series "Twin Peaks". The series ran from 1990 to 1991, and the character of Audrey was one of the most popular with fans, in particular for her unrequited love for FBI Special Agent Dale Cooper (played by Kyle MacLachlan) and her style from the 1950s (with her saddle shoes, plaid skirts, and tight sweaters). Fenn reached cult status with a scene in which she danced to Angelo Badalamenti's music and a scene in which she knotted a cherry stem in her mouth. "With Sherilyn Fenn, "Twin Peaks" came on and effortlessly destroyed every other show's sexuality", said James Marshall, one of her cast-mates. Speaking in September 1990, Fenn pointed out: "Audrey is a woman-child who dresses like the girls in the '50s and shows her body. But she's daddy's little girl at the same time." In the show's second season, when the idea of pairing Audrey with Cooper was abandoned, Audrey was paired with other characters like Bobby Briggs (played by Dana Ashbrook) and John Justice Wheeler (Billy Zane). About Audrey, Fenn said: