Chambers' best game of the season (and his career) came on December 4 against the Buffalo Bills. In the game, which the Dolphins trailed by 20 points entering the fourth quarter, Chambers caught 15 passes for 238 yards and a touchdown. Key receptions included a 57-yard grab on the final drive with under two minutes left, as well as the game-winning touchdown on a pass from Sage Rosenfels with only six seconds remaining. His impressive performance earned him AFC Offensive Player of the Week honors. His yardage total was the highest for any player in the NFL in 2005, and the most by any player since Plaxico Burress had 253 yards for the Pittsburgh Steelers in 2002.