Bioethanol plants and sugarcane mills are well-established processes where the biorefinery concept can be implemented since sugarcane bagasse is a feasible feedstock to produce fuels and chemicals; lignocellulosic bioethanol (2G) is produced in Brazil in two plants with capacities of 40 and 84 Ml/y (about 0.4% of the production capacity in Brazil). TEA of ethanol production using mild liquefaction of bagasse plus simultaneous saccharification and co-fermentation shows a minimum selling price between 50.38 and 62.72 US cents/L which is comparable with the market price. The production of xylitol, citric acid and glutamic acid from sugarcane lignocellulose (bagasse and harvesting residues), each in combination with electricity have been evaluated; the three biorefinery systems were simulated to be annexed to an existing sugar mill in South Africa. The production of xylitol and glutamic acid has shown economic feasibility with an Internal Rate of Return (IRR) of 12.3% and 31.5%, exceeding the IRR of the base case (10.3%). Likewise, the production of ethanol, lactic acid or methanol and ethanol-lactic acid from sugarcane bagasse have been studied; lactic acid demonstrated to be economically attractive by showing the greatest net present value (M$ 476–1278); in the same way; the production of ethanol and lactic acid as co-product was found to be a favorable scenario (net present value between M$ 165 and M$ 718) since this acid has applications in the pharmaceutical, cosmetic, chemical and food industry.