council direct eec octob control potato ring rot council european commun regard treati establish european econom commun articl thereof regard propos commiss regard opinion european parliament regard opinion econom social committe potato product occupi import place commun agricultur potato yield constantli threaten harm organ protect potato cultiv harm organ product capac maintain agricultur product increas protect measur prevent introduct harm organ territori member state limit effect organ control simultan method commun prevent spread harm organ potato clavibact michiganensi smith davi al ssp sepedonicu spieckermann kotthoff davi al pathogen agent potato ring rot diseas diseas occur part commun limit sourc infect exist consider risk potato cultiv commun effect measur locat diseas determin distribut prevent occur spread found prevent spread control aim erad order ensur measur commun member state addit addit stricter measur provid hindranc movement potato commun laid council direct eec decemb protect measur introduct member state organ harm plant plant product measur notifi member state commiss council direct eec june control potato ring rot laid minimum measur member state potato ring rot signific develop understand potato ring rot diseas detect potato ring rot pathogen applic commun plant health regim commun area intern frontier call examin revis provis direct eec result examin provis direct eec found insuffici specif measur situat direct eec repeal measur adopt measur account diseas remain latend unobserv grow crop store tuber effect prevent product seed potato free infect systemat offici survei locat spread pathogen grow crop import factor pathogen exist winter sown volunt potato plant major sourc infect carri season pathogen spread contamin potato contact infect potato contact plant harvest handl equip transport storag contain contamin organ previou contact infect potato contamin object remain infecti time contamin spread pathogen reduc prevent disinfect object contamin seed potato pose major risk spread pathogen determin detail gener measur stricter addit measur member state prevent introduct pathogen territori desir member state cooper close commiss stand committe plant health hereinaft refer committe adopt direct articl direct concern measur member state clavibact michiganensi smith davi al ssp sepedonicu spieckermann kotthoff davi al potato ring rot hereinaft refer organ order locat determin distribut prevent occurr spread found prevent spread control aim erad articl member state conduct systemat offici survei organ tuber plant potato solanum tuberosum origin territori confirm absenc organ survei case tuber sampl seed potato prefer lot store subject offici offici supervis laboratori test method set annex detect diagnosi organ addit offici offici supervis visual inspect cut tuber sampl case plant survei carri method sampl subject offici offici supervis test number origin stratif time collect sampl decid respons offici bodi mean direct eec base sound scientif statist principl biologi organ take account potato product system member state concern detail thereof submit annual member state commiss view ensur compar level assur member state confirm absenc organ result offici survei provid paragraph notifi year member state commiss detail notif confidenti submit committe accord procedur laid articl direct eec provis adopt accord procedur laid articl direct eec detail survei provid paragraph carri accord sound scientif statist principl detail notif provid paragraph provis adopt accord procedur laid articl direct eec method survei test provid subparagraph paragraph articl member state ensur suspect occurr confirm presenc organ potato plant tuber harvest store market tuber territori report respons offici bodi articl case suspect occurr respons offici bodi member state case report ensur complet offici offici supervis laboratori test method set annex accord condit point annex ii order confirm refut suspect occurr case requir laid point annex ii appli pend confirm refut suspect occurr paragraph case suspect occurr suspect diagnost visual symptom diseas ii posit immunofluoresc test annex posit test identifi respons offici bodi member state prohibit movement lot consign sampl control provid establish identifi risk organ spread step trace origin suspect occurr introduc addit precautionari measur base level estim risk order prevent spread organ measur includ offici control movement tuber plant premis suspect occurr provis adopt accord procedur laid articl direct eec measur refer paragraph provis adopt accord procedur laid articl direct eec test provid paragraph ii articl offici offici supervis laboratori test method set annex confirm presenc organ sampl tuber plant part plant respons offici bodi member state regard sound scientif principl biologi organ product market process system member state design contamin tuber plant consign lot machineri vehicl vessel store unit thereof object includ packag materi sampl place product field tuber plant harvest determin take account provis point annex iii extent probabl contamin pre post harvest contact product link design contamin demarc zone basi design contamin determin extent probabl contamin spread organ take account provis point annex iii member state immedi notifi member state commiss accord provis point annex iii contamin design paragraph detail zone demarc paragraph detail notif confidenti submit committe accord procedur laid articl direct eec result notif paragraph element mention member state detail notif design contamin determin extent probabl contamin demarc zone accord paragraph articl member state prescrib tuber plant design contamin articl test accord articl carri potato stock clonal relat involv contamin test carri tuber plant need determin probabl primari sourc infect extent probabl contamin prefer order degre risk result test design contamin determin extent probabl contamin demarc zone conduct articl articl member state prescrib tuber plant design contamin articl plant control respons offici bodi destroi dispos subject offici supervis measur accord provis point annex iv provid establish identifi risk organ spread member state prescrib tuber plant determin contamin articl plant prejudic outcom test refer articl clonal relat stock control respons offici bodi put dispos point annex iv establish identifi risk organ spread member state prescrib machineri vehicl vessel store unit thereof object includ packag materi design contamin articl determin contamin articl destroi cleans disinfect method point annex iv disinfect object longer consid contamin prejudic measur implement paragraph member state prescrib zone demarc articl seri measur point annex iv implement articl member state prescrib seed potato meet requir direct eec deriv direct line materi obtain offici approv programm found free organ offici offici supervis test method set annex aforesaid test carri case contamin affect seed potato product plant initi clonal select case plant initi clonal select repres sampl basic seed potato earlier propag provis adopt accord procedur laid articl direct eec detail rule applic indent subparagraph paragraph articl rule repres sampl provid indent subparagraph paragraph articl articl member state ban hold handl organ articl prejudic provis direct eec member state author derog measur refer articl direct experiment scientif purpos work variet select provid derog prejudic control organ creat risk spread organ articl member state adopt addit stricter measur requir combat organ prevent spread complianc provison direct eec addit measur mention subparagraph includ prescript seed potato plant offici certifi offici inspect meet requir plant health standard appli case farmer author hold seed potato obtain harvest case produc seed potato plant detail measur notifi member state commiss articl amend annex direct made light develop scientif technic knowledg adopt accord procedur laid articl direct eec articl novemb member state adopt publish provis compli direct immedi inform commiss thereof member state adopt provis refer direct accompani refer time offici public procedur make refer adopt member state member state appli provis novemb member state immedi commun commiss provis nation law adopt field cover direct commiss inform member state thereof articl direct eec repeal effect novemb articl direct address member state luxembourg octob council presid clae oj oj oj oj direct amend commiss direct eec oj oj annex method detect diagnosi ring rot bacterium clavibact michiganensi smith davi al ssp sepedonicu spieckermann kotthof davi al batch potato tuber remov heel end core wash tuber run tap water remov epidermi heel end tuber regularli disinfect scalpel potato peeler disinfect achiev dip peeler ethanol flame carefulli remov conic tissu core heel end knife potato peeler excess vascular tissu minimum remov heel end process hour paragraph conserv longer week visual examin ring rot symptom remov heel end cut tuber transvers observ presenc ring rot symptom squeez tuber express macer tissu vascular tissu earliest symptom slight glassi transluc tissu soften round vascular system heel end vascular ring heel end slightli darker colour normal readili identifi symptom vascular ring yellowish color tuber gentli squeez pillar chees materi emerg vessel exud million bacteria brown vascular tissu develop stage symptom restrict part ring necessarili close heel end gradual extend ring infect progress destruct vascular tissu occur outer cortex separ cortex advanc stage infect crack surfac tuber reddish brown margin secondari fungal bacteri invas mask symptom difficult imposs distinguish advanc ring rot symptom tuber rot prepar sampl gram stain immunofluoresc stain eggplant test homogen heel end complet macer achiev diluent toxic corynebacterium sepedonicum phosphat buffer salin pb ph temperatur addit toxic defloccul advis toxic antifoam agent need appendic excess macer avoid extract bacteria homogen method centrifug minut centrifug supernat minut decant discard supernat macer stand minut tissu debri settl decant supernat disturb sediment filter supernat filter paper whatman held sinter glass filter mm water vacuum pump collect filtrat centrifug tube wash filter steril pb maximum filtrat volum ml centrifug filtrat minut suspend pellet steril phosphat buffer ph appendix give total volum approxim ml divid equal part retain part refer purpos freez lyophil divid part halv half test gram stain eggplant test imper posit sepedonicum control sampl treat separ avoid contamin appli slide eggplant test gram stain prepar gram stain pellet dilut cut tuber show glassi rot suspect symptom sampl edg diseas tissu prepar gram stain sepedonicum cultur natur infect tissu determin sampl typic gram posit coryneform cell gener sepedonicum cell mm long mm wide stain procedur appendix prepar natur infect recent isol cultur show predomin coccoid rod slightli smaller cell oder agar cultur cultur media sepedonicum cell pleomorph coryneform rod give variabl gram reaction cell singl pair characterist elbow typic bend divis occasion irregular group refer palisad chines letter scheme test antiserum strain sepedonicum atcc ncppb ncppb titr includ pb control test slide determin fluorescein isothiocyan anti rabbit immunoglobulin conjug fitc combin specif bacteri cell corynebacterium sepedonicum atcc ncppb ncppb homolog antigen control separ slide natur infect tissu maintain lyophil freez similar control slide figur procedur prepar serial ten fold dilut final pellet distil water figur pipett measur standard volum suffici cover window approxim ml pellet dilut sepedonicum suspens approxim cell ml window multispot slide shown figur cover window sepedonicum antiserum recommend dilut pb ph appendix shown figur pb fitc control work dilut antiserum approxim half titr antiserum dilut includ separ slide prepar dilut incub humid chamber ambient temperatur minut rins carefulli pb ph wash minut pb ph carefulli remov excess moistur cover window fitc conjug dilut determin titr incub dark humid chamber ambient temperatur minut rins wash appli approxim ml phosphat buffer glycerin ph similar mountant ph window cover coverglass appendix examin microscop fit epifluoresc light sourc filter suitabl work fitc magnif suitabl scan replic window diamet angl window perimet observ fluoresc cell posit control determin titr observ fluoresc cell fitc pb control window absent proce test window determin minimum microscop field number morpholog typic fluoresc cell field calcul number ml undilut pellet appendix problem inher immunofluoresc test background popul fluoresc cell atyp morpholog cross react saprophyt bacteria size morpholog similar clavibact michiganensi ssp sepedonicu occur potato pellet fluoresc cell typic size morpholog possibl cross reaction sampl posit immunofloresc test retest antiserum technic limit detect method situat cell ml undilut pellet sampl count typic cell detect limit neg ssp sepedonicu commit eggplant test neg immunofluoresc test identifi sampl morpholog typic fluoresc cell found sampl consid contamin clavibact michiganensi ssp sepedonicu eggplant test requir posit immunofluoresc test identifi sampl morpholog typic fluoresc cell found sampl posit immunofluoresc test identifi antisera consid potenti contamin clavibact michiganensi ssp sepedonicu eggplant test requir sampl consid potenti contamin eggplant test cultur detail appendix distribut pellet eggplant leaf stage appendix method slit inocul support pot horizont block expand polystyren piec cm deep cm wide cm long remov surfac figur adequ cm pot strip steril aluminium foil stem block sampl test plant held place rubber band block scalpel make longitudin slightli diagon cut cm long approxim quarter stem diamet deep cotyledon leaf hold slit open scalpel blade point paint inoculum eyelin fine artist brush charg pellet distribut remaind pellet eggplant seal cut steril vaselin ml syring barrel slit inocul ii hold plant finger pipett drop approxim ml suspend pellet stem cotyledon leaf steril scalpel make diagon angl approxim slit cm long approxim stem thick deep start cut pellet drop seal cut steril vaselin syring barrel syring inocul water eggplant dai prior inocul reduc turgor pressur inocul eggplant stem cotyledon syring fit hypoderm needl distribut pellet eggplant inocul plant sepedonicum cultur natur infect tuber tissu inocul method inocul plant steril pb inocul method incub plant condit appendix dai examin regularli symptom dai count number plant show symptom sepedonicum leaf wilt eggplant commenc margin intervein flaccid wilt tissu initi dark green mottl turn paler necrot intervein wilt greasi water soak appear necrot tissu bright yellow margin plant necessarili kill longer period symptom develop greater chanc surviv plant outgrow infect suscept young eggplant sensit low popul sepedonicum older plant necess plant leaf stage wilt induc popul bacteria fungi present tuber tissu pellet includ erwinia carotovora subsp carotovora carotovora subsp atroseptica phoma exigua var foveata larg popul saprophyt bacteria wilt distinguish caus sepedonicum leav plant wilt rapidli prepar gram stain batch eggplant show symptom section wilt leaf tissu stem tissu plant isol suitabl nutrient media surfac disinfect eggplant leav stem wipe ethanol circumst grow condit optim sepedonicum exist latent infect eggplant incub dai infect possibl result stunt lack vigour inocul plant test consid posit consid test essenti compar growth rate eggplant test plant steril pb inocul control monitor environment condit glasshous recommend test excis stem inocul site remov leav macer stem pb ph half pellet perform gram stain test half perform eggplant test gram stain test posit sepedonicum cultur steril pb control symptom observ subsequ test sampl consid neg isol sepedonicum diagnosi confirm sepedonicum isol identifi sepedonicum fastidi organ isol symptomat tissu outgrown rapidli grow saprophyt bacteria isol directli tuber tissu pellet recommend eggplant provid excel select enrich medium growth sepedonicum provid excel confirmatori host test isol made symptomat potato tuber eggplant macer eggplant stem carri streak suspens media formula appendix nutrient dextros agar subcultur yeast pepton glucos agar nutrient yeast dextros agar yeast extract miner salt agar incub dai sepedonicum slow grow produc pin point cream dome coloni dai streak establish puriti growth rate improv subcultur typic coloni creami white ivori round smooth rais convex dome mucoid fluidal entir edg mm diamet identif gram posit coryneform bacteria coloni charact similar sepedonicum isol healthi diseas potato eggplant context sepedonicum identifi test test eggplant test nutrit physiolog test appendix oxid ferment test oxidas test growth ureas product aesculin hydrolysi starch hydrolysi toler sodium chlorid solut indol test catalas test product citrat util gelatin hydrolysi acid glycerol lactos rhamnos salicin gram stain test includ sepedonicum control nutrit physiolog test made inocula nutrient agar subcultur morpholog comparison made nutrient dextros agar cultur test cell popul adjust cell ml titr similar sepedonicum cultur eggplant test cell popul adjust cell ml eggplant test made plant test organ sepedonicum cultur steril water control pure cultur typic wilt obtain dai plant show sympton time incub total dai temperatur conduc eggplant growth exceed appendix dai symptom present cultur confirm pathogen form corynebacterium sepedonicum test sepedonicum inert weakli oxid oxidas catalas nitrat reduct ureas activ product indol product citrat util starch hydrolysi weak growth growth nacl gelatin hydrolysi aesculin hydrolysi acid glycerol lactos weak rhamnos salicin appendix formul macer fluid recommend lelliott sellar silicon antifoam ms compound hopkin amp william cat chadwel heath essex england ml lubrol flake ici tetra sodium pyrophosph phosphat buffer salin ph appendix litr appendix buffer phosphat buffer salin ph buffer tuber tissu macer na hpo kh po nacl distil water litr phosphat buffer salin ph buffer dilut antisera wash slide na hpo nah po nacl distil water litr phosphat buffer glycerin ph buffer mountant enhanc fluoresc test na hpo nah po glycerol ml distil water ml appendix gram stain procedur hucker modif doetsch crystal violet solut dissolv crystal violet ml ethanol dissolv ammonium oxal ml distil water mix solut lugol iodin iodin potassium iodid distil water ml grind solid pestl mortar add water stir dissolv close contain safranin counterstain solut stock solut safranin ethanol ml mix store dilut obtain work solut stain procedur prepar smear air dry heat fix flood slide crystal violet solut minut wash briefli tap water flood lugol iodin minut wash tap water blot dry decolour ethanol ad dropwis colour remov immers gentl agit second wash tap water blot dry flood safranin solut second wash tap water blot dry gram posit bacteria stain violet blue gram neg bacteria stain pink red appendix determin popul posit cell surfac area window multispot slide diamet window surfac area object field diamet field calcul direct measur formula field coeffici depend ocular type vari tube coeffici magnif object pfrom ppcount number typic fluoresc cell field calcul number typic fluoresc cell window calcul number typic fluoresc cell ml pellet volum pellet window pellet dilut factor appendix eggplant cultur sow seed eggplant solanum melongena cv black beauti pasteur seed compost transplant seedl fulli expand cotyledon dai pasteur pot compost eggplant leaf stage leav fulli uncurl eggplant grown glasshous environment condit dai length hour natur dai length greater temperatur dai night nb sepedonicum grow temperatur gt night temperatur fall chromophor damag silveri necrosi occur root damag caus sciarid larva overcom applic insecticid eggplant cv black beauti obtain ab hammenhoeg froe hammenhoeg sweden hurst seed avenu road witham essex cm dx england asgro italia sp corso lodi milan kuepper mitteldeutsch samen gmbh hessenr eschweg appendix media growth isol sepedonicum nutrient agar na difco bacto nutrient agar distil water manufactur rate steril autoclav minut nutrient dextros agar nda difco bacto nutrient agar glucos monohydr steril autoclav minut yeast pepton glucos agar ypga difco bacto yeast extract difco bacto pepton glucos monohydr difco bacto purifi agar distil water litr steril litr volum medium autoclav minut yeast extract miner salt medium ygm difco bacto yeast extract glucos monohydr hpo kh po mgso mnso nacl feso difco bacto purifi agar distil water litr steril litr volum medium autoclav minut appendix nutrit physiolog test identif sepedonicum media incub examin dai growth occur incub dai oxid ferment test hugh amp leifson test basal medium kcl mgso nh po difco bacto pepton difco bacto purifi agar glucos monohydr bromothymol blue distil water litr mix adjust ph koh dispens pyrex cultur tube mm mm ml capac ml ml volum steril autoclav minut stab inocul ml ml tube cultur asept add ml steril liquid paraffin ml tube incub tabl oj kovac kovac oxidas reagent aqueou solut tetramethyl paraphenylenediamin dihydrochlorid bdh distil water reagent freshli made ml volum store brown glass bottl week place drop reagent filter paper clean petri dish immedi rub test cultur nutrient agar platinum loop posit reaction develop purpl color second cultur time second weakli posit nb essenti platinum loop na cultur trace iron high sugar content growth medium give fals posit result acid product lactos rhamnos salicin glycerol prepar hugh amp leifson medium glucos distribut ml volum tube steril autoclav minut molten base asept add ml filter steril aqueou solut glycerol lactos rhamnos salicin mix carefulli posit reaction colour chang blue green yellow product acid catalas test place drop hydrogen peroxid volum clean slide emulsifi loop cultur platinum loop posit reaction product oxygen bubbl drop presenc catalas nitrat reductas activ denitrif bradburi cultur medium kno nitrit free difco bacto yeast extract hpo distil water litr dispens ml volum ml bottl steril autoclav minut reagent acet acid litr reagent naphthylamin acet acid litr inocul nitrat medium duplic test dai ad drop lugol iodin ml reagent ml reagent medium turn reddish add approxim mg zinc powder observ colour reaction posit reaction colour reaction stage stage reduct nitrat colourless red reduct nitrat nitrit nitrat reductas red reduct nitrat nitrit denitrif nitrat nitrit reductas colourless colourless ureas product lelliott basal medium oxoid urea agar base cm distil water ml steril autoclav minut cool molten base asept add ml filter steril aqueou urea solut oxoid sr mix distribut ml volum steril tube mm set slope good butt posit reaction yellow orang medium develop cherri red magenta pink color ureas activ occur util citrat christensen skerman citrat agar base merck distil water litr mix dissolv heat dispens ml volum urea medium steril autoclav minut set slope posit reaction citrat util chang colour medium orang red hydrogen sulphid product ramamurthi medium difco bacto trypton hpo nacl distil water litr dissolv dispens ml volum mm tube steril autoclav minut inocul asept suspend lead acet paper merck lip tube hold place cap incub dai posit reaction product trypton develop black brown color test paper indol product ramamurthi medium test remov lead acet paper add ml diethyl ether shake gentli layer separ minut add ml kovac reagent merck carefulli insid slope tube posit reaction presenc indol develop red colour yellow layer ether aqueou fraction growth ar ramamurthi medium difco bacto nutrient broth distil water litr mix dissolv distribut ml volum tube steril autoclav minut inocul incub posit reaction growth growth sodium chlorid ramamurthi medium difco bacto nutrient broth nacl distil water litr mix dissolv distribut ml volum tube steril autoclav minut posit reaction growth gelatin hydrolysi lelliott bill amp hayward medium difco bacto gelatin distil water litr mix dissolv heat distribut ml volum tube steril autoclav minut posit reaction liquefact gelat held minut starch hydrolysi medium difco bacto nutrient agar molten litr difco bacto solubl starch mix steril autoclav minut pour plate spot inocul plate good growth occur dai remov part growth flood lugol iodin posit reaction starch hydrolysi clear zone bacteri growth remaind medium stain purpl aesculin hydrolas activ sneath amp collin medium difco bacto pepton aesculin ferric citrat merck sodium citrat distil water litr mix dissolv distribut ml volum tube steril autoclav minut medium clear bluish fluoresc posit reaction aesculin hydrolysi develop brown colour disappear fluoresc check ultraviolet lamp refer bradburi isol preliminari studi bacteria plant rev pl path dinesen extract diagnosi corynebacterium sepedonicum diseas potato tuber eppo bull doetsch determin method light microscopi manual method gener bacteriolog american societi microbiolog washington hugh leifson taxonom signific ferment versu oxid metabol carbohydr gram neg bacteria bact jans van vaerenbergh interpret ec method detect latent ring rot infect corynebacterium sepedonicum potato eppo bull pp kovac identif pseudomona pyocyanea oxidas reaction natur lond lelliott plant pathogen coryneform bacteria appl bact lelliott bill hayward determin scheme fluoresc plant pathogen pseudomonad appl bact lelliott sellar detect latent ring rot corynebacterium sepedonicum spiek kotth skapt burkh potato stock eppo bull ramamurthi compar studi gram posit phytopathogen bacteria relationship corynebacteria mem cornel agric exp sta pp skerman guid identif genera bacteria ed william wilkin compani baltimor sneath collin studi test reproduct laboratori report pseudomona work parti antoni van leeuwenhoek altern method extract dinesen evid jans van vaerenberg freez reduc viabil corynebacterium sepedonicum suspens pellet glycerol overcom problem annex ii suspect occurr posit immunofluoresc test identifi method set annex confirm refut complet method await retent conserv tuber plant sampl remain extract addit prepar immunofluoresc slide complet method case posit confirm organ retent conserv materi paragraph sampl infect eggplant materi inocul tuber plant extract isol cultur organ month notif procedur articl annex iii element consid determin extent probabl contamin articl includ tuber plant grown place product design contamin articl place product premis product link tuber plant design contamin articl includ share product equip facil directli common contractor tuber plant produc place product refer previou indent present place product period tuber plant design contamin articl present premis place product refer indent central store handl potato place product machineri vehicl vessel store unit thereof object includ packag materi contact tuber plant design contamin articl previou month tuber plant store contact structur object list previou indent prior cleans disinfect structur object includ result test articl tuber plant clonal origin tuber plant design contamin articl investig contamin probabl element consid determin spread articl includ proxim place product grow potato host plant common seed potato stock detail notif refer subparagraph articl includ potato consign lot design contamin certif prescrib articl direct eec passport registr number varieti seed potato stock case descript element design contamin zone demarc avail extract prepar immunofluoresc slide infect eggplant materi isol cultur organ test posit confirm organ made annex iv offici supervis measur refer articl dispos tuber plant design contamin articl industri process direct deliveri process plant wast dispos facil establish identifi risk organ spread system disinfect storag area depart vehicl measur provid establish identifi risk organ spread measur notifi commiss member state dispos tuber plant determin contamin articl refer articl control respons offici bodi member state ware potato intend consumpt pack readi direct deliveri repack intend direct deliveri ware potato intend industri process intend direct deliveri process plant wast dispos disinfect facil dispos provid establish identifi risk organ spread method cleans disinfect object refer articl establish identifi risk organ spread emploi supervis respons offici bodi member state seri measur implement member state demarc zone establish articl refer articl includ place product design contamin articl field design contamin articl grow year year design contamin measur elimin volunt potato plant natur found host plant organ potato tuber plant true seed natur found host plant organ crop identifi risk organ surviv spread plant field found free volunt potato plant consecut grow year potato crop season period preced indent offici certifi seed potato plant ware product offici survei detail articl conduct potato crop season succeed refer previou indent rotat cycl offici certifi seed potato plant seed ware product offici survei detail articl conduct ii grow year design contamin measur elimin volunt potato plant natur found host plant organ field laid maintain bare fallow perman pastur frequent close cut intens graze potato crop season period preced indent offici certifi seed potato plant seed ware product offici survei detail articl conduct field grow year design contamin potato tuber plant true seed natur found host plant organ plant measur elimin volunt potato plant offici certifi seed potato plant ware product condit respons offici bodi satisfi risk volunt potato plant natur found host plant organ elimin grow year preced indent offici certifi seed potato plant seed ware product grow year refer previou indent measur elimin volunt potato plant natur found host plant organ offici survei detail articl conduct offici certifi seed potato plant ware product grow year design contamin grow crop inspect time volunt potato plant test organ immedi design contamin articl subsequ grow year includ permiss potato crop season field design contamin detail paragraph machineri storag facil place product involv potato product cleans disinfect method product system complet replac grow medium tuber plant true seed plant product unit subject offici supervis measur elimin organ remov potato solanac materi includ complet chang grow medium cleans disinfect product unit equip subsequ grant approv potato product respons offici bodi potato product offici certifi seed potato mini tuber micro plant deriv test sourc demarc zone prejudic measur detail member state immedi grow season design contamin ensur supervis respons offici bodi premis grow store handl potato tuber premis oper potato machineri contract requir cleans disinfect machineri store premis method requir plant certifi seed potato crop zone requir separ handl harvest seed stock ware premis zone conduct offici survei detail articl establish programm replac seed potato stock period time measur implement registr number produc collect warehous dispatch centr demarc zone notifi annual member state commiss