commiss direct octob method analysi edibl casein casein eec commiss european commun regard treati establish european econom commun regard council direct eec juli approxim law member state relat lactoprotein casein casein intend human consumpt articl thereof articl direct eec requir commun method analysi determin check composit edibl casein casein adopt initi seri method respect studi complet measur provid direct accord opinion stand committe foodstuff adopt direct articl member state measur ensur analys verif criteria set annex carri accord method annex ii articl member state bring forc law regul administr provis compli direct latest forthwith inform commiss thereof articl direct address member state brussel octob commiss cockfield vice presid oj annex scope commun method analysi direct edibl casein casein gener provis ii determin moistur acid casein method annex ii rennet casein method annex ii casein method annex ii iii determin protein content acid casein method annex ii rennet casein method annex ii casein method annex ii iv determin titrat acid acid casein method annex ii determin ash includ acid casein method annex ii rennet casein method annex ii vi determin ph casein method annex ii annex ii method analysi relat composit edibl casein casein gener provis prepar analysi sampl gener mass sampl present laboratori analysi gram prepar sampl analysi laboratori mix break lump laboratori sampl repeatedli shake invert contain transfer laboratori sampl air tight contain suffici capac volum sampl oper carri transfer repres portion sampl gram mix laboratori sampl test siev gram portion complet complet pass weight siev determin sampl prepar grind gram portion grind devic satisfi siev criterion immedi transfer siev sampl air tight contain suffici capac volum sampl mix repeat shake invert oper precaut avoid chang moistur content product test sampl prepar determin proceed contain sampl air tight moistur tight contain reagent water mention made water solut dilut wash purpos distil water deminer water equival puriti refer made solut dilut indic solut water dilut water meant chemic chemic recogn analyt reagent qualiti equip list equip list equip item special item specif analyt balanc analyt balanc mean balanc capabl weigh mg test siev test siev fit lid diamet mm construct wire cloth nomin apertur size mm apertur toler wire diamet permit iso test siev technic requir test part metal wire cloth iso siev fit receiv grind devic grind laboratori sampl develop undu heat loss absorpt moistur hammer mill express result result result state analyt report obtain determin satisfi repeat criterion method calcul percentag result calcul percentag mass sampl test report test report identifi method analysi result obtain addit mention detail procedur method analysi option circumst influenc result obtain test report give inform complet identif sampl method determin moistur content scope field applic method determin moistur content acid casein rennet casein casein definit moistur content casein casein loss mass determin method principl residu mass test portion determin dry atmospher pressur oven constant mass loss mass calcul percentag mass sampl apparatu analyt balanc dish flat bottom materi corrod condit test nickel aluminium stainless steel glass dish lid fit tightli readili remov suitabl dimens diamet mm depth mm atmospher pressur dry oven ventil thermostat control temperatur regul temperatur uniform oven desicc freshli activ silica gel water content indic equival desicc suitabl devic handl dish laboratori tong procedur prepar test sampl section gener provis method determin protein content scope field applic method determin protein content acid casein rennet casein casein ammonium casein ammonium nitrogen protein compound definit protein content nitrogen content determin method multipli express percentag mass principl test portion digest mixtur potassium sulphat sulphur acid presenc copper ii sulphat catalyst convert organ nitrogen ammoniac nitrogen ammonia distil absorb boric acid solut titrat standard hydrochlor acid solut nitrogen content convert protein content multipli reagent sulphur acid concentr ml potassium sulphat anhydr copper ii sulphat pentahydr cuso sucros boric acid solut sodium hydroxid concentr aqueou solut carbon free hydrochlor acid mol mix indic mix equal volum solut methyl red ethanol solut methylen blue ethanol apparatu analyt balanc kjeldahl flask ml capac digest apparatu hold kjeldahl flask inclin posit heat devic heat part flask surfac liquid content condens straight tube outlet tube safeti bulb connect lower end condens ground glass joint rubber tube rubber tube glass end splash head connect kjeldahl flask condens soft close fit rubber stopper conic flask ml capac graduat cylind ml ml capac burett ml capac graduat ml boil aid digest small piec hard porcelain glass bead distil freshli calcin piec pumic procedur prepar test sampl section gener provis test presenc ammoniac nitrogen presenc ammonium casein ammonium compound suspect carri test add gram sampl small conic flask ml water mg magnesium oxid rins magnesium oxid adher wall close flask cork stopper insert piec moisten red litmu paper stopper neck flask mix content flask carefulli heat flask water bath litmu paper colour blue minut ammonia present method applic section blank test time determin nitrogen content sampl perform blank determin gram sucros test portion apparatu quantiti reagent procedur titrat blank determin exce ml mol acid reagent check impur reagent reagent purifi replac test portion transfer kjeldahl flask gram test sampl weigh nearest mg determin transfer flask piec porcelain glass bead gram anhydr potassium sulphat add copper ii sulphat wash neck flask water add ml concentr sulphur acid mix content flask heat gentli digest apparatu froth ceas boil gentli solut clear pale green blue colour persist heat swirl flask occasion continu boil regul heat condens vapour middl flask neck continu heat minut avoid local overh cool room temperatur carefulli add ml water piec pumic mix cool transfer conic flask ml boric acid solut drop indic mix place conic flask condens tip outlet tube immers boric acid solut graduat cylind add kjeldahl flask ml sodium hydroxid solut oper hold flask inclin posit sodium hydroxid solut run side flask form bottom layer immedi connect kjeldahl flask condens mean splash head gentli rotat kjeldahl flask mix content boil gentli avoid froth continu distil ml distil collect approxim minut distil temperatur minut end distil lower conic flask tip outlet tube longer immers acid solut rins tip water stop heat remov outlet tube rins outer wall water collect wash conic flask titrat distil conic flask standard volumetr hydrochlor acid solut express result formula method calcul protein content sampl express percentag mass volum millilitr standard volumetr hydrochlor acid solut determin volum millilitr standard volumetr hydrochlor acid solut blank test strength standard volumetr hydrochlor acid solut mol mass gram test portion calcul protein content nearest repeat differ result determin carri simultan rapid success sampl analyst condit exce gram protein gram product repeat interv achiev time method correctli carri method determin titrat acid scope field applic method determin titrat acid acid casein definit titrat acid acid casein volum millilitr mol standard sodium hydroxid solut requir neutral aqueou extract gram product principl aqueou extract sampl obtain filter filtrat titrat standard sodium hydroxid phenolphtalein indic reagent water method procedur prepar reagent freed carbon dioxid boil minut sodium hydroxid solut mol phenolphtalein indic solut ethanol neutral indic apparatu analyt balanc conic flask ml capac ground neck fit ground glass stopper mark pipett ml capac pipett suitabl measur ml indic solut conic flask ml capac measur cylind ml capac burett graduat ml water bath capabl control temperatur filter procedur prepar test sampl section gener provis test portion weigh gram test sampl nearest mg transfer conic flask determin ml measur cylind add ml freshli boil cool water previous heat stopper flask mix swirl place water bath minut shake flask interv minut filter cool filtrat filtrat clear transfer ml cool filtrat conic flask pipett add ml phenolphtalein indic solut pipett titrat standard volumetr sodium hydroxid solut appear faint pink colour persist second determin record volum nearest ml express result formula method calcul titrat acid acid casein volum millilitr standard volumetr sodium hydroxid solut strength standard volumetr sodium hydroxid solut mol mass gram test portion calcul titrat acid decim place repeat differ result determin carri simultan rapid success sampl analyst condit exce ml mol sodium hydroxid gram product repeat interv achiev time method correctli carri method determin ash includ scope field applic method determin ash includ content acid casein definit ash includ content content ash determin method principl portion sampl inciner presenc magnesium acet bind phosphoru organ origin final ash calcul weigh residu subtract mass ash origin magnesium acet reagent magnesium acet tetrahydr solut dissolv gram magnesium acet tetrahydr mg ch water make litr water apparatu analyt balanc mark pipett ml silica platinum dish mm diamet mm deep dry oven capabl control electr furnac capabl control boil water bath desicc freshli activ silica gel water content indic equival desicc procedur prepar test sampl section gener provis prepar dish heat dish electr furnac control minut dish cool place desicc temperatur balanc room weigh nearest mg test portion weigh nearest mg approxim gram test sampl directli prepar dish determin pipett add dish ml magnesium acet solut wet test portion stand minut prepar dish add pipett ml magnesium acet solut evapor content dish dryness boil water bath place dish oven control minut heat dish content low flame hot plate lamp test portion complet char take care burst flame transfer dish electr furnac control heat hour carbon disappear dish dish cool place desicc temperatur balanc room weigh nearest mg repeat oper heat approxim minut electr furnac cool weigh mass remain constant mg begin increas record minimum mass express result method calcul content ash includ sampl percentag mass mass gram test portion mass gram dish residu mass gram prepar dish mass gram dish residu mass gram prepar dish calcul final result nearest repeat differ result determin carri simultan rapid success sampl analyst condit exce gram gram product repeat interv achiev time method correctli carri method determin ash includ scope field applic method determin ash includ content rennet casein definit ash includ content content ash determin method principl portion sampl inciner constant mass residu determin weigh calcul percentag mass sampl apparatu analyt balanc silica platinum dish mm diamet mm deep electr furnac air circul capabl control desicc freshli activ silica gel water content indic equival desicc procedur prepar test sampl section gener provis prepar dish heat dish electr furnac control minut dish cool place desicc temperatur balanc room weigh nearest mg test portion weigh nearest mg approxim gram test sampl directli prepar dish determin heat dish content low flame hot plate lamp test portion complet char take care burst flame transfer dish electr furnac control heat hour carbon disappear dish dish cool place desicc temperatur balanc room weigh nearest mg repeat oper heat approxim minut electr furnac cool weigh mass remain constant mg begin increas record minimum mass express result method calcul formula ash content sampl includ percentag mass mass gram test portion mass gram dish residu mass gram prepar dish calcul final result nearest repeat differ result determin carri simultan rapid success sampl analyst condit exce gram ash gram product repeat interv achiev time method correctli carri method determin ph scope field applic method determin ph casein definit ph casein ph aqueou solut casein determin method principl electrometr determin ph aqueou solut casein ph meter reagent water prepar reagent procedur recent distil water protect carbon dioxid absorpt buffer solut calibr ph meter standard buffer solut ph valu decim place bracket ph sampl test phtalat buffer solut ph approxim borax buffer solut ph approxim apparatu balanc accuraci gram ph meter minimum sensit ph unit suitabl calibr elecrod glass electrod calomel refer electrod thermomet accuraci conic flask capac ml fit ground glass stopper beaker capac ml mixer beaker mixer ml capac procedur prepar test sampl section gener provis determin calibr ph meter adjust temperatur buffer solut calibr ph meter accord manufactur instruct note calibr carri flask stand mintu seri sampl test check calibr ph meter standard buffer solut minut prepar test solut transfer beaker ml water add gram test sampl mix mixer second stand minut cover watch glass measur ph pour ml solut beaker immedi determin ph liquid ph meter rins glass electrod carefulli water measur ph express result record ph record ph aqueou solut casein read dial ph meter decim place repeat differ result determin carri simultan rapid success sampl analyst condit exce ph unit repeat interv achiev time method correctli carri transfer flask piec porcelain glass bead gram anhydr potassium sulphat add copper ii sulphat wash neck flask water add ml concentr sulphur acid mix content flask heat gentli digest apparatu froth ceas boil gentli solut clear pale green blue colour persist heat swirl flask occasion continu boil regul heat condens vapour middl flask neck continu heat minut avoid local overh cool room temperatur carefulli add ml water piec pumic mix cool transfer conic flask ml boric acid solut drop indic mix place conic flask condens tip outlet tube immers boric acid solut graduat cylind add kjeldahl flask ml sodium hydroxid solut oper hold flask inclin posit sodium hydroxid solut run side flask form bottom layer immedi connect kjeldahl flask condens mean splash head gentli rotat kjeldahl flask mix content boil gentli avoid froth continu distil ml distil collect approxim minut distil temperatur minut end distil lower conic flask tip outlet tube longer immers acid solut rins tip water stop heat remov outlet tube rins outer wall water collect wash conic flask titrat distil conic flask standard volumetr hydrochlor acid solut express result formula method calcul protein content sampl express percentag mass volum millilitr standard volumetr hydrochlor acid solut determin volum millilitr standard volumetr hydrochlor acid solut blank test strength standard volumetr hydrochlor acid solut mol mass gram test portion calcul protein content nearest repeat differ result determin carri simultan rapid success sampl analyst condit exce gram protein gram product repeat interv achiev time method correctli carri method determin titrat acid scope field applic method determin titrat acid acid casein definit titrat acid acid casein volum millilitr mol standard sodium hydroxid solut requir neutral aqueou extract gram product principl aqueou extract sampl obtain filter filtrat titrat standard sodium hydroxid phenolphtalein indic reagent water method procedur prepar reagent freed carbon dioxid boil minut sodium hydroxid solut mol phenolphtalein indic solut ethanol neutral indic apparatu analyt balanc conic flask ml capac ground neck fit ground glass stopper mark pipett ml capac pipett suitabl measur ml indic solut conic flask ml capac measur cylind ml capac burett graduat ml water bath capabl control temperatur filter procedur prepar test sampl section gener provis test portion weigh gram test sampl nearest mg transfer conic flask determin ml measur cylind add ml freshli boil cool water previous heat stopper flask mix swirl place water bath minut shake flask interv minut filter cool filtrat filtrat clear transfer ml cool filtrat conic flask pipett add ml phenolphtalein indic solut pipett titrat standard volumetr sodium hydroxid solut appear faint pink colour persist second determin record volum nearest ml express result formula method calcul titrat acid acid casein volum millilitr standard volumetr sodium hydroxid solut strength standard volumetr sodium hydroxid solut mol mass gram test portion calcul titrat acid decim place repeat differ result determin carri simultan rapid success sampl analyst condit exce ml mol sodium hydroxid gram product repeat interv achiev time method correctli carri method determin ash includ scope field applic method determin ash includ content acid casein definit ash includ content content ash determin method principl portion sampl inciner presenc magnesium acet bind phosphoru organ origin final ash calcul weigh residu subtract mass ash origin magnesium acet reagent magnesium acet tetrahydr solut dissolv gram magnesium acet tetrahydr mg ch water make litr water apparatu analyt balanc mark pipett ml silica platinum dish mm diamet mm deep dry oven capabl control electr furnac capabl control boil water bath desicc freshli activ silica gel water content indic equival desicc procedur prepar test sampl section gener provis prepar dish heat dish electr furnac control minut dish cool place desicc temperatur balanc room weigh nearest mg test portion weigh nearest mg approxim gram test sampl directli prepar dish determin pipett add dish ml magnesium acet solut wet test portion stand minut prepar dish add pipett ml magnesium acet solut evapor content dish dryness boil water bath place dish oven control minut heat dish content low flame hot plate lamp test portion complet char take care burst flame transfer dish electr furnac control heat hour carbon disappear dish dish cool place desicc temperatur balanc room weigh nearest mg repeat oper heat approxim minut electr furnac cool weigh mass remain constant mg begin increas record minimum mass express result method calcul content ash includ sampl percentag mass mass gram test portion mass gram dish residu mass gram prepar dish mass gram dish residu mass gram prepar dish calcul final result nearest repeat differ result determin carri simultan rapid success sampl analyst condit exce gram gram product repeat interv achiev time method correctli carri method determin ash includ scope field applic method determin ash includ content rennet casein definit ash includ content content ash determin method principl portion sampl inciner constant mass residu determin weigh calcul percentag mass sampl apparatu analyt balanc silica platinum dish mm diamet mm deep electr furnac air circul capabl control desicc freshli activ silica gel water content indic equival desicc procedur prepar test sampl section gener provis prepar dish heat dish electr furnac control minut dish cool place desicc temperatur balanc room weigh nearest mg test portion weigh nearest mg approxim gram test sampl directli prepar dish determin heat dish content low flame hot plate lamp test portion complet char take care burst flame transfer dish electr furnac control heat hour carbon disappear dish dish cool place desicc temperatur balanc room weigh nearest mg repeat oper heat approxim minut electr furnac cool weigh mass remain constant mg begin increas record minimum mass express result method calcul formula ash content sampl includ percentag mass mass gram test portion mass gram dish residu mass gram prepar dish calcul final result nearest repeat differ result determin carri simultan rapid success sampl analyst condit exce gram ash gram product repeat interv achiev time method correctli carri method determin ph scope field applic method determin ph casein definit ph casein ph aqueou solut casein determin method principl electrometr determin ph aqueou solut casein ph meter reagent water prepar reagent procedur recent distil water protect carbon dioxid absorpt buffer solut calibr ph meter standard buffer solut ph valu decim place bracket ph sampl test phtalat buffer solut ph approxim borax buffer solut ph approxim apparatu balanc accuraci gram ph meter minimum sensit ph unit suitabl calibr elecrod glass electrod calomel refer electrod thermomet accuraci conic flask capac ml fit ground glass stopper beaker capac ml mixer beaker mixer ml capac procedur prepar test sampl section gener provis determin calibr ph meter adjust temperatur buffer solut calibr ph meter accord manufactur instruct note calibr carri flask stand mintu seri sampl test check calibr ph meter standard buffer solut minut prepar test solut transfer beaker ml water add gram test sampl mix mixer second stand minut cover watch glass measur ph pour ml solut beaker immedi determin ph liquid ph meter rins glass electrod carefulli water measur ph express result record ph record ph aqueou solut casein read dial ph meter decim place repeat differ result determin carri simultan rapid success sampl analyst condit exce ph unit repeat interv achiev time method correctli carri