Just south of its partial cloverleaf interchange with SR 659 (Pughsville Road), I-664 crosses a rail line; a spur from that rail line heads north in the median of the freeway as the highway enters the city of Suffolk. The rail spur leaves the median and heads northeast toward Portsmouth just south of its interchange with SR 164 (Western Freeway) and US 17 (Bridge Road). SR 164 heads east toward downtown Portsmouth while US 17 heads northwest to the James River Bridge. There is no access from southbound I-664 to southbound US 17; that movement is made via the next interchange, a cloverleaf interchange with SR 135 (College Drive) that serves satellite campuses of Tidewater Community College and Old Dominion University and the Portsmouth neighborhood of Churchland.