commiss regul eec juli characterist oliv oil oliv residu oil relev method analysi commiss european commun regard treati establish european econom commun regard council regul eec septemb establish common organ market oil fat amend regul eec articl thereof annex regul eec descript definit oliv oil oliv residu oil market member state intra commun trade trade countri purpos differenti type oil physic chemic characterist organolept characterist virgin oil defin order guarante puriti qualiti product concern prejudic exist provis presenc characterist type oil determin uniformli commun end commun method chemic analysi organolept evalu establish permit transit period method analysi appli member state provid differ result obtain common method decis definit physic chemic characterist oliv oil method analysi entail amend addit note chapter combin nomenclatur method evalu organolept characterist virgin oil includ set panel select train taster period establish structur fix view difficulti member state encount set panel taster panel member state author order ensur system levi applic import oliv residu function correctli singl method determin oil content product laid order harm trade provis made oil packag prior entri forc regul dispos limit period repeal commiss regul eec amend regul eec manag committe oil fat deliv opinion time limit set chairman adopt regul articl oil characterist compli set point annex regul deem virgin oliv oil mean point annex regul eec oil characterist compli set point annex regul deem lampant virgin oliv oil mean point annex regul eec oil characterist compli set point annex regul deem refin oliv oil mean point annex regul eec oil characterist compli set point annex regul deem pure oliv oil mean point annex regul eec oil characterist compli set point annex regul deem oliv residu oil mean point annex regul eec oil characterist compli set point annex regul deem refin oliv residu oil mean point annex regul eec oil characterist compli set point annex regul deem oliv residu oil mean point annex regul eec articl characterist oil laid annex determin accord method analysi set determin free fatti acid express percentag oleic acid method set annex ii determin peroxid index method set annex iii determin aliphat alcohol method set annex iv determin sterol content method set annex determin erythrodiol uvaol method set annex vi determin satur fatti acid posit triglycerid method set annex vii determin trilinolein content method set annex viii spectrophotometr analysi method set annex ix determin fatti acid composit method set annex determin volatil halogen solvent method set annex xi evalu organolept characterist virgin oliv oil method set annex xii proof refin place method set annex xiii evalu organolept characterist carri analyst assist specialist procedur tast note refer annex xii analysi show characterist result descript product sampl examin panel taster accord provis annex xii analysi carri panel provis order ascertain organolept characterist connect oper relat intervent system panel taster carri evalu accord provis annex xii articl octob introduct analysi method provid articl imped member state test scientif valid method provid product recogn compli rule forc govern commun method allow move freeli method member state concern notifi commiss method produc result produc common method result obtain method determin articl purpos assess organolept characterist member state set panel train select taster accord rule laid method set annex xii member state encount difficulti set panel territori servic panel oper member state articl addit note chapter combin nomenclatur replac contain annex xiv articl oil content oil cake residu result extract oliv oil cn code determin method set annex xv oil content refer paragraph express percentag weight oil weight dry matter articl commun provis presenc undesir substanc refer annex xi appli articl member state notifi commiss measur implement regul member state send commiss begin half year statement analyt data relat test carri previou half year result consid manag committe oil fat accord procedur laid articl regul eec articl regul eec repeal articl regul enter forc dai public offici journal european commun method set annex xii appli januari oper relat intervent system concern regul appli oliv oil oliveresidu oil packag entri forc regul market octob regul bind entireti directli applic member state brussel juli commiss rai mac sharri member commiss oj oj oj oj annex summari page annex characterist oliv oil annex ii determin free fatti acid annex iii determin peroxid annex iv determin aliphat alcohol content capillari ga chromatographi annex determin composit content sterol capillari column ga chromatographi annex vi determin erythrodiol uvaol annex vii determin satur fatti acid posit triglycerid annex viii determin composit trilinolein annex ix spectrophotometr investig ultraviolet annex xa analysi ga chromatographi methyl ester fatti acid annex xb prepar methyl ester fatti acid annex xi determin volatil halogen solvent oliv oil annex xii organolept assess virgin oliv oil annex xiii proof refin place annex xiv addit note chapter combin nomenclatur annex xv oil content oliv residu annex xvi determin iodin annex characterist oliv oil gt tabl gt gt tabl gt annex ii determin free fatti acid determin acid determin free fatti acid oliv oil content free fatti acid express acid calcul convention principl sampl dissolv mixtur solvent free fatti acid present titrat ethanol solut potassium hydroxid reagent reagent recogn analyt qualiti water distil equival puriti diethyl oxid ethanol mixtur equal part volum note diethyl oxid highli inflamm form explos peroxid special care neutral precis moment potassium hydroxid solut addit ml phenolpthalein solut ml mixtur note diethyl oxid mixtur solvent ethanol toluen ethanol replac propanol potassium hydroxid titrat ethanol solut koh mol koh mol exact concentr ethanol solut potassium hydroxid check immedi prior solut prepar dai decant brown glass bottl rubber stopper solut colourless straw colour note stabl colourless solut potassium hydroxid prepar bring boil ml ethanol potassium hydroxid aluminium shave continu boil reflux hour distil immedi dissolv distil requir quantiti potassium hydroxid leav dai decant clear supernat liquid precipit potassium carbon solut prepar distil ml ethanol add ml aluminium butyl leav mixtur dai decant supernat liquid dissolv requir quantiti potassium hydroxid solut readi phenolphthalein solut ethanol alkalin blue case strongli colour fat solut ethanol apparatu usual laboratori equip includ analyt balanc ml conic flask ml burett graduat ml procedur prepar specimen test carri test filter sampl moistur impur specimen treatment exce filter take sampl sampl depend presum acid number accord tabl gt tabl gt weigh sampl conic flask determin dissolv sampl ml previous neutral mixtur diethyl oxid ethanol titrat stir mol solut potassium hydroxid note indic pink colour phenolphtalein persist second note titrat ethanol solut potassium hydroxid replac aqueou solut potassium sodium hydroxid provid volum water introduc induc phase separ note quantiti mol potassium hydroxid solut requir exce ml mol solut note solut cloudi titrat add solvent give clear solut acid express percentag oleic acid acid percentag weight equal volum titrat potassium hydroxid solut millilitr exact concentr mole litr titrat solut potassium hydroxid molar weight gram mole acid express result weight gram sampl arithmet calcul result annex iii determin peroxid scope standard describ method determin peroxid oil fat field applic standard applic anim veget oil fat definit peroxid quantiti substanc sampl express term milliequival activ oxygen kilogram oxid potassium iodid oper condit principl treatment test portion solut acet acid chloroform solut potassium iodid titrat liber iodin standard sodium thiosulph solut apparatu equip free reduc oxid substanc note greas ground surfac ml glass scoop flask ground neck stopper ml capac dri fill pure dry inert ga nitrogen prefer carbon dioxid ml burett graduat ml reagent chloroform analyt reagent qualiti freed oxygen bubbl current pure dry inert ga glacial acet acid analyt reagent qualiti freed oxygen bubbl current pure dry ga potassium iodid satur aqueou solut recent prepar free iodin iodat sodium thiosulph accur standard aqueou solut standard starch solut aqueou dispers recent prepar natur solubl starch sampl care sampl store light cold contain complet fill glass contain hermet seal ground glass cork stopper procedur test carri diffus daylight artifici light weigh glass scoop fail flask nearest mass sampl accord tabl expect peroxid gt tabl gt unstopp flask introduc glass scoop test portion add ml chloroform dissolv test portion rapidli stir add ml acet acid ml potassium iodid solut insert stopper quickli shake minut leav minut light temperatur oc add ml distil water titrat liber iodin sodium thiosulph solut solut expect valu solut expect valu shake vigor starch solut indic carri determin test sampl carri simultan blank test result blank exce ml sodium thiosulph solut replac impur reagent express result peroxid pv express milliequival activ oxygen kilogram formula pv number ml standard sodium thiosulph solut test correct account blank test exact normal sodium thiosulph solut weight test portion result arithmet determin carri annex iv determin aliphat alcohol content capillari ga chromatographi object procedur describ method determin eliphat alcohol content oil fat principl method fatti substanc eicosanol ad intern standard saponifi methanol potassium hydroxid unsaponifi matter extract ethyl ether alcohol fraction separ unsaponifi matter chromatographi layer potassium hydroxid impregn silica gel alcohol recov silica gel transform trimethylsilyl ether analyz capillari ga chromatographi apparatu ml round bottom flask reflux condens ground glass joint separ funnel ml capac flask ml capac chromatograph tank thin layer chromatograph analysi glass plate dimens cm uv light wavelenght nm examin tlc plate microsyring deliv ml ml sinter glass filter funnel porou frit poros approxim diamet cm approxim height cm suitabl filter vacuum ground male joint vacuum flask ml capac ground glass femal joint filter funnel test tube ml capac conic bottom plug ga chromatograph capillari column provid split system compos thermostat chamber column column oven hold temperatur desir precis oc thermostat vapor assembl inject port silanizet coat glass flame ioniz detector convert amplifi record integr oper convert amplifi respons time exceed variabl paper speed glass fuse silica capillari column length intern diamet mm se se liquid phase equival film thick mm microsyring ga chromatographi ml capac harden needl reagent potassium hydroxid approxim ethanol solut potassium hydroxid minimum concentr dissolv cool ml distil water made litr ethanol solut store stopper dark colour glass bottl ethyl ether pure analysi anhydr sodium sulphat pure analysi silica gel tlc glass plate floresc indic thick mm obtain commerci prepar potassium hydroxid approxim ethanol solut potassium hydroxid dissolv ml distil water made litr ethanol benzen chromatographi aceton chromatographi hexan chromatographi ethyl ether chromatographi chloroform chromatographi refer solut thin layer chromatographi mixtur alcohol chloroform solut dichlorofluorescein ethanol made slightli basic ad drop potassium hydroxid solut anhydr pyridin chromatographi hexamethyldisilazan trimethylchlorosilan standard solut trimethylsilyl ether aliphat alcohol prepar mixtur pure alcohol time requir solut eicosanol chcl intern standard carrier gase hydrogen helium pure ga chromatographi auxiliari gase hydrogen pure ga chromatographi air pure ga chromatographi procedur prepar unsaponifi matter ml microsyring place ml round bottom flask volum eicosanol eneicosanol solut quantiti eicosanol approxim equal aliphat alcohol content portion sampl analysi sampl add ml eicosanol solut oliv oil seed oil ml oliv residu oil evapor intern standard solut dryness quantit weigh flask approxim dry filter sampl ml potassium hydroxid ethanol solut ad reflux condens fit apparatu heat slight boil steam bath stir continu heat process saponif place solut clear heat continu minut ml distil water ad condens condens diconnect flask cool approxim oc content flask quantit transfer separ funnel ml capac aid ml distil water approxim ml ethyl ether ad shaken vigor second left stratifi note aqueou phase beneath transfer separ funnel extract effect aqueou phase manner time ml ethyl ether note emuls elimin ad sprai small quantiti ethyl alcohol methyl alcohol ethyl ether extract combin separ funnel wash distil water ml time wash water neutral reaction discard aqueou phase dry anhydr sodium sulphat filter flask ml capac weigh funnel filter wash small quantiti ethyl ether ad total ether evapor gentl geat ml dri slight vacuum current nitrogen dry finish oven oc approxim minut residu weigh cool dessic separ alcohol fraction prepar basic tlc plate immers complet potassium hydroxid solut second left dry hood hour final oven oc hour plate oven store calcium chlorid dessic requir plate treat week note basic silica gel plate separ alcohol fraction unsaponifi substanc treat acid compound fatti acid retain origin obtain aliphat alcohol terpen alcohol band separ distinctli sterol band solut volum benzen aceton introduc develop chamber approxim depth cm altern volum mixtur hexan ethyl ether tank close left half hour equilibr vapour liquid strip filter paper dip eluent affix insid surfac tank reduc develop time approxim obtain uniform regular elut compon note develop solut replac analysi order obtain reproduc develop condit approxim solut unsaponifi matter chloroform prepar ml solut streak uniform strip minimum thick mean microsyring ml capac tlc plate approxim cm bottom tlc plate align origin ml aliphat alcohol refer solut spot identif aliphat alcohol band develop complet plate insid develop tank state temperatur oc tank close immedi sampl left elut front solvent reach distanc cm top plate plate remov develop tank solvent evapor hot air current plate left hood plate sprai lightli evenli solut dichlorofluorescein plate observ ultra violet light aliphat alcohol band identifi comparison aliphat alcohol band immedi triterpen alcohol band outlin note requir group aliphat alcohol band triterpen alcohol band determin migrat aliphat alcohol triterpen alcohol band silica gel enclos area defin scrape metal spatula materi remov broken fine fragment introduc filter funnel ml hot chloroform ad content mix metal spatula filter vacuum filtrat collect flask connect filter funnel residu insid funnel wash ml ethyl ether collect filtrat flask fit funnel filtrat evapor volum approxim ml residu solut pour test tube ml capac weigh test tube dri light heat gentl nitrogen current redissolv residu drop aceton dry place oven oc minut remov cool dessic weigh residu insid test tube compos alcohol fraction prepar trimethylsili ether reagent silyl consist mixtur volum note pyridinehexamethyldisilazan trimethylchlorosilan proport ml milligram alcohol ad test tube alcohol fraction avoid absorpt moistur note note solut readi commerci silan reagent bi trimethylsilyl trifluoroacetamid trimethylchlorosilan mix volum anhydr pyridin test tube stopper shaken carefulli overturn alcohol solubil left minut room temperatur subsequ centrifug minut clear solut readi ga chromatograph analysi note format slight opalesc normal interfer format white floccul appear pink colour sign presenc moistur deterior reagent case test repeat ga chromatographi analysi preliminari oper condit capillari column capillari column fit insid ga chromatograph connect begin column evapor connect split system end column detector gener check ga chromatographi assembl perform tight ga fit effici detector effici split system record system capillari column time condit carrier ga caus flow capillari column ga chromatographi assembl switch gradual heat accomplish temperatur oc oper temperatur note attain temperatur held hour assembl brought oper condit regul ga flow split flame ignit connect electron record adjust temperatur capillari column oven detector injector signal adjust sensit highest level contempl execut analysi base line trace linear devoid spike natur show sign drift neg rectilinear drift indic imperfect tight column connect posit drift indic insuffici condit column note temperatur condit oc maximum temperatur contempl liquid phase emploi select oper condit gener oper condit column temperatur initi isotherm set oc minut program oc minut oc minut oc temperatur evapor oc temperatur detector oc linear veloc carrier ga helium cm hydrogen cm split ratio sensit instrument time minimum attenu sensit record mv fs paper speed cm quantiti substanc inject ml tmse solut condit modifi characterist column ga chromatograph order chromatogram satisfi condit obtain alcohol retent time minut alcohol peak full scale oliv oil full scale seed oil aforesaid requir check repeat inject standard tmse mixtur alcohol oper condit adjust yield result paramet integr peak set correct apprais area peak consid obtain execut analysi microsyring ml capac draw ml hexan ml air subsequ ml sampl solut plunger microsyring rais empti needl needl introduc septum inject assembl second solut inject rapidli needl extract slowli approxim second record effect tmse alcohol present elut complet base line correspond requir identif peak identif individu peak effect retent time comparison standard tmse mixtur analyz condit chromatogram alcohol fraction virgin oliv oil shown figur quantit apprais peak area eicosanol aliphat alcohol calcul electron integr content alcohol express mg fatti substanc calcul alcohol ax ms alcohol ax ms ax area alcohol peak squar millimetr area eicosanol squar millimetr ms mass eicosanol milligram mass sampl drawn determin gram express result content individu aliphat alcohol mg fatti substanc sum total aliphat alcohol report appendix determin linear veloc ga ìl methan propan inject ga chromatograph set normal oper condit time methan propan flow column instant inject instant peak elut tm measur stop clock linear veloc cm tm length column centimetr tm time second measur stop clock gt start graphic gt figur chromatogram alcohol fraction virgin oliv oil eicosanol si decosanol tricosanol tetracosanol pentacosanol hexacosanol heptacosanol octacosanol gt end graphic gt annex determin composit content sterol capillari column ga chromatographi scope method describ procedur determin individu total sterol content fatti substanc principl method fatti substanc ad cholestanol intern standard saponifi potassium hydroxid ethanol solut unsaponifi extract ethyl ether sterol fraction separ unsaponifi extract chromatographi basic silica gel plate sterol recov silica gel transform trimethyl silyl ether analys capillari column ga chromatographi apparatu ml flask fit reflux condens ground glass joint ml separ funnel ml flask complet apparatu analysi thin layer chromatographi cm glass plate ultraviolet lamp wavelength nm ml ml microsyring cylindr filter funnel porou septum poros mm diamet approxim cm depth cm attach suitabl filtrat vacuum male ground glass joint ml vacuum conic flask ground glass femal joint fit filter funnel ml test tube taper bottom seal stopper ga chromatograph suitabl capillari column provid split system consist thermostat chamber column capabl maintain desir temperatur accuraci oc temperatur adjust vapor unit persilan glass vapour element flame ioniz detector convert amplifi integr record suitabl convert amplifi respons time variabl paper speed glass fuse silica capillari column length intern diamet mm coat se se liquid equival uniform thick mm ml ga chromatographi microsyring harden needl reagent potassium hydroxid approxim ethanol solut dissolv potassium hydroxid minimum titl cool ml distil water make litr ethanol solut stopper dark glass bottl ethyl ether analyt puriti anhydr sodium sulphat analyt puriti glass plate coat silica gel fluoresc indic thick mm commerci readi potassium hydroxid ethanol solut dissolv potassium hydroxid ml distil water make litr ethanol benzen chromatographi aceton chromatographi hexan chromatographi ethyl ether chromatographi chloroform analyt puriti refer solut thin layer chromatographi cholesterol phytosterol solut chloroform dichlorofluorescein ethanol solut make slightli basic ad drop alcohol potassium hydroxid solut anhydr pyridin chromatographi hexamethyl disilazan trimethylchlorosilan refer solut sterol trimethylsilyl ether prepar time pure sterol mixtur sterol obtain oil cholestanol solut chloroform intern standard carrier ga hydrogen helium ga chromatograph puriti auxiliari gase hydrogen ga chromatograph puriti air ga chromatograph puriti procedur prepar unsaponifi ml microsyring introduc volum cholestanol solut chloroform amount cholestanol approxim sterol content sampl aliquot determin ml flask sampl add ml cholestanol solut case oliv oil ml seed oil oliv pomaca oil evapor dryness current nitrogen weigh accur dry filter sampl flask anim veget oil fat appreci quantiti cholesterol show peak retent time ident cholestanol occur sterol fraction analyz duplic intern standard add ml ethanol potassium hydroxid solut fit reflux condens heat gentl boil water bath continu vigor stir saponif take place solut clear continu heat minut add ml distil water top condens detach condens cool flask approxim oc transfer content flask quantit ml separ funnel rins distil water amount ml add approxim ml ethyl ether shake vigor approxim second settl note separ lower aqueou phase collect separ funnel perform extract aqueou phase ml ethyl ether occas note emuls destroi ad small quantiti ethyl methyl alcohol mean sprai pool ether extract singl separ funnel wash distil water ml time wash water neutral reaction wash water remov dry anhydr sodium sulphat filter anhydr sodium sulphat previous weigh ml flask wash funnel filter small quantiti ethyl ether distil ether ml bring dryness slight vacuum current nitrogen complet dry stove oc approxim quarter hour weigh cool desicc separ sterol fraction prepar basic plate immers silica gel plate complet ethanol potassium hydroxid solut second dry fume cupboard hour final place stove oc hour remov stove calcium chlorid desicc requir plate treat dai note basic silica gel plate separ sterol fraction treat unsaponifi alumina compound acid natur fatti acid retain spot line sterol band separ aliphat triterpen alcohol band place benzen aceton mixtur plate develop chamber depth approxim cm altern hexan ethyl ether mixtur close chamber cover leav approxim half hour liquid vapour equilibrium establish strip filter paper dip eluent intern surfac chamber reduc develop time approxim bring uniform regular elut compon note develop mixtur replac test order achiev perfectli reproduc elut condit prepar approxim solut unsaponifi chloroform ml microsyring streak chromatograph plate ml approxim cm end streak thin uniform line streak place ml sterol refer solut end plate sterol band identifi develop place plate develop chamber prepar ambient temperatur maintain oc immedi close chamber cover elut solvent front reach approxim cm upper edg plate remov plate develop chamber evapor solvent flow hot air leav plate short hood sprai plate lightli uniformli dichlorofluoroscein solut plate observ ultraviolet light sterol band identifi align stain obtain refer solut mark limit band edg fluoresc black pencil metal spatula scrape silica gel mark area place fine comminut materi remov filter funnel add ml hot chloroform mix carefulli metal spatula filter vacuum collect filtrat conic flask attach filter funnel wash residu flask time ethyl ether approxim ml time collect filtrat flask attach funnel evapor filtrat volum ml transfer residu solut previous weigh ml test tube evapor dryness mild heat gentl flow nitrogen make drop aceton evapor dryness place stove oc approxim minut cool desicc weigh residu contain test tube consist sterol fraction prepar trimethylsilyl ether add silyl reagent consist mixtur pyridin hexamethyl disilazan trimethyl chlorosilan note ratio ml milligram sterol test tube sterol fraction avoid uptak moistur note note solut readi commerci silan reagent bi trimethylsilyl trifluor acetamid trimethyl chlorosilan dilut equal volum anhydr pyridin stopper test tube shake carefulli overturn sterol complet dissolv stand minut ambient temperatur centrifug minut clear solut readi ga chromatograph analysi note slight opalesc form normal interfer format white floc appear pink colour indic presenc moistur deterior reagent occur test repeat ga chromatograph analysi preliminari oper column pack fit column ga chromatograph attach inlet end evapor connect split system outlet end detector carri gener check ga chromatograph unit leak ga circuit detector effici effici split system record system column time recommend subject condit pass gentl flow ga column switch ga chromatographi unit begin gradual heat temperatur oc oper temperatur note hold temperatur hour place entir unit oper mode adjust ga flow split ignit flame connect electron record adjust column chamber detector injector temperatur record signal sensit time greater intend analysi base line linear peak kind drift neg straight line drift leakag column connect posit drift inadequ condit column note condit temperatur oc maximum temperatur stationari phase choic oper condit guidelin oper condit column temperatur oc evapor temperatur oc detector temperatur oc linear veloc carrier ga helium cm hydrogen cm split ratio instrument sensit time minimum attenu record sensit mv paper speed cm hour amount substanc inject ml tmse solut condit vari light column ga chromatograph characterist obtain chromatogram meet requir retent time sitosterol minut campesterol peak oliv oil content full scale soya oil content full scale sterol present separ addit separ peak complet resolv peak trace return base line leav peak incomplet resolut toler provid peak trr quantifi perpendicular analyt procedur ml microsyring ml hexan draw ml air ml sampl solut rais plunger syring needl empti push needl membran inject unit second inject rapidli slowli remov needl second continu record tmse sterol present complet elut base line continu meet requir peak identif identifi individu peak basi retent time comparison mixtur sterol tmse analys condit sterol elut order cholesterol brassicasterol methylen cholesterol campesterol campestanol stigmasterol campesterol stigmastadienol clerosterol sistosterol sitostanol avenasterol stigmastadienol sigmastenol avenasterol retent time sitosterol se se column shown tabl figur illustr typic chromatogram oil quantit evalu calcul area cholestanol sterol peak integr ignor peak compound includ list tabl respons coeffici cholestanol equal calcul concentr individu sterol mg fatti materi sterol ax ms sterol ax ms ax peak area sterol squar millimetr area cholestanol peak squar millimetr ms mass cholestanol ad im milligram mass sampl determin gram express result record individu sterol concentr mg fatti materi sum total sterol calcul percentag individu sterol ratio relev peak area total peak area sterol sterol óa sterol ax óa ax peak area óa total peak area sterol appendix determin linear veloc ga ga chromatograph set normal oper condit inject ml methan propan measur time ga pass column time inject time peak appear tm linear veloc cm tm length column centimetr tmi measur time second gt tabl gt gt start graphic gt figur ga chromatogram sterol fraction unrefin oliv oil gt end graphic gt gt start graphic gt figur ga chromatogram sterol fraction refin oliv oil gt end graphic gt annex vi determin erythrodiol uvaol introduct erythrodiol commonli understood glycol erythrodiol uvaol constitu unsaponifi fraction characterist type fatti substanc found consider higher concentr solvent extract oliv oil oil press oliv oil grape pip oil presenc demonstr presenc solvent extract oliv oil scope method describ procedur detect erythrodiol fatti substanc principl method fatti substanc saponifi potassium hydroxid methanol ethanol solut unsaponifi fraction extract ethyl ether purifi passag column alumina unsaponifi subject thin layer chromatographi silica gel plate band sterol erythrodiol fraction separ sterol erythrodiol recov plate transform trimethylsilyl ether mixtur analys ga chromatographi result express percentag erythrodiol mixtur erythrodiol sterol apparatu apparatu annex determin content sterol reagent reagent annex determin content sterol refer solut erythrodiol solut chloroform procedur prepar unsaponifi paragraph annex separ erythrodiol sterol paragraph annex paragraph annex prepar solut unsaponifi chloroform ml microsyring streak chromatograph plate ml solut approxim cm lower edg streak thin uniform end plate place microlitr solut cholesterol erythrodiol serv refer place plate develop chamber prepar ambient temperatur oc immedi close chamber cover elut solvent front reach approxim cm upper edg plate remov plate develop chamber evapor solvent flow hot air sprai plate lightli uniformli alcohol dichlorofluoroscein solut plate observ ultralviolet light sterol erythrodiol band identifi align refer mark spot edg fluoresc metal spatula scrape silica gel mark area place materi plate ml flask add ml hot chloroform shake filter funnel sinter glass disc silica gel transfer filter wash time hot chloroform ml time collect filtrat ml flask evapor filtrat volum ml transfer calibr ml conic bottom centrifug tube dry gentli heat current nitrogen weigh prepar trimethylsili ester paragraph annex ga chromatograph analysi paragraph method oper condit ga chromatograph analysi perform sterol analysi separ tmse erythrodiol uvaol sampl inject continu record sterol present erythrodiol uvaol elut identifi peak retent time erythrodiol uvaol rel sitosterol calcul area sterol express result erythrodiol asterol erythrodiol asterol peak area erythrodiol squar millimetr peak area uvaol squar millimetr asterol total peak area sterol squar millimetr result express decim place annex vii determin fatti acid posit triglycerid oil fat scope standard describ method determin composit fraction fatti acid oil fat esterifi posit intern posit glycerol field applic standard applic oil fat melt point oc ow peculiar action pancreat lipas applic unreservedli oil fat substanti amount fatti acid fewer carbon atom coconut palmkernel oil butterfat highli unsatur fatti acid doubl bound carbon atom fish marin anim oil fatti acid oxygen group acid group principl neutral acid oil fat solvent purif pass alumina column partial hydrolysi triglycerid pancreat lipas determin time separ form monoglycerid thin layer chromatographi methanolysi monoglycerid analysi methyl ester ga liquid chromatographi apparatu ml round bottom flask ml round bottom flask ground joint long air condens fit flask ml conic flask ml beaker ml separ funnel chromatograph glass column mm intern diamet mm length fit frit glass disc tap ml centrifug tube ground glass stopper ml burett graduat ml ml hypoderm syring fit thin needl microsyring deliv drop ml spreader thin layer chromatographi glass plate thin layer chromatographi cm glass develop tank thin layer chromatographi ground glass lid suitabl plate sprai thin layer chromatographi oven regul oc thermostat regulat oc oc rotari evapor vibrat electr shaker allow vigor agit centrifug tube ultraviolet lamp examin thin layer plate control lipas activ ph meter spiral stirer ml burett stop watch prepar lipas laboratori stirrer suitabl dispers mixtur heterogen materi reagent hexan fail light petroleum bp oc chromatograph qualiti propanol ethanol analyt reagent qualiti propanol ethanol aqueou solut diethyl ether free peroxid aceton formic acid develop solvent mixtur hexan diethyl ether formic acid proport activ alumina chromatographi neutral grade brockmann silica powder binder suitabl qualiti thin layer chromatographi pancreat lipas suitabl qualiti note sodium hydroxid aqueou solut hydrochlor acid aqueou solut calcium chlorid cacl aqueou solut sodium cholat enzymat qualiti aqueou solut buffer solut aqueou solut tri hydroxymethylaminomethan bring ph addit hydrochlor acid check potentiomet phenolphthalein solut ethanol dichlorofluorescein solut ethanol render slightli alkalin addit drop sodium hydroxid solut ml control lipas activ neutral oil sodium hydroxid aqueou solut sodium cholat enzymat qualiti aqueou solut gum arab aqueou solut prepar sampl sampl acid determin annex ii purifi directli alumina sampl acid determin annex ii neutral alkali presenc solvent pass alumina neutral alkali presenc solvent separ funnel introduc crude oil add ml hexan ml propanol drop phenolphthalein solut amount sodium hydroxid solut free acid oil excess shake vigor minut add ml distil water shake leav settl separ remov bottom soap layer remov intermedi layer mucilag insolubl matter wash hexan solut neutral oil success ml portion propanol solut pink colour phenolphthalein disappear remov hexan distil vacuum rotari evapor dry oil oc vaccum current pure nitrogen hexan remov complet purif alumina prepar suspens activ alumina ml hexan pour stir chromatograph column alumina settl evenli solvent level fall mm absorb carefulli pour column solut oil ml hexan collect effluent column round bottom flask prepar chromatograph plate clean glass plate ethanol light petroleum aceton elimin trace fatti matter conic flask place silica powder add ml distil water stopper shake vigor minut transfer slurri immedi spreader coat clean plate layer mm thick dry plate air minut hour oven oc cool plate desicc room temperatur prepar plate commerc procedur hydrolysi pancreat lipas centrifug tube weigh prepar sampl sampl liquid oil proce directli add mg lipas ml buffer solut shake carefulli add ml sodium cholat solut ml calcium chlorid solut close tube ground stopper shake cautious avoid wet stopper put tube immedi thermostat maintain oc shake hand minut remov tube thermostat agit vigor mean electr shaker minut cool immedi run water add ml hydrochlor acid ml diethyl ether stopper mix vigor mean electr shaker stand remov organ layer mean syring centrifug separ monoglycerid thin layer chromatographi appli extract chromatograph plate microsyring cm bottom edg thin uniform line narrow set plate satur develop tank develop develop solvent oc cm top edg plate dry plate air temperatur tank sprai dichlorofluorescein solut identifi monoglycerid band rf ultraviolet light analysi monoglycerid ga liquid chromatographi remov band obtain spatula avoid remov compon remain base line transfer methyl flask treat collect silica directli method annex altern convert monoglycerid methyl ester examin ester ga chromatographi annex express result calcul fatti acid composit posit decim place note note note check activ lipas prepar oil emuls shake mixtur ml solut gum arab crush ic ml neutral oil suitabl agit beaker place ml emuls success ml sodium cholat solut ml distil water put beaker thermostat maintain oc note insert electrod ph meter spiral stirrer mean burett add dropwis sodium hydroxid solut ph reach add suffici aqueou suspens lipas sson ph meter ph start stop watch drip sodium hydroxid solut rate maintain ph read volum alkali solut consum minut record observ form graph time read abscissa ml alkali solut requir maintain ph constant ordin linear graph obtain lipas suspens mention thousand suspens water test suffici suspens ml alkali solut consum minut mg powder requir lipas unit defin amount enzym liber equival acid minut activ powder measur lipas unit mg formula note number sodium hydroxid solut consum minut calcul graph mass mg test portion powder note prepar lipas lipas satisfactori lipas activ commerci prepar laboratori chill kg fresh pig pancrea oc remov surround solid fat connect tissu tritur blender obtain pasti fluid stir past stirrer hour anhydr aceton centrifug extract residu thrice volum aceton mixtur aceton diethyl ether diethyl ether dry residu vacuo hour obtain stabl powder store refriger note case advis determin composit total fatti acid sampl comparison acid posit interpret figur obtain note temperatur hydrolysi set oc liquid oil set oc test sampl examin fat melt point oc annex viii determin composit trilinolein scope determin composit triglycerid oliv oil term thehir equival carbon number high perform liquid chromatographi present standard describ method separ quantit determin triglycerid composit veget oil term molecular weight degre unsatur function equival carbon number note field applic standard applic veget oil triglycerid long chain fatti acid method appliabl detect presenc small quantiti semi dry oil rich linol acid veget oil oleic acid predomin unsatur fatti acid oliv oil principl separ triglycerid equival carbon number high perform liquid chromatographi revers phase polar interpret chromatogram apparatu high perform liquid chromatograph allow thermostat control column temperatur inject unit ml deliveri detector differenti refractomet full scale sensit unit refract index column stainless steel tube mm length intern diamet mm pack mm diamet particl silica carbon form octadecylsilan note record integr reagent reagent analyt puriti elut solvent de gass recycl time effect separ chloroform aceton acetonitril elut solvent acetonitril aceton proport adjust obtain desir separ begin mixtur solubil solvent aceton aceton chloroform mixtur refer triglycerid commerci triglycerid tripalmitin triolein retent time plot accord equival carbon number altern refer chromatogram obtain soya oil note figur prepar sampl solut sampl analys prepar weigh sampl ml graduat flask make ml solubil solvent procedur set chromatograph system pump elut solvent rate ml mm purg entir system wait stabl base line obtain inject ml sampl prepar calcul express result intern standard method assum sum area peak triglycerid equal calcul rel percentag triglycerid formula triglycerid sum peak area triglycerid area peak sum peak area result decim place note elut order determin calcul equival carbon number defin relat ecn cn cn carbon number number doubl bond calcul precis take account origin doubl bond number doubl bond attribut oleic linol linolen acid equival carbon number calcul mean relat formula ecn cn coeffici calcul mean refer triglycerid condit method relat obtain close ecn cn note exampl lichrosorb merck rp art lichrospher equival merck ch art note refer triglycerid calcul resolut respect triolein rtm rtmolein reduc retent time rtm rt rtsolvent graph log number doubl bond enabl retent valu determin triglycerid fatti acid contain refer triglycerid figur note effici column permit clear separ peak trilinolein peak triglycerid adjac rt gt start graphic gt figur chromatogram sampl soya oil gt end graphic gt palmit acid st linolen acid palmit acid st stearic acid oleic acid linol acid ln linolen acid gt start graphic gt figur graph log number doubl bond gt end graphic gt annex ix spectrophotometr investig ultraviolet foreword spectrophotometr examin ultraviolet provid inform qualiti fat state preserv brought technolog process absorpt wavelength method due presenc conjug dien trien system absorpt express specif extinct cm extinct solut fat solvent thick cm convention refer extinct coeffici scope method describ procedur perform spectrophotometr examin fat ultraviolet principl method fat question dissolv requir solvent extinct solut determin wavelength refer pure solvent specif extinct calcul spectrophotomet read equip spectrophotomet measur extinct ultraviolet nm possibl read individu nanometr unit rectangular quartz cuvett cover optic length cm fill water suitabl solvent cuvett show differ extinct unit ml graduat flask chromatographi column length mm diamet mm discharg tube diamet approxim mm reagent spectrophotometr pure iso octan trimethylpentan refer distil water transmitt nm nm spectrophotometr pure cyclohexan refer distil water transmitt nm nm suitabl solvent capabl complet dissolv fat ethyl alcohol castor oil basic alumina column chromatographi prepar check appendix hexan chromatographi procedur sampl question perfectli homogen suspect impur oil liquid ambient temperatur filter paper temperatur approxim oc hard fat homogen filter temperatur oc melt point weigh accur approxim sampl prepar ml graduat flask make mark solvent homogen result solut perfectli clear opalesc turbid present filter quickli paper fill cuvett solut obtain measur extinct wavelength nm solvent refer extinct valu record lie rang measur repeat concentr dilut solut determin specif extinct requir passag alumina proce place basic alumina suspens hexan chromatographi column adsorb settl remov excess hexan approxim cm top alumina dissolv fat homogen filter ml hexan pour solut column collect eluat evapor solvent vacuum temperatur oc proce immedi fat obtain express result record specif extinct extinct coeffici wavelength calcul kl kl eë kë specif extinct wavelength eë extinct measur wavelength concentr solut ml thick cuvett cm result express decim place spectrophotometr analysi oliv oil accord offici method eec regul specifi determin specif extinct iso octan solut wavelength nm determin dk km km km dk km km km km specif extinct wavelength wavelength maximum absorpt nm appendix prepar alumina test activ preper alumina place alumina previous desicc furnac oc hour hermet seal contain add distil water ratio ml alumina immedi close contain shake repeatedli rest hour check activ alumina prepar chromatograph column alumina work paragraph pass mixtur consist virgin oliv oil specif extinct nm ground nut oil treat earth refin process specif extinct nm column passag column mixtur specif extinct nm alumina accept level dehydr increas appendix ii calibr spectrophotomet equip check interv month wavelength respons accuraci respons wavelength check mercuri vapour lamp mean suitabl filter order check respons photocel photomultipli proce weigh pure potassium chromat spectrophotometri dissolv potassium hydroxid solut ml graduat flask make mark precis ml solut obtain transfer ml graduat flask dilut mark potassium hydroxid solut measur extinct solut obtain nm potassium hydroxid solut refer extinct measur cm cuvett annex analysi ga chromatographi methyl ester fatti acid scope method gener guidanc applic ga chromatographi pack capillari column determin qualit quantit composit mixtur fatti acid methyl ester obtain accord method annex method applic polymer fatti acid reagent carrier ga inert ga nitrogen helium argon hydrogen dri oxygen content mg kg note hydrogen carrier ga capillari column doubl speed analysi hazard safeti devic auxiliari gase hydrogen puriti fere organ impur air oxygen free organ impur refer standard mixtur methyl ester pure fatti acid methyl ester fat composit prefer similar fatti matter analyz care prevent oxid polyunsatur fatti acid apparatu instruct relat usual equip ga chromatographi emploi pack capillari column flame ioniz detector apparatu give effici resolut suitabl ga chromatograph ga chromatograph compris element inject system inject system pack column deadspac case inject system capabl heat temperatur oc higher column capillari column case inject system special design column split type splitless column injector type note absenc fatti acid carbon atom move needl injector oven oven capabl heat column temperatur oc maintain desir temperatur oc pack column oc capillari column requir import fuse silica tube temperatur program heat recommend case fatti acid carbon atom pack column column construct materi inert substanc analyz glass stainless steel dimens length short column long chain fatti acid present analyz acid carbon atom recommend column length intern diamet mm note polyunsatur compon doubl bond present decompos stainless steel column note system pack twin column pack compris element support acid wash silan diatomac earth suitabl inert support narrow rang grain size mm rang limit mm averag grain size relat intern diamet length column stationari phase polyest type polar liquid diethylen glycol polysuccin butanediol polysuccin ethyleneglycol polyadip cyanosilicon liquid permit chromatograph separ requir claus stationari phase amount pack polar stationari phase separ condit column column disconnect detector gradual heat oven oc pass current inert ga freshli prepar column rate ml min hour temperatur hour oc capillari column tube made materi inert substanc analys glass fuse silica intern diamet mm intern surfac undergo treatment surfac prepar inactiv receiv stationari phase coat length mm suffici case stationari phase type polyglycol poli ethylen glycol polyest butanediol polysuccin polar polysiloxan cyanosilicon bond cross link column suitabl note risk polar polysiloxan give rise difficulti identif separ linolen acid acid coat thin mm assembl condit column observ normal precaut assembl capillari column arrang column oven support choic assembl joint leak tight posit end column injector detector reduct dead space place column flow carrier ga bar kpa column length mm intern diamet mm condit column temperatur program oven oc min ambient temperatur temperatur oc decomposur limit stationari phase maintain oven temperatur hour stabil baselin return oc work isotherm condit note suitabl pre condit column commerci detector prefer capabl heat temperatur column syring syring maximum capac ml graduat ml divis record record curv calcul composit mixtur analys electron record high precis compat apparatu requir record characterist rate respons prefer rate respons time record pen pass sudden introduct signal width paper cm minimum paper speed adjust valu cm min integr rapid accur calcul perform electron integr give linear respons adequ sensit correct deviat base line satisfactori procedur oper relat flame ioniz detector altern ga chromatograph emploi catharomet detector work principl thermal conduct oper condit modifi claus test condit select optimum oper condit pack column select test condit variabl account length diamet column natur amount stationari phase temperatur column carrier ga flow resolut requir size test portion select assembl detector electromet linear respons durat analysi gener valu tabl tabl lead desir result theoret plate metr column length methyl stearat elut minut apparatu injector temperatur oc detector temperatur equal higher column rule ratio flow rate hydrogen suppli flame ioniz detector carrier ga vari depend diamet column flow oxygen time hydrogen gt tabl gt gt tabl gt capillari column properti effici permeabl capillari column separ constitu durat analysi larg depend flow rate carrier ga column optim oper condit act paramet simpli headloss column wish improv separ make rapid analysi determin number theoret plate effici resolut figur carri analysi mixtur methyl stearat methyl oleat equival proport methyl ester cocoa butter choos temperatur column carrier ga flow maximum methyl stearat peak record minut solvent peak suffici quantiti mixtur methyl ester methyl stearat peak occupi quarter full scale calcul number theoret plate effici formula dr resolut formula dr retent distanc millimetr start chromatogram maximum peak methyl stearat width millimetr peak methyl stearat methyl oleat measur point intersect tangent point inflect curv base line distanc millimetr peak maxima methyl stearat methyl oleat gt start graphic gt figur chromatogram determin number theoret plate effici resolut gt end graphic gt oper condit select afford theoret plate metr column length methyl stearat resolut test portion syring ml solut methyl ester prepar annex inject column case ester solut prepar solut approxim mg ml heptan chromatograph qualiti inject ml solut analysi constitu present trace amount size test portion increas fold analysi gener oper condit defin work lower column temperatur determin fatti acid fewer carbon atom requir higher temperatur determin fatti acid carbon atom occas emploi temperatur program case sampl methyl ester fatti acid fewer carbon atom inject sampl oc oc butyr acid present immedi rais temperatur rate oc min optimum case procedur combin program heat continu elut constant temperatur compon elut instrument program heat fix temperatur oc recommend analysi carri fix phase polar verifi absenc mask peak case simultan presenc conjug prepar refer chromatogram refer graph analyz refer standard mixtur oper condit emploi sampl measur retent time retent distanc constitu fatti acid construct semi logarithm paper degre unsatur graph show logarithm retent time distanc function number carbon atom isotherm condit graph straight chain acid degre unsatur straight line line approxim parallel avoid condit mask peak exist resolut insuffici separ constitu express result qualit analysi identifi methyl ester peak sampl graph prepar interpol quantit analysi determin composit except case intern normal method assum compon sampl repres chromatogram total area peak repres constitu total elut equip includ integr figur obtain therefrom determin area peak multipli height peak width mid height account attenu record method calcul gener case calcul content compon express percentag mass methyl ester determin percentag repres area peak rel sum area peak formula óa ai óa ai area peak compon óa sum area peak give result decim place note gener case result calcul base rel area consid repres percentag mass case assumpt allow correct factor case presenc fatti acid fewer carbon atom acid secondari group thermal conduct detector highest degre accuraci requir correct factor convert percentag peak area mass percentag compon determin correct factor chromatogram deriv analysi refer mixtur methyl ester composit carri oper condit ident sampl refer mixtur percentag mass compon formula óm mi óm mi mass compon refer mixtur óm total mass compon refer mixtur chromatogram refer mixtur calcul percentag area area compon ai óa ai area peak compon óa sum area peak correct factor calcul ki mi óa ki mi óa ai óm commonli correct factor express rel kc rel factor kmi kc kmi ki kc sampl content compon express percentag mass methyl ester kmi ai kmi ai kmi ai give result decim place intern standard analys fatti acid quantifi acid carbon present alongsid acid carbon determin absolut amount fatti acid sampl intern standard fatti acid carbon frequent correct factor intern standard determin percentag mass compon express methyl ester formula ms kmi ai ms kmi ai km ai area peak compon area peak intern standard kmi correct factor compon rel kc km correct factor intern standard rel kc mass milligram test portion ms mass milligram intern standard give result decim place special case catharomet detector work principl thermal conduct ga chromatograph emploi detector work principl thermal conduct catharomet determin qualit quantit composit mixtur fatti acid methyl ester condit claus claus modifi shown tabl quantit analysi correct factor defin gt tabl gt test report test report method prepar methyl ester ga chromatograph analysi result obtain mention oper detail intern standard regard option detail incid influenc result test report includ inform complet identif sampl annex prepar methyl ester fatti acid accord titl ii annex vi regul eec method foreword choic process dictat acid composit acid fatti substanc examin ga chromatographi analysi carri specif seal phial process process dimethyl sulphat fatti substanc fatti acid fewer carbon atom methanol hydrochlor acid methyl sulphat process fatti substanc acid process sodium methyl dimethyl sulphat ga chromatographi measur tran isom methanol hexan sulphur acid process prepar methyl ester small quantiti fatti substanc separ thin layer chromatographi presenc unsaponifi discount provid exce methyl ester prepar fatti acid scope field applic descript process prepar methyl ester fatti substanc sodium methyl sodium methyl seal phial methanol hydrochlor acid seal phial dimethyl sulphat methanol hexan sulphur acid process principl fatti substanc subject analysi heat reflux methyl alcohol sodium methyl methyl ester obtain extract ethyl ether apparatu ml flask reflux condens soda lime tube fit top ground glass joint ml measur glass ml measur pipett mark ml ml separ funnel ml flask reagent anhydr methanol solut approxim sodium methyl methanol prepar dissolv metal sodium ml anhydr methanol ethyl ether sodium chlorid solut oc petroleum ether procedur place ml flask fatti substanc previous dri sodium sulphat filter add ml methanol fit condens boil reflux minut stop heat process remov condens add rapidli ml sodium methyl solut refit condens boil reflux hour methyl complet fatti substanc liquifi reagent mixtur perfectli clear room temperatur cool pour reagent mixtur ml separ funnel add ml ethyl ether ml water ml sodium chlorid solut shake layer separ transfer aqueou phase separ funnel extract ml ethyl ether add ml oc petroleum ether combin ether extract water separ elimin wash ether phase time ml water dry sodium sulphat filter paper collect filtrat ml flask evapor solvent ml complet process water bath current pure nitrogen process principl fatti substanc subject analysi treat sodium methyl methanol solut seal phial oc apparatu strong glass phial capac approxim ml height mm diamet mm ml measur pipett mark ml reagent solut approxim sodium methyl methanol prepar dissolv metal sodium ml anhydr methanol procedur place glass phial fatti substanc previous dri sodium sulphat filter add approxim ml sodium methyl solut heat seal phial immers phial hour oc oc shake time time esterif process complet content phial clear sediment glycerin residu reagent cool room temperatur open phial methyl ester requir treatment ga chromatographi apparatu process principl fatti substanc subject analysi treat methanol hydrochlor acid seal phial oc apparatu strong glass phial capac approxim ml height mm diamet mm ml calibr pipett reagent solut hydrochlor acid methanol prepar gaseou hydrochlor acid anhydr methanol note hexan ga chromatographi procedur place glass phial fatti substanc previous dri sodium sulphat filter ml hydrochlor acid methanol solut heat seal phial immers phial oc minut cool phial run water open add ml distil water ml hexan centrifug remov hexan phase readi process principl fatti substanc subject analysi saponifi methyl alcohol solut potassium hydroxid treat dimethyl sulphat hydrochlor acid ad methyl ester form automat separ pure methyl ester obtain subsequ treatment alumina apparatu strong test tube capac approxim ml ground glass stopper safeti clip reflux condens ground glass attach glass filter sinter disc size mm diamet glass test tube capac approxim ml conic base ml ml syring reagent potassium hydroxid solut methyl alcohol ga chromatographi green bromocresol indic solut methyl alcohol dimethyl sulphat oc concentr hydrochlor acid dilut equal part methyl alcohol ga chromatographi brockmann aluminium oxid adsorpt chromatographi procedur place ml test tube ml fatti substanc previous dri sodium sulphat filter add ml potassium hydroxid solut quartz granul control boil attach reflux condens heat low flame minut shake saponif complet solut clear final cool run water remov condens add drop indic syring ml dimethyl sulphat slowli seal test tube hermet shake minut immers bottom test tube boil water bath frequent interv reaction complet indic blue yellow final cool test tube run water open add ml hydrochlor acid methanol solut shake second lai test tube angl tap lightli methyl ester rise surfac form oili mass note remov methyl ester syring place test tube conic base add volum alumina equal approxim volum methyl ester shake filter filter paper note methyl ester separ spontan add ml water test tube shake process principl fatti substanc subject analysi heat reflux methanol hexan sulphur acid methyl ester obtain extract petroleum ether apparatu test tube capac approxim ml fit air reflux condens approxim length ground glass joint ml calibr pipett ml separ funnel ml ml measur glass ml test tube conic base reagent methyl reagent anhydr methanol hexan concentr sulphur acid ratio oc petroleum ether anhydr sodium sulphat procedur place matter plate ml test tube add ml methyl reagent fit reflux condens heat minut boil water bath note transfer quantit mixtur ml separ funnel aid ml distil water ml petroleum ether shake vigor phase separ remov aqueou phase wash ether layer ml distil water add separ funnel small quantiti anhydr sodium sulphat shake settl minut filter collect filtrat ml test tube conic base evapor solvent water bath current nitrogen note small amount gaseou hydrochlor acid easili prepar laboratori simpl displac commerci solut drip concentr sulphur acid liber ga easili dri bubbl conc sulphur acid hydrochlor acid rapidli absorb methanol advis usual precaut dissolv introduc ga small invert funnel rim touch surfac liquid larg quantiti methanol hydrochlor acid solut prepar advanc perfectli glass stopper bottl store dark note control boil point insert glass rod test tube limit temperatur water bath oc annex xi determin volatil halogen solvent content oliv oil method analysi ga chromatographi head space techniqu equip ga chromatographi apparatu fit electron captur detector ecd head space apparatu ga chromatographi column glass long mm diamet stationari phase ov equival impregn calcin diatomac earth acid wash silanis particl size mesh carrier auxiliari ga nitrogen ga chromatographi suitabl detect electron captur glass flask ml teflon coat aluminium stopper fitment entri syring hermet seal clamp ga syring ml reagent standard halogen solvent degre puriti suitabl ga chromatographi procedur weigh oil glass flask reus hermet seal place thermostat oc hour syring carefulli remov ml head space inject column ga chromatographi apparatu regul injector temperatur oc column temperatur oc detector temperatur oc temperatur provid result remain equival refer solut prepar standard solut refin oliv oil trace solvent concentr rang ppm mg kg presum content sampl halogen solvent dilut pentan quantit assess correl surfac elev peak sampl standard solut concentr presum closest deviat greater analysi repeat comparison standard solut deviat content determin basi averag elementari inject express result ppm mg kg detect limit method mg kg annex xii organolept assess virgin oliv oil scope purpos method determin criteria need assess flavour characterist virgin oliv oil develop methodolog requir field applic method applic organolept assess classif virgin oliv oil direct consumpt confin grade virgin oil numer scale relat percept flavour stimuli judgement group select taster work panel gener basic vocabulari sensori analysi chapter entitl sensori analysi gener basic vocabulari specif vocabulari oliv oil almond flavour form typic fresh almond peculiar dri sound almond confus incipi rancid distinct tast perceiv aftertast oil remain contact tongu palat sweet oil flat odour appl flavour oliv oil reminisc fruit atrojado fusti characterist flavour oil obtain oliv store pile undergon advanc stage ferment bitter characterist tast oil obtain green oliv oliv turn colour pleasant depend intens brine flavour oil extract oliv preserv salin solut cucumb flavour produc oil hermet pack long tin contain attribut format nonadien earthi characterist flavour oil obtain oliv collect earth mud wash flavour accompani musti humid flavour esparto characterist flavour oil obtain oliv press esparto mat flavour differ depend mat made green esparto dri esparto flat smooth flavour oliv oil organolept characterist weak ow loss aromat compon fruiti flavour reminisc odour tast sound fresh fruit pick optimum stage ripe grass characterist flavour oil reminisc recent mown grass greasi odour oliv oil extract plant residu petroleum greas miner oil properli remov machineri green leav bitter flavour oil obtain excess green oliv oliv crush leav twig grubbi characterist flavour oil obtain oliv heavili attack grub oliv fly dacu olea harsh characterist sensat oil tast produc mouthfeel reaction astring hai characterist flavour oil reminisc dri grass heat burnt characterist flavour oil caus excess prolong heat process past thermal mix unsuit condit metal flavour reminisc metal characterist oil prolong contact unsuit condit foodstuff metal surfac crush mix press storag muddi sediment characterist flavour oil recov decant sediment vat underground tank musti humid characterist flavour oil obtain fruit larg number fungi yeast develop result store pile humid condit dai characterist flavour oil long storag contain oil pack excess long period pomac characterist flavour reminisc flavour oliv pomac press mat characterist flavour oil obtain oliv press dirti press mat ferment residu left rancid characterist flavour common oil fat undergon process auto oxid caus prolong contact air flavour unpleas correct ripe fruiti flavour oliv oil obtain ripe fruit gener flat odour sweet tast rough characterist percept oil tast produc thick pasti mouthfeel sensat soapi flavour produc olfactori gustatori sensat reminisc produc green soap sweet pleasant tast sugari found oil bitter stringent pungent attribut predomin veget water characterist flavour acquir oil result poor decant prolong contact veget water winei vinegari characterist flavour oil reminisc wine vinegar due format acet acid ethyl acet ethanol larger amount usual aroma oliv oil glass oil tast chapter entitl glass oil tast test room chapter entitl guid instal test room apparatu apparatu requir taster perform task properli suppli booth easi reach glass standard sampl mark legend consist randomli pick figur figur letter mark made indel odourless pencil watch glass ident mark cover glass grade sheet figur instruct pencil pen small trai slice appl glass water ambient temperatur methodolog section stipul prior knowledg requir perform sensori analysi virgin oliv oil attempt standard conduct procedur taster particip test awar gener specif recommend oliv oil tast duti panel organ supervisor panel panel organ suitabl train knowledg person expert kind oil work kei figur panel respons organ run summon taster suffici advanc clarifi doubt perform test refrain suggest opinion sampl respons inventori apparatu ensur properli clean prepar code sampl present taster accord experiment design assembl statist process data obtain result obtain minimum effort work panel supervisor call sensori skill meticul prepar test rigor arrang skill patienc plan execut test duti panel supervisor stimul moral panel member encourag interest curios competit spirit ensur opinion prevent leader assert criteria taster respons train select monitor taster order ascertain keep adequ level aptitud test condit sampl size glass ml oil test temperatur oil sampl test glass oc oc temperatur chosen easili observ organolept differ normal temperatur oil condiment factor weigh favour higher lower temperatur aromat compon scarc volatil volatil compon produc peculiar heat oil test time morn time test oil prove optimum percept period tast smell dai meal preced period olfactori gustatori sensit increas percept decreas criterion extrem hunger distract taster decreas discriminatori capac prefer accept criteria taster peopl act taster organolept test carri edibl oliv oil train select accord skill distinuish similar sampl born mind accuraci improv train section taster requir test wise extra taster reserv cover absenc gener recommend candid taster recommend appli conduct candid taster work call panel supervisor particip organolept test taster attend time set observ smoke minut time set test perfum cosmet soap small linger time test unperfum slightli perfum soap wash hand rins dry elimin smell fast hour tast carri feel physic unwel sens smell tast affect psycholog effect prevent concentr work taster inform panel supervisor view withdrawn test decis bear mind deviat valu rest panel compli taster place booth allot orderli quiet manner seat check correct apparatu properli arrang ensur legend glass match legend watch glass carefulli read instruct grade sheet begin examin sampl absolut task perform doubt aris discuss difficulti encount privat panel supervisor taster pick glass keep cover watch glass bend gentli rotat glass fulli posit wet insid stage complet remov watch glass smell sampl take slow deep breath form criterion oil assess smell exce second conclus reach time short rest olfactori test perform taster judg flavour olfactori gustatori tactil sensat small sip approxim ml oil import distribut oil mouth caviti front part mouth tongu side back part palat support fact percept primari tast sweet salti acid bitter vari intens depend area tongu palat stress essenti suffici amount oil spread slowli back tongu throat taster concentr order bitter pungent stimuli stimuli escap notic oil bitter stimulu obscur pungent stimulu take short success breath draw air mouth enabl taster spread sampl extens mouth perceiv volatil aromat compon back nose tactil sensat consider fluiditi sticki sharp sting note detect requir test intens quantifi organolept assess virgin oliv oil sampl evalu session avoid contrast effect produc immedi tast sampl success tast produc fatigu loss sensit import product elimin remain oil preced tast mouth small slice appl recommend chew spat spittoon rins mouth water ambient temperatur minut laps end tast start screen candid stage complet panel organ person interview candid familiar person surround environ physio psycholog condit met rigor theoret normal person particip factor sex ag specif habit smoke supersed nowadai health person interest time work interview panel organ explain characterist task candid approxim time obtain inform candid allow assess interest motiv real time questionnair refer questionnair answer question involv work topic work contribut qualiti improv foodstuff domest intern front awar fact taht tast oil call prepar compar olfactori gustatori skill colleagu time wise independ organ daili work depend superior absent usual job half hour occas sucess number dai allow make time lost job due particip sensori analys remuner work organis inform screen candid reject show interest kind work readili incap express determin threshold group characterist attribut carefulli choos oil consid repres attribut atrojado fusti winei rancid bitter great clear intens aliquot part oil prepar sampl concentr differ ratio compris success dilut support differ detect glass support dilut pair glass support complet seri glass higher concentr total reach prepar suffici amount sampl prepar concentr complet seri attribut candid establish threshold candid attribut give glass ml prepar concentr glass ml support sole perform test candid repeat test remain concentr attribut consider note number correct answer obtain concentr taster give figur percentag number test perform describ gain organolept assess foodstuff oliv oil plot abscissa ascend order concentr test ordin correct identif concentr figur practic instruct detect threshold determin extrapol ordin point repres correct apprais curv abscissa threshold concentr initi oil depend intens attribut present similar group candid panel link habit tendenti prefer point refer common normal human group homogen panel olfactori gustatori sensit basi threshold concentr obtain group proce prepar seri increas decreas concentr threshold concentr hold place scale natur concentr dilut result difficult detect presenc oil possess select attribut take concentr basi remain sampl prepar accord formula constant dilut factor equal expon vari assum threshold obtain rancid oil basi seri sampl concentr gt tabl gt procedur repeat remain attribut basi respect threshold calcul scale similar aromat intens stimulu obtain laboratori defect initi oil percept intens select taster intens rate method select procedur time candid requir panel peopl sensit power discrimin pick advis product subsequ analys oliv oil gt start graphic gt figur gt end graphic gt select method overlook effect procedur adopt econom quantiti oil number sampl time spent select effectivencess select procedur li choic optimum level depend variabl cost determin number test proport potenti suitabl candid chanc elimin screen proport candid chanc select process unsuit materi point select procedur chosen intens rate test astm american societi test materi stp special technic public page modifi decreas number sampl seri broaden rang stimuli view increas number olfactori gustatori note select base adapt common defect perceiv oliv oil vari concentr ratio seri statist process result apparatu requir ml bottl glass flask dark colour tast glass graduat ml test tube product requir merck paraffin refer dab usp xx oili support tast odour recent refin oliv oil similar oil oil atrojado fusti winei rancid bitter procedur prepar dilut select stage begin candid accord methodolog stimulu prepar seri tast glass mark code seri candid pour ml concentr prepar formula respect tast glass tast glass fill left cover watch glass tast room temperatur oc hour start test homogen temperatur ambient temperatur organ arrang tast glass seri row descend order concentr step candid test accord instruct instruct candid tast glass line front candid dilut atrojado fusti winei rancid bitter stimuli distinguish factor content tast glass intens odour glass intens odour left hand side rest glass descend order intens tast glass weak odour imposs detect proce familiar odour tast glass seri begin hand side retain intens odour overtir feel scale concentr odour leav room organ remov tast glass seri place level hand side move fill space left return room carri test test involv tast glass withdrawn seri put back exact place smell compar wish bear mind replac correctli smell stronger sampl weaker left test repeat glass candid issu form addit instruct make test collect repli easier select candid test attribut glass belong posit date obtain result panel organ record data candid manner facilit arrang gt tabl gt statist grade procedur select case tast glass replac exact posit candid statist calcul purpos correspond posit order seri attribut gt tabl gt number posit glass order seri vari statist calul test ey probabl glass randomli put back exact posit order make extrem difficult inform pass candid panel organ ensur mean contact candid legend candid candid find posit glass withdrawn thouch candid present glass earlier order hand candid vari candid grade depend perform manner ei ei ei glass concentr attribut attribut atrojado fusti winei rancid bitter arrang descend order intens eik glass pick kí posit alloc candid replac seri valu km number inclus real place number glass chosen alloc candid maximum permit deviat set case equal kí gt candid automat reject contrari kí theoret candid accept test put stimulu back exact posit case grade award candid assess set stimulu concentr instanc atrojado fusti seri fy equal squar differ exact number glass order seri posit candid replac ph fy kí oper conduct candid stimuli concentr attribut partial grade attribut fy zfy pfyh pfyj pfyl pfym exampl facilit comprehens oper assum answer candid stimuli withdrawn seri attribut gt tabl gt assum candid rearrang glass attribut gt tabl gt candid reject obtain grade zi candid final grade seal accept reject select taster depend respons attribut consider pat pam pam pam final zat zam phfy pjfy plfy pmfy zfy phw pjw plw pmw zw phrd pjrd plrd pmrd zrd phbt pjbt plbt pmbt zbt final zfy zbt fy atrojado fusti winei rancid bt bitter question determin maximum candid consid good level percept olfactori retent intellectu organ give correct answer stimuli consid neg mean candid recogn correctli quantifi intens present attribut valu candid recogn scale area select intens pick area unabl locat exact posit abil discrimin scale intens present stimuli satisfactori critic zc determin candid randomli replac glass insid area recogn probabl final grade zc suffici small quantiti set word ensur probabl procedur select taster panel show suffici discriminatori power intens stimuli select process set case zc obtain probabl distribut variabl turn depend probabl distribut variabl kí relev statist calcul zc grade candid obtain candid grade li elimin grade candid gt tabl gt candid consid respect valu candid retain candid reject candid grade elimin remaind classifi valu candid chosen train chief aim train stage familiar taster multipl olfactori gustatori tactil variant found virgin oliv oil familiar taster specif sensori methodolog heighten individu skill recogn identifi quantifi sensori attribut improv sensit memori attribut consid end result precis consist assess train stage entail number session depend possibl open panel studi individu analys oil taster discuss difficulti encount panel organ comment mark unifi criteria opinion standard reach train set number session assess term percentag increas exact repli discriminatori trial analys varianc averag individu mark panel test scale implement practic util train period discuss great length present consid effect essenti exact accur sensori data obtain perform check panel veteran taster carri tast regular continu basi involv sensori test requir great effort part decis great technolog commerci import depend larg major case judgement reason select train taster perform check ensur result precis panel set routin trial regularli check perform suitabl interv procedur organolept assess virgin oliv oil condit standard met facil panel select taster smell tast sampl oil analysi contain tast glass analys olfactori gustatori tactil kinaesthet percept aid sheet shown figur record note present degre intens step grade qualiti oil sheet figur flavour descript qualiti grade characterist sensori percept frequent found oliv oil describ flavour list left hand side page taster encount stimuli correspond descriptor list note descriptor defin accur percept stimuli assess proport intens cross box accord criteria bare percept slight averag great extrem hand side sheet scale point shown except qualiti worst taster give singl grade characterist oil examin grade consist good point defect oil note left hand side sheet column defect grade tabl divid section classif oil base primarili total absenc presenc defect flavour intens flavour grade scale point nuanc aspect account reach conclus decis total qualiti grade column head characterist final grade panel supervisor collect form fill taster check sensori attribut intens attribut perceiv record profil sheet agre assess oil enter grade sheet notic differ supervis taster check grade sheet taster repeat test final panel supervisor make tabl grade group calcul arithmet degre error case revis analys group repeat test view obtain assess triplic sampl final grade decim figur grade intens rate bitter pungenc greater oil mark record bitter pungent express result basi averag grade panel supervisor determin categori sampl classifi accord limit laid annex analysi report show categori note sampl seal refriger analys return refriger analysi test triplic gt start graphic gt gt end graphic gt sensori analysi gener basic vocabulari scope purpos standard assembl gener term sensori analysi give definit vocabulari gener terminolog sensori analysi noun examin organolept attribut product sens organ percept noun sensori awar extern object event organolept adject attribut describ attribut product percept sens organ expert noun regard examin organolept attribut taster special sensori analysi specif product basic understand prepar product market prefer taster noun perspicaci sensit train person select evalu organolept attribut food sens organ panel group assessor special select train assembl perform sensori analysi product control condit sensat noun subject phenomenon result stimul sensori system phenomenon subject discrimin object defin sens organ involv depend natur kind stimulu intens sensit noun abil perceiv quantit qualit stimulu intens small differ stimuli mean sens organ tast noun oper involv perceiv analys judg organolept attribut product olfactori gustatori tactil kinaesthet attribut food product accept noun act individu popul favour accept product harmoni noun attribut product rise pleasant sensat sensat produc percept product compon olfactori gustatori tactil kinaesthet stimuli present suitabl concentr ratio accept noun state product favour receiv individu popul term organolept attribut discrimin noun act qualit quantit differenti stimuli compens noun result interact combin stimuli perceiv intens act aspect noun combin organolept attribut perceiv visual size shape colour conform turbid clean fluid foam effervesc term prefer term appear attribut noun percept characterist physiolog term stimulu noun physic chemic agent specif produc respons extern intern sensori receptor tast noun sens tast sens receptor locat mouth tongu activ compound solut gustatori adject describ attribut product stimul gustatori apparatu awaken sensat pertain primari tast sweet salti acid bitter receptor noun specif structur sens organ excit capabl receiv stimulu convert nervou discharg note receptor classifi term type energi stimulu light heat sound olfact noun function olfactori apparatu perceiv discrimin molecul reach ga form extern environ directli indirectli nose intens noun magnitud energi attribut measur term quantit scale valu threshold adapt noun temporari modif sensit perceiv sensori stimuli due contin repeat exposur stimulu similar inhibit noun lack respons sens organ part thereof subject action suitabl stimulu intens threshold respons noun action sensori cell respond action stimuli relat sens organ bodi noun tactil sensat perceiv mouth degre densiti viscos consist compact product fragranc noun fresh pleasant delici odour smell verb activ sens appli smell describ act perceiv odour object adject describ true verifi represent object minim human factor instanc prefer habit inclin describ techniqu mean sensori instrument method minim induc error note term instrument synonym advis subject adject describ produc percept influenc stimulu think feel kinaesthesi sensat result pressur sampl produc movement oral caviti finger press chees finger threshold noun absolut threshold minimum sensori stimulu rise appear sensat stimulu threshold detect threshold identif sensat recognit threshold differ threshold minimum sensori stimulu rise percept differ intens sensat termin threshold maximum stimulu increas intens perceiv prefer threshold minimum quantit stimulu critic supra threshold stimulu attract reject respons appear relat neutral stimulu choic sugar solut water note distinct drawn absolut prefer threshold differenti prefer threshold threshold adject absolut threshold supra threshold adject absolut threshold sensori fatigu specif form sensori adapt decreas sensit occur compens noun result interact combin stimuli perceiv intens act synerg adject joint effect action substanc intens organolept attribut result combin excess sum intens attribut separ contrast effect increas respons differ simultan consecut stimuli opposit converg effect converg effect decreas respons differ simultan consecut stimuli opposit contrast effect terminolog relat organolept attribut acid adject describ primari tast produc dilut aqueou solut acid substanc citric acid lactic acid tartar acid describ attribut pure substanc mixtur produc tast noun acid sour adject describ olfactori gustatori sensat acid gener produc ferment predomin foodstuff produc sensat factor contribut sensat relat ferment lactic acet ferment food product bitter adject describ primari tast produc dilut aqueou solut substanc quinin caffein alkaloid describ attribut pure substanc mixtur produc tast noun bitter salti adject characterist sensat perceiv sens tast typic produc sodium chlorid solut describ attribut pure substanc mixtur produc tast noun salti sweet adject describ primari tast produc aqueou solut substanc sucros describ attribut pure substanc mixtur produc tast noun sweet astring adject describ complex sensat produc mouth dilut aqueou solut product tannin kaki tannin sloe tannin describ attribut pure substanc mixtur produc sensat noun astring flavour noun flavour mean combin olfactori gustatori tactil kinaesthet sensat enabl assessor identifi establish multi level favour unfavour criterion tast noun sensat perceiv gustatori papilla stimul solubl substanc attribut specif sensat produc substanc primari tast noun distinct tast held sweet salti acid bitter odour noun combin sensat perceiv olfactori organ snif volatil substanc attribut specif sensat produc substanc aroma noun pleasant sensat perceiv indirectli olfactori organ tast food perfumeri special languag term appli sensat perceiv directli nose tast residu tast noun combin sensat perceiv stimulu disappear mouth differ sensat perceiv aromat adject describ attribut pure substanc mixtur tast produc sensat aroma describ product examin directli nose produc sensat fragranc fresh textur noun characterist solid rheolog state product combin stimul mechan receptor tast locat mouth note term refer sole object attribut sensat produc design gener term consist fibrous greasi mouthwash action food present mouth contact sensit area mouth buccal sensat produc perceiv note vocabulari enlarg consult iso standard part public magnen entitl le cahier techniqu du centr nation de coordin de etud recherch sur la nutrit aliment glass oil tast scope purpos standard describ characterist glass intend organolept analysi edibl oil odour tast flavour addit describ adapt heat unit need reach maintain temperatur analysi descript glass draw figur attempt establish optimum characterist desir piec apparatu kind maximum steadi prevent glass tilt oil spill base easili fit indent heat unit bottom glass evenli heat shape broadest base volatil compon oil readili releas narrow mouth compon easili concentr ensur perceiv identifi nose made dark colour glass prevent taster perceiv colour oil elimin prejudic imped format bias tendenc dimens glass sketch figur dimens total capac ml ml total height mm mm diamet mouth mm mm diamet glass widest mm mm base diamet mm mm thick glass side mm mm thick glass base mm mm glass equip watch glass diamet mm larger mouth glass watch glass cover prevent loss aroma entri dust manufactur characterist glass made resist glass dark colour colour content discern free scratch bubbl rim smooth flang glass anneal stand temperatur undergo test instruct glass clean unperfum soap deterg rins repeatedli clean agent total elimin final rins distil water glass left drain dri desicc stove concentr acid chromic acid mixtur glass stove requir cupboard protect contamin extran odour glass smell ensur extran odour present test prepar care record legend glass oil test organ person legend oil relat devic heat sampl sampl organolept examin set temperatur case oil purpos heat devic figur instal booth taster reach compris aluminium block immers thermostat control water bath constant temperatur block seri indent fit bottom glass temperatur differ heat devic oil contain glass insert indent block gt start graphic gt figur tast glass gt end graphic gt gt start graphic gt figur devic heat sampl dimens millimetr gt end graphic gt guid instal test room introduct test room design provid panel particip sensori test suitabl comfort standard environ facilit work help improv repeat reproduc result scope purpos standard basic condit met instal test room gener specif instal premis larg meet specif pleasant suitabl light neutral style purpos sooth plain light colour recommend wall relax atmospher creat premis easili clean separ sourc nois prefer soundproof free extran odour purpos fit effect ventil devic fluctuat ambient temperatur warrant test room equip air condit atmospher close dimens dimens premis depend possibl laboratori compani gener suffici spaciou permit instal booth area prepar sampl obviou larger area set instal auxiliari area provid instanc clean apparatu arrang culinari prepar assembl open panel light gener light sunlight lamp instanc strip light uniform control diffus temperatur hygrometr condit premis constantli pleasant temperatur agreeabl hygrometr condit special circumst temperatur hygrometr condit rel humid recommend descript booth gener characterist sensori analysi booth alongsid premis ident separ partit suffici high wide isol taster seat booth made materi easili clean look instanc wood vitrifi plywood lamin panel paint complet odour free dry seat provid booth comfort adjust height devic booth provid individu light direct intens adjust highli recommend booth equip button connect light enabl taster make attend finish test requir sampl miss piec apparatu notic irregular wish inform distract taster dimens booth suffici larg comfort gener dimens width sink sink colour scheme room light affect result sensori analysi length tabl excess partit height partit minimum tabl height tabl arrang tabl surfac easili clean part surfac sink provid run drink water practic space trai spittoon similar piec equip sampl test constant temperatur ambient temperatur advis suitabl devic purpos bain mari hot plate shelf set height approxim metr floor place accessori glass small apparatu arrang booth test room permit worthwhil instal devic facilit present sampl form slide hatch figur revolv vertic devic figur suitabl glass cup tall contain horizont open hatch contain sampl small figur simpli question ensur open larg trai glass sampl pass figur test room addit premis addit premis suffici space advis provid separ premis prepar sampl culinari arrang glass apparatu hold discuss prior test premis clean smell nois convers premis disturb work assessor test room note ideal condit instal sole sensori analys test perform premis meet minimum condit light temperatur nois odour set mobil booth made fold element isol taster gt start graphic gt arrang booth figur gt end graphic gt gt start graphic gt revolv devic present sampl figur gt end graphic gt gt start graphic gt hatch present sampl figur gt end graphic gt gt start graphic gt sensori analysi laboratori figur test room tast booth room clean apparatu prepar sampl open panel offic wait room refriger oven dishwash pi sink ar cupboard mr trollei df distribut form mc round tabl work surfac quot end graphic gt panel organ press candid proce lose sensit olfactori fatigu refrain find extrem intens unpleas attribut odour record grade sheet except occurr annex xiii proof refin place neutral decolor oliv oil laboratori neutral oil apparatu beaker ml tall laboratori centrifug ml tube beaker ml round bottom flask ml separ funnel litr reagent aqueou solut sodium hydroxid ethyl alcohol solut phenolphtalein pure hexan ar pure propan ol ar procedur oil free fatti acid content express oleic acid place crude oil tall ml beaker heat water bath add quantiti solut sodium hydroxid free acid oil excess stir gentli time continu stir minut keep temperatur transfer mixtur ml centrifug tube separ soapi past centrifug pour decant oil ml beaker wash ml boil distil water remov water mean siphon repeat wash trace residu soap remov disappear pink colour phenolphtalein centrifug oil elimin small quantiti residu water oil free fatti acid content express oleic acid exceed litr separ funnel place crude oil ml hexan ml propan ol quantiti solut sodium hydroxid free acid oil excess stir vigor minut add ml distil water stir stand separ layer lower layer soap drain layer oili top aqueou underneath intermediari layer form made mucilag insolubl substanc elimin decolor neutral oil apparatu round bottom flask ml ground glass neck insert thermomet graduat degre allow read mechan stirrer oper revolut minut equip oper vacuum vacuum pump connect vacuum pump manomet capabl give residu pressur millibar procedur weigh neutral oil neck flask insert thermomet stirrer connect vacuum pump heat stir time maintain temperatur continu stir oil analys free water minut break vacuum add activ earth establish vacuum residu pressur millibar obtain maintain temperatur stir minut revolut minut filter hot thermostat oven annex xiv addit note chapter combin nomenclatur note purpos cn code oliv oil quot mean oil deriv sole treatment oliv exclud esterifi oliv oil mixtur oliv oil oil presenc esterifi oliv oil oil ascertain method set annex vii ix xii analyt characterist sterol acid composit oliv oil cn code set tabl gt tabl gt note virgin oliv oil quot mean oil deriv sole oliv mechan physic mean condit thermal condit lead deterior oil undergon treatment wash decant centrifug filtrat exclud oil extract oliv solvent defin section ii ii purpos subhead virgin lampant oliv oil quot acid mean oliv oil aliphat alcohol content exceed mg kg erythrodiol uvaol content exceed content satur fatti acid posit triglycerid exceed characterist peroxid number exceed meq kg content volatil halogen solvent exceed mg kg exceed mg kg solvent extinct coeffici higher treatment oil activ alumina higher oil free fatti acid content express oleic acid passag activ alumina accord method set annex xv extinct coeffici higher neutral decolour laboratori characterist extinct coeffici higher extinct coeffici variat dk nm region higher higher organolept characterist includ detect defect exceed limit accept panel test score lower ii purpos subhead virgin oil quot mean oliv oil characterist acid content express oleic acid exceed peroxid number exceed meq kg aliphat alcohol content exceed mg kg content volatil halogen solvent exceed mg kg exceed mg kg solvent extinct coeffici higher treatment oil activ alumina higher extinct coeffici variat dk nm region higher organolept characterist includ detect defect limit accept panel test score higher erythrodiol uvaol content exceed content satur fatti acid posit triglycerid exceed note subhead cover oliv oil obtain treatment oliv oil fall subhead blend virgin oliv oil characterist acid content express oleic acid exceed aliphat alcohol content exceed mg kg extinct coeffici higher higher treatment sampl activ alumina higher extinct coeffici variat dk nm region higher higher erythrodiol uvaol content exceed content satur fatti acid posit exceed note purpos subhead crude oil quot mean oil oliv residu oil characterist acid content express oleic acid greater erythrodiol uvaol content exceed content satur fatti acid posit triglycerid exceed note oil subhead includ oil obtain treatment oil fall subhead blend virgin oliv oil characterist oil refer point ii provid content satur fatti acid posit triglycerid exceed km mean extinct coeffici wavelength peak absorpt curv nm region km km mean extinct coeffici wavelength nm lower higher km wavelength note subhead cover residu result treatment fatti substanc oil iodin index determin accord method laid annex xvi lower higher residu result treatment fatti substanc oil iodin index lower higher peak area repres retent volum sitosterol determin accord annex regul mention addit note total sterol peak area note analyt method determin characterist product refer laid annex regul eec aek km km km exce test carri passag alumina exce annex xv oil content oliv residu apparatu suitabl extract apparatu fit ml round bottom flask electr heat bath sand bath water bath hotplat analyt balanc oven regul maximum electr heat oven fit thermostat devic regul swept stream air oper reduc pressur mechan mill easi clean oliv residu ground rise temperatur appreci alter content moistur volatil matter substanc extract hexan extract thimbl cotton wool filter paper substanc extract hexan remov dessic siev mm diamet apertur small particl previous dri pumic stone reagent normal hexan technic grade leav residu ml complet evapor procedur prepar test sampl mechan mill previous properli clean grind laboratori sampl order reduc particl pass complet siev twentieth sampl complet process clean mill discard ground materi grind remaind collect mix carefulli analyz delai test portion grind oper complet weigh sampl nearest test prepar extract thimbl place test portion thimbl plug cotton wool filter paper envelop test portion peliminari dry oliv residu moist moistur volatil matter content carri preliminari dry place load thimbl filter paper oven heat time order reduc moistur volatil matter content prepar round bottom flask weigh nearest mg flask particl pumic stone previous dri stove cool dessic hour initi extract extract apparatu insert thimbl filter paper test portion pour flask requisit quantiti hexan fit flask extract apparatu place electr heat bath adjust rate heat reflux rate drop moder violent boil hour extract cool remov thimbl extract apparatu place stream air order drive impregn solvent extract tip content thimbl micro grinder grind fine return ground mixtur thimbl loss place back extract apparatu continu extract hour round bottom flask initi extract result solut extract flask clear filter filter paper wash origin flask filter paper time hexan collect filtrat wash solvent round bottom flask dri tare nearest mg remov solvent weigh extract remov greater part solvent distil electr heat bath remov trace solvent heat flask oven minut assist elimin process blow air prefer inert ga interv reduc pressur leav flask dessic cool hour weigh nearest mg heat minut condit cool dessic reweigh differ weigh exce mg heat period minut cool weigh weight differ mg note weight flask carri duplic determin test sampl express result method calcul formula extract express percentag mass product receiv equal percentag mass extract product receiv mass gram test portion mass gram extract dry result arithmet duplic determin provid repeat condit satisfi express result decim place extract express dry matter basi formula oil percentag extract dry basi oil percentag extract dry basi percentag extract mean product receìv moistur volatil matter content repeat differ duplic determin carri simultan rapid sucess analyst exce hexan extract sampl condit satisfi repeat analysi test portion case differ exce result arithmet determin annex xvi determin iodin scope intern standard specifi method determin iodin anim veget fat oil refer fat definit purpos intern standard definit appli iodin mass iodin absorb sampl oper condit intern standard iodin express gram iodin sampl principl dissolut test portion solvent addit wij reagent time addit potassium iodid solut water titrat liber iodin sodium thiosulf solut reagent reagent recogn analyt grade water compli requir iso grade potassium iodid solut iodat free iodin starch solut mix solubl starch ml water add mixtur ml boil water boil minut cool sodium thiosulf standard volumetr solut na mol standard dai solvent prepar mix equal volum cyclohexan acet acid wij reagent iodin monochlorid acet acid commerci wij reagent apparatu usual laboratori apparatu glass weigh scoop suitabl test portion insert flask conic flask ml capac fit ground glass stopper complet dry prepar test sampl homogen sampl dri sodium sulphat filter procedur test portion mass test portion vari expect iodin shown tabl gt tabl gt weigh test portion nearest mg glass weigh scoop determin place test portion ml flask add ml solvent dissolv fat add ml wij reagent insert stopper swirl content place flask dark mouth pipett wij reagent similarili prepar blank solvent reagent omit test portion sampl iodin valv leav flask dark hour iodin polymer product product oxid consider extent leav hour end time add ml potassium iodid solut ml water flask titrat standard volumetr sodium thiosulf solut yellow colour due iodin disappear add drop starch solut continu titrat blue colour disappear vigor shake note potentiometr determin end point permiss number determin carri determin test sampl express result iodin express numer exact concentr mole litr standard volumetr sodium thiosulf solut numer volum millilitr standard volumetr sodium thiosulf solut blank test numer volum millilitr standard volumetr sodium thiosulf solut determin numer mass gram test portion result arithmet determin provid requir repeat satisfi