The Reading to Taunton branch of the Great Western Mainline railway bisects Southcote laterally. At the eastern boundary of the settlement is Southcote Junction, where the Reading to Taunton and Reading to Basingstoke Lines diverge. A third line—the Coley branch line—ran from the junction until its closure in the 1980s. There are a number of Brunel-designed brick arch bridges along the Reading to Taunton line through Southcote, two of which have been described as "notably well preserved examples" and are designated "heritage assets" by Network Rail. A third bridge, which carries the main Burghfield to Reading road, may have originally been timber before being replaced with a steel deck by the Great Western Railway company.