During his surprise appearance at a Kid Sister concert in New York's Museum of Natural History on January 25, 2008, West delivered a performance of the song. West performed "Can't Tell Me Nothing" as a duet with collaborator Jay-Z at the American Airlines Arena in Miami for the opening concert of the Heart of the City Tour, co-headlined by Mary J. Blige and Jay-Z, on March 22, 2008. At the opening of Murakami's exhibit in the Brooklyn Museum on April 4 of that year, West performed the song live, extending the performance into a freestyle. West and Young Jeezy performed "Can't Tell Me Nothing" together at the Philips Arena in Atlanta for the 13th annual Hot 107.9 Birthday Bash concert on June 13, 2008. During the first concert of West's conceptual Glow in the Dark Tour, an LCD screen seated behind him displayed a fiery explosion that ignited for the song's "oh" vocals while he performed. West performed the song on the final night of Lollapalooza 2008 in his hometown of Chicago, which he co-headlined with Nine Inch Nails. Dressed in an all red outfit, West performed "Can't Tell Me Nothing" during his set at Z100's the Jingle Ball concert in Madison Square Garden on December 12, 2008.