The next higher partials—B4 (a major second higher), C5 (a major second higher), D5 (a major second higher)—do not require much adjustment for even-tempered intonation, but E5 (a minor second higher) is almost exactly a quarter tone higher than it would be in twelve-tone equal temperament. E5 and F5 (a major second higher) at the next partial are very high notes; a very skilled player with a highly developed facial musculature and diaphragm can go even higher to G5, A5, B5 and beyond.