The median lethal dose, or LD50, of alpha-thujone, the more active of the two isomers, in mice, is around 45 mg/kg, with 0% mortality rate at 30 mg/kg and 100% at 60 mg/kg. Mice exposed to the higher dose have convulsions that lead to death within 1 minute. From 30 to 45 mg/kg, the mice experience muscle spasms in the legs, which progress to general convulsions until death or recovery. These effects are in line with other GABA antagonists. Also, alpha-thujone is metabolized quickly in the liver in mice. Pretreatment with GABA positive allosteric modulators like diazepam, phenobarbital, or 1 g/kg of ethanol protects against a lethal dose of 100 mg/kg.