commiss regul ec amend regul eec determin commun method analysi wine commiss european commun regard treati establish european commun regard council regul eec march common organ market wine amend regul ec articl thereof annex commiss regul eec amend regul ec descript analysi method method analys isotop ratio gt num gt gt den gt oxygen water wine develop valid establish accord internation recogn criteria applic method constitut check authent wine wine product descript method adopt intern wine wine offic method ad regul measur provid regul accord opinion manag committe wine adopt regul articl chapter shown annex hereto ad annex regul eec articl regul enter forc seventh dai public offici journal european commun regul bind entireti directli applic member state brussel commiss franz fischler member commiss oj oj oj oj annex determin isotop ratio gt num gt gt den gt water content wine descript method method object object present method measur isotop ratio gt num gt gt den gt water origin isotop ratio gt num gt gt den gt express deviat amp permil ratio isotop ratio intern refer smow äi amp permil gt num gt ri gt den gt rsmow principl isotop ratio gt num gt gt den gt determin mass spectrometri isotop ratio msir ionic current produc carbon dioxid obtain exchang water wine reaction carbon dioxid gaseou phase analysi reagent carbon dioxid analysi smow standard ocean water gisp greenland ic sheet precipit slap standard light arctic precipit refer water specif laboratori carefulli standard relat refer sampl intern agenc atom energi vienna iaea laboratori equip mass spectromet isotop ratio intern repeat amp permil tripl collector simultan record ion default doubl collector measur ion thermost system carri equilibr water content wine vacuum pump reach intern pressur pa phial sampl ml volum capillari annex tube interior diamet mm eppendorf pipett plastic throw cone experiment determin manual method oper mode equilibr method introduct sampl eppendorf pipett fix volum ml adapt cone pump liquid analys order insert balloon flask place silicon greas neck balloon flask attach balloon flask valv verifi tightli shut repeat oper balloon flask work ramp introduc laboratori refer water balloon degas ramp ramp cool liquid nitrogen system purg mm hg open valv shut valv heat degas cycl repeat pressur variat equilibr water cool work ramp liquid nitrogen alcohol mix freez water put vacuum stabil vacuum isol ramp action valv purg introduct system insert gaseou work ramp isol rest system introduc ramp thermost bath hour night optim time equilibr advis prepar sampl end dai balanc settl overnight transfer exchang measur cell sampl holder support measur cell balloon flask exchang adapt empti line work ramp empti cell carefulli purg exchang gase contain ballon transfer measur cell cool liquid nitrogen measur cell allow heat room temperatur automat exchang apparatu order carri equilibr sampl phial fill ml wine ml water laboratori work refer cool sampl slide frozen product adapt equilibr system creat vacuum system carbon dioxid introduc pressur hpa balanc reach temperatur minimum period hour moder agit equilibr durat depend phial geometri optimum durat determin system carbon dioxid contain phial transfer introduct chamber mass spectromet capillari tube measur carri specif protocol kind equip calcul express result rel differ ratio intens ion gt num gt gt den gt sampl refer express amp permil mean equat sampl gt num gt gt num gt gt den gt sampl gt den gt gt num gt gt den gt refer content sampl compar refer smow smow slap scale relat gt num gt sampl smow gt den gt smow slap accept slap equal amp permil compar smow isotop ratio refer determin seri measur unknown sampl fidel repeat equal amp permil reproduct equal amp permil