The play opens with Cardinal Camillo discussing with Count Francesco Cenci a murder in which Cenci is implicated. Camillo tells Cenci that the matter will be hushed up if Cenci will relinquish a third of his possessions, his property beyond the Pincian gate, to the Church. Count Cenci has sent two of his sons, Rocco and Cristofano, to Salamanca, Spain in the expectation that they will die of starvation. The Count's virtuous daughter, Beatrice, and Orsino, a prelate in love with Beatrice, discuss petitioning the Pope to relieve the Cenci family from the Count's brutal rule. Orsino withholds the petition, however, revealing himself to be disingenuous, lustful for Beatrice, and greedy. After he hears the news that his sons have been brutally killed in Salamanca, the Count holds a feast in celebration of their deaths, commanding his guests to revel with him. Cenci drinks wine which he imagines as "my children's blood" which he "did thirst to drink!" During the feast, Beatrice pleads with the guests to protect her family from her sadistic father, but the guests refuse, in fear of Cenci's brutality and retribution.