commiss regul ec decemb lai detail rule appli regul ec grant aid skim milk skim milk powder intend anim feed sale skim milk powder commiss european commun regard treati establish european commun regard council regul ec common organis market milk milk product articl thereof regul ec replac council regul eec amend regul ec council regul eec juli lai gener rule grant aid skim milk skim milk powder feed amend commiss regul ec account arrang past experi commiss regul eec juli rule grant aid skim milk process compound feedingstuff skim milk powder intend feed calv amend regul ec amend simplifi time amend regul interest clariti recast incorpor commiss regul eec novemb sale invit tender skim milk powder manufactur compound feedingstuff amend regul eec amend regul ec eec june fix amount aid skim milk skim milk powder feed amend regul eec goal aid measur provid articl regul ec enabl return obtain milk protein link payment aid milk protein content skim milk skim milk powder ensur skim milk skim milk powder aid grant fact feed end aid grant skim milk skim milk powder process compound feedingstuff denatur accord requir prevent aid paid product commiss regul ec derog monitor provis regul eec check provid regul derog account regul ec repeal aid grant compound feedingstuff meet minimum standard composit customarili observ industri reach final stage industri manufactur inspect purpos product packag enabl identifi member state allow requir satisfi special pack compound feedingstuff lucern meal ad requir case transport tanker contain practis user method transport subject special inspect arrang aid paid inspect final cut price skim milk skim milk powder monitor undertak benefit aid offer adequ guarante process undertak approv compet agenc member state concern account system adapt requir aid arrang refer method applic analys provid aid scheme refer made list publish year commiss regul ec novemb establish rule applic refer routin method analysi qualiti evalu milk milk product common market organis refer method laid determin quantiti skim milk powder compound feedingstuff presenc rennet whei powder skim milk powder qualiti starch skim milk powder method laid regul stand invit tender organis sale skim milk powder public stock order ensur equal access potenti purchas arriv sell price reflect market condit verifi actual end skim milk powder intend manufactur compound feedingstuff price offer differ consider ag powder offer sale locat fix differenti minimum price regul set time limit entri storag purpos sale commiss regul eec decemb set latest time entri storag skim milk powder sold regul eec amend regul ec repeal arrang laid commiss regul eec juli detail rule grant aid skim milk feed amend regul eec prove difficult implement check beneficiari problemat quantiti skim milk benefit measur fallen sharpli recent year scheme margin impact balanc market milk product addit market skim milk continu support aid grant skim milk incorpor compound feedingstuff aid measur provid regul eec abolish regul repeal manag committe milk milk product deliv opinion time limit set chairman adopt regul chapter gener provis articl regul lai detail rule appli regul ec grant aid skim milk skim milk powder buttermilk buttermilk powder intend anim feed articl regul sale skim milk powder intend anim feed articl regul articl purpos regul quot milk quot mean product obtain milk cow ad partial skim quot skim milk quot mean milk fat content protein content fatti dry extract quot skim milk powder quot mean product obtain remov water milk maximum fat content maximum moistur content protein content fatti dry extract quot buttermilk quot mean product butter manufactur obtain churn cream separ solid fat maximum fat content protein content fatti dry extract quot buttermilk powder quot mean product obtain remov water buttermilk maximum fat content maximum moistur content protein content fatti dry extract articl purpos appli regul buttermilk buttermilk powder treat skim milk skim milk powder articl quot mixtur intend manufactur compound feedingstuff quot hereinaft call quot mixtur quot mean product ingredi skim milk powder fat vitamin miner sucros anti cake agent free flow agent maximum liposolubl technic agent includ anti oxid emulsifi articl quot compound feedingstuff quot mean product kilogram finish product kilogram kilogram skim milk powder ii kilogram butter fat kilogram starch puf starch iii kilogram butter fat kilogram starch puf starch case kilogram lucern meal grass meal particl exceed micron incorpor kilogram skim milk powder particl exceed micron uniformli distribut mixtur directli feed process mix reach end user establish manufactur product quantiti skim milk powder exceed maximum quantiti kilogram refer paragraph exceed kilogram aid grant basi skim milk powder content kilogram manufactur product minimum quantiti kilogram skim milk powder refer paragraph aid minu grant skim milk powder incorpor provid skim milk powder content equal kilogram kilogram finish product articl quot denatur skim milk powder quot mean product manufactur accord formula formula kilogram skim milk powder kilogram lucern meal grass meal particl exceed micron ii kilogram starch puf pregelatinis starch formula kilogram skim milk powder kilogram lucern meal grass meal particl exceed micron ii kilogram fish meal deodoris strong smell particl exceed micron iii kilogram starch puf pregelatinis starch particl size bs standard closest maximum size laid particl product concern smaller regard equival thereto substanc ad skim milk powder uniformli distribut mixtur skim milk powder put process denatur weaken neutralis effect denatur deodoris agent chang tast smell elimin compon respons gustatori olfactori percept ad ingredi give tast smell mask fish meal chapter ii aid skim milk powder section amount aid implement condit articl aid fix eur kilogram skim milk protein content fatti dry extract eur kilogram skim milk protein content fatti dry extract eur kilogram skim milk powder protein content fatti dry extract eur kilogram skim milk powder protein content fatti dry extract case skim milk powder moistur content exceed aid reduc addit moistur articl qualifi aid skim milk powder meet condit undertak approv accord articl unalt incorpor mixtur ii unalt manufactur denatur skim milk powder benefit aid reduct price commun measur articl undertak produc mixtur compound feedingstuff denatur skim milk powder approv purpos compet agenc member state territori product take place approv grant undertak suitabl technic equip administr account method make compli regul addit requir laid member state submit inspect compet agenc case forc majeur found undertak longer meet condit laid paragraph fail compli oblig regul approv suspend period twelv month depend serious irregular end period condit laid paragraph met approv withdrawn approv reinstat month request undertak concern depth inspect member state decid impos suspens establish irregular commit deliber neglig consequ margin articl packag mixtur show inform statement list annex ii indic skim milk powder content ad miner sucros content fat content includ liposolubl technic agent indic identifi undertak refer approv number prejudic articl council direct eec compound feedingstuff pack sack close seal contain kilogram show inform statement list annex ii indic identifi undertak refer approv number skim milk powder content manufactur batch number date manufactur manufactur batch number inform legibl indel mark packag contain label affix thereto member state lai detail rule mark packag prescrib paragraph addit inform packag contain label inform commiss measur end articl articl appli compound feedingstuff lucern meal grass meal condit laid articl iii deliv tanker contain farm breed fatten concern compound feedingstuff condit laid articl articl undertak receiv aid applic authoris deliv compound feedingstuff tanker contain authoris grant compet author member state territori undertak establish deliveri place administr supervis ensur made farm breed fatten concern feedingstuff articl deliveri tanker contain take place member state produc member state proof deliveri administr supervis refer articl furnish product control copi refer articl commiss regul eec section control copi statement list annex ii import member state check consigne compli condit set paragraph articl section inspect measur articl undertak produc compound feedingstuff receiv aid record base account payment schedul fix member state includ inform quantiti milk product purchas manufactur date deliveri product date deliveri quantiti skim milk skim milk powder manufactur receiv unalt mixtur manufactur compound feedingstuff address supplier milk protein content product date manufactur compound feedingstuff quantiti manufactur particular composit percentag ingredi quantiti casein casein ad unalt mixtur date sale skim milk skim milk powder compound feedingstuff quantiti sold address consigne loss sampl return exchang skim milk skim milk powder compound feedingstuff inform list paragraph support deliveri voucher invoic member state requir undertak specif stock account show addit inform deem facilit applic regul articl order ensur complianc chapter member state carri inspect provid articl agenc respons inspect record result inspect report inform provid annex regul articl subject paragraph complianc protein moistur fat content skim milk skim milk powder incorpor inspect made latest time unalt form mixtur manufactur compound feedingstuff unalt manufactur denatur skim milk powder skim milk powder unalt mixtur directli factori premis produc inspect refer paragraph made powder leav factori premis case rule appli inspect agenc concern action ensur quantiti skim milk powder check manufactur compound feedingstuff denatur skim milk powder sack packag contain skim milk powder put bear relev inform identifi powder factori premis show date manufactur net weight protein moistur fat content powder inspect record drawn inspect agenc identifi skim milk powder state quantiti powder protein moistur fat content date manufactur ii accompani skim milk powder incorpor compound feedingstuff iii annex record refer articl articl arrang inspect skim milk skim milk powder unalt mixtur manufactur compound feedingstuff determin member state concern fulfil condit set paragraph inspect undertak concern cover composit skim milk skim milk powder unalt composit mixtur composit compound feedingstuff manufactur inspect undertak place premis relat manufactur condit establish inspect raw materi check incom outgo product sampl check record refer articl inspect unannounc made dai manufactur frequenc determin basi quantiti skim milk powder undertak frequenc account scrutinis paragraph undertak constantli skim milk skim milk powder forward manufactur programm inspect agenc member state concern arrang inspect inspect frequenc appli case manufactur compound feedingstuff subject continu inspect premis inspect refer paragraph supplement unannounc scrutini commerci document record refer articl scrutini carri month carri month frequenc inspect refer paragraph reduc dai dai manufactur articl manufactur denatur skim milk powder inspect premis dai denatur oper undertak manufactur denatur skim milk powder notifi compet agenc manufactur letter mean written telecommun factori approv number quantiti skim milk powder denatur place denatur place plan date denatur place compet agenc fix deadlin notifi manufactur date request addit inform articl subject articl refer method analys provid regul list drawn pursuant articl commiss regul ec articl skim milk powder content mixtur compound feedingstuff determin test sampl duplic accord method analysi annex iii supplement check provid articl discrep result check result spot inspect conclus absenc rennet whei proven procedur outlin annex iv starch content compound feedingstuff determin check provid articl supplement qualit analysi method set annex moistur content acid buttermilk powder determin method annex vi grass meal lucern meal content starch content fish meal content denatur skim milk powder determin laboratori analysi spot inspect provid articl articl order carri analyt test provid chapter member state obtain commiss consent set supervis system check approv undertak section payment aid articl amount aid applic dai skim milk skim milk powder process compound feedingstuff dai skim milk powder denatur case aid paid compet author design member state territori manufactur skim milk skim milk powder manufactur compound feedingstuff denatur case situat aid paid basi applic submit manufactur compound feedingstuff denatur skim milk powder hereinaft call quot beneficiari quot compet author indic address beneficiari quantiti skim milk skim milk powder aid request indic protein content applic quantiti compound feedingstuff skim milk skim milk powder refer incorpor indic relev manufactur batch number aid paid interv fix member state period cover payment applic exce month articl payment aid subject condit set paragraph result analys provid chapter check refer articl relat payment period immedi prior aid request show provis chapter compli beneficiari show satisfact compet author quantiti skim milk skim milk powder process compound feedingstuff denatur period cover aid applic case cover articl beneficiari suppli support document establish satisfact compet author compound feedingstuff deliv tanker contain farm breed fatten concern feedingstuff articl prejudic articl result analys provid chapter check refer articl show applic compli chapter previou payment period payment aid period cover current applic suspend pend result check made period question addit aid unduli paid previou period concern recov amount aid unduli paid paid skim milk skim milk powder period date inspect give rise observ date inspect indic beneficiari compli regul beneficiari request author respons inspect carri special enquiri expens quantiti shown refer subparagraph amount recov adjust articl condit laid articl met member state authoris pai advanc mean articl commiss regul eec amount equal amount aid appli subject lodg secur equal amount advanc case support document prove entitl aid suppli month payment advanc chapter iii sale skim milk powder public storag section organis particip tender procedur articl skim milk powder sold mean stand invit tender organis intervent agenc sale concern skim milk powder storag decemb stand invit tender publish offici journal european commun dai close date laid submiss tender intervent agenc draw notic invit tender indic close date address submiss tender skim milk powder hold locat warehous powder sold store quantiti sale warehous intervent agenc date list inform refer paragraph make interest parti request publish regular updat list form notic invit tender intervent agenc make arrang enabl interest parti examin sampl skim milk powder put sale expens submit tender verifi result analys refer articl commiss regul ec articl intervent agenc organis individu tender round term valid stand invit tender close date submiss tender individu round noon brussel time fourth tuesdai month tuesdai august fourth tuesdai decemb tuesdai public holidai time limit noon brussel time previou work dai articl skim milk powder sold chapter qualifi aid provid articl tender individu round submit regist letter deliv hand intervent agenc acknowledg receipt mean written telecommun tender submit intervent agenc hold skim milk powder offer made tender state address tender quantiti desir price euro tender kilogram includ nation tax charg warehous member state process compound feedingstuff denatur place warehous skim milk powder held desir substitut warehous tender valid relat tonn quantiti warehous tonn minimum quantiti offer made actual amount accompani tender written undertak compli requir process skim milk powder process compound feedingstuff denatur skim milk powder dai close date submiss tender respons individu tender round articl ii compli regul ensur compli proof provid tender lodg tender secur eur tonn member state tender submit tender round question close date submiss tender tender withdrawn close date provid articl articl purpos tender secur provid articl primari requir mean articl regul eec tender maintain close date submiss tender process secur refer articl lodg price paid section implement tender procedur articl close date refer articl member state inform commiss quantiti price offer tender quantiti skim milk powder offer sale commiss fix minimum sell price skim milk powder basi tender receiv round accord procedur laid articl regul eec price vari ag locat quantiti skim milk powder offer sale decid make award round commiss fix amount process secur kilogram skim milk powder time minimum sell price accord procedur purpos process secur ensur fulfil primari requir mean articl regul eec skim milk powder accord undertak provid articl secur lodg member state process compound feedingstuff denatur place bodi design member state articl tender reject price offer lower minimum price articl intervent agenc make award accord rule laid paragraph skim milk powder alloc basi date entri storag start oldest product total quantiti warehous design tender prejudic articl success tender tender offer highest price full quantiti alloc remaind award tender basi price tender start highest price accept tender result contract award excess quantiti skim milk powder warehous quantiti award tender question intervent agenc design warehous make quantiti set tender provid tender agre accept tender offer price skim milk powder warehous lead contract award excess quantiti award made alloc quantiti proport quantiti tender alloc lead award quantiti tonn award made draw lot articl right oblig aris connect invit tender transfer articl intervent agenc immedi inform tender outcom particip invit tender secur refer articl lodg unsuccess tender releas immedi remov skim milk powder period articl success tender pai intervent agenc amount tender quantiti withdraw lodg process tender provid articl articl amount refer articl paid secur provid articl lodg intervent agenc releas tender secur refer articl issu remov order indic quantiti respect abovement requir met warehous skim milk powder store final date remov skim milk powder final date process compound feedingstuff denatur success tender remov skim milk powder award dai close date submiss tender remov effect instal case forc majeur skim milk powder remov period laid subparagraph cost store born success tender risk dai period expir skim milk powder hand intervent agenc packag bear refer regul visibl legibl charact request interest parti intervent agenc issu copi certif indic composit product purchas provid articl regul ec addit inform provid commiss regul eec section control copi statement list annex ii section show final date process compound feedingstuff denatur chapter iv transit final provis articl regul eec eec eec eec eec ec repeal refer regul eec eec constru refer regul articl pre print packag refer articl regul eec continu june approv grant articl articl regul eec remain valid purpos appli regul regul eec continu appli quantiti skim milk powder award regul eec articl regul enter forc januari appli quantiti skim milk skim milk powder process compound feedingstuff denatur skim milk powder date regul bind entireti directli applic member state brussel decemb commiss franz fischler member commiss oj oj oj oj oj oj oj oj oj oj oj oj oj oj oj oj oj oj oj oj annex analyt test provis adopt accord council direct eec juli introduct commun method sampl analysi offici control feedingstuff oj appli sampl regul unalt skim milk powder determin moistur content protein content fat content detect product accord arrang laid nation author starch puf starch grass meal lucern meal rennet whei fish meal substanc acid whei nation author requir detect skim milk powder incorpor mixtur test addit refer determin skim milk powder content fat content includ liposolubl technic agent denatur skim milk powder test addit refer powder denatur formula determin grass meal lucern meal content starch content measur particl size grass meal lucern meal powder denatur formula determin grass meal lucern meal content starch content fish meal content measur particl size grass meal lucern meal fish meal smell test ad inert powder denatur dilut denatur dilut characterist strong smell detect compound feedingstuff determin skim milk powder content grass meal lucern meal content fat content detect starch measur particl size grass meal lucern meal check incorpor annex ii inform shown packag mixtur mezcla destinada la fabricación de pienso compuesto reglamento ce bland bestemt til fremstil af foderblanding forordn ef nr mischung zur herstellung von mischfutt verordnung nr mixtur intend manufactur compound feedingstuff regul ec mélang destiné la fabric aliment composé règlement ce miscela destinata alla fabbricazion di alimenti composti regolamento ce voor de vervaardig van mengvoed bestemd mengsel verorden nr mistura destinada ao fabrico de alimento composto regulamento ce rehuseosten valmistukseen tarkoitettu esiseo asetu ey blandn avsedd för framställn av foderblandningar förordn nr inform shown packag compound feedingstuff pienso compuesto contien lech desnatada en polvo reglamento ce foderbland med indhold af skummetmælkspulv forordn ef nr magermilchpulv enthaltend mischfutt verordnung nr compound feedingstuff skim milk powder regul ec aliment composé pour animaux conten du lait écrémé en poudr règlement ce alimento composto animali contenent latt scremato polver regolamento ce mageremelkpoed bevattend mengvoed verorden nr alimento composto para animai leit em pó desnatado regulamento ce rasvatonta maitojauhetta sisaltavä rehuseo asetu ey foderblandn innehålland skummjölkspulv förordn nr specif inform enter section control copi product deliv tanker contain pienso compuesto destinado una explotación agraria una explotación pecuaria de engord utilic lo pienso compuesto reglamento ce foderbland til brug på en landbrugsbedrift en opdrætn eller en opfedningsvirksomh forordn ef nr für landwirtschaftlich betrieb bzw aufzucht oder mastbetrieb bestimmt mischfutt verordnung nr compound feedingstuff bound farm breed fatten concern feedingstuff regul ec aliment composé pour animaux destiné un exploit agricol ou un exploit élevag ou engraiss utilisatric règlement ce alimenti composti animali destinati ad azienda agricola ad azienda dedita allevamento ingrasso che utilizzano gli alimenti composti regolamento ce mengvoed bestemd voor een dit voeder gebruikend landbouwbedrijf veeteelt veemesterijbedrijf verorden nr alimento composto para animai destinado uma exploração agrícola pecuária ou de engorda utilizadora regulamento ce maatilal jalostuskarjatilal tai lihakarjatilal tarkoitettu rehuseo asetu ey foderblandningar avsedda att använda ett jordbruksföretag eller för uppfödn eller gödning förordn nr specif inform enter section control copi case skim milk powder sold public storag debe transformars en pienso compuesto desnaturalizars reglamento ce skal forarbejd til foderblanding eller denaturer forordn ef nr zur verarbeitung zu mischfutt oder zur denaturierung verordnung nr process compound feedingstuff denatur regul ec transform en aliment composé pour animaux ou dénatur règlement ce da trasformar alimenti composti animali da denaturar regolamento ce moet tot mengvoed worden verwerkt worden gedenatureerd verorden nr para transformação em alimento composto para animai ou desnaturação regulamento ce rehuseoksiksi jalostettavaksi tai denaturoitavaksi asetu ey för bearbetn till foderblandningar eller denatur förordn nr annex iii quantit determin skim milk powder compound feedingstuff enzymat coagul para casein purpos quantit determin skim milk powder compound feedingstuff enzymat coagul para casein scope method appli compound feedingstuff skim milk powder larg quantiti buttermilk milk protein lead interfer principl method dissolv casein contain compound feedingstuff extract sodium citrat solut adjust calcium ion concentr requir level precipit para casein addit rennet para casein obtain casein nitrogen content para casein precipit determin kjeldahl method idf standard quantiti skim milk powder calcul basi minimum casein content reagent reagent analyt grade water distil water water equival puriti except rennet reagent solut free nitrogen substanc trisodium citrat dihydr solut calcium chlorid solut weigh caco analyt grade porcelain vessel suitabl size ml beaker cover distil water transfer boil water bath add slowli ml hcl solut conc hcl water solubilis carbon complet boil water bath cacl dri elimin hcl react transfer distil water ml measur flask dilut mark measur ph lower store solut refriger sodium hydroxid hydrochlor acid liquid calf rennet standard strength store refriger reagent quantit determin nitrogen kjeldahl method idf standard apparatu common laboratori apparatu includ mortar homogenis analyt balanc bench top centrifug rpm ml tube magnet stirrer mm follow ml beaker ml flask glass funnel mm diamet fast filter ashless filter diamet mm pipett nomin volum thermostat control water bath ph meter kjeldahl digest distil assembl fit ml graduat burett plastic wash bottl distil water stainless steel spatula thermomet temperatur control dry oven procedur prepar sampl grind mortar homogenis mill sampl obtain homogen mixtur dissolv milk powder separ insolubl residu weigh homogenis compound feedingstuff directli ml centrifug tube add ml trisodium citrat solut previous heat mix aid magnet stirrer minut centrifug rpm minut decant clear aqueou supernat ml beaker take care loos materi bottom carri extract residu procedur ad extract layer oil form surfac cool refriger fat solidifi remov solid layer spatula coagul casein enzym rennet stir continu add dropwis ml satur solut calcium chlorid total aqueou extract ml adjust ph solut naoh hcl place thermostat control water bath minut obtain salin balanc evid format light turbid transfer liquid centrifug tube centrifug minut order remov precipit materi transfer supernat wash sediment centrifug tube bring temperatur supernat back stir extract add dropwis ml liquid rennet coagul occur minut return sampl water bath leav temperatur minut remov sampl bath break coagulum stir centrifug minut filter supernat suitabl filter paper whatman equival retain filter paper wash precipit centrifug tube ml water approxim stir precipit centrifug minut filter supernat filter paper retain previous determin casein nitrogen wash transfer quantit precipit filter paper retain distil water transfer filter paper kjeldahl flask determin nitrogen kjeldahl method idf standard blank test blank test made regularli ashless filter paper moisten mixtur ml sodium citrat solut ml satur solut calcium chlorid ml liquid rennet wash ml distil water mineralis kjeldahl method idf standard volum acid blank test subtract volum acid titrat sampl control test test abovement procedur reagent make determin standard compound feedingstuff skim milk powder content establish collabor studi averag result duplic determin differ collabor studi express result percentag skim milk powder compound feedingstuff calcul formula gt refer graphic gt percentag para casein nitrogen factor convert determin casein percentag skim milk powder correct factor obtain regress analysi accuraci method repeat case studi duplic analysi sampl oper laboratori give differ result equival greater skim milk powder compound feedingstuff reproduc case studi sampl analys laboratori give differ result greater skim milk powder compound feedingstuff toler limit crd critic differ confid limit calcul formula iso gt refer graphic gt reproduc repeat doubl determin crd result chemic analysi differ declar content skim milk powder doubl determin consign compound feedingstuff deem compli provis regul observ addit larg percentag milk protein soya protein heat skim milk powder lead high result due precipit para casein milk addit buttermilk lead low figur due fact fat portion determin addit acid buttermilk give consider low figur due incomplet dissolv citrat solut lecithin addit lead low result incorpor high heat skim milk powder lead high figur due precipit whei protein para casein milk fast filter ashless paper annex iv determin rennet whei solid skim milk powder mixtur regul eec scope detect addit rennet whei solid skim milk powder defin articl regul eec mixtur defin articl regul eec refer intern standard iso milk milk product method sampl conform guidelin contain annex regul eec definit content rennet whei solid defin percentag mass determin procedur principl determin amount glycomacropeptid pursuant annex regul eec sampl give posit result analys glycomacropeptid reversedphas high perform liquid chromatographi procedur hplc procedur evalu result obtain refer standard sampl consist skim milk powder percentag whei powder result higher show rennet whei solid present reagent reagent recognis analyt grade water distil water water equival puriti acetonitril spectroscop hplc qualiti reagent need procedur regul eec annex regul reagent revers phase hplc trichloroacet acid solut dissolv trichloroacet acid cci cooh water make ml eluent eluent ml acetonitril ch cn ml isopropanol ch chohch ml trifluoroacet acid tfa cf cooh made water ml eluent ml acetonitril ml isopropanol ml tfa made water ml filter eluent solut prior membran filter μm pore diamet conserv column analys column flush eluent gradient subsequ flush acetonitril gradient minut column store acetonitril standard sampl skim milk powder meet requir regul eec skim milk powder adulter rennet type whei powder standard composit skim milk powder adulter rennet type whei powder standard composit apparatu apparatu need procedur regul eec annex regul apparatu revers phase hplc analyt balanc centrifug capabl attain centrifug forc fit stopper centrifug tube ml mechan shaker provis shake magnet stirrer glass funnel diamet cm filter paper medium filtrat diamet cm glass filtrat equip μm pore diamet membran filter graduat pipett allow deliveri ml iso class iso system capabl deliveri ml minut thermostat waterbath set hplc equip consist binari gradient pump system injector hand automat μl capac column dupont protein cm equival wide pore silica base reversedphas column thermostat column oven set variabl wavelength uv detector permit measur nm higher wavelength nm sensit integr capabl peak height measur note oper column room temperatur provid room temperatur fluctuat variat retent time gmpa take place sampl intern standard iso milk milk product method sampl conform guidelin contain annex regul eec store sampl condit preclud deterior chang composit procedur prepar test sampl transfer powder contain capac volum powder fit airtight lid close contain immedi mix milk powder mean repeat invers contain test portion weigh test sampl centrifug tube suitabl stopper flask ml remov fat protein add warm water test portion dissolv powder shake minut minut case acid buttermilk mechan shaker place tube waterbath equilibr add ml trichloroacet acid solut constantli minut stir vigor aid magnet stirrer place tube waterbath leav minut centrifug minut filter paper discard ml filtrat chromatograph determin perform hplc analysi regul eec annex neg result obtain sampl analys rennet whei solid detect amount case posit result revers phase hplc procedur appli presenc acid buttermilk powder give rise fals posit result revers phase hplc method exclud possibl revers phase hplc analysi carri gradient condit optimis retent time minut gmpa optim gradient system dead volum ml volum point solvent volum injector loop inclus gradient system lower dead volum ml minut optim retent time solut standard sampl rennet whei inject μl supernat filtrat hplc apparatu oper scout gradient condit tabl tabl scout gradient condit optimis chromatographi gt tabl gt comparison chromatogram reveal locat peak gmpa formula initi solvent composit normal gradient calcul gt refer graphic gt gt refer graphic gt rtgmpa retent time gmpa scout gradient initi scout gradient midpoint minu initi normal gradient midpoint time scout gradient requir retent time gmpa ratio slope scout normal gradient initi minu minut scout gradient run time scout gradient solut test sampl inject μl accur measur supernat filtrat hplc apparatu oper flow rate ml eluent solut minut composit eluent start analysi obtain close immedi inject linear gradient start result higher percentag minut subsequ linear gradient start bring eluent composit minut composit maintain minut composit chang linear gradient minut initi composit depend intern volum pump system inject made minut reach initi condit remark retent time glycomacropeptid minut achiev vari initi end condit gradient differ initi end condit gradient remain eluent degass suffici remain degass essenti proper function gradient pump system standard deviat retent time gmp peak smaller minut sampl refer sampl inject calcul respons factor result chromatograph analysi test sampl obtain form chromatogram gmp peak identifi retent time minut integr automat calcul peak height gmp peak baselin locat check chromatogram analysi integr repeat baselin incorrectli locat essenti examin appear chromatogram prior quantit interpret order detect abnorm due malfunct apparatu column origin natur sampl analys doubt repeat analysi calibr appli procedur point point standard sampl freshli prepar solut gmp degrad trichloroacet acid environ room temperatur solut remain stabl hour case long seri analys cool sampl trai automat injector desir note omit initi condit previou analys chromatogram refer sampl analog figur figur gmpa peak preced small peak essenti obtain similar separ prior chromatograph determin sampl inject μl standard sampl rennet whei chromatogram show peak retent time gmpa peak determin respons factor inject volum filtrat sampl express result method calcul formula calcul respons factor gt refer graphic gt respons factor gmp peak height gmp peak quantiti whei standard sampl calcul percentag rennet whei powder sampl gt refer graphic gt percentag rennet whei sampl respons factor gmp peak height gmp peak sampl greater differ retent time standard sampl smaller minut rennet whei solid present accuraci procedur repeat differ result determin carri simultan rapid success analyst apparatu ident test materi exce reproduc determin linear rennet whei linear relationship obtain coeffici correl amp gt interpret whei consid present result obtain higher retent time gmp peak differ minut standard sampl limit fix agreement provis point annex regul eec gt pic file quot en ep quot gt rennet type whei powder standard composit adulter skim milk powder nizo kernhemseweg po box nl ba ed powder give equival result nizo powder annex qualit determin starch skim milk powder denatur milk powder compound feedingstuff scope method detect starch isu tracer denatur milk powder limit detect method approxim starch sampl principl reaction base iodometri fixat colloid free iodin aqueou solut absorpt starch micel colour format reagent iodin solut gt tabl gt apparatu analyt balanc water bath test tube mm mm procedur weight sampl transfer test tube add ml distil water shake order dispers sampl place boil water bath leav minut remov bath cool room temperatur add ml iodin solut shake observ result colour express result blue colour presenc nativ starch sampl sampl modifi starch colour blue remark colour intens colour microscop appear starch vari depend origin nativ starch maiz potato type modifi starch present sampl presenc modifi starch colour produc turn violet red brown degre modif crystallin structur nativ starch annex vi determin moistur acid buttermilk powder scope determin moistur content acid buttermilk powder intend anim feedingstuff principl sampl dri vacuum loss mass determin weigh apparatu analyt balanc dry contain corrod metal glass lid ensur airtight closur work surfac allow test sampl spread cm adjust electr heat vacuum oven fit oil pump mechan introduc hot dri air dry agent calcium oxid desicc effici dry agent dry oven ventil thermostat control procedur heat contain lid oven hour place lid contain immedi transfer desicc cool room temperatur weigh nearest mg weigh contain lid nearest mg weight weight contain nearest mg sampl spread evenli place conain lid vacuum oven preheat prevent oven temperatur fall unduli introduc contain rapidli bring pressur torr kpa leav dry hour pressur current hot dry air dry agent sampl instanc disconnect vacuum pump prescrib pressur reach reckon dry time moment oven temperatur return carefulli bring oven back atmospher pressur open oven place lid contain immedi remov contain oven leav cool minut desicc weigh nearest mg dry addit minut vacuum oven reweigh differ weigh exce moistur calcul gt refer graphic gt initi mass gramm test sampl mass gramm dry test sampl precis repeat limit differ result determin carri shortest feasibl time interv oper apparatu ident test materi exce water acid buttermilk powder reproduc limit differ result determin carri oper laboratori apparatu ident test materi exce water acid buttermilk powder sourc precis data precis data determin experi conduct involv laboratori sampl blind duplic