The section of Route 3 between Brighton Road (Stop 35) and East Malvern (Darling Road) was constructed by the Prahran & Malvern Tramways Trust (PMTT). Initially, a line was opened from St Kilda Beach to Hawthorn Road along Carlisle Street/Balaclava Road on 12 April 1913. This line was extended east by the PMTT from Hawthorn Road to Darling Road, Malvern East on 13 November 1913. Meanwhile, the Melbourne, Brunswick & Coburg Tramways Trust (MBCTT) constructed the section of Route 3 north of Queensberry Street towards Melbourne University. This section opened on 31 October 1916.