Louisa Lane was born in London, England, the daughter of Eliza Trentner (1796–1887), a singer and actress, and Thomas Frederick Lane (1796–1825), an actor and theatre manager. Louisa and her mother came to America when she was six years old. She proved to be a child prodigy playing five different adult roles within one play at the age of eight in 1828. As a young woman and strolling player, her theatrical travels took her, her mother and half sisters as far away as Jamaica, by sailing ship, where one of her step fathers died. She returned to the United States in 1847 to support Junius Brutus Booth. She appeared in several plays with both him and his son, John Wilkes Booth.