commiss regul ec collect sampl adopt detail rule connect physic check boneless beef cut qualifi export refund commiss european commun regard treati establish european commun regard council regul ec common organis market beef veal amend commiss regul ec articl thereof regard council regul eec februari monitor carri time export agricultur product receiv refund amount amend regul ec articl thereof accord articl regul ec differ price product list articl regul world market commun cover export refund agricultur product arrang govern system set commiss regul ec april lai common detail rule applic system export refund agricultur product amend regul ec sector annex commiss regul eec decemb establish agricultur product nomenclatur export refund amend regul ec inter alia refund grant boneless cut condit minimum lean bovin meat content case cut adult male bovin anim individu wrap commiss regul ec amend regul ec lai detail rule physic check carri time export agricultur product qualifi refund special condit beef laid commiss regul ec decemb collect sampl connect physic check boneless beef cut qualifi export refund provis made check proven origin boneless cut adult male bovin anim methodolog appli check view standardis set penalti event failur compli condit proven origin regul ec addit updat order account nomenclatur applic export refund agricultur product provid regul eec amend regul ec interest clariti regul ec repeal replac measur provid regul accord opinion manag committe beef veal adopt regul articl regul appli event physic check natur characterist product mean articl regul eec complianc oblig individu wrap boneless cut cover product code origin boneless cut adult male bovin anim cover product code minimum averag lean meat content boneless cut cover product code descript product refer paragraph contain nomenclatur agricultur product export refund set sector annex regul eec articl purpos physic check sampl consist full carton collect part consign carton intend author respons check reserv supervis custom author quantiti product cover declar deem compris consign declar refer articl regul ec declar refer articl regul ec situat refer paragraph storag articl purpos check complianc oblig refer articl custom author check cut carton sampl refer articl individu wrap packag cut case check conduct carton take carton cut turn wrap individu packag cut condit refund met consign deem irregular condit observ irregular deem occur irregular establish refund payabl respect consign calcul basi correct weight correct weight obtain reduc declar net weight percentag weight cut fail compli relat total net weight sampl articl purpos check condit origin refer articl sampl analysi consist cut collect random carton sampl refer articl analysi show meat beef adult male bovin anim present refund grant check carri accord methodolog annex hereto prejudic check decid irregular suspect check carri random cover export oper oper select physic check articl purpos check complianc condit refer articl content carton sampl refer articl minc form homogen mixtur lean meat content sampl minimum laid content carton examin averag carton averag lean meat content laid refund grant articl accord articl prejudic articl council regul eec sampl collect check provid regul time declar refer articl accept verifi articl regul ec repeal articl regul enter forc juli appli oper cover declar refer articl cover effect juli regul bind entireti directli applic member state brussel commiss franz fischler member commiss oj oj oj oj oj oj oj oj oj oj oj oj oj annex analysi origin boneless beef cut adult male bovin anim methodolog appli beef sex methodolog appli base polymeras chain reaction pcr compris sampl extract dna pcr gel electrophoresi sampl sampl sampl dissect core part insid provid meat piec steril knife scalpel similar sampl ground micro grinder cut smaller piec assur reason extract effici sampl prepar work place perform pcr handl materi perform environ clean easili prefer clean bench ensur cross contamin sampl occur steril blade scalpel similar tool prepar meat sampl extract purif dna dna extract purif accomplish convent procedur readi kit base principl solubilis meat sampl lysi buffer surfact deterg proteinas applic solubilis sampl dna bind resin remov dna compound repeat wash step final elut purifi dna water low salt buffer extract dna sodium hydroxid solut control success extract gel electrophoresi recommend mandatori valid aspect batch sampl extract extract control meat perform parallel prove integr appli procedur polymeras chain reaction pcr principl principl pcr step procedur denatur anneal primer extens repeat time number quot cycl quot method reagent reaction buffer mgcl deoxynucleotid primer heat stabl dna polymeras steril water mix develop method lead quot mastermix quot dedic pipett prepar mastermix mastermix ad dna templat extract dna reaction perform thermo cycler complet pcr product analys gel electrophoresi store recommend method appli refer templat amplifi sequenc amelogenin locu homolog gene zfx region allel specif pcr specif primer type method amelogenin forward cagccaaacctccctctgc amelogenin revers cccgcttggtcttgtctgttgc enni gallagh anim genet amelogenin forward aaattctctcacagtccaag amelogenin revers caacaggtaattttcctttag chen hu wang hung wu choo cheng mol reprod dev zfx allel specif forward gacagctgaacaagtgttactg zfx allel specif revers aatgtcacacttgaatcgcatc zfy allel specif forward gaaggccttcgaatgtgataac zfy allel specif revers ctgacaaaaggtggcgatttca kirkpatrick monson reprod fertil zfx forward agctgaacaagggttactg zfy forward caagcttaccagcaagtca zfx revers ccagtatggattcgcatgt zinovieva palma müller brem theriogenolog pcr mastermix prepar clean bench decontamin work deterg uv light method develop alter publish method requir exact mastermix composit mgcl concentr primer concentr amount templat dna adapt temperatur program temperatur hold time appear specif amplif product lead optimis anneal temperatur mgcl concentr primer concentr guarante accuraci result valid aspect method appli routin analysi properli valid analysi control includ set sampl extract control meat neg pcr control refer sampl male femal beef sampl meat beef revalid appli kei compon procedur chang dna polymeras supplier product primer lot good laboratori practic deem indispens proper clean decontamin work place instrument aliquot primer dedic pipett analysi amplicon gel electrophoresi pcr fragment amplicon obtain analys gel electrophoresi agaros gel stain ethidium bromid polyacrylamid gel silver stain complet electrophoret separ applic molecular weight marker gel determin approxim size obtain amplicon document obtain result properli document gel imag descript result note expect result contamin dna contamin dna sambrook fritsch maniati ed molecular clone cold spring harbour laboratori press elphineston hennessei wilson stead bulletin oepp eppo pcr method recommend subject approv offici design refer laboratori