Adrienne von Speyr was born in La Chaux-de-Fonds, Switzerland, to an upper-middle-class Protestant family. Her father, Theodor von Speyr, was an ophthalmologist. Her mother, Laure Girard, was the descendant of a family of noted watchmakers and jewelers from Geneva and Neuchâtel. Adrienne was her parents' second child. Her sister, Hélène, was a year and a half older. Her first brother, Willy, who became a physician, was born in 1905 and died in 1978. Her second brother, Theodor, was born in 1913 and served as the director of the Swiss Bank Corporation in London for many years.