commiss regul ec decemb lai commun refer method analysi spirit drink commiss european commun regard treati establish european commun regard council regul eec lai gener rule definit descript present spirit drink amend act access austria finland sweden articl thereof articl regul eec adopt method analys spirit drink refer method ensur complianc regul eec commiss regul eec april lai detail implement rule definit descript present spirit drink amend regul ec offici control take place event disput adopt describ commun analyt refer method gener recognis method order account scientif advanc differ equip offici laboratori method base principl measur refer method annex hereto permit respons laboratori director method offer adequ guarante result meet criteria set annex council direct eec decemb introduct commun method sampl analysi monitor foodstuff intend human consumpt shown variat accuraci repeat reproduc result obtain limit obtain refer method regul condit met analyt method allow import case disput method replac refer method measur provid regul accord opinion implement committe spirit drink adopt regul articl commun refer method analysi spirit drink ensur complianc regul eec regul eec offici control take place event disput set annex hereto articl notwithstand indent articl analyt method permit respons director laboratori condit accuraci precis repeat reproduc method equival relev refer analyt method annex articl commun analyt refer method laid detect quantif substanc contain spirit drink method analyt method valid internation recognis procedur meet criteria set annex direct eec analyt method conform recommend standard intern organis standardis iso analyt method recognis gener assembl intern vine wine offic oiv publish offic absenc method reason accuraci repeat reproduc analyt method approv member state concern suitabl analyt method articl purpos regul quot repeat limit quot equal absolut differ test result obtain repeat condit oper apparatu laboratori short interv time expect probabl iso quot reproduc limit quot equal absolut differ test result obtain reproduc condit oper apparatu laboratori expect probabl iso quot accuraci quot close agreement test result accept refer iso articl regul enter forc seventh dai public offici journal european commun appli januari regul bind entireti directli applic member state brussel decemb commiss franz fischler member commiss oj oj oj oj annex descript analyt refer method gt tabl gt determin alcohol strength volum spirit drink introduct refer method includ appendic appendix prepar distil appendix ii measur densiti distil scope method suitabl determin real alcohol strength volum spirit drink norm refer iso water analyt laboratori specif test method term definit refer temperatur refer temperatur determin alcohol strength volum densiti specif graviti spirit drink note term quot quot reserv determin densiti alcohol strength volum express temperatur refer temperatur densiti densiti mass unit volum vacuo spirit drink express kilogram cubic metr symbol specif graviti specif graviti ratio express decim number densiti spirit drink densiti water temperatur denot symbol simpli possibl confus characterist measur assai certif defin symbol note obtain specif graviti densiti gt refer graphic gt gt refer graphic gt densiti water real alcohol strength volum real alcohol strength volum spirit drink equal number litr ethyl alcohol contain water alcohol mixtur densiti alcohol spirit distil refer valu alcohol strength volum vol versu densiti water alcohol mixtur intern tabl adopt intern legal metrolog organis recommend gener equat relat alcohol strength volum densiti water alcohol mixtur temperatur page chapter quot alcohol strength volum quot annex commiss regul eec oj manual analysi method oiv note liqueur crème difficult measur volum accur sampl weigh alcohol strength calcul mass convers formula gt pic file quot en ep quot gt asm alcohol strength mass alcohol kg principl distil alcohol strength volum distil determin pycnometri electron densimetri densimetri hydrostat balanc appendix prepar distil scope method suitabl prepar distil determin real alcohol strength volum spirit drink principl spirit distil separ ethyl alcohol volatil compound extract matter substanc distil reagent materi anti bump granul concentr antifoam emuls crème liqueur apparatu equip usual laboratori apparatu water bath capabl maintain water bath capabl maintain class volumetr flask ml ml certifi distil apparatu gener requir distil apparatu meet specif number joint strict minimum need ensur system leak tight inclus devic design prevent prime entrain boil liquid vapour regularis distil rate alcohol rich vapour rapid complet condens alcohol vapour collect distil fraction aqueou medium heat sourc suitabl heat diffus prevent pyrogen reaction involv extract matter suitabl distil apparatu shown figur includ part round bottom flask litr standardis ground glass joint rectifi column cm high vigreux column elbow connector approxim cm long straight rim condens west type condens fit vertic cool coil cm long drawn tube take distil bottom graduat collect flask small amount water note apparatu intend sampl ml smaller sampl size ml distil smaller distil flask provid splashhead devic prevent entrain storag test sampl sampl store room temperatur prior analysi procedur preliminari remark distil procedur publish iupac distil apparatu verif apparatu capabl distil ml water alcohol solut concentr close vol loss alcohol vol spirit drink alcohol strength vol measur ml spirit volumetr flask record temperatur liquid maintain standard temperatur pour sampl round bottom flask distil apparatu rins volumetr flask aliquot approxim ml distil water add rins water aliquot content distil flask note ml dilut suffici spirit dry extract litr prevent pyrolysi volum rins water ml dry extract concentr ml dry extract ml dry extract fruit liqueur crème adjust volum proportion sampl volum add anti bump granul antifoam crème liqueur pour ml distil water origin ml volumetr flask hold distil flask cold water bath anise flavour spirit drink distil avoid entrain char occasion agit content flask level distil millimetr calibr mark volumetr flask temperatur distil brought liquid initi temperatur make mark distil water mix distil determin alcohol strength volum appendix ii spirit drink alcohol strength vol measur ml spirit drink ml volumetr flask pour round bottom flask distil apparatu rins volumetr flask time distil water add wash content round bottom distil flask water bring flask content approxim ml pour ml distil water ml volumetr flask hold distil flask cold water bath anise flavour spirit distil agit content occasion level distil millimetr calibr mark ml volumetr flask temperatur distil brought liquid initi temperatur make mark distil water mix distil determin alcohol strength volum appendix ii note alcohol strength volum spirit drink alcohol strength distil appendix ii measur densiti distil method determin real alcohol strength volum spirit drink measur pycnometri principl alcohol strength volum obtain densiti distil measur pycnometri reagent materi analysi state reagent recognis analyt grade water grade defin iso sodium chlorid solut prepar litr weigh sodium chlorid dissolv litr water apparatu equip usual laboratori apparatu analyt balanc capabl read mg thermomet ground glass joint calibr tenth degre thermomet certifi check certifi thermomet pyrex glass pycnomet approxim ml capac fit remov ground glass thermomet pycnomet side tube mm length mm maximum intern diamet end conic ground joint pycnomet iso ml tare bottl extern volum ml pycnomet mass equal mass pycnomet fill liquid densiti sodium chlorid solut thermal insul jacket fit bodi pycnomet note method determin densiti vacuo spirit call twin pan balanc pycnomet tare bottl extern volum cancel effect air buoyanc moment simpl techniqu appli singl pan balanc provid tare bottl weigh monitor air buoyanc time procedur preliminari remark procedur ml pycnomet determin alcohol strength accuraci smaller pycnomet ml calibr pycnomet pycnomet calibr determin paramet tare empti pycnomet volum pycnomet mass water fill pycnomet calibr singl pan balanc determin mass clean dry pycnomet mass water fill pycnomet mass tare bottl weigh clean dry pycnomet fill pycnomet carefulli distil water ambient temperatur fit thermomet carefulli wipe pycnomet dry place thermal insul jacket agit invert contain thermomet temperatur read constant set pycnomet flush upper rim side tube read temperatur carefulli correct inaccuraci temperatur scale weigh water fill pycnomet weigh tare bottl calcul tare empti pycnomet mass air pycnomet note densiti dry air pressur mm hg volum pycnomet gt pic file quot en ep quot gt ft factor temperatur tabl chapter quot densiti specif graviti quot annex regul eec nearest ml mass water pycnomet gt refer graphic gt densiti water note densiti air alcohol strength calcul refer densiti hm custom excis tabl air calibr method twin pan balanc place tare bottl left hand pan clean dry pycnomet collect stopper hand pan balanc place weight pycnomet side gram fill pycnomet carefulli distil water ambient temperatur fit thermomet carefulli wipe pycnomet dry place thermal insul jacket agit invert contain thermomet temperatur read constant accur adjust level upper rim side tube clean side tube fit collect stopper read temperatur carefulli correct inaccuraci temperatur scale weigh water fill pycnomet weight gram make equilibrium calcul tare empti pycnomet mass air pycnomet volum pycnomet gt pic file quot en ep quot gt ft factor temperatur tabl chapter quot densiti specif graviti quot annex regul eec nearest ml mass water pycnomet gt refer graphic gt densiti water determin alcohol strength test sampl singl pan balanc weigh tare bottl weight weigh pycnomet prepar distil appendix weight calcul gt refer graphic gt mass empti pycnomet moment measur dt mass liquid pycnomet dt densiti ml gt pic file quot en ep quot gt express densiti kilogram multipli ρt ρt correct ρt tabl densiti ρt water alcohol mixtur tabl ii appendix ii oiv manual analysi method pp tabl find horizont line temperatur degre immedi smallest densiti ρt tabl differ found densiti calcul densiti ρt spirit temperatur degre temperatur line calcul differ densiti tabl immedi ρt calcul densiti ρt divid differ tabl differ found densiti quotient decim portion alcohol strength integ alcohol strength found top column densiti found dt alcohol strength note altern pycnomet water bath maintain make mark result densiti calcul real alcohol strength alcohol strength tabl identifi tabl give alcohol strength volum vol function densiti water alcohol mixtur intern tabl adopt intern legal metrolog organis recommend method singl pan balanc weigh pycnomet distil prepar part quot mass calcul mass liquid pycnomet quot densiti ml gt pic file quot en ep quot gt express densiti kilogram carri temperatur correct order calcul alcohol strength singl pan balanc method perform characterist precis statist result interlaboratori test data obtain intern method perform studi carri internation agre procedur gt tabl gt gt tabl gt method determin real alcohol strength volum spirit drink measur electron densimetri base reson frequenc oscil sampl oscil cell principl liquid densiti determin electron measur oscil vibrat tube perform measur sampl ad oscil system specif oscil frequenc modifi ad mass reagent materi analysi state reagent recognis analyt grade water grade defin iso aceton ca absolut alcohol dry air apparatu equip usual laboratori apparatu digit displai densimet electron densimet perform measur capabl express densiti ml decim place note densimet perfectli stabl stand insul vibrat temperatur regul densimet perform valid measur cell connect built temperatur regul achiev temperatur stabil note precis set monitor temperatur measur cell import error lead variat densiti order kg sampl inject syring auto sampler procedur calibr densimet apparatu calibr instrument manufactur instruct put servic recalibr regularli check certifi refer standard intern laboratori refer solut base certifi refer standard determin sampl densiti requir prior measur clean dry cell aceton absolut alcohol dry air rins cell sampl inject sampl cell syring autosampl cell complet fill fill oper make air bubbl complet elimin sampl homogen solid particl suspend matter remov filtrat prior analysi read stabilis record densiti alcohol strength displai densimet result densiti calcul real alcohol strength alcohol strength tabl identifi tabl give alcohol strength volum vol function densiti water alcohol mixtur intern tabl adopt intern legal metrolog organis recommend method perform characterist precis statist result interlaboratori test data obtain intern method perform studi carri internation agre procedur gt tabl gt gt tabl gt method determin real alcohol strength volum spirit drink measur densimetri hydrostat balanc principl alcohol strength spirit measur densimetri hydrostat balanc base archimed principl bodi immers liquid receiv vertic upward thrust liquid equal weight liquid displac reagent materi analysi state reagent recognis analyt grade water grade defin iso float clean solut sodium hydroxid prepar ml weigh sodium hydroxid make volum volum ethanol apparatu equip usual laboratori apparatu singl pan hydrostat balanc sensit mg float volum ml special adapt balanc suspend thread diamet exceed mm measur cylind bear level mark float capabl contain complet volum cylind locat mark surfac liquid penetr support thread measur cylind intern diamet mm larger float thermomet temperatur measur probe graduat degre tenth degre calibr weight calibr recognis certifi bodi note twin pan balanc principl chapter quot densiti specif graviti quot annex regul eec procedur float measur cylind clean measur distil water dri soft laboratori paper shed fibr rins solut densiti determin measur made apparatu reach stabil restrict alcohol loss evapor calibr balanc balanc intern calibr system hydrostat balanc capabl calibr weight check offici certifi bodi calibr float fill measur cylind mark doubl distil water water equival puriti microfilt water conduct mω cm temperatur prefer immers float thermomet stir read densiti liquid apparatu correct read equal water measur temperatur control water alcohol solut fill measur cylind mark water alcohol mixtur strength temperatur prefer immers float thermomet stir read densiti liquid alcohol strength apparatu alcohol strength establish equal previous determin alcohol strength note solut alcohol strength calibr float doubl distil water measur densiti distil alcohol strength apparatu pour test sampl measur cylind graduat mark immers float thermomet stir read densiti liquid alcohol strength apparatu note temperatur densiti measur ρt correct ρt tabl densiti ρt water alcohol mixtur tabl ii annex ii oiv manual analysi method pp clean float measur cylind immers float float clean solut measur cylind soak hour spin float period rins copiou amount tap water distil water dry soft laboratori paper shed fibr carri procedur float regularli requir result densiti calcul real alcohol strength alcohol strength tabl identifi tabl give alcohol strength volum vol function densiti water alcohol mixtur intern tabl adopt intern legal metrolog organis recommend method perform characterist precis statist result interlaboratori test data obtain intern method perform studi carri internation agre procedur gt tabl gt gt tabl gt figur distil apparatu measur real alcohol strength volum spirit gt pic file quot en ep quot gt litr round bottom flask standardis spheric ground glass joint cm vigreux rectifi column cm straight rim west condens cm cool coil ii determin total dry extract spirit drink gravimetri scope regul eec method aquavit dry extract limit norm refer iso water analyt laboratori specif test method definit total dry extract total dry matter includ matter volatil physic condit principl weigh residu left evapor spirit boil water bath dry dry oven apparatu equip flat bottom cylindr evapor dish mm diamet boil water bath ml pipett class dry oven desicc analyt balanc accur mg sampl sampl sampl store room temperatur prior analysi procedur pipett ml spirit dry matter previous weigh flat bottom cylindr evapor dish mm diamet hour evapor evapor dish lid boil water bath liquid boil lead loss splatter leav hour directli contact steam boil water bath complet dry place evapor dish dry oven hour evapor dish cool desicc weigh evapor dish content calcul mass residu multipli equal dry extract contain spirit express decim place method perform characterist precis statist result interlaboratori test data obtain intern method perform studi carri internation agre procedur gt tabl gt gt tabl gt iii determin volatil substanc methanol spirit drink iii gener remark definit regul eec set minimum level volatil compound ethanol methanol seri spirit drink rum spirit viticultur origin fruit spirit seri drink level convention consid equival sum concentr volatil acid express acet acid aldehyd express ethan sum ethan acetaldehyd ethan fraction contain diethoxyethan acet higher alcohol propan ol butan ol butan ol methylpropan ol assai individu alcohol methylbutan ol methylbutan ol assai individu alcohol sum ethyl acet convent method measur volatil compound volatil acid mean volatil acid aldehyd ethan acet ethyl acet alcohol mean ga chromatographi gpc ga chromatograph analysi volatil compound ga chromatograph assai volatil compound set prove interest mean determin origin raw materi distil actual condit distil spirit volatil compon aromat compound characterist raw materi obtain alcohol aroma spirit drink special featur prepar spirit compound import evalu requir set regul eec iii ga chromatograph determin volatil congen aldehyd higher alcohol ethyl acet methanol scope method suitabl determin diethoxyethan acet methylbutan ol activ amyl alcohol methylbutan ol isoamyl alcohol methanol methyl alcohol ethyl ethano ethyl acet butan ol butanol butan ol sec butanol methylpropan ol isobutyl alcohol propan ol propanol ethan acetaldehyd spirit drink ga chromatographi method intern standard pentan ol concentr analyt express gram litr absolut alcohol alcohol strength product determin prior analysi spirit drink analys method includ whiski brandi rum wine spirit fruit spirit grape marc spirit norm refer iso water analyt laboratori specif test method definit congen volatil substanc form ethanol ferment distil matur spirit drink principl congen spirit drink determin direct inject spirit drink appropri dilut spirit drink ga chromatographi gc system suitabl intern standard ad spirit drink prior inject congen separ temperatur program suitabl column detect flame ionis detector fid concentr congen determin respect intern standard respons factor obtain calibr chromatograph condit spirit drink analysi reagent materi state reagent puriti greater purchas iso accredit supplier certif puriti free congen test dilut confirm inject individu congen standard test dilut gc condit water grade defin iso acet acetaldehyd store dark amp lt reagent store room temperatur ethanol absolut ca methanol ca propan ol ca methylpropan ol ca accept intern standard pentan ol ca pentan ol ca methylpentan ol ca methyl nonano ca methylbutan ol ca methylbutan ol ca ethyl acet ca butan ol ca butan ol ca acetaldehyd ca acet ca ethanol solut prepar ml ethanol solut pour ml ethanol litr volumetr flask make volum distil water mix prepar storag standard solut procedur valid method standard solut store amp lt prepar freshli monthli basi mass compon solut record nearest mg standard solut pipett reagent ml volumetr flask approxim ml ethanol solut minimis compon evapor make volum ethanol solut mix record weight flask compon ad total final weight content gt tabl gt note prefer add acet acetaldehyd order minimis loss evapor standard solut pipett ml pentan ol suitabl intern standard ml volumetr flask approxim ml ethanol solut make volum ethanol solut mix record weight flask weight pentan ol intern standard ad total final weight content standard solut pipett ml solut ml solut ml volumetr flask approxim ml ethanol solut make volum ethanol solut mix record weight flask compon ad total final weight content standard solut order maintain analyt continu prepar qualiti control standard previous prepar standard pipett ml solut ml volumetr flask approxim ml ethanol solut make volum ethanol solut mix record weight flask compon ad total final weight content standard solut pipett ml solut ml volumetr flask approxim ml ethanol solut make volum ethanol solut mix record weight flask compon ad total final weight content standard solut check linear respons fid separ ml volumetr flask approxim ml ethanol pipett ml solut ml solut make volum ethanol solut mix record weight flask compon ad total final weight content qc standard solut pipett ml standard solut ml standard solut weigh vessel mix record weight flask compon ad total final weight content apparatu equip apparatu capabl measur densiti alcohol strength analyt balanc capabl measur decim place temperatur program ga chromatograph fit flame ionis detector integr data handl system capabl measur peak area peak height ga chromatograph column capabl separ analyt minimum resolut individu compon methylbutan ol methylbutan ol note column gc condit suitabl exampl retent gap mm connect cp wax cb column mm μm film thick stabilis polyethylen glycol carbowax column mm μm film thick column connect press fit connector gt tabl gt retent gap mm connect cp wax cb column mm μm film thick stabilis polyethylen glycol retent gap connect press fit connector gt tabl gt pack column cw carbopak mm gt tabl gt sampl sampl laboratori sampl receipt alcohol strength sampl measur procedur valid method test portion weigh seal weigh vessel record weight pipett ml laboratori sampl vessel record weight msampl add ml standard solut record weight mi shake test materi vigor invers sampl store prior analysi order minimis volatil loss blank test decim place balanc weigh seal weigh vessel record weight pipett ml ml ethanol solut vessel record weight add ml standard solut record weight shake test materi vigor invers sampl store prior analysi order minimis volatil loss preliminari test inject standard solut ensur analyt separ minimum resolut methylbutan ol methylbutan ol calibr calibr check procedur ensur respons linear success analys triplic linear standard solut intern standard integr peak area peak height inject calcul ratio congen plot graph versu concentr ratio congen intern standard linear plot obtain correl coeffici gt pic file quot en ep quot gt determin inject standard solut qc standard solut follow unknown sampl prepar insert qc standard sampl ensur analyt stabil inject standard solut sampl calcul autom system data handl provid data check principl method measur peak area peak height congen intern standard peak respons factor calcul chromatogram inject standard solut calcul respons factor congen equat gt pic file quot en ep quot gt intern standard conc congen concentr congen solut conc concentr intern standard solut sampl analysi equat calcul concentr congen sampl gt pic file quot en ep quot gt msampl weight sampl mi weight intern standard conc concentr intern standard solut rf respons factor calcul equat qualiti control standard solut analysi equat calcul percentag recoveri target congen qualiti control standard gt pic file quot en ep quot gt concentr analyt qc standard calcul equat final present result result convert litr absolut alcohol sampl equat gt pic file quot en ep quot gt densiti kg result quot signific figur maximum decim place absolut alcohol qualiti assur control valid method equat calcul concentr congen qualiti control standard solut prepar procedur equat calcul percentag recoveri target analys result theoret valu congen analysi proce investig made find inaccuraci remedi action method perform characterist precis statist result interlaboratori test tabl give valu compound ethan ethyl acet acet total ethan methanol butan ol propan ol butan ol methyl propan ol methyl butan ol methyl butan ol data obtain intern method perform studi carri internation agre procedur gt tabl gt gt tabl gt gt tabl gt gt tabl gt gt tabl gt gt tabl gt gt tabl gt gt tabl gt gt tabl gt gt tabl gt gt tabl gt gt tabl gt gt tabl gt gt tabl gt gt tabl gt gt tabl gt gt tabl gt gt tabl gt gt tabl gt gt tabl gt gt tabl gt gt tabl gt