commiss regul ec march amend annex council direct eec amend decis ec commiss european commun regard treati establish european commun regard council direct eec june anim health problem affect intra commun trade bovin anim swine amend commiss decis ec articl paragraph thereof octob scientif committe anim health anim welfar adopt report modif technic annex direct eec account scientif develop tuberculosi brucellosi enzoot bovin leucosi accord report test brucellosi carri line manual standard diagnost test vaccin edit offic intern de epizooti oie august oie publish fourth edit manual includ modif descript test brucellosi amend annex direct eec lai test procedur applic surveil trade purpos commun reflect oie standard account advic scientif committe nation refer laboratori member state cooper framework european union network nation refer laboratori brucellosi commiss decis ec april approv test detect antibodi bovin brucellosi framework council direct eec amend measur provid regul accord opinion stand veterinari committe adopt regul articl annex direct eec replac annex present regul articl decis ec amend articl replac quot articl complement fixat test buffer brucella antigen test elisa test carri accord provis annex direct eec approv certif purpos quot annex delet articl regul enter forc dai public offici journal european commun regul bind entireti directli applic member state brussel march commiss david byrn member commiss oj oj sanco oj annex quot annex brucellosi identif agent demonstr modifi acid fast immunospecif stain organ brucella morpholog abort materi vagin discharg milk presumpt evid brucellosi support serolog test isol speci biovar identifi phage lysi oxid metabol test cultur biochem serolog criteria techniqu media standardis interpret result conform oie manual standard diagnost test vaccin fourth edit chapter bovin brucellosi chapter caprin ovin brucellosi chapter porcin brucellosi immunolog test standard brucella abortu biovar weybridg strain usda strain prepar antigen rose bengal test rbt serum agglutin test sat complement fixat test cft milk ring test mrt standard refer serum rbt sat cft mrt oie intern refer standard serum oieiss name intern anti brucella abortu serum isab standard refer sera elisa oieiss weak posit oie elisa standard serum oieelisawpss strong posit oie elisa standard serum oieelisaspss neg oie elisa standard serum oieelisanss list standard sera veterinari laboratori agenc vla weybridg unit kingdom oieiss oieelisawpss oieelisaspss oieelisanss intern primari standard secondari refer nation standard quot work standard quot establish test member state enzym link immunosorb assai elisa bind assai detect bovin brucellosi serum milk materi reagent techniqu interpret result valid accord principl laid chapter oie manual standard diagnost test vaccin fourth edit includ laboratori diagnost studi standardis test standardis test procedur individu serum sampl pre dilut oieiss pre dilut oieelisawpss pre dilut oieelisaspss made neg serum neg pool sera give posit reaction pre dilut oieiss pre dilut oieelisawpss pre dilut oieelisaspss made neg serum neg pool sera give neg reaction oieelisanss give neg reaction standardis test procedur pool serum sampl pre dilut oieiss pre dilut oieelisawpss pre dilut oieelisaspss made neg serum neg pool sera dilut neg sera number sampl make pool give posit reaction oieelisanss give neg reaction test adequ detect evid infect singl anim group anim sampl serum pool standardis test procedur pool milk whei sampl pre dilut oieiss pre dilut oieelisawpss pre dilut oieelisaspss made neg serum neg pool sera dilut neg milk give posit reaction oieelisanss dilut neg milk give neg reaction test adequ detect evid infect singl anim group anim sampl milk whei pool condit elisa diagnosi bovin brucellosi abovement calibr condit elisa serum sampl diagnost sensit elisa equal greater rbt cft take account epidemiolog situat emploi abovement calibr condit elisa pool milk sampl diagnost sensit elisa equal greater mrt take account epidemiolog situat averag expect extrem husbandri system elisa certif purpos accord articl establish mainten herd statu accord annex ii pool sampl serum carri test result undoubtedli relat individu anim includ pool confirmatori test carri sampl serum individu anim elisa sampl milk milk collect farm dairi cow milk method measur ensur sampl examin undoubtedli relat individu anim milk deriv confirmatori test carri sampl serum individu anim complement fixat test cft antigen repres bacteri suspens phenol salin nacl phenol veron buffer antigen deliv concentr state provid dilut factor bottl label antigen store frozen serum inactiv bovin serum minut porcin serum minut order carri genuin reaction test procedur complement dose higher minimum total haemolysi carri complement fixat test control made time control anti complementari effect serum control antigen control sensitis red blood cell control complement control posit serum sensit start reaction control specif reaction neg serum calcul result oieiss intern cft unit icftu ml oieiss test method result titr toieiss test result test serum titr ttestserum express icftu ml order convert express titr icftu factor convert titr unknown test serum ttestserum test method icftu express found formula gt pic file quot en tif quot gt content intern cft unit ml test serum icftutestserum formula gt pic file quot en tif quot gt interpret result serum icftu ml consid posit milk ring test mrt antigen repres bacteri suspens phenol salin nacl phenol stain haematoxylin antigen store frozen antigen sensit standardis relat oieiss antigen produc posit reaction dilut oieiss neg milk dilut neg ring test made sampl repres content milk churn content bulk tank farm milk sampl frozen heat subject violent shake reaction carri method column milk mm height volum milk ml ml ml standardis stain antigen ad column milk mm height volum milk ml ml standardis stain antigen ad volum milk ml ml standardis stain antigen ad mixtur milk antigen incub minut posit neg work standard subsequ hour incub increas sensit test interpret result neg reaction colour milk colourless cream posit reaction ident colour milk cream colourless milk colour cream rose bengal plate test rbt antigen repres bacteri suspens buffer brucella antigen diluent ph stain rose bengal dye antigen deliv readi store frozen antigen prepar refer cell concentr sensit standardis relat oieiss antigen produc posit reaction serum dilut neg reaction dilut rbt carri manner serum μl mix equal volum antigen white tile enamel plate produc zone approxim cm diamet mixtur rock gentli minut ambient temperatur observ good light agglutin autom method sensit accur manual method interpret result visibl reaction consid posit excess dry round edg posit neg work standard includ seri test serum agglutin test sat antigen repres bacteri suspens phenol salin nacl phenol formaldehyd antigen deliv concentr state provid dilut factor bottl label edta ad antigen suspens mm final test dilut reduc level fals posit serum agglutin test subsequ ph readjust antigen suspens oieiss intern unit agglutin antigen prepar refer cell concentr sensit standardis relat oieiss antigen produc agglutin final serum dilut agglutin final serum dilut advis compar reactiv previous standardis batch antigen panel defin sera test perform tube micropl mixtur antigen serum dilut incub hour dilut prepar serum dilut suspect serum made read reaction posit limit made median tube micropl method interpret result degre brucella agglutin serum express iu ml serum iu ml consid posit complementori test brucellosi skin test bst condit bst brucellosi skin test purpos certif intra commun trade brucellosi skin test specif test detect brucellosi unvaccin anim diagnosi made basi posit intraderm reaction bovin anim test neg result serolog test defin annex react posit bst regard infect bovin anim test posit result serolog test defin annex subject bst order support interpret serolog test result brucellosi free offici free herd cross reaction antibodi bacteria exclud test carri standardis defin brucellosi allergen prepar smooth lipopolysaccharid lp antigen provok specif inflammatori reaction interfer subsequ serolog test prepar brucellin inra prepar smooth strain melitensi requir product detail section chapter oie manual standard diagnost test vaccin fourth edit test procedur volum ml brucellosi allergen inject intraderm caudal fold skin flank side neck test read hour skin thick inject site measur vernier callip inject examin interpret result strong reaction easili recognis local swell indur skin thicken mm consid posit reaction bst competit enzym link immunosorb assai celisa condit celisa celisa purpos certif intra commun trade celisa shown higher specif indirect elisa order support interpret serolog test result test procedur test carri accord prescript oie manual standard diagnost test vaccin fourth edit chapter nation refer laboratori task respons nation refer laboratori respons approv result valid studi demonstr reliabl test method member state determin maximum number sampl pool elisa kit calibr standard secondari refer nation standard sera quot work standard quot primari intern standard serum refer paragraph qualiti check antigen elisa kit batch member state cooper european union network nation refer laboratori brucellosi list nation refer laboratori belgium centr étude de recherch vétérinair agrochimiqu cerva coda groeselenberg bruxel brussel denmark danish veterinari institut bulowsvej dk copenhagen germani bundesinstitut für gesundheitlichen verbraucherschutz und veterinärmedizin bgvv national veterinärmedizinisch referenzlabor für brucellos postfach berlin greec veterinari laboratori larissa depart microbiolog km nation road larissa trikala gr larissa spain laboratorio central de veterinaria de santa fe camino del jau santa fe granada franc laboratoir nation oie fao de référenc pour la brucellos agenc français de sécurité sanitair de aliment afssa bp maison alfort cedex ireland brucellosi laboratori model farm road cork ireland itali istituto zooprofilattico sperimental dell abruzzo del molis campo boario teramo luxembourg state laboratori veterinarian medicin avenu gaston diderich luxembourg netherland centraal instituut voor dierziektecontrol cidc lelystad houtribweg box aa lelystad nederland austria bundesanstalt für veterinärmedizinisch untersuchungen robert koch gass modl portug laboratório nacion de investigação veterinária lniv estrada de benfica lisboa finland nation veterinari food research institut hämeenti po box fin helsinki sweden nation veterinari institut uppsala unit kingdom fao collabor centr refer research brucellosi veterinari laboratori agenc haw addleston surrei kt nb unit kingdom immunodiagnost depart veterinari scienc divis stonei road stormont belfast bt sd unit kingdom purpos annex dilut make liquid reagent express dilut quot