The painting depicts the moment that Jane, blindfolded, is being assisted to lay her head upon the block for the executioner. Her outstretched hand reaches uncertainly down to find the block. She is being assisted by a man who is identified as John Brydges, 1st Baron Chandos. Chandos was a Lieutenant of the Tower at the time of Jane's execution. While imprisoned in the Tower, Jane was attended by ladies in waiting, one of whom was the nursemaid of her infancy. Two ladies in waiting are depicted in the painting, showing their grief at the event which is about to take place.