commiss decis februari approv diagnost manual establish diagnost procedur sampl method criteria evalu laboratori test confirm classic swine fever notifi document number text eea relev ec commiss european commun regard treati establish european commun regard council direct ec octob commun measur control classic swine fever articl articl thereof lai commun level diagnost procedur sampl method criteria evalu result laboratori test confirm classic swine fever annex iv direct ec lai function duti commun refer laboratori classic swine fever order coordin consult commiss method emploi member state diagnos diseas function duti includ organis period compar test suppli standard reagent commun level classic swine fever viru consid hazard human health laboratori test recent develop ensur quick diagnosi classic swine fever experi gain control classic swine fever recent year result identif suitabl sampl procedur criteria evalu result laboratori test proper diagnosi diseas situat measur provid decis accord opinion stand veterinari committe adopt decis articl member state ensur confirm classic swine fever base detect clinic sign post mortem lesion diseas detect viru antigen genom sampl pig tissu organ blood excreta demonstr specif antibodi respons blood sampl accord procedur sampl method criteria evalu result laboratori test laid manual annex decis nation diagnost laboratori refer annex iii direct ec appli modif laboratori test refer manual annex decis test provid equal sensit specif demonstr sensit specif modifi test evalu framework period compar test organis commun refer laboratori classic swine fever articl annex iv council direct eec januari introduc commun measur control classic swine fever amend act access austria finland sweden repeal articl decis appli novemb articl decis address member state brussel februari commiss david byrn member commiss oj oj annex classic swine fever diagnost manual chapter introduct object definit order ensur uniform procedur diagnos classic swine fever manual guidelin minimum requir diagnost procedur sampl method criteria evalu result clinic post mortem examin laboratori test proper diagnosi classic swine fever establish minimum bio safeti requir qualiti standard observ classic swine fever diagnost laboratori transport sampl establish laboratori test diagnosi classic swine fever laboratori techniqu genet type classic swine fever viru isol manual princip direct author respons control classic swine fever emphasi principl applic laboratori test evalu result detail laboratori techniqu purpos manual addit definit refer articl direct ec definit appli quot suspect hold quot mean pig hold pig suspect infect classic swine fever viru contact hold defin articl direct ec quot singleton reactor quot mean pig yield posit result serolog test classic swine fever histori contact classic swine fever viru evid spread infect contact pig quot epidemiolog unit quot quot unit quot mean build place land nearbi group pig hold frequent direct indirect contact meantim separ pig hold quot contact pig quot mean pig live hold direct contact pig suspect infect classic swine fever viru dai chapter ii descript classic swine fever emphasi differenti diagnosi introduct classic swine fever caus envelop rna viru belong genu pestiviru flavivirida famili viru relat rumin pestivirus caus bovin viral diarrhoea bvdv border diseas bdv relationship diagnost consequ cross reaction occur lead fals posit result laboratori test classic swine fever viru stabl moist excret infect pig pig carcass fresh pig meat pig meat product readili inactiv deterg lipid solvent proteas common disinfect main natur rout infect oro nasal direct indirect contact infect pig feed viru contamin feed area high densiti pig spread viru easili occur neighbour pig hold diseas transmiss semen infect boar occur incub period individu anim week ten dai field condit clinic symptom evid hold week viru introduct adult breed pig mild strain viru concern clinic sign classic swine fever extrem variabl confus diseas sever symptom depend ag anim viru virul young anim affect sever older anim older breed pig infect mild clinic acut chronic prenat form classic swine fever distinguish acut form weaner fatten pig displai acut form classic swine fever initi sign anorexia lethargi fever conjunct swollen lymph node respiratori sign constip diarrhoea typic haemorrhag skin observ ear tail abdomen side limb week infect death neurolog sign frequent stagger hind limb gait coordin movement convuls constant find fever higher adult pig fever exce classic swine fever viru sever leukopenia immunosuppress lead enter respiratori secondari infect sign secondari infect mask overlap typic sign classic swine fever mislead farmer veterinarian death occur month recoveri product antibodi occur adult breed anim displai sever clinic sign antibodi classic swine fever viru detect week post infect onward patholog visibl post mortem examin frequent observ lymph node kidnei lymph node swollen oedemat haemorrhag haemorrhag kidnei vari size visibl petechia ecchymot haemorrhag similar haemorrhag observ urinari bladder larynx epiglotti heart widespread serosa abdomen chest purul enceph present lesion due secondari infect mislead veterinarian infarct spleen consid pathognomon infrequ gener acut form african swine fever lead clinic patholog pictur similar classic swine fever present haemorrhag skin ear easi detect lead suspicion acut african classic swine fever diseas similar lesion acut classic swine fever consid case suspect erysipela porcin reproduct respiratori syndrom coumarin poison purpura haemorragica post wean multisystem wast syndrom porcin dermat nephropathi syndrom salmonella pasteurella infect enter respiratori syndrom fever respond antibiot treatment classic swine fever viru shed saliva urin faec onset clinic sign death classic swine fever viru shed semen chronic form chronic infect occur pig develop effect immun respons classic swine fever viru initi sign chronic infect similar acut infect predomin specif sign present intermitt fever chronic enter wast typic haemorrhag skin miss pig show clinic sign diseas month death classic swine fever viru constantli shed onset clinic sign death antibodi temporarili detect serum sampl patholog typic haemorrhag organ serosa observ anim show chronic diarrhoea necrot lesion common ileum ileocaec valv rectum clinic sign chronic classic swine fever specif diseas consid differenti diagnosi increas bodi temperatur necessarili present anim infect hold fever detect pig prenat form late onset diseas classic swine fever viru pass placenta pregnant anim infect foetus sow diseas clinic outcom tran placent infect foetus depend larg stage gestat viral virul infect earli pregnanc result abort stillbirth mummif malform lead reduct fertil index hold infect sow dai pregnanc lead birth persist viraem piglet clinic normal birth surviv month birth show poor growth wast occasion congenit tremor infect refer quot late onset classic swine fever quot piglet plai crucial role spread diseas mainten viru persist popul constantli shed viru death detect classic swine fever difficult breed pig hold infect mild confus patholog condit reduc fertil abort caus classic swine fever viru parvoviru infect prr leptospirosi aujeszki diseas materi abort due classic swine fever infect distinguish patholog abort due diseas agent case suspicion infecti diseas reproduct tract investig classic swine fever immedi carri hold question consid risk due locat hold area classic swine fever occur feral pig case common infecti diseas reproduct tract exclud chapter iii guidelin main criteria consid recognit hold classic swine fever suspect hold decis recognis hold suspect hold basi find criteria ground clinic patholog find pig main clinic patholog find consid fever increas morbid mortal fever haemorrhag syndrom fever neurolog symptom fever unknown origin treatment antibiot fail improv health state abort increas fertil problem month congenit tremor piglet chronic diseas anim growth retard runt young anim petechi ecchymot haemorrhag lymph node kidnei spleen bladder larynx infarct haematoma notabl spleen button ulcer larg intestin chronic case ileo caecal junction epidemiolog find main epidemiolog find consid pig direct indirect contact pig hold proven infect classic swine fever hold suppli pig subsequ shown infect classic swine fever sow artifici insemin semen origin suspect sourc indirect direct contact feral pig popul classic swine fever occur pig outdoor region feral pig infect classic swine fever pig fed swill suspicion swill treat inactiv classic swine fever viru exposur occur due person enter hold transport find relat result serolog test main laboratori find consid serolog reaction caus unnot classic swine fever viru infect vaccin cross reaction antibodi classic swine fever pestivirus detect singleton reactor chapter iv check sampl procedur guidelin procedur clinic examin sampl pig suspect hold member state ensur clinic examin sampl laboratori investig carri suspect hold confirm exclud classic swine fever accord guidelin procedur laid subparagraph irrespect adopt measur refer articl direct ec hold question guidelin procedur appli case diseas classic swine fever consid differenti diagnosi includ occas clinic sign epidemiolog pattern diseas observ pig suggest low probabl occurr classic swine fever case pig suspect infect classic swine fever viru measur refer articl direct ec adopt suspect hold question case suspicion classic swine fever pig slaughterhous mean transport guidelin procedur laid subparagraph appli mutati mutandi offici veterinarian visit suspect hold confirm rule classic swine fever check product health record hold carri record inspect unit hold carri select pig clinic examin clinic examin includ take bodi temperatur primarili concern pig group pig sick anorex pig pig recent recov diseas pig recent introduc confirm outbreak suspect sourc pig unit recent visit extern visitor recent close contact classic swine fever suspect infect pig riski contact potenti sourc classic swine fever viru identifi pig sampl serolog test classic swine fever case result test rule classic swine fever contact pig inspect suspect hold presenc pig group pig refer subparagraph compet author prejudic measur appli hold question accord direct ec take account epidemiolog situat carri examin hold question accord subparagraph ensur blood sampl laboratori test pig hold question case sampl procedur laid subparagraph guidanc purpos adopt maintain measur laid articl direct ec pend investig hold question rule suspicion classic swine fever refer made paragraph clinic examin hold question carri pig select random unit risk introduct classic swine fever viru identifi suspect minimum number pig examin detect fever occur preval confid unit case breed sow minimum number sow examin detect fever occur preval confid semen collect centr boar examin dead moribund pig detect suspect hold post mortem examin carri prefer pig pig death shown show evid sign diseas high fever recent dead examin shown lesion suggest classic swine fever due epidemiolog situat investig deem clinic examin laid subparagraph blood sampl laid subparagraph carri unit dead moribund pig post mortem examin carri contact pig irrespect presenc absenc lesion suggest classic swine fever sampl organ tissu pig subject post mortem examin collect virolog test accord chapter sampl prefer collect recent dead pig post mortem examin carri compet author ensur precaut hygien measur prevent diseas spread case moribund pig kill human accord council direct eec clinic sign lesion suggest classic swine fever detect suspect hold compet author deem find suffici confirm outbreak classic swine fever laboratori test blood sampl laboratori test suspect pig pig unit suspect pig accord procedur laid minimum number sampl serolog test detect seropreval confid unit question case breed sow minimum number sow sampl detect seropreval confid semen collect centr blood sampl boar number sampl virolog test accord instruct compet author account rang test perform sensit laboratori test epidemiolog situat suspicion classic swine fever hold question relat result previou serolog test addit blood sampl pig refer subparagraph indent procedur appli seroposit pig pregnant sow prefer euthanas subject post mortem examin prior kill blood sampl serolog test foetus subject examin classic swine fever viru viru antigen viru genom accord chapter vi detect intrauterin infect seroposit pig sow suckl piglet blood sampl piglet subject examin classic swine fever viru viru antigen viru genom refer chapter vi blood sampl sow serolog test examin carri suspect hold clinic sign lesion suggest classic swine fever detect laboratori test deem compet author rule classic swine fever sampl procedur laid subparagraph guidanc purpos sampl procedur hold pig kill confirm diseas order manner introduct classic swine fever viru infect hold length time elaps introduct establish pig kill hold confirm outbreak accord articl direct ec blood sampl serolog test random pig kill minimum number pig sampl detect seropreval confid pig unit hold sampl virolog test accord instruct compet author account rang test perform sensit laboratori test epidemiolog situat case secondari outbreak compet author decid derog subparagraph establish ad hoc sampl procedur take account epidemiolog inform sourc mean viru introduct hold potenti spread diseas hold sampl procedur pig kill prevent measur suspect hold order classic swine fever confirm rule addit epidemiolog inform gain pig kill prevent measur suspect hold accord provis articl articl direct ec blood sampl serolog test blood tonsil sampl virolog test accord procedur laid subparagraph sampl primarili concern pig show sign post mortem lesion suggest classic swine fever contact pig pig riski contact infect suspect pig suspect contamin classic swine fever viru pig sampl accord instruct compet author account epidemiolog situat case sampl procedur laid fourth subparagraph guidanc purpos pig proceed unit hold sampl random case minimum number sampl serolog test detect seropreval confid unit question case breed sow minimum number sow sampl detect seropreval confid semen collect centr blood sampl boar type sampl virolog test test accord instruct compet author account rang test perform sensit test epidemiolog situat check sampl procedur authoris move pig hold locat protect surveil zone case pig slaughter kill prejudic provis articl subparagraph direct ec order authoris move pig hold locat protect surveil zone accord articl direct clinic examin carri offici veterinarian carri hour period move pig accord provis laid case pig move hold addit investig carri accord subparagraph clinic examin pig carri unit hold pig move case pig older month examin includ take temperatur proport pig minimum number pig check detect fever occur preval confid unit case breed sow minimum number sow examin detect fever occur preval confid unit sow move boar boar move examin case pig move slaughterhous process plant place kill slaughter addit investig carri accord subparagraph clinic examin pig carri unit pig move case pig older month examin includ take temperatur proport pig minimum number pig check detect fever occur preval confid unit question case breed sow boar minimum number pig examin detect fever occur preval confid subunit pig move pig refer subparagraph slaughter kill blood sampl serolog test blood tonsil sampl virolog test pig proceed unit pig move minimum number sampl detect seropreval viru preval confid unit case breed sow boar minimum number pig sampl detect seropreval viru preval confid subunit pig type sampl test accord instruct compet author account rang test perform sensit test epidemiolog situat clinic sign post mortem lesion suggest classic swine fever detect pig slaughter kill derog subparagraph provis sampl laid appli check sampl procedur hold relat popul pig introduc hold accord articl articl subparagraph direct ec sampl procedur appli case sentinel pig reintroduc blood sampl serolog test random number pig detect seropreval confid unit hold case total popul blood sampl serolog test random number pig detect seropreval confid unit hold case breed sow boar number sampl detect seropreval confid introduct pig compet author ensur case diseas death pig hold due unknown reason pig question immedi test classic swine fever provis appli restrict refer articl subparagraph articl subparagraph sentenc direct ec lift hold question sampl procedur hold protect zone lift restrict order measur refer articl direct ec lift protect zone hold zone clinic examin carri accord procedur laid blood sampl serolog test laid subparagraph minimum number blood sampl detect seropreval confid pig unit hold case breed sow minimum number sampl detect seropreval confid semen collect centr blood sampl boar sampl procedur hold surveil zone lift restrict order restrict refer articl direct ec lift surveil zone clinic examin carri hold zone accord procedur laid addit blood sampl serolog test pig hold pig month ag compet author deem classic swine fever spread unnot breed sow hold sampl deem compet author semen collect centr blood sampl serolog test carri hold locat surveil zone number blood sampl hold accord compet author deem classic swine fever spread unnot breed sow sampl carri unit anim serolog monitor sampl procedur area classic swine fever suspect occur confirm feral pig case serolog monitor feral pig area classic swine fever confirm suspect occur size geograph area target popul sampl previous defin order establish number sampl sampl size establish function estim number live anim function shot anim data popul densiti size geograph area sampl identifi take account continu presenc feral pig presenc natur artifici barrier effici prevent larg continu movement anim circumst occur case larg area recommend identifi sampl area km popul feral pig live prejudic provis articl direct ec minimum number pig sampl defin sampl area detect seropreval confid purpos anim sampl area identifi recommend area hunt pressur higher regularli perform select hunt carri diseas control measur approxim sampl anim belong month year ag class year ag class year ag class area hunt pressur low absent anim sampl ag class sampl perform short time prefer month ag sampl anim identifi teeth erupt collect sampl virolog test feral pig shot found dead carri laid chapter virolog monitor shot feral pig deem primarili carri anim month year sampl laboratori accompani questionnair refer articl direct ec chapter gener procedur criteria collect transport sampl gener procedur criteria sampl carri suspect hold map hold prepar epidemiolog unit hold identifi time deem sampl pig pig sampl uniqu mark easili sampl prejudic chapter iv sampl serolog test piglet week sampl laboratori accompani form accord requir establish compet author form includ detail histori pig sampl clinic sign post mortem lesion observ case pig hold clear inform ag categori hold origin pig sampl provid recommend locat pig sampl hold record uniqu identif mark collect sampl virolog test detect classic swine fever viru antigen genom dead euthanis pig tonsil spleen kidnei tissu suitabl sampl addit recommend collect sampl lymphat tissu retro pharyng parotid mandibular mesenter lymph node sampl ileum case autolys carcass entir long bone sternum specimen choic anticoagul blood clot blood sampl collect pig show sign fever sign diseas accord instruct compet author virolog test recommend case sick anim limit monitor purpos anim show clinic sign object larg scale sampl detect classic swine fever viru pig incub period tonsil sampl transport sampl recommend sampl transport store leak proof contain frozen cool refriger temperatur deliv laboratori quickli packag ic pack wet ic insid cool tissu organ separ seal plastic bag properli label larger strong outer contain pack suffici absorb materi protect damag absorb leakag directli transport laboratori compet personnel order rapid reliabl transport ensur packag label address recipi laboratori messag promin displai anim patholog materi perish fragil open classic swine fever laboratori laboratori receiv sampl inform advanc time mode arriv sampl air transport sampl commun refer laboratori classic swine fever member state germani countri packag label iata regul chapter vi principl virolog test evalu result detect viru antigen fluoresc antibodi test fat principl test detect viral antigen thin cryosect organ materi pig suspect infect classic swine fever viru intracellular antigen detect fitc conjug antibodi posit result confirm repeat stain specif monoclon antibodi suitabl organ tonsil kidnei spleen lymph node ileum smear bone marrow cell case feral pig organ autolys test perform dai organ sampl obtain dead anim screen purpos limit confid test result limit doubt stain consider experi perform test acquir organ test autolys elisa antigen detect viral antigen detect elisa techniqu sensit antigen elisa high score posit result anim show clinic sign classic swine fever elisa antigen detect recommend sampl anim clinic sign patholog lesion diseas suitabl investig individu anim suitabl sampl leukocyt serum coagul blood suspens organ refer subparagraph pig suspect infect classic swine fever viru elisa carri dai perform automat equip import advantag larg number sampl process short period time recommend elisa antigen give satisfactori result refer materi present commerci elisa sensit viru isol cell cultur sensit significantli blood sampl piglet adult pig viru isol viru isol base incub sampl materi suscept cell cultur porcin origin classic swine fever viru occur sampl replic cell amount detect immunostain infect cell conjug antibodi classic swine fever specif antibodi requir differenti diagnosi respect pestivirus prefer sampl isol classic swine fever viru leukocyt plasma blood obtain coagul blood sampl organ refer viru isol suit investig sampl small number anim mass surveil viru isol procedur labour intens requir dai result cell cultur passag order small amount viru sampl detect lead investig time dai final result obtain autolys sampl cytotox cell cultur limit recommend perform viru isol case previou confirm classic swine fever method refer test confirm posit result prior antigen elisa pcr fat indirect peroxidas stain method classic swine fever viru isol obtain viru characteris includ genet type molecular epidemiolog classic swine fever viru isol primari outbreak primari case feral pig case slaughterhous mean transport genet type nation refer laboratori member state laboratori authoris member state question commun refer laboratori accord case viru isol commun refer laboratori viru collect delai detect viru genom polymeras chain reaction pcr appli detect viru genom blood tissu organ sampl small fragment viral rna transcrib dna fragment amplifi pcr detect quantiti test detect genom sequenc viru pcr posit infecti viru present autolys tissu sampl convalesc pig pcr small number sampl carefulli select suspect anim materi abort fetus carcass wild boar method choic materi autolys viru isol due cytotox suitabl sampl materi diagnost pcr organ viru isol unclot blood pcr perform hour requir laboratori equip separ facil skill staff advantag infecti viru particl replic laboratori method highli sensit contamin easili occur lead fals posit result stringent qualiti control procedur essenti method pestiviru classic swine fever specif requir confirmatori test sequenc pcr product evalu result virolog test virolog test essenti confirm classic swine fever viru isol consid refer virolog test confirmatori test recommend case posit fat elisa pcr result detect clinic sign lesion diseas doubt case primari outbreak classic swine fever confirm clinic sign lesion diseas detect pig question antigen genom detect test posit result secondari outbreak classic swine fever confirm addit epidemiolog link confirm outbreak case clinic sign lesion diseas detect pig question antigen genom detect test posit result primari case classic swine fever feral pig confirm viru isol antigen genom detect test posit result case classic swine fever feral pig epidemiologi link previous confirm case found confirm antigen genom detect test posit result posit result classic swine fever genom antigen detect test requir test question perform classic swine fever viru specif antibodi primer test specif classic swine fever viru pestiviru specif repeat classic swine fever specif reagent genet type classic swine fever viru isol genet type classic swine fever viru isol achiev determin nucleotid sequenc portion viru genom specif part noncod region glycoprotein gene similar sequenc obtain previou viru isol outbreak diseas caus recognis strain support refut hypothes transmiss rout provid epidemiolog trace genet type classic swine fever viru isol major import determin sourc diseas close relationship virus obtain outbreak absolut proof direct epidemiolog link viru type perform nation laboratori laboratori authoris diagnos classic swine fever short delai origin sampl viru isol commun refer laboratori type data type sequenc classic swine fever viru isol laboratori authoris diagnos classic swine fever forward commun refer laboratori order inform enter databas laboratori inform includ databas nation refer laboratori member state purpos public scientif journal request laboratori question commun refer laboratori guarante confidenti data publish chapter vii principl serolog test evalu result basic principl diagnost classic swine fever viru infect pig antibodi detect serum sampl week infect pig recov diseas protect neutralis antibodi detect year lifetim antibodi sporad detect termin stage lethal diseas anim pig chronic form classic swine fever antibodi detect dai end month post infect pig infect utero immunotoler homologu classic swine fever viru produc specif antibodi antibodi matern origin detect dai life half life matern antibodi viraem healthi piglet week found piglet older month classic swine fever antibodi matern origin detect antibodi classic swine fever viru serum plasma sampl carri assist diagnosi classic swine fever suspect hold establish ag infect case confirm outbreak monitor surveil purpos serolog test limit detect classic swine fever case recent infect hold seroposit pig low neutralis titr indic recent infect week pig high neutralis titr viru enter hold month locat seroposit pig hold provid valuabl inform classic swine fever viru enter hold accur evalu result serolog test carri take account clinic virolog epidemiolog find framework enquiri carri case suspicion confirm classic swine fever accord articl direct ec recommend serolog test viru neutralis test vnt elisa test choic serolog diagnosi classic swine fever qualiti effici serolog diagnosi perform nation laboratori regularli check framework inter laboratori comparison test period organis commun refer laboratori vnt base determin viru neutralis activ antibodi serum sampl express neutralis end point constant amount classic swine fever viru incub dilut serum screen purpos sera initi dilut full titrat fold dilut serum start prepar dilut mix equal volum viru suspens infecti dose tcid incub mixtur inocul cell cultur incub dai incub period cultur fix viral replic infect cell detect immun label system neutralis peroxidas link antibodi npla neutralis immunofluoresc nif assai result vnt express reciproc initi serum dilut half inocul cell cultur end point fail show viral replic specif label point dilut level estim final dilut system base actual dilut serum neutralis reaction addit viru ad cell suspens vnt sensit reliabl test detect antibodi classic swine fever viru recommend serolog examin singl anim herd basi cross neutralis antibodi specif rumin pestivirus infect pig detect test vnt detect antibodi bvd viru bd viru follow princip mention conduct differenti diagnosi classic swine fever pestivirus strain neutralis test accord recommend commun refer laboratori elisa techniqu specif monoclon antibodi develop base format competit block elisa competit elisa competit block elisa base monoclon antibodi serum sampl antibodi classic viru bind select peroxidas conjug monoclon antibodi viru antigen inhibit result reduc signal competit elisa bind serum antibodi antigen measur directli peroxidas conjug anti pig antibodi qualiti control sensit specif batch elisa regularli perform nation laboratori make panel refer sera provid commun refer laboratori panel includ sera pig earli phase classic swine fever viru infect dai post infect sera convalesc pig dai post infect sera pig infect rumin pestivirus elisa serolog diagnosi classic swine fever recognis refer sera convalesc pig result obtain refer sera repeat recommend detect posit sera earli phase show minimum cross reaction sera pig infect rumin pestivirus result obtain refer sera pig earli phase infect give indic sensit elisa sensit elisa regard lower vnt recommend screen test herd basi elisa requir specialis facil perform rapidli autom system vnt elisa ensur identif classic swine fever infect convalesc stage free interfer cross react antibodi rumin pestivirus interpret serolog result differenti diagnosi infect due rumin pestivirus bvdv bdv prejudic provis articl articl direct ec case detect classic swine fever viru neutralis titr equal higher serum sampl collect pig posit elisa result serum sampl group pig measur refer articl direct ec immedi appli continu appli hold question sampl collect hold test vnt compar end point titrat neutralis antibodi classic swine fever viru rumin pestivirus compar test show antibodi rumin pestivirus evid lower fold antibodi titr classic swine fever viru suspicion classic swine fever rule reason exist warrant continu applic measur refer articl direct ec hold question compar test show viru neutralis titr pig equal higher titr equal higher titr pestivirus compet author ensur classic swine fever confirm provid epidemiolog evid diseas found hold question prejudic provis articl direct ec epidemiolog evid diseas found result previou test inconclus compet author ensur hold question measur refer articl direct ec continu appli investig carri quickli confirm rule classic swine fever accord chapter iv check test refer subparagraph classic swine fever rule blood sampl serolog test carri hold week elaps previou check framework sampl pig sampl test sampl compar serolog test previous collect sampl detect sero convers classic swine fever viru rumin pestivirus check test classic swine fever confirm measur refer articl direct ec lift chapter viii discriminatori test case emerg vaccin suitabl discriminatori test distinguish pig vaccin pig natur infect classic swine fever viru chapter ix minimum safeti requir classic swine fever laboratori minimum requir laid tabl fulfil laboratori classic swine fever viru manipul small amount requir viru isol neutralis test post mortem examin process tissu fat serolog inactiv antigen carri lower contain level provid basic hygien post oper disinfect safe dispos tissu sera appli addit requir laid tabl fulfil laboratori procedur involv extens multipl viru carri requir laid tabl fulfil laboratori anim experi classic swine fever viru carri case stock classic swine fever viru secur storag deep frozen freez dri recommend freezer refriger virus classic swine fever materi unrel classic swine fever diagnosi individu ampoul label comprehens record maintain viru stock date result qualiti control check record virus ad stock detail sourc virus issu laboratori recommend bio safe unit classic swine fever viru work support area classic swine fever viru manipul area prepar glasswar media mainten prepar infect cell cultur process sera serolog test method live classic swine fever viru provis administr cleric support tabl principl biolog contain diagnost laboratori gt tabl gt tabl bio safeti requir experiment anim room gt tabl gt decid number sampl laboratori test sensit test consid number anim sampl higher manual sensit test high singleton reactor titr viru neutralis antibodi rang borderlin case strongli posit sampl singleton reactor show decreas constant titr gener pig herd give rise fals posit reaction pig vaccin classic swine fever convent vaccin found seroposit due vaccin due silent infect vaccin anim circumst pig herd antibodi rumin pestivirus caus bovin viral diarrhoea border diseas pig direct contact cattl sheep infect bvd viru bd viru pig contact materi contamin rumin pestivirus current serolog test classic swine fever small proport sera give fals posit result due lack specif test system due sera singleton reactor case classic swine fever suspect hold limit number young pig proport infect sow small case higher number sow sampl derog provid articl direct ec appli sampl concern unit hold pig kill prejudic examin sampl carri remain pig hold carri accord instruct compet author compet author limit applic prevent kill part hold pig suspect infect contamin classic swine fever viru accord articl direct ec sampl concern unit hold measur appli prejudic examin sampl carri remain pig hold carri accord instruct compet author case classic swine fever suspect hold limit number young pig proport infect sow small case higher number sow sampl commun refer laboratori unlimit permit receiv diagnost sampl classic swine fever viru isol copi import permit request laboratori transport attach envelop packag classic swine fever elisa antigen commerci valid type sampl