According to the City of Waterloo, Ontario, the indigenous people who lived in the area in the pre-historic era included the Neutral Nation. In 2020, a site in nearby Kitchener, Ontario was found to include artifacts from an Iroquioan village that was inhabited circa 1300 to 1600. Archeologists found some 35,000 objects including stone tools and a 4,000 year old arrowhead. Another source states that the site "is among a cluster of Attawandaron villages in this part of the region". n 1976, a Neutral Nation cemetery was unearthed in Grimsby, Ontario. The area that now comprises Morriston in Puslinch, Ontario is said to have been inhabited by the Neutral Nation, in a village of 4,000. This region may have had the largest Neutral Nation settlement in Ontario, at one time.