Ankiel returned to the majors in 2001 but again had issues controlling his pitches, walking 25 batters and throwing five wild pitches in 24 innings, and was sent down to Triple-A. His problems in the minors became dramatic. In innings, Ankiel walked 17 batters and threw 12 wild pitches, accumulating a 20.77 ERA. He was demoted all the way down to the Rookie League Johnson City Cardinals, where he was successful as both a starting pitcher and a part-time designated hitter (sporting a .638 slugging percentage with 10 home runs and 35 RBIs in 105 at-bats). He was voted Rookie Level Player of the Year, Appalachian League All-Star left-handed pitcher, Rookie League All-Star starting pitcher, Appalachian League Pitcher of the Year, and Appalachian League All-Star designated hitter.