commiss direct ec juli lai sampl method method analysi offici control dioxin determin dioxin pcb foodstuff text eea relev commiss european commun regard treati establish european commun regard council direct eec decemb introduct commun method sampl analysi monitor foodstuff intend human consumpt articl thereof commiss regul ec amend regul ec amend council regul ec establish maximum limit dioxin furan foodstuff council direct eec june offici control foodstuff lai gener principl perform control foodstuff council direct eec octob subject addit measur offici control foodstuff introduc system qualiti standard laboratori entrust member state offici control foodstuff direct eec fix gener criteria method sampl analysi case lai specif criteria requir method analysi compli order ensur laboratori method analysi compar level perform provis sampl method analysi drawn basi present knowledg adapt account advanc scientif technolog knowledg provis laid direct relat sampl analysi dioxin dioxin pcb implement regul ec affect sampl strategi sampl level frequenc annex iii iv council direct ec april measur monitor substanc residu thereof live anim anim product repeal direct eec eec decis eec eec affect target criteria sampl laid commiss decis ec februari lai detail rule offici sampl monitor substanc residu thereof live anim anim product activ approach pursu order obtain comprehens reliabl data presenc dioxin pcb foodstuff requir laid method analysi determin dioxin pcb foodstuff screen method analysi proven wide accept valid high throughput select sampl signific level dioxin level dioxin sampl determin confirmatori method analysi establish strict requir confirmatori method analysi minimum requir screen method measur provid direct accord opinion stand committe food chain anim health adopt direct articl member state ensur sampl offici control level dioxin furan determin level dioxin pcb foodstuff carri accord method annex articl member state ensur sampl prepar method analys offici control level dioxin furan determin level dioxin pcb foodstuff compli criteria annex ii articl member state bring forc law regul administr provis compli direct februari latest forthwith inform commiss thereof member state adopt provis refer direct accompani refer occas offici public member state determin refer made articl direct enter forc dai public offici journal european commun articl direct address member state brussel juli commiss david byrn member commiss oj oj oj oj oj oj oj oj annex method sampl offici control level dioxin pcdd pcdf determin dioxin pcb foodstuff purpos scope sampl intend offici control level dioxin pcdd pcdf content determin content dioxin pcb foodstuff method aggreg sampl obtain consid repres lot sublot complianc maximum level laid commiss regul ec set maximum level contamin foodstuff establish basi level determin laboratori sampl definit lot identifi quantiti food deliv time determin offici common characterist origin varieti type pack packer consignor mark case fish fisheri product size fish compar sublot design part larg lot order appli sampl method design part sublot physic separ identifi increment sampl quantiti materi singl place lot sublot aggreg sampl combin total increment sampl lot sublot laboratori sampl repres part quantiti aggreg sampl intend laboratori gener provis personnel sampl perform authoris qualifi person member state materi sampl lot examin sampl separ precaut sampl prepar laboratori sampl precaut avoid affect content dioxin dioxin pcb advers affect analyt determin make aggreg sampl unrepres increment sampl practic increment sampl place distribut lot sublot departur procedur record record provid prepar aggreg sampl aggreg sampl made unit increment sampl kg practic singl packag sampl subdivis aggreg sampl laboratori sampl enforc defenc refere purpos laboratori sampl enforc trade defenc refere purpos homogenis aggreg sampl conflict member state regul sampl size laboratori sampl enforc suffici duplic analys packag transmiss aggreg laboratori sampl aggreg laboratori sampl clean inert contain offer adequ protect contamin loss analyt adsorpt intern wall contain damag transit precaut avoid chang composit aggreg laboratori sampl aris transport storag seal label aggreg laboratori sampl sampl offici seal place sampl identifi member state regul record sampl permit lot identifi unambigu give date place sampl addit inform assist analyst sampl plan sampl method appli ensur aggreg sampl repres lot control number increment sampl case milk oil homogen distribut contamin question assum lot suffici increment sampl lot form aggreg sampl refer lot number product minimum number increment sampl lot tabl aggreg sampl unit increment sampl kg point increment sampl similar weight weight increment sampl gram weight increment sampl depend size particl lot departur procedur record record provid accord provis commiss decis ec octob fix level frequenc sampl provid council direct ec monitor substanc residu thereof anim product sampl size hen egg egg bulk lot lot consist individu packag tabl tabl minimum number increment sampl lot gt tabl gt lot consist individu packag number packag form aggreg sampl tabl tabl number packag increment sampl form aggreg sampl lot consist individu packag gt tabl gt complianc lot sublot specif control laboratori analys laboratori sampl enforc duplic analysi case obtain result analysi maximum level calcul result lot accept result analysi maximum level duplic analysi conform respect maximum level laid regul ec tabl tef human risk assess base conclus world health organis meet stockholm sweden june van den berg al toxic equival factor tef pcb pcdd pcdf human wildlif environment health perspect gt tabl gt abbrevi tetra pe penta hx hexa hp hepta octa cdd chlorodibenzodioxin cdf chlorodibenzofuran cb chlorobiphenyl oj annex ii sampl prepar requir method analysi offici control level dioxin pcdd pcdf determin dioxin pcb foodstuff object field applic requir appli foodstuff analys offici control level dioxin polychlorin dibenzo dioxin pcdd polychlorin dibenzofuran pcdf determin dioxin pcb monitor presenc dioxin foodstuff perform strategi involv screen method order select sampl level dioxin dioxin pcb exce level interest concentr dioxin sampl signific level determin confirm confirmatori method screen method method detect presenc dioxin dioxin pcb level interest method capac high sampl throughput sift larg number sampl potenti posit specif design avoid fals neg confirmatori method method provid full complementari inform enabl dioxin dioxin pcb identifi quantifi unequivoc level interest background environment biolog sampl includ sampl foodstuff gener complex mixtur dioxin congen concept toxic equival factor tef develop facilit risk assess tef establish express concentr mixtur substitut pcdd pcdf recent ortho mono ortho chlorin substitut pcb possess dioxin activ toxic equival teq tcdd annex footnot concentr individu substanc sampl multipli respect tef subsequ sum give total concentr dioxin compound express teq concept quot upperbound quot requir limit quantif contribut quantifi congen teq concept quot lowerbound quot requir contribut quantifi congen teq concept quot mediumbound quot requir half limit quantif calcul contribut quantifi congen teq qualiti assur requir compli sampl prepar measur avoid cross contamin stage sampl analysi procedur sampl store transport glass aluminium polypropylen polyethylen contain trace paper dust remov sampl contain glasswar rins solvent previous control presenc dioxin sampl storag transport perform maintain integr foodstuff sampl relev fine grind mix laboratori sampl process demonstr achiev complet homogenis ground pass mm siev sampl dri grind moistur content high perform blank analysi carri entir analyt procedur omit sampl sampl weight extract suffici fulfil requir respect sensit satisfactori specif sampl prepar procedur product consider procedur valid internation accept guidelin requir laboratori laboratori demonstr perform method rang level interest level interest accept coeffici variat repeat analysi detail accept criteria point limit quantif confirmatori method rang level interest make accept coeffici variat met rang level interest regular blank control spike experi analysi control sampl prefer certifi refer materi perform intern qualiti control measur success particip interlaboratori studi assess laboratori profici prove compet specif analys success particip interlaboratori studi soil sewag sampl necessarili prove compet field food feedingstuff sampl present lower contamin level continu particip interlaboratori studi determin dioxin dioxin pcb relev feed food matric mandatori accord provis direct eec laboratori accredit recognis bodi oper accord iso guid ensur appli analyt qualiti assur laboratori accredit iso iec standard requir met analyt procedur dioxin dioxin pcb basic requir accept analyt procedur high sensit low limit detect pcdd pcdf detect quantiti picogram teq rang extrem toxic compound pcb occur higher level pcdd pcdf pcb congen sensit nanogram rang suffici measur toxic dioxin pcb congen ortho substitut congen sensit reach pcdd pcdf high select specif distinct requir pcdd pcdf dioxin pcb multitud coextract possibl interf compound present concentr order magnitud higher analyt interest ga chromatographi mass spectrometri gc ms method differenti congen toxic seventeen substitut pcdd pcdf dioxin pcb congen bioassai determin teq valu select sum pcdd pcdf dioxin pcb high accuraci trueness precis determin provid valid estim true concentr sampl high accuraci accuraci measur close agreement result measur true assign measur avoid reject sampl analysi result basi poor reliabl estim teq accuraci express trueness differ measur analyt certifi materi certifi express percentag precis precis calcul standard deviat includ repeat reproduc close agreement result obtain appli experiment procedur time prescrib condit screen method compris bioassai gc ms method confirmatori method high resolut ga chromatographi high resolut mass spectrometri hrgc hrm method criteria compli total teq gt tabl gt specif requir gc ms method compli screen confirmatori purpos addit label chlorin substitut intern pcdd standard label intern dioxin pcb standard dioxin pcb determin carri begin start analyt method prior extract order valid analyt procedur congen tetra octa chlorin homolog group pcdd congen homolog group dioxin pcb dioxin pcb determin ad altern congen mass spectrometr select ion record function monitor pcdd dioxin pcb clear prefer case confirmatori method label substitut intern pcdd standard label intern dioxin pcb standard dioxin pcb determin rel respons factor determin congen label analogu ad calibr solut foodstuff plant origin foodstuff anim origin fat addit intern standard mandatori prior extract foodstuff anim origin fat intern standard ad extract fat extract valid extract effici carri depend stage intern standard introduc result report product fat basi prior gc ms analysi recoveri surrog standard ad control recoveri confirmatori method recoveri individu intern standard rang lower higher recoveri individu congen hepta octa chlorin dibenzodioxin dibenzofuran accept condit contribut teq exce total teq base pcdd screen method recoveri rang separ dioxin interf chlorin compound pcb chlorin diphenyl ether carri suitabl chromatograph techniqu prefer florisil alumina carbon column gaschromatograph separ isom suffici amp lt peak peak hxcdf hxcdf determin perform epa method revis tetra octa chlorin dioxin furan isotop dilut hrgc hrm equival perform criteria differ upperbound level lower bound level exce foodstuff dioxin contamin pg teq fat base pcdd pcdf foodstuff low fat content requir contamin level pg teq product appli lower contamin level pg teq product differ upperbound lowerbound level rang screen method analysi introduct analyt approach perform screen method pure screen approach quantit approach screen approach respons sampl compar refer sampl level interest sampl respons refer declar neg higher respons suspect posit requir blank refer sampl includ test seri extract test time ident condit refer sampl show elev respons comparison blank extra refer sampl level interest includ demonstr proper perform test rang interest control level interest test matric suitabl refer sampl demonstr preferenti includ sampl shown hrgc hrm teq level refer sampl blank spike level intern standard bioassai test repeat import obtain inform standard deviat test seri coeffici variat bioassai target compound interfer maximum toler blank level defin quantit approach quantit approach requir standard dilut seri duplic triplic clean measur blank recoveri control result express teq assum compound respons signal correspond teq principl perform tcdd dioxin furan standard mixtur produc calibr curv calcul teq level extract sampl subsequ correct teq level calcul blank sampl account impur solvent chemic recoveri calcul teq level qualiti control sampl level interest essenti note part appar recoveri loss due matrix effect differ tef valu bioassai offici tef valu set requir method analysi screen gc ms method analysi bioassai screen gc ms method requir laid point cell base bioassai specif requir laid point kit base bioassai point inform number fals posit fals neg result larg set sampl maximum level action level comparison teq content determin confirmatori method analysi actual fals neg rate rate fals posit sampl low make screen tool advantag posit result confirm confirmatori method analysi hrgc hrm addit sampl wide teq rang confirm hrgc hrm approxim neg sampl inform correspond bioassai hrgc hrm result made specif requir cell base bioassai perform bioassai test run requir seri refer concentr tcdd dioxin furan mixtur full dose respons curv amp gt screen purpos expand low level curv analys low level sampl tcdd refer concentr limit quantif qualiti control sheet outcom bioassai constant time period altern rel respons refer sampl comparison tcdd calibr line respons cell depend factor qualiti control qc chart type refer materi record check make outcom accord state guidelin quantit calcul induct sampl dilut linear portion respons curv sampl linear portion respons curv dilut test dilut time recommend test percent standard deviat triplic determin sampl dilut independ experi limit detect set standard deviat solvent blank background respons approach appli respons background induct factor solvent blank calcul calibr curv dai limit quantif set standard deviat solvent blank background respons appli respons background induct factor solvent blank calcul calibr curv dai specif requir kit base bioassai manufactur instruct sampl prepar analys test kit expir date materi compon design kit test kit rang storag temperatur oper temperatur limit detect immunoassai determin standard deviat base replic analysi blank divid slope linear regress equat refer standard test laboratori make respons standard accept rang report result analyt procedur make analyt result level individu pcdd pcb congen report lowerbound upperbound mediumbound order includ maximum inform report result enabl interpret result specif requir report includ lipid content sampl method lipid extract recoveri individu intern standard made case recoveri rang mention point case maximum level exceed case request evid submit commerci kit base bioassai suffici sensit reliabl screen presenc dioxin requir level sampl foodstuff feedingstuff