In 1653, Groseilliers travelled to Acadia to meet with Claude de la Tour and returned to New France in July and establishing himself at Trois-Rivières. There he married his second wife, Marguérite Hayet, (sometimes spelled Hayot) the widowed step-sister of Pierre-Esprit Radisson and from whom he inherited his lands in Trois-Rivières. Two sons from her first marriage became troublesome and in 1654, Groseilliers petitioned the governor of New France to find the boys another guardian, which was accepted. His first child by Marguérite, Jean Baptiste, was baptized on 5 July 1654. His second child by Marguérite, also named Marguérite, was baptized on 15 April 1659 and his third child, Marie-Antoinette, was baptized on 8 June 1661.