It was previously believed that Ec-MscS exhibits complex adaptive behavior, while Ec-MscL does not. A recent study showed that both Ec-MscS and Ec-MscL are capable of adaptive behavior under constant pressure stimuli in excised membrane patch; however, both mechanosensitive channels lose the adaptive ability in whole cell recordings, indicating that the previously known adaptive behavior of Ec-MscS is related to stress relaxation of the membrane instead of specific channel structure. This result further emphasizes the importance of protein-membrane interaction for mechanosensitive channels.