commiss decis march fix criteria test poultri slaughter origin surveil zone newcastl diseas applic articl council direct eec text eea relev ec commiss european commun regard treati establish european commun regard council direct eec june anim health condit govern intra commun trade import countri fresh poultri meat amend direct ec articl thereof applic provis articl direct eec determin methodolog perform virolog test newcastl diseas regard provid detail sampl procedur procedur carri test interpret test result scientif veterinari committe consult deliv report decemb measur provid decis accord opinion stand veterinari committe adopt decis articl applic articl direct eec virolog sampl test detect newcastl diseas viru compli requir annex articl decis appli effect april articl decis address member state brussel march commiss franz fischler member commiss annex sampl leat sampl compris cloacal swab tracheal swab flock bird sampl sampl dai slaughter transport chill frozen nation newcastl diseas laboratori test treatment sampl sampl type pool swab suffici antibiot medium ensur full immers agit left hour ambient temperatur longer period clarifi centrifug minut antibiot medium typic cloacal swab unit ml penicillin mg ml streptomycin mg ml gentamycin unit ml mycostatin phosphat buffer salin ph ìg ml oxytetracyclin ad antibiot concentr reduc fold tracheal swab imper make medium ph check addit antibiot adjust viru isol embryon fowl egg clarifi supernat fluid inocul ml amount allanto caviti minimum embryon fowl egg incub dai ideal egg obtain specif pathogen free flock impractic accept egg obtain flock shown free antibodi newcastl diseas viru inocul egg held candl daili egg dead dy embryo aris remain egg dai inocul chill allanto amniot fluid test haemagglutin activ interpret test regard neg haemagglutin activ detect viru isol isol newcastl diseas viru mean flock treat suspect flock subject requir articl council direct eec viru prove vaccin origin sampl test repeat