commiss decis juli establish diagnost procedur sampl method criteria evalu result laboratori test confirm differenti diagnosi swine vesicular diseas notifi document number text eea relev ec commiss european commun regard treati establish european commun regard council direct eec decemb introduc gener commun measur control anim diseas specif measur relat swine vesicular diseas amend act access austria finland sweden annex ii paragraph thereof lai commun level diagnost procedur sampl method criteria evalu result laboratori test confirm swine vesicular diseas rapid differenti foot mouth diseas order improv control diseas ensur annex iii council direct eec lai function duti commun refer laboratori swine vesicular diseas order coordin consult commiss method emploi member state diagnos diseas function duti includ organis period compar test suppli standard reagent commun level laboratori test recent develop ensur swine vesicular diseas quickli diagnos distinguish foot mouth diseas result recent compar test perform commun level suggest reliabl test develop detect antigen genom swine vesicular diseas viru test successfulli supplement viru isol test virolog diagnosi swine vesicular diseas experi gain control swine vesicular diseas recent year result identif suitabl sampl procedur criteria evalu result laboratori test proper diagnosi diseas situat opinion recommend scientif committe anim health welfar swine vesicular diseas account measur provid decis accord opinion stand veterinari committe adopt decis articl member state ensur confirm swine vesicular diseas differenti diagnosi foot mouth diseas base detect clinic sign diseas detect viru antigen genom sampl epithelium tissu vesicular fluid faec demonstr specif antibodi respons serum sampl accord procedur sampl method criteria evalu result laboratori test laid manual annex decis nation diagnost laboratori refer annex ii direct eec appli modif laboratori test refer manual annex decis test provid equal sensit specif demonstr sensit specif modifi test evalu framework period compar test organis commun refer laboratori swine vesicular diseas articl decis appli octob articl decis address member state brussel juli commiss david byrn member commiss oj annex manual diagnost procedur sampl method criteria evalu result laboratori test confirm differenti diagnosi swine vesicular diseas chapter introduct object definit manual guidelin minimum requir diagnost procedur sampl method criteria evalu result laboratori test proper diagnosi swine vesicular diseas emphasi differenti diagnosi foot mouth diseas integr provis annex ii direct eec paragraph annex princip direct author respons control swine vesicular diseas emphasi principl applic laboratori test evalu result detail laboratori techniqu purpos manual definit appli quot seroposit pig quot mean pig serum antibodi titr equal greater swine vesicular diseas refer serum refer chapter viru neutralis test nation laboratori quot singleton reactor quot mean singl seroposit pig hold yield posit result serolog test swine vesicular diseas histori contact swine vesicular diseas viru evid spread infect contact pig seroposit pig confirm singleton reactor condit refer chapter viii fulfil quot contact pig quot mean pig direct contact direct contact dai seroposit pig pig suspect infect swine vesicular diseas viru contact pig pen adjac pen possibl pig pig contact pen chapter ii guidelin check pig show clinic sign swine vesicular diseas member state ensur presenc swine vesicular diseas viru suspect hold statist signific number pig check offici veterinarian quickli detect clinic sign diseas refer chapter ix member state ensur pig show clinic sign suggest swine vesicular diseas foot mouth diseas differenti diagnosi mean sampl laboratori investig carri quickli accord provis refer chapter iv vii viii manual chapter iii gener procedur sampl transport sampl person enter leav pig hold suspicion swine vesicular diseas observ strictest hygien measur reduc risk contamin spread viru pig sampl uniqu mark identifi eventu sampl recommend locat pig sampl hold record uniqu identif mark suspect pig sampl sampl laboratori accompani form includ detail histori pig sampl clinic sign observ vesicular condit pig foot mouth diseas special precaut safe packag suspect sampl precaut design prevent breakag leakag contain risk contamin import ensur specimen arriv satisfactori state wet ic put insid packag escap water prevent contain sampl suspect swine vesicular diseas viru open leav infect premis arriv laboratori sampl suspect swine vesicular diseas viru analys laboratori authoris handl foot mouth diseas viru diagnost purpos accord commun legisl control foot mouth diseas foot mouth diseas rule sampl transport anticip transport time recipi laboratori hour maintain temperatur sampl destin commun refer laboratori member state unit kingdom permit method transport airfreight london heathrow london gatwick airport shipment laboratori inform fax mail detail airlin flight date expect time arriv airwaybil number parcel locat arriv parcel address institut anim health pirbright laboratori commun refer laboratori swine vesicular diseas ash road pirbright woke surrei gu onf unit kingdom uk inform includ label quot anim patholog materi commerci perish fragil collect airport addresse open laboratori quot authoris collect consign airport made personnel commun refer laboratori special gener import permit issu purpos unit kingdom ministri agricultur food fisheri stand arrang separ licenc requir import hand carriag suspect vesicular materi unit kingdom unauthoris personnel permit courier compani transport sampl nation laboratori accord instruct laid compet author member state chapter iv sampl procedur hold clinic suspect pig presenc swine vesicular diseas viru hold suspect clinic sign diseas observ sampl repres group pig show sign collect diseas confirm differenti diagnosi foot mouth diseas hold prefer sampl diagnosi epithelium vesicular fluid unruptur freshli ruptur vesicl collect pig show typic sign diseas swine vesicular diseas viru antigen genom detect recommend pig sampl fresh epitheli tissu vesicular fluid suffici quantiti sampl collect blood sampl suspect pig contact pig serolog test faecal sampl suspect pig floor pen adjac pen virolog test sampl collect transport accord procedur epithelium sampl vesicular fluid epithelium tissu unruptur recent ruptur vesicl collect recommend pig sedat sampl collect avoid injuri personnel pig welfar transport nation laboratori carri immedi hour epitheli sampl transport dry refriger time exce hour sampl small volum transport medium consist equal amount glycerol phosphat buffer equival buffer hepe ph maintain optim rang foot mouth diseas viru surviv ph transport medium antibiot addit anti microbi activ suitabl antibiot concentr ml final penicillin iu ii neomycin sulphat iu iii polymyxin sulphat iu iv mycostatin iu vesicular fluid collect unruptur vesicl undilut separ contain blood sampl blood sampl collect serolog virolog test gener collect pig suspect recov clinic subclin infect antibodi detect epithelium vesicular fluid faecal sampl pig show clinic sign diseas suitabl viru detect blood sampl recommend blood sampl vacutain anticoagul vacutain transport unopen faecal sampl faecal sampl floor premis suspect contain pig infect swine vesicular diseas faecal swab faecal sampl suspect live pig strong leak proof contain contain suspect sampl disinfect transport laboratori suitabl disinfect sodium hydroxid dilut formalin dilut solut formalin minimum formaldehyd sodium hypochlorit chlorin disinfect handl care chapter sampl procedur swine vesicular diseas serosurveil sero surveil carri purpos surveil hold evid suspicion diseas present surveil slaughterhous market collect centr similar place routin serolog sampl discriminatori surveil pig receiv member state import hold blood sampl collect serolog test pig accord provis laid monitor erad programm plan approv framework decis eec direct eec absenc provis procedur establish compet author member state sero surveil carri purpos surveil hold locat protect surveil zone establish confirm diseas outbreak accord annex ii direct eec surveil hold refer articl direct eec blood sampl collect serolog test pig accord scheme case breed hold randomis sampl procedur carri detect preval seroconvers confid case hold fatten pig sampl procedur ensur total number sampl collect equal number requir detect preval confid case sampl random select pen case mix breed fatten hold group pig live separ premis sampl detect preval seroconvers confid chapter vi action sampl procedur case find seroposit pig case singl seroposit pig detect hold surveil refer chapter compet author ensur appli measur refer articl direct eec appli hold check carri hold accord provis refer chapter ii blood sampl collect serolog test suspect pig contact pig live adjac pen suspect pig pig sampl detect preval seroconvers confid pen compet author decid lift measur refer epidemiolog enquiri carri accord articl direct eec suggest swine vesicular diseas introduc hold clinic sign swine vesicular diseas detect hold hold locat surveil restrict zone establish confirm outbreak diseas subject restrict appli relat confirm outbreak diseas provid pig move hold intra commun trade pig hold question move slaughterhous slaughter hold pig move intra commun trade result check serolog test swine vesicular diseas rule check serolog test carri accord give neg result previous posit pig confirm posit singleton reactor swine vesicular diseas rule measur refer lift hold locat protect surveil zone establish outbreak diseas diseas erad measur stai forc accord annex ii direct eec seroposit pig present hold swine vesicular diseas confirm condit laid annex ii direct eec fulfil confirm presenc diseas sampl hold accord sampl procedur refer case seroposit pig detect hold sampl serolog test refer chapter condit laid annex ii direct eec fulfil confirm swine vesicular diseas compet author ensur provis refer articl direct eec appli continu appli check hold carri accord provis refer chapter ii blood sampl serolog test collect seroposit pig contact pig accord blood sampl serolog test collect pig build hold accord procedur refer chapter suffici number faecal sampl collect virolog test seroposit pig floor pen seroposit pig adjac pen random select pen build hold faecal sampl collect accord indent examin case sampl neg result serolog test suggest swine vesicular diseas viru spread build faecal sampl collect accord indent examin check test condit laid annex ii direct eec fulfil confirm presenc swine vesicular diseas seroposit pig kill slaughter accord provis refer annex ii direct eec pig found seroposit addit found seroposit previou sampl provis procedur laid appli mutati mutandi prejudic measur refer articl direct eec case seroposit pig detect surveil activ refer chapter compet author ensur feasibl check includ collect sampl carri confirm rule swine vesicular diseas place pig detect take account local situat measur refer articl direct eec appli hold origin pig check carri hold origin pig accord provis refer chapter ii blood sampl collect serolog test pig hold origin seroposit pig accord provis refer chapter compet author decid lift measur refer epidemiolog enquiri carri accord articl council direct eec suggest swine vesicular diseas introduc hold clinic sign swine vesicular diseas detect hold hold locat surveil restrict zone establish confirm outbreak diseas subject restrict appli relat confirm outbreak diseas provid pig move hold intra commun trade pig move hold slaughterhous slaugher hold pig move intra commun trade result check serolog test carri place seroposit pig detect hold origin swine vesicular diseas rule chapter vii principl applic virolog test evalu result detect viru antigen indirect sandwich elisa replac complement fixat test method choic detect swine vesicular diseas viral antigen test foot mouth diseas diagnosi test diseas perform time foot mouth diseas rule recommend sampl epithelium fluid vesicular lesion swine vesicular diseas foot mouth diseas virus present high titer acut infect pig detect hour duplic row multiwel elisa plate coat rabbit antiserum swine vesicular diseas viru serotyp foot mouth diseas viru trap sera test sampl suspens ad row control includ homolog guinea pig detect serum ad respect row stage rabbit anti guinea pig serum conjug enzym hors radish peroxidas extens wash carri stage remov unbound reagent posit reaction colour reaction addit chromogen substrat strong posit reaction evid nake ey result read spectrophotometr case absorb read background posit reaction altern monoclon antibodi base elisa system select monoclon antibodi trap antibodi peroxidas conjug monoclon antibodi detect antibodi swine vesicular diseas antigen detect differenti diagnosi foot mouth diseas epithelium sampl vesicular fluid infect tissu cultur monoclon antibodi base elisa studi antigen variat strain swine vesicular diseas viru tissu cultur grown viral antigen trap rabbit hyperimmun anti serum swine vesicular diseas adsorb solid phase panel monoclon antibodi react bind monoclon antibodi field strain compar bind monoclon antibodi parent strain similar bind presenc epitop share parent field strain isol growth viru routin clarifi suspens sampl epithelium vesicular fluid faec suspect swine vesicular diseas viru inocul sensit cell cultur quantiti qualiti sampl vesicular lesion submit examin insuffici examin elisa growth viru tissu cultur amplifi viral antigen isol grow viru clarifi epitheli suspens inocul monolay cultur ib rs cell dilut epitheli suspens high low avoid interfer viru growth interferon releas interfer growth swine vesicular diseas viru viru isol antibiot ad mainten medium differenti diagnosi foot mouth diseas viru primari bovin thyroid cell babi hamster kidnei cell bhk inocul cytopath effect develop supernat fluid harvest posit cultur effect complet elisa viru identif neg cultur inocul fresh tissu cultur hour blind passag examin hour absenc cytopath effect blind passag sampl declar neg presenc live viru suspens faecal sampl process gener viru faec epithelium essenti absenc cytopath effect passag blind passag includ simultan inocul porcin cell line mention tissu cultur system prefer primari bovin thyroid cell guid vesicular sampl swine vesicular diseas viru foot mouth diseas viru grow cell porcin origin foot mouth diseas viru isol prolong histori transmiss pig preferenti grow porcin cell cultur system polymeras chain reaction pcr genom detect nucleic acid recognit method detect swine vesicular diseas viral genom clinic materi pcr establish relationship isol swine vesicular diseas viru determin nucleotid sequenc part genom techniqu pcr develop improv sensit diagnosi slightli revers transcriptas pcr procedur primer highli conserv region gene pcr techniqu rapid result hour detect genotyp swine vesicular diseas viru suffici sensit sampl collect case suspect clinic diseas clinic infect suspect sampl collect resolut clinic diseas process faecal sampl enhanc rt pcr techniqu nest rt pcr immun pcr elisa pcr elabor rna extract method produc detect system sensit consider rapid multipl passag tissu cultur sequenc approxim nucleotid gene code major structur protein vp group strain swine vesicular diseas viru sequenc homolog epidemiolog relat strain caus diseas region time evalu result virolog test detect antigen genom swine vesicular diseas viru mean elisa pcr diagnost viru isol viru isol consid refer test confirmatori test posit elisa pcr result detect clinic sign diseas detect seroposit pig direct epidemiolog connect confirm outbreak chapter viii principl applic serolog test evalu result viru neutral vn test quantit vn micro test swine vesicular diseas viru antibodi detect perform ib rs cell equival cell system flat bottom tissu cultur grade microtitr plate viru grown ib rs cell monolay store addit glycerol glycerol sera inactiv minut test elisa elisa detect antibodi monoclon antibodi base competit elisa serum sampl antibodi swine vesicular diseas viru bind select peroxidas conjug monoclon antibodi viru antigen inhibit elisa swine vesicular diseas viral antigen trap solid phase monoclon antibodi sera sampl incub dilut addit peroxidas conjug monoclon antibodi inhibit monoclon antibodi bind measur mean substrat chromogen indirect trap elisa isotyp specif monocolon antibodi detect swine igg igm specif swine vesicular diseas viru help assess time infect pig infect premis isotyp specif elisa viral antigen trap solid phase antigen catch antibodi serum sampl antibodi swine vesicular diseas viru detect anti pig igg anti pig igm monoclon antibodi conjug peroxidas bind measur mean substrat chromogen isotyp specif elisa distinguish singleton reactor true posit pig refer applic serolog test evalu result vn test elisa recommend serolog test chapter list refer sera commun refer laboratori order standardis serolog test carri commun vn test consid refer test disadvantag take dai complet requir tissu cultur facil elisa rapid easili standardis monoclon antibodi competit elisa reliabl swine vesicular diseas antibodi elisa date recommend screen test larg number sampl vn test confirmatori test detect posit sampl hold pig posit elisa neg vn test disregard presenc singleton reactor suspect singl seroposit pig detect criteria met clinic sign diseas hold relev histori clinic diseas hold histori contact outbreak diseas pig confirm singleton reactor follow test identifi seroposit pig sampl perform contact pig detect singleton reactor reveal seroconvers antibodi titr repeat sampl remain constant declin addit criteria principl consid confirm singleton reactor singleton reactor occur preval approxim pig sera singleton reactor gener profil low vn test antibodi titr borderlin posit monoclon antibodi base competit elisa exclus igm igg swine vesicular diseas isotyp specif elisa chapter ix clinic sign featur swine vesicular diseas swine vesicular diseas contagi diseas pig caus enteroviru picornavirida famili clinic mild sever vesicular condit depend strain viru involv rout dose infect husbandri condit pig addit stress factor transport mix pig temperatur extrem predispos develop clinic sign characteris mild fever vesicl coronari band bulb heel skin limb frequent snout lip tongu teat morbid rate high mortal low nil infect develop inappar mild form show transitori declin gener appear pig lead develop viru neutralis antibodi dai subclin mild natur diseas suspect serolog test diseas surveil export certif recent european outbreak swine vesicular diseas characteris sever clinic sign diagnosi frequent depend serolog clinic sign swine vesicular diseas indistinguish foot mouth diseas vesicular condit treat initi suspect foot mouth diseas differenti diagnosi obtain quickli incub period swine vesicular diseas individu pig dai transient fever occur clinic sign evid hold longer period vesicl develop coronari band typic junction heel affect coronari band result loss hoof rare vesicl snout dorsal surfac lip tongu teat shallow eros knee affect pig lame feed dai younger pig sever affect mortal due swine vesicular diseas rare contrast foot mouth diseas young stock nervou sign report unusu abort typic featur swine vesicular diseas cardiac failur due multifoc myocard featur foot mouth diseas encephalomyocard young piglet occur swine vesicular diseas recoveri complet week evid infect dark horizont line hoof growth temporarili interrupt affect pig excret viru nose mouth faec hour onset clinic sign viru produc dai infect viru excret nose mouth stop week viru isol faec dai infect report present month persist consider period time necrot tissu ruptur vesicl faec chapter swine vesicular diseas refer sera gt tabl gt oj oj posit elisa result presenc tcid tissu cultur infecti dose viru sampl small proport singleton reactor detect current serolog test swine vesicular diseas factor respons singleton reactor unknown serolog cross reactiv swine vesicular diseas viru aris due infect unidentifi picornaviru due specif factor present serum specif igg igg igm detect serum sampl swine vesicular diseas viru infect pig sera singleton reactor gener igm specif igg detect serum sampl pig infect swine vesicular diseas viru previou dai specif igg detect blood sampl recent infect pig reliabl distinguish singleton reactor immun respons switch igm igg product chapter ix footnot specif igm detect blood dai post infect disappear dai specif igg detect blood dai post infect year ig isotyp determin mean elisa chapter viii