In 1528, Margaret Tudor, now Regent of Scotland for her infant son James V, married Henry Stewart, 1st Lord Methven, a descendant of Albany. His brother, Sir James Stewart (c. 1513–1554), was made Captain of Doune Castle, and Sir James' son, also James (c. 1529–1590), was created Lord Doune in 1570. Lord Doune's son, another James (c. 1565–1592), married Elizabeth Stuart, 2nd Countess of Moray around 1580, becoming Earl of Moray himself. The castle thus came to be the seat of its keepers, the Earls of Moray, who owned it until the 20th century.