The speed and power of the French advance frightened the other Italian rulers, including the Pope and even Ludovico of Milan. They formed an anti-French coalition, the League of Venice on 31 March 1495. The formation of the League of Venice, which included the northern Italian states of Duchy of Milan, the Republic of Venice, the Duchy of Mantua, and the Republic of Florence in addition to the Kingdom of Spain, the Holy Roman Empire and the Kingdom of Naples, appeared to have trapped Charles in southern Italy and blocked his return to France. Charles would have to cross the territory of at least some of the League members to return home to France. At the Fornovo in July 1495, the League was unable to stop Charles from marching his army out of Italy. The League lost 2,000 men to his 1,000 and, although Charles lost nearly all the booty of the campaign, the League was unable to stop him from crossing their territory on his way back to France. Meanwhile, Charles' remaining garrisons in Naples were quickly subdued by Aragonese forces sent by Ferdinand II of Aragon, ally of Alfonso on 6–7 July 1495. Thus in the end, Charles VIII lost all the gains that he had made in Italy in 1494.