The CESNET e-infrastructure includes institutions such as public, state and private universities, institutes of the Academy of Sciences of the Czech Republic, university hospitals, scientific and other libraries, technological and innovation centres and science parks, important cultural institutions and many other scientific and research institutions. The total number of individual users with access to the CESNET e-infrastructure in the Czech Republic is about 450 thousand persons. Thanks to a direct connection with GÉANT, the backbone network for science, research and education, and also thanks to a connection to research networks in other world regions provided by GÉANT (Internet2 and ESnet in the USA, AfricaConnect in Africa, TEIN in Asia and CLARA in the Pacific and Latin America), the CESNET e- infrastructure even allows cooperation and operation of demanding and unique applications and data sharing in the multinational and international scientific community. The CESNET e-infrastructure is included in the Czech Roadmap showing large infrastructures used for research, experimental development and innovation of the Ministry of Education, Youth and Sport of the Czech Republic.