Like other walnuts, the roots, inner bark, nut husks, and leaves contain a nontoxic chemical called hydrojuglone, when exposed to air or soil compounds it is oxidized into juglone that is biologically active and acts as a respiratory inhibitor to some plants. Juglone is poorly soluble in water and does not move far in the soil and will stay most concentrated in the soil directly beneath the tree. Even after a tree is removed the soil where the roots once were will still contain juglone for several years after the tree is removed as more juglone will be released as the roots decay. Well drained and aerated soils will host a healthy community of soil microbes and these microbes will help to break down the juglone.