commiss direct septemb approxim law member state relat method analysi check composit cosmet product eec commiss european commun regard treati establish european econom commun regard council direct eec juli approxim law member state relat cosmet product amend direct eec articl thereof direct eec offici test cosmet product aim ensur condit laid commun provis composit cosmet product satisfi method analysi laid quickli step attain object definit method commiss direct eec eec step consist definit method determin dichloromethan trichloroethan identif determin quinolin ol bi hydroxyquinolinium sulphat determin ammonia identif determin nitromethan identif determin mercaptoacet acid hair wave hair straighten depilatori product identif determin hexachlorophen inn tion tosylchloramid sodium inn determin total fluorin dental cream identif determin organomercuri compound determin alkali alkalin earth sulphid measur provid direct accord opinion committe adapt direct eec technic progress adopt direct articl member state step ensur offici test cosmet product determin dichloromethan trichloroethan identif determin quinolin ol bi hydroxyquinolinium sulphat determin ammonia identif determin nitromethan identif determin mercaptoacet acid hair wave hair straighten depilatori product identif determin hexachlorophen inn determin tosylchloramid sodium inn determin total fluorin dental cream identif determin organomercuri compound oj oj oj oj determin alkali alkalin earth sulphid perform accord method annex hereto articl member state bring forc law regul administr provis compli direct decemb forthwith inform commiss thereof articl direct address member state brussel septemb commiss fran andriessen member commiss annex determin dichloromethan trichloroethan scope field applic method describ determin dichloromethan methylen chlorid trichloroethan methyl chloroform cosmet product solvent definit dichloromethan trichloroethan content sampl determin method express percentag mass principl method ga chromatographi chloroform intern standard reagent reagent analyt qualiti chloroform chcl carbon tetrachlorid ccl dichloromethan ch cl trichloroethan ch ccl aceton nitrogen apparatu usual laboratori apparatu ga chromatograph fit thermal conduct detector transfer bottl ml sampl method pressur ga syring sampl method procedur pressur sampl weigh sampl accur stopper conic flask introduc accur weigh quantiti chloroform intern standard equival presum quantiti dichloromethan trichloroethan contain sampl mix throroughli oj pressur sampl sampl method sampl chapter refin transfer sampl transfer bottl introduc transfer bottl volum chloroform intern standard equival presum quantiti dichloromethan trichloroethan contain sampl mix rins dead volum valv ml carbon tetrachlorid dry determin accur ad mass intern standard differ fill syring sampl nozzl syring purg nitrogen residu remain inject chromatograph sampl surfac valv transfer piec rins time aceton requir hypoderm syring dri nitrogen analysi measur transfer bottl measur bottl chromatograph condit precolumn tube stainless steel length mm diamet mm pack materi analyt column pack column stationari phase made hallcomid chromosorb column yield resolut quot quot equal gt pic file quot quot gt retent time minut peak width half height millimetr chart speed millimetr minut exampl column yield result sought gt pic file quot quot gt temperatur condit vari function apparatu exampl set gt pic file quot quot gt mixtur establish respons factor make accur weigh mixtur stopper conic flask dichloromethan trichloroethan chloroform calcul calcul respons factor substanc quot quot rel substanc quot quot select intern standard substanc quot quot kp respons factor mp mass mixtur ap peak area substanc quot quot ka respons factor made equal uniti ma mass mixtur aa peak area gt pic file quot quot gt exampl respons factor obtain chloroform gt pic file quot quot gt calcul dichloromethan trichloroethan present sampl analyz ma mass gram chloroform introduc ms mass gram sampl analyz aa area chloroform peak area dichloromethan peak area trichloroethan peak gt pic file quot quot gt repeat dichloromethan trichloroethan content differ result determin carri parallel sampl exce absolut norm iso identif determin quinolin ol bi hydroxyquinolinium sulphat scope field applic method describ identif quantit determin quinolin ol sulphat definit quinolin ol bi hydroxyquinolinium sulphat content sampl determin method express percentag mass quinolin ol principl identif identif thin layer chromatographi determin determin carri spectrophotometri nm complex obtain reaction fehl solut reagent reagent analyt puriti quinolin ol benzen view toxic great care work benzen chloroform aqueou sodium hydroxid solut copper sulphat pentahydr potassium sodium tartrat hydrochlor acid sulphur acid sodium hydroxid solut ethanol butan ol glacial acet acid hydrochlor acid quot celit quot equival standard solut weigh mg quinolin ol ml standard flask dissolv sulphur acid make mark sulphur acid weigh mg quinolin ol ml standard flask dissolv ethanol make mark ethanol mix fehl solut solut weigh copper sulphat pentahydr ml standard flask dissolv water make mark water mix solut weigh potassium sodium tartrat ml standard flask dissolv ml water add ml sodium hydroxid make mark water mix immedi pipett ml solut ml solut ml standard flask make mark mix elut solvent thin layer chromatographi butan ol acet acid water ii chloroform acet acid dichloro chloroin ino cyclohexa dienon solut ethanol sodium carbon solut water ethanol solut water disodium dihydrogen ethylenediaminetetraacet solut water buffer solut ph weigh potassium dihydrogenorthophosph anhydr dipotassium hydrogenorthophosph trihydr litr standard flask make mark water prepar thin layer plate readi made thin layer plate thick mm merck kieselgel equival sprai ml reagent dry apparatu ml round bottom flask ground glass neck standard flask graduat pipett ml bulb pipett ml separ funnel ml pleat filter paper diamet mm rotari evapor reflux condens ground glass neck spectrophotomet optic cell mm path length stirrer hotplat glass chromatographi column dimens mm long diamet mm constrict lower end glass wool plug adaptor upper end applic pressur procedur identif liquid sampl ph part test sampl adjust spot start line pretreat silica gel thin layer plate standard solut spot point start line plate develop eluent solvent front advanc mm plate dri minut uv lamp nm quinolin ol spot fluoresc yellow sprai plate sodium carbon solut dry sprai dichloro caloroimino cyclohexa dienon solut quinolin ol visibl blue spot solid sampl cream dispers sampl ml buffer solut transfer ml chloroform separ funnel shake separ chloroform layer aqueou layer extract ml chloroform evapor combin filter chloroform extract dryness ml round bottom flask rotari evapor dissolv residu ml chloroform spot solut obtain silica gel thin layer plate accord method onward appli standard solut plate continu determin liquid sampl weigh sampl ml round bottom flask add ml sulphur acid solut evapor mixtur dryness reduc pressur dissolv residu ml warm water transfer ml standard flask rins time ml water make ml water mix pipett ml solut ml separ funnel add ml fehl solut extract quinolin ol copper complex oxin copper iso obtain time ml chloroform filter collect chloroform layer ml standard flask make mark chloroform shake measur optic densiti yellow solut chloroform nm solid sampl cream weigh sampl ml round bottom flask add ml benzen ml hydrochlor acid boil content flask reflux stir minut transfer content flask ml separ funnel rins ml hcl transfer aqueou phase round bottom flask wash benzen phase ml hydrochlor acid case emuls imped treatment mix sampl celit form freeli flow powder transfer mixtur small portion glass chromatographi column addit tamp column pack mixtur transfer column elut hydrochlor acid ml eluat obtain approxim minut elut perform slight nitrogen pressur elut ensur hydrochlor acid column pack ml eluat treat evapor collect aqueou phase eluat dryness rotari evapor reduc pressur dissolv residu ml sodium hydroxid solut add ml fehl solut transfer content flask ml separ funnel rins flask ml chloroform shake filter chloroform phase ml standard flask repeat extract time ml chloroform filter chloroform phase collect ml flask make mark chloroform shake measur optic densiti yellow solut chloroform nm standard curv ml round bottom flask ml aqueou ethanol pipett ml portion standard solut mg quinolin ol proce calcul liquid sampl gt pic file quot quot gt milligram quinolin ol standard curv mass milligram test portion solid sampl cream gt pic file quot quot gt milligram quinolin ol standard curv mass milligram test portion repeat content quinolin ol differ result determin carri parallel sampl exce absolut determin ammonia scope field applic method describ determin free ammonia cosmet product definit ammonia content sampl determin accord method express percentag mass ammonia principl barium chlorid solut ad test portion cosmet product dilut aqueou methanol medium precipit form filter centrifug procedur avoid loss ammonia steam distil ammonium salt carbon hydrogencarbon fatti acid except ammonium acet ammonia steam distil filtrat supernat determin potentiometr titrat reagent reagent analyt puriti methanol barium chlorid dihydr solut orthobor acid solut sulphur acid standard solut anti foam liquid sodium hydroxid standard solut indic requir mix ml methyl red solut ethanol ml methylen blue solut water norm iso apparatu usual laboratori apparatu centrifug stopper ml bottl steam distil apparatu potentiomet indic glass electrod dimercuri dichlorid calomel refer electrod procedur weigh ml standard flask mass sampl mg maximum ammonia add ml water ml methanol ml barium chlorid solut make ml methanol mix leav overnight refriger filter centrifug cold solut close tube minut obtain clear filtrat supernat layer pipett ml clear solut steam distil apparatu ml antifoam liquid distil collect ml distil ml beaker ml standard sulphur acid ml indic back titrat excess acid standard sodium hydroxid solut nb potentiometr determin collect ml distil ml beaker ml orthobor acid solut titrat standard sulphur acid record neutral curv calcul calcul case back titrat volum millilitr sodium hydroxid solut actual molar actual molar factor sulphur acid solut mass milligram test portion gt pic file quot quot gt calcul case direct potentiometr titrat volum millilitr sulphur acid solut actual molar mass milligram test portion gt pic file quot quot gt repeat content ammonia differ result determin carri parallel sampl exce absolut identif determin nitromethan scope field applic method suitabl identif determin nitromethan cosmet product pack aerosol dispens definit nitromethan content sampl determin method express percentag mass nitromethan total aerosol dispens content principl nitromethan identifi colour reaction nitromethan determin ga chromatograph addit intern standard identif reagent reagent analyt puriti sodium hydroxid solut folin reagent dissolv sodium dihydro dioxonaphthalen sulphon water dilut ml procedur ml sampl add ml ml violet color presenc nitromethan determin reagent reagent analyt qualiti norm iso chloroform intern standard dimethylheptan intern standard ethanol nitromethan chloroform refer solut tare ml volumetr flask introduc mg chloroform accur reweigh flask content make ml ethanol weigh calcul percentag mass chloroform solut dimethylheptan refer solut make similar manner chloroform refer solut weigh mg dimethylheptan ml volumetr flask apparatu ga chromatograph flame ioniz detector apparatu sampl aerosol transfer bottl microsyring connector chapter ii annex commiss direct eec decemb usual laboratori apparatu procedur prepar sampl ml tare transfer bottl purg evacu procedur chapter ii abovement direct introduc ml intern standard solut ml glass syring needl adapt transfer piec techniqu paragraph chapter ii abovement commiss direct reweigh determin quantiti introduc techniqu transfer bottl content aerosol dispens sampl reweigh determin quantiti sampl transfer mix inject microsyring make inject prepar standard ml volumetr flask accur weigh mg nitromethan mg chloroform mg dimethylheptan make volum ethanol mix place ml solut ml volumetr flask make volum ethanol inject microsyring make inject ga chromatograph condit column part didecyl phthalat ga chrom pack ucon hb ga chrom pack oj prepar combin column yield resolut quot quot equal gt pic file quot quot gt retent time minut peak width half height millimetr chart speed millimetr minut exampl part yield requir resolut column materi stainless steel length diamet mm pack didecyl phthalat ga chrom mesh column materi stainless steel length diamet mm pack ucon hb ga chrom mesh detector suitabl sensit set electromet flame ioniz detector temperatur condit found suitabl inject port detector column depend individu column apparatu suitabl ga suppli carrier ga nitrogen pressur bar flow ml min detector suppli maker detector calcul respons factor nitromethan calcul refer intern standard quot quot repres nitromethan kn respons factor mass gram mixtur peak area quot quot repres intern standard chloroform dimathylheptan mass gram mixtur peak area gt pic file quot quot gt kn function apparatu concentr nitromethan sampl quot quot repres nitromethan kn respons factor sn peak area quot quot repres intern standard chloroform dimethylheptan mc mass gram mixtur sc peak area mass gram aerosol transfer nitromethan sampl gt pic file quot quot gt repeat nitromethan content differ result determin carri parallel sampl exce absolut identif determin mercaptoacet acid hair wave hair straighten depilatori product scope field applic method describ identif determin mercaptoacet acid hair wave hair straighten depilatori product reduc agent present definit mercaptoacet acid content sampl determin method express percentag mass mercaptoacet acid principl mercaptoacet acid identifi spot test thin layer chromatographi determin iodometri ga chromatographi identif identif spot test reagent reagent analyt puriti lead di acet paper hydrochlor acid solut volum concentr hydrochlor acid volum water procedur identif mercaptoacet acid mean colour reaction lead di acet place drop sampl analyz lead di acet paper intens yellow colour appear mercaptoacet acid present sensit character inorgan sulphid formul hydrogen sulphid acidif introduc test tube milligram sampl studi add ml distil water ml hydrochlor acid hydrogen norm iso sulphid recogniz smell evolv black lead sulphid precipit form lead di acet paper sensit ppm character sulphit format sulphur dioxid acidif proce bring boil sulphur dioxid recogniz smell reduc properti respect permangan ion identif thin layer chromatographi reagent reagent state analyt puriti mercaptoacet acid thioglycol acid minimum puriti assai iodometri dithiodi acet acid minimum puriti assai iodometri mercaptopropion acid thiolact acid minimum puriti assai iodometri mercaptopropion acid minimum puriti assai iodometri mercaptopropan diol thioglycerol minimum puriti assai iodometri thin layer plate silica gel readi prepar mm thick thin layer plate aluminium oxid merck equival gt pic file quot quot gt ethyl acet chloroform diisopropyl ether carbon tetrachlorid acet acid glacial potassium iodid solut water platinum tetrachlorid solut water elut solvent ethyl acet chloroform diisopropyl ether acet acid volum chloroform acet acid volum detect reagent mix immedi equal volum solut solut bromin solut dissolv bromin ml carbon tetrachlorid fluorescein solut dissolv mg fluorescein ml ethanol hexaammonium heptamolybd solut water refer solut mercaptoacet acid solut water dithiodi acet acid solut water mercaptopropion acid solut water mercaptopropion acid solut water mercaptopropan diol solut water apparatu usual apparatu thin layer chromatographi procedur treatment sampl acidifi ph drop hydrochlor acid filter case advis dilut sampl acidifi hydrochlor acid dilut elut place plate sampl solut litr refer solut dry carefulli gentl current nitrogen elut plate solvent dry plate quickli minim oxid thiol detect sprai plate reagent plate sprai reagent treat bromin vapour tank small beaker reagent spot visibl detect sprai reagent satisfactori dry time thin layer exceed minut interpret compar rf valu colour refer solut standard rf valu rough guid compar depend state activ thin layer time chromatograph temperatur chromatographi tank exampl rf valu obtain silica gel layer gt pic file quot quot gt determin nb determin start iodometr procedur iodometri principl determin perform oxid quot sh quot group iodin acid medium equat gt pic file quot quot gt reagent iodin standard solut nb determin mercaptoacet acid carri unus product freshli open contain order prevent oxid apparatu usual laboratori equip procedur accur weigh quantiti sampl ml stopper conic flask ml distil water add ml hydrochlor acid ph solut titrat iodin solut yellow colour appear indic starch solut carbon tetrachlorid desir calcul mercaptoacet acid content calcul formula gt pic file quot quot gt mass gram test portion volum iodin solut remark result calcul mercaptoacet acid author maximum concentr point carri determin result equal permit maximum concentr identif reveal presenc reduc agent carri ga chromatograph determin ga chromatographi principl mercaptoacet acid separ excipi precipit cadmium di acet solut methyl diazomethan prepar situ advanc diethyl ether solut methyl deriv mercaptoacet acid measur ga liquid chromatographi methyl octano intern standard reagent reagent analyt qualiti mercaptoacet acid gt pic file quot quot gt methanol cadmium di acet dihydr solut water methyl octano solut methanol acet buffer solut ph sodium acet trihydr acet acid glacial deminer water give final volum litr hydrochlor acid solut methanol freshli prepar methyl nitro nitrosoguanidin sodium hydroxid solut iodin standard solut diethyl ether diazomethan solut prepar methyl nitrosotoluen sulfonamid fieser reagent organ synthesi wilei solut obtain diazomethan ml diethyl ether diazomethan toxic unstabl ga experi carri power hood ground glass apparatu avoid special kit purpos apparatu usual laboratori equip apparatu prepar diazomethan situ methyl fale jaouni babashak analyt chem apparatu advanc prepar diazomethan fieser prepar sampl weigh accur ml centrifug tube sampl give presum quantiti mg mercaptoacet acid acidifi drop hydrochlor acid obtain ph add ml deminer water ml acet buffer solut check ph paper ph add ml cadmium di acet solut wait minut centrifug minut remov supernat liquid insolubl fat case cream product fat confus thiol collect compact mass bottom tube check precipit occur drop cadmium di acet solut ad supernat earlier identif reveal reduc agent thiol check iodometri thiol present supernat liquid exce initi quantiti introduc ml methanol centrifug tube precipit fine dispers precipit stir rod centrifug minut pour supernat check absenc thiol wash precipit time procedur centrifug tube add ml methyl octaro solut ml hydrochlor acid methanol complet dissolv thiol insolubl matter persist excipi solut quot quot aliquot solut check iodometr thiol content obtain methyl methyl carri situ prepar previous prepar diazomethan solut methyl situ methyl apparatu ml ether introduc solut quot quot methyl method mg methyl nitro nitrosoguanidin minut ether solut yellow excess diazomethan transfer sampl solut ml bottl airtight stopper place refriger overnight methyl sampl simultan methyl previous prepar diazomethan solut introduc ml stopper flask ml diazomethan solut solut quot quot leav refriger overnight prepar standard prepar standard solut mercaptoacet acid strength mg pure mercaptoacet acid ml solut quot quot precipit assai methyl ga chromatograph condit column type stainless steel length diamet mm pack didecyl phthalat chromosorb waw mesh detector flame ioniz suitabl sensit set electromet flame ioniz detector ga suppli carrier ga nitrogen pressur bar flow ml min auxiliari ga hydrogen pressur bar flow ml min detector suppli maker apparatu temperatur condit injector detector column record chart speed mm min quantiti inject carri inject condit chromatographi guid permit achiev resolut quot quot equal gt pic file quot quot gt retent time minut peak width half height millimetr chart speed millimetr minut recommend chromatographi termin regul temperatur rate minut elimin substanc liabl interfer subsequ measur calcul coeffici proportion mercaptoacet acid calcul respect methyl octano basi standard mixtur quot quot repres mercaptoacet acid kt respons factor mass milligram mixtur peak area quot quot repres methyl octano mass milligram mixtur peak area gt pic file quot quot gt coeffici vari apparatu concentr mercaptoacet acid present sampl quot quot repres mercaptoacet acid kt respons factor st peak area quot quot repres methyl octano mc mass milligram mixtur sc peak area mass milligram initi test portion mercaptoacet acid present sampl gt pic file quot quot gt repeat mercaptoacet acid content differ result determin carri parallel sampl exce absolut identif determin hexachlorophen identif scope field applic method suitabl cosmet product principl hexachlorophen sampl extract ethyl acet identifi thin layer chromatographi reagent reagent analyt puriti sulphur acid solut celit aw ethyl acet norm iso elut solvent benzen glacial acet acid visual agent rhodamin solut dissolv mg rhodamin mixtur ml diethyl ether ml absolut ethanol ml water visual agent ii dibromo chloroimino cyclohexa dienon solut dissolv mg dibromo chloroimino cyclohexa dienon ml methanol prepar fresh daili sodium carbon solut dissolv sodium carbon ml deminer water refer solut hexachlorophen solut ethyl acet apparatu kiesel gel tlc plate mm equival usual tlc equip bath thermostat hold chromatographi tank prepar test sampl mix homogen sampl celit aw ml sulphur acid dry hour cool fine powder dri residu extract ml ethyl acet time centrifug extract combin ethyl acet layer evapor dissolv residu ml ethyl acet procedur place test sampl solut refer solut tlc plate satur tank elut solvent place tlc plate tank elut mm remov tlc plate dry ventil oven temperatur visual hexachlorophen spot thin layer plate visual sprai visual agent evenli plate minut examin plate uv light nm sprai dibromo chloroimino cyclohexa dienon solut visual agent ii evenli plate subsequ sprai plate sodium carbon solut examin plate daylight minut dry room temperatur interpret visual agent hexachlorophen reveal bluish spot yellow orang fluoresc background rf approxim visual agent ii hexachlorophen reveal sky blue turquois colour spot white background rf approxim determin scope field applic method appli cosmet product definit hexachlorophen content sampl determin method express percentag mass hexachlorophen principl hexachlorophen determin convers methyl deriv ga chromatograph electron captur detector reagent reagent analyt puriti ethyl acet methyl nitroso toluenesulphonamid diazald diethyl ether methanol ethoxyethoxi ethanol carbitol formic acid potassium hydroxid aqueou solut prepar fresh daili hexan spectroscopi bromochlorophen standard tetrachloro thiodiphenol standard trichloro hydroxi diphenyl ether standard aceton sulphur acid celit aw formic acid ethyl acet solut hexachlorophen apparatu usual laboratori glasswar mini apparatu prepar diazomethan analyt chem ga chromatograph equip ni sourc electron captur detector procedur prepar standard solut standard chosen interfer substanc contain excipi product analyz standard suitabl accur weigh mg standard mg hexachlorophen ml volumetr flask make volum ethyl acet solut dilut ml solut ml ethyl acet solut accur weigh mg standard ml volumetr flask make volum ethyl acet solut prepar sampl accur weigh homogen sampl mix ml sulphur acid ml aceton celit aw air dry mixtur minut steam bath dry half hour ventil oven cool fine powder residu transfer glass column wide rang product type hexachlorophen present import check recoveri hexachlorophen sampl procedur record result recoveri low modif chang solvent benzen ethylacet introduc agreement parti concern elut ethyl acet collect ml add ml intern standard solut solut methyl sampl cool reagent apparatu hour extern compart diazomethan apparatu place ml solut obtain ml methanol place mg diazald central reservoir add ml carbitol ml diethyl ether dissolv assembl apparatu half immers apparatu bath introduc syring ml cool potassium hydroxid solut central reservoir ensur yellow colour form format diazomethan persist yellow colour persist repeat methyl mg diazald apparatu remov bath minut left close ambient temperatur hour open apparatu react excess diazomethan ad drop solut formic acid ethyl acet transfer organ solut ml volumetr flask make volum hexan inject solut chromatograph methyl standard cool reagent apparatu hour extern compart diazomethan apparatu introduc ml solut ml ethyl acet ml methanol continu methyl inject result solut chromatograph ga chromatographi column yield resolut quot quot equal gt pic file quot quot gt retent time minut peak width half height millimetr chart speed millimetr minut ga chromatograph condit found suitabl column stainless steel length diamet mm support chromosorb waw siev analysi mesh stationari phase ov temperatur column injector detector carrier ga oxygen flee nitrogen pressur bar flow ml min persist yellow color excess diazomethan ensur complet methyl sampl calcul proportion coeffici hexachlorophen calcul respect chosen standard relat standard mixtur hexachlorophen kh proportion coeffici mass gram mixtur peak area chosen standard mass gram mixtur peak area gt pic file quot quot gt amount hexachlorophen sampl hexachlorophen kh proportion coeffici ah peak area chosen standard ms mass gram mixtur peak area mass gram sampl hexachlorophen sampl gt pic file quot quot gt repeat content hexachlorophen differ result determin carri parallel sampl exce absolut quantit determin tosylchloramid sodium inn chloramin scope field applic method relat quantit thin layer chromatograph determin tosylchloramid sodium chloramin cosmet product norm iso definit chloramin content sampl determin method express percentag mass principl chloramin complet hydrolyz toluenesulphonamid boil hydrochlor acid amount toluenesulphonamid form determin photo densitometr thin layer chromatographi reagent reagent analyt puriti tosylchloramid sodium chloramin standard solut toluenesulphonamid mg toluenesulphonamid ml ethanol gt pic file quot quot gt diethyl ether ethanol develop solvent butanol ethanol water chloroform aceton readi prepar thin layer chromatographi plate silica gel fluoresc indic potassium permangan hydrochlor acid sprai reagent toluidin solut ethanol apparatu normal laboratori apparatu usual thin layer chromatographi equip photodensitomet procedur hydrolysi weigh accur ml round bottom flask approxim sampl add ml water ml hydrochlor acid boil hour reflux condens immedi transfer hot suspens water ml graduat flask cool make mark water centrifug rpm minut pass supernat liquid filter extract ml filtrat extract time ml diethyl ether dry ether phase collect ml graduat flask make diethyl ether ml dri ether extract evapor dryness nitrogen stream redissolv residu ml ethanol thin layer chromatographi spot ethanol residu thin layer chromatographi plate time manner appli standard solut toluenesulphonamid develop approxim mm develop solvent complet evapor develop solvent place plate minut atmospher chlorin vapour produc pour ml hydrochlor acid potassium permangan close vessel remov excess chlorin heat plate minut sprai plate reagent measur approxim hour measur violet spot mean photo densitomet nm plot calibr curv plot maximum peak height valu ascertain toluenesulphonamid spot quantiti toluenesulphonamid toluenesulphonamid spot note method control solut chloramin treat sampl calcul chloramin content sampl express percentag mass calcul gt pic file quot quot gt toluenesulphonamid chloramin convers factor quantiti toluenesulphonamid sampl read calibr curv mass gram sampl repeat chloramin content differ result determin carri parallel sampl exce absolut determin total fluorin dental cream scope field applic method design determin total fluorin dental cream suitabl level excess definit fluorin content sampl determin method express percentag mass principl determin carri ga chromatographi fluorin fluorin compound convert triethylfluorosilan tef direct reaction chlorotriethylsilan tec acid solut simultan extract xylen cyclohexan intern standard reagent reagent analyt puriti sodium fluorid dri constant mass water doubl distil equival qualiti gt pic file quot quot gt cyclohexan ch xylen peak chromatogram prior solvent peak chromatograph condit sampl purifi distil norm iso chlorotriethylsilan tec merck equival fluorin standard solut stock solut mg ml weigh accur mg sodium fluorid dissolv water quantit transfer solut ml volumetr flask dilut mark water mix dilut stock solut mg ml transfer pipett ml stock solut ml volumetr flask dilut mark water mix intern standard solut mix ml cyclohexan ml xylen chlorotriethylsilan intern standard solut transfer pipett ml tec ml intern standard solut ml volumetr flask dilut xylen mark mix prepar fresh daili perchlor acid perchlor acid water apparatu standard laboratori equip ga chromatograph fit flame ioniz detector vortex swirl mixer equival bühler shaker type smb equival volumetr flask ml made polypropylen centrifug tube glass ml teflon line screw cap sovirel type equival clean tube screw cap leach hour perchlor acid subsequ rins water final dry pipett adjust deliv volum dispos plastic tip distil apparatu fit ball schneider column equival vigreux column procedur sampl analysi select dental cream tube previous open cut open tube remov content transfer plastic contain mix store condit avoid deterior weigh accur mg sampl centrifug tube add ml water homogen add ml xylen add dropwis ml hydrochlor acid homogen add pipett ml chlorotriethylsilan intern standard solut centrifug tube close tube screw cap mix minut shaker set stroke minut centrifug minut speed produc clear separ phase uncap tube withdraw organ layer inject organ phase column ga chromatograph remark take minut compon elut repeat inject calcul averag peak area ratio atef ach read amount fluorin milligram calibr graph calcul total fluorin content sampl cent mass fluorin paragraph chromatograph condit column stainless steel length diamet mm support gaschrom mesh stationari phase silicon oil dc equival condit column overnight carrier ga flow ml nitrogen minut repeat night fourth inject recondit column heat minut temperatur column injector detector ga flow carrier ml nitrogen minut calibr graph place pipett seri centrifug tube ml dilut fluorid standard solut make volum tube ml water proce inclus inject organ phase column ga chromatograph repeat inject calcul averag peak ratio atef ach plot calibr graph correl mass fluorin milligram standard solut peak area ratio atef ach measur connect point graph fit straight line calcul regress analysi calcul concentr total fluorin content sampl cent mass fluorin gt pic file quot quot gt test portion milligram amount milligram read calibr graph repeat fluorin content differ result determin carri parallel sampl exce absolut identif determin organomercuri compound scope field applic method identifi determin organomercuri deriv emploi preserv cosmet product ey applic thiomers inn sodium ethylmercuriothio benzoat phenylmercuri salt identif principl organomercuri compound complex diphenyl thiocarbazon extract dithizon carbon tetrachlorid silica gel thin layer chromatographi carri spot dithizon orang colour reagent reagent analyt puriti sulphur acid norm iso diphenyl thiocarbazon dithizon mg ml carbon tetrachlorid nitrogen carbon tetrachlorid develop solvent hexan aceton standard solut water sodium ethylmercuriothio benzoat ethylmercuri chlorid methylmercuri chlorid phenylmercuri nitrat phenylmercuri acet mercuri dichlorid mercuri di acet readi prepar silica gel plate merck equival sodium chlorid apparatu normal laboratori equip normal tlc apparatu phase separ filter procedur extract dilut sampl centrifug tube titrat ml distil water obtain maximum dispers warm water bath add sodium chlorid shake cool centrifug minut rev min order separ greater part solid liquid filter separ funnel add ml sulphur acid solut extract time ml dithizon solut organ phase remain green filter organ phase sequenti phase separ filter evapor dryness stream nitrogen dissolv ml carbon tetrachlorid appli solut immedi separ identif appli immedi carbon tetrachlorid solut obtain silica gel plate treat simultan ml standard solut appli solut obtain plate place plate solvent rise mm organomercuri compound colour spot colour stabl provid plate cover glass plate immedi solvent evapor rf valu obtain gt pic file quot quot gt determin definit content organomercuri compound determin method express percentag mass mercuri sampl principl method consist measur quantiti total mercuri present made mercuri inorgan state present identifi organomercuri deriv contain sampl miner mercuri liber measur flameless atom absorpt reagent reagent analyt puriti gt pic file quot quot gt gt pic file quot quot gt apparatu normal laboratori equip apparatu flameless atom absorpt mercuri determin cold vapour techniqu includ glasswar path length cell mm procedur normal precaut trace mercuri analysi breakdown weigh accur mg sampl add ml nitric acid leav digest hour airtight flask water bath shake regular interv time carri blank test reagent cool add ml sulphur acid return water bath minut place flask ic bath add carefulli ml water ad ml aliquot potassium permangan solut solut remain colour return water bath minut add ml dipotassium peroxodisulph solut continu warm water bath minut cool transfer content flask ml standard flask rins flask ml hydroxylammonium chlorid rins time ml water solut complet decolor make mark water determin place ml test solut glass vessel cold vapour mercuri determin dilut ml water subsequ ml sulphur acid ml tin dichlorid solut mix addit wait second reduc ionic mercuri metal state read place palladium dichlorid impregn glass wool mercuri reduct vessel flow cell instrument repeat record read read miner incomplet analysi repeat calcul mass milligram test sampl quantiti mercuri read instrument quantiti mercuri express mercuri percentag mass calcul formula gt pic file quot quot gt note improv miner start dilut sampl absorpt mercuri substrat suspect quantit determin method standard addit repeat case mercuri concentr differ result determin carri parallel sampl exce absolut determin alkali alkalin earth sulphid scope field applic method describ determin sulphid present cosmet product presenc thiol reduc agent includ sulphit interfer definit concentr sulphid determin method express percentag sulphur mass principl acidif medium hydrogen sulphid entrain stream nitrogen fix form cadmium sulphid filter rins determin iodometri reagent reagent analyt puriti norm iso gt pic file quot quot gt apparatu usual laboratori equip ml round bottom flask standard ground glass neck ml conic flask ground glass neck fit devic compris dip tube side outlet tube releas entrain ga long stem tunnel procedur entrain sulphid packag previous open weigh accur mass express gram product mg sulphid ion round bottom flask add ml water drop anti foam liquid transfer ml solut conic flask fit drop funnel dip tube outlet tube round bottom flask connect outlet tube conic flask set seri mean pvc tube nb entrain apparatu pass leak tight test simul test condit replac product determin ml sulphid solut prepar quot mg quot sulphid iodometr determin quot quot number milligram sulphid found end oper differ quantiti quot quot quantiti quot quot exce pass nitrogen minut rate bubbl order expel air contain round bottom flask heat round bottom flask stop nitrogen stream add ml hydrochlor acid drop drop turn nitrogen stream acid transfer leav minimum liquid seal prevent leakag hydrogen sulphid ceas heat minut flask cool continu pass nitrogen stream half hour titrat filter cadmium sulphid long stem funnel rins conic flask ammonia solut pour filter rins distil water water wash precipit retain filter complet wash precipit ml water place paper filter conic flask contain precipit add ml iodin solut approxim ml hydrochlor acid ml distil water determin excess iodin sodium thiosulph solut calcul sulphid content sampl express sulphur percentag mass calcul formula gt pic file quot quot gt number millilitr iodin standard solut molar solut number millilitr sodium thiosulph standard solut molar solut mass gram test sampl repeat sulphid content differ result determin carri parallel sampl exce absolut norm iso