commiss decis march lai detail rule implement council direct eec maximum level method analysi marin biotoxin bivalv mollusc echinoderm tunic marin gastropod notifi document number text eea relev ec commiss european commun regard treati establish european commun regard council direct eec juli lai health condit product place market live bivalv mollusc amend direct ec chapter paragraph annex thereto chapter point annex direct eec customari biolog test method give posit result presenc diarrhet shellfish poison dsp edibl part mollusc bodi part edibl separ scientif proven marin biotoxin diarrhet shellfish poison dsp complex okada acid oa dinophysistoxin dtx yessotoxin ytx pectenotoxin ptx azaspiracid aza pose hazard human health present limit bivalv mollusc echinoderm tunic marin gastropod light recent scientif studi establish maximum level method analysi biotoxin maximum level method analysi harmonis implement member state order protect human health addit biolog test method altern detect method chemic method vitro assai accept demonstr perform chosen method effect perform biolog method implement equival level public health protect propos maximum level base provision data evalu scientif evid measur provid decis accord opinion stand veterinari committe adopt decis articl decis lai maximum level marin biotoxin diarrhet shellfish poison dsp complex okada acid dinophysistoxin yessotoxin pectenotoxin azaspiracid method analysi detect appli bivalv mollusc echinoderm tunic marin gastropod intend human consumpt process consumpt articl maximum level okada acid dinophysistoxin pectenotoxin anim refer articl bodi part edibl separ μg okada acid equival kg method analysi set annex articl maximum level yessotoxin anim refer articl bodi part edibl separ mg yessotoxin equival kg method analysi set annex articl maximum level azaspiracid anim refer articl bodi part edibl separ μg azaspiracid equival kg method analysi set annex articl result analys perform demonstr discrep method mous bioassai consid refer method articl decis address member state brussel march commiss david byrn member commiss oj oj annex detect method biolog method seri mous bioassai procedur differ test portion hepatopancrea bodi solvent extract purif step detect toxin mention articl sensit select depend choic solvent extract purif step account make decis method order cover full rang toxin singl mous bioassai involv aceton extract detect okada acid dinophysistoxin pectenotoxin yessotoxin assai complement liquid liquid partit step ethyl acet water dichloromethan water remov potenti interfer azaspiracid detect regulatori level mean procedur requir bodi test portion mice test death mice hour inocul extract equival hepatopancrea bodi consid posit result presenc toxin mention articl level establish articl mous bioassai aceton extract liquid liquid partit diethyleth detect okada acid dinophysistoxin pectenotoxin azaspiracid detect yessotoxin loss toxin place partit step mice test death mice hour inocul extract equival hepatopancrea bodi consid posit result presenc okada acid dinophysistoxin pectenotoxin azaspiracid level establish articl rat bioassai detect okada acid dinophysistoxin azaspiracid rat test diarrhet respons rat consid posit result presenc okada acid dinophysistoxin azaspiracid level mention articl altern detect method seri method high perform liquid chromatographi hplc fluorimetr detect liquid chromatographi lc mass spectrometri ms inmunoassai function assai phosphatas inhibit assai altern complementari method biolog test method provid combin detect analogu effect biolog method implement equival level public health protect okada acid dinophysistoxin hydrolysi step requir order detect presenc dtx pectenotoxin ptx ptx yessotoxin ytx ytx homo ytx homo ytx azaspiracid aza aza aza analogu public health signific discov includ analysi standard chemic analysi total toxic calcul convers factor base toxic data toxin perform characterist method defin valid internation agre protocol