Chambers topped the football team in receiving his sophomore season when he totaled 28 catches for 563 yards and seven touchdowns, including an 80-yard grab against Michigan. He missed two games as a junior with a broken finger, but still led the team in receiving with 41 catches for 578 yards and four scores, and was an honorable mention All-Big Ten selection. Despite playing in only nine games as a senior because of a stress fracture in his right foot, Chambers put together the most productive season of his career with 52 receptions for 813 yards and five touchdowns. He was a second-team All-Big Ten Conference selection. He had the best game of his career when he hauled in 11 passes for 191 yards against Iowa. That game came one week after he had 11 receptions for 173 yards and a touchdown against Purdue.