On December 16, 2007, Sproles ran one yard for his first career rushing touchdown against the Detroit Lions. This was followed by an 11-yard run for his second rushing touchdown, capping off his first career 100+ yard rushing game. Overall, he finished the 2007 regular season with 164 rushing yards and two rushing touchdowns to go along with 10 receptions for 31 yards. On special teams, he had 37 kick returns for 1,008 net yards and a touchdown to go along with 24 punt returns for 229 net yards and a touchdown. The Chargers made the playoffs in 2007. In the Divisional Round against the Indianapolis Colts, he had a 56-yard receiving touchdown in the 28–24 victory. He had 34 rushing yards in the AFC Championship loss to the New England Patriots in the next round.