"Of Girls and Horses", focuses on the relationship that develops between 16-year-old high school dropout Alex and upper-class Kathy at a horse farm. Alex works at the horse farm for a school internship, supervised by lesbian riding instructor Nina. In an interview from the website Afterellen, conducted by Marcie Bianco, Treut reveals her intentions for the movie and the message she hopes it will send. She says, "In this film, I just wanted to picture insecurities that queer teenagers face and find a way of coming out of it...".