During artificial insemination, semen is most frequently deposited intra-vaginally by means of a plastic syringe. In order for semen to be deposited here, the vaginal orifice is everted through the cloaca. This is simply done by applying pressure to the abdomen of the hen. The semen-containing instrument is placed 2–4 cm into the vaginal orifice. As the semen is being deposited, the pressure applied to the hen's abdomen is being released simultaneously. The person performing this procedure typically uses one hand to move and direct the tail feathers, while using the other hand to insert the instrument and semen into the vagina.