commiss decis fix criteria annual test breed poultri newcastl diseas applic articl council direct eec test eea relev ec commiss european commun regard treati establish european commun regard council direct eec octob anim health condit govern intra commun trade import countri poultri hatch egg amend council direct ec articl thereof methodolog perform serolog test newcastl diseas includ detail sampl procedur procedur carri test interpret test result measur provid decis accord opinion stand veterinari committe adopt decis articl serolog test detect newcastl diseas antibodi refer indent articl direct eec compli requir annex articl decis appli januari articl decis address member state brussel commiss rené steichen member commiss oj oj annex annual serolog test detect newcastl diseas antibodi poultri breed flock member state region member state vaccin statu sampl blood breed flock sampl annual basi blood sampl obtain bird randomli chosen examin haemagglutin inhibit test accord procedur list point procedur dispens ml pb well plastic microtitr plate bottom well place ml serum plate microtitr dilut make fold dilut serum plate add ml dilut allanto fluid hau mix tap place plate minimum minut room temperatur minimum minut add ml rbc well mix gentl tap place plate read minut control rbc settl tilt observ presenc absenc tear shape stream rate control well rbc ml pb ml titr highest dilut antiserum caus complet inhibiton unit viru ha titrat confirm presenc requir hau includ test valid result depend obtain titr hau hau neg control serum titr dilut titr posit control serum interpret test antigen affect level serum consid posit hau posit serum show titr greater hau posit serum show titr greater