commiss direct eec septemb method analysi check composit cosmet product commiss european commun regard treati establish european econom commun regard council direct eec juli approxim law member state relat cosmet product amend direct eec articl thereof direct eec offici test cosmet product aim ensur condit laid commun provis composit cosmet product satisfi method analysi laid quickli step commiss direct eec amend direct eec commiss direct eec amend direct eec commiss direct eec eec identif determin silver nitrat identif determin silver nitrat identif determin selenium disulphid anti dandruff shampoo determin solubl barium solubl strontium pigment form salt lake identif determin benzyl alcohol identif zirconium determin zirconium aluminium chlorin aerosol antiperspir identif determin hexamidin dibromohexamidin dibromopropamidin chlorhexidin constitut step measur provid direct accord opinion committe adapt direct eec technic progress adopt direct articl member state step ensur offici test cosmet product identif determin silver nitrat identif determin selenium disulphid anti dandruff shampoo determin solubl barium solubl strontium pigment form salt lake identif determin benzyl alcohol identif zirconium determin zirconium aluminium chlorin aerosol antiperspir identif determin hexamidin dibromohexamidin dibromopropamidin chlorhexidin carri accord method annex articl member state bring forc law regul administr provis need compli direct septemb forthwith inform commiss thereof member state adopt provis refer direct accompani refer time offici public procedur refer adopt member state member state commun commiss provis nation law adopt field cover direct articl direct address member state brussel septemb commiss christian scriven member commiss oj oj oj oj oj oj oj oj annex identif determin silver nitrat cosmet product identif scope field applic method describ identif silver nitrat silver aqueou cosmet product principl silver identifi characterist white precipit form chlorid ion reagent reagent analyt puriti hydrochlor acid solut ammonia solut dilut concentr ammonium hydroxid solut ml equal quantiti water mix nitric acid solut apparatu normal laboratori equip centrifug procedur sampl centrifug tube add hydrochlor acid solut dropwis precipit complet mix centrifug discard supernat liquid wash precipit drop cold water reject wash add quantiti water equal bulk precipit centrifug tube heat boil stir centrifug hot discard supernat liquid precipit add drop ammonia solut mix centrifug drop supernat liquid glass slide add drop nitric acid solut white precipit presenc silver determin scope field applic method suitabl determin silver nitrat silver cosmet product intend dye eyelash eyebrow principl silver determin product atom absorpt spectrometri reagent reagent analyt puriti nitric acid solut silver standard solut stock silver standard solut mg ml nitric acid solut spectrosol equival silver standard solut mg ml transfer pipett ml stock silver standard solut ml volumetr flask make volum nitric acid solut mix standard solut freshli prepar store dark colour glass bottl apparatu normal laboratori equip atom absorpt spectrophotomet equip silver hollow cathod lamp procedur sampl prepar weigh accur approxim gram homogen sampl product transfer quantit litr volumetr flask make volum nitric acid solut mix condit atom absorpt spectrometri flame air acetylen wavelength nm background correct fuel condit lean maximum absorb optim burner height fuel condit calibr seri ml volumetr flask transfer pipett ml silver standard solut make flask volum nitric acid solut mix solut mg silver millilitr measur absorb nitric acid solut obtain silver concentr calibr curv measur absorb silver calibr standard plot calibr curv relat absorb valu silver concentr determin measur absorb sampl solut calibr curv read concentr silver absorb obtain sampl solut calcul calcul silver nitrat content sampl percentag mass formula silver nitrat mass gram sampl analysi concentr silver sampl solut microgram millilitr obtain calibr curv repeat silver nitrat content differ result determin carri parallel sampl exce identif determin selenium disulphid anti dandruff shampoo identif scope field applic method describ identif selenium disulphid selenium anti dandruff shampoo principl selenium identifi characterist yellow orang colour produc reaction urea potassium iodid reagent reagent analyt puriti nitric acid concentr ml urea potassium iodid solut dissolv potassium iodid ml water apparatu normal laboratori equip digest tube ml capac heat block digestor filter paper whatman equival mm membran filter procedur approxim shampoo digest tube add ml concentr nitric acid digest minut heat block digestor dilut digest sampl ml water filter filter paper mm membran filter ml filtrat add ml water urea boil cool add ml potassium iodid solut yellow orang colour darken rapidli stand presenc selenium determin scope field applic method suitabl determin selenium disulphid selenium anti dandruff shampoo selenium disulphid principl sampl digest nitric acid selenium result digest determin mean atom absorpt spectrometri reagent reagent analyt puriti nitric acid concentr ml nitric acid solut add ml concentr nitric acid ml water beaker stir continu transfer solut litr volumetr flask make volum water stock selenium standard solut mg ml nitric acid solut spectrosol equival apparatu normal laboratori equip digest tube ml capac heat block digestor filter paper whatman equival mm membran filter atom absorpt spectrophotomet equip selenium hollow cathod lamp procedur sampl prepar weigh accur approxim gram homogen sampl shampoo digest tube add ml concentr nitric acid digest hour heat block digestor solut cool dilut ml water filter filter paper mm membran filter retain filter solut determin condit atom absorpt spectrometri flame air acetylen wavelength nm background correct fuel condit lean maximum absorb optim burner height fuel condit calibr seri ml volumetr flask transfer pipett ml stock selenium standard solut make flask volum nitric acid solut mix solut mg selenium millilitr measur absorb nitric acid solut obtain selenium concentr calibr curv measur absorb selenium calibr standard plot calibr curv relat absorb valu selenium concentr determin measur absorb sampl solut calibr curv read concentr selenium absorb obtain sampl solut calcul calcul selenium disulphid content sampl percentag mass formula selenium disulphid mass gram sampl analysi concentr selenium sampl solut microgram millilitr obtain calibr curv repeat selenium disulphid content differ result determin carri parallel sampl exce determin solubl barium strontium pigment form salt lake determin solubl barium scope field applic method describ procedur extract determin solubl barium pigment form salt lake principl pigment extract hydrochlor acid solut defin condit amount barium extract determin atom absorpt spectrometri reagent reagent analyt puriti ethanol absolut hydrochlor acid solut hydrochlor acid solut potassium chlorid solut dissolv potassium chlorid ml hydrochlor acid solut barium standard solut stock barium standard solut mg ml nitric acid solut spectrosol equival barium standard solut mg ml transfer pipett ml stock barium standard solut ml volumetr flask make volum hydrochlor acid solut mix apparatu normal laboratori equip ph meter accuraci unit wrist action flask shaker membran filter pore size mm atom absorpt spectrophotomet equip barium hollow cathod lamp procedur sampl prepar weigh accur approxim gram pigment conic flask ensur suffici volum effect agit flask capac ml add pipett ml ethanol rotat flask ensur wet pigment add burett exact quantiti hydrochlor acid solut requir give volum acid mass pigment ratio millilitr gram total volum extract includ ethanol ml swirl content flask second ensur mix content ph meter measur ph result suspens add hydrochlor acid solut dropwis rang stopper immedi shake flask minut wrist action flask shaker shaker oper suffici high speed produc foam filter mm membran filter collect filtrat centrifug extract filter transfer pipett ml filtrat ml volumetr flask make volum hydrochlor acid solut mix solut determin strontium part ml volumetr flask transfer pipett ml potassium chlorid solut aliquot wba ml dilut filtrat give expect concentr mg barium millilitr aliquot ml satisfactori start point make volum hydrochlor acid solut mix determin barium concentr solut atom absorpt spectrometri dai condit atom absorpt spectrometri flame nitrou oxid acetylen wavelength nm background correct fuel condit lean maximum absorb optim burner height fuel condit calibr seri ml volumetr flask transfer pipett ml barium standard solut flask transfer pipett ml potassium chlorid solut make volum hydrochlor acid solut mix solut mg barium millilitr similarli prepar blank solut omit barium standard solut measur absorb blank solut obtain barium concentr calibr curv measur absorb barium calibr standard plot calibr curv relat absorb valu barium concentr determin measur absorb sampl solut calibr curv read concentr barium absorb obtain sampl solut calcul solubl barium content pigment formula solubl barium wba mass gram sampl analysi concentr barium sampl solut microgram millilitr obtain calibr curv total volum extract millilitr wba volum extract millilitr repeat estim repeat iso method solubl barium content remark condit barium absorb enhanc presenc calcium counter addit magnesium ion concentr litr induct coupl plasma optic emiss spectrometri permit altern flame atom absorpt spectrometri determin solubl strontium scope field applic method describ procedur extract determin solubl strontium pigment form salt lake principl pigment extract hydrochlor acid solut defin condit amount strontium extract determin atom absorpt spectrometri reagent reagent analyt puriti ethanol absolut hydrochlor acid solut potassium chlorid solut dissolv potassium chlorid ml hydrochlor acid solut strontium standard solut stock strontium standard solut mg ml nitric acid solut spectrosol equival strontium standard solut mg ml transfer pipett ml stock strontium standard solut ml volumetr flask make volum hydrochlor acid solut mix apparatu normal laboratori equip membran filter pore size mm atom absorpt spectrophotomet equip strontium hollow cathod lamp procedur sampl prepar solut prepar determin solubl strontium content ml volumetr flask transfer pipett ml potassium chlorid solut aliquot wsr ml dilut filtrat give expect concentr mg strontium millilitr aliquot ml satisfactori start point make volum hydrochlor acid solut mix determin strontium concentr solut atom absorpt spectrometri dai condit atom absorpt spectrometri flame nitrou oxid acetylen wavelength nm background correct fuel condit lean maximum absorb optim burner height fuel condit calibr seri ml volumetr flask transfer pipett ml strontium standard solut flask transfer pipett ml potassium chlorid solut make volum hydrochlor acid solut mix solut mg strontium millilitr similarli prepar blank solut omit strontium standard solut measur absorb blank solut obtain strontium concentr calibr curv measur absorb strontium calibr standard plot calibr curv relat peak absorb valu strontium concentr determin measur absorb sampl solut calibr curv read concentr strontium absorb obtain sampl solut calcul solubl strontium content pigment formula solubl strontium wsr mass gram sampl analysi concentr strontium sampl solut microgram millilitr obtain calibr curv volum extract millilitr wsr volum extract millilitr repeat estim repeat iso method solubl strontium content remark induct coupl plasma optic emiss spectrometri permit altern flame atom absorpt spectrometri identif determin benzyl alcohol cosmet product identif scope field applic method describ identif benzyl alcohol cosmet product principl benzyl alcohol identifi mean thin layer chromatographi silica gel plate reagent reagent analyt puriti benzyl alcohol chloroform ethanol absolut pentan develop solvent diethyl ether standard solut benzyl alcohol weigh benzyl alcohol ml volumetr flask make volum ethanol mix thin layer chromatographi plate glass mm mm coat mm layer silica gel visual agent molybdophosphor acid ethanol apparatu normal apparatu thin layer chromatographi chromatographi tank doubl trough chamber dimens approxim mm mm mm chromatographi paper whatman equival ultra violet lamp wavelength nm procedur sampl prepar weigh product analys ml volumetr flask add ml chloroform shake vigor product dispers make volum ethanol shake vigor produc clear clear solut thin layer chromatographi satur chromatographi tank pentan line wall chamber adjac back chromatographi paper ensur lower edg paper trough transfer ml pentan back trough pour solvent expos surfac chromatographi paper line immedi replac lid tank stand minut deposit ml sampl solut ml benzyl alcohol standard solut suitabl point start line thin layer chromatographi plate dry pipett ml diethyl ether front tank immedi place plate trough quickli replac lid tank develop plate distanc mm remov plate chromatographi tank dry room temperatur observ plate ultra violet light mark posit violet spot sprai plate visual agent heat plate minut benzyl alcohol appear dark blue spot calcul rf obtain benzyl alcohol standard solut dark blue spot rf obtain sampl solut presenc benzyl alcohol detect limit mg benzyl alcohol determin scope field applic method describ determin benzyl alcohol cosmet product definit amount benzyl alcohol determin method express percentag mass principl sampl extract methanol amount benzyl alcohol extract determin high perform liquid chromatographi hplc reagent reagent analyt puriti suitabl hplc methanol ethoxyphenol benzyl alcohol mobil phase methanol water benzyl alcohol stock solut weigh accur approxim benzyl alcohol ml volumetr flask make volum methanol mix intern standard stock solut weigh accur approxim ethoxyphenol ml volumetr flask make volum methanol mix standard solut seri ml volumetr flask transfer pipett amount benzyl alcohol stock solut intern standard stock solut tabl set make volum methanol mix tabl oj normal laboratori equip high perform chromatographi equip variabl wavelength ultra violet detector ml inject loop analyt column mm mm stainless steel column pack mm spherisorb od equival water bath ultrason bath centrifug centrifug tube ml capac procedur sampl prepar weigh accur approxim gram sampl centrifug tube add ml methanol heat minut water bath maintain place tube ultrason bath sampl dispers cool centrifug rpm minut transfer supernat liquid ml volumetr flask extract sampl ml methanol combin extract ml volumetr flask transfer pipett ml intern standard stock solut ml volumetr flask make volum methanol mix solut determin stage analysi chromatographi set high perform liquid chromatographi equip usual manner adjust flow rate mobil phase ml minut set wavelength uv detector nm calibr inject ml benzyl alcohol standard solut measur area benzyl alcohol ethoxyphenol peak benzyl alcohol standard solut calcul peak area ratio benzyl alcohol ethoxyphenol plot calibr curv ratio ordin concentr benzyl alcohol mg millilitr abscissa determin inject ml sampl solut measur area benzyl alcohol ethoxyphenol peak calcul peak area ratio benzyl alcohol ethoxyphenol repeat process ml aliquot sampl solut consist result obtain calibr curv read concentr benzyl alcohol peak area ratio benzyl alcohol ethoxyphenol calcul calcul benzyl alcohol content sampl percentag mass formula benzyl alcohol mass gram sampl analysi concentr benzyl alcohol sampl solut microgram millilitr obtain calibr curv repeat benzyl alcohol content differ result determin carri parallel sampl exce identif zirconium determin zirconium aluminium chlorin aerosol antiperspir method compris stage identif zirconium determin zirconium determin aluminium determin chlorin calcul ratio aluminium atom zirconium atom aluminium zirconium atom chlorin atom identif zirconium scope field applic method describ identif zirconium aerosol antiperspir cosmet product attempt made describ method suitabl identif aluminium zirconium chlorid hydroxid complex alxzr yclz nh principl zirconium identifi characterist red violet precipit produc alizarin red strongli acid condit reagent reagent analyt puriti hydrochlor acid concentr ml alizarin red ci solut aqueou sodium alizarin sulphon apparatu normal laboratori equip procedur sampl test tube add ml water stopper shake add drop alizarin red solut ml concentr hydrochlor stopper shake leav stand approxim minut red violet colour supernat solut precipit presenc zirconium determin zirconium scope field applic method suitabl determin zirconium aluminium zirconium chlorid hydroxid complex maximum concentr zirconium aerosol antiperspir principl zirconium extract product acid condit determin flame atom absorpt spectrometri reagent reagent analyt puriti hydrochlor acid concentr ml hydrochlor acid solut add ml concentr hydrochlor acid ml water beaker stir continu transfer solut litr volumetr flask make volum water stock zirconium standard solut mg ml hydrochlor acid solut spectrosol equival aluminium chlorid hydrat alcl reagent dissolv aluminium chlorid hexahydr ml hydrochlor acid solut ammonium chlorid reagent dissolv ammonium chlorid ml hydrochlor acid solut apparatu normal laboratori equip heater magnet stirrer filter paper whatman equival atom absorpt spectrophotomet equip zirconium hollow cathod lamp procedur sampl prepar weigh accur approxim gram homogen sampl product ml beaker add ml water ml concentr hydrochlor acid place beaker heater magnet stirrer commenc stir heat boil prevent rapid dry place watch glass top beaker boil minut remov beaker heat cool room temperatur filter paper filter content beaker ml volumetr flask rins beaker ml portion water add wash filtrat flask make volum water mix solut determin aluminium part ml volumetr flask transfer pipett ml sampl solut ml aluminium chlorid reagent ml ammonium chlorid reagent make volum hydrochlor acid solut mix condit atom absorpt spectrometri flame nitrou oxid acetylen wavelength nm background correct fuel condit rich maximum absorb optim burner height fuel condit calibr seri ml volumetr flask transfer pipett ml stock zirconium standard solut volumetr flask transfer pipett ml aluminium chlorid reagent ml ammonium chlorid reagent make volum hydrochlor acid solut mix solut mg zirconium millilitr similarli prepar blank solut omit zirconium standard solut measur absorb blank solut obtain zirconium concentr calibr curv measur absorb zirconium calibr standard plot calibr curv relat absorb valu zirconium concentr determin measur absorb sampl solut calibr curv read concentr zirconium absorb obtain sampl solut calcul calcul zirconium content sampl percentag mass formula zirconium mass gram sampl analysi concentr zirconium sampl solut microgram millilitr obtain calibr curv repeat zirconium content differ result determin carri parallel sampl exce remark induct coupl plasma optic emiss spectrometri permit altern flame atom absorpt spectrometri determin aluminium scope field applic method suitabl determin aluminium present aluminium zirconium chlorid hydroxid complex maximum concentr aluminium aerosol anti perspir principl aluminium extract product acid condit determin flame atom absorpt sepctrometri reagent reagent analyt puriti hydrochlor acid concentr ml hydrochlor acid solut add ml concentr hydrochlor acid ml water beaker stir continu transfer solut litr volumetr flask make volum water stock aluminium standard solut mg ml nitric acid solut spectrosol equival potassium chlorid reagent dissolv potassium chlorid ml hydrochlor acid solut apparatu normal laboratori equip atom absorpt spectrophotomet equip aluminium hollow cathod lamp procedur sampl prepar solut prepar determin aluminium content ml volumetr flask transfer pipett ml sampl solut ml potassium chlorid reagent make volum hydrochlor acid solut mix condit atom absorpt spectrometri flame nitrou oxid acetylen wavelenght nm background correct fuel condit rich maximum absorb optim burner height fuel condit calibr seri ml volumetr flask transfer pipett ml stock aluminium standard solut volumetr flask transfer pipett ml potassium chlorid reagent make volum hydrochlor acid solut mix solut mg aluminium millilitr similarli prepar blank solut omit aluminium standard solut measur absorb blank solut obtain aluminium concentr calibr curv measur absorb aluminium calibr standard plot calibr curv relat absorb valu aluminium concentr determin measur absorb sampl solut calibr curv read concentr aluminium absorb obtain sampl solut calcul calcul aluminium content sampl percentag mass formula aluminium mass gram sampl analysi concentr aluminium sampl solut microgram millilitr obtain calibr curv repeat aluminium content differ result determin carri parallel sampl exce remark induct coupl plasma optic emiss spectrometri permit altern flame atom absorpt spectrometri determin chlorin scope field determin method suitabl determin chlorin present chlorid ion aluminium zirconium chlorid hydroxid complex aerosol anti perspir principl chlorid ion product determin potentiometr titrat standard silver nitrat solut reagent reagent analyt puriti nitric acid concentr ml nitric acid solut add ml concentr nitric acid ml water beaker stir continu transfer solut ml volumetr flask make volum water aceton silver nitrat volumetr solut analar equival apparatu normal laboratori equip heater magnet stirrer silver electrod calomel refer electrod ph millivolt meter suitabl potentiometr titrat procedur sampl prepar weigh accur ml beaker approxim gram homogen sampl product add ml water ml nitric acid solut place beaker heater magnet stirrer commenc stir heat boil prevent rapid dry place watch glass top beaker boil minut remov beaker heat cool room temperatur add ml aceton dip electrod surfac solut commenc stir titrat potentiometr silver nitrat solut plot differenti curv determin endpoint ml calcul calcul chlorin content sampl percentag mass formula chlorin mass gram sampl analysi volum silver nitrat millilitr titrat endpoint repeat chlorin content differ result determin carri parallel sampl exce calcul ratio aluminium atom zirconium atom aluminium zirconium atom chlorin atom calcul ratio aluminium atom zirconium atom calcul al zr ratio formula al zr ratio al zr calcul ratio aluminium zirconium atom chlorin atom calacul al zr cl ratio formula al zr al zr cl ratio cl identif determin hexamidin dibromohexamidin dibromopropamidin chlorhexidin scope field applic method describ qualit quantit determin hexamidin salt includ isethion hydroxybenzo dibromohexamidin salt includ isethion dibromopropamidin salt includ isethion chlorhexidin diacet diglucon dihydrochlorid cosmet product definit concentr hexamidin dibromohexamidin dibromopropamidin chlorhexidin determin method express percentag mass principl identif determin carri ion pair revers phase high perform liquid chromatographi hplc ultra violet spectrophotometr detect hexamidin dibromohexamidin dibromopropramidin chlorhexidin identifi retent time chromatograph column reagent reagent analyt puriti suitabl hplc methanol heptanesulphon acid sodium salt monohydr acet acid glacial ml sodium chlorid mobil phase solvent solut heptanesulphon acid sodium salt monohydr methanol adjust appar ph glacial acet acid solvent ii solut heptanesulphon acid sodium salt monohydr water adjust ph glacial acet acid note improv shape peak mobil phase modifi prepar solvent dissolv sodium chlorid heptanesulphon acid sodium salt monohydr ml water add ml methanol adjust appar ph glacial acet acid solvent ii dissolv sodium chlorid heptanesulphon acid sodium salt monohydr litr water adjust ph glacial acet acid hexamidin diisethion dibromohexamidin diisethion br dibromopropamidin diisethion br chlorhexidin diacet cl refer solut prepar solut preserv solvent amp prime trichlorocarbanilid triclocarban amp prime dichloro trifluoromethyl carbanilid halocarban apparatu normal laboratori equip high perform liquid chromatograph variabl wavelength uv detector analyt column stainless steel length cm intern diamet mm pack bondapack mm equival ultrason bath identif sampl prepar weigh approxim sampl ml volumetr flask make volum solvent place flask ultrason bath minut filter centrifug solut collect filtrat supernat chromatographi chromatographi mobil phase gradient temperatur set detector wavelength nm inject ml refer solut record chromatogram inject ml sampl solut record chromatogram identifi hexamidin dibromohexamidin dibromopropamidin chlorhexidin present compar retent time peak record obtain refer solut determin determin prepar standard solut preserv absent sampl intern standard triclocarban halocarban stock solut solvent preserv identifi stock solut solvent preserv chosen intern standard identifi preserv prepar standard solut transfer seri ml volumetr flask amount stock solut identifi preserv amount stock solut intern standard tabl set make flask volum solvent mix tabl oj weigh accur approxim gram sampl ml volumetr flask add ml intern standard solut ml solvent mix place flask ultrason bath minut cool make volum solvent mix centrifug filter fold filter paper collect supernat filtrat case chromatographi chromatographi adjust mobil phase gradient flow rate column temperatur detector wavelength hplc equip condit requir identif stage inject ml sampl solut measur peak area repeat process ml aliquot sampl solut consist result obtain calcul ratio peak area produc compound analys peak area produc intern standard calibr inject ml standard solut measur peak area standard solut calcul ratio hexamidin dibromohexamidin dibromopropamidin chlorhexidin peak area intern standard peak area plot calibr curv ratio ordin concentr identifi preserv standard solut microgram millilitr abscissa calibr curv read concentr identifi preserv peak area ratio calcul calcul calcul hexamidin dibromohexamidin dibromopropamidin chlorhexidin content sampl percentag mass formula mw mw mass gram sampl analysi concentr preserv sampl solut microgram millilitr obtain calibr curv mw molecular weight basic form preserv present mw molecular weight salt point repeat hexamidin dibromohexamidin dibromopropamidin chlorhexidin concentr differ result determin carri parallel sampl exce tabl formula weight hexamidin hexamidin diisethion hexamidin di hydroxybenzo dibromohexamidin br dibromohexamidin diisethion br dibromopropamidin br dibromopropamidin diisethion br chlorhexidin cl chlorhexidin diacet cl chlorhexidin diglucon cl chlorhexidin dihydrochlorid cl hcl iso iso magnesium modifi determin barium flame atom emiss spectrometri jerrow al analyt proceed iso iso iso iso iso