In 1606, Louis joined the expedition, now located at Port-Royal. As a pharmacist, he was interested in plants and enjoyed horticulture, seeming to possess a "green thumb", growing hemp and other plants. He was highly regarded, and particular note was made of his knowledge and pleasure in cultivating the land. He participated in the construction of a grist-mill on the Allain River near present-day Annapolis Royal. Experimental farming activities were conducted, with various grains seeded in the local fields. He looked after the health of the pioneers, and cultivated native drug plants introduced to him by the Micmac Indians. He returned to France in 1607, after the trade concession that had been granted to the de Monts expedition had expired.