On January 3, 2009, during an AFC Wild Card Round game against the Indianapolis Colts, Sproles ran for two touchdowns in place of the injured LaDainian Tomlinson, including the game-winning touchdown in overtime to beat the Colts 23-17. With 105 yards rushing, 45 yards receiving and 178 return yards, Sproles finished the game with 328 all-purpose yards in the Chargers victory over the Colts, the third most all-purpose yards by a player in a single NFL post-season game in NFL history. He was named the NBC Sunday Night Football "Horse Trailer Player of the Game" for the second consecutive week. The week following however, Sproles was stopped by the Pittsburgh Steelers. He managed only 15 yards on 11 carries, but did have 5 receptions for 91 yards including a 62-yard touchdown.