After launch from Cape Canaveral Air Force Station Launch Complex 12, the protective shroud covering Mariner 4 was jettisoned and the Agena-D/Mariner 4 combination separated from the Atlas-D booster at 14:27:23 UTC on November 28, 1964. The Agena's first burn took place from 14:28:14 to 14:30:38. The initial burn put the spacecraft into an Earth parking orbit and the second burn from 15:02:53 to 15:04:28 injected the craft into a Mars transfer orbit. Mariner 4 separated from the Agena at 15:07:09 and began cruise mode operations. The solar panels deployed and the scan platform was unlatched at 15:15:00. Sun acquisition occurred 16 minutes later.