His last completed work, published in 1606, was a collection of thirty-six songs based on eight-line poems, divided into twelve groups, each of which contained three settings in each of the twelve modes. The work, "Octonaires de la vanité et inconstances du monde" ("Eight-line Poems on the Vanity and Inconstancy of the World"), based on poems by the Calvinist preacher Antoine de Chandieu, was for groups of three or four voices. According to Le Jeune's sister Cecile, who wrote the introduction to the publication, he had intended to complete another set for more voices but died before finishing it. It was one of the last collections of chansons of the Renaissance, of any type; following its publication, the "air de cour" was the predominant genre of secular song composition in France.