Chalcones have been identified as the potential anti-infective candidates that inhibit various parasitic, malarial, bacterial, viral, and fungal targets like cruzain-1/2, trypanopain-Tb, trans-sialidase, glyceraldehyde-3-phosphate dehydrogenase (GAPDH), fumarate reductase, falcipain-1/2, β-hematin, topoisomerase-II, plasmepsin-II, lactate dehydrogenase, protein kinases (Pfmrk and PfPK5), sorbitol-induced hemolysis, recombinant dengue virus Type 1 (DEN-1 NS3), influenza A virus (H1N1), human immunodeficiency virus (HIV-Integrase/Protease), protein tyrosine phosphatase A/B (Ptp-A/B), filamentous temperature-sensitive mutant Z (FtsZ), fatty acid syntheses (FAS-II), lactate/isocitrate dehydrogenase, NorA efflux pump, deoxyribonucleic acid (DNA) gyrase, fatty acid synthase, chitin synthase, β-(1,3)-glucan synthase, etc.