The regiment was again mustered in for three months' service on May 25, 1862. It left New York City for Baltimore, Maryland, on May 26, and then was attached to Dix's Command, Middle Department, to July 1862. Then, it was assigned to the 8th Army Corps, Middle Department, to September 1862. It was in camp at Stewart's Hill, Baltimore, from May 28 to June 5, and duty at Fort Federal Hill from June 6 to August 28. The regiment mustered out in New York City on September 5, 1862.