The destroyer transited the Panama Canal on 17 June 1942 and entered Trujillo Bay, Honduras, on 21 June with Commander Patrol Squadron 3 embarked to direct operations of the squadron on special patrols in conjunction with other naval units attempting to locate Axis submarine bases. After being contacted by patrol planes, a party from "Goldsborough" boarded Honduran merchant ship "Laguna" on 25 June and the Honduran merchant ship "Racer" the following day. Both were turned over to British authorities at Belize. On 3 July "Goldsborough" departed Puerta Castilla for Portland Bight, Jamaica. Here she tended aircraft on special patrols in the protection of convoys between Cuba and the Panama Canal. She arrived at the Charleston Navy Yard from Jamaica 2 October 1942 for repairs, followed by gunnery practice in the Chesapeake Bay.