Bacterial growth is predominant in petroleum-based cutting fluids. Tramp oil along with human hair or skin oil are some of the debris during cutting which accumulates and forms a layer on the top of the liquid; anaerobic bacteria proliferate due to a number of factors. An early sign of the need for replacement is the "Monday-morning smell" (due to lack of usage from Friday to Monday). Antiseptics are sometimes added to the fluid to kill bacteria. Such use must be balanced against whether the antiseptics will harm the cutting performance, workers' health, or the environment. Maintaining as low a fluid temperature as practical will slow the growth of microorganisms.