At Wimbledon, Querrey faced Novak Djokovic in the 3rd round while his opponent was holding all four majors. Despite being listed as high as a 30-1 underdog, Querrey upset the world No. 1 player in four sets to snap Djokovic's streak of reaching at least the quarterfinals in every Grand Slam since the 2009 French Open, and his streak of 30 consecutive Grand Slam match wins. It was also Querrey's first win over a No. 1 ranked player. He then continued his successful run by defeating Nicolas Mahut to become the first American man to reach the quarterfinals of a Grand Slam since John Isner and Andy Roddick did so at the 2011 U.S. Open. In the quarterfinals, he lost to Canadian Milos Raonic in four sets. Despite this defeat, the 2016 Championships marked Querrey's best ever performance at a Grand Slam.