In August 1990, as DRF 1 for the 82nd Airborne Division, the 4/325 was called on to spearhead the deployment of U.S. forces to the Persian Gulf in response to the Iraqi Invasion of Kuwait. Their mission was to secure the area north of the approach to Al Jubayl, Arabia and keep secure the approach to Al Jubayl until US Marine Forces arrived later in August. While reinforcements streamed into the country, the 325th along with the remainder of the 82nd Airborne Division conducted the most intensive combat trainup in the unit's history.