Within the same tuning system, two enharmonically equivalent notes (such as G and A) may have a slightly different frequency, and the interval between them is a comma. For example, in extended scales produced with five-limit tuning an A tuned as a major third below C5 and a G tuned as two major thirds above C4 are not exactly the same note, as they would be in equal temperament. The interval between those notes, the diesis, is an easily audible comma (its size is more than 40% of a semitone).