The Guaíra Falls was an effective barrier that separated freshwater species in the upper Paraná basin (with its many endemics) from species found below it, and the two are recognized as different ecoregions. After the falls disappeared, many species formerly restricted to one of these areas have been able to invade the other, causing problems typically associated with introduced species. For example, more than 30 fish species that formerly were restricted to the region below the falls have been able to invade the region above.