Vittoria is put on trial for the murder of her husband and although there is no real evidence against her, she is condemned by the Cardinal to imprisonment in a convent for penitent whores. Flamineo pretends madness to protect himself from awkward suggestions. The banished Count Lodovico is pardoned and returns to Rome; confessing he had been secretly in love with Isabella, he vows to avenge her death. Isabella's brother Francisco also plots revenge. He pens a love letter to Vittoria, intentionally allowing it to fall into the hands of Brachiano, in order to fuel his jealousy. Though at first his plan seems to work, Vittoria manages to convince Brachiano that she is faithful and the two elope. Cardinal Monticelso is elected Pope and as his first act he excommunicates Vittoria and Brachiano, who have fled Rome.