commiss decis juli guidelin classif refer articl direct eec eec commiss european commun regard treati establish european econom commun regard council direct eec april contain genet modifi micro organ articl thereof purpos direct genet modifi micro organ classifi group ii criteria annex ii guidelin classif refer articl commiss requir establish entri forc direct eec guidelin classif provis decis receiv favour opinion committe member state repres accord procedur laid articl direct eec adopt decis articl classif genet modifi micro organ made articl direct eec annex guidelin classif interpret annex ii direct eec articl decis address member state brussel juli commiss carlo ripa di meana member commiss oj annex guidelin classif genet modifi micro organ group articl direct eec classif group guidelin interpret annex ii direct eec characterist recipi parent organ pathogen recipi parent organ classifi pathogen satisfi condit paragraph recipi parent strain establish record safeti laboratori industri advers effect human health environ ii recipi parent strain meet condit paragraph belong speci long record biolog work includ safeti lab industri show advers effect human health environ iii recipi parent organ strain satisfi condit paragraph belong speci record biolog work includ safe laboratori industri test includ anim carri order establish pathogen safeti environ iv virul strain acknowledg pathogen speci strain defici genet materi determin virul ensur revers pathogen case bacteria special attent plasmid phage born virul determin adventiti agent recipi parent strain cell line free biolog contamin agent symbiont mycoplasma virus viroid potenti harm recipi parent strain cell line proven extend histori safe built biolog barrier interf optim growth reactor fermentor confer limit surviv replic advers consequ environ applic type oper characterist vector vector character purpos characterist account inform composit construct type vector defin viru plasmid cosmid phasmid transpos element minichromosom inform constitu fragment vector origin fragment progenitor genet element strain organ progenitor genet element natur occur ii fragment synthet function method construct inform vector structur size vector express basepair function rel posit structur gene ii marker gene select antibiot resist heavi metal resist phage immun gene code degrad xenobiot iii regulatori element iv target site nic site restrict endonucleas site linker transpos element includ proviru sequenc vi gene relat transfer mobil function respect conjug transduct chromosom integr vii replicon vector free harm sequenc vector gene code potenti harm pathogen trait virul determin toxin type oper gene constitut essenti featur vector condit circumst result harm pathogen phenotyp genet modifi micro organ vector limit size genet sequenc requir perform intend function vector increas stabil genet modifi micro organ environ requir intend function vector poorli mobiliz vector plasmid restrict host rang ii defect transfer mobil factor tra mob type oper tra mob type oper vector viru cosmid phasmid restrict host rang ii render lysogen clone vector defect ci lamda repressor transfer resist marker micro organ acquir natur acquisit compromis drug control diseas agent requir characterist insert insert character purpos characterist account origin insert genu speci strain inform librari insert origin sourc method obtain nucleic acid interest cdna chromosom mitochondri ii vector librari construct lamda gt pbr site dna insert iii method identif coloni hybrid immuno blot iv strain librari construct insert synthet intend function identifi inform structur insert requir inform structur gene regulatori element ii size insert iii restrict endonucleas site flank insert iv inform transpos element proviru sequenc insert free harm sequenc function genet unit insert defin applic type oper ii insert gene code potenti pathogen trait virul determin toxin type oper gene constitut essenti part insert circumst result harm pathogen phenotyp genet modifi micro organ insert limit size genet sequenc requir perform intend function insert increas stabil construct environ requir intend function insert poorli mobiliz instanc transpos transferr proviru sequenc function transpos sequenc requir characterist genet modifi micro organ genet modifi micro organ pathogen requir assur complianc requir genet modifi micro organ safe man environ recipi parent strain applic type oper genet modifi micro organ safe reactor fermentor recipi parent strain limit surviv replic reactor fermentor advers consequ environ applic type oper genet modifi micro organ includ group meet condit construct singl prokaryot recipi includ indigen plasmid virus singl eukaryot recipi includ chloroplat mitochondria plasmid exclud virus consist genet sequenc speci exchang sequenc physiolog process