By the sixth century BC, the tribes living in the southern Colchis (Macrones, Mossynoeci, Marres etc.) were incorporated into the nineteenth satrapy of Persia. The Achaemenid Empire was defeated by Alexander the Great, however following the Alexander's death a number of separate kingdoms were established in Anatolia, including Pontus, in the corner of the southern Black Sea, ruled by the Persian nobleman Mithridates I. Culturally, the kingdom was Hellenized, with Greek as the official language. Mithridates VI conquered the Colchis, and gave it to his son Mithridates of Colchis.