Haplogroup N-P43 also has been observed with very high frequency (26/29 = 89.7% of a sample from the settlement of Topanov and 19/22 = 86.4% of a sample from the settlement of Malyi Spirin) in samples of Kachins, a Turkic-speaking ethnic group or territorial subgroup of the Khakas people, from Shirinsky District of northern Khakassia. There appears to be a cline through the Sagai (another Turkic-speaking ethnic group that is now considered to be a constituent of the Khakas people), with 46.2% (55/119) of Sagai sampled from Ust’–Es’, Esino, Ust’–Chul’, and Kyzlas settlements of Askizsky District of central Khakassia belonging to haplogroup N-P43 "vs." only 13.6% (11/81) of Sagai sampled from Matur, Anchul’, Bol’shaya Seya, and Butrakhty settlements of Tashtypsky District of southern Khakassia belonging to this haplogroup. However, other researchers' samples of Khakas people have exhibited only moderate frequencies of N-P43 or potential N-P43. Derenko "et al." (2006) examined a sample of Khakassians ("n"=53) collected in the settlements of Askiz, Shirinsk, Beisk and Ordzhonikidzevsk districts of Khakass Republic and found that 15 of them (28.3%) belonged to N-LLY22g(xTat). Rootsi "et al." (2007) examined a sample of Khakas ("n"=181) and found that 31 of them (17.1%) belonged to N-P43; Ilumae "et al." (2016) retested 174 of the individuals in this sample and found that 27 of them (15.5%) belonged to the N-B478 (Asian/northern Samoyedic) subclade of N-P43 and 2 of them (1.1%) belonged to the N-L1419 (European/Volga Finnic and Chuvash) subclade of N-P43 for a total of 29 (16.7%) N-P43.