In music theory, a comma is a very small interval, the difference resulting from tuning one note two different ways. The word "comma" used without qualification refers to the syntonic comma, which can be defined, for instance, as the difference between an F tuned using the D-based Pythagorean tuning system, and another F tuned using the D-based quarter-comma meantone tuning system. Intervals separated by the ratio 81:80 are considered the same note because the 12-note Western chromatic scale does not distinguish Pythagorean intervals from 5-limit intervals in its notation. Other intervals are considered commas because of the enharmonic equivalences of a tuning system. For example, in 53TET, B and A are both approximated by the same interval although they are a septimal kleisma apart.