commiss decis januari commun health condit import anim fresh meat includ minc meat countri amend decis eec ec ec notifi document number text eea relev ec commiss european commun regard treati establish european commun regard council direct eec decemb health veterinari inspect problem import bovin ovin caprin anim swine fresh meat meat product countri amend regul ec articl thereof regard council direct eec juli lai principl govern organis veterinari check anim enter commun countri amend direct eec eec eec amend direct ec articl thereof regard council direct eec june public health anim health problem relat kill wild game place market wild game meat amend regul ec articl thereof regard council direct eec juli lai anim health requir govern trade import commun anim semen ova embryo subject anim health requir laid specif commun rule refer annex direct eec amend commiss regul ec articl thereof regard council direct eec decemb lai public health anim health requir govern trade import commun product subject requir laid specif commun rule refer annex chapter direct eec pathogen direct eec amend commiss decis ec articl thereof regard council direct eec decemb lai requir product place market minc meat meat prepar amend regul ec articl thereof regard regul ec european parliament council lai rule prevent control erad transmiss spongiform encephalopathi amend commiss regul ec articl thereof regard council direct ec decemb lai anim health rule govern product process distribut introduct product anim origin human consumpt articl thereof commun provis anim health condit import countri live anim fresh meat meat product requir gener term import authoris countri part thereof appear list authoris countri ii anim product fulfil health condit certifi offici veterinarian export countri mean certif view simplifi legisl commun legisl occur anim diseas situat countri group singl legal act import condit includ list countri model certif applic import commun kind artiodactyla proboscidea fresh meat anim equida council decis eec draw list countri member state authoris import bovin anim swine equida sheep goat fresh meat meat product provis articl direct eec decis histor main refer initi commun procedur export countri larg varieti anim deriv superced fresh meat includ minc meat council direct ec updat decis eec basi present consolid articl council direct eec june anim health condit govern movement import countri equida establish import anim allow countri part thereof appear list incorpor list countri laid provis articl direct eec commiss decis adopt basi direct eec health condit import equida provid list countri authoris export anim commun rule govern anim health condit import live anim pursuant direct eec provis articl refer list countri authoris export live anim replac council direct lai anim health rule import commun live anim amend direct eec eec eec ec context articl direct eec amend order establish principl draw specif list countri authoris specif model health certif requir export equida commun direct forese specif provis anim import commun commerci transact reason specif trade anim import commerci transact exclud equida anim show exhibit scientif includ conserv experiment purpos circus scope decis eec import meat product basi provis direct eec eec eec commiss decis ec ec lai anim health condit model veterinari certif list countri member state authoris import meat product obtain larg varieti anim decis provid harmonis rule anim health condit import countri meat product order avoid duplic list countri authoris export product exclud meat product scope decis eec establish live anim regularli bred direct eec definit quot hold quot domest bovin ovin caprin porcin anim direct eec defin quot approv bodi institut centr quot kind anim view harmonis simplifi terminolog purpos decis consolid singl definit establish kind artiodactyla proboscidea regularli bred commiss decis ec lai anim public health condit veterinari certif import minc meat meat prepar countri deep frozen minc meat made meat domest bovin ovin caprin porcin anim concern anim diseas anim health appli fresh meat anim meat prepar incorpor meat mammallian anim decis ec amend order remov scope minc meat domest cloven hoof anim incorpor harmonis manner decis eec maintain condit applic import meat prepar separ decis commiss decis ec lai anim public health condit veterinari certif import wild farm game meat rabbit meat countri list meat farm wild cloven hoof game equida concern anim diseas anim health appli fresh meat domest bovin swine equida sheep goat decis ec amend order withdraw scope import condit meat farm wild cloven hoof game equida incorpor harmonis manner decis eec requir direct ec commiss decis eec adopt basi direct eec condit approv border inspect post import live bovin anim swine fresh meat deriv therefrom entri forc council direct eec eec implement commiss decis provis decis eec longer applic repeal commiss decis eec establish protocol standardis materi procedur veterinari diagnost test condit approv market connect import domest anim bovin porcin speci countri provis approv market trade anim intend export european commun longer applic decis repeal provis protocol standardis materi procedur veterinari test incorpor harmonis manner decis eec commiss decis ec ec lai anim health condit veterinari certif import sheep goat list countri member state authoris import anim commiss decis ec establish anim health condit veterinari certif import live bovin anim swine list countri member state authoris import cloven hoof anim cover direct eec health condit veterinari certif list countri authoris import anim commun harmonis commun level anim concern anim diseas anim health appli decis ec ec ec repeal provis incorpor harmonis manner decis eec order preserv health statu anim export countri transport commun specif provis establish commiss decis eec record complianc member state region requir relat brucellosi melitensi accord statu member state region offici free diseas enabl member state requir addit guarante deliv certif commiss decis eec eec eec eec eec eec eec eec eec eec eec eec eec eec eec eec eec eec eec eec eec ec ec ec ec ec provid anim health condit veterinari certif import fresh meat domest bovin ovin caprin porcin equin anim countri decis repeal provis incorpor harmonis manner decis eec intern offic epizoot diseas codex alimentariu set guidelin principl certif veterinarian follow prescrib principl certifi veterinarian certifi matter knowledg time sign certif separ attest offici compet author council direct ec lai standard certif valid certif prevent fraud ensur rule principl appli countri certifi offic provid guarante equival laid direct ec model veterinari certif laid decis eec exclus reflect fact attest time issu certif view inform certifi veterinarian import compet author member state certif present detail establish note refer period valid certif date issu coverag reason explan definit supplementari guarante specif condit health requir hold premis anim accompani model certif unit nation establish guidelin common frame layout draw commerci document aegi intern bodi deal facilit procedur intern trade principl rule issu certif intend intern transact certif procedur intern offic epizoot diseas codex alimentariu set guidelin electron certif view harmonis layout veterinari certif issu deliv offici veterinarian export countri facilit electron mean transfer certif model veterinari certif laid decis eec format note produc certif export countri annex direct eec lai specimen public health certif import countri fresh meat domest bovin ovin caprin porcin equin anim intend human consumpt meat farm wild game anim health public health attest requir includ anim welfar incorpor model certif categori meat commiss decis ec commun measur adopt area anim welfar public health concern import live anim fresh meat view harmonis import condit ensur transpar commun requir simplifi legisl procedur updat commun legisl incorpor model veterinari certif laid decis eec relev requir export commun categori anim meat health provis gener scope adopt commun level condit ultim accept import commun commod provis laid council direct ec april measur monitor substanc residu thereof live anim anim product regul ec european parliament council januari lai gener principl requir food law establish european food safeti author lai procedur matter food safeti result safeguard measur provid direct eec ec tariff statist nomenclatur worldwid classifi live anim meat deriv categori alloc specif code definit declar custom author commod import account categori anim product provis direct eec ec veterinari check commun frontier requir close collabor border inspect post custom offic anim product anim origin arriv commun view harmonis facilit import procedur commun frontier consign present import accompani veterinari certif addit australia fresh poultrymeat live poultri ratit meat ratit import specif test requir due newcastl diseas vaccin compli commun legisl import wild farm game meat suspend situat asseses categori bird decis eec decis ec amend commun provis import product anim origin make refer list countri appear annex decis eec amend decis eec refer constru list countri part thereof appear part annex ii decis eec amend present decis base direct eec commiss decis eec eec eec harmonis anim health condit veterinari certif rule govern import countri raw materi process industri fresh meat domest bovin ovin caprin porcin equin anim intend human consumpt addit commiss decis list recit repeal present act provid anim health rule certif import fresh meat intend pet food process industri regul ec european parliament council lai health rule anim product intend human consumpt includ raw materi certif requir review implement regul ec fulli applic meantim maintain date anim health requir model certif decis requir product provid transit period continu accept model certif measur provid decis accord opinion stand committe food chain anim health adopt decis articl decis eec amend titl replac quot council decis eec decemb draw list countri part countri lai anim public health veterinari certif condit import commun live anim fresh meat quot articl replac quot articl subject matter scope decis establish sanitari condit import commun live anim exclud equida import fresh meat meat product anim includ equida exclud meat prepar decis appli import domest anim show exhibit anim regularli bred domest anim form part circus intend scientif includ conserv experiment purpos bodi institut centr approv accord annex direct eec import anim fresh meat authoris accord decis remain subject provis adopt adopt european food law articl definit purpos decis definit appli anim mean land mammal speci belong taxa proboscidea artiodactyla crossbre hold mean farm offici supervis agricultur industri commerci undertak includ zoo amus park wildlif hunt reserv anim regularli bred trim offal mean offal bone cartilag trachea main bronchi lymphat gland adher connect tissu fat mucu complet remov case meat domest bovin anim masset muscl incis accord point chapter viii annex council direct eec consid trim offal articl condit import live anim commun import commun live anim allow anim compli articl articl place origin live anim anim territori countri part thereof list column tabl set part annex column specif model veterinari certif design anim articl specif condit anim meet requir set certif establish accord model certif drawn part annex take account specif condit column tabl set part annex column tabl meet supplementari guarante requir certif requir member state destin anim concern meet addit certif requir mention member state includ certif base model set part articl transport live anim import commun anim load mean transport carri anim destin commun lower health statu transport commun anim unload territori countri part countri approv import commun anim transport commun anim move road railwai foot territori part territori countri approv import commun anim anim arriv border inspect post commun dai date load export countri accompani veterinari certif drawn conform model complet sign offici veterinarian export countri case transport sea period dai prolong time sea journei purpos declar master ship drawn accord addendum part annex attach origin form veterinari certif articl condit appli import import accord direct eec anim intend slaughter convei delai slaughterhous destin slaughter work dai ii anim intend breed product fatten purpos anim intend zoo amus park hunt wildlif reserv convei delai hold destin remain minimum period dai movement hold case direct dispatch slaughterhous articl condit import fresh meat commun import commun fresh meat intend human consumpt anim defin articl equida allow meat compli articl articl place origin fresh meat fresh meat territori countri part thereof list column tabl set part annex ii column specif model veterinari certif design meat articl specif condit fresh meat meet requir set certif model certif drawn part annex ii take account specif condit column tabl set part annex ii column tabl meet supplementari guarante request certif articl present fresh meat commun border inspect post fresh meat present commun border inspect post accompani veterinari certif drawn conform model complet sign offici veterinarian export countri articl condit appli import import categori fresh meat convei delai process establish destin accord direct ec unskin carcas wild cloven hoof game intend human consumpt process trim offal domest bovin anim intend human consumpt meat base product heat treatment cook core temperatur sterilis hermet seal contain achiev fo amp gt categori product refer paragraph establish destin establish specif approv regist process product member state establish situat accord procedur establish decis ec member state commun commiss name address establish refer paragraph local compet author respons supervis establish categori product establish approv regist articl certif veterinari certif requir import live anim fresh meat commun provid decis draft accord note set part annex ii preclud electron certif agre system harmonis commun level quot articl articl annex replac annex decis articl amend decis ec decis ec amend titl replac quot commiss decis ec septemb lai anim public health veterinari certif condit import meat prepar commun countri quot articl replac quot articl decis lai anim public health veterinari certif condit import meat prepar quot articl delet articl delet articl delet annex delet articl amend decis ec decis ec amend titl replac quot commiss decis ec septemb draw list countri member state authoris import rabbit meat wild farm game meat lai anim public health veterinari certif condit import quot articl replac quot articl member state authoris import meat meat wild game bird offal case unpluck uneviscer game bird meat farm game bird meat wild leporida defin rabbit hare offal case unskin uneviscer leporida meat farm rabbit meat wild land mammal ungul leporida offal meat countri part countri list annex compli condit laid veterinari certif establish accord relev model health certif set annex iii provid annex ii specif requir refer annex ii set annex iv fulfil export countri certifi complet section health certif accord model set annex iii quot annex ii replac annex decis annex iii model delet annex iv paragraph delet articl repeal decis eec eec eec eec eec eec eec eec eec eec eec eec eec eec eec eec eec eec eec eec eec eec eec eec eec ec ec ec ec ec ec repeal articl transit final provis decis appli refer made commun legisl list countri appear part annex decis eec laid amend introduc present decis constru refer list countri appear part annex ii decis eec amend present decis import commun live anim certifi date refer paragraph fresh meat anim slaughter date refer paragraph accord model veterinari certif laid decis refer articl authoris period dai applic decis articl decis address member state brussel januari commiss david byrn member commiss oj oj oj oj oj oj oj oj oj oj oj oj oj oj oj oj final oj oj oj oj oj oj oj oj oj oj oj oj oj oj oj oj oj oj oj oj oj oj oj oj oj oj oj oj oj oj oj oj oj oj oj oj oj oj oj oj oj oj oj oj annex quot annex live anim part list countri part thereof gt tabl gt specif condit footnot certif quot quot territori presenc bse nativ cattl assess highli purpos export european commun anim certifi model certif bov bov quot ii quot territori recognis offici tuberculosi free statu purpos export european commun anim certifi model certif bov quot iii quot territori recognis offici brucellosi free statu purpos export european commun anim certifi model certif bov quot iva quot territori recognis offici enzoot bovin leukosi ebl free statu purpos export european commun anim certifi model certif bov quot ivb quot territori approv hold recognis offici enzoot bovin leukosi ebl free statu purpos export european commun anim certifi model certif bov quot quot territori recognis offici brucellosi free statu purpos export european commun anim certifi model certif ovi quot vi quot geograph constraint case bulgaria code territori bg anim certifi model veterinari certif bov bov rum ovi ovi import part territori member state appear annex ii decis ec februari member state quot vii quot territori recognis offici tuberculosi free statu purpos export european commun anim certifi model certif rum quot viii quot territori recognis offici brucellosi free statu purpos export european commun anim certifi model certif rum quot ix quot territori recognis offici aujeszki diseas free statu purpos export european commun anim certifi model certif por part model veterinari certif model quot bov quot model veterinari certif domest bovin anim bo tauru bison bison bubalu bubali cross breed intend breed product import quot bov quot model veterinari certif domest bovin anim bo tauru bison bison bubalu bubali cross breed intend slaughter import quot ovi quot model veterinari certif domest sheep ovi ari goat capra hircu intend breed product import quot ovi quot model veterinari certif domest sheep ovi ari goat capra hircu intend slaughter import quot por quot model veterinari certif domest porcin anim su scrofa intend breed product import quot por quot model veterinari certif domest porcin anim su scrofa intend slaughter import quot rum quot model veterinari certif domest anim suida quot sui quot model veterinari certif domest suida sg supplementari guarante quot quot guarante bluetongu epizoot haemorrhag diseas test anim certifi model certif bov point ovi point rum point quot quot guarante swine vesicular diseas classic swine fever test anim certifi model certif por point sui point quot quot guarante brucellosi test anim certifi model certif por point sui point note veterinari certif produc export countri base model appear part annex layout model correspond anim concern number order appear model attest requir countri case supplementari guarante requir export countri part thereof request eu member state destin anim concern addit certif requir incorpor origin form veterinari certif separ uniqu certif provid anim export singl territori appear column part annex consign destin transport railwai wagon lorri aircraft ship origin certif consist singl page side text requir form page need part integr indivis drawn offici languag eu member state inspect border post carri eu member state destin member state commun languag accompani offici translat reason identif item consign schedul point model certif addit page attach certif page consid form part origin certif applic signatur stamp certifi offici veterinarian page certif includ addit schedul refer compris page page number page number total number page bottom bear code number certif design compet author top origin certif complet sign offici veterinarian hour prior load consign export commun compet author export countri ensur principl certif equival laid council direct ec colour signatur print rule appli stamp emboss watermark origin certif accompani consign reach eu border inspect post certif valid dai date issu case transport ship time valid prolong time trip ship purpos declar master ship drawn accord addendum part annex decis attach origin form veterinari certif anim transport anim destin european commun lower health statu transport european commun anim unload territori countri part countri approv import commun anim gt pic file quot en tif quot gt gt pic file quot en tif quot gt gt pic file quot en tif quot gt gt pic file quot en tif quot gt gt pic file quot en tif quot gt gt pic file quot en tif quot gt gt pic file quot en tif quot gt gt pic file quot en tif quot gt gt pic file quot en tif quot gt gt pic file quot en tif quot gt gt pic file quot en tif quot gt gt pic file quot en tif quot gt gt pic file quot en tif quot gt gt pic file quot en tif quot gt gt pic file quot en tif quot gt gt pic file quot en tif quot gt gt pic file quot en tif quot gt gt pic file quot en tif quot gt gt pic file quot en tif quot gt gt pic file quot en tif quot gt gt pic file quot en tif quot gt gt pic file quot en tif quot gt gt pic file quot en tif quot gt gt pic file quot en tif quot gt gt pic file quot en tif quot gt gt pic file quot en tif quot gt gt pic file quot en tif quot gt gt pic file quot en tif quot gt part addendum transport anim sea complet attach veterinari certif transport european commun frontier includ part journei transport ship gt pic file quot en tif quot gt condit authoris assembl centr approv assembl centr meet requir supervis offici veterinarian ii situat centr area km diamet offici find dai prior approv centr case foot mouth diseas iii approv centr clean disinfect disinfect offici authoris export countri effect control diseas mention condit ii iv take account anim capac facil dedic exclus purpos facil easi clean disinfect load unload adequ hous suitabl standard anim water feed give treatment facil inspect isol equip clean disinfect room truck storag area fodder litter manur system collect dispos wast water offic offici veterinarian oper suffici veterinarian carri duti vi admit anim individu identifi guarante traceabl end anim admit owner person charg centr ensur anim properli identifi accompani health document certif speci categori involv person record regist databas retain year owner origin date entri exit number identif anim registr number herd origin destin registr number carrier registr number lorri deliv collect anim premis vii anim pass fulfil health condit establish import relev categori anim european commun viii anim export european commun pass assembl centr dai arriv load dispatch directli frontier export countri come contact cloven hoof anim anim fulfil health condit establish import relev categori anim european commun segreg consign consign anim breed product anim slaughter transport vehicl contain clean disinfect disinfect offici authoris export countri effect control diseas mention condit ii construct faec urin litter fodder flow fall transport ix condit export anim commun requir test carri period load period includ period assembl dai arriv anim approv centr export countri design approv centr approv anim breed product approv centr approv anim slaughter notifi commiss compet central author member state name address premis regular updat xi export countri determin procedur offici supervis approv centr ensur supervis carri xii regularli inspect order ascertain requir approv continu fulfil case failur suspens approv restor compet author satisfi full complianc centr provis mention protocol standardis materi test procedur tuberculosi tbl singl intraderm tuberculin test bovin tuberculin carri annex direct eec case suida anim singl intraderm tuberculin test avian tuberculin carri annex eec site inject loos skin base ear brucellosi brucella abortu brl serum agglutin test complement fixat test buffer brucella antigen test enzym link immuno absorb assai test elisa carri annex direct eec brucellosi brucella melitensi brl test carri annex direct eec enzoot bovin leukosi ebl agar gel immuno diffus test enzym link immuno absorb assai test elisa carri paragraph chapter ii annex council direct eec bluetongu btg block competit elisa test carri protocol competit elisa monoclon antibodi capabl identifi antibodi serotyp bluetongu viru btv principl test interrupt reaction btv antigen group specif monoclon antibodi addit test serum antibodi btv present test serum block reactiv monoclon antibodi mab result reduct expect colour develop addit enzym label anti mous antibodi chromogen substrat sera test singl dilut spot test appendix titrat serum titrat appendix give dilut end point inhibit valu higher regard posit materi reagent elisa microtitr plate antigen suppli cell extract concentr prepar store block buffer phosphat buffer salin pb btv neg adult bovin serum tween suppli polyoxyethylen sorbiton monolaur syrup pb monoclon antibodi suppli hybridoma tissu cultur supernat direct group specif polypeptid vp store freez dri dilut block buffer conjug rabbit anti mous globulin adsorb elut conjug horseradish peroxidas dark chromogen substrat orthophenylen diamin opd chromogen final concentr mg ml steril distil water hydrogen peroxid substrat ad immedi µl ml opd handl opd care wear rubber glove suspect mutagen molar sulphur acid ml acid ad ml distil water rememb add acid water water acid orbit shaker elisa plate reader test read visual test format cc conjug control serum monoclon antibodi strong posit serum control weak posit serum control neg serum control cm monoclon antibodi control serum appendix spot dilut format sera plate gt tabl gt appendix serum titrat format sera plate gt tabl gt test protocol conjug control cc well blank control consist btv antigen conjug blank elisa reader mab control cm column row monoclon antibodi control btv antigen monoclon antibodi conjug well repres maximum colour optic densiti read control repres inhibit posit control column row well btv antigen btv strong weak posit antiserum mab conjug neg control well neg control btv antigen btv neg antiserum mab conjug test sera larg scale serolog survei rapid screen sera test singl dilut appendix altern sera test dilut rang appendix give indic titr antibodi test sera procedur dilut btv antigen pre titrat concentr pb sonic briefli dispers aggreg viru sonic pipett vigor add μl well elisa plate tap side plate dispers antigen incub minut orbit shaker wash plate time flood empti well steril pb blot dry absorb paper control well add μl block buffer cc well add μl posit neg control sera dilut μl sera μl block buffer respect well add μl block buffer mab control well spot titrat method add dilut test serum block buffer duplic well column μl sera μl block buffer serum titrat method prepar fold dilut seri test sampl block buffer well singl column immedi addit test sera dilut mab block buffer add μl well plate blank control incub minut orbit shaker wash time pb blot dry dilut rabbit anti mous concentr block buffer add μl well plate incub minut orbit shaker wash time pb blot dry thaw opd immedi add μl hydrogen peroxid ml opd add μl well plate colour develop approxim minut stop reaction sulphur acid μl colour develop mab control well well sera antibodi btv examin record plate visual spectrophotometr reader analysi result softwar packag print od valu percentag inhibit pi test control sera base record antigen control well date express od pi valu determin test perform accept limit upper control limit ucl lower control limit lcl mab control antigen mab absenc test sera od valu plate fail conform criteria reject comput softwar packag print od valu elisa printer calcul od antigen control well equival determin od manual calcul posit neg sampl percentag inhibit pi od test control od cm duplic neg control serum well duplic blank well record pi valu failur limit invalid plate suggest background colour develop strong weak posit control sera record pi valu diagnost threshold test sera pi od sampl record pi valu amp gt record neg sampl record pi valu threshold duplic well consid doubt sampl retest spot test titrat posit sampl titrat provid indic degre posit visual read posit neg sampl easili discern ey weakli posit strong neg sampl difficult interpret ey prepar btv elisa antigen wash roux confluent bhk cell time serum free eagl medium infect bluetongu viru serotyp serum free eagl medium incub examin daili cytopath effect cpe cpe complet cell sheet roux harvest viru shake attach cell glass centrifug rpm pellet cell discard supernat suspend cell approxim ml pb quot sarkosyl quot ml phenylmethylsulphonyl fluorid lysi buffer cell form gel lysi buffer ad reduc effect nb phenylmethylsulphonyl fluorid harm handl extrem caution disrupt cell second ultrason probe amplitud micron centrifug rpm minut store supernat resuspend remain cell pellet ml lysi buffer sonic clarifi store supernat stage total time pool supernat centrifug rpm minut ml cushion sucros pb ml beckmann centrifug tube sw rotor discard supernat drain tube suspend pellet pb sonic store antigen aliquot titrat btv elisa antigen bluetongu elisa antigen titrat indirect elisa twofold dilut antigen titrat constant dilut monoclon antibodi protocol titrat dilut btv antigen pb microtitr plate twofold dilut seri μl multichannel pipett incub hour orbit shaker wash plate time pb add μl monoclon antibodi dilut microtitr plate incub hour orbit shaker wash plate time pb add μl rabbit anti mous globulin conjug horseradish peroxidas dilut pretitr optim concentr microtitr plate incub hour orbit shaker add substrat chromogen previous stop reaction minut addit molar sulphur acid μl competit assai monoclon antibodi excess dilut antigen chosen fall titrat curv plateau region approxim od minut agar gel immuno diffus test carri protocol antigen precipit antigen prepar cell cultur system support rapid multipl refer strain bluetongu viru bhk vero cell recommend antigen present supernat fluid end viru growth requir fold concentr effect achiev standard protein concentr procedur viru antigen inactiv addit beta propiolacton posit control serum intern refer serum antigen nation standard serum produc standardis optim proport intern refer serum freez dri control serum test test serum procedur agaros prepar borat sodium barbitol buffer ph pour petri dish minimum depth mm test pattern moistur free well mm diamet cut agar pattern consist centr well arrang round circl radiu cm central fill standard antigen peripher well fill posit serum well fill test sera system incub hour room temperatur close humid chamber interpret test serum posit form specif precipitin line antigen form complet line ident control serum test serum neg form specif line antigen bend line control serum petri dish examin dark background indirect illumin epizoot haemorrhag diseas ehd agar gel immuno diffus test carri protocol antigen precipit antigen prepar cell cultur system support rapid multipl serotyp epizoot haemorrhag diseas viru bhk vero cell recommend antigen present supernat fluid end viru growth requir fold concentr effect achiev standard protein concentr procedur viru antigen inactiv addit beta propiolacton posit control serum intern refer serum antigen nation standard serum produc standardis optim proport intern refer serum freez dri control serum test test serum procedur agaros prepar borat sodium barbitol buffer ph pour petri dish minimum depth mm test pattern moistur free well mm diamet cut agar pattern consist centr well arrang round circl radiu cm central fill standard antigen peripher well fill posit serum well fill test sera system incub hour room temperatur close humid chamber interpret test serum posit form specif precipitin line antigen form complet line ident control serum test serum neg form specif line antigen bend line control serum petri dish examin dark background indirect illumin infecti bovin rhinotrach ibr infecti pustular vulvo vagin ipv serum neutralis test carri protocol serum sera heat inactiv minut procedur constant viru vari serum neutralis test microtitr plate emploi mdbk suscept cell colorado oxford refer strain viru tcid ml inactiv undilut serum sampl mix equal volum ml viru suspens viru serum mixtur incub hour microtitr plate mdbk cell ad cell concentr form complet monolay hour control viru infect assai ii serum toxic control iii uninocul cell cultur control iv refer antisera interpret result neutralis test titr viru test record dai incub serum titr consid neg neutralis dilut undilut serum test recognis frame commiss decis ec addit guarante infecti rhinotrach bovin destin member state region thereof free diseas foot mouth diseas fmd collect oesophag pharyng sampl test carri protocol reagent prior sampl transport medium prepar ml volum dispens contain anim sampl contain withstand freez solid liquid nitrogen sampl obtain special design sputum collector quot probang quot obtain sampl probang cup pass mouth dorsum tongu upper part oesophagu attempt made scrape surfac epithelium upper oesophagu pharynx movement direct later dorsal probang withdrawn prefer anim swallow cup full mixtur mucu saliva oesophag fluid cellular debri care ensur specimen visibl cellular materi rough handl bleed avoid sampl anim heavili contamin rumin content sampl discard mouth anim flush water prefer physiolog salin repeat sampl treatment sampl sampl collect probang cup examin qualiti ml ad equal volum transport medium contain withstand freez contain tightli close seal disinfect label sampl cool examin hour dry ic liquid nitrogen frozen examin anim probang disinfect wash clean water test fmd viru sampl inocul cultur primari bovin thyroid cell cultur tube sampl suscept cell primari bovin porcin kidnei cell mind strain fmd viru sensit tube incub roller apparatu examin daili hour presenc cytopath effect cpe neg cultur blind passag cultur examin hour specif cpe confirm recommend transport media phosphat buffer ph bovin serum albumin phenol red antibiot tissu cultur medium eagl mem hepe buffer bovin serum albumin antibiot ph antibiot ml final ad transport medium penicillin iu neomycin sulphat iu polymyxin sulphat iu mycostatin iu viru neutralis test carri protocol reagent stock fmdv antigen prepar cell cultur cattl tongu store addit glycerol stock antigen fmdv stabl condit titr vari period month procedur test carri flat bottom tissu cultur grade microtitr plate suscept cell ib rs bhk calf kidnei cell sera test dilut serum free cell cultur medium addit iu ml neomycin suitabl antibiot sera inactiv minut ml amount prepar twofold seri microtitr plate ml dilut loop pretitr viru dilut serum free cultur medium tcid ml ad incub hour neutralis place ml suspens cell cell ml cell cultur medium serum free fmd antibodi ad plate seal plate incub monolay confluent hour cpe suffici advanc hour microscop read test time final microscop read made plate fix stain macroscop read instanc formol salin methylen blue control control test includ homolog antiserum titr cell control serum toxic control medium control viru titrat actual amount viru test calcul interpret well evid cpe consid infect neutralis titr express reciproc final dilut serum present serum viru mixtur end point estim spearman karber method karber archiv fuer experimentel pathologi und pharmokologi test consid valid actual amount viru test tcid titr refer serum twofold expect titr estim mode previou titrat control limit test repeat end point titr neg detect quantif antibodi elisa carri protocol reagent rabbit antisera antigen type foot mouth diseas viru fmdv predetermin optimum concentr carbon bicarbon buffer ph antigen prepar select strain viru grown monolay bhk cell unpurifi supernat pretitr protocol serum give dilut addit equal volum pbst phosphat buffer salin tween phenol red indic give optic densiti read virus inactiv pbst diluent guinea pig antisera prepar inocul guinea pig antigen serotyp predetermin optimum concentr prepar pbst normal bovin serum normal rabbit serum rabbit anti guinea pig immunoglobulin conjug horseradish peroxidas predetermin optimum concentr pbst normal bovin serum normal rabbit serum test sera dilut pbst procedur elisa plate coat μl rabbit antivir sera overnight humid chamber room temperatur fifti microlitr duplic twofold seri test serum start prepar bottom multiwel plate carrier plate fifti microlitr constant dose antigen ad mixtur left overnight addit antigen reduc start serum dilut elisa plate wash time pbst fifti microlitr serum antigen mixtur transfer carrier plate rabbit serum coat elisa plate incub hour rotari shaker wash μl guinea pig antiserum antigen point ad plate incub hour rotari shaker plate wash μl rabbit anti guinea pig immunoglobulin conjug horseradish peroxidas ad plate incub hour rotari shaker plate wash μl orthophenylen diamin ad reaction stop minut plate read spectrophotometr nm elisa reader link microcomput control antigen well serum antigen dilut pbst duplic twofold dilut seri homolog bovin refer antiserum duplic twofold dilut seri neg bovin serum interpret antibodi titr express final dilut test serum give od record viru control well test serum absent titr excess consid posit refer hamblin barnett itr hedger rs quot enzym link immunosorb assai elisa detect antibodi foot mouth diseas viru develop method elisa quot journal immunolog method aujeszki diseas ajd serum neutralis test carri protocol serum sera heat inactiv minut procedur constant viru vari serum neutralis test microtitr plate emploi vero sensit cell system aujeszki diseas viru tcid ml inactiv undilut serum sampl mix equal volum ml viru suspens viru serum mixtur incub hour microtitr plate cell ad cell concentr form complet monolay hour control viru infect assai ii serum toxic control iii uninocul cell cultur control iv refer antisera interpret result neutralis test titr viru test record dai incub serum titr undilut serum consid neg test recognis frame commiss decis ec addit guarante aujeszki diseas pig destin part territori commun transmiss gastroenter tge serum neutralis test carri protocol serum sera heat inactiv minut procedur constant viru vari serum neutralis test microtitr plate emploi dog tumour cell sensit cell system tge viru tcid ml inactiv undilut serum sampl mix equal volum ml viru suspens viru serum mixtur incub minut microtitr plate cell ad cell concentr form complet monolay hour cell receiv ml cell suspens control viru infect assai ii serum toxic control iii uninocul cell cultur control iv refer antisera interpret result neutralis test titr viru test record dai incub serum titr final dilut consid neg undilut serum sampl toxic tissu cultur sera dilut test equival final dilut serum serum titr final dilut consid neg case swine vesicular diseas svd test swine vesicular diseas svd carri commiss decis ec classic swine fever csf test classic swine fever csf carri commiss decis ec perform test csf follow guidelin set oie manual standard diagnost test vaccin chapter sensit specif serolog test csf carri nation laboratori qualiti assur scheme place test emploi shown recognis rang weak strong posit refer sera detect antibodi earli phase convalesc annex ii fresh meat part list countri part thereof gt tabl gt specif condit refer column quot quot geograph time restrict gt tabl gt quot quot categori restrict offal authoris bovin diaphragm masset muscl part model veterinari certif model quot bov quot model veterinari certif fresh meat domest bovin anim bo tauru bison bison bubalu bubali cross breed quot por quot model veterinari certif fresh meat domest porcin anim su scrofa quot ovi quot model veterinari certif fresh meat domest sheep ovi ari goat capra hircu quot equ quot model veterinari certif fresh meat domest equin anim equu caballu equu asinu cross breed quot ruf quot model veterinari certif fresh meat farm domest anim suida solip quot ruw quot model veterinari certif fresh meat wild domest anim suida solip quot suf quot model veterinari certif fresh meat farm domest suida quot suw quot model veterinari certif fresh meat wild domest suida quot eqw quot model veterinari certif fresh meat wild domest solip sg supplementari guarante quot quot guarante matur ph measur debon fresh meat exclud offal certifi model certif bov point ovi point ruf point ruw point quot quot guarante matur trim offal model certif bov point quot quot guarante laboratori test classic swine fever carcas fresh meat certifi model certif suw point obtain quot quot guarante swill feed hold anim fresh meat certifi model certif por point obtain quot quot guarante tuberculosi test anim fresh meat certifi model certif bov point obtain quot quot guarante matur debon fresh meat exclud offal certifi model certif bov point ovi point ruf point ruw point note veterinari certif produc export countri base model appear part annex ii layout model correspond meat concern number order appear model attest requir countri case supplementari guarante requir export countri part thereof separ uniqu certif provid meat export singl territori appear column part annex ii consign destin transport railwai wagon lorri aircraft ship origin certif consist singl page side text requir form page need part integr indivis drawn offici languag eu member state inspect border post carri eu member state destin member state languag accompani offici translat reason identif item consign schedul point model certif addit page attach certif page consid form part origin certif applic signatur stamp certifi offici veterinarian page certif includ addit schedul refer compris page page number page number total number page bottom bear code number certif design compet author top origin certif complet sign offici veterinarian compet author export countri ensur principl certif equival laid council direct ec colour signatur print rule appli stamp emboss watermark origin certif accompani consign eu border inspect post gt pic file quot en tif quot gt gt pic file quot en tif quot gt gt pic file quot en tif quot gt gt pic file quot en tif quot gt gt pic file quot en tif quot gt gt pic file quot en tif quot gt gt pic file quot en tif quot gt gt pic file quot en tif quot gt gt pic file quot en tif quot gt gt pic file quot en tif quot gt gt pic file quot en tif quot gt gt pic file quot en tif quot gt gt pic file quot en tif quot gt gt pic file quot en tif quot gt gt pic file quot en tif quot gt gt pic file quot en tif quot gt gt pic file quot en tif quot gt gt pic file quot en tif quot gt gt pic file quot en tif quot gt gt pic file quot en tif quot gt gt pic file quot en tif quot gt gt pic file quot en tif quot gt gt pic file quot en tif quot gt gt pic file quot en tif quot gt gt pic file quot en tif quot gt gt pic file quot en tif quot gt quot annex quot annex descript territori countri authoris export commun gt tabl gt annex ii anim public health condit appear model veterinari certif request gt tabl gt quot