oj commiss direct novemb lai commun method analysi test partli wholli dehydr preserv milk human consumpt eec commiss european commun regard treati establish european econom commun regard council direct eec decemb approxim law member state relat partli wholli dehydr preserv milk human consumpt articl thereof articl direct eec composit partli wholli dehydr preserv milk requir verifi commun method analysi desir adopt initi seri method respect studi complet measur provid direct accord opinion stand committe foodstuff adopt direct articl member state measur ensur analys verif criteria set annex carri accord method annex ii articl altern method singl determin sampl analys method test report refer annex ii state method emploi articl member state bring forc law regul administr provis compli direct month notif forthwith inform commiss thereof articl direct address member state brussel novemb commiss etienn davignon member commiss annex scope commun method analysi partli wholli dehydr preserv milk direct gener provis ii determin dry matter unsweeten condens high fat milk method annex ii unsweeten condens milk method annex ii unsweeten condens partli skim milk method annex ii unsweeten condens skim milk method annex ii sweeten condens milk method annex ii sweeten condens partli skim milk method annex ii sweeten condens skim milk method annex ii iii determin moistur dri high fat milk high fat milk powder method annex ii dri milk milk powder method annex ii dri partli skim milk partli skim milk powder method annex ii dri skim milk skim milk powder method annex ii iv determin fat unsweeten condens high fat milk method annex ii unsweeten condens milk method annex ii unsweeten condens partli skim milk method annex ii unsweeten condens skim milk method annex ii sweeten condens milk method annex ii sweeten condens partli skim milk method annex ii sweeten condens skim milk method annex ii dri high fat milk high fat milk powder method annex ii dri milk milk powder method annex ii dri partli skim milk partli skim milk powder method annex ii dri skim milk skim milk powder method annex ii determin sucros sweeten condens milk method annex ii sweeten condens partli skim milk method annex ii sweeten condens skim milk method annex ii vi determin lactic acid lactat dri high fat milk high fat milk powder method annex ii dri milk milk powder method annex ii dri partli skim milk partli skim milk powder method annex ii dri skim milk skim milk powder method annex ii vii determin phosphatas activ dri high fat milk high fat milk powder method annex ii dri milk milk powder method annex ii dri partli skim milk partli skim milk powder method annex ii dri skim milk skim milk powder method annex ii annex ii method analysi relat composit partli wholli dehydr preserv milk product intend human consumpt gener provis prepar sampl chemic analysi unsweeten condens high fat milk unsweeten condens milk unsweeten condens partli skim milk unsweeten condens skim milk shake invert close open slowli pour milk contain close hermet mix repeat transfer ensur remain fat milk adher wall end mix sampl close contain content homogen warm contain waterbath shake vigor minut hour remov contain waterbath cool room temperatur remov lid mix content contain spoon spatula fat separ sampl test store cool place sweeten condens milk sweeten condens partli skim milk sweeten condens skim milk can warm close waterbath minut open mix content spatula spoon make upward downward circular movement order obtain intim mixtur top bottom layer content ensur remain milk adher wall end incorpor sampl pour content contain provid air tight lid close contain store cool place tube cut end pour content contain provid air tight lid cut tube lengthwis scrape materi adher interior mix carefulli rest content store contain cool place dri high fat milk high fat milk powder dri milk milk powder dri partli skim milk partli skim milk powder dri skim milk skim milk powder transfer milk powder clean dry contain air tight lid capac volum powder close contain immedi mix milk powder repeatedli shake invert contain prepar sampl exposur milk powder atmospher avoid minim absorpt moistur reagent water mention made water dissolut dilut wash purpos distil water deminer water equival puriti refer made quot dissolut quot quot solut quot quot dilut quot indic quot dissolut water quot quot solut water quot quot dilut water quot meant chemic chemic recogn analyt reagent qualiti equip list equip list equip item special item specif analyt balanc analyt balanc mean balanc capabl weigh mg express result calcul percentag result calcul percentag mass sampl receiv laboratori number signific figur result signific figur justifi precis method analysi test report test report identifi method analysi result obtain addit mention detail procedur method analysi option circumst influenc result obtain test report give inform complet identif sampl method determin dry matter content oven scope field applic method determin dry matter content unsweeten condens high fat milk unsweeten condens milk unsweeten condens partli skim milk unsweeten condens skim milk sweeten condens milk sweeten condens partli skim milk sweeten condens skim milk definit dry matter content condens milk dry matter content determin method principl amount sampl dilut water mix sand dri temperatur mass dry mass dry matter calcul percentag mass sampl reagent quartz sand sea sand treat hydrochlor acid size grain mm pass micron siev retain micron siev meet control test heat sand hour dry oven add ml water heat oven hour cool reweigh differ mass exce mg treat sand hydrochlor acid solut dai mix occasion wash water acid reaction disappear wash water chlorid free dry test apparatu analyt balanc metal dish prefer nickel aluminium stainless steel dish lid fit readili remov suitabl dimens diamet mm depth mm atmospher pressur dry oven ventil thermostat control temperatur regul temperatur uniform oven desicc freshli activ silica gel water content indic equival desicc glass rod flatten end length fit insid metal dish waterbath boil procedur place sand short glass rod dish cover dish content lid place dish content lid oven heat hour replac lid transfer dish desicc cool room temperatur accur weigh nearest mg tilt sand side dish introduc clear space sampl sweeten condens milk unsweeten condens milk replac lid accur weigh nearest mg remov lid add ml water aid glass rod mix liquid sand liquid portion leav rod mixtur place dish boil waterbath water evapor take minut stir mixtur time time rod mass aerat mass dry form cake lai rod insid dish place dish lid oven half hour replac lid transfer dish desicc cool room temperatur accur weigh nearest mg replac dish lid oven uncov dish heat lid hour repeat process repeat process differ mass success weigh mg mass increas increas mass occur lowest mass obtain calcul final weight record express result method calcul content dry matter calcul percentag mass sampl repeat differ result determin carri simultan rapid success sampl analyst condit exce dry matter product calcul total milk solid milk solid fat total milk solid content sweeten condens milk total dry matter obtain method annex ii sucros obtain method annex ii milk solid fat content sweeten condens milk total dry matter obtain method annex ii sucros content obtain method annex ii fat content obtain method annex ii milk solid fat content unsweeten condens milk total dry matter obtain method annex ii fat content obtain method annex ii method determin moistur oven scope field applic method determin loss mass dry dri high fat milk high fat milk powder dri milk milk powder dri partli skim milk partli skim milk powder dri skim milk skim milk powder definit moistur content loss mass dry determin method principl residu mass test portion determin dry atmospher pressur oven constant mass loss mass calcul percentag mass sampl apparatu analyt balanc dish prefer nickel aluminium stainless steel glass dish lid fit readili remov suitabl dimens diamet mm depth mm atmospher pressur dry oven ventil thermostat control temperatur regul temperatur uniform oven desicc freshli activ silica gel water content indic equival desicc procedur uncov dish place lid oven heat hour place lid dish transfer cover dish desicc cool room temperatur accur weigh nearest mg introduc approxim dri milk sampl dish cover dish lid accur weigh nearest mg cover dish quickli uncov dish put lid oven hour replac lid transfer cover dish desicc cool room temperatur accur weigh nearest mg quickli uncov dish heat lid hour oven repeat process repeat process decreas mass success weigh exce mg mass increas increas mass occur lowest mass obtain calcul final weight record express result method calcul calcul loss mass dry sampl express percentag mass formula repeat differ result determin carri simultan rapid success sampl analyst condit exce moistur product method determin fat content condens milk rose gottlieb method scope field applic method determin fat content unsweeten condens high fat milk unsweeten condens milk unsweeten condens partli skim milk unsweeten condens skim milk sweeten condens milk sweeten condens partli skim milk sweeten condens skim milk definit fat content condens milk fat content determin method principl fat content determin extract fat ammoniac alcohol solut sampl diethyl ether light petroleum evapor solvent weigh residu calcul percentag mass sampl principl se gottlieb reagent reagent conform requir blank test reagent redistil presenc butterfat ml solvent ammonia solut approxim nh densiti approxim ml stronger solut concentr ethanol ethanol denatur methanol ethyl methyl keton light petroleum diethyl ether peroxid free note test peroxid add ml ether small glass stopper cylind previous rins ether ml freshli prepar potassium iodid solut shake stand minut yellow colour observ layer note diethyl ether maintain free peroxid ad wet zinc foil complet immers dilut acidifi copper sulphat solut minut subsequ wash water litr approxim mm zinc foil cut strip long reach halfwai contain light petroleum petroleum ether boil rang mix solvent prepar shortli mix equal volum diethyl ether light petroleum mix solvent replac diethyl ether light petroleum apparatu analyt balanc suitabl extract tube flask provid ground glass stopper closur unaffect solvent flask thin wall flat bottom ml capac atmospher pressur dry oven ventil thermostat control adjust oper anti bump granul fat free porou friabl glass bead piec silicon carbid materi option claus siphon fit extract tube centrifug option procedur blank test time determin fat content sampl carri blank determin ml water type extract apparatu reagent amount procedur exclud claus blank exce mg reagent check impur reagent reagent purifi replac determin dry flask requir anti bump granul promot gentl boil subsequ remov solvent oven half hour flask cool temperatur balanc room accur weigh cool flask nearest mg stir prepar sampl immedi weigh nearest mg sampl sweeten sampl unsweeten directli differ extract apparatu add water ml shake gentli slight warm product complet dispers sampl dispers complet determin repeat add ml ammonia volum stronger solut mix add ml ethanol mix liquid gentli unclos apparatu add ml diethyl ether cool run water close apparatu shake vigor invert repeatedli minut remov stopper carefulli add ml light petroleum millilitr rins stopper insid neck apparatu allow rins run apparatu close replac stopper shake invert repeatedli second shake vigor centrifug apparatu stand upper liquid layer clear distinctli separ lower aqueou layer altern carri separ suitabl centrifug note centrifug driven phase motor spark occur care avoid explos fire ether vapour come broken tube remov stopper rins insid neck apparatu millilitr mix solvent rins run apparatu carefulli transfer supernat layer decant mean siphon prepar flask note transfer made siphon add water order rais interfac layer aid decant rins insid neck apparatu tip lower part siphon millilitr mix solvent rins apparatu run flask rins insid neck siphon run extract apparatu make extract repeat procedur inclus ml diethyl ether ml light petroleum make extract repeat procedur omit final rins note mandatori carri extract analys skim unsweeten condens milk skim sweeten condens milk sampl carefulli evapor distil solvent includ ethanol flask small capac remov solvent extract appreci odour solvent place flask side oven heat hour remov flask oven cool temperatur balanc room accur weigh nearest mg repeat heat period minut differ mass success weigh mg mass increas increas mass occur lowest mass obtain calcul final weight record add ml light petroleum order confirm extract matter wholli solubl warm gentli swirl solvent fat dissolv extract matter wholli solubl light petroleum mass fat differ weight determin stage insolubl matter present case doubt complet extract fat flask repeat wash warm light petroleum allow undissolv materi settl decant rins neck flask time heat flask side hour oven cool temperatur balanc room weigh nearest mg mass fat differ mass obtain final mass express result calcul repeat differ result determin carri obtain simultan rapid success sampl analyst condit exce fat product method determin fat content dri milk rose gottlieb method scope field applic method determin fat content dri high fat milk high fat milk powder dri milk milk powder dri partli skim milk partli skim milk powder dri skim milk skim milk powder definit fat content dri milk fat content determin method principl fat content determin extract fat ammoniac alcohol solut sampl diethyl ether light petroleum evapor solvent weigh residu calcul percentag mass sampl principl se gottlieb reagent reagent conform requir blank test reagent redistil presenc butterfat ml solvent ammonia solut approxim nh densiti approxim ml stronger solut concentr ethanol ethanol denatur methanol ethyl methyl keton light petroleum diethyl ether peroxid free note test peroxid add ml ether small glass stopper cylind previous rins ether ml freshli prepar potassium iodid solut shake stand minut yellow colour observ layer note diethyl ether maintain free peroxid ad wet zinc foil complet immers dilut acidifi copper sulphat solut minut subsequ wash water litr approxim mm zinc foil cut strip long reach halfwai contain light petroleum petroleum ether boil rang mix solvent prepar shortli mix equal volum diethyl ether light petroleum mix solvent replac diethyl ether light petroleum apparatu analyt balanc suitabl extract tube flask provid ground glass stopper closur unaffect solvent flask thin wall flat bottom ml capac atmospher pressur dry oven ventil thermostat control adjust oper anti bump granul fat free porou friabl glass bead piec silicon carbid materi option claus waterbath siphon fit extract tube centrifug option procedur blank test time determin fat content sampl carri blank determin ml water type extract apparatu reagent amount procedur exclud claus blank exce mg reagent check impur reagent reagent purifi replac determin dry flask requir anti bump granul promot gentl boil subsequ remov solvent oven half hour flask cool temperatur balanc room accur weigh cool flask nearest mg accur weigh nearest mg directli differ extract apparatu milk powder partli skim skim milk powder add ml water shake gentli milk powder complet dispers heat sampl add ml ammonia volum stronger solut heat waterbath minut shake occasion cool run water add ml ethanol mix liquid gentli unclos apparatu add ml diethyl ether cool run water close apparatu shake vigor invert repeatedli minut remov stopper carefulli add ml light petroleum millilitr rins stopper insid neck apparatu allow rins run apparatu close replac stopper shake invert repeatedli second shake vigor centrifug apparatu stand upper liquid layer clear distinctli separ lower aqueou layer altern carri separ suitabl centrifug note centrifug driven phase motor spark occur care avoid explos fire ether vapour come broken tube remov stopper rins insid neck apparatu millilitr mix solvent rins run apparatu carefulli transfer supernat layer decant mean siphon prepar flask note transfer made siphon add water order rais interfac layer aid decant rins insid neck apparatu tip lower part siphon millilitr mix solvent rins apparatu run flask rins insid neck siphon run extract apparatu make extract repeat procedur inclus ml diethyl ether ml light petroleum make extract repeat procedur omit final rins note mandatori carri extract analys dri skim milk sampl carefulli evapor distil solvent includ ethanol flask small capac remov solvent extract appreci odour solvent place flask side oven heat hour remov flask oven cool temperatur balanc room previous accur weigh nearest mg repeat heat period minut differ mass success weight mg mass increas increas mass occur lowest mass obtain calcul final weight record add ml light petroleum order confirm extract matter wholli solubl warm gentli swirl solvent fat dissolv extract matter wholli solubl light petroleum mass fat differ weight determin stage insolubl matter present case doubt complet extract fat flask repeat wash warm light petroleum allow undissolv materi settl decant rins neck flask time heat flask side hour oven cool temperatur balanc room weigh nearest mg mass fat differ mass final mass express result repeat differ result determin carri simultan rapid success sampl analyst condit exce fat product except skim milk powder differ exce fat product method determin sucros content polarimeter method scope field applic method determin sucros content sweeten condens milk sweeten condens partli skim milk sweeten condens skim milk sampl invert sugar definit sucros content sweeten condens milk sucros content determin method principl method base principl clerget invers mild treatment sampl acid produc complet hydrolysi sucros lactos sugar sucros content obtain chang rotat power solut clear filtrat sampl mutarot lactos prepar treatment solut ammonia neutral clear success addit zinc acet potassium hexacyanoferr ii solut portion filtrat sucros hydrolyz manner rotat filtrat invers sucros content calcul formula reagent zinc acet solut dissolv crystal zinc acet dihydr zn ml glacial acet acid water make ml water potassium hexacyanoferr ii solut dissolv crystal potassium hexacyanoferr ii trihydr fe cn water make ml water hydrochlor acid solut ammonia solut acet acid solut bromothymol blue indic solut ethanol apparatu balanc sensit mg polarimet tube dm calibr length polarimet saccarimet polarimet sodium light mercuri green light mercuri vapour lamp prism special wratten screen read accuraci angular degre saccarimet intern sugar scale white light pass filter mm solut potassium bichrom sodium light read accuraci intern sugar scale water bath regul procedur control determin order standard procedur reagent apparatu carri control determin duplic mixtur milk pure sucros mixtur skim milk pure sucros condens milk sucros calcul sugar content formula substitut formula quantiti milk fat protein content milk formula valu found differ determin weigh mg approxim mix sampl ml glass beaker add ml hot water mix transfer mixtur quantit ml measur flask rins beaker success quantiti water total volum ml mix cool room temperatur add ml dilut ammonia solut mix stand minut neutral ammonia ad equival quantiti dilut solut acet acid determin exact number ml titrat ammonia solut bromothymol blue indic mix add gentli mix rotat tilt flask ml zinc acet solut add ml potassium hexacyanoferr ii solut acet solut bring content flask make ml mark water note stage addit water reagent made manner avoid format air bubbl object view mix carri rotat flask shake air bubbl found present make ml volum remov assist temporarili connect flask vacuum pump rotat flask close flask dry stopper mix vigor shake stand minut filter dry filter paper reject ml filtrat direct polar determin optic rotat filtrat invers pipett ml filtrat obtain ml volumetr flask add ml hydrochlor acid ml hydrochlor acid place flask waterbath minut ensur entir bulb flask immers mix rotatori movement minut time content flask attain temperatur bath cool make volum water mix stand hour temperatur invert polar determin rotat invert solut temperatur liquid polar tube differ measur temperatur correct refer appli express result method calcul valu invers factor formula give accur valu sourc light correct concentr temperatur sodium light polarimet angular degre mercuri green light polarimet angular degre white light dichrom filter saccharimet intern sugar scale degre formula percentag total sugar invert solut polar temperatur invert solut polarimetr read note percentag total sugar invert solut calcul direct read chang invers usual manner usual valu specif rotat sucros lactos invert sugar correct accur approxim normal condens milk correct neglect close note variat temperatur make differ direct read variat invert read necessit correct correct accur repeat differ result determin carri simultan rapid success sampl analyst condit exce sucros condens milk method determin lactic acid lactat content scope field applic method determin lactic acid lactat express lactic acid content dri high fat milk high fat milk powder dri milk milk powder dri partli skim milk partli skim milk powder dri skim milk skim milk powder definit lactic acid lactat content dri milk lactic acid lactat express lactic acid content determin method principl fat protein lactos simultan remov solut sampl addit copper sulphat calcium hydroxid filtrat lactic acid lactat filtrat convert acetaldehyd concentr sulphur acid presenc copper ii sulphat lactic acid content determin colorimetr hydroxydiphenyl lactic acid lactat content express mg lactic acid solid fat reagent copper ii sulphat solut dissolv copper ii sulphat cuso water dilut ml water calcium hydroxid suspens grind calcium hydroxid ca mortar water total ml suspens freshli prepar calcium hydroxid suspens grind calcium hydroxid ca mortar water total ml suspens freshli prepar sulphur acid copper ii sulphat solut add ml sulphur acid ml copper ii sulphat solut hydroxydiphenyl solut dissolv shake heat slightli hydroxydiphenyl ml aqueou solut sodium hydroxid naoh ml dilut ml water volumetr flask solut brown colour glass bottl dark cool place colour tubid occur maximum shelf life hour lactic acid standard solut dissolv shortli lithium lactat ch chohcooli water dilut ml volumetr flask ml solut correspond mg lactic acid standard reconstitut milk analys advanc sampl high qualiti dri milk prepar calibr curv select sampl lowest lactic acid content mg lactic acid solid fat follow oper procedur apparatu analyt balanc spectrophotomet suitabl read wavelength nm waterbath mortar pestl filter paper schleicher schull whatman equival test tube pyrex equival dimens mm note glasswar perfectli clean design sole determin rins glasswar precipit residu concentr hydrochlor acid wash procedur blank test carri blank test place ml water ml graduat tube treat tube inclus blank measur water exce equival mg lactic acid solid fat reagent check impur reagent reagent replac carri blank test time analysi sampl determin note avoid contamin impur saliva sweat determin solid fat content sampl subtract fat content obtain method moistur content obtain method weigh sampl nearest add quantiti sampl ml water mix pipett ml solut obtain ml graduat tube dilut water ml add slowli shake ml copper ii sulphat solut stand minut add slowli shake ml calcium hydroxid suspens ml calcium hydroxid suspens dilut ml water shake vigor stand minut filter discard run pipett ml filtrat test tube add tube mean burett graduat pipett ml sulphur acid copper ii sulphat solut mix heat boil water bath minut cool ambient temperatur run water add drop hydroxydiphenyl reagent shake vigor spread reagent evenli liquid place tube waterbath leav minut shake time time place tube boil waterbath second cool ambient temperatur run water measur optic densiti blank test hour wavelength optic densiti exce highest point standard curv repeat test adequ dilut filtrat obtain prepar standard pipett ml reconstitut milk ml graduat tube pipett tube ml standard solut obtain rang standard mg ad lactic acid solid fat dri milk dilut water ml treat measur optic densiti standard blank test wavelength plot diagram optic densiti quantiti lactic acid mg mg mg mg mg solid fat draw fit straight line point prepar standard curv move line parallel pass origin express result method calcul convert optic densiti measur mg lactic acid solid fat sampl refer standard curv multipli result dilut factor filtrat dilut repeat differ result determin carri simultan rapid success sampl analyst condit exce mg lactic acid solid fat content mg higher valu differ exce lowest method determin phosphatas activ modifi sander sager procedur scope field applic method describ determin phosphatas activ dri high fat milk high fat milk powder dri milk milk powder dri partli skim milk partli skim milk powder dri skim milk skim milk powder definit phosphatas activ dri milk measur quantiti activ alkalin phosphatas present express quantiti phenol ug liber ml reconstitut milk determin procedur principl phosphatas activ dri milk determin abil phosphatas liber phenol disodiumphenylphosph quantiti phenol liber prescrib condit determin spectrophotometr measur colour develop gibb reagent reagent solut barium borat hydroxid buffer ph dissolv barium hydroxid ba water dilut ml dissolv boric acid bo water dilut ml warm solut mix shake cool mixtur room temperatur adjust ph barium hydroxid solut filter store solut tightli stopper contain dilut buffer equal quantiti water solut colour develop buffer dissolv sodium metabor nabo nabo sodium chlorid nac water dilut ml water solut buffer substrat solut dissolv disodiumphenylphosph na po ml solut add drop solut stand minut extract colour ml butanol repeat colour extract separ discard butanol solut dai refriger develop extract colour pipett ml solut ml volumetr flask make volum solut prepar buffer solut immedi solut precipit dissolv zinc sulphat znso copper ii sulphat cuso water make ml water solut gibb reagent dissolv dibromoquinon chloroimid br nc ml ethanol store solut dark glass bottl refriger discard reagent discolour colour dilut buffer dilut ml solut colour develop buffer ml water copper sulphat solut dissolv copper ii sulphat cuso water make ml water phenol standard solut dissolv pure phenol water make ml volumetr flask water solut store month refriger dilut ml solut ml water dilut solut ug phenol ml prepar dilut solut boil distil water butanol apparatu analyt balanc waterbath thermostat control spectrophotomet suitabl read wavelength nm filter paper schleicher schull whatman equival filter paper waterbath boil aluminium foil procedur precaut avoid direct exposur sunlight glasswar stopper remov materi perfectli clean recommend rins boil water treat steam avoid plastic materi stopper phenol saliva phosphatas contamin trace saliva carefulli avoid prepar sampl weigh sampl dissolv ml water temperatur dissolv powder circumst exce determin introduc test tube ml reconstitut milk prepar heat tube boil water minut cover tube waterbath beaker aluminium foil ensur entir tube heat cool cold water room temperatur tube blank test subsequ oper treat tube ident add ml solut mix place tube waterbath incub minut waterbath shake period transfer tube immedi boil waterbath heat minut cool room temperatur cold water add ml solut mix filter dry filter paper discard filtrat clear liquid obtain put ml filtrat test tube add ml solut ml solut mix colour develop room temperatur minut direct sunlight measur optic densiti sampl solut blank wavelength repeat determin optic densiti solut standard sampl ug phenol prepar limit exceed dilut suitabl volum reconstitut milk suitabl volum milk carefulli boil inactiv phosphatas present prepar standard curv pipett ml volumetr flask ml standard solut dilut make volum water dilut ug phenol ml pipett ml water ml standard solut test tube order obtain seri sampl blank obtain ml water ug phenol pipett success test tube ml solut copper ii sulphat ml colour dilut buffer solut ml water ml solut mix leav test tube minut room temperatur direct sunlight measur absorb solut tube compar blank wavelength prepar standard curv plot absorb valu quantiti phenol ug express result calcul convert figur determin ug phenol refer standard curv calcul phosphatas activ express ug phenol ml reconstitut milk formula phosphatas activ quantiti phenol ug dilut multipli result obtain dilut factor repeat differ result determin carri simultan rapid success sampl analyst condit exce ug phenol liber ml reconstitut milk method determin phosphatas activ aschaffenburg mullen procedur scope field applic method describ determin phosphatas activ dri high fat milk high fat milk powder dri milk milk powder dri partli skim milk partli skim milk powder dri skim milk skim milk powder definit phosphatas activ dri milk measur quantiti activ alkalin phosphatas present product express quantiti nitrophenol microgram liber ml reconstitut sampl condit principl reconstitut sampl dilut buffer substrat ph incub temperatur hour alkalin phosphatas present sampl circumst liber nitrophenol ad disodium nitrophenyl phosphat nitrophenol liber determin direct comparison standard colour glass simpl compar reflect light reagent sodium carbon bicarbon buffer solut dissolv anhydr sodium carbon sodium bicarbon water dilut ml volumetr flask water buffer substat dissolv disodium nitrophenylphosph sodium carbon bicarbon buffer dilut ml volumetr flask buffer solut stabl store refriger month colour control test carri store solut precaut number clarif solut zinc sulphat solut dissolv zinc sulphat znso water dilut ml volumetr flask water potassium hexacyanoferr ii solut dissolv potassium hexacyanoferr ii trihydr fe cn dilut ml volumetr flask water apparatu analyt balanc waterbath thermostat control compar special disc standard colour glass calibr ug nitrophenol ml milk mm cell procedur precaut test tube empti rins water wash hot water alkalin deterg rins clean hot tap water final rins water dri pipett rins clean cold tap water immedi rins water dri test tube stopper rins hot tap water immedi boil minut water buffer substrat solut remain stabl month store refriger instabl denot format yellow colour whilst test read boil product control buffer substrat solut recommend solut colour read excess ug read mm cell compar distil water mm cell separ pipett sampl avoid contamin pipett saliva test expos direct sunlight time prepar sampl dissolv powder ml water temperatur dissolv powder exce determin pipett ml buffer substrat clean dry test tube ml reconstitut sampl test stopper tube mix invers place water bath time place water bath control tube ml buffer substrat ml boil reconstitut sampl similar test hour remov tube water bath add ml zinc sulphat precipit replac stopper shake vigor stand minut add ml potassium hexacyanoferr ii precipit mix filter flute filter paper collect clear filtrat clean test tube transfer filtrat mm cell compar filtrat boil sampl control compar special disc express result calcul direct read obtain record ug nitrophenol ml sampl ml reconstitut sampl repeat differ result determin carri simultan rapid success sampl analyst condit exce ug nitrophenol liber ml reconstitut milk