The southern terminus is at U.S. 98/301 south of Trilby. The trail goes north to a crossover of U.S. 98/SR 50, a mile east of I-75 and north past the Silver Lake Campground in the Withlacoochee State Forest, close to the Withlacoochee River. The trail runs under I-75 as it continues north and about a mile from there intersects the Good Neighbor Trail at 28°35'22.2"N 82°13'42.3"W. From there it runs through Nobleton, Istachatta, and then crosses the Hernando-Citrus County Line into Pineola, the site of the Great Train Wreck of 1956.