The movement of calcium, potassium, and chloride ions in pulvini cells has been analyzed to better understand how ion and water flux affect "M. pudica" leaves drooping. A batch of "M. pudica" were grown and watered daily, and 10-20 pulvini were collected from each group of pulvini reactive to touch, and pulvini unreactive to touch. To further understand the movement of the ions, the upper and lower halves of all collected pulvini underwent separate ion analysis using the x-ray fluorescence spectroscopy method. This method tracked the location of the ions by coloring them each with a different color of fluorescence dye. In terms of calcium concentrations, there was a significant difference in concentration between both halves of the pulvini, in both the reactive and nonreactive pulvini. For potassium, higher concentrations were found throughout the reactive pulvini, while a high concentration difference in the top and bottom halves of the unreactive pulvini was found.