Once settled, there was frequent conflict, both with Spanish-allied Indians and with the English at Charles Town, the latter over English attempts to assert authority over the Scots and rights to the lucrative Indian trade. The Scots also carried out frequent raids on Spanish allied Indians and raided the Spanish mission at Santa Catalina de Guale as well as encouraging (and arming) their Yamasee trading partners to attack the Spanish directly. In August 1686, the Spanish retaliated and sent three ships with 150 Spanish troops and Indian allies to attack Stuarts Town. Due to a recent sickness, the Scots had only 25 effective fighting men able to mount a defence and the town was wiped out. There was no retaliation by the English, who were warned by the Proprietors not to interfere.