council decis novemb lai method analysi test heat treat milk direct human consumpt eec council european commun regard treati establish european econom commun regard council direct eec august health anim health problem affect intra commun trade heat treat milk articl thereof regard propos commiss pursuant articl direct eec council lai detail arrang check provid articl purpos check carri establish supervis respons offici depart period inspect offici depart ensur complianc requir direct eec laid articl thereof lai procedur check includ determin method emploi implement method prescrib determin total solid content fat content solid fat content total nitrogen content protein content densiti heat treat milk intend direct human consumpt technic reason initi requir lai refer method analysi test ensur standard prescrib articl direct eec met import make studi condit routin method analysi test pend outcom studi compet author routin method view ensur standard met determin aforement method includ determin analyt procedur fix criteria reliabl order ensur uniform interpret result adopt decis articl method analys test heat treat milk determin total solid content determin fat content determin total fat solid content determin total nitrogen content determin protein content determin specif mass articl implement refer method analysi test determin criteria reliabl collect sampl carri rule set annex articl method refer articl set annex ii articl decis address member state brussel novemb council presid gummer oj amend direct eec oj annex gener provis introduct gener provis respect reagent equip express result precis test report compet author member state enforc laboratori charg sampl test milk respect provis reagent water mention made water solut dilut rins purpos distil water deioniz water deminer water equival puriti refer made solut dilut indic solut water dilut water meant chemic chemic recogn analyt qualiti equip list equip list equip refer method item special item specif analyt balanc analyt balanc mean balanc capabl weigh mg express result result result state test report arithmet obtain test satisfi repeat criterion state method repeat criterion satisfi test repeat result declar invalid calcul percentag result calcul percentag mass sampl precis criteria repeat reproduc precis criteria method defin repeat absolut differ singl test result obtain procedur ident test materi condit oper apparatu laboratori short interv time li reproduc absolut differ singl test result obtain procedur ident test materi condit oper apparatu laboratori time li individu method valu repeat reproduc criteria procedur repres confid level interv defin iso ed collabor trial studi plan conduct accord intern guidelin test report test report method analysi result obtain addit give detail procedur method analysi option circumst influenc result obtain test report give inform complet identif sampl ii sampl heat treat milk scope field applic procedur specifi refer method sampl transport storag sampl heat treat milk gener sampl heat treat milk tank carri skill oper suitabl train undertak sampl milk compet author test laboratori instruct sampl personnel sampl techniqu ensur sampl repres conform entir batch compet author test laboratori instruct sampl personnel mark sampl ensur unambigu ident sampl sampl equip gener sampl equip made stainless steel suitabl materi adequ strength construct suitabl intend purpos mix sampl plunger agit mix liquid contain suffici area produc adequ mix product caus develop rancid flavour dipper solid handl suffici length enabl sampl depth contain obtain capac dipper ml sampl contain closur glass suitabl metal plastic materi sampl equip includ contain closur construct chang sampl affect result examin surfac sampl equip sampl contain clean dry smooth free crevic corner round sampl techniqu gener irrespect test perform milk mix prior sampl manual mechan mean sampl immedi mix milk agit volum sampl adequ test requir capac sampl contain fill complet sampl allow proper mix content test avoid churn transport manual sampl sampl divid bulk quantiti milk sampl contain repres quantiti contain note quantiti milk sampl relat sampl contain test individu mix portion repres quantiti amount proport quantiti contain sampl sampl bulk proportion amount mix sampl larg vessel storag rail road tank mix milk procedur sampl mix content larg vessel storag rail road tank mechan agit advis extent mix period time milk rest effici procedur mix appli circumst demonstr adequ purpos analysi envisag criterion mix effici influenc similar analyt result sampl part consign outlet tank interv discharg procedur mix milk untreat milk milk consid effici differ fat content sampl condit larg vessel bottom discharg outlet discharg point small quantiti milk repres content mix sampl prefer manhol sampl discharg outlet run suffici milk ensur sampl repres mix content larg vessel storag rail road tank carri mechan agit built tank driven electr motor propellor agit driven electr motor manhol agit suspend milk case rail road tanker recircul milk transfer hose attach tanker unload pump insert manhol clean filter compress air case minim air pressur volum prevent develop rancid flavour sampl heat treat milk direct consumpt retail pack sampl heat treat milk direct consumpt retail packag complet seal packag sampl packag machin cold room treatment establish process pasteur milk dai process sampl type heat treat milk pasteur uht treat steril number examin made accord instruct laid test laboratori compet author identif sampl sampl mark identif code readili identifi instruct test laboratori compet author preserv transport storag sampl accord compet nation author instruct condit preserv chemic temperatur transport storag time sampl analysi milk prepar test laboratori type milk procedur analysi instruct point includ transport storag precaut prevent exposur contamin odour direct sunlight contain sampl transpar store dark place anex ii determin total solid content scope field applic procedur specifi refer method determin total solid content milk definit total solid content mass remain complet dry procedur express percentag mass principl evapor water test portion temperatur oc dry oven apparatu glasswar usual laboratori equip analyt balanc desicc provid effici desicc freshli dri silica gel water content indic dry oven ventil thermostat control oc total work space flat bottom dish height mm diamet mm materi provid fit readili remov lid boil water bath homogen prepar test sampl bring sampl milk temperatur oc mix ensur homogen distribut fat sampl avoid agit vigor froth milk churn fat found difficult dispers cream layer warm slowli oc care mix incorpor cream adher contain cool sampl quickli oc desir homogen assist dispers fat correct result expect sampl separ liquid fat visibl separ irregularli shape white particl adher wall contain procedur prepar dish heat dish lid oven control oc minut place lid dish immedi transfer desicc cool room temperatur minut weigh nearest mg test portion immedi weigh nearest mg prepar test sampl prepar dish determin pre dry dish minut heat boil water bath heat dish lid alongsid oven control oc hour place lid dish remov oven cool desicc room temperatur minut weigh nearest mg heat dish lid alongsid oven hour place lid dish remov oven cool approxim minut desicc weigh nearest mg repeat oper differ mass consecut weigh exce mg record lowest mass express result calcul formula calcul total solid content parcentag mass wt wt total solid content mass gram dish lid mass gram dish lid test portion mass gram dish lid dri test portion round obtain nearest percentag mass precis repeat total solid product reproduc total solid product ii determin fat content scope field applic procedur specifi refer method determin fat content raw milk milk partli skim milk skim milk definit fat content milk materi determin method express percentag mass principl ammoniac ethanol solut test portion extract diethyl ether light petroleum solvent remov distil evapor mass extract materi solubl light petroleum determin procedur roes gottlieb method reagent reagent recogn analyt qualiti leav appreci residu blank test test qualiti reagent carri determin empti flask beaker metal dish weigh prepar tare correct effect weigh result caus atmospher condit residu correct appar chang mass tare larger mg determin residu solvent separ evapor ml diethyl ether ml light petroleum tare weigh residu larger mg cleans solvent mean distil replac solvent ammonia solut approxim nh concentr ammonia solut ethanol ethanol denatur methanol provid result determin affect congo red cresol red solut dissolv congo red cresol red water dilut ml note solut interfac solvent aqueou layer option aqueou colour solut provid affect result determin diethyleth free peroxid mg kg antioxid meet requir blank test light petroleum boil rang oc mix solvent prepar shortli mix equal volum diethyl ether light petroleum apparatu glasswar warn determin involv volatil flammabl solvent electr apparatu emploi compli legisl relat solvent usual laboratori equip analyt balanc centrifug fat extract flask tube spun rotat frequenc rev min produc gravit field outer end flask tube note centrifug option distil evapor apparatu permit solvent ethanol distil flask evapor beaker dish sec temperatur exceed oc oven electr heat ventil port fulli open capabl control temperatur oc work space oven fit suitabl thermomet water bath capabl maintain temperatur oc mojonni type fat extract flask note fat extract tube siphon wash bottl fit procedur appendix flask tube provid ground glass good qualiti bark cork stopper unaffect reagent rark cork extract diethyl ether water oc minut allow cool water satur rack hold fat extract flask tube wash bottl suitabl mix solvent plastic wash bottl fat collect vessel boil flask flat bottom erlenmey flask capac ml metal dish metal dish prefer stainless steel flat bottom prefer spout diamet mm height approxim mm boil aid fat free porou porcelain silicon carbid glass bead option case metal dish measur cylind capac ml pipett graduat capac ml tong made metal suitabl hold flask beaker dish procedur note altern procedur fat extract tube siphon wash bottl fit note appendix prepar test sampl adjust temperatur laboratori sampl approxim oc minut mean water bath mix sampl gentli repeatedli invert sampl bottl caus froth churn cool quickli approxim oc test portion mix test sampl gentli invert bottl time immedi weigh nearest mg test sampl directli differ extract flask test portion deliv complet lower small bulb extract flask blank test carri blank test simultan determin procedur reagent replac test portion ml water chang appar mass fat collect vessel correct appar chang mass control vessel greater mg prepar fat collect vessel dry vessel boil aid promot gentl boil subsequ remov solvent oven hour vessel cool desiccat protect dust temperatur weigh room glass vessel hour metal dish minut take care avoid temperatur variat tong place vessel balanc weigh nearest mg determin add ml ammonia solut equival volum concentr ammonia solut mix test portion small bulb flask addit ammonia carri determin delai add ml ethanol mix gentli allow content flask flow backward forward bulb avoid bring liquid neck flask desir add drop congo red cresol red solut add ml diethyl ether close flask cork satur water stopper wet water shake flask vigor excess order avoid format persist emuls minut flask horizont posit small bulb extend upward period liquid larg bulb run small bulb cool flask run water carefulli remov cork stopper rins neck flask mix solvent wash bottl rins run flask add ml light petroleum close flask rewet cork stopper rewet dip water shake flask gentli second centrifug close flask minut rotat frequenc rev min centrifug note close flask stand rack minut supernat layer clear distinctli separ aqueou layer cool flask run water carefulli remov cork stopper rins insid neck flask mix solvent rins run flask interfac bottom neck flask rais slightli level gentli ad water side flask facilit decant solvent hold extract flask small bulb carefulli decant supernat layer prepar fat collect vessel boil aid case flask option metal dish avoid decant aqueou layer rins neck extract flask mix solvent collect rins fat collect vessel take care mix solvent spread extract flask desir solvent part solvent remov vessel distil evapor add ml ethanol content extract flask ethanol rins insid neck flask mix decrib carri extract repeat oper inclus ml diethyl ether ml light petroleum ether rins insid neck extract flask rais interfac middl stem flask enabl final decant solvent complet carri extract repeat oper inclus ml diethyl ether ml light petroleum ether rins insid neck extract flask rais interfac middl neck flask enabl final decant solvent complet extract omit skim milk remov solvent includ ethanol complet flask distil beaker dish evapor rins insid neck flask mix solvent commenc distil heat fat collect vessel flask side solvent vapour escap hour oven remov fat collect vessel oven cool desicc protect dust temperatur weigh room glass vessel hour metal dish minut weigh nearest mg wipe vessel immedi weigh place vessel balanc tong avoid temperatur variat repeat oper mass fat collect vessel decreas mg increas success weigh record minimum mass observ mass fat collect vessel extract matter add ml light petroleum fat collect vessel order verifi extract matter wholli solubl warm gentli swirl solvent fat dissolv extract matter wholli solubl light petroleum mass fat differ final mass vessel extract matter initi mass extract matter wholli solubl light petroleum case doubt extract fat complet vessel repeatedli wash warm light petroleum trace insolubl materi settl carefulli decant light petroleum remov insolubl materi repeat oper time light petroleum rins insid neck vessel final rins top vessel mix solvent solvent spread vessel remov light petroleum vapour vessel heat vessel hour oven cool weigh mass fat differ mass determin final mass express result calcul formula calcul fat content percentag mass fat content mass gram test portion mass gram fat collect vessel extract matter determin mass gram prepar fat collect vessel case undissolv materi fat collect vessel insolubl residu determin mass gram fat collect vessel blank test extract matter determin mass gram prepar fat collect vessel blank test case undissolv materi fat collect vessel insolubl residu determin report result nearest precis repeat milk partli skim milk fat product skim milk fat product reproduc milk fat product partli skim milk fat product skim milk fat product iii determin total fat solid scope field applic procedur specifi refer method determin content total fat solid heat treat milk definit calcul total fat solid content express percentag mass content fat solid content total solid section minu content fat section ii iv determin total nitrogen content scope field applic procedur specifi refer method determin total nitrogen content raw milk milk partli skim milk skim milk definit total nitrogen content milk nitrogen content express cent mass determin kjeldahl method principl weigh quantiti milk sampl digest concentr sulphur acid potassium sulphat copper ii sulphat catalyst order convert nitrogen organ compound ammonium sulphat ammonia releas addit sodium hydroxid solut distil absorb boric acid solut titrat acid solut reagent potassium sulphat copper sulphat solut dissolv copper ii sulphat pentahydr cuso water dilut ml oc volumetr flask sulphur acid sodium hydroxid solut naoh oc note concentr sodium hydroxid solut oc oc boric acid solut dissolv boric acid bo litr hot water cool store borosil glass bottl indic solut dissolv methyl red bromothymol blue bromocresol green ml ethanol store solut brown close bottl cool dark place volumetr solut hcl mol standard nearest mol nitrogen free sucros ammonium salt pure ammonium oxal nh ammonium sulfat nh tryptophan phenacetin ch conh lysin mono di hydrochlorid hcl hcl note puriti reagent higher qualiti analyt grade certifi ammonium salt solut apparatu glasswar usual laboratori equip kjeldahl flask capac ml suitabl boil aid glass bead diamet approxim mm hengar granul pumic burett automat pipett deliv ml graduat measur cylind glass capac ml digest apparatu inclin posit approxim electr heater ga burner heat flask level content fume extract system distil apparatu made borosil glass kjeldahl flask fit consist effici splash head connect effici condens straight tube outlet tube attach lower end connect tube stopper close fit prefer neopren rubber pipett automat pipett deliv ml conic flask capac ml graduat ml burett capac ml gratuat ml maximum error ml magnifi len read burett ph meter automat burett procedur kjeldahl flask add boil aid glass bead potassium sulphat ml copper sulphat solut approxim milk sampl weigh nearest ml sulphur acid acid wash copper sulphat solut potassium sulphat milk neck flask gentli mix content flask note organ matter consum sulphur acid boil ml ml digest milk fat blank test heat kjeldahl flask digest apparatu gentli black froth stai bulb initi froth ceas copiou white vapour appear boil vigor acid vapour condens half neck flask black particl remain digest clear pale blue green colour boil gentli hour note requir time digest clear hour total digest time hour hour achiev clear total digest time increas ad potassium sulphat promot digest rais boil temperatur mixtur residu volum approxim ml end digest time nitrogen lost excess heat heat ga heat flask plate heat insul materi provid circular open diamet free flame touch part flask surfac liquid content black particl enter neck flask wash bulb acid reflux init stage vigor boil period facilit rotat flask flask cool suffici carefulli wash minimum water continu digest kjeldahl flask cool add ml water note wash carefulli neck flask mix content ensur crystal separ dissolv add boil aid ensur uniform boil flask add ml sodium hydroxid solut note gentli pour solut inclin neck flask form bottom layer bulb wet top neck sodium hydroxid solut note combin volum water sodium hydroxid solut total ml enabl approxim ml distil collect irregular boil bump ensu larger equival volum sodium hydroxid solut concentr ad volum water ad reduc ml ml sodium hydroxid solut ad volum water ad ml ml immedi connect kjeldahl flask distil apparatu ensur tip condens outlet tube immers ml boric acid solut ml drop indic solut contain conic flask swirl content kjeldahl flask mix boil gentli prevent excess froth ml distil collect lower conic flask tip condens outlet tube approxim mm ml mark continu distil irregular boil bump start immedi stop heat disconnect kjeldahl flask rins tip condens outlet tube water collect rins conic flask note requir distil rate approxim ml distil collect irregular boil bump start volum content conic flask approxim ml effici condens temperatur content conic flask exce oc distil titrat distil standard volumetr solut ph ph meter desir automat burett addit indic help check titrat proceed correctli burett read nearest ml aid magnifi len avoid error parallax titrat carri indic titrat colour distil correspond solut recent prepar ml water ad ml boric acid solut ml indic contain conic flask carri blank test inclus take ml distil water sucros procedur milk sampl note titrat blank distil requir small volum standard volumetr solut regularli check accuraci procedur recoveri trial procedur inclus check loss nitrogen occur result excess heat mechan leak distil test portion ammonium oxal sulphat weigh nearest sucros percentag nitrogen recov lower higher result failur procedur inaccur concentr standard solut check digest procedur suffici releas protein nitrogen test portion pure tryptophan phenacetin lysin hydrochlorid weigh nearest nitrogen recov safeti precaut work concentr sulphur acid sodium hydroxid handl kjeldahl flask wear laboratori coat safeti goggl acid resist glove distil leav kjeldahl flask unattend potenti danger stop distil immedi flask content bump vigor power minut lower collect flask distil tip liquid express result calcul formula calcul nitrogen content wn express gram nitrogen product wn vo wn nitrogen content volum millilitr standard volumetr solut acid determin vo volum millilitr standard volumetr solut acid blank test concentr express mole litr acid standard volumetr solut mass gram test portion round result nearest precis repeat reproduc modifi procedur block digest apparatu fit cylindr flask digest apparatu kjeldahl flask case identifi potenti troubl spot check individu steam distil direct heat flask apparatu distil water care water acid alkalin volatil test portion milk semi macro kjeldahl provid amount reagent miner cuso reduc ratio total digest time reduc minut amount sodium hydroxid solut reduc ratio acid standard solut lower concentr mol note option accept repeat accuraci test result accord requir method determin protein content scope field applic procedur specifi refer method determin content protein heat treat milk articl direct eec definit protein content obtain multipli total nitrogen content express percentag mass determin accord method section iv factor calcul protein content milk percentag mass total content milk vi determin specif mass scope field applic procedur specifi refer method determin specif mass oc raw milk milk partli skim milk skim milk definit specif mass milk ratio mass volum milk oc mass volum water oc principl specif mass oc determin hydromet apparatu glasswar usual laboratori equip hydromet specif graviti hydromet instrument consist glass float lower end wide heavi cylindr shape glass rod attach upper float coaxial orient upper end rod close glass float load lead mercuri intend adjust hydromet mass rod includ graduat scale ml hydromet check pycnometr method pycnomet capac approxim ml equip precis thermomet cylind glass stainless steel minimum dimens intern diamet approxim mm intern height approxim mm water bath regul oc water bath regul oc thermomet graduat oc procedur mix sampl invers dispers fat place water bath sampl reach temperatur oc maintain temperatur minut mix care invers ensur fat homogen distribut cool oc water bath mix sampl care invers avoid inclus air pour milk cylind held inclin avoid format foam bubbl suffici milk sampl ensur overflow cylind hydromet carefulli lower hydromet milk float freeli reach balanc posit cylind vertic hydromet posit middl liquid column touch side cylind hydromet reach equilibrium read graduat top meniscu immedi take hydromet read introduc thermomet sampl read temperatur accuraci oc temperatur differ oc oc temperatur correct temperatur milk sampl oc measur specif mass made result obtain correct ad determin specif mass degre celciu oc subtract degre celsiu oc correct valid temperatur milk sampl differ oc oc express result method calcul formula specif mass sampl express ml skim milk oc formula mv mg mv mv mg mg mv mg mv mv specif mass sampl read hydromet mg fat content sampl densiti fat precis repeat ml reproduc ml appendix annex ii altern procedur fat extract tube siphon wash bottl fit procedur prepar test sampl test portion proce fat extract tube test portion deliv complet bottom extract tube blank test prepar fat collect vessel determin add ml ammonia solut equival volum concentr ammonia solut mix pretreat test portion bottom tube addit ammonia carri determin delai add ml ethanol mix gentli bottom tube desir add drop congo red cresol red solut add ml diethyl ether close tube cork satur water stopper wet water shake tube vigor excess order avoid format persist emuls repeat invers minut cool tube run water carefulli remov cork stopper rins neck tube mix solvent wash bottl rins run tube add ml light petroleum close tube rewet cork stopper rewet dip water shake tube gentli second centrifug close tube minut rotat frequenc rev min centrifug note close tube stand rack minut supernat layer clear distincli separ aqueou layer cool tube run water carefulli remov cork stopper rins neck tube mix solvent rins run tube insert siphon fit wash bottl fit tube push long limb fit inlet approxim mm interfac layer limb fit parallel axi extract tube carefulli transfer supernat layer tube prepar fat collect vessel boil aid case flask option metal dish avoid transfer aqueou layer rins outlet fit mix solvent collect rins fat collect vessel loosen fit neck tube slightli rais fit rins lower part long limb mix solvent lower insert fit transfer rins fat collect vessel rins outlet fit mix solvent collect rins vessel desir solvent part solvent remov vessel distil evapor loosen fit neck slightli rais fit add ml ethanol content tube ethanol rins long limb fit mix carri extract repeat oper ml diethyl ether ml light petroleum ether rins long limb fit remov fit tube previou extract carri extract repeat oper ml diethyl ether ml light petroleum rins long limb fit extract omit skim milk proce