commiss direct june amend annex council direct eec examin trichina trichinella spirali import countri fresh meat deriv domest swine eec commiss european commun regard treati establish european econom commun regard council direct eec decemb examin trichina trichinella spirali import countri fresh meat deriv domest swine amend direct eec articl thereof recent studi enabl method devis detect trichina pigmeat reliabl method point view health protect equival exist method addit made annex direct eec order facilit work examin trichina member countri member state permit choos examin method provid technic adapt made method examin trichina appli condit laboratori engag detect trichina compli measur laid direct accord opinion stand veterinari committe adopt direct articl direct eec amend set annex articl member state bring forc law regul administr provis compli direct januari forthwith inform commiss thereof articl direct address member state brussel june commiss poul dalsag member commiss oj oj annex annex amend point ii indent replac stereo microscop magnif suitabl light sourc indent replac digest liquid made pepsin fip fédération international de pharmaci ml hcl made litr tap water point iii replac iii method artifici digest collect sampl apparatu reagent knife tweezer collect specimen meat mincer mm diamet perfor litr erlenmey flask rubber cotton wool plug conic separ funnel ml capac ordinari base stand approxim cm length cm stem ring diamet approxim cm fix stand clamp flat vice mm attach stand mean doubl coupl siev mesh size micron extern diamet cm fit brass stainless steel mesh funnel intern diamet cm ml glass measur cylind stereo microscop magnif suitabl light sourc trichinoscop horizont tabl compressor suitabl light sourc trichinoscop larval count basin larval count basin made mm thick acryl plate bottom basin mm mark squar ii side mm iii end mm bottom end insert side form basin small handl end upper side bottom rais mm base frame form side end part fix glue materi number cm diamet petri dish stereo microscop mark undersid mm squar examin area point instrument number litr bin appli decontamin formol treatment apparatu remain digest juic case posit result concentr hydrochlor acid pepsin strength nf nation formulari bp british pharmacopoea fip fédération international de pharmaci number trai hold sampl approxim balanc accur collect specimen case carcas specimen approxim pillar diaphragm transit sinewi part absenc diaphragm pillar specimen size rib part breastbon part diaphragm lingual muscl jaw muscl abdomin muscl cut meat sampl approxim skelet muscl fat bone tendon method complet pool sampl time approxim sampl individu sampl pig pool sampl put mincer minc meat litr erlenmey flask pepsin approxim litr tap water heat ml concentr hydrochlor acid mixtur shaken dissolv pepsin ph solut digest erlenmey flask incub approxim hour flask regularli shaken time incub hour digest solut filter siev conic litr separ funnel left undisturb stand hour total volum approxim ml run measur cylind divid petri dish bottom mark squar ml dish petri dish minut examin larval trichina stereo microscop larval count basin emploi ml distribut larval count basin examin trichinoscop larva identifi organ deposit water lukewarm roll unrol movement quot spiral quot observ digest examin readi circumst examin postpon dai digest unclear examin minut prepar clarifi final sampl ml pour measur cylind allow stand minut end time ml supernat fluid remov suction remain ml made ml tap water settl period minut ml supernat fluid remov suction remain ml pour petri dish larval count basin examin measur cylind wash ml tap water wash ad sampl petri dish larval count basin examin ii pool sampl singl sampl ad total pool sampl examin sampl sampl sampl examin digest fluid reduc proportion case posit doubt result examin collect sampl sampl pig accord sampl pig pool examin method sampl group pig examin trichinella detect pool sampl pig sampl collect individu pig group separ examin method point iv vi ad iv mechan assist pool sampl digest method sediment techniqu apparatu reagent knife scissor cut specimen trai mark squar hold sampl approxim meat stomach lab blender thermo model plastic bag suitabl stomach lab blender conic separ funnel litr capac prefer fit teflon safeti plug stand ring clamp siev mesh size micron extern diamet cm stainless steel mesh funnel intern diamet cm support siev ml glass measur cylind ml dispens beaker litr capac spoon glass rod stir digest fluid beaker plastic syring tube suction measur spoon thermomet accur rang vibrat electr shaver head remov relai switch minut interv trichinoscop horizont tabl stereo microscop suitabl light sourc larval count basin trichinoscop larval count basin made mm thick acryl plate bottom basin mm mark squar ii side mm iii end mm bottom end insert side form small handl end upper side bottom rais mm base frame form side end part fix glue materi number cm diamet petri dish stereo microscop mark undersid mm squar examin area point instrument hydrochlor acid solut pepsin strength nf nation formulari bp british pharmacopoea fip fédération international de pharmaci number litr bin appli decontamin formol treatment apparatu remain digest juic case posit result balanc accur collect specimen case carcas specimen approxim pillar diaphragm transit sinewi part absenc diaphragm pillar specimen size rib part breastbon part diaphragm jaw muscl abdomin muscl cut meat sampl approxim skelet muscl fat bone tendon method digest procedur complet pool sampl time stomach lab blender fit doubl plastic bag temperatur control set half litr water preheat pour plastic bag water heat ml hydrochlor acid ad water stomach sampl approxim individu sampl accord ad pepsin final ad order addit strictli adher order avoid decomposit pepsin stomach allow pound content bag minut pastic bag remov stomach digest fluid filter siev litr beaker plastic bag wash approxim ml water rins siev final ad filtrat beaker singl sampl ad total pool sampl examin sampl ii smaller pool sampl stomach lab blender fit doubl plastic bag temperatur control set digest fluid prepar mix half litr water ml hydrochlor acid pepsin ad mix temperatur order addit strictli adher order avoid decomposit pepsin digest fluid volum ml gram sampl measur sampl volum requir ml ml transfer plastic bag meat sampl approxim individu sampl accord water temperatur approxim pour outer bag total volum bag half litr stomach allow pound content bag minut plastic bag remov stomach digest fluid filter siev litr beaker plastic bag wash approxim ml water rins siev final ad filtrat beaker recoveri larva sediment ic ic flake scali ic crush ic ad digest fluid bring volum litr digest fluid stir ic melt case smaller pool ii amount ic reduc correspondingli chill digest fluid transfer litr separ funnel equip vibrat extra clamp sediment minut time sediment funnel vibrat intermitt minut vibrat minut paus minut ml sampl sediment quickli run ml measur cylind funnel rins deterg solut ml sampl allow stand minut time supernat withdrawn suction leav volum ml examin presenc larva suction dispos syring equip plastic tube length tube ml remain measur cylind flang syring rest cylind rim remain ml pour larval count basin petri dish examin trichinoscop stereo microscop digest examin readi circumst examin postpon dai digest unclear examin minut prepar clarifi final sampl ml pour measur cylind allow stand minut end time ml supernat fluid remov suction remain ml made ml tap water settl period minut ml supernat fluid remov suction remain ml pour petri dish larval count basin examin measur cylind wash ml tap water wash ad sampl petri dish larval count basin examin case posit doubt result examin collect sampl sampl pig accord sampl pig pool examin method sampl group pig examin trichinella detect pool sampl pig sampl collect individu pig group separ examin method mechan assist pool sampl digest method quot filter isol quot techniqu apparatu reagent method iv supplementari equip abovement litr gelman funnel complet filter holder diamet mm filter disc filter disc consist circular stainless steel mesh apertur micron diamet disc mm rubber ring made mm thick rubber extern diamet mm intern diamet mm circular mesh rubber ring bond compon glue suitabl materi erlenmey flask capac litr fit side tube suction filter pump plastic bag capac ml equip seal plastic bag rennilas strength soxhlet unit gram collect specimen method iv method digest procedur complet pool sampl time method iv ii smaller pool sampl method iv ii recoveri larva filtrat ic ic flake scali ic crush ic ad digest fluid bring volum litr case smaller pool amount ic reduc correspondingli digest fluid stir ic melt chill digest fluid left minut larva coil gelman funnel fit filter holder filter disc mount erlenymey flask connect filter pump digest fluid pour gelman funnel filter end filtrat passag digest fluid filter assist appli suction filter pump suction ceas filter dry ml fluid left funnel digest fluid filter filter disc remov ml capac plastic bag ml rennilas solut solut rennilas made ad rennilas ml tap water plastic bag seal stomach outer bag stomach allow pound minut work complet incomplet pool minut plastic bag complet filter disc rennilas solut remov stomach open scissor liquid content pour larval count basin petri dish bag wash ml water ad larval count basin examin trichinoscop petri dish examin stereo microscop digest examin readi circumst examin postpon dai note filter disc complet clean unclean disc allow dry filter disc clean leav rennilas solut overnight wash fresh rennilas solut stomach case posit doubt result examin collect sampl sampl pig accord sampl pig pool examin method sampl group pig examin trichinella detect pool sampl pig sampl collect individu pig group separ examin method vi magnet stirrer method pool sampl digest apparatu reagent knife tweezer cut specimen trai mark squar hold sampl approxim meat moulinett blender magnet stirrer thermostat control heat plate teflon coat stir rod approxim cm long conic separ funnel litr capac stand ring clamp siev mesh size micron extern diamet cm stainless steel mesh funnel intern diamet cm support siev beaker litr capac measur cylind approxim ml capac centrifug tube trichinoscop horizont tabl stereo microscop suitabl light sourc larval count basin trichinoscop larval count basin made mm thick acryl plate bottom basin mm mark squar ii side mm iii end mm bottom end insert side form small handl end upper side bottom rais mm base frame form side end part fix glue materi number cm diamet petri dish stereo microscop mark undersid mm squar examin area point instrument aluminium foil hydrochlor acid pepsin strength nf nation formulari bp british pharmacopoea fip fédération international de pharmaci tap water heat number litr bin appli decontamin formol treatment apparatu remain digest juic case posit result balanc accur collect specimen case carcas specimen approxim pillar diaphragm transit sinewi part absenc diaphragm pillar specimen size rib part breastbon part diaphragm jaw muscl abdomin muscl cut meat sampl approxim skelet muscl fat bone tendon method complet pool sampl time sampl approxim individu sampl accord chop moulinett blender blender oper time approxim time chop meat transfer litr beaker sprinkl pepsin litr tap water preheat pour beaker ml hydrochlor acid minc insert moulinett blender immers repeatedli digest fluid beaker remov meat adher stir rod beaker beaker cover aluminium foil beaker preheat heat plate magnet stirrer stir process start start stir process magnet stirrer adjust maintain constant temperatur period oper stir process digest fluid rotat suffici high speed creat deep whirl splash digest fluid stir minut end stirrer switch digest fluid pour siev sediment funnel digest fluid allow stand funnel minut minut ml sampl digest fluid quickli run measur cylind centrifug tube ml sampl allow stand minut end time ml supernat withdrawn suction leav volum ml remain ml sampl sediment pour larval count basin petri dish cylind centrifug tube rins ml tap water ad sampl larval count basin petri dish subsequ sampl examin trichinoscop stereo microscop digest examin readi circumst examin postpon dai digest examin minut prepar clarifi final sampl ml pour measur cylind allow stand minut time ml supernat fluid remov leav volum ml volum made ml tap water settl period minut ml supernat fluid withdrawn suction leav volum ml examin petri dish larval count basin measur cylind wash ml tap water wash ad sampl petri dish larval count basin examin sediment found unclear examin sampl pour measur cylind made ml tap water procedur ii pool sampl need sampl ad total pool sampl examin sampl sampl examin complet pool pool sampl digest fluid reduc litr case posit doubt result examin collect sampl sampl pig accord sampl pig pool examin method sampl group pig examin trichinella detect pool sampl pig sampl collect individu pig group separ examin method annex ii chapter paragraph amend text point replac adequ equip lockabl examin room darken examin perform trichinoscop point delet point text point replac washroom cleans disinfect examin equip contain sampl compressor knive scissor waterproof floor cover rot proof easi clean disinfect smooth wall height line washabl light colour cover paint provis appli method point ii iii iv vi annex provid laboratori equip larg suitabl plumb sink