In 2005, he helped the Impact play a 15-game streak without a loss (10-0-5), setting a new league record. He was the Impact's leading scorer in 2005, following a production of nine goals and three assists for a total of 21 points. Biello also was a finalist for League MVP honors, losing to Jason Jordan, and for the fourth time in his career he was awarded the Giuseppe-Saputo Trophy. Mauro also helped the Impact capture the Voyageurs Cup for a fourth straight year. On December 7, 2005 Biello signed a two-year deal with the Impact.