Sometime between 1666 and 1671, Nicholas Greenberry married Anne (surname unknown). Nicholas and Anne Greenberry had at least four children, two born in England: Charles, born in 1672 at Holburn, London, Middlesex, England and Katherine, born in 1674 at Holburn, London, Middlesex, England, and two born in Maryland: Anne, born in 1676 and Elizabeth, born in 1678. English burial records show two children of a Nicholas Greenberry were buried in England between 1667 and 1671: Anne, buried 6 Aug 1667 at St Giles, Goudhurst, Kent, England, and Elizabeth, buried 19 Mar 1671 at Hoborn, London, Middlesex, England.