Comparing to other expression systems, such as S2-cells from "Drosophila melanogaster" and Chinese hamster ovary cells, "Pichia" usually gives much better yields.Generally, cell lines from multicellular organisms require complex and expensive types of media, including amino acids, vitamins, as well as other growth factors. These types of media significantly increase the cost of producing heterologous proteins. Additionally, since "Pichia" can grow in media containing only one carbon source and one nitrogen source, which is suitable for isotopic labelling applications, like protein NMR.