The route intersects several long-distance highways, including NY 13 in Dryden, U.S. Route 20 (US 20) and NY 5 in Auburn, and NY 31 in Port Byron. It passes over the New York State Thruway (Interstate 90 or I-90) north of Port Byron; however, there is no connection between the two. NY 38 has two suffixed routes. The first, NY 38A is an alternate route of NY 38 between Moravia and Auburn, while the other, NY 38B, is a simple east–west connector in the Southern Tier. While NY 38 runs along the western shore of Owasco Lake, NY 38A travels to Auburn along a routing east of the lake.