Chambers was drafted by the Miami Dolphins 52nd overall in the second round of the 2001 NFL Draft. Chambers turned in one of the most productive seasons of any rookie wide receiver in club history. He started seven of the 16 games in which he played, and finished with 48 receptions for 883 yards and seven touchdowns. His yardage and touchdown totals both led the team, as did his 18.4-yards per reception average. His reception figure ranked third on the team behind those of fellow receivers Oronde Gadsden and James McKnight. Chambers finished the year ranked 18th in the AFC in receiving yards, while his average per catch was first among the top 20 receivers in both the AFC and NFC. Chambers topped the 100-yard receiving mark three times. He also returned 36 kickoffs for a 22.5-yard average with a long of 47. Chambers missed the team's first-round playoff game on January 13, 2002 against the Baltimore Ravens due to a sprained ankle suffered the last week of the regular season.