The First Nation have reserved for themselves two areas. Their main reserve, the Alderville First Nation (formerly designated as Alderville Indian Reserve 37 by Indian and Northern Affairs Canada), is located near the south shores of Rice Lake in Ontario (). It consists of six non-contiguous areas within the Alnwick/Haldimand Township approximately north of Cobourg, and occupies a total area of . They also maintain a smaller parcel of land on nearby Sugar Island, the Sugar Island 37A Indian Reserve (). It has been home to the Mississauga division of the Anishinaabe (Ojibwa) Nation since the mid-1830s.