Nyberg was in the crew of STS-124, which flew to the ISS in May 2008. This was the second of three flights to deliver components to complete the Japanese Kibō laboratory. In May 2009, she was assigned to the STS-132 mission, which launched in May 2010, but had to be replaced three months after assignment due to a temporary medical condition. Nyberg then served in a technical role until she received her next assignment, as a flight engineer on the Expedition 36/37.