Madame Web again returns in a back-up feature in "The Amazing Spider-Man" #600. She looks into the future, showing what are apparently quick looks into Spider-Man's future, only to see someone "unravelling the web of fate", and fearfully exclaiming "They're hunting spiders." After that, she is attacked by Ana Kravinoff and her mother, Sasha. The pair incapacitate her and then claim "we now have our eyes". She is seen still captured by Ana and her mother, as they inspect their new quarry, Mattie Franklin. While still bound in a chair, she apologizes to a then-unconscious Mattie, who is later killed by Sasha Kravinoff.