The road passes to the northwest of the university campus as it enters the city of West Haven. Here, I-95 passes mixed areas of suburban development, where it comes to an interchange with Route 162. Thru trucks to Downtown West Haven must use this exit, as trucks may not use the northbound exit ramp at the next interchange, for Campbell Avenue and the southern terminus of Route 122, due to its steep incline. The road makes another reverse turn, where it crosses the New Haven Line and the West River, and comes into the city of New Haven. Immediately after crossing the river, I-95 comes to an interchange with the southern terminus of Route 10, which provides access to the Yale Bowl and connects to Route 34. From this point, the freeway heads northeast, passing the New Haven Harbor in the neighborhood of Long Wharf, before reaching the large interchange complex southeast of Downtown New Haven. The complex includes an interchange with a flyover ramp to M.L.K. Jr. Boulevard serving the downtown area, as well as the highway's split with I-91, which heads north-northeast from I-95 towards Hartford. Past I-91, the freeway turns southeast to cross the Quinnipiac River on the Pearl Harbor Memorial Bridge, locally known as simply the "Q Bridge". On the other side of the bridge, the road heads into another area of mixed development in The Annex neighborhood. Here, I-95, expands to four lanes in the southbound direction, and has an interchange with the northern terminus of Route 337, that also provides access to Tweed New Haven Airport.