ABLV was confirmed in two horses on Queensland's Darling Downs in May 2013. Both horses were euthanased when their condition deteriorated despite treatment and the attending veterinarian performed a "post mortem" examination obtaining samples that allowed for the laboratory diagnosis. The property was then quarantined. Three dogs and the four horses in closest contact received postexposure prophylaxis, as did all nine in-contact people. The virus was isolated and identified as the insectivorous bat strain. These cases have prompted reconsideration of the potential spillover of ABLV into domestic animal species. Veterinarians are urged to consider ABLV as a differential diagnosis in cases of progressive generalized neurological disease.