Niederhoffer studied statistics and economics at Harvard University (B.A. 1964) and the University of Chicago (Ph.D. 1969). He was a finance professor at the University of California, Berkeley (1967–1972). In 1965, while still at college, he co-founded with Frank Cross a company called Niederhoffer, Cross and Zeckhauser, Inc., an investment bank which sold privately held firms to public companies. This firm is now called Niederhoffer Henkel, and was run by Lee Henkel (who died May 30, 2008), the former general counsel to the IRS. Niederhoffer pioneered a mass marketing approach in investment banking and did a large volume of small deals at this firm. He also bought many privately held firms with Dan Grossman, his partner during this period.