In 1928, PA 41 was rerouted at Lancaster to head southeast to the Delaware border near Kaolin. The new alignment ran along US 222/PA 72 from Lancaster south and east to Lampeter, where PA 41 headed east through Strasburg to Gap. From Gap, PA 41 ran southeast along the Gap Newport Pike to the Delaware border. At this time, all of PA 41 was paved except for the portion between Strasburg and Gap. PA 240 replaced the PA 41 designation along the US 222 concurrency between Lancaster and Reading. By 1930, the western terminus of PA 41 was cut back to US 222 near Lampeter, removing the concurrences with US 230 and US 222, while the road from PA 41 in Gap north to US 30 was designated as PA 741. By this time, the route was paved from a point east of Strasburg east to Gap. The northern terminus of PA 41 was moved to its current location at US 30 by 1940, replacing PA 741. Meanwhile, PA 741 was designated onto the former alignment of PA 41 between US 222 near Lampeter and Gap. In 1954, PA 41 was rerouted to bypass Atglen and Christiana to the northeast along its current alignment, having previously followed Fallowfield Road, Main Street, Zion Hill Road, and Newport Avenue. By 1959, PA 41 was realigned to the east of Cochranville, having previously passed through the community on Cochran Street.