The interior features rich colours and strident colour contrasts, characteristic of Butterfield's work, compared to the exterior. All the stonework is constructed of the Waurn Ponds limestone with contrasting stripes of the very dark-coloured local bluestone. The dado, floor, high altar and reredos are outstanding examples of High Victorian Gothic polychromy. The reredos is made from Devonshire marble, alabaster and glittering Venetian glass mosaics. One of the carved figures on the pulpit is said to be the image of a former Mayor of Melbourne's daughter who died in infancy. The floor is entirely paved with encaustic tile imported from the English firm of Maw & Co., featuring both patterned layouts and patterns within the tiles, while the dado is created with patterned glazed tiles.