Migration of living animals and settling particle-attached organisms can lead to an uneven distributions of biota at different locations of the world. When small organisms escape from a ballast tank, the foreign organism or animal can upset the balance of the local habitat and potentially damage the existing animal life. Vessel workers check the ballast tank for living organisms ≥50 μm in discrete segments of the drain, it also represents the level of sedimentary of different rock or soil in the tank. Throughout the sample collection, concentrations of organisms and marine life varied in result in the drain segments, patterns also varied in level of stratification in other trials. The best sampling strategy for stratified tanks is to collect various time-integrated samples spaced evenly throughout each discharge.