Meanwhile, Irene pledges her love to Tamerlano if he returns his own love to her, while Leone hopes that ultimately love will win the day over revenge. Tamerlano prepares to humiliate his enemies, and begins by bringing Bajazet and then Asteria before him. Andronico pleads for mercy from Tamerlano. Tamerlano will not listen, and orders Asteria to become his servant, and that she must first serve him wine. Asteria puts the poison from her father into the cup. Irene then reveals her true identity to Tamerlano and the situation of the poisoned cup. Tamerlano then orders Asteria to decide who first to give the cup to, her father or Andronico, before she may bring it to him. Asteria is about to drink from the cup herself when Andronico hits it out of her hand, which further enrages Tamerlano. He orders Asteria's arrest and confinement in the common seraglio, and that Bajazet will have to see her being shamed. Bajazet, angry at this situation, declares that his spirit will haunt Tamerlano when he has died. (Aria:""Empio, per farti guerra"".)