commiss decis approv african swine fever diagnost manual notifi document number text eea relev ec commiss european commun regard treati establish european commun regard council direct ec june lai specif provis control african swine fever amend direct eec teschen diseas african swine fever articl thereof pursuant direct ec lai uniform diagnost procedur sampl method criteria evalu result laboratori test confirm african swine fever accord direct commun refer laboratori african swine fever coordin consult commiss method emploi member state diagnos diseas organis inter alia period compar test suppli standard reagent commun level african swine fever viru consid hazard human health laboratori test develop ensur rapid confirm african swine fever experi gain control african swine fever recent year result identif suitabl sampl procedur criteria evalu laboratori test result proper diagnosi diseas situat approv manual lai procedur criteria nation diagnost laboratori authoris modifi approv laboratori test test provid equal sensit specif demonstr measur provid decis accord opinion stand committe food chain anim health adopt decis articl african swine fever diagnost manual annex approv member state ensur confirm african swine fever carri accord procedur sampl method criteria evalu laboratori test result laid manual base detect clinic sign post mortem lesion diseas detect viru antigen genom sampl pig tissu organ blood excreta demonstr specif antibodi respons blood sampl derog paragraph nation diagnost laboratori refer annex iv direct ec appli modif laboratori test refer manual test provid equal sensit specif demonstr modifi test appli sensit specif evalu framework period compar test organis commun refer laboratori african swine fever articl decis appli juli articl decis address member state brussel commiss david byrn member commiss oj annex african swine fever diagnost manual chapter introduct object definit order ensur uniform procedur diagnos african swine fever hereinaft quot asf quot manual guidelin minimum requir diagnost procedur sampl method criteria evalu result clinic post mortem examin laboratori test correct diagnosi asf establish minimum bio safeti requir qualiti standard observ asf diagnost laboratori transport sampl establish laboratori test diagnosi asf laboratori techniqu genet type asf viru isol manual princip direct author respons control asf emphasi principl applic laboratori test evalu result detail laboratori techniqu purpos manual addit definit refer articl direct ec definit appli quot suspect hold quot mean pig hold pig suspect infect asf viru contact hold defin articl direct ec quot epidemiolog subunit quot quot subunit quot mean build place land nearbi group pig hold frequent direct indirect contact time separ pig hold quot contact pig quot mean pig live hold direct contact pig suspect infect asf viru previou dai chapter ii descript asf emphasi differenti diagnosi introduct asf caus envelop dna viru belong genu asfiviru famili asfarvirida asf viru strain differ virul serotyp identifi asf viru stabl excret infect pig pig carcas fresh pig meat pig meat product disinfect ensur inactiv environ main natur rout infect pig europ oro nasal direct indirect contact infect pig feed viru contamin feed area vector exist transmiss vector plai import role viru persist spread asf spread indirect contact contamin materi bite insect mechan transport asf viru diseas transmiss occur semen infect boar incub period individu anim dai field condit clinic symptom evid hold week viru introduct mild strain viru concern acut subacut chronic form asf occur differ depend viru virul pig clinic recov infect viraemia persist dai pig viru carrier asf viru isol carrier pig month infect acut form develop high fever clinic sign diseas accompani depress loss appetit rapid difficult breath discharg nose ey pig show uncoordin movement huddl sow abort stage pregnanc pig show vomit constip develop bloodi diarrhoea congest haemorrhag subcutan area evid extrem ear coma develop death occur dai develop clinic sign morbid mortal rate hold post mortem find typic haemorrhag syndrom generalis congest carcas bloodi fluid chest abdomin caviti enlarg dark spleen haemorrhag lymph node resembl blood clot renal gastrohepat lymph node petechi haemorrhag kidnei cortic medullari pyramid renal pelvi abdomin serosa gastric intestin mucosa heart epicardium endocardium hydrothorax petechi haemorrhag pleura gener acut form classic swine fever lead clinic patholog pictur similar african swine fever present haemorrhag skin ear easi detect lead suspicion acut classic african swine fever diseas similar lesion acut african swine fever consid case suspect erysipela porcin reproduct respiratori syndrom coumarin poison purpura haemorragica post wean multisystem wast syndrom porcin dermat nephropathi syndrom salmonella pasteurella infect enter respiratori syndrom fever respond antibiot treatment subacut form subacut form diseas common endem area subacut infect characteris fluctuat fever depress pneumonia death occur due heart failur lesion subacut form similar acut form milder characterist lesion larg haemorrhag lymph node kidnei spleen pulmonari congest oedema case interstiti pneumonia chronic form chronic form diseas rare chronic form secondari bacteri infect observ clinic sign chronic asf specif diseas consid differenti diagnosi increas bodi temperatur necessarili present anim infect hold fever detect pig clinic symptom chronic asf includ respiratori problem abort arthriti chronic skin ulcer necrosi resembl typic clinic pictur asf viru infect lesion minim absent histopatholog find characteris enlarg lymph node spleen pleuriti fibrin pericard infiltr pneumon focal caseou necrosi mineralis lung chapter iii guidelin main criteria consid recognit hold asf suspect hold decis recognis hold suspect hold basi find criteria ground clinic patholog find pig main clinic patholog find consid fever morbid mortal pig ag fever haemorrhag syndrom petechi ecchymot haemorrhag lymph node kidnei spleen enlarg dark acut form urinari bladder ulcer gall bladder epidemiolog find main epidemiolog find consid pig direct indirect contact pig hold proven infect asf viru hold suppli pig subsequ shown infect asf viru sow artifici insemin semen origin suspect sourc indirect direct contact feral pig popul asf occur pig outdoor region feral pig infect asf viru pig fed swill suspicion swill treat inactiv asf viru exposur occur due person enter hold transport come hold suspect infect infect asf viru vector occur area hold case hold consid suspect hold suspicion classic swine fever rais hold due clinic patholog find clinic epidemiolog laboratori investig led confirm diseas identif diseas sourc agent hold question chapter iv check sampl procedur guidelin procedur clinic examin sampl pig suspect hold member state ensur clinic examin sampl laboratori investig carri suspect hold confirm exclud asf accord guidelin procedur laid point irrespect adopt measur refer articl direct ec hold question guidelin procedur appli case diseas asf consid differenti diagnosi includ occas clinic sign epidemiolog pattern diseas observ pig suggest low probabl occurr asf case pig suspect infect asf viru measur refer articl direct ec adopt suspect hold question case suspicion asf pig slaughterhous mean transport guidelin procedur laid point appli mutati mutandi offici veterinarian visit suspect hold confirm rule asf check product health record hold carri record inspect subunit hold carri select pig clinic examin clinic examin includ take bodi temperatur primarili concern pig group pig sick anorex pig pig recent introduc confirm outbreak suspect sourc pig subunit recent visit extern visitor recent close contact asf suspect infect pig riski contact potenti sourc asf viru identifi pig sampl serolog test asf case result test asf rule contact pig pig recent recov diseas inspect suspect hold presenc pig group pig refer subparagraph compet author prejudic measur appli hold question accord direct ec take account epidemiolog situat carri examin hold question accord point ensur blood sampl laboratori test pig hold question case sampl procedur laid point section guidanc purpos adopt maintain measur laid articl direct ec pend investig hold question rule suspicion asf refer made paragraph clinic examin hold question carri pig select random subunit risk introduct asf viru identifi suspect minimum number pig examin detect fever occur preval confid subunit dead moribund pig detect suspect hold post mortem examin carri prefer pig pig shown evid sign diseas death high fever di recent examin shown lesion suggest asf due epidemiolog situat investig deem clinic examin laid point blood sampl laid point carri subunit dead moribund pig post mortem examin carri contact pig pig show clinic sign irrespect presenc absenc lesion suggest asf sampl organ tissu pig subject post mortem examin collect virolog test accord chapter sampl prefer collect recent dead pig post mortem examin carri compet author ensur precaut hygien measur prevent diseas spread case moribund pig kill human accord council direct eec decemb protect anim time slaughter kill amend regul ec clinic sign lesion suggest asf detect suspect hold compet author deem find suffici confirm outbreak asf laboratori test blood sampl laboratori test suspect pig pig subunit suspect pig accord procedur minimum number sampl serolog test detect seropreval confid subunit question number sampl virolog test accord instruct compet author account rang test perform sensit laboratori test epidemiolog situat examin carri suspect hold clinic sign lesion suggest asf detect laboratori test deem compet author rule asf sampl procedur laid point guidanc purpos sampl procedur hold pig kill confirm diseas order manner introduct asf viru infect hold length time elaps introduct establish pig kill hold confirm outbreak accord articl direct ec blood sampl serolog test random pig kill minimum number pig sampl detect seropreval confid pig subunit hold sampl virolog test accord instruct compet author account rang test perform sensit laboratori test epidemiolog situat area presenc vector infect asf viru previous demonstr collect soft tick virolog test accord instruct compet author annex iii direct ec case secondari outbreak compet author decid derog point establish sampl procedur take account epidemiolog inform sourc mean viru introduct hold potenti spread diseas hold sampl procedur pig kill prevent measur suspect hold order asf confirm rule addit epidemiolog inform gain pig kill prevent measur suspect hold accord provis articl direct ec blood sampl serolog test blood sampl virolog test accord procedur laid point sampl primarili concern pig show sign post mortem lesion suggest asf contact pig pig riski contact infect suspect pig suspect contamin asf viru pig sampl accord instruct compet author account epidemiolog situat pig proceed subunit hold sampl random case minimum number sampl serolog test detect seropreval confid subunit question type sampl virolog test test accord instruct compet author account rang test perform sensit test epidemiolog situat check sampl procedur authoris move pig hold locat protect surveil zone case pig slaughter kill articl direct ec prejudic provis articl subparagraph direct ec order authoris move pig hold locat protect surveil zone accord articl direct clinic examin carri offici veterinarian carri hour period move pig accord provis laid case pig move hold addit investig carri accord point clinic examin pig includ take temperatur proport pig carri subunit hold pig move minimum number pig check detect fever occur preval confid subunit case pig move slaughterhous process plant place kill slaughter addit investig carri accord point clinic examin pig carri subunit pig move case pig older month examin includ take temperatur proport pig minimum number pig check detect fever occur preval confid subunit question pig refer point slaughter kill blood sampl serolog test blood organ sampl tonsil spleen lymph node virolog test pig proceed subunit pig move minimum number sampl detect seropreval viru preval confid subunit type sampl test accord instruct compet author account rang test perform sensit test epidemiolog situat clinic sign post mortem lesion suggest asf detect pig slaughter kill derog point provis sampl laid section appli derog provid articl articl direct ec grant compet author ensur intens sampl test scheme appli group pig check sampl refer point context scheme minimum number blood sampl detect seropreval confid group pig question check sampl procedur hold relat repopul pig reintroduc hold accord articl direct ec sampl procedur appli blood sampl collect earliest dai reintroduct pig case sentinel pig reintroduc blood sampl serolog test random number pig detect seropreval confid subunit hold case total repopul blood sampl serolog test random number pig detect seropreval confid subunit hold pig reintroduc hold accord articl direct ec sampl procedur appli blood sampl collect earliest dai reintroduct pig case sentinel pig reintroduc blood sampl serolog test random number pig detect seropreval confid subunit hold case total repopul blood sampl serolog test random number pig detect seropreval confid subunit hold procedur laid indent repeat earliest dai total repopul reintroduct pig compet author ensur case diseas death pig hold due unknown reason pig question immedi test asf provis appli restrict pig movement refer articl direct ec lift hold question sampl procedur hold protect zone lift restrict order measur refer articl direct ec lift protect zone hold zone clinic examin carri accord procedur laid section blood sampl serolog test laid point minimum number blood sampl detect seropreval confid pig subunit hold derog provid articl articl direct ec grant compet author ensur number blood sampl detect seropreval confid subunit hold sampl procedur hold surveil zone lift restrict order measur refer articl direct ec lift surveil zone clinic examin carri hold zone accord procedur laid section addit blood sampl serolog test pig hold sampl deem compet author semen collect centr blood sampl serolog test carri hold locat surveil zone number blood sampl hold accord section sentenc derog provid articl articl direct ec grant compet author ensur hold zone blood sampl serolog test minimum number blood sampl detect seropreval confid subunit hold serolog monitor sampl procedur area asf suspect occur confirm feral pig case serolog monitor feral pig area asf confirm suspect occur size geograph area target popul sampl previous defin order establish number sampl sampl size establish function estim number live anim function number anim shot data popul densiti size geograph area sampl identifi take account continu presenc feral pig presenc natur artifici barrier effici prevent larg continu movement anim circumst occur case larg area recommend identifi sampl area km popul feral pig live prejudic provis articl direct ec minimum number pig sampl defin sampl area detect seropreval confid purpos anim sampl area identifi collect sampl virolog test feral pig shot found dead carri laid chapter virolog monitor shot feral pig deem primarili carri anim year sampl laboratori accompani questionnair refer articl direct ec chapter gener procedur criteria collect transport sampl gener procedur criteria sampl carri suspect hold map hold prepar epidemiolog subunit hold identifi time deem sampl pig pig sampl uniqu mark easili sampl sampl laboratori accompani form accord requir establish compet author form includ detail histori pig sampl clinic sign post mortem lesion observ case pig hold clear inform ag categori hold origin pig sampl provid recommend locat pig sampl hold record uniqu identif mark collect sampl virolog test detect asf viru antigen genom dead human destroi pig tonsil lymph node gastrohepat renal submandibular retropharyng spleen kidnei lung tissu suitabl sampl case autolys carcas entir long bone sternum specimen choic anticoagul blood clot blood sampl collect pig show sign fever sign diseas accord instruct compet author transport sampl recommend sampl properli identifi transport store leak proof contain cool refriger temperatur expect sampl arriv laboratori hour laboratori contact obtain instruct temperatur condit transport deliv laboratori quickli packag ic pack dry ic cool tissu organ separ seal plastic contain properli label larger contain pack suffici absorb materi protect damag absorb leakag directli transport laboratori compet person order rapid reliabl transport ensur packag label address recipi laboratori messag promin displai quot anim patholog materi perish fragil open asf laboratori quot respons person laboratori receiv sampl inform due time arriv sampl air transport sampl commun refer laboratori asf packag label iata regul chapter vi principl virolog test evalu result detect viru antigen direct immuno fluoresc test dift principl test microscop detect viral antigen impress smear thin cryosect organ materi pig suspect infect asf viru intracellular antigen detect fit conjug specif antibodi fluoresc inclus bodi granul cytoplasm infect cell suitabl organ kidnei spleen lymph node smear bone marrow cell feral pig organ autolys test perform hour organ sampl obtain dead anim screen purpos limit highli sensit test case acut asf subacut chronic form dift present sensit approxim due presenc antigen antibodi complex block reaction asf conjug antibodi confid test result limit doubt stain consider experi perform test acquir organ test autolys elisa antigen detect viral antigen detect elisa techniqu recommend acut form diseas low sensit antigen antibodi complex present sensit antigen elisa high score posit result anim show clinic sign acut asf case recommend test quot herd quot test conjunct virolog test viru isol identif haemadsorpt test viru isol base inocul sampl materi suscept primari cell cultur porcin origin monocyt macrophag cell prefer sampl isol asf viru blood leucocyt obtain coagul blood sampl organ refer section asf viru present sampl replic cell characterist cytopath effect produc infect cell techniqu recommend identif asf viru isol due high sensit specif base capabl asf viru replic pig macrophag induc haemadsorpt presenc pig erythrocyt characterist quot rosett quot erythrocyt develop infect macrophag small number field asf viru strain induc haemadsorpt produc cytopath effect strain specif identifi dif test sediment cell cultur pcr viru isol suit investig sampl small number anim mass surveil viru isol procedur labour intens requir dai result cell cultur passag order detect small amount viru sampl lead investig time dai final result obtain autolys sampl cytotox cell cultur limit viru isol identif recommend refer test confirm posit result prior elisa pcr dift recommend asf confirm method case primari outbreak case asf asf virus isol pig macrophag viru characteris molecular epidemiolog asf viru isol primari outbreak primari case feral pig case slaughterhous mean transport characteris nation refer laboratori member state laboratori authoris member state question commun refer laboratori accord section case viru isol commun refer laboratori viru collect delai detect viru genom polymeras chain reaction pcr appli detect viru genom blood serum tissu organ sampl small fragment viral dna amplifi pcr detect quantiti wide rang isol belong viru genotyp includ haemadsorb virus isol low virul detect primer highli conserv region genom test detect genom sequenc viru pcr posit infecti viru detect viru isol autolys tissu sampl convalesc pig pig recov clinic normal pcr limit number sampl carefulli select suspect anim recommend method organ sampl cytotox viru isol sampl feral pig suitabl sampl materi pcr organ viru isol serum tick homogen analys pcr pcr perform work dai requir laboratori equip separ facil skill staff advantag infecti viru replic laboratori pcr highli sensit contamin easili occur lead fals posit result stringent qualiti control procedur essenti recommend virolog test evalu result virolog test essenti confirm asf viru isol consid refer virolog test confirmatori test recommend posit dif pcr result detect clinic sign lesion diseas doubt case primari outbreak asf confirm clinic sign lesion diseas detect pig question distinct antigen genom antibodi detect test posit result sampl suspect pig secondari outbreak asf confirm addit epidemiolog link confirm outbreak case clinic sign lesion diseas detect pig question antigen genom antibodi detect test posit result primari case asf feral pig confirm viru isol antigen genom antibodi detect test posit result case asf feral pig epidemiolog link previous confirm case found confirm antigen genom antibodi detect test posit result genet characteris asf viru isol genet characteris asf viru isol achiev determin restrict enzym pattern nucleotid sequenc portion viru genom similar restrict pattern sequenc obtain previou viru isol outbreak diseas caus virus follow european african molecular model genet characteris asf viru isol major import improv current knowledg molecular epidemiolog asf genet variat virus molecular data isol classifi provid inform origin viru molecular characteris perform nation laboratori laboratori authoris diagnos asf short delai origin sampl viru isol commun refer laboratori molecular characteris data restrict enzym analysi sequenc asf viru isol laboratori authoris diagnos asf forward commun refer laboratori inform put databas laboratori inform includ databas nation refer laboratori member state purpos public scientif journal request laboratori question commun refer laboratori guarante confidenti data publish chapter vii principl serolog test evalu result basic principl diagnost asf specif antibodi detect recommend subacut chronic form larg scale test asf erad programm reason antibodi rapidli produc infect pig pig antibodi detect serum sampl ten dai infect ii vaccin asf mean asf specif antibodi induc asf viru infect iii long last antibodi respons pig recov diseas specif antibodi detect high level month lifetim pig specif asf antibodi matern origin detect piglet week life half life matern antibodi piglet week found piglet older month asf antibodi matern origin detect antibodi asf viru serum plasma exud organ submit carri assist diagnosi asf suspect hold estim date introduct infect case confirm outbreak monitor surveil purpos locat seroposit pig hold provid valuabl inform asf viru enter hold accur evalu result serolog test carri take account clinic virolog epidemiolog find framework enquiri carri case suspicion confirm asf accord articl direct ec recommend serolog test elisa indirect immunofluoresc test iift immunoblot ib test test choic serolog confirm asf qualiti effici serolog diagnosi perform nation laboratori regularli check framework inter laboratori comparison test period organis commun refer laboratori elisa test reliabl test larg scale serolog studi base detect asf viru antibodi bound viral protein attach solid phase addit protein conjug enzym produc visibl colour reaction react substrat qualiti control sensit specif batch elisa reagent regularli perform nation laboratori make panel refer sera provid commun refer laboratori panel includ sera pig earli phase asf viru infect dai post infect sera convalesc pig dai post infect elisa serolog diagnosi asf detect refer sera convalesc pig result obtain refer sera reproduc recommend posit sera earli phase detect result obtain refer sera pig earli phase infect give indic sensit elisa iift rapid techniqu high sensit specif detect asf antibodi sera tissu exud base detect asf antibodi bind monolay ms cell infect adapt asf viru antibodi antigen reaction detect label fluorescein protein posit sampl show specif fluoresc nucleu infect cell dift iift combin test organ blood exud collect anim show clinic sign asf lead rapid reliabl confirm diseas ib test highli specif sensit techniqu base nitrocellulos strip viral protein antigen specif antibodi antigen reaction detect addit protein peroxidas conjug substrat test sera inconclus elisa test chapter viii minimum safeti requir asf laboratori requir laid tabl fulfil laboratori asf viru amplifi replic cell cultur post mortem examin process tissu dift pcr serolog inactiv antigen carri lower contain level provid minimum requir tabl fulfil basic hygien post oper disinfect safe dispos carcas tissu sera carri requir laid tabl fulfil laboratori anim inocul asf viru stock asf viru secur storag frozen freez dri individu ampoul label comprehens record maintain viru stock date result qualiti control check record virus ad stock detail sourc virus issu laboratori recommend biosecur unit asf viru work support area asf viru manipul area prepar glasswar media mainten prepar infect cell cultur process sera serolog test method live asf viru provis administr cleric support tabl principl biolog contain diagnost laboratori gt tabl gt tabl bio safeti requir experiment anim room gt tabl gt decid number sampl laboratori test sensit test consid number anim sampl higher manual sensit test high defin articl direct ec oj oj derog provid articl direct ec appli sampl concern subunit hold pig kill prejudic examin sampl carri remain pig hold carri accord instruct compet author compet author limit applic prevent kill part hold pig suspect infect contamin asf viru accord articl direct ec sampl concern subunit hold measur appli prejudic examin sampl carri remain pig hold carri accord instruct compet author recommend collect sampl ileum diagnosi classic swine fever commun refer laboratori open licenc receiv diagnost sampl asf viru isol member state sampl proce eu copi import permit request laboratori transport attach envelop packag fluorescein isothiocyan