Alstott was selected in the second round (35th overall) by the Tampa Bay Buccaneers. The Buccaneers utilized Alstott primarily as a running fullback as opposed to a blocking fullback that teams were starting to switch to at the time. He was a member of the Tampa Bay Buccaneers team that won Super Bowl XXXVII (2002), played at Qualcomm Stadium in San Diego, California. Alstott rushed for 15 yards and the first Super Bowl touchdown in Tampa Bay Buccaneers history. He also caught five passes for 43 yards.