The former Enterprise Branch (E Branch) was built in 1885 by the Atlantic Coast, St. Johns and Indian River Railroad and leased to the Jacksonville, Tampa and Key West Railroad, part of the Plant System. Initially, the westernmost five miles (8 km) served as a connection from Enterprise Junction to Enterprise, a port for steamboat traffic down the St. Johns River. Later, the line was built through Osteen, Kalamazoo, and Mims to Titusville. The Enterprise Branch also crossed the Kissimmee Valley Line at a location known as Maytown.