commiss decis march fix total volatil basic nitrogen tvb limit valu categori fisheri product analysi method ec commiss european commun regard treati establish european commun regard council direct eec juli lai health condit product place market fisheri product chapter ii annex thereto check provid direct eec prevent fisheri product unfit human consumpt market compris chemic check includ check total volatil basic nitrogen tvb set level tvb exceed case speci categori analysi method analysi method scientif recogn check tvb continu matter routin advis refer method case doubt result event disput measur provid decis accord opinion stand veterinari committe adopt decis articl unprocess fisheri product belong speci categori list annex regard unfit human consumpt organolept assess rais doubt fresh chemic check reveal tvb limit exceed milligram nitrogen gram flesh speci refer point annex milligram nitrogen gram flesh speci refer point annex milligram nitrogen gram flesh speci refer point annex articl refer method check tvb limit method involv distil extract deprotein perchlor acid set annex ii distil refer paragraph perform apparatu compli principl diagram annex iii routin method check tvb limit microdiffus method conwai byrn direct distil method antonacopoulo distil extract deprotein trichloracet acid codex alimentariu committe fish fisheri product sampl consist hundr gram flesh point mix grind articl member state recommend offici laboratori matter routin refer method refer articl case doubt event disput result analysi perform routin method refer method check result articl decis address member state brussel march commiss franz fischler member commiss annex speci categori tvb limit fix sebast spp helicolenu dactylopteru sebastichthi capensi speci belong pleuronectida famili except halibut hippoglossu spp salmo salar speci belong merlucciida famili speci belong gadida famili annex ii determin concentr volatil nitrogen base tvb fish fish product refer procedur purpos area applic method describ refer procedur identifi nitrogen concentr volatil nitrogen base total volatil base tvb fish fish product procedur applic tvb concentr mg mg definit tvb concentr understood nitrogen content volatil nitrogen base determin procedur concentr state term mg descript volatil nitrogen base extract sampl solut perchlor acid alkalin extract submit steam distil volatil base compon absorb acid receiv tvb concentr determin titrat absorb base chemic reagent grade chemic water distil deminer puriti solut understood aqueou solut perchlor acid solut ml sodium hydroxid solut ml hydrochlor acid standard solut mol note automat distil apparatu titrat place hydrochlor acid standard solut mol boric acid solut ml silicon anti foam agent phenolphtalein solut ml ethanol indic solut tashiro mix indic methyl red methylen blue dissolv ml ethanol instrument accessori meat grinder produc suffici homogen fish minc high speed blender revolut min min flute filter diamet mm quick filter burett ml graduat ml apparatu steam distil apparatu regul amount steam produc constant amount steam period time ensur addit alkal substanc result free base escap execut warn work perchlor acid strongli corros caution prevent measur sampl prepar paragraph arriv prepar sampl sampl analys ground carefulli meat grinder secton ground sampl weigh suitabl contain mix ml perchlor acid solut state section homogen minut blender section filter extract obtain dai temperatur approxim steam distil ml extract obtain section put apparatu steam distil secton check suffici alkalin extract drop phenolphtalein section ad ad drop silicon anti foam agent ml sodium hydroxid solut section ad extract steam distil begin immedi steam distil regul ml distil produc minut distil outflow tube submerg receiv ml boric acid solut section drop indic solut ad minut distil end distil outflow tube remov receiv wash water volatil base contain receiv solut determin titrat standard hydrochlor solut secton ph end point titrat duplic analys requir appli method correct differ duplic higher mg blank blind test carri section extract ml perchlor acid solut section calcul tvb titrat receiv solut hydrochlor acid tvb concentr calcul equat tvb express mg sampl pv volum hydrochlor acid solut ml sampl volum hydrochlor acid solut ml blanc weight sampl remark duplic analys requir appli method correct differ duplic higher mg check equip distil solut nh cl equival mg tvb standard deviat reproduc sr mg standard deviat compar sr mg annex iii gt start graphic gt steam gener distil tube steam inject tube sampl extract cooler cool water end condens flask beaker boric acid tvb steam distil apparatu gt end graphic gt