On 1 July 1959, Barbero was reassigned to the Pacific Fleet's Submarine Squadron 1, based at Pearl Harbor to conduct deterrent patrols in the Pacific due to a shift in policy placing all deployed Regulus assets there. She usually patrolled with "Tunny" to provide four missiles on station. In January 1962, "Barbero" entered the Pearl Harbor Naval Shipyard for an extensive five-month overhaul. During that time, members of her crew pursued various fields of training and education at service schools in Hawaii and on the United States West Coast. After concluding the overhaul in June 1962, "Barbero" carried out local operations and conducted refresher training. She then completed a single deterrent patrol in the latter half of 1962. "Barbero" conducted another two deterrent patrols in 1963, making liberty calls in East Asian ports at the conclusion of each.