The second flight took place on March 4. Semprini (according to what he later recalled) accelerated the aircraft to 100 or 110 km/h (54–59 kn, 62–68 mph), pulling the yoke toward himself; suddenly the Transaereo took off and started climbing in a sharp nose-up attitude; the pilot reduced the throttle, but then the aircraft's tail started falling and the aircraft lost altitude, out of control. The tail soon hit the water and was rapidly followed by the nose of the aircraft, which slammed into the surface, breaking the fore part of the hull. The fore wing set collapsed in the water together with the nose of the aircraft, while the central and the aft wing sets, together with the tail of the aircraft, kept floating. The pilot and the flight engineers escaped the wreck unscathed.