Highway 416 begins at an interchange with Highway 401, branching to the north near the community of Johnstown in the United Counties of Leeds and Grenville. This interchange only provides access to and from the west of Highway 401, but immediately north of it, a second interchange with the remaining section of Highway 16 provides access from Johnstown and to a parclo interchange with both directions of Highway 401, as well as to the Ogdensburg-Prescott International Bridge crossing to Ogdensburg, New York.