Chambers broke the ten second barrier twice in the 2001 World Championships with a personal best of 9.97 s in the quarter finals and a run of 9.99 s in the final. This was not enough for a medal in a strong race which featured five sprinters running under ten seconds and saw Greene defend his title. Chambers was later upgraded to fourth place after silver medallist Tim Montgomery tested positive for banned substances. A hamstring injury in the final ruled him out of the 200 m event. Chambers ended his season at the 2001 Goodwill Games in Brisbane, and finished in first place in the absence of an injured Greene, who commentated on his European rival's victory.