council direct eec introduc commun measur control avian influenza council european commun regard treati establish european econom commun articl thereof regard propos commiss regard opinion european parliament regard opinion econom social committe poultri list annex ii treati market poultri constitut import sourc revenu agricultur popul establish commun level control measur event outbreak highli pathogen form avian influenza caus influenza viru specif characterist hereinaft term avian influenza order ensur nation develop poultri sector contribut protect anim health commun outbreak avian influenza quickli epizoot proport caus mortal disturb scale liabl reduc sharpli profit farm poultri action presenc diseas suspect effect control measur implement presenc confirm prevent spread diseas outbreak occur carefulli monitor movement anim product liabl contamin vaccin diagnosi diseas carri auspic respons nation laboratori coordin ensur commun refer laboratori common measur control avian influenza form basi maintain unifi standard relat anim health articl council decis eec june expenditur veterinari field appli event occurr avian influenza confer commiss task take applicatori measur adopt direct articl direct defin commun control measur appli event outbreak avian influenza poultri prejudic commun provis govern intra commun trade direct appli avian influenza detect bird case member state concern inform commiss measur take articl purpos direct definit articl council direct eec octob anim health condit govern intra commun trade import countri poultri hatch egg appli definit appli infect poultri poultri presenc avian influenza mean annex offici confirm examin approv laboratori case subsequ outbreak clinic sign post mortem lesion consist avian influenza present poultri suspect infect poultri show clinic sign post mortem lesion presenc avian influenza suspect poultri presenc influenza viru subtyp demonstr poultri suspect contamin poultri directli indirectli expos avian influenza viru influenza viru subtyp subtyp compet author compet author mean articl direct eec offici veterinarian veterinarian design compet author articl member state ensur compulsori notif suspect presenc avian influenza compet author articl poultri hold suspect infect contamin avian influenza member state ensur offici veterinarian immedi activ offici investig arrang confirm rule presenc diseas sampl laboratori examin suspect infect notifi compet author hold offici surveil requir record made categori poultri hold show respect categori number poultri di show clinic sign show sign record date includ bird born dy period suspicion data record date produc request check visit poultri hold live quarter confin place isol contact poultri poultri enter leav hold movement person anim vehicl hold poultri meat carcas anim feed implement wast drop manur litter liabl transmit avian influenza subject author compet author egg leav hold except egg directli establish approv manufactur process egg product articl direct eec transport author grant compet author author meet requir laid annex mean disinfect entranc exit build hous poultri hold epizootiolog inquiri carri accord articl time offici measur laid paragraph enforc owner keeper poultri diseas suspect reason action ensur complianc paragraph thereof compet author appli measur provid paragraph hold locat configur contact hold disesas suspect give reason suspect contamin measur refer paragraph withdrawn suspicion avian influenza rule offici veterinarian articl presenc avian influenza offici confirm hold member state ensur compet author requir addit measur list articl measur undertaken poultri hold delai kill spot poultri di kill egg destroi oper carri minim risk spread diseas substanc wast anim feed litter manur liabl contamin destroi treat appropri treatment carri accord instruct offici veterinarian ensur destruct avian influenza viru present poultri hold slaughter presum incub period diseas meat poultri trace destroi hatch egg laid presum incub period move hold trace destroi poultri hatch egg offici surveil tabl egg laid presum incub period move hold whereev trace destroi previous properli disinfect carri oper list subparagraph build hous poultri surround vehicl transport equip contamin clean disinfesct accord provis articl poultri reintroduc hold dai complet oper provid subparagraph epizootiolog inquiri carri accord articl compet author extend measur provid paragraph neighbour hold locat configur contact hold diseas confirm give reason suspect contamin articl case hold consist separ flock compet author accord criteria set commiss procedur laid articl grant derog requir articl healthi flock hold infect provid offici veterinarian confirm oper carri flock complet separ hous keep feed viru spread flock articl epizootiolog inquiri deal length time avian influenza exist hold origin avian influenza hold identif hold poultri infect contamin sourc movement person poultri anim vehicl egg meat carcas implement substanc carri avian influenza viru hold question order provid full coordin measur ensur erad avian influenza quickli purpos carri epidemiolog inquiri crisi unit establish gener rule nation crisi unit commun crisi unit laid council act qualifi major propos commiss articl offici veterinarian reason suspect poultri hold contamin result movement person anim vehicl hold offici control accord paragraph purpos offici control detect immedi suspicion avian influenza count poultri monitor movement action provid paragraph hold subject offici control paragraph compet author prohibit remov poultri hold transport directli slaughterhous offici supervis purpos slaughter grant author offici veterinarian carri clinic examin poultri exclud presenc avian influenza hold movement restrict refer articl impos period dai latest date potenti contamin restrict appli period dai consid condit permit compet author limit measur provid articl part hold poultri contain provid poultri hous fed complet separ separ staff articl diagnosi avian influenza offici confirm member state ensur compet author establish infect hold protect zone base minimum radiu kilometr contain surveil zone base minimum radiu kilometr establish zone account geograph administr ecolog epizootiolog factor relat avian influenza monitor facil measur appli protect zone includ identif hold poultri whithin zone period visit hold poultri clinic examin poultri includ collect sampl laboratori examin record visit find keep poultri live quarter place isol mean disinfect entranc exit hold control movement person handl poultri poultri carcas egg vehicl carri poultri carcas egg zone gener transport poultri prohibit transit major highwai railwai prohibit remov poultri hatch egg hold compet author author transport poultri slaughter slaughterhous prefer locat infect area slaughterhous design compet author infect area special health mark provid articl direct eec appli poultrymeat ii dai chick readi lai pullet hold surveil zone poultri hold offici control provid articl iii hatch egg hatcheri design compet author dispatch egg pack disinfect movement allow ii iii directli execut offici control author offici veterinarian carri health inspect hold mean transport clean disinfect prohibit remov spread litter poultri manur author prohibit fair market show gather poultri bird measur appli protect zone maintain dai carri preliminari clean disinfect oper infect hold accord articl protect zone part surveil zone measur appli surveil zone includ identif hold poultri zone control poultri hatch egg movement zone prohibit movement poultri zone dai movement directli slaughterhous surveil zone design compet author special health mark provid articl direct eec appli poultri meat prohibit movement hatch egg surveil zone hatcheri design compet author dispatch egg pack disinfect prohibit movement litter poultri manur zone prohibit fair market show gather poultri bird prejudic provis prohibit transport poultri transit major highwai railwai measur appli surveil zone maintain dai carri preliminari clean disinfect oper infect hold accord articl zone situat territori member state compet author member state concern cooper establish area paragraph protect zone surveil zone establish procedur provid articl articl member state ensur compet author determin arrang allow trace movement egg poultri owner keeper poultri requir suppli compet author respons request author inform poultri egg enter leav hold person engag transport market poultri egg suppli compet author inform movement poultri egg transport market furnish detail inform articl member state ensur disinfect concentr offici approv compet author clean disinfect oper carri offici supervis accord instruct offici veterinarian ii procedur clean disinfect infect hold laid annex ii articl collect sampl laboratori test detect presenc avian influenza viru carri accord annex iii articl member state ensur compet author take measur person establish protect surveil zone inform restrict forc make arrang implement measur question articl member state ensur member state design nation laboratori facil expert personnel maintain permit assess pathogen influenza viru isol accord annex iii chapter identif influenza virus subtyp nation laboratori reagent region laboratori test nation institut laboratori author vaccin test order verifi conform specif laid market author nation laboratori list annex iv respons coordin standard method diagnosi reagent test vaccin nation laboratori list annex iv respons coordin standard diagnost method laid avian influenza diagnost laboratori member state end provid diagnost reagent nation laboratori control qualiti diagnost reagent member state arrang compar test period hold isol avian influenza viru case confirm member state ensur confirm posit result obtain region diagnost laboratori nation laboratori list annex iv liais commun refer laboratori refer articl articl commun refer laboratori avian influenza mention annex prejudic provis decis eec articl thereof power duti laboratori appear annex articl vaccin avian influenza vaccin author compet author supplement control measur carri diseas appear accord provis decis introduc vaccin supplement control measur commiss collabor member state concern act accord procedur laid articl decis regard concentr poultri affect area characterist composit vaccin procedur supervis distribut storag vaccin speci categori poultri subject vaccin area vaccin carri derog subparagraph decis introduc emerg vaccin outbreak member state concern notif commiss provid fundament interest commun jeopard decis examin immedi stand veterinari committe accord procedur provid articl member state author accord point recours emerg vaccin limit part territori statu remaind territori affect provid immobil measur vaccin anim effect period determin accord procedur laid articl articl member state draw conting plan nation measur implement event outbreak avian influenza plan access facil equip personnel materi rapid effici erad outbreak criteria appli draw plan laid annex vi plan drawn accord criteria list annex vi submit commiss month direct brought applic commiss examin plan order determin permit desir object attain suggest member state concern amend requir ensur compat member state commiss approv plan amend accord procedur laid articl plan subsequ amend supplement accord procedur account develop situat articl commiss expert collabor compet author ensur uniform applic direct make spot check order check repres percentag establish compet author check establish fulfil requir direct commiss inform member state result check carri member state territori check carri give assist expert carri duti gener provis implement articl determin accord procedur laid articl articl detail condit govern commun financi contribut measur connect applic direct laid decis eec articl annex amend requir council act qualifi major act propos commiss order account develop research diagnost procedur articl procedur laid articl stand veterinari committe set decis eec hereinaft refer committe inform delai chairman initi request repres member state repres commiss submit committe draft time limit chairman lai urgenc matter opinion deliv major laid articl treati case decis council requir adopt propos commiss vote repres member state committe weight manner set articl chairman vote commiss adopt measur envisag accord opinion committe measur envisag accord opinion committe opinion deliv commiss delai submit council propos relat measur council act qualifi major expiri period month date referr council council act propos measur adopt commiss save council decid measur simpl major articl member state bring forc law regul administr provis compli direct januari forthwith inform commiss thereof member state adopt measur refer direct accompani refer occas offici public method make refer laid member state articl direct address member state brussel council presid arlindo marqu cunha oj oj oj oj decis amend decis eec oj oj direct amend direct eec oj council direct eec june oj amend direct eec oj oj direct amend direct eec oj oj oj annex author remov egg hold subject condit articl direct author issu compet author transport egg suspect hold subject provis articl establish approv manufactur process egg product accord provis articl direct eec hereinaft call design establish meet condit order allow remov suspect undertak egg compli requir laid chapter iv annex direct eec directli suspect undertak design establish consign seal dispatch offici veterinarian suspect hold remain seal transport design establish offici veterinarian suspect undertak inform compet author design establish intent send egg compet author respons design establish ensur egg refer isol egg time arriv process shell egg regard high risk materi accord articl direct eec dealt accord requir chapter ii direct packag materi vehicl transport egg refer premis egg contact clean disinfect destroi avian influenza viru offici veterinarian suspect hold inform consign process egg oj annex ii procedur clean disinfect infect hold preliminari clean disinfect carcas poultri remov dispos part premis poultri hous part build yard contamin slaughter post mortem examin sprai disinfect approv accord articl direct tissu poultri egg contamin build yard utensil carefulli collect dispos carcas disinfect remain surfac hour ii final clean disinfect greas dirt remov surfac applic degreas agent wash water wash water sprai disinfect appli dai premis treat degreas agent rins cold water sprai disinfect rins water litter manur treat method capabl kill viru method compris practic inciner steam treatment temperatur ii buri deep prevent access vermin wild bird iii stack dampen facilit ferment cover heat temperatur attain leav cover dai prevent access vermin wild bird annex iii diagnost procedur confirm differenti diagnosi avian influenza ai procedur isol character avian influenza virus regard guidelin minima appli diagnosi diseas purpos diagnost procedur confirm differenti diagnosi avian influenza definit appli avian influenza mean infect poultri caus influenza viru intraven pathogen index week chicken greater infect influenza virus subtyp nucleotid sequenc demonstr presenc multipl basic amino acid cleavag site haemagglutinin chapter sampl treatment sampl sampl cloacal swab faec tracheal swab sick bird faec intestin content brain tissu trachea lung liver spleen affect organ recent dead bird treatment sampl organ tissu list paragraph pool separ treatment faecal materi essenti swab suffici antibiot medium ensur full immers faec sampl organ homogen enclos blender pestl mortar steril sand antibiot medium made suspens medium suspens left hour ambient temperatur longer period clarifi centrifug minut antibiot medium laboratori formul antibiot medium success nation laboratori offer advic countri high concentr antibiot requir faec sampl typic mixtur unit ml penicillin mg ml streptomycin mg ml gentamycin unit ml mycostatin phosphat buffer salin level reduc fold tissu tracheal swab control chlamydia organ mg ml oxytetracyclin ad imper make medium ph check addit antibiot readjust ph chapter viru isol viru isol embryon fowl egg clarifi supernat fluid inocul ml amount allanto caviti minimum embryon fowl egg incub dai ideal egg obtain specif pathogen free flock impractic accept egg obtain flock shown free antibodi avian influenza inocul egg held candl daili egg dead dy embryo aris remain egg dai inocul chill allanto amniot fluid test haemagglutin activ haemagglutin detect procedur repeat undilut allanto amniot fluid inoculum haemagglutin detect presenc bacteria exclud cultur bacteria present fluid pass nm membran filter antibiot ad inocul embryon egg chapter differenti diagnosi preliminari differenti import control measur aim limit spread viru implement region laboratori posit identifi isol haemagglutin viru influenza virus subtyp addit newcastl diseas viru haemagglutin fluid haemagglutin inhibit test chapter posit inhibit amp polyclon antisera specif subtyp influenza titr serv preliminari identif enabl imposit interim control measur confirmatori identif haemagglutinin subtyp neuraminidas subtyp influenza virus variat occur practic cost effect nation laboratori hold antisera full antigen character influenza isol nation laboratori confirm isol influenza viru immunodoublediffus test detect group antigen chapter immunofluoresc elisa techniqu detect group antigen prefer nation laboratori ii determin isol subtyp iii carri intraven pathogen index test week chicken chapter intraven pathogen indic greater presenc viru requir full implement control measur exercis nation laboratori carri test determin capac isol produc plaqu cell cultur chapter nation laboratori immedi submit avian influenza isol commun refer laboratori full character type character isol commun refer laboratori receiv haemagglutin virus nation laboratori antigen genet studi enabl greater understand epizootiolog diseas european commun keep function duti refer laboratori addit duti commun refer laboratori carri full antigen type influenza virus receiv virus intraven pathogen indic greater nucleotid sequenc haemagglutinin gene determin multipl basic amino acid cleavag site haemagglutinin protein carri chapter seriolog test avian influenza viru antibodi erad programm subtyp viru respons homolog viru antigen serolog monitor evid infect haemagglutin inhibit test chapter haemagglutinin subtyp evid infect influenza virus obtain detect antibodi direct group specif antigen purpos immunodoublediffus test chapter elisa test problem elisa host specif test depend detect host immunoglobulin waterfowl rare give posit result immunodoublediffus test subtyp practic examin bird presenc antibodi subtyp sampl blood sampl bird flock size bird larger flock give probabl detect posit serum flock posit flock size blood allow clot serum remov test examin antibodi individu serum sampl test abil inhibit influenza viru haemagglutin antigen standard haemagglutin inhibit test defin chapter debat haemagglutinin unit test valid choic left discret nation laboratori antigen affect level serum consid posit hau posit serum show titr amp greater hau posit serum show titr greater chapter haemagglutin ha test reagent isoton salin buffer phosphat ph red blood cell rbc pool minimum specif pathogen free chicken blood bird regularli monitor shown free avian influenza antibodi equal volum alsev solut cell wash time pb test suspens pack cell pb recommend commun refer laboratori suppli recommend virus low virul standard antigen procedur dispens ml pb plastic microtitr plate bottom well place ml viru suspens allanto fluid microtitr dilut make fold dilut viru plate dispens ml pb add ml red blood cell mix tap gentli place plate read minut red blood cell control settl read tilt plate observ presenc absenc tear shape stream rbc well ha flow rate control cell viru ha titr highest dilut agglutin rbc dilut regard ha unit hau accur method determin ha titr ha test viru close rang initi dilut recommend accur prepar antigen haemagglutin inhibit test chapter chapter haemagglutin inhibit hl test reagent phosphat buffer solut pb viru allanto fluid dilut pb hau ml chicken rbc neg control chicken serum posit control serum procedur dispens ml pb well plastic microtitr plate bottom well place ml serum plate microtitr dilut make fold dilut serum plate add ml dilut allanto fluid hau mix tap place plate minimum minut room temperatur minimum minut add ml rbc well mix gentl tap place plate read minut control rbc settl tilt observ presenc absenc tear shape stream rate control well rbc ml pb ml hl titr highest dilut antiserum caus complet inhibit unit viru ha titrat confirm presenc requir hau includ test valid result depend obtain titr hau hau neg control serum titr dilut titr posit control serum chapter intraven pathogen index ivpi infect allanto fluid lowest passag level prefer initi isol select dilut steril isoton salin ml dilut viru inject intraven week chicken specif pathogen free bird bird examin hour interv dai observ bird record normal sick sever sick dead record result calcul index shown clinic sign dai inocul total score normal sick sever sick dead total index score bird observ subject clinic judgment involv bird show sign respiratori involv depress diarrhoea cyanosi expos skin wattl oedema face head nervou sign chapter assess plaqu form abil dilut rang viru ensur optimum number plaqu present plate ten fold dilut amp pb suffici confluent monolay chick embryo cell suitabl cell line madin darbi bovin kidnei prepar cm diamet petri dish ml viru dilut ad petri dish viru allow absorb minut wash time pb infect cell overlaid relev medium agar mg ml trypsin trypsin import serum ad overlai medium hour incub plaqu suffici size remov agar overlai stain cell monolay crystal violet ethanol virus give clear plaqu incub presenc trypsin overlai trypsin absent overlai virus virul chicken produc plaqu chapter immunodoublediffus prefer method show presenc influenza viru demonstr possess nucleocapsid matrix antigen share influenza viru gener immunodoublediffus test involv concentr viru prepar extract infect chorioallanto membran suitabl prepar concentr viru made simpl high speed centrifug infecti allanto fluid disrupt viru releas intern nucleocapsid matrix antigen treatment deterg sodium lauroyl sarcosin acid precipat ad hcl infect allanto fluid give final ph chill hour low speed centrifug minut supernat discard viru precipit resuspend minimum volum glycin sarkosyl buffer sodium lauroyl sarcosin buffer ph glycin prepar possess nucleocapsid matrix antigen beard prepar nucleocapsid rich antigen chorioallanto membran remov infect egg method involv remov chorioallanto membran infect haemagglutinin posit egg grind homogenis membran freez thaw time centrifug minut pellet discard supernat treat formalin antigen antigen immunodoublediffus test agaros agar gel sodium chlorid made phosphat buffer ph influenza viru confirm precipitin line form test antigen posit antigen posit antiserum coalesc give line ident annex iv list nation avian influenza laboratori belgium institut nation de recherch vétérinair groeselenberg brussel denmark nation veterinari laboratori poultri diseas divis hangoevej dk aarhu germani institut fuer kleintierzucht der bundesforschungsanstalt fuer landwirtschaft braunschweig voelkenrod postfach cell franc centr nation etud vétérinair alimentair laboratoir central de recherch avicol porcin ploufragan greec ëïéìùaeþí íïóçìueôùí ïêôùâñssïõ gr èaaóóáëïíssêç institut infect parasitolog diseas octob street thessaloniki ireland veterinari research laboratori abbotstown castleknock dublin itali istituto patologi aviair facoltà di medicina veterinaria università di napoli aniezzo falcon napoli delpino luxembourg institut nation de recherch vétérinair groeselenberg brussel netherland centraal diergeneeskundig instituut vestig virologi houtribweg nl ra lelystad portug laboratório nacion de investigaçao veterinária lniv estrada de benfica lisbon spain centro nacion de referencia para la pest aviar es el laboratorio nacion de sanidad producción anim de barcelona zona franca circunvalación tramo esquina call barcelona unit kingdom central veterinari laboratori haw uk weybridg surrei kt nb annex commun refer laboratori avian influenza laboratori central veterinari laboratori haw uk weybridg surrei kt nb unit kingdom function duti ec refer laboratori avian influenza coordin consult ec commiss method emploi member state diagnos avian influenza specif type store suppli strain avian influenza viru serolog test prepar antisera suppli standard sera refer reagent nation refer laboratori order standard test reagent member state build retain collect avian influenza viru strain isol organ period compar test diagnost procedur commun level collect collat data inform method diagnosi result test carri commun character isol avian influenza virus date method greater understand epizootiolog avian influenza gain insight epizootiolog viru emerg highli pathogen potenti pathogen strain keep abreast develop avian influenza surveil epizootiolog prevent world retain expertis avian influenza viru pertin virus enabl rapid differenti diagnosi acquir knowledg prepar product veterinari immunolog erad control avian influenza activ assist diagnosi avian influenza outbreak member state receiv viru isol confirmatori diagnosi character epizootiolog studi laboratori carri nucleotid sequenc analysi determin deduc amino acid sequenc cleavag site haemagglutin molecul avian influenza virus subtpy facilit train retrain expert laboratori diagnosi view harmon techniqu commun annex vi criteria conting plan conting plan meet criteria establish crisi centr nation level coordin control measur member state concern list provid local diseas control centr adequ facil coordin diseas control measur local level detail inform staff involv control measur skill respons local diseas control centr contact rapidli person organ directli indirectli involv outbreak equip materi carri diseas control measur properli detail instruct provid action suspicion confirm infect contamin includ propos mean dispos carcas train programm establish maintain develop skill field administr procedur diagnost laboratori facil post mortem examin capac serolog histolog maintain skill rapid diagnosi arrang made rapid transport sampl detail provid quantiti avian influenza vaccin estim requir event reinstat emerg vaccin provis made ensur legal power implement conting plan