A graduate of the University of California, Berkeley (BA and PhD), Wilson received his doctorate under the supervision of Ernest Lawrence for his work on the development of the cyclotron at the Berkeley Radiation Laboratory. He subsequently went to Princeton University to work with Henry DeWolf Smyth on electromagnetic separation of the isotopes of uranium. In 1943, Wilson and many of his colleagues joined the Manhattan Project's Los Alamos Laboratory, where Wilson became the head of its Cyclotron Group (R-1), and later its Research (R) Division.