Tuppy's next career step was the Carlsberg Laboratory in Copenhagen, from where he returned to the University of Vienna in 1951 to become an assistant at the Institute for Chemistry II. In 1956 he completed the "Habilitation", the highest academic qualification in the Austrian academic system which qualifies the recipient to supervise doctoral students and, ultimately, to hold senior faculty positions. In 1963, Tuppy became Professor of Biochemistry in the Institute of Biochemistry at the University of Vienna. In 1973, he received the Schrödinger Prize of the Austrian Academy of Sciences.