In 546 BC, Chios was subjected to the Persian Empire. Chios joined the Ionian Revolt against the Persians in 499 BC. The naval power of Chios during this period is demonstrated by the fact that the Chians had the largest fleet (100 ships) of all of the Ionians at the Battle of Lade in 494 BC. At Lade, the Chian fleet doggedly continued to fight the Persian fleet even after the defection of the Samians and others, but the Chians were ultimately forced to retreat and were again subjected to Persian domination.