Fluorescence analysis of the dissolved organic matter from soil amended with biochar revealed that biochar application increased a humic-like fluorescent component, likely associated with biochar-carbon in solution. The combined spectroscopy-microscopy approach revealed the accumulation of aromatic-carbon in discrete spots in the solid-phase of microaggregates and its co-localization with clay minerals for soil amended with raw residue or biochar. The co-localization of aromatic-C:polysaccharides-C was consistently reduced upon biochar application. These finding suggested that reduced C metabolism is an important mechanism for C stabilization in biochar-amended soils (Hernandez-Soriano "et al", 2016)