Chambers suffered a down season in 2006 after a Pro Bowl performance the year before. The Dolphins' offense struggled as a whole, and he never seemed to be a favorite target for quarterback Joey Harrington, and finished third on the team in both receptions and receiving yards. He also ranked third on the team in rushing with eight rushes for 95 yards. Chambers led the team in receptions four times and in reception yardage four times during a season in which he started all 16 games. He was held without a catch on December 17 at the Buffalo Bills, breaking his streak of making at least one reception in 60 consecutive games.