ECs derived from stem cells are the beginning of vasculogenesis. Vasculogenesis is new production of a vascular network from mesodermal progenitor cells. This can be distinguished from angiogenesis, which is the creation of new capillaries from vessels that already exist through the process of splitting or sprouting. This can occur "in vitro" in embryoid bodies (EB) derived from embryonic stem cells; this process in EB is similar to "in vivo" vasculogenesis. Important signaling factors for vasculogenesis are TGF-β, BMP4, and VEGF, all of which promote pluripotent stem cells to differentiate into mesoderm, endothelial progenitor cells, and then into mature endothelium. It is important to talk more about vasculogenesis because this is what makes ECs different from other types of cells that are found in the body. During vasculogenesis, the heart and vascular plexus form while the organism is still an embryo, compared to anigiogenesis which is essentially the extension of this. Another difference major difference between the two formation processes is that vasculogenesis originates from hemangioblasts, which come from the mesoderm. There is also differences that occur in the signaling pathways of these two pathways that makes them noticeably different.