commiss direct juli lai commun method analysi verifi addit foodstuff satisfi criteria puriti eec commiss european commun regard treati establish european econom commun regard council direct octob approxim law member state colour matter author foodstuff intend human consumpt amend direct eec articl thereof regard council direct eec novemb approxim law member state preserv author foodstuff intend human consumpt amend direct eec articl thereof regard council direct eec juli approxim law member state antioxid author foodstuff intend human consumpt amend direct eec articl thereof provis lai commun method analysi establish verifi addit satisfi gener specif criteria puriti seri method studi complet adopt measur provid direct accord opinion stand committe foodstuff adopt direct articl member state prescrib analys verifi addit foodstuff satisfi gener specif criteria oj oj oj oj oj oj puriti carri method annex ii scope laid annex articl member state bring forc law regul administr provis compli direct februari forthwith inform commiss thereof articl direct address member state brussel juli commiss karl heinz narj member commiss annex scope commun method analysi verifi addit foodstuff meet puriti criteria introduct ii colour matter ii determin substanc extract diethyl ether water solubl sulphon organ colour matter foodstuff annex ii method iii preserv iii determin formic acid format oxidiz impur acet acid potassium acet sodium diacet calcium acet annex ii method iii determin volatil substanc propion acid annex ii method iii determin loss mass dry sodium nitrit annex ii method iii limit test salicyl acid ethyl hydroxybenzo ethyl hydroxybenzo sodium salt propyl hydroxybenzo propyl hydroxybenzo sodium salt methyl hydroxybenzo methyl hydroxybenzo sodium salt annex ii method iii determin free acet acid sodium diacet annex ii method iii determin sodium acet sodium diacet annex ii method iii limit test determin aldehyd sorbic acid sodium potassium calcium sorbat propion acid annex ii method iv antioxid iv determin number peroxid group lecithin annex ii method iv determin toluen insolubl substanc lecithin annex ii method iv limit test reduc substanc sodium potassium calcium lactat annex ii method iv determin volatil acid orthophosphor acid annex ii method iv limit test nitrat orthophosphor acid annex ii method iv determin water insolubl substanc mono di tri sodium orthophosph mono di tri potassium orthophosph ii iii ii iii annex ii method gener determin ph food addit annex ii method annex ii method analysi relat criteria puriti food addit introduct prepar analysi sampl gener mass laboratori sampl intend analysi larger quantiti requir specif determin sampl prepar sampl made homogen prior analysi preserv prepar sampl air tight moistur tight contain store deterior prevent reagent water mention made water solut dilut wash purpos distil water deminer water equival puriti intend refer made quot solut quot quot dilut quot indic reagent aqueou solut intend chemic chemic analyt reagent qualiti equip list equip list equip item special item specif analyt balanc analyt balanc mean balanc sensit mg greater express result result result state offici analysi report determin repeat satisfactori calcul percentag state result express percentag mass origin sampl receiv laboratori number signific figur number signific figur result express govern precis method method determin substanc extract diethyl ether water solubl sulphon organ colour matter intend foodstuff scope field applic method determin substanc extract diethyl ether water solubl sulphon organ colour matter mix support definit substanc extract diethyl ether content materi determin method principl extract colour matter diethyl ether weigh extract residu evapor ether reagent diethyl ether dry peroxid free dri aid freshli calcin calcium chlorid apparatu soxhlet apparatu flask desicc freshli activ silica gel equival desicc water content indic analyt balanc oven thermostat control ºc procedur accur weigh nearest mg sampl colour matter piec filter paper fold paper put paper thimbl close fat free cotton wool extract hour diethyl ether soxhlet extract apparatu evapor ether low temperatur place soxhlet flask previous weigh residu oven ºc minut dry transfer flask desicc cover loos fit lid cool weigh flask residu repeat dry weigh success weigh differ mg increas mass occur lowest record read calcul express result formula method calcul content substanc extract ether percentag sampl gt pic file quot quot gt mass gram residu evapor initi mass gram sampl repeat differ result determin carri simultan rapid success sampl analyst condit exce mg sampl method determin formic acid format oxidiz impur acet acid potassium acet sodium diacet calcium acet scope field applic method determin formic acid format oxidiz impur express formic acid acet acid potassium acet sodium diacet calcium acet definit formic acid format oxidiz impur content content formic acid format oxidiz impur determin method principl solut sampl treat excess standard potassium permangan alkalin condit form manganes dioxid manganes dioxid excess potassium permangan determin iodometr acid condit concentr oxidiz impur calcul express formic acid reagent gt pic file quot quot gt apparatu water bath boil analyt balanc procedur test sampl free acid accur weigh nearest mg sampl dilut ml water add solut anhydr sodium carbon ml water sampl salt accur weigh nearest mg sampl dissolv ml water add anhydr sodium carbon shake dissolv add ml mol potassium permangan heat boil water bath minut cool mixtur add ml dilut sulphur acid potassium iodid swirl mixtur precipit manganes dioxid redissolv titrat mol sodium thiosulph solut pale yellow colour add drop starch solut continu titrat solut colourless express result formula method calcul percentag formic acid format oxidiz impur express formic acid gt pic file quot quot gt molar potassium permangan molar sodium thiosulph initi mass gram sampl volum millilitr mol sodium thiosulph titrat repeat differ result determin carri simultan rapid success sampl analyst condit exce mg sampl note volum ml mol sodium thiosulph equival formic acid sampl format present volum requir ml formic acid present insuffici excess potassium permangan fix minimum volum ml obtain case repeat determin smaller sampl weight method determin volatil substanc propion acid scope field applic method determin volatil substanc propion acid definit content volatil materi propion acid content volatil materi determin method principl sampl evapor dri ºc residu determin gravimetr apparatu evapor vessel silica platinum suffici size sampl oven electr heat thermostat control ºc analyt balanc water bath boil desicc freshli activ silica gel equival desicc water content indic procedur weigh nearest sampl propion acid previous dri weigh vessel evapor boil water bath fume cupboard propion acid evapor place oven ºc hour place desicc cool weigh repeat heat cool weigh oper differ success weigh mg increas mass occur lowest record read calcul express result formula method calcul volatil matter content calcul percentag sampl gt pic file quot quot gt mass gram residu evapor mass gram sampl repeat differ result determin carri simultan rapid success sampl analyst condit exce mg sampl method determin loss mass dry sodium nitrit scope field applic method determin loss mass dry sodium nitrit definit moistur content sodium nitrit loss mass dry determin method principl loss mass dry obtain heat oven ºc weigh calcul loss mass apparatu oven electr heat thermostat control ºc weigh dish flat bottom glass diamet mm depth mm loos fit lid desicc freshli activ silica gel equival desicc water content indic analyt balanc procedur remov lid weigh dish heat dish lid oven ºc hour replac lid place dish lid desicc cool room temperatur weigh cover dish nearest mg accur weigh nearest mg approxim sampl cover dish remov lid place dish lid oven hour ºc replac lid cover dish cool room temperatur desicc weigh nearest mg repeat heat cool weigh differ success weight mg increas mass occur lowest record read calcul express result formula method calcul loss mass dry calcul percentag mass sampl gt pic file quot quot gt mass gram dish mass gram dish sampl dry mass gram dish sampl dry repeat differ result determin carri simultan rapid success sampl analyst condit exce mg sampl method limit test salicyl acid ethyl hydroxybenzo ethyl hydroxybenzo sodium salt propyl hydroxybenzo propyl hydroxybenzo sodium salt methyl hydroxybenzo methyl hydroxybenzo sodium salt scope field applic method detect salicyl acid ethyl hydroxybenzo propyl hydroxybenzo methyl hydroxybenzo sodium salt definit detect limit test concentr salicyl acid limit test result determin method principl violet colour produc reaction ammonium iron iii sulphat solut sampl intens compar produc refer solut reagent ammonium iron iii sulphat solut prepar dissolv ammonium iron iii sulphat dodecahydr ml water add ml nitric acid dilut ml water ethanol salicyl acid solut sulphur acid mol apparatu nessler cylind graduat ml total volum approxim ml procedur ethyl propyl methyl hydroxybenzo sampl weigh nearest mg sampl dissolv ml ethanol transfer solut graduat nessler cylind dilut ml water stir add ml ammonium iron iii sulphat solut stir stand minut prepar comparison solut time repeat replac sampl ml salicyl acid solut compar colour sampl solut appear comparison solut sodium salt ethyl propyl methyl hydroxybenzo sampl repeat acidifi ph mol sulphur acid dilut ml repeat repeat express result limit test interpret reddish violet colour appear sampl solut tube intens appear comparison solut tube test posit sampl salicyl acid sensit limit detect test mg salicyl acid sampl observ result limit test carri simultan rapid success sampl analyst condit ident method determin free acet acid sodium diacet scope field applic method determin acet acid sodium diacet definit acet acid content content acet acid determin method principl direct titrat acet acid sampl standard sodium hydroxid solut phenolphthalein indic reagent phenolphthalein solut ethanol sodium hydroxid mol apparatu analyt balanc procedur weigh nearest mg test sampl dissolv ml water add drop phenolphthalein indic solut titrat mol sodium hydroxid red tint persist second express result formula method calcul acet acid content percentag sampl mass gt pic file quot quot gt volum millilitr sodium hydroxid requir concentr sodium hydroxid solut mol initi mass gram sampl repeat differ result determin carri simultan rapid success sampl analyst condit exce mg sampl comment volum ml obtain sampl acet acid titrat mol sodium hydroxid method determin sodium acet sodium diacet scope field applic method determin sodium acet water express sodium acet sodium diacet definit sodium acet content content sodium acet water express sodium acet determin method principl sampl dissolv glacial acet acid titrat standard perchlor acid crystal violet indic reagent gt pic file quot quot gt gt pic file quot quot gt apparatu analyt balanc procedur weigh nearest mg sampl dissolv ml glacial acet acid add drop crystal violet indic solut titrat pale green end point standard mol perchlor acid express result formula method calcul sodium acet content defin section definit express percentag weight sampl formula gt pic file quot quot gt volum millilitr standard perchlor acid molar perchlor acid solut initi mass gram sampl repeat differ result determin carri simultan rapid success sampl analyst condit exce sampl observ reagent method toxic explos care handl method limit test aldehyd sorbic acid sodium potassium calcium sorbat propion acid scope field applic method detect aldehyd express formaldehyd sorbic aid sodium potassium calcium sorbat propion acid definit detect limit test concentr aldehyd limit test result determin method principl aldehyd test solut formaldehyd comparison solut react schiff reagent produc red colour complex intens compar reagent formaldehyd standard solut mg ml prepar dilut concentr formaldehyd solut mg ml schiff reagent procedur weigh nearest mg sampl add ml water shake filter solut treat ml filtrat sampl solut ml schiff reagent time treat ml formaldehyd comparison solut ml schiff reagent compar colour sampl solut appear comparison solut express result limit test interpret red colour appear sampl solut tube intens appear comparison solut tube test posit sampl aldehyd express formaldehyd sensit limit detect test mg formaldehyd sampl observ result limit test carri simultan rapid success sampl analyst condit ident method determin peroxid number lecithin scope field applic method determin peroxid number lecithin definit peroxid number lecithin result obtain determin method principl oxid potassium iodid peroxid lecithin titrat iodin liber standard sodium thiosulph solut reagent acet acid glacial chloroform potassium iodid sodium thiosulph mol mol starch solut approxim apparatu analyt balanc apparatu shown figur consist round bottom flask ml reflux condens glass tube mm long mm intern diamet fit ground glass joint micro beaker extern dimens mm diamet mm height procedur place ml glacial acet acid ml chloroform ml flask fit glass tube reflux condens gentli boil mixtur minut expel dissolv air dissolv potassium iodid ml water add solut mixtur flask take care boil interrupt yellow colour appear stage determin reject repeat fresh reagent accur weigh nearest mg sampl minut boil add weigh sampl content flask take care boil remain continu purpos sampl contain micro beaker lower glass tube glass rod bottom suitabl shape shown diagram condens remov short time continu boil minut stop heat immedi disconnect condens quickli add ml water glass tube remov glass tube cool flask room temperatur water tap titrat sodium thiosulph mol mol aqueou layer pale yellow add ml starch solut continu titrat blue colour discharg shake flask titrat ensur complet extract iodin aqueou layer obtain blank titrat repeat complet procedur ad sampl express result formula method calcul peroxid number sampl milliequival kilogram gt pic file quot quot gt volum millilitr thiosulph solut requir titrat sampl volum millilitr thiosulph solut requir titrat blank concentr sodium thiosulph solut mol initi mass gram sampl repeat differ result determin carri simultan rapid success sampl analyst condit exce express peroxid number milliequival kilogram sampl note choic concentr sodium thiosulph depend anticip titrat ml mol sodium thiosulph requir repeat determin mol sodium thiosulph determin carri strong light gt pic file quot quot gt method determin toluen insolubl matter lecithin scope field applic method determin toluen insolubl matter lecithin definit toluen insolubl matter content content toluen insolubl matter determin method principl sampl dissolv toluen filter residu dri weigh reagent toluen apparatu sinter glass crucibl ml capac equival poros dry oven electr heat thermostat control ºc water bath oper temperatur exceed ºc desicc freshli activ silica gel equival desicc water content indic conic flask ml vacuum pump analyt balanc procedur dry ml sinter glass crucibl oven ºc transfer crucibl desicc cool weigh mix sampl lecithin warm water bath weigh nearest mg sampl conic flask add ml toluen swirl mixtur lecithin appar dissolv filter solut sinter glass crucibl wash conic flask ml toluen pass wash crucibl repeat process ml toluen remov excess toluen crucibl suction dry crucibl dry oven ºc hour place desicc cool weigh crucibl residu cool repeat differ weight success weigh mg increas mass occur lowest record read calcul express result formula method calcul content toluen insolubl substanc gt pic file quot quot gt mass gram empti crucibl mass gram crucibl residu initi mass gram sampl repeat differ result determin carri simultan rapid success sampl analyst condit exce mg sampl method limit test reduc substanc sodium potassium calcium lactat scope field applic test detect qualit reduc substanc sodium lactat potassium lactat calcium lactat definit detect limit test concentr reduc substanc limit test result determin method principl fehl solut reduc substanc capabl exhibit reduc action substanc reduc sugar reagent fehl solut copper sulphat pentahydr dissolv water made ml water fehl solut potassium sodium tartrat sodium hydroxid dissolv water made ml water procedur weigh nearest mg sampl dissolv ml warm water add ml fehl solut ml fehl solut boil mixtur minut observ colour chang occur precipit calcium sulphat occur interfer express result limit test interpret colour chang boil test posit presenc reduc substanc sensit limit detect reduc substanc react mg glucos sampl observ result limit test carri simultan rapid success sampl analyst condit ident fehl solut react glucos present sampl method determin volatil acid orthophosphor acid scope field applic method determin volatil acid express acet acid orthophosphor acid definit volatil acid content content volatil acid express acet acid determin method principl water ad sampl solut distil distil titrat standard sodium hydroxid solut acid calcul express acet acid reagent pheolphthalein solut ethanol sodium hydroxid mol apparatu distil apparatu includ sprai trap procedur weigh nearest mg sampl place weigh sampl ml freshli boil cool water distil flask fit sprai trap mix distil ml titrat distil standard mol sodium hydroxid phenolphthalein indic continu titrat red tint solut persist second express result formula method calcul content volatil acid express milligram kilogram acet acid gt pic file quot quot gt volum millilitr mol sodium hydroxid solut neutral mass gram orthophosphor acid sampl repeat differ result determin carri simultan rapid success sampl analyst condit exce mg sampl method limit test nitrat orthophosphor acid scope field applic method detect nitrat orthophosphor acid definit detect limit test concentr nitrat express sodium nitrat limit test result determin method principl sampl ad indigo carmin solut concentr sulphur acid medium blue colour present discharg oxid agent includ nitrat reagent gt pic file quot quot gt procedur measur ml sampl dilut ml sodium chlorid solut add ml carmin indigo solut slowli add ml concentr sulphur acid cool addit note blue colour solut persist minut express result limit test interpret blue colour discharg minut test posit content oxid agent express sodium nitrat greater mg kg observ carri blank test result limit test carri simultan rapid success sampl analyst condit ident indigo carmin solut prepar dai posit result obtain sampl nitrat oxid agent test repeat iso method quot phosphor acid industri includ foodstuff determin oxid nitrogen content xylenol spectrophometr method quot method determin water insolubl substanc present mono di tri sodium orthophosph mono di tri potassium orthophosph ii iii ii iii scope field applic method determin water insolubl matter mono sodium orthophosph di sodium orthophosph ii tri sodium orthophosph iii mono potassium orthophosph di potassium orthophosph ii tri potassium orthophosph iii definit water insolubl matter content water insolubl matter determin method principl sampl dissolv water filter suitabl porcelain crucibl wash dry residu weigh calcul water insolubl matter apparatu sinter porcelain crucibl poros equival desicc freshli activ silica gel water content indic equival desicc oven thermostat control ºc polypropylen beaker ml water bath boil procedur weigh nearest mg sampl phosphat dissolv ml hot water bring boil polypropylen beaker maintain hot water bath minut filter solut previous clean dri weigh crucibl wash insolubl residu hot water place crucibl residu oven dry ºc hour place crucibl desicc cool weigh crucibl repeat dry cool weigh differ weight success weigh mg increas mass occur lowest record read calcul express result formula method calcul content water insolubl matter sampl gt pic file quot quot gt mass gram residu dry mass gram sampl repeat differ result determin carri simultan rapid success sampl analyst condit exce mg sampl method determin ph food addit scope field applic method outlin gener instruct determin ph food addit definit ph food addit ph determin method principl ph aqueou solut dissolv slurri sampl convention determin glass electrod refer electrod ph meter reagent calibr instrument buffer solut buffer solut ph ºc consist equal volum mol potassium dihydrogen phosphat kh po mol disodium hydrogen ortho phosphat dyhidr na hpo buffer solut ph ºc consist mol potassium hydrogen phthalat ko buffer solut ph ºc consist mol sodium borat na satur mol potassium chlorid solut suitabl solut prescrib electrod manufactur fill refer electrod distil water carbon dioxid free ph apparatu ph meter accuraci ph unit electrod combin glass electrod singl glass refer electrod suitabl clamp hold electrod magnet stirrer heater element thermomet calibr rang ºc procedur standard ph meter glass electrod set instruct manufactur ph read electrod regularli check comparison buffer solut ph electrod wash water gentli wipe soft tissu rins water sampl standard solut sampl standard solut sampl consid acid ph buffer solut check ph read ph ph sampl analyz alkalin ph buffer solut check ph read ph ph measur sampl solut concentr sampl sampl prepar procedur adopt prescrib commun food addit direct prepar sampl solut direct distil water adjust ºc stir stop stir place glass electrod solut minut note ph ph meter express result repeat differ result determin carri simultan rapid success sampl analyst condit exce ph unit note method applic ph requir commun food addit direct food addit dissolv slurri water