In 1489, by permission of Pope Innocent VIII, the sisters adopted the Cistercian rule, bound themselves to the daily recitation of the Office of the Immaculate Conception, and were placed under obedience to the ordinary of the archdiocese. In 1501, Pope Alexander VI united this congregation with the Benedictine community of San Pedro de las Duenas, under the Rule of St. Clare, but in 1511 Julius II gave it a rule of its own, and in 1616 special constitutions were drawn up for the congregation by Cardinal Francisco de Quiñones.