commiss direct ec juli establish requir determin level dioxin dioxin pcb feedingstuff text eea relev commiss european commun regard treati establish european commun regard council direct eec juli introduct commun method sampl analysi offici control feedingstuff amend act access austria finland sweden articl thereof council direct ec april undesir substanc product anim nutrit amend direct ec establish maximum limit dioxin furan feed materi feedingstuff establish requir method analysi compli order ensur laboratori method analysi compar level perform provis sampl method analysi drawn basi present knowledg adapt account advanc scientif technolog knowledg provis laid direct relat analysi dioxin dioxin pcb implement direct ec amend direct ec undesir substanc product anim nutrit activ approach pursu order obtain comprehens reliabl data presenc dioxin pcb feed materi feedingstuff requir laid method analysi determin dioxin pcb feed materi feedingstuff screen method analysi proven wide accept valid high throughput select sampl signific level dioxin level dioxin sampl determin confirmatori method analysi establish requir confirmatori method analysi screen method measur provid direct accord opinion stand committe food chain anim health adopt direct articl member state ensur sampl offici control level dioxin furan determin level dioxin pcb feedingstuff carri accord method annex articl member state ensur sampl prepar method analys offici control level dioxin furan determin level dioxin pcb feedingstuff compli criteria annex ii articl member state bring forc law regul administr provis compli direct februari latest forthwith inform commiss thereof member state adopt provis refer direct accompani refer occas offici public member state determin refer made articl direct enter forc dai public offici journal european commun articl direct address member state brussel juli commiss david byrn member commiss oj oj oj annex method sampl offici control level dioxin pcdd pcdf determin dioxin pcb feedingstuff purpos scope sampl intend offici control level dioxin pcdd pcdf content determin content dioxin pcb feedingstuff provis commiss direct eec march establish commun method sampl offici control feedingstuff aggreg sampl obtain consid repres lot sublot complianc maximum level laid direct ec establish basi level determin laboratori sampl complianc lot sublot specif control laboratori analys laboratori sampl enforc duplic analysi case obtain result analysi maximum level calcul result lot accept result analysi maximum level duplic analysi conform respect maximum level laid direct ec tabl tef human risk assess base conclus world health organis meet stockholm sweden june van den berg al toxic equival factor tef pcb pcdd pcdf human wildlif environment health perspect gt tabl gt abbrevi tetra pe penta hx hexa hp hepta octa cdd chlorodibenzo dioxin cdf chlorodibenzofuran cb chlorobiphenyl oj annex ii sampl prepar requir method analysi offici control level dioxin pcdd pcdf determin dioxin pcb feedingstuff object field applic requir appli feed materi feedingstuff analys determin dioxin polychlorin dibenzo dioxin pcdd polychlorin dibenzofuran pcdf dioxin polychlorin biphenyl pcb monitor presenc dioxin feedingstuff perform strategi involv screen method order select sampl level dioxin dioxin pcb exce level interest concentr dioxin sampl signific level determin confirm confirmatori method screen method method detect presenc dioxin dioxin pcb level interest method capac high sampl throughput sift larg number sampl potenti posit specif design avoid fals neg confirmatori method method provid full complementari inform enabl dioxin dioxin pcb identifi quantifi unequivoc level interest background environment biolog sampl includ sampl feed materi feedingstuff gener complex mixtur dioxin congen concept toxic equival factor tef develop facilit risk assess tef establish express concentr mixtur substitut pcdd pcdf recent ortho mono ortho chlorin substitut pcb possess dioxin activ toxic equival teq tcdd annex footnot concentr individu substanc sampl multipli respect tef subsequ sum give total concentr dioxin compound express teq concept quot upperbound quot requir limit quantif contribut quantifi congen teq concept quot lowerbound quot requir contribut quantifi congen teq concept quot mediumbound quot requir half limit quantif calcul contribut quantifi congen teq qualiti assur requir compli sampl prepar gener provis prepar sampl analysi laid annex commiss direct eec juli amend direct eec eec eec eec eec eec eec eec establish commun method analysi offici control feedingstuff applic addit requir compli sampl store transport glass aluminium polypropylen polyethylen contain trace paper dust remov sampl contain glasswar rins solvent previous control presenc dioxin perform blank analysi carri entir analyt procedur omit sampl sampl weight extract suffici fulfil requir respect sensit requir laboratori laboratori demonstr perform method rang level interest level interest accept coeffici variat repeat analysi detail accept criteria point limit quantif confirmatori method rang level interest make accept coeffici variat met rang level interest regular blank control spike experi analysi control sampl prefer certifi refer materi perform intern qualiti control measur success particip interlaboratori studi assess laboratori profici prove compet specif analys success particip interlaboratori studi soil sewag sampl necessarili prove compet field food feedingstuff sampl present lower contamin level continu particip interlaboratori studi determin dioxin dioxin pcb relev feed food matric mandatori laboratori accredit recognis bodi oper accord iso guid ensur appli analyt qualiti assur laboratori accredit iso iec standard requir analyt procedur dioxin dioxin pcb basic requir accept analyt procedur high sensit low limit detect pcdd pcdf detect quantiti picogram teq rang extrem toxic compound pcb occur higher level pcdd pcdf pcb congen sensit nanogram rang suffici measur toxic dioxin pcb congen ortho substitut congen sensit reach pcdd pcdf high select specif distinct requir pcdd pcdf dioxin pcb multitud coextract possibl interf compound present concentr order magnitud higher analyt interest ga chromatographi mass spectrometri gc ms method differenti congen toxic seventeen substitut pcdd pcdf dioxin pcb congen bioassai determin teq valu select sum pcdd pcdf dioxin pcb high accuraci trueness precis determin provid valid reliabl estim true concentr sampl high accuraci accuraci measur close agreement result measur true assign measur avoid reject sampl analysi result basi poor reliabl estim teq accuraci express trueness differ measur analyt certifi materi certifi express percentag precis precis calcul standard deviat includ repeat reproduc close agreement result obtain appli experiment procedur time prescrib condit screen method compris bioassai gc ms method confirmatori method high resolut ga chromatographi high resolut mass spectrometri hrgc hrm method criteria compli total teq gt tabl gt specif requir gc ms method compli screen confirmatori purpos addit label chlorin substitut intern pcdd standard label intern dioxin pcb standard dioxin pcb determin carri begin start analyt method prior extract order valid analyt procedur congen tetra octa chlorin homolog group pcdd congen homolog group dioxin pcb dioxin pcb determin ad altern congen mass spectrometr select ion record function monitor pcdd dioxin pcb clear prefer case confirmatori method label substitut intern pcdd standard label intern dioxin pcb standard dioxin pcb determin rel respons factor determin congen label analogu ad calibr solut feedingstuff plant origin feedingstuff anim origin fat addit intern standard mandatori prior extract feedingstuff anim origin fat intern standard ad extract fat extract valid extract effici carri depend stage intern standard introduc result report product fat basi prior gc ms analysi recoveri surrog standard ad control recoveri confirmatori method recoveri individu intern standard rang lower higher recoveri individu congen hepta octa chlorin dibenzodioxin dibenzofuran accept condit contribut teq exce total teq base pcdd screen method recoveri rang separ dioxin interf chlorin compound pcb chlorin diphenyl ether carri suitabl chromatograph techniqu prefer florisil alumina carbon column gaschromatograph separ isom suffici amp lt peak peak hxcdf hxcdf determin perform epa method revis tetra octa chlorin dioxin furan isotop dilut hrgc hrm equival perform criteria differ upperbound level lower bound level exce feedingstuff dioxin contamin rang maximum level feedingstuff contamin level maximum level differ rang screen method analysi introduct analyt approach perform screen method pure screen approach quantit approach screen approach respons sampl compar refer sampl level interest sampl respons refer declar neg higher respons suspect posit requir blank refer sampl includ test seri extract test time ident condit refer sampl show elev respons comparison blank addit refer sampl level interest includ demonstr proper perform test rang interest control level interest test matric suitabl refer sampl demonstr preferenti includ sampl shown hrgc hrm teq level refer sampl blank spike level intern standard bioassai test repeat import obtain inform standard deviat test seri coeffici variat bioassai target compound interfer maximum toler blank level defin quantit approach quantit approach requir standard dilut seri duplic triplic clean measur blank recoveri control result express teq assum compound respons signal correspond teq principl perform tcdd dioxin furan standard mixtur produc calibr curv calcul teq level extract sampl subsequ correct teq level calcul blank sampl account impur solvent chemic recoveri calcul teq level qualiti control sampl limit interest essenti note part appar recoveri loss due matrix effect differ tef valu bioassai offici tef valu set requir method analysi screen gc ms method analysi bioassai screen gc ms method requir laid point cell base bioassai specif requir laid point kit base bioassai point inform number fals posit fals neg result larg set sampl maximum level action level comparison teq content determin confirmatori method analysi actual fals neg rate rate fals posit sampl low make screen tool advantag posit result confirm confirmatori method analysi hrgc hrm addit sampl wide teq rang confirm hrgc hrm approxim neg sampl inform correspond bioassai hrgc hrm result made specif requir cell base bioassai perform bioassai test run requir seri refer concentr tcdd dioxin furan mixtur full dose respons curv amp gt screen purpos expand low level curv analys low level sampl tcdd refer concentr time limit quantif qualiti control sheet outcom bioassai constant time period altern rel respons refer sampl comparison tcdd calibr line respons cell depend factor qualiti control qc chart type refer materi record check make outcom accord state guidelin quantit calcul induct sampl dilut linear portion respons curv sampl linear portion respons curv dilut test dilut time recommend test percent standard deviat triplic determin sampl dilut independ experi limit detect set time standard deviat solvent blank background respons approach appli respons background induct factor time solvent blank calcul calibr curv dai limit quantif set time standard deviat solvent blank background respons appli respons background induct factor time solvent blank calcul calibr curv dai specif requir kit base bioassai manufactur instruct sampl prepar analys test kit expir date materi compon design kit test kit rang storag temperatur oper temperatur limit detect immunoassai determin sum time standard deviat base replic analysi blank divid slope linear regress equat refer standard test laboratori make respons standard accept rang report result analyt procedur make analyt result level individu pcdd pcb congen report lowerbound upperbound mediumbound order includ maximum inform report result enabl interpret result specif requir report includ lipid content sampl method lipid extract recoveri individu intern standard made case recoveri rang mention point case maximum level exceed case request oj evid submit commerci kit base bioassai suffici sensit reliabl screen presenc dioxin requir level sampl foodstuff feedingstuff