Ahead of the 2009–10 season, Samba was linked a move away from Blackburn Rovers, with clubs around Europe interested in signing him, but both Manager Sam Allardyce and the player, himself, dismissed of him leaving the club. He continued to start the season, regaining his first team place in the centre–back position. Samba scored his first goal of the 2009–10 season against Aston Villa on 26 September 2009. However, he was sidelined on two separate occasions throughout October. Despite this, Samba signed a new five-year contract with the club. Shortly after signing a contract extension, local newspaper The Lancashire Telegraph reported that Samba "must go down as one of the bargains in Premier League history". He then scored on his 100th Premier League start in a 2–0 victory over Fulham at Ewood Park on 17 January 2010. Three days later on 20 January 2010, however, Samba was sent–off in the 39th minutes for unprofessional conduct, as Blackburn Rovers lost 6–4 to Aston Villa in the second leg of the League Cup semi–finals. After serving one match suspension, he was sent–off for a second bookable offence, in a 3–0 loss against Stoke City on 6 February 2010. After serving a two match suspension, Samba returned to the starting line–up, starting the whole game, in a 2–0 loss against Liverpool on 28 February 2010. He then netted his third league goal of the 2009–10 season on 13 March 2010 against in a 1–3 defeat to Tottenham at White Hart Lane. Samba featured for the next five matches before suffering an injury and was substituted in the 33rd minutes during a 0–0 draw against Manchester United on 11 April 2010. On 3 May 2010, Samba scored the winning goal as Rovers beat Arsenal 2–1 at Ewood Park. He was also picked as captain for the game, despite club captain Ryan Nelsen and vice-captain David Dunn both starting. For his performance, Samba was awarded the Unsung Hero at the club’s award ceremony. At the end of the 2009–10 season, Samba went on to make thirty–three appearances and scoring four times in all competitions.