The castle was the scene of the marriage and wedding festivities, on 4 January 1562, of Patrick's daughter Jean (d. before July 1599) and John Stewart, Prior of Coldingham and illegitimate son of King James V. John Stewart's half-sister Mary, Queen of Scots, spent a few nights at the castle while attending this wedding. The Earl of Bothwell was implicated in February 1567 in the murder of Queen Mary's husband Henry Stuart, Lord Darnley, and became Mary's third husband in May of that year. In December, all Bothwell's titles and estates, including Crichton, were forfeited.