In 1517, Bi'ina, with the rest of Palestine, was incorporated into the Ottoman Empire after it was captured from the Mamluks, and by 1596 appeared in tax registers as belonging to the "Nahiya" of Akka, part of Safad Sanjak. It had a population of 61 households; 46 Muslim and 15 Christian. The villagers paid a fixed tax rate of 25% on various agricultural products, including wheat, barley, olives, cotton, goats or beehives, in addition to for a press for grapes or olives; a total of 7,134 akçe.