The bus station consists of three stands (numbered A1–A3) located in a bus-only lay-by on the northern side of the eastbound carriageway of Greens Way, a single stand (numbered B1) at a right angle to these accessed from John Street, and three stands (numbered HS1–HS3) situated a short distance away at the side of Hartley Street. The three sets of stands are in close proximity, linked by car parks and pathways. The majority of bus routes traverse Mexborough town centre on a one-way loop, with the Greens Way stops serving routes heading generally eastbound towards Doncaster and the Hartley Street stops serving westbound services towards Barnsley and Rotherham.