Buckstone was first married in 1828 to Anne Maria Honeyman, with whom he had at least five children before she died in 1844. For many years, Buckstone was closely associated with the actress Fanny Copeland Fitzwilliam, who was widowed in 1852 and whom he was engaged to marry in 1854. She died of cholera a month before the wedding. In 1857 Buckstone married Fanny's cousin Isabella Copeland, the great-niece of the theatre manager Robert Copeland, and they had 12 children between 1857 and 1876. Their daughter, Lucy Isabella Buckstone and their sons John Copeland Buckstone and Rowland Buckstone also took to the stage.