US 250 continues east as Ivy Road, which crosses the Mechums River and passes under the railroad again in the namesake community. The highway parallels the railroad to just west of Charlottesville, where US 250 meets US 29 (Monacan Trail Road) at a partial cloverleaf interchange. US 250 Business continues east along Ivy Road through the University of Virginia campus while US 250 joins US 29 northward on the freeway bypass. The two highways have a partial interchange with Old Ivy Road, a northbound right-in/right-out interchange with Leonard Sandbridge Road, which serves the university, and a diamond interchange with Barracks Road at the western city limit of Charlottesville. US 250 and US 29 diverge at a partial cloverleaf interchange with Emmet Street, which heads south as US 29 Business. East of US 29, US 250 becomes an expressway that features a combination diamond and right-in/right-out interchange with Dairy Road and Meadowbrook Heights Road, and a partial cloverleaf interchange with the eastern end of Rugby Road at the highway's crossing of Norfolk Southern's Washington District. The highway also has diamond interchanges with McIntire Road/John W. Warner Parkway, Park Street and Locust Avenue, east of which the highway continues as Long Street.