The advantages of isothermal titration calorimetry over the other commonly used techniques, apart from giving the entire set of thermodynamic parameters, are that it is more general and suited for a wide range of molecules. It is not necessary to have compounds with chromophores or UV-visible functional groups in order to monitor the binding process as the heat signal is a universal property of binding reactions. At the same time, the signal-to-noise ratio is pretty favorable which allows for more accurate determination of the binding constants, even under very dilute conditions.