A few months after the events of the first film, Professor Roy Curien at the fictional Cuesta Verde University (CVU) in Seattle, has managed to subdue and contain a "Hyper sapiens" specimen, who is revealed to be his son, Rudy Curien (a survivor of Isla del Morte incident from the first film). Curien experiments on another survivor (Alicia), trying to determine the source of her immortality, with apparent disregard for the rotted state of Alicia's body. When Curien has created a serum that he believes will bring back the dead and grant immortality, Curien murders one of his students and injects her with the serum. She returns to life, infects Curien and breaks out of the building.