When about seventeen, Scot entered Hart Hall, Oxford, but left the university without a degree. His writings show some knowledge of law, but he is not known to have joined any inn of court. Marrying in 1568, he seems to have spent the rest of his life in his native county. His time was mainly passed as an active country gentleman, managing property which he inherited from his kinsfolk about Smeeth and Brabourne, or directing the business affairs of his first cousin, Sir Thomas Scot, who proved a generous patron, and in whose house of Scots Hall he often stayed. He was collector of subsidies for the lathe (county subdivision) of Shepway in 1586 and 1587, and he was perhaps the Reginald Scot who acted in 1588 as a captain of untrained foot-soldiers at the county muster. He was returned to the parliament of 1588–89 as member for New Romney, and he was probably a justice of the peace. He describes himself as "esquire" in the title-page of his "Discoverie", and is elsewhere designated "armiger".