commiss direct approxim law member state relat method analysi check composit cosmet product eec commiss european commun regard treati establish european econom commun regard council direct eec juli approxim law member state relat cosmet product amend direct eec articl thereof direct eec offici test cosmet product aim ensur condit laid commun provis composit cosmet product satisfi method analysi drawn quickli step attain object definit method commiss direct eec step consist definit method identif oxid agent determin hydrogen peroxid cosmet hair care product identif semi quantit determin oxid color hair dye identif determin nitrit identif determin free formaldehyd determin resorcinol shampoo hair lotion determin methanol relat ethanol propan ol measur laid direct accord opinion committe adapt direct eec technic progress adopt direct articl member state step ensur offici test cosmet product identif oxid agent determin hydrogen peroxid hair care product identif semi quantit determin oxid color hair dye identif determin nitrit identif determin free formaldehyd oj oj oj determin resorcinol shampoo hair lotion determin methanol relat ethanol propan ol perform accord method annex articl member state bring forc law regul administr provis compli direct decemb forthwith inform commiss thereof articl direct address member state brussel commiss karl heinz narj member commiss annex identif oxid agent determin hydrogen peroxid hair care product purpos scope iodometr determin hydrogen peroxid cosmet absenc oxid agent form iodin iodid iodometr determin hydrogen peroxid detect identifi oxid agent present identif break stage cover persulph bromat hydrogen peroxid cover barium peroxid identif persulph bromat hydrogen peroxid principl sodium persulph potassium persulph ammonium persulph potassium bromat sodium bromat hydrogen peroxid origin barium peroxid identifi mean descend paper chromatographi made develop solvent reagent reagent analyt puriti aqueou refer solut compound sodium persulph potassium persulph ammonium persulph potassium bromat sodium bromat hydrogen peroxid develop solvent ethanol develop solvent benzen methanol methyl butan ol water vol detect agent aqueou solut potassium iodid detect agent aqueou solut starch detect agent hydrochlor acid hydrochlor acid apparatu equip chromatographi paper whatman paper equival micropipett standard flask ml flute filter apparatu descend paper chromatographi sampl prepar water solubl product make solut sampl dissolv product ml water solut carri paper chromatographi section product sparingli solubl water weigh sampl dispers ml water make ml water case mix filter dispers flute filter filtrat order carri paper chromatographi section prepar dispers sampl dispers ml water acidifi dilut hydrochlor acid make water ml mix filter dispers flute filter filtrat order carri paper chromatographi section cream dispers product ml water dispers carri paper chromatographi section method put quantiti solvent separ chromatographi tank order carri descend paper chromatographi satur chromatographi tank hour solvent vapour appli sampl solut refer solut prepar section start point strip chromatographi paper whatman equival cm long cm wide suitabl format evapor solut air place chromatographi strip chromatographi tank fill develop solvent develop solvent front advanc cm hour repeat procedur section chromatographi paper whatman equival develop solvent chromatograph solvent front advanc cm hour develop remov chromatogram dry air reveal spot chromatogram sprai success detect agent shortli detect agent spot persulph chromatogram hydrogen peroxid spot mark spot pencil detect agent chromatogram obtain accord section presenc bromat greyish blue spot chromatogram abovement condit pertain develop solvent rf valu refer substanc approxim gt pic file quot quot gt identif barium peroxid principl barium peroxid identifi format hydrogen peroxid acidif sampl presenc barium ion absenc persulph ad dilut sulphur acid portion acid sampl solut result white precipit barium sulphat form presenc barium ion sampl confirm paper chromatographi manner barium peroxid persulph present simultan digest residu solut alkali dissolut hydrochlor acid presenc barium ion confirm solut melt paper chromatographi barium sulphat precipit reagent methanol concentr hydrochlor acid hydrochlor acid sulphur acid rhodizon acid disodium salt barium chlorid bacl anhydr sodium carbon aqueou solut barium chlorid develop solvent consist methanol concentr hydrochlor acid concentr water vol detect agent aqueou solut rhodizon acid disodium salt freshli prepar immedi apparatu equip micropipett platinum crucibl standard flask ml chromatographi paper schleicher schull equival clean paper develop overnight descend chromatographi tank develop solvent dry flute filter paper usual apparatu carri ascend paper chromatographi sampl prepar product persulph absent dispers product ml water bring ph dispers hydrochlor acid transfer dispers water ml standard flask make mark water mix dispers paper chromatographi analysi section identif barium precipit sulphat product persulph present dispers product ml water filter add dri residu time weight sodium carbon mix melt mixtur platinum crucibl half hour cool room temperatur dissolv melt ml water filter dissolv residu melt hydrochlor acid make ml water solut paper chromatographi analysi section identif barium precipit sulphat method place quantiti develop solvent tank ascend paper chromatographi satur tank hour piec chromatographi paper pretreat section appli solut prepar accord section refer solut start point dry sampl refer spot air develop chromatogram solvent front ascend cm remov chromatogram tank dry air reveal spot chromatogram sprai paper detect agent presenc barium red spot rf chromatogram determin hydrogen peroxid principl iodometr determin hydrogen peroxid base reaction gt pic file quot quot gt convers proce slowli acceler addit ammonium molybd iodin form determin titrimetr sodium thiosulph measur hydrogen peroxid content definit hydrogen peroxid content measur manner express percentag mass product reagent reagent analyt puriti sulphur acid potassium iodid ammonium molybd sodium thiosulph potassium iodid solut freshli prepar immedi ammonium molybd solut starch solut apparatu equip beaker ml burett ml standard flask ml measur cylind ml mark pipett ml conic flask ml method ml beaker weigh gram product hydrogen peroxid transfer content water ml standard flask make mark water mix pipett ml sampl solut ml conic flask add success ml sulphur acid ml potassium iodid solut drop ammonium molybd solut titrat iodin form immedi sodium thiosulph solut end point reach add millilitr starch solut indic record consumpt sodium thiosulph millilitr manner section carri blank determin replac ml sampl solut ml water record consumpt sodium thiosulph blank determin vo ml calcul calcul hydrogen peroxid content product percentag mass aid formula gt pic file quot quot gt repeat product hydrogen peroxid differ result determin parallel carri sampl exce absolut norm iso ii identif semi quanitt determin oxid color hair dye purpos scope method suitabl identif semi quantit determin substanc hair dye cream liquid form gt pic file quot quot gt principl oxid color extract ph ethanol dye cream liquid form identifi thin layer chromatographi dimension semi quantit determin substanc chromatogram sampl compar mean develop system refer substanc produc time similar condit reagent reagent analyt puriti ethanol anhydr aceton ethanol ammonia solut ascorb acid chloroform cyclohexan nitrogen technic grade toluen benzen butanol butan ol hypophosphor acid solut diazo reagent nitro benzenediazonium chlorobenzenesulphon stabil salt form red jn francolor chloro nitro benzenediazonium naphthalenebenzo stabil salt form nncd reagent refer fluka equival silver nitrat dimethylaminobenzaldehyd dimethylphenol ferric chlorid hexahydr hydrochlor acid solut refer substanc refer substanc shown paragraph quot purpos scope quot case amin compound refer substanc hydrochlorid mono di free base refer solut solut refer substanc section prepar weigh mg mg refer substanc standard ml flask add ml ethanol mg ascorb acid make solut alkalin addit ammonia solut give appar ph test indic paper make ml ethanol mix solut week cool place light case addit ascorb acid ammonia precipit form allow settl proceed develop solvent aceton chloroform toluen vol chloroform cyclohexan absolut ethanol ammonia vol benzen butan ol water vol shake upper phase separ room temperatur butanol chloroform reagent vol separ carefulli room temperatur lower phase gt pic file quot quot gt note develop solvent ammonia shaken immedi indic sprai diazo reagent make aqueou solut chosen reagent solut freshli prepar ehrlich reagent dissolv diamethylaminobenzaldehyd ml hydrochlor acid aqueou solut dimethylphenol ferric chlorid hexahydr solut dissolv dimethylphenol ml ethanol solut dissolv ferric chlorid hexahydr ml ethanol develop solut sprai separ solut solut ammoniac silver nitrat ammonia ad aq solut silver nitrat precipit dissolv reagent prepar immedi apparatu usual laboratori equip thin layer chromatographi plastic glass cover construct chromatograph plate surround nitrogen applic spot dry precaut suscept oxid color micro syring graduat divis squar cut needl repeat dispens mount clamp stand plate nitrogen silica gel thin layer plate readi mm thick form cm macherei nagel silica hr plastic support equival centrifug rev min centrifug tube ml ptfe line screw cap equival procedur treatment test sampl discard cm cream extrud tube put centrifug tube previous flush nitrogen mg ascorb acid cream homogen liquid add ammonia drop wise ph make ml ethanol homogen nitrogen stopper centrifug rev min minut supernat liquid chromatographi spot plate atmospher nitrogen appli chromatographi plate refer solut point situat cm line approxim cm edg plate refer solut spot arrang gt pic file quot quot gt addit appli point test solut sampl obtain section plate nitrogen moment chromatograph develop place plate tank previous flush nitrogen satur solvent develop room temperatur dark solvent front move cm baselin remov plate dry nitrogen room temperatur sprai sprai plate immedi solut identif compar rf colour obtain sampl refer substanc chromatograph tabl exampl rf valu colour substanc depend solvent indic confirm doubt identif achiev spike method ad refer substanc solut sampl extract semi quantit estim compar visual intens spot substanc identifi rang concentr refer substanc concentr substanc found sampl excess dilut sampl extract repeat measur gt pic file quot quot gt examin dimension thin layer chromatographi dimension chromatograph procedur requir addit standard reagent addit refer solut substanc gt pic file quot quot gt aminophenol oap aminophenol map aminophenol pap nitro phenylenediamin nppd nitro phenylenediamin nopd prepar solut addit refer substanc addit develop solvent ethyl acet cyclohexan ammonia solut vol addit indic system place glass vessel develop tank thin layer chromatographi add crystal iodin close tank adequ lid chromatographi draw line shown figur absorb surfac thin layer plate nitrogen atmospher appli extract base point figur cm side quantiti extract depend intens spot chromatogram divid point figur oxid color identifi assum identifi distanc point cm appli refer solut dap appli conduct oper nitrogen repeat oper base point figur plate nitrogen moment chromatograph distanc point cm flush chromatograph tank nitrogen place suitabl quantiti develop solvent place plate tank develop elut direct figur dark elut solvent front reach line mark plate approxim cm remov plate tank place chromatographi tank previous flush nitrogen evapor elut solvent minut graduat test tube place suitabl quantiti elut solvent tank flush nitrogen place plate rotat tank chromatograph direct dark solvent front reach line drawn absorb surfac remov plate tank evapor elut solvent air place plate minut chromatographi tank iodin vapour interpret chromatogram rf colour valu refer substanc chromatograph time tabl ii guid rf valu colour note obtain maximum colour spot leav chromatogram expos atmospher half hour develop presenc oxid color found definit confirm repeat oper ad base point top amount extract refer substanc identifi spot found comparison chromatogram obtain interpret chromatogram correct gt pic file quot quot gt iii identif determin nitrit identif purpos scope method suitabl identif nitrit cosmet product cream past principl presenc nitrit format colour deriv gt pic file quot quot gt reagent reagent analyt puriti dilut sulphur acid dilut ml concentr sulphur acid ml distil water dilut hydrochlor acid dilut ml concentr hydrochlor acid ml distil water methanol gt pic file quot quot gt add drop wise ml dilut hydrochlor acid shake fill mark methanol mix solut complet clear store solut brown glass bottl apparatu beaker ml standard flask ml brown glass bottl ml glass plate cm plastic spatula filter paper cm procedur spread part sampl examin evenli glass plate cover surfac thick cm soak sheet filter paper distil water lai sampl press filter paper plastic spatula wait minut appli centr filter paper drop dilut sulphur acid gt pic file quot quot gt second remov filter paper examin daylight presenc nitrit reddish purpl color nitrit content low reddish purpl colour yellow second chang colour take place minut larg quantiti nitrit present comment intens reddish purpl colour time elaps chang yellow give indic nitrit content sampl determin purpos method describ determin nitrit cosmet product definit nitrit content sampl determin method express mass sodium nitrit principl dilut sampl water clarifi nitrit present made react sulphanilamid naphthylethylenediamin optic densiti colour obtain measur nm reagent reagent analyt qualiti clarif reagent reagent week prepar carrez reagent dissolv potassium cyanoferr ii fe cn distil water dilut water ml carrez ii reagent dissolv zinc acet zn ch coo ml glacial acet acid distil water dilut water ml sodium nitrit solut dissolv sodium nitrit distil water ml volumetr flask dilut water mark dilut ml stock standard solut ml ml solut microgram nano sodium hydroxid solut sulphanilamid hydrochlorid solut dissolv sulphanilamid ml water warm cool add ml concentr hydrochlor acid stir dilut water ml hydrochlor acid naphthyl reagent solut prepar dai dissolv naphthylethylenediamin dihydrochlorid water dilut water ml apparatu analyt balanc volumetr flask ml bulb graduat pipett measur cylind ml flute filter paper free nitrit diamet cm water bath spectrophotomet optic cell cm path length ph meter microburett ml beaker ml procedur weigh gram precis mg homogen sampl transfer hot distil water quantit ml beaker bring volum approxim ml hot distil water place beaker water bath half hour period shake content occasion cool room temperatur add success stir ml carrez reagent ml carrez ii reagent add sodium hydroxid solut bring ph ph meter transfer content quantit ml volumetr flask make mark distil water mix content filter flute filter paper pipett suitabl aliquot ml clear filtrat ml ml volumetr flask add distil water volum ml mix add ml sulphanilamid hydrochlorid solut ml hydrochlor acid mix stand minut add ml naphthyl reagent mix stand minut dilut water mark mix prepar blank test repeat oper addit naphthyl reagent measur optic densiti nm solut obtain blank solut refer read calibr graph sodium nitrit content microgram ml solut microgram correspond optic densiti measur ml sodium nitrit solut prepar calibr graph concentr sodium nitrit ml calcul calcul sodium nitrit content sampl percent mass aid formula gt pic file quot quot gt repeat content sodium nitrit differ result determin parallel carri sampl exce absolut iv identif determin free formaldehyd purpos scope method describ identif determin free formaldehyd applic cosmet product compris part identif determin pentan dion colorimetri method inadequ formaldehyd combin polymer case formaldehyd donor result exce maximum permit concentr method determin bisulphit method formaldehyd combin polymer compound account unstabl combin hexamethylen tetramin determin alkalin measur difficult presenc buffer solut definit free formaldehyd content sampl determin method express percentag mass principl part identif formaldehyd sulphur acid medium turn schiff reagent pink mauv part ii determin pentan dion formaldehyd react pentan dion presenc ammonium acet form diacetyl dihydrolutidin extract butan ol absorb extract measur nm norm iso part iii determin bisulphit formaldehyd react sulphit acid medium form addit compound excess proton titrat sodium hydroxid proton consum form basi calcul determin quantiti formaldehyd blank test sulphit enabl alkalin acid medium measur reagent reagent analyt puriti glacial acet acid anhydr ammonium acet butan ol sulphur acid freshli prepar sodium sulphit solut schiff reagent mg fuchsin weigh beaker dissolv ml water cool add sodium sulphit heptahydr na ml concentr hydrochlor acid make ml reagent unsuit week pentan dion reagent ml volumetr flask dissolv gt pic file quot quot gt make ml water ph solut reagent freshli prepar standard sulphur acid solut standard sodium hydroxid solut iodin solut sodium thiosulph formaldehyd stock solut pour formaldehyd solut ml volumetr flask make ml determin strength solut remov ml add ml standard iodin solut ml sodium hydroxid solut stand minut add ml hcl titrat excess standard iodin solut standard sodium thiosulph solut starch solut indic ml iodin solut equival mg formaldehyd formaldehyd refer solut pipett ml stock solut ml graduat flask make ml deminer water pipett ml solut ml graduat flask make ml deminer water ml solut formaldehyd calcul exact content thymolphthalein solut ml ethanol refer reagent solut reagent pentan dion apparatu standard laboratori apparatu phase separ filter whatman ps equival centrifug spectrophomet glass cell optic path cm potentiomet chart record glass calomel electrod recommend special low temperatur electrod procedur identif weigh test sampl ml beaker add drop sulphur acid ml schiff reagent reagent absolut colourless shake leav stand minut pink mauv tint observ minut formaldehyd present excess determin procedur procedur determin pentan dion colorimetri sampl solut ml volumetr flask weigh quantiti gram test sampl presum quantiti formaldehyd microgram make ml deminer water mix ml erlenmey flask add ml solut ml pentan dion reagent deminer water final volum ml refer solut interfer due background colour test sampl elimin refer solut ml erlenmey flask add ml solut ml refer reagent solut deminer water final volum ml blank solut ml erlenmey flask add ml pentan dion reagent deminer water final volum ml determin shake flask immers water bath minut cool minut bath ic water transfer ml separ funnel ml butan ol rins flask ml water add rins funnel shake mixtur vigor second separ filter measur cell phase separ filter centrifug rev min minut practic take longer measur optic densiti nm extract sampl solut extract refer solut similarli measur extract blank solut butan ol note oper carri minut moment erlenmey flask water bath calibr curv ml erlenmey flask place ml standard solut ml pentan dion reagent deminer water final volum ml continu section measur optic densiti butan ol repeat procedur ml standard solut obtain proce construct calibr curv subtract optic densiti obtain beer law valid formaldenyd determin bisulphit prepar test sampl test tare beaker weigh nearest quantiti test sampl gram presum quantiti mg formaldehyd refer test similar manner weigh refer test sampl gram determin place ml sodium sulphit ml beaker add ml sulphur acid shake immers beaker mixtur ic salt purpos maintain temperatur pour test sampl titrat rapidli potentiometri sodium hydroxid shake continu maintain temperatur neutral point li ph volum sodium hydroxid blank test nitrat addition prepar solut condit volum sodium hydroxid refer test determin acid alkalin sampl potentiometr titrat sodium hydroxid sulphur acid test sampl volum sodium hydroxid sulphur acid note strict observ test condit import perform determin presenc thymolphthalein indic present result calcul colorimetr method subtract read calibr curv amount microgram formaldehyd test solut calcul formaldehyd content sampl percentag mass aid formula gt pic file quot quot gt calcul bisulphit titrat method relat volum sodium hydroxid sulphur acid refer test mass gt pic file quot quot gt neutral product case acid product gt pic file quot quot gt case alkalin product gt pic file quot quot gt remark result method differ lower figur repeat formaldehyd content differ result determin parallel carri sampl exce colorimetr method bisulphit method norm iso determin resorcinol shampoo hair lotion purpos scope method specifi ga chromatograph determin resorcinol shampoo hair lotion method suitabl concentr mass sampl definit content resorcinol sampl determin method express percentag mass principl resorcinol dihydroxytoluen methylresorcinol ad intern standard separ sampl thin layer chromatographi compound isol scrape spot thin layer plate extract methanol final extract compound dri silyl determin ga chromatographi reagent reagent analyt grade hydrochlor acid methanol ethanol readi made silica gel tlc sheet plastic aluminium fluoresc indic deactiv sprai ordinari pre coat silica sheet water glaze sprai plate dry air room temperatur hour note plate deactiv loss resorcinol occur irrevers adsorpt silica develop solvent aceton chloroform acet acid volum resorcinol standard solut dissolv mg resorcinol ml ethanol ml correspond resorcinol intern standard solut dissolv mg dihydroxytoluen dht ml ethanol ml correspond dht standard mixtur mix ml solut ml solut ml volumetr flask make mark ethanol mix ml correspond resorcinol dht silyl agent bi trimethylsilyl trifluoroacetamid bstfa hexamethyldisilazan hmd trimethylchlorosilan tmc apparatu usual thin layer ga chromatographi equip glasswar procedur prepar sampl weigh accur ml beaker test portion gram product approxim mg resorcinol acidifi hydrochlor acid mixtur acid approxim ml need add ml mg dht intern standard solut mix transfer ml volumetr flask ethanol make mark ethanol mix appli solut deactiv silica sheet continu line approxim cm length care line narrow appli standard mixtur plate spot point start line solut aid local plate develop develop plate unlin unsatur tank fill develop solvent solvent front reach line cm start line take minut air dry plate local resorcinol dht zone short wave uv light nm compound approxim rf valu mark band pencil mm distanc dark borderlin band remov zone collect adsorb band ml bottl extract adsorb sampl absorb standard mixtur add ml methanol extract hour continu stir filter mixtur repeat extract minut ml methanol combin extract evapor solvent dry overnight vacuum desicc fill suitabl desicc appli heat silyl residu add bstfa microsyring leav mixtur close vessel hour room temperatur add success hmd tmc microsyring heat mixtur minut close vessel cool mixtur ga chromatographi chromatograph condit column yield resolut equal gt pic file quot quot gt column ga chromatograph condit found suitabl gt pic file quot quot gt set hydrogen air flow follow manufactur instruct inject solut obtain ga chromatograph carri inject solut measur peak area averag calcul peak area ratio peak area resorcinol peak area dht calcul concentr resorcinol sampl express mass gt pic file quot quot gt repeat resorcinol content differ result determin parallel carri sampl exce absolut vi determin methanol relat ethanol propan ol purpos scope method describ ga chromatograph analysi methanol kind cosmet product includ aerosol rel level determin definit methanol content determin method express mass methanol relat ethanol propan ol principl determin carri ga chromatographi norm iso reagent analyt grade reagent methanol ethanol absolut propan ol chloroform freed alcohol wash water apparatu ga chromatograph katharomet detector aerosol sampl flame ioniz detector aerosol sampl volumetr flask ml pipett ml ml ml microsyring aerosol sampl special ga tight syring slide valv sampl procedur figur procedur sampl prepar aerosol product sampl chapter ii annex commiss direct eec decemb analyz ga chromatographi condit aerosol product sampl abovement chapter ii dilut water level ethanol propan ol analyz ga chromatographi condit ga chromatographi aerosol sampl katharomet detector column fill hallcomid chromosorb waw mesh column yield resolut equal gt pic file quot quot gt condit resolut achiev gt pic file quot quot gt oj gt pic file quot quot gt aerosol sampl column fill chromosorb porapak qs flame ioniz detector column yield resolut equal gt pic file quot quot gt resolut achiev condit gt pic file quot quot gt standard graph ga chromatographi procedur hallcomid column standard mixtur prepar mixtur measur pipett find exact amount weigh pipett flask addit gt pic file quot quot gt inject chromatograph condit calcul peak area ratio methanol ethanol methanol propan ol mixtur plot standard graph axi methanol relat ethanol propan ol axi peak area ratio methanol ethanol methanol propan ol ga chromatographi procedur porapak qs chromosorb standard mixtur prepar mixtur measur microsyring pipett find exact amount weigh pipett flask addit gt pic file quot quot gt inject chromatograph condit calcul peak area ratio methanol ethanol methanol propan ol mixtur plot standard graph axi methanol relat ethanol propan ol axi peak area ratio methanol ethanol methanol propan ol standard graph straight line repeat methanol content rel ethanol propan ol differ result determin parallel carri sampl exce norm iso