As an extension, one can fit the data to different stoichiometries to understand the kinetics of the binding events between the host and the guest. made use of this corollary to slightly modify the conventional Benesi–Hilderbrand plot to get the order of the complexation reaction between barium-containing crown ether bridged chiral heterotrinuclear salen Zn(II) complex formula_57 (host) with various guests imidazoles and amino acid methyl esters, along with the other parameters. They titrated a fixed concentration of the zinc complex with varying amounts of the imidazoles and methyl esters whilst monitoring the changes in the absorbance of the pi-to-pi* transition band at 368 nm. The data fit a model in which the ratio of guest-to-host is 2 in the complex. They further carried these experiments at various temperatures which enabled them to calculate the various thermodynamic parameters using the van 't Hoff equation.