In 2016, two studies reported that limb loss in snakes is associated with DNA mutations in the Zone of Polarizing Activity Regulatory Sequence (ZRS), a regulatory region of the sonic hedgehog gene which is critically required for limb development. More advanced snakes have no remnants of limbs, but basal snakes such as pythons and boas do have traces of highly reduced, vestigial hind limbs. Python embryos even have fully developed hind limb buds, but their later development is stopped by the DNA mutations in the ZRS.