From 1985 to 1994, an "Apios" breeding program took place that resulted in the collection of over two hundred wild accessions. These accessions underwent hybridization and selection, and over 2,200 lines were assessed. Of these lines, only 53 genotypes were kept for further analysis. Three different locations and three different growing conditions—field, pots, and grow-bags— were used. There was significant variation found among almost all of the 20 genotypes in the field growing condition. Inter-node length, plant vigor, and stem diameter during plant growth were positively correlated with the plant yield below ground. There were four distinct genotypic clusters found in this collection of "Apios" lines. Several genotypes yielded large plants in all locations, maxing up to of below ground tuber. This suggests that the plant has a good ability to adapt and grow in a wide variety of locations and conditions. Furthermore, the superior germplasm identified in this project may be suitable as cultivars, and will aid in further development of "Apios" lines as a crop.