In early 1610, Duke Vincenzo negotiated a marriage between his 12-year-old daughter and Marcantonio IV Colonna, Duke and Prince of Paliano, and in March of that year a contract was signed under which a dowry was stipulated for Eleonora of 130,000 "scudi". However, the negotiations quickly ended because of a previous marriage agreement of the Colonnas with the Doria family. Then, negotiations began for the marriage of Eleonora with Victor Amadeus, Prince of Piedmont and heir of the Duchy of Savoy; however, the intended groom expected a large territorial gain as a dowry, which the House of Gonzaga refused. Duke Vincenzo died in 1612, before he could arrange his youngest daughter's marriage.