regul ec european parliament council march deterg text eea relev european parliament council european union regard treati establish european commun articl thereof regard propos commiss regard opinion european econom social committe act accord procedur laid articl treati council direct eec novemb approxim law member state relat deterg eec novemb approxim law member state relat method test biodegrad anion surfact eec march approxim law member state relat method test biodegrad ionic surfact eec march amend direct eec approxim law member state relat method test biodegrad anion surfact eec march amend time direct eec approxim law member state relat deterg significantli amend occas desir reason clariti rationalis provis question recast bring singl text commiss recommend eec septemb label provis deterg clean product includ singl text object regul ensur intern market deterg suffici achiev member state common technic criteria commun achiev commun level commun adopt measur accord principl subsidiar set articl treati accord principl propotion set articl regul order achiev object regul legal instrument impos directli manufactur precis requir implement time manner commun area technic legisl uniform applic member state need guarante regul definit deterg need cover equival line develop member state level introduc surfact definit lack exist legisl import give clear precis descript relev type biodegrad measur deterg adopt ensur function intern market avoid restrict competit commun confirm commiss white paper strategi futur chemic polici measur deterg ensur high level environment protect aquat environ deterg subject commun provis manufactur proper handl usag label refer commiss recommend eec commiss recommend ec juli good environment practic household laundri deterg direct ec european parliament council approxim law regul administr provis member state relat classif packag label danger prepar appli deterg ditallow dimethyl ammonium chlorid dtdmac nonylphenol includ ethoxyl deriv ap prioriti substanc undergo commun level risk assess activ accord council regul eec march evalu control risk exist substanc adequ strategi limit risk exposur substanc recommend implement framework commun provis exist legisl biodegrad surfact deterg cover primari biodegrad applic anion ionic surfact replac legisl lai main emphasi ultim biodegrad meet import concern relat potenti toxic persist metabolit requir introduct set test base en iso standard oecd guidelin govern grant direct permiss place deterg market provid high level protect environ deterg fulfil requir laid regul market novemb scientif committe toxic ecotox environ issu opinion biodegrad surfact deterg relev test method regulatori control area exist requir primari biodegrad maintain hierarchi level supplement complementari risk assess surfact fail ultim biodegrad test surfact fail primari biodegrad test obtain market authoris derog primari biodegrad requir extend surfact cation amphoter whilst allow possibl appli instrument analys case semi specif analyt method suitabl determin biodegrad test method record keep list derog technic matter revis take account technic scientif develop regulatori develop test method produc data give suffici assur aerob biodegrad surfact deterg method test biodegrad surfact deterg produc variabl result case complement addit assess order determin risk continu provis laid place market except case surfact deterg fail ultim biodegrad test place basi relev inform ensur environment protect case case basi measur implement regul adopt accord council decis ec june lai procedur exercis implement power confer commiss recal horizont legisl applic deterg surfact council direct eec juli approxim law regul administr provis member state relat restrict market danger substanc prepar market danger substanc cover regul ban restrict council direct eec june approxim law regul administr provis relat classif packag label danger substanc commiss direct eec juli lai principl assess risk man environ substanc notifi accord council direct eec regul eec commiss regul ec june lai principl assess risk man environ exist substanc direct ec european parliament council februari place biocid product market direct ec european parliament council februari harmonis law regul administr provis relat applic principl good laboratori practic verif applic test chemic substanc codifi version direct ec european parliament council februari inspect verif good laboratori practic glp codifi version council direct eec novemb approxim law regul administr provis member state protect anim experiment scientif purpos respons manufactur refrain market deterg compli regul dispos nation author technic file substanc prepar cover regul appli surfact fail pass test mention annex iii manufactur request derog commiss possibl grant derog accord procedur refer articl member state compet author appli control measur deterg market avoid repeat test made compet laboratori exist label provis deterg continu includ recommend eec includ regul order fulfil object modernis rule deterg specif label introduc inform consum fragranc substanc preserv agent present deterg medic personnel obtain manufactur request full list ingredi deterg assist investig causal link exist develop allerg respons exposur chemic substanc member state requir list made specif public bodi design provid inform medic personnel point call legisl replac exist legisl period member state continu appli exist law technic annex regul adapt accord procedur refer articl deterg compli regul allow market prejudic relev commun provis order ensur protect man environ unforeseen risk deterg safeguard claus need test biodegrad surfact carri laboratori meet internation recognis standard en iso iec principl good laboratori practic justifi applic requir exist surfact extent test perform enter forc standard provid compar level scientif qualiti issu relat anaerob biodegrad biodegrad main surfact organ deterg ingredi phosphat content dealt regul examin commiss justifi propos present european parliament council pend harmonis member state maintain lai nation rule issu direct commiss recommend mention recit replac regul repeal adopt regul articl object scope regul establish rule design achiev free movement deterg surfact deterg intern market time ensur high degre protect environ human health purpos regul harmonis rule place market deterg surfact deterg biodegrad surfact deterg restrict ban surfact ground biodegrad addit label deterg includ fragranc allergen inform manufactur hold dispos member state compet author medic personnel articl definit purpos regul quot deterg quot mean substanc prepar soap surfact intend wash clean process deterg form liquid powder past bar cake mould piec shape market household institut industri purpos product consid deterg quot auxiliari wash prepar quot intend soak pre wash rins bleach cloth household linen quot laundri fabric soften quot intend modifi feel fabric process complement wash fabric quot clean prepar quot intend domest purpos cleaner clean surfac materi product machineri mechan applianc mean transport equip instrument apparatu quot clean wash prepar quot intend wash clean process quot wash quot mean clean laundri fabric dish hard surfac quot clean quot mean defin en iso quot substanc quot mean chemic element compound natur state obtain product process includ addit preserv stabil product impur deriv process exclud solvent separ affect stabil substanc chang composit quot prepar quot mean mixtur solut compos substanc quot surfact quot mean organ substanc prepar deterg surfac activ properti consist hydrophil hydrophob group natur size capabl reduc surfac tension water form spread adsorpt monolay water air interfac form emuls microemuls micel adsorpt water solid interfac quot primari biodegrad quot mean structur chang transform surfact micro organ result loss surfac activ properti due degrad parent substanc consequenti loss surfac activ properti measur test method list annex ii quot ultim aerob biodegrad quot mean level biodegrad achiev surfact total micro organ presenc oxygen result breakdown carbon dioxid water miner salt element present mineralis measur test method list annex iii microbi cellular constitu biomass quot place market quot mean introduc commun market make parti exchang payment import commun custom territori deem place market quot manufactur quot mean natur legal person respons place deterg surfact deterg market produc import packag work account person chang characterist deterg surfact deterg creat chang label thereof deem manufactur distributor chang characterist label packag deterg surfact deterg deem manufactur act import quot medic personnel quot mean regist medic practition person work direct regist medic practition act provid patient care make diagnosi administ treatment bound profession confidenti quot industri institut deterg quot mean deterg wash clean domest sphere carri specialis personnel specif product articl place market market deterg surfact deterg refer articl conform condit characterist limit laid regul annex relev direct ec relev commun legisl surfact activ substanc mean direct ec disinfect exempt provis annex ii iii iv viii regul provid list annex ia direct ec constitu biocid product authoris articl direct ec constitu biocid product allow transit measur subject year work programm provid articl direct ec surfact deem disinfect deterg ingredi subject label provis disinfect annex vii manufactur deterg surfact deterg establish commun manufactur respons conform deterg surfact deterg provis regul annex articl limit base biodegrad surfact regul surfact deterg surfact meet criteria ultim aerob biodegrad laid annex iii market limit relat biodegrad deterg surfact level ultim aerob biodegrad lower stipul annex iii manufactur industri institut deterg surfact surfact industri institut deterg derog request derog made decid accord articl level primari biodegrad measur surfact deterg fail ultim aerob biodegrad test deterg surfact level primari biodegrad lower stipul annex ii grant derog articl grant derog request manufactur derog made send applic compet author member state concern refer articl commiss provid evid relat criteria mention articl member state make request derog depend payment member state compet author fee fee levi discriminatori exce cost process applic applic includ technic file suppli inform justif evalu safeti aspect relat specif surfact deterg fail compli biodegrad limit set annex iii addit result test stipul annex iii technic file includ inform result test stipul annex ii iv test laid annex iv point carri basi tier approach tier approach defin technic guidanc document adopt accord procedur refer articl april guidanc document test principl good laboratori practic appli compet author member state receiv applic derog accord paragraph examin request evalu complianc condit derog inform commiss result month receiv complet applic compet author member state deem evalu risk caus substanc prepar month receiv applic inform verif confirmatori test substanc prepar transform product notifi receiv inform regul time period evalu dossier compet author member state start dossier complet addit inform request inform provid month applic consid incomplet invalid case articl appli inform metabolit sought stepwis test strategi emploi ensur maximum vitro anim test method basi evalu carri member state commiss grant derog accord procedur refer articl grant derog commiss evalu matter paragraph articl decis month receiv evalu member state case articl decis ec period month derog limit sever restrict place market surfact ingredi deterg depend result complementari risk assess defin annex iv includ phase period place market surfact ingredi deterg commiss review derog inform light justifi signific revis technic file includ applic derog purpos manufactur request suppli commiss technic file updat item mention annex iv point basi updat inform commiss decid prolong modifi termin derog paragraph articl articl appli mutati mutandi commiss publish list surfact obtain derog condit limit provid annex articl condit grant derog commiss consid grant derog accord procedur refer articl basi criteria low dispers applic wide dispers applic specif industri institut applic risk environ health pose volum sale pattern commun small compar socio econom benefit includ food safeti hygien standard long commiss decid request derog place market surfact question maintain provid manufactur show surfact commun market date entri forc regul request derog made year date commiss refus grant derog month receiv member state evalu mention articl case articl decis ec period month set transit period place market surfact question phase transit period exce year date commiss decis commiss publish annex vi list surfact identifi compli regul articl test surfact test refer articl annex ii iii iv viii conduct complianc standard mention annex accord test requir articl regul eec purpos suffici appli en iso iec standard principl good laboratori practic test principl good laboratori practic made mandatori case surfact deterg market entri forc standard exist test perform scientif knowledg perform standard compar standard mention annex accept case case basi manufactur member state submit commiss case doubt disput decis accord procedur laid articl articl duti member state member state appoint compet author author respons commun exchang inform relat manag regul inform commiss full address author member state notifi member state commiss list approv laboratori full address compet authoris carri test requir regul member state demonstr compet laboratori standard en iso iec mention annex requir deem fulfil member state verifi complianc laboratori principl good laboratori practic accord articl direct ec compet author member state ground believ approv laboratori possess compet refer paragraph rais matter committe refer articl commiss decid laboratori possess requir compet approv laboratori remov list refer paragraph articl appli case laboratori claim complianc requir good laboratori practic complianc provis articl direct ec appli commiss publish list compet author mention paragraph approv laboratori mention paragraph year offici journal european union extent occur articl inform provid manufactur prejudic articl direct ec manufactur place market substanc prepar cover regul hold dispos compet author member state inform result test mention annex iii surfact fail pass test mention annex iii request derog made refer articl technic file result test mention annex ii ii technic file result test inform mention annex iv substanc prepar cover regul market manufactur respons correct perform relev test mention document test carri demonstr complianc regul show allow benefit properti right test result test result public domain manufactur place market prepar cover regul request make delai free charg medic personnel ingredi datasheet stipul annex vii prejudic member state request datasheet made specif public bodi member state assign task provid inform medic personnel inform contain datasheet confidenti specif public bodi medic personnel medic purpos articl control measur member state compet author appli control measur deterg market ensur complianc product provis regul refer method test analyt method refer annex viii control measur oblig manufactur repeat test made laboratori fulfil condit articl pai repeat addit test provid initi test shown complianc deterg surfact ingredi deterg regul case concern test carri accord method list annex ii iii iv viii produc fals posit result member state compet author notifi commiss commiss accord procedur laid articl verifi result measur articl label provis articl prejudic provis relat classif packag label danger substanc prepar direct eec ec inform legibl visibl indel charact packag deterg put sale consum trade product trade trademark full address telephon number parti respons place product market address email address telephon number datasheet refer articl obtain inform document accompani deterg transport bulk packag deterg content accord specif provid annex vii instruct special precaut requir addition packag deterg sold gener public intend laundri deterg bear inform provid annex vii case member state nation requir label nation languag manufactur distributor compli requir inform paragraph paragraph prejudic exist nation rule graphic represent fruit lead user error liquid product packag deterg put sale consum articl committe procedur commiss assist committe refer made paragraph articl decis ec appli regard provis articl thereof period laid articl decis ec set month committe adopt rule procedur articl adapt annex amend adapt annex adopt accord procedur refer articl european standard amend addit appli rule regul solvent base deterg adopt accord procedur refer articl articl free movement claus member state prohibit restrict imped place market deterg surfact deterg compli requir regul ground dealt regul pend harmonis member state maintain lai nation rule phosphat deterg articl safeguard claus member state justifi ground believ specif deterg compli requir regul constitut risk safeti health human anim risk environ temporarili prohibit place market deterg territori make temporarili subject special condit immedi inform member state commiss thereof give reason decis consult member state relev technic scientif committe commiss decis matter nineti dai accord procedur refer articl articl review april commiss evalu submit report justifi present legisl propos phosphat view gradual phase restrict specif applic april commiss carri review applic regul pai regard biodegrad surfact evalu submit report justifi present legisl propos relat anaerob biodegrad biodegrad main surfact organ deterg ingredi articl legisl repeal direct repeal effect octob direct eec direct eec direct eec direct eec direct eec recommend eec repeal effect octob refer made repeal direct constru refer regul dai entri forc regul member state repeal law regul administr provis adopt pursuant direct provid paragraph recommend refer paragraph articl sanction octob legal administr measur order deal infring regul dissuas effect proportion sanction infring includ measur allow detain consign deterg fail compli regul immedi inform commiss thereof articl entri forc regul enter forc octob regul bind entireti directli applic member state strasbourg march european parliament presid cox council presid roch oj opinion european parliament april publish offici journal council common posit novemb oj posit european parliament januari publish offici journal decis council march oj direct amend regul ec oj oj direct amend direct eec oj oj oj oj oj oj oj direct amend regul ec european parliament council oj oj regul amend regul ec direct eec eec direct eec eec direct eec oj oj direct amend commiss direct ec oj oj direct amend regul ec oj oj oj direct amend regul ec oj oj oj direct amend direct ec european parliament council oj annex standard accredit good laboratori practic anim protect laboratori compet authoris provid servic check complianc deterg requir regul annex standard applic level laboratori en iso iec gener requir compet test calibr laboratori direct ec council direct eec standard applic level accredit bodi good laboratori practic monitor author en calibr test laboratori accredit system gener requir oper recognit direct ec annex ii primari biodegrad test method surfact deterg primari biodegrad measur determin biodegrad liquor remain level parent surfact annex begin list test method common class surfact list head analyt test procedur specif class surfact pass criterion primari biodegrad level measur test method refer method laboratori test surfact regul base confirmatori test procedur oecd method annex viii confirmatori test procedur permiss provid compli en iso test method oecd method publish oecd technic report june quot propos method determin biodegrad surfact synthet deterg quot method franc approv quot arrêté du décembr quot publish journal officiel de la républiqu français decemb standard nf june publish associ français de normalis afnor method germani establish quot verordnung über die abbaubarkeit anionisch und nichtionisch grenzflächenaktiv stoff wasch und reinigungsmitteln quot januari publish bundesgesetzblatt part set regul amend regul june publish bundesgesetzblatt part method unit kingdom call quot porou pot test quot technic report water research centr quot confirmatori test procedur quot oecd method annex viii includ oper condit propos en iso refer method settlement litig analyt method anion surfact determin anion surfact test methylen blue activ substanc mba analysi criteria establish annex viii anion surfact react abovement mba method reason effici precis specif instrument analys high perform liquid chromatographi hplc ga chromatographi gc appli sampl pure surfact interest provid manufactur compet author member state request analyt method ionic surfact determin ionic surfact test bismuth activ substanc bia method analyt procedur establish annex viii ionic surfact react abovement bia method reason effici precis specif instrument analys hplc gc appli sampl pure surfact interest provid manufactur compet author member state request analyt method cation surfact determin cation surfact test disulfin blue activ substanc dba analysi dba procedur method feder republ germani din ausgab cation surfact react abovement test method reason effici precis justifi specif instrument analys hplc gc appli sampl pure surfact interest provid manufactur compet nation author member state request analyt method amphoter surfact determin amphoter surfact test analysi procedur list cation absent method feder republ germani din teil orang ii method boiteux amphoter surfact react abovement test reason effici precis justifi specif instrument analys hplc gc appli sampl pure surfact interest provid manufactur compet author member state request annex iii ultim biodegrad mineralis test method surfact deterg refer method laboratori test surfact ultim biodegrad regul base en iso standard headspac test surfact deterg consid biodegrad level biodegrad mineralis measur test twenti dai en iso standard water qualiti evalu ultim aerob biodegrad organ compound aqueou medium method analysi inorgan carbon seal vessel headspac test pre adapt ten dai window principl appli refer method method direct eec annex carbon dioxid evolut modifi sturm test pre adapt ten dai window principl appli method direct eec annex close bottl pre adapt ten dai window principl appli method direct eec annex manometr respirometri pre adapt ten dai window principl appli method direct eec annex miti ministri intern trade industri japan pre adapt ten dai window principl appli depend physic characterist surfact method list appropri justifi note pass criterion method consid equival pass criterion refer method list point adequaci choic method list decid case case confirm accord articl regul method direct eec annex dissolv organ carbon doc die pre adapt ten dai window principl appli pass criteria biodegrad measur test twenti dai method direct eec annex modifi oecd screen doc die pre adapt ten dai window principl appli pass criteria biodegrad measur test twenti dai abovement method council direct eec found public quot classif packag label danger substanc european union quot part quot test method quot european commiss isbn test identifi suitabl surfact doc method give result remov ultim biodegrad manometr respirometri miti case high initi test concentr inhibitori annex iv complementari risk assess surfact deterg surfact environment risk assess context direct eec regul eec regul ec technic guidanc document risk assess consid complementari risk assess run scope regul complementari risk assess run scope regul case recalcitr metabolit produc consid context assess made basi direct eec regul eec assess case case basi result test refer part studi cover aquat environment compart addit inform relat specif risk assess concern requir committe refer articl case case basi addit inform includ environment compart sewag sludg soil tier approach adopt inform requir technic file refer articl file inform point minimis test avoid unnecessari anim test addit studi list point request inform proportion case disput extent addit inform requir decis accord procedur laid articl note articl guidelin includ annex decis derog adapt basi accumul experi ident surfact accord provis laid annex vii direct eec name iupac nomenclatur name ca number ca einec elinc number molecular structur formula composit surfact inform surfact quantiti surfact deterg inform pattern section suffici approxim realist estim function environment exposur surfact deterg includ import applic societ condit releas scenario volum avail suitabl altern perform econom consider assess relev environment inform inform potenti recalcitr metabolit toxic inform test liquor provid data residu ident inform refer point request depend potenti risk import quantiti surfact deterg conflict case inform decis accord procedur laid articl addit studi biodegrad test pre adapt inoculum test annex iii run pre adapt inoculum order provid evid relev pre adapt surfact inher biodegrad test test refer includ method direct eec annex modifi sca test method direct eec annex zahn wellen failur pass inher biodegrad test potenti persist consid gener term suffici prohibit place market surfact case criteria set articl justif refus derog activ sludg simul biodegrad test test refer includ method direct eec annex includ oper condit propos en iso failur pass activ sludg simul biodegrad test potenti releas metabolit sewag treatment consid gener term evid complet risk assess toxic test biodegrad test liquor toxic inform test liquor provid chemic physic inform ident metabolit analyt mean obtain kei physic chemic properti water solubl octanol water partit coeffici log po effect organ test conduct complianc principl good laboratori practic fish test recommend annex direct eec daphnia test recommend annex direct eec alga test recommend annex direct eec bacteria test recommend annex direct eec degrad biotic test recommend annex direct eec abiot test recommend annex direct eec inform provid potenti metabolit bio concentr partit sediment phase metabolit suspect endocrin disrupt activ recommend determin potenti result advers affect valid test scheme assess advers effect abovement test consult public classif packag label danger substanc european union part quot test method quot european commiss isbn european inventori exist commerci substanc european list notifi chemic substanc annex list surfact obtain derog deterg surfact pass test stipul annex ii pass test stipul annex iii market mean derog stipul articl accord procedur laid articl gt pic file quot en tif quot gt quot einec quot mean european inventori exist commerci substanc inventori definit list substanc deem commun market septemb quot elinc quot mean list substanc defin council direct eec april amend seventh time direct eec approxim law regul administr provis relat classif packag label danger substanc oj annex vi list ban restrict deterg surfact deterg surfact identifi compli provis regul gt pic file quot en tif quot gt quot einec quot mean european inventori exist commerci substanc inventori definit list substanc deem commun market septemb quot elinc quot mean list substanc defin direct eec annex vii label ingredi datasheet label content provis label appli packag deterg sold gener public weight percentag rang content constitu list ad concentr weight phosphat phosphon anion surfact cation surfact amphoter surfact ionic surfact oxygen base bleach agent chlorin base bleach agent edta salt thereof nta nitrilotriacet acid salt thereof phenol halogen phenol paradichlorobenzen aromat hydrocarbon aliphat hydrocarbon halogen hydrocarbon soap zeolit polycarboxyl class constitu ad list irrespect concentr enzym disinfect optic brighten perfum ad preserv agent list irrespect concentr common nomenclatur establish articl council direct eec juli approxim law member state relat cosmet product ad concentr exceed weight allergen fragranc list substanc annex iii part direct eec result amend direct ec european parliament council includ allergen perfum ingredi list establish scientif committe cosmet food product sccnfp opinion sccnfp list nomenclatur direct fragranc subsequ ad annex iii part direct eec adapt annex technic progress individu risk base concentr limit fragranc allergen subsequ establish sccnfp commiss propos adopt accord articl limit replac limit mention deterg intend industri sector made member gener public abovement requir fulfil equival inform provid mean technic data sheet safeti data sheet similar manner label dosag inform prescrib articl provis label appli packag deterg sold gener public packag deterg sold gener public intend laundri deterg bear inform recommend quantiti dosag instruct express millilitr gram standard wash machin load soft medium hard water hard class make provis cycl wash process heavi duti deterg number standard wash machin load quot soil quot fabric deterg delic fabric number standard wash machin load lightli soil fabric wash content packag water medium hard millimol caco capac measur cup provid millilitr gram mark provid dose deterg standard wash machin load soft medium hard water hard class standard wash machin load kg dry fabric heavi duti deterg kg dry fabric low duti deterg line definit commiss decis ec june establish ecolog criteria award commun eco label laundri deterg deterg consid heavi duti deterg claim manufactur predominantli promot fabric care low temperatur wash delic fibr colour ingredi datasheet provis appli list ingredi datasheet refer articl datasheet list deterg manufactur ingredi list order decreas abund weight list divid weight percentag rang impur consid ingredi common chemic iupac ca number inci european pharmacopoeia ingredi public list ingredi manufactur make websit ingredi datasheet mention inform weight percentag rang constitu perfum essenti oil constitu colour agent oblig appli industri institut deterg surfact surfact industri institut deterg technic data sheet safeti data sheet oj direct amend commiss direct ec oj oj oj decis amend decis ec oj intern union pure appli chemistri intern nomenclatur cosmet ingredi annex viii test method analyt method test analyt method appli control procedur deterg market carri member state refer method confirmatori test definit method describ laboratori model activ sludg secondari settler design simul municip sewag treatment condit direct preced regul improv state art oper condit appli test method en iso equip need measur method measur emploi small activ sludg plant shown figur greater detail figur equip consist sewag vessel synthet sewag dose pump aerat vessel settl vessel air lift pump recycl activ sludg vessel collect treat effluent vessel glass suitabl plastic hold twenti litr pump provid constant flow synthet sewag aerat vessel vessel normal oper litr mix liquor sinter aerat cube suspend vessel apex cone quantiti air blown aerat monitor mean flow meter synthet sewag synthet sewag emploi test dissolv litr tap water mg pepton mg meat extract mg urea nh mg sodium chlorid nacl mg calcium chlorid cacl mg magnesium sulphat mgso mg di potassium hydrogen phosphat hpo mg surfact synthet sewag freshli prepar daili prepar sampl uncompound surfact examin origin state activ content surfact sampl determin order prepar synthet sewag oper equip initi fill aerat vessel settl vessel synthet sewag height vessel fix volum contain aerat vessel litr inocul made introduc ml secondari effluent good qualiti freshli collect treatment plant deal predominantli domest sewag effluent aerob condit period sampl applic set aerat air lift dose devic oper synthet sewag pass aerat vessel rate litr hour retent time hour rate aerat regul content vessel constantli suspens dissolv oxygen content mg foam prevent mean anti foam agent inhibit activ sludg surfact air lift pump set activ sludg settl vessel continu regularli recycl aerat vessel sludg accumul top aerat vessel base settl vessel circul circuit return circul dai brush mean sludg fail settl settleabl increas addit ml portion solut ferric chlorid repeat effluent settl vessel accumul vessel twenti hour sampl mix vessel carefulli clean check measur equip surfact content mg synthet sewag determin immedi surfact content mg effluent collect twenti hour vessel determin analyt method immedi collect sampl preserv prefer freez concentr determin nearest mg surfact check effici process chemic oxygen demand cod dissolv organ carbon doc glass fibr filter effluent accumul vessel filter synthet sewag vessel measur week reduct cod doc level roughli regular daili surfact degrad obtain end run period shown figur content dry matter activ sludg contain aerat vessel determin week excess activ sludg discard degrad test perform room temperatur steadi calcul biodegrad percentag degrad surfact calcul dai basi surfact content mg synthet sewag effluent accumul vessel degrad valu obtain present graphic figur degrad surfact calcul arithmet valu obtain twenti dai follow run acclimatis period degrad regular oper plant troubl free event durat run period exce week daili degrad valu calcul nearest final result nearest number case permiss reduc frequenc sampl fourteen result collect twenti dai follow run period calcul averag determin anion surfact biodegrad test principl method base fact cation dye methylen blue form blue salt anion surfact mba extract chloroform elimin interfer extract effect alkalin solut extract shaken acid methylen blue solut absorb separ organ phase measur photometr wavelength maximum absorpt nm reagent equip buffer solut ph dissolv sodium bicarbon nahco ar anhydr sodium carbon na ar deionis water dilut ml neutral methylen blue solut dissolv methylen blue ar deionis water dilut ml prepar solut twenti hour absorb blank chloroform phase measur chloroform exce cm layer thick nm acid methylen blue solut dissolv methylen blue ar ml deionis water mix ml ml dilut ml deionis water prepar solut twenti hour absorb blank chloroform phase measur chloroform exce cm layer thick nm chloroform trichloromethan ar freshli distil dodecyl benzen sulphon acid methyl ester ethanol potassium hydroxid solut koh ethanol pure sulphur acid phenolphthalein solut dissolv phenolphthalein ml ethanol add ml deionis water stir continu filter precipit obtain methanol hydrochlor acid ml hydrochlor acid ar ml methanol separ funnel ml graduat flask ml graduat flask ml graduat flask ml round bottom flask ground glass stopper reflux condens ml boil granul ph meter photomet measur nm cm cell qualit grade filter paper procedur sampl analysi layer foam clean water equip analysi rins methanol hydrochlor acid deionis water filter activ sludg plant influent effluent examin immedi sampl discard ml filtrat place measur volum sampl neutralis ml separ funnel volum sampl mba lower mba content ml sampl ml dilut ml deionis water add sampl ml buffer solut ml neutral methylen blue solut ml chloroform shake mixtur uniformli vigor minut phase separ run chloroform layer separ funnel ml deionis water ml acid methylen blue solut shake mixtur minut pass chloroform layer cotton wool filter previous clean wet chloroform graduat flask extract alkalin acid solut time ml chloroform extract filter combin chloroform extract cotton wool filter dilut mark ml flask chloroform rewash cotton wool measur absorb chloroform solut photomet nm cm cell chloroform run blank determin procedur calibr curv prepar calibr solut standard substanc dodecylbenzen sulphon acid methyl ester tetrapropylen type mol wt saponif potassium salt mba calcul sodium dodecyl benzen sulphon mol wt weigh pipett weigh mg dodecyl benzen sulphon acid methyl ester nearest mg round bottom flask add ml ethanol potassium hydroxid solut boil granul mount reflux condens boil hour cool wash condens ground glass joint ml ethanol add wash content flask titrat solut sulphur acid phenolphthalein colourless transfer solut ml graduat flask dilut mark deionis water mix part surfact stock solut dilut withdraw ml transfer ml graduat flask dilut mark deionis water mix standard solut gt refer graphic gt sampl weight mg establish calibr curv withdraw ml portion standard solut dilut ml deionis water proce state item includ blank determin calcul result amount anion surfact mba sampl read calibr curv mba content sampl gt refer graphic gt ml volum sampl express result sodium dodecylbenzen sulphon mw express result express result mba mg nearest determin ionic surfact biodegrad test liquor principl surfac activ agent concentr isol ga strip sampl quantiti ionic surfact rang strip surfact dissolv ethyl acet phase separ evapor solvent ionic surfact precipit aqueou solut modifi dragendorff reagent kbii bacl glacial acet acid precipit filter wash glacial acet acid dissolv ammonium tartrat solut bismuth solut titrat potentiometr pyrrolidinedithiocarbam solut ph bright platinum indic electrod calomel silver silver chlorid refer electrod method applic ionic surfact alkylen oxid group titrat result multipli empir factor convers refer substanc nonylphenol condens mol ethylen oxid np reagent equip reagent made deionis water pure ethyl acet freshli distil sodium bicarbon nahco ar dilut hydrochlor acid ml concentr acid hcl dilut ml water methanol ar freshli distil store glass bottl bromocresol purpl ml methanol precipit agent precipit agent mixtur volum solut volum solut mixtur store brown bottl week mix solut dissolv bismuth nitrat biono ar ml glacial acet acid make ml water dissolv potassium iodid ar ml water mix solut ml measur flask add ml glacial acet acid make ml water solut dissolv barium chlorid bacl ar ml water glacial acet acid lower concentr unsuit ammonium tartrat solut mix tartar acid ar ml ammonia solut ar ml make ml water equival amount ammonium tartrat ar dilut ammonia solut ml ammonia solut ar ml dilut ml water standard acet buffer dissolv solid sodium hydroxid ar ml water beaker cool add ml glacial acet acid mix cool transfer ml volumetr flask make mark water pyrrolidinedithiocarbam solut quot carbat solut quot dissolv mg sodium pyrrolidinedithiocarbam nna ml water add ml amyl alcohol ar nahco ar make ml water copper sulphat solut standardis stock solut mix copper sulphat cuso ar ml sulphur acid make ml water standardsolut mix ml stock solut ml make ml water sodium chlorid ar ga strip apparatu figur diamet sinter disc intern diamet cylind separ funnel ml magnet stirrer magnet mm gooch crucibl diamet perfor base mm type circular glass fibr filter paper mm diamet fibr diamet filter flask adapt rubber collar ml record potentiomet fit bright platinum indic electrod calomel silver silver chlorid refer electrod mv rang automat burett ml capac altern manual equip method concentr separ surfact filter aqueou sampl qualit filter paper discard ml filtrat strip apparatu previous rins ethyl acet place measur quantiti sampl ionic surfact improv separ add sodium chlorid sodium bicarbon volum sampl exce ml add salt strip apparatu solid form dissolv pass nitrogen air smaller size sampl dissolv salt ml water add strip apparatu add water bring level upper stopcock cautious add ml ethyl acet top water fill wash bottl ga line nitrogen air third full ethyl acet pass ga stream apparatu flowmet recommend rate aerat increas gradual begin ga rate adjust phase remain notic separ minimis mix phase solut ethyl acet water stop ga flow minut reduct volum organ phase solut water sublat repeat pai special attent rate ga flow run organ phase separ funnel return water separ funnel aqueou phase ml strip apparatu filter ethyl acet phase dry qualit filter paper ml beaker put ml ethyl acet strip apparatu pass nitrogen air minut draw organ phase separ funnel separ reject aqueou phase run organ phase filter ethyl acet portion rins separ funnel filter ml ethyl acet evapor ethyl acet extract dryness water bath fume cupboard direct gentl stream air surfac solut acceler evapor precipit filtrat dissolv dry residu ml methanol add ml water ml dilut hcl stir mixtur magnet stirrer solut add ml precipit agent measur cylind precipit form repeat stir stir ten minut leav mixtur stand minut filter mixtur gooch crucibl base cover glass fibr filter paper wash filter suction ml glacial acet acid wash beaker magnet crucibl glacial acet acid ml quantit transfer precipit adher side beaker filter solut precipit titrat return precipit beaker remain precipit dissolv dissolut precipit dissolv precipit filter crucibl addit hot ammonium tartrat solut portion ml portion stand crucibl minut suck filter flask put content filter flask beaker precipit rins side beaker ml tartrat solut dissolv rest precipit carefulli wash crucibl adapt filter flask ml water return rins water beaker precipit titrat stir solut magnet stirrer add drop bromocresol purpl add dilut ammonia solut colour turn violet solut initi weakli acid residu acet acid rins add ml standard acet buffer immers electrod solut titrat potentiometr standard quot carbat solut quot burett tip immers solut titrat rate exce ml min endpoint intersect tangent branch potenti curv observ occasion inflect potenti curv flatten elimin carefulli clean platinum electrod polish emeri paper blank determin time run blank determin procedur ml methanol ml water instruct blank titrat ml puriti reagent suspect content heavi metal replac blank account calcul result control factor quot carbat solut quot determin factor carbat solut dai titrat ml copper sulphat solut quot carbat solut quot addit ml water ml standard acet buffer amount ml factor gt refer graphic gt result titrat multipli factor calcul result ionic surfact factor depend composit length alken oxid chain concentr ionic surfact express relat standard substanc nonyl phenol ten ethylen oxid unit np convers factor factor amount surfact present sampl found express mg np equival xfx mg ionic surfact np volum quot carbat solut quot sampl ml volum quot carbat solut quot blank ml factor quot carbat solut quot express result express result mg np nearest preliminari treatment anion surfact test preliminari note treatment sampl treatment anion surfac activ agent formul deterg prior determin primari biodegrad confirmatori test gt tabl gt purpos alcohol extract elimin insolubl inorgan ingredi commerci product circumst upset biodegrad test ion exchang procedur isol separ anion surfac activ agent soap ionic cation surfact requir correct biodegrad test achiev ion exchang techniqu macro porou exchang resin suitabl eluant fraction elut soap anion ionic surfact isol procedur analyt control homogenis concentr anion surfact synthet deterg determin mba analyt procedur soap content determin suitabl analyt method analysi product calcul quantiti requir prepar fraction biodegrad test quantit extract anion surfact extract obtain principl homogen sampl powder dri past dri liquid ethanol extract obtain surfact soap alcohol solubl constitu synthet deterg sampl ethanol extract evapor dryness dissolv isopropanol water mixtur solut obtain pass strongli acid cation exchang macro porou anion exchang combin heat temperatur prevent precipit fatti acid present acid media ionic surfact remain effluent soap fatti acid separ extract ethanol anion surfact obtain ammonium salt elut aqueou isopropanol solut ammonium bicarbon ammonium salt degrad test cation surfact upset biodegrad test analyt procedur elimin cation exchang anion exchang chemic equip deionis water ethanol permiss denatur methyl ethyl keton methanol isopropanol water mixtur part volum isopropanol ch choh ch part volum water solut carbon dioxid ethanol approxim deliveri tube built sinter pass carbon dioxid ethanol ten minut fresh solut ammonium bicarbon solut mol nh hco ml isopropanol water mixtur consist part volum isopropanol part volum water cation exchang kat strongli acid resist alcohol mesh anion exchang aat macro porou merck lewatit mp mesh equival hydrochlor acid hcl ml round bottom flask ground glass stopper reflux condens mm diamet suction filter heatabl filter paper ml filter flask exchang column heat jacket tap tube mm diamet mm height figur water bath vacuum dry oven thermostat rotari evapor prepar extract separ anion activ agent prepar extract quantiti surfact biodegrad test mba quantiti product extract exce extract quantiti sampl practic reason quantiti product case limit prepar extract biodegrad test experi shown advantag number small extract larg extract exchang quantiti design work capac mmole surfact soap isol alcohol solubl constitu add synthet deterg analys ml ethanol heat mixtur boil point reflux hour stir pass hot alcohol solut coars pore suction filter heat filter rapidli wash flask suction filter approxim ml hot ethanol collect filtrat filter wash filter flask case past liquid product analys make anion surfact soap contain sampl evapor weigh sampl dryness dissolv residu ml ethanol proce case powder low appar densiti amp lt recommend increas ethanol ratio relat evapor ethanol filtrat dryness prefer mean rotari evapor repeat oper greater quantiti extract requir dissolv residu ml isopropanol water mixtur prepar ion exchang column cation exchang column place ml cation exchang resin ml beaker cover ad ml hydrochlor acid stand hour occasion stir decant acid transfer resin column mean deionis water column glass wool plug wash column deionis water rate ml min eluat free chlorid displac water ml isopropanol water mixtur rate ml min exchang column readi oper anion exchang column place ml anion exchang resin ml beaker cover ad ml deionis water resin swell hour transfer resin column mean deionis water column glass wool plug wash column ammonium bicarbon solut free chlorid requir ml solut wash ml deionis water displac water ml isopropanol water mixtur rate ml min exchang column form readi oper ion exchang procedur connect exchang column cation exchang column top anion exchang column heat exchang column thermostat control heat ml solut obtain item pass solut exchang combin rate ml min wash column ml hot isopropanol water mixtur obtain anion surfac activ agent mba disconnect kat column ml ethanol solut elut soap fatti acid kat column reject eluat elut mba aat column ml ammonium bicarbon solut evapor eluat dryness steam bath rotari evapor residu mba ammonium salt surfact anion detriment effect biodegrad test add deionis water residu definit volum obtain determin mba content aliquot solut standard solut anion synthet deterg biodegrad test solut temperatur regener ion exchang resin cation exchang reject pass addit quantiti ammonium bicarbon solut column flow rate approxim ml min eluat free anion surfact methylen blue test regener anion exchang resin pass ml isopropanol water mixtur anion exchang wash anion exchang readi oper preliminari treatment ionic surfact test preliminari note treatment sampl treatment ionic surfac activ agent formul deterg prior determin primari biodegrad confirmatori test purpos alcohol extract elimin insolubl inorgan ingredi commerci product circumst upset biodegrad test gt tabl gt ion exchang procedur isol separ ionic surfac activ agent soap anion cation surfact requir correct biodegrad test achiev ion exchang techniqu macro porou exchang resin suitabl eluant fraction elut soap anion ionic surfact isol procedur analyt control homogenis concentr anion ionic surfact deterg determin mba bia analyt procedur soap content determin suitabl analyt method analysi product calcul quantiti requir prepar fraction biodegrad test quantit extract ionic surfact extract obtain principl homogen sampl powder dri past dri liquid ethanol extract obtain surfact soap alcohol solubl constitu deterg sampl ethanol extract evapor dryness dissolv isopropanol water mixtur solut obtain pass strongli acid cation exchang macro porou anion exchang combin heat temperatur prevent precipit fatti acid present acid media ionic surfact obtain effluent evapor cation surfact upset degrad test analyt procedur elimin cation exchang anion exchang chemic equip deionis water ethanol permiss denatur methyl ethyl keton methanol isopropanol water mixtur part volum isopropanol ch choh ch part volum water ammonium bicarbon solut mol nh hco ml isopropanol water mixtur consist part volum isopropanol part volum water cation exchang kat strongli acid resist alcohol mesh anion exchang aat macro porou merck lewatit mp mesh equival hydrochlor acid hcl ml round bottom flask ground glass stopper reflux condens mm diamet suction filter heatabl filter paper ml filter flask exchang column heat jacket tap tube mm diamet mm height figur water bath vacuum dry oven thermostat rotari evapor prepar extract separ ionic activ agent prepar extract quantiti surfact degrad test bia prepar extract degrad test quantiti product limit maximum carri oper time order obtain suffici quantiti degrad test experi shown advantag number small extract larg extract isol alcohol solubl constitu add synthet deterg analys ml ethanol heat mixtur boil point reflux hour stir pass hot alcohol solut coars pore suction filter heat filter rapidli wash flask suction filter approxim ml hot ethanol collect filtrat filter wash filter flask case past liquid product analys make anion surfact soap contain sampl evapor weigh sampl dryness dissolv residu ml ethanol proce case powder low appar densiti amp lt recommend increas ethanol ratio relat evapor ethanol filtrat complet dryness prefer mean rotari evapor repeat oper greater quantiti extract requir dissolv residu ml isopropanol water mixtur prepar ion exchang column cation exchang column place ml cation exchang resin ml beaker cover ad ml hydrochlor acid stand hour occasion stir decant acid transfer resin column mean deionis water column glass wool plug wash column deionis water rate ml min eluat free chlorid displac water ml isopropanol water mixtur rate ml min exchang column readi oper anion exchang column place ml anion exchang resin beaker cover ad ml deionis water resin swell hour transfer resin column mean deionis water column glass wool plug wash column ammonium bicarbon solut free chlorid requir ml solut wash ml deionis water displac water ml isopropanol water mixtur rate ml min exchang column form readi oper ion exchang procedur connect exchang column cation exchang column top anion exchang column heat exchang column thermostat control heat ml solut obtain item pass solut exchang combin rate ml min wash column ml hot isopropanol water mixtur obtain ionic surfact collect filtrat filter wash evapor dryness prefer mean rotari evapor residu bia add deionis water defin volum obtain determin bia content aliquot solut standard solut ionic surfact degrad test solut temperatur regener ion exchang resin cation exchang reject pass ml ammonium bicarbon solut column flow rate approxim ml min eluat free anion surfact methylen blue test regener anion exchang resin pass ml isopropanol water mixtur anion exchang wash anion exchang readi oper figur activ sludg plant overview gt pic file quot en tif quot gt storag vessel dose devic aerat chamber litr capac settl vessel air lift pump collector sinter aerat air flow meter air figur activ sludg plant detail dimens millimetr gt pic file quot en tif quot gt liquid level hard pvc glass waterproof plastic hard pvc figur calcul biodegrad confirmatori test gt pic file quot en tif quot gt run period period calcul twenti dai readili biodegrad surfact surfact readili biodegrad biodegrad time dai figur heat exchang column dimens millimetr gt pic file quot en tif quot gt figur ga strip apparatu dimens millimetr gt pic file quot en tif quot gt