Ferdinand died on 25 January 1494, worn out with anxiety; he was succeeded by his son, Alphonse, Duke of Calabria, who was soon deposed by the invasion of King Charles which his father had so feared. The cause of his death was determined in 2006 to have been colorectal cancer (mucinous adenocarcinoma type with mutation in the KRas gene), by examination of his mummy. His remains show levels of carbon 13 and nitrogen 15 consistent with historical reports of considerable consumption of meat.