During the first six weeks of 1968, "Virgo" loaded ammunition in preparation for and participation in the 1st Fleet exercise, Operation "Bead Stringer." In mid-February, she loaded ammunition for her second deployment to the western Pacific during the Vietnam War. On 26 February, she began her voyage west. The ship changed operational control to the 7th Fleet on 7 March and arrived in Subic Bay 12 days later. Once again, her assignment fell into a pattern of replenishment voyages to the ships operating in the Gulf of Tonkin. In six months' time, she made eight line swings from Subic Bay to the Gulf bringing in new stocks of ammunition to refill the depleted magazines of American warships along the Vietnamese coast. She finished her last such mission early in October and returned to Subic Bay on the 10th. From there, she moved to Sasebo, Japan, for a four-day liberty call before getting underway for the United States on 23 October. The ammunition ship arrived back in Concord on 11 November, offloaded ammunition, and entered the Mare Island Naval Shipyard to begin post-deployment standdown. On 19 December, she moved to the Triple "A" shipyard to begin a six-week restricted availability.