The "P. fluorescens" relaxed evolutionary group that was defined in, on the basis of the genus phylogenomic tree, comprised 96 genomes and displayed high levels of phylogenetic heterogeneity. It comprised many species, such as "Pseudomonas corrugata, Pseudomonas brassicacearum, Pseudomonas frederiksbergensis, Pseudomonas mandelii, Pseudomonas kribbensis, Pseudomonas koreensis, Pseudomonas mucidolens, Pseudomonas veronii, Pseudomonas antarctica, Pseudomonas azotoformans, Pseudomonas trivialis, Pseudomonas lurida, Pseudomonas azotoformans, Pseudomonas poae, Pseudomonas libanensis, Pseudomonas synxantha", and "Pseudomonas orientalis". The core proteome of the "P. fluorescens" group comprised 1396 proteins. The protein count and GC content of the strains of the "P. fluorescens" group ranged between 4152 and 6678 (average: 5603) and between 58.7–62% (average: 60.3%), respectively. Another comparative genomic analysis of 71 "P. fluorescens" genomes identified eight major subgroups and developed a set of nine genes as markers for classification within this lineage.