commiss direct april amend direct eec approxim law member state relat method analysi check composit cosmet product eec commiss european commun regard treati establish european econom commun commiss direct eec approxim law member state relat method analysi check composit cosmet product lai common method analysi identif determin free formaladehyd light scientif technic data prove amend method analysi measur provid direct conform opinion committe adapt technic progress direct remov technic barrier trade cosmet product sector adopt decis articl chapter iv annex direct eec replac text set annex hereto articl member state bring forc law regul administr provis compli direct decemb forthwith inform commiss thereof provis adopt paragraph refer expressli direct articl direct address member state brussel april commiss karel van miert member commiss oj annex iv identif determin free formaldehyd purpos scope method describ identif determin presenc formaldehyd donor applic cosmet product identif gener determin pentan dion colorimetri method appli formaldehyd preserv formaldehyd donor case result exce maximum permit concentr method confirm determin presenc formaldehyd donor method mention deriv formaldehyd donor split lead result high combin polymer formaldehyd separ free formaldehyd liquid chromatographi definit free formaldehyd content sampl determin method express percentag mass identif principl free combin formaldehyd sulphur acid medium turn schiff reagent pink mauv reagent reagent analyt puriti water deminer fuchsin sodium sulphit hydrat concentr hydrochlor acid sulphur acid schiff reagent mg fuchsin weigh beaker dissolv ml water cool add sodium sulphit make ml week procedur weigh sampl ml beaker add drop sulphur acid ml schiff reagent reagent absolut colourless shake leav stand minut pink mauv tint observ minut formaldehyd present excess determin free combin method procedur gener determin pentan dion colorimetri principl formaldehyd react pentan dion presenc ammonium acet form diacetyl dihydrolutidin extract butan ol absorb extract measur nm reagent reagent analyt puriti water deminer anhydr ammonium acet concentr acet acid pentan dion freshli distil reduc pressur mm hg exhibit absorpt nm butan ol hydrochlor acid hydrochlor acid approxim sodium hydroxid starch solut freshli prepar european pharmacopoeia ml water edit part vii formaldehyd standard iodin solut standard sodium thiosulph solut pentan dion reagent ml volumetr flask dissolv ammonium acet ml pentan dion ml acet acid make ml water ph solut reagent freshli prepar reagent pentan dion formaldehyd standard stock solut pour formaldehyd ml volumetr flask make ml water determin strength solut remov ml add ml standard iodin solut ml sodium hydroxid solut stand minut acidifi ml hcl determin excess iodin standard sodium thiosulph solut starch solut indic ml iodin consum equival mg formaldehyd formaldehyd standard dilut solut dilut formaldehyd stock solut success water ml solut mg formaldehyd calcul exact content apparatu standard laboratori apparatu phase separ filter whatman ps equival centrifug water bath set spectrophotomet glass cell optic path cm procedur sampl solut ml volumetr flask weigh quantiti test sampl presum quantiti formaldehyd mg make ml water mix solut check ph close dilut hydrochlor acid solut ml erlenmey flask add ml solut ml pentan dion reagent deminer water final volum ml refer solut interfer due background colour test sampl elimin refer solut ml erlenmey flask add ml solut ml reagent deminer water final volum ml blank test ml erlenmey flask add ml pentan dion reagent deminer water final volum ml determin shake mixtur immers erlenmey flask water bath minut cool minut bath ic water transfer ml separ funnel ml butan ol rins flask ml water shake mixtur vigor second separ filter butan ol phase measur cell phase separ filter centrifug gn minut measur absorb nm extract sampl solut extract refer solut similarli measur absorb extract blank solut butan ol oper carri minut moment erlenmey flask water bath calibr curv ml erlenmey flask place ml dilut standard solut ml pentan dion reagent deminer water final volum ml continu measur absorb butan ol repeat procedur ml dilut standard solut obtain color reagent proce construct calibr curv subtract absorb obtain beer law valid mg formaldehyd calcul subtract read calibr curv amount mg formaldehyd sampl solut calcul formaldehyd content sampl aid formula formaldehyd content sier mass tie test portion repeat formaldehyd content differ result determin parallel carri sampl exce determin pentan dion colorimetri determin free formaldehyd lead result greater maximum concentr provid direct eec label product greater product label procedur appli determin presenc formaldehyd donor principl separ formaldehyd transform yellow lutidin deriv reaction pentan dion post column reactor deriv obtain detect absorb nm reagent reagent analyt puriti water deminer hplc grade water water equival qualiti anhydr ammonium acet concentr acet acid pentan dion anhydr disodium phosphat orthophosphor acid hplc grade methanol dichloromethan formaldehyd sodium hydroxid hydrochlor acid hydrochlor acid starch solut freshli prepar european pharmacopoeia standard iodin solut standard sodium thiosulph solut mobil phase aqueou solut disodium phosphat adjust ph orthophosphor acid post column reagent ml volumetr flask dissolv ammonium acet ml acet acid ml pentan dion make ml water reagent light conserv time maximum dai chang colour observ formaldehyd standard stock solut pour formaldehyd ml volumetr flask make ml water determin strength solut remov ml add ml standard iodin solut ml sodium hydroxid solut stand minut acidifi ml hcl titrat excess standard iodin solut standard sodium thiosulph solut starch solut indic ml iodin solut iso formaldehyd standard dilut solut dilut stock solut initi strength mobil phase ml solut contrain mg formaldehyd calcul exact content apparatu standard laboratori apparatu hplc pump pulsat free low pressur pulsat free pump reagent hplc pump inject valv ml loop post column reactor compon litr neck flask litr flask heater vigreux column minimum plate air cool stainless steel tube heat exchang mm intern diamet mm length mm teflon tube mm intern diamet mm length french knit appendix piec dead volum valco equival union dead volum post column modul appli biosystem pcr equival fit ml reactor membran filter pore size mm sep pakr cartridg equival readi colulmn bischoff hypersil rp type nc refer mm length mm intern diamet mm dupont zorbax od mm length mm intern diamet mm phase sep spherisorb od mm length mm intern diamet mm pre column bischoff hypersil rp refer mm length mm equival column pre column connect mean ecotub system refer bischoff equival assembl apparatu shown block diagram appendix connect inject short case stainless steel tube reactor outlet detector inlet intend cool mixtur prior detect temperatur detector unknown constant uv visibl detector record centrifug ultrason bath vibrat stirrer vortex equival procedur calibr curv produc plot peak height function concentr formaldehyd standard dilut prepar standard solut dilut formaldehyd refer solut mobil phase ml solut dilut ml mg ml ml solut dilut ml mg ml ml solut dilut ml mg ml ml solut dilut ml mg ml standard solut hour laboratori temperatur freshli prepar linear calibr curv good concentr mg ml prepar sampl emuls cream make base eyelin stopper ml flask weigh nearest quantiti test sampl presum quantiti mg formaldehyd add ml dichloromethan ml hydrochlor acid accur measur mix vibrat stirrer mean ultrason bath separ phase centrifug gn minut wash cartridg ml methanol condit ml water pass ml aqueou phase extract condit cartridg discard ml recov fraction lotion shampoo weigh stopper ml flask nearest quantiti test sampl presum quantiti mg formaldehyd make ml mobil phase filter solut filter inject pass cartridg condit solut inject immedi prepar chromatograph condit flowrat mobil phase ml min reagent flowrat ml min total flowrat detector outlet ml min inject volum ml elut temperatur case difficult separ immers column bath melt ic wait temperatur stabil min temperatur post column reaction detect nm nb entir chromatograph system post column flush water system dai flush flush methanol recondit system pass water avoid recrystal calcul emuls formaldehyd content lotion siampoo tii psase tie formthla vepsom ps sier mass tie sampl analys ps formaldeivd psonpsentr mg ml read calibr curv repeat content formaldehyd differ result determin parallel carri sampl exce content formaldehyd differ result determin parallel carri sampl exce equival mg formaldehyd iso appendix intruct quot french knit quot accessori requir wooden bobbin extern diamet cm hole cm diamet made centr insert steel nail shown figur distanc nail cm cm hole rigid needl crotchet hook type loop teflon tube mm teflon tube intern diamet mm procedur start quot french knit quot teflon tube thread top bobbin bottom central hole leav cm tube protrud bottom bobbin enabl chain pull knit process wind tube nail turn shown figur top bottom french knit protect metal ring compress screw care crush teflon pull tight wind tube nail turn make stitch lift lower tube upper tube hook figur repeat process nail order anti clockwis direct desir length knit produc leav cm tube close chain thread tube loop pull gentli close end chain nb french knit manufactur post column reactor market supelco schemat diagram bobbin figur figur figur tube figur tube form quot stitch quot lift lower tube unbroken line tube dot line figur appendix hplc pump inject valv column pre column reagent pump piec dead volum piec vortex union dead volum french knit reactor neck flask boil water flask heater coolant stainless steel heat exchang tube heat exchang visibl uv detector pcr post column modul eluent reagent reagent light conserv time maximum dai chang colour observ formaldehyd standard stock solut pour formaldehyd ml volumetr flask make ml water determin strength solut remov ml add ml standard iodin solut ml sodium hydroxid solut stand minut acidifi ml hcl titrat excess standard iodin solut standard sodium thiosulph solut starch solut indic ml iodin solut equival mg iso formaldehyd standard dilut solut dilut stock solut initi strength mobil phase ml solut contrain mg formaldehyd calcul exact content apparatu standard laboratori apparatu hplc pump pulsat free low pressur pulsat free pump reagent hplc pump inject valv loop post column reactor compon litr neck flask litr flask heater vigreux column minimum plate air cool stainless steel tube heat exchang mm intern diamet mm length mm teflon tube mm intern diamet mm length french knit appendix piec dead volum valco equival union dead volum post column modul appli biosystem pcr equival fit ml reactor membran filter pore size sep pakr cartridg equival readi colulmn bischoff hypersil rp type nc refer length mm intern diamet mm dupont zorbax od length mm intern diamet mm phase sep spherisorb od length mm intern diamet mm pre column bischoff hypersil rp refer length mm equival column pre column connect mean ecotub system refer bischoff equival assembl apparatu shown block diagram appendix connect inject short case stainless steel tube reactor outlet detector inlet intend cool mixtur prior detect temperatur detector unknown constant uv visibl detector record centrifug ultrason bath vibrat stirrer vortex equival procedur calibr curv produc plot peak height function concentr formaldehyd standard dilut prepar standard solut dilut formaldehyd refer solut mobil phase ml solut dilut ml ml ml solut dilut ml ml ml solut dilut ml ml ml solut dilut ml ml standard solut hour laboratori temperatur freshli prepar linear calibr curv good concentr ml prepar sampl emuls cream make base eyelin stopper ml flask weigh nearest quantiti test sampl presum quantiti formaldehyd add ml dichloromethan ml hydrochlor acid accur measur mix vibrat stirrer mean ultrason bath separ phase centrifug gn minut wash cartridg ml methanol condit ml water pass ml aqueou phase extract condit cartridg discard ml recov fraction lotion shampoo weigh stopper ml flask nearest quantiti test sampl presum quantiti formaldehyd make ml mobil phase filter solut filter inject pass cartridg condit solut inject immedi prepar chromatograph condit flowrat mobil phase ml min reagent flowrat ml min total flowrat detector outlet ml min inject volum elut temperatur case difficult separ immers column bath melt ic wait temperatur stabil min temperatur post column reaction detect nm nb entir chromatograph system post column flush water system dai flush flush methanol recondit system pass water avoid recrystal calcul emuls formaldehyd content ml read calibr curv repeat content formaldehyd differ result determin parallel carri sampl exce content formaldehyd differ result determin parallel carri sampl exce formaldehyd iso appendix intruct quot french knit quot accessori requir wooden bobbin extern diamet cm hole cm diamet made centr insert steel nail shown figur distanc nail cm cm hole rigid needl crotchet hook type loop teflon tube mm teflon tube intern diamet mm procedur start quot french knit quot teflon tube thread top bobbin bottom central hole leav cm tube protrud bottom bobbin enabl chain pull knit process wind tube nail turn shown figur top bottom french knit protect metal ring compress screw care crush teflon pull tight wind tube nail turn make quot stitch lift lower tube upper tube hook figur repeat process nail order anti clockwis direct desir length knit produc leav cm tube close chain thread tube loop pull gentli close end chain nb french knit manufactur post column reactor market supelco schemat diagram bobbin figur figur figur tube figur tube form quot stitch quot lift lower tube unbroken line tube dot line figur appendix hplc pump inject valv column pre column reagent pump piec dead volum piec vortex union dead volum quot french knit reactor neck flask boil water flask heater coolant stainless steel heat exchang tube heat exchang visibl uv detector pcr post column modul eluent reagent