Enrichment is quantified from the measurement of isotope ratios, the ratio of the tracer isotope to a reference isotope, by mass spectrometry. Multiple definitions and calculations of enrichment have been adopted by different researchers. Calculations of enrichment become more complex when multiple tracers are used simultaneously. Because enriched isotope preparations are never isotopically pure, i.e. they contain all the element’s isotopes in unnatural abundances, calculations of enrichment of multiple isotope tracers must account for the perturbation of each isotope ratio by the presence of the other tracers.