commiss regul ec juli amend annex council direct eec commiss european commun regard treati establish european commun regard council direct eec june anim health problem affect intra commun trade bovin anim swine amend commiss regul ec articl paragraph thereof octob scientif committe anim health anim welfar adopt report modif technic annex council direct eec account scientif develop tuberculosi brucellosi enzoot bovin leucosi accord report test tuberculosi carri line manual standard diagnost test vaccin edit offic intern de epizooti oie august oie publish fourth edit manual includ modif descript test tuberculosi januari european director qualiti medicin publish fourth edit european pharmacopoeia includ monogaph tuberculin purifi protein deriv avian bovin amend annex direct eec lai test procedur applic surveil trade purpos commun take account opinion scientif veterinari committe measur provid regul accord opinion stand committe food chain anim health adopt regul articl annex direct eec replac annex present regul articl regul enter forc dai public offici journal european commun regul bind entireti directli applic member state brussel juli commiss david byrn member commiss oj oj sanco annex quot annex tuberculosi identif agent presenc mycobacterium bovi bovi agent bovin tuberculosi clinic post mortem specimen demonstr examin stain smear immunoperoxidas techniqu confirm cultiv organ primari isol medium patholog materi confirm bovi abnorm lymph node parenchymat organ lung liver spleen case anim present patholog lesion sampl retropharyng bronchial mediastin supramammari mandibular mesenter lymph node liver collect examin cultur identif isol carri determin cultur biochem properti polymeras chain reaction pcr emploi detect tuberculosi complex dna analysi techniqu prove faster reliabl biochem method differenti bovi member tuberculosi complex genet fingerprint distinguish strain bovi enabl pattern origin transmiss spread bovi techniqu media standardis interpret result conform oie manual standard diagnost test vaccin fourth edit chapter bovin tuberculosi tuberculin skin test tuberculin ppd purifi protein deriv fulfil standard laid paragraph carri offici tuberculin skin test procedur refer paragraph standard tuberculin bovin avian definit tuberculin purifi protein deriv tuberculin ppd bovin avian prepar obtain heat treat product growth lysi mycobacterium bovi mycobacterium avium appropi capabl reveal delai hypersensit anim sensitis microorgan speci product obtain water solubl fraction prepar heat free flow steam subsequ filter cultur bovi avium appropi grown liquid synthet medium activ fraction filtrat consist protein isol precipit wash dissolv antimicrobi preserv give rise fals posit reaction phenol ad final steril prepar free mycobacteria distribut asept steril tamper proof glass contain close prevent contamin prepar freez dri identif product inject rang grade dose intraderm site suitabl sensitis albino guinea pig weigh reaction form oedemat swell erythema necrosi point inject size sever reaction vari dose unsensitis guinea pig show reaction similar inject test ph ph phenol prepar examin phenol concentr sensitis effect group guinea pig treat materi interfer test occas interv dai inject intraderm guinea pig dose prepar examin equival iu ml dai inject inject dose iu intraderm anim control group guinea pig mass previous receiv inject tuberculin hour inject reaction group significantli toxic guinea pig weigh previous treat materi interfer test inject subcutan guinea pig ml prepar examin observ anim dai abnorm effect occur observ period steril compli test steril prescrib monograph vaccin veterinari edit european pharmacopoeia potenc potenc tuberculin purifi protein deriv bovin avian determin compar reaction produc sensitis guinea pig intraderm inject seri dilut prepar examin produc concentr refer prepar tuberculin bovin avian appropi purifi protein deriv calibr intern unit test potenc sensitis fewer albino guinea pig weigh deep intramuscular inject mg wet mass live bovi strain suspend ml solut sodium chlorid bovin tuberculin suitabl dose inactiv live avium avian tuberculin week sensitis guinea pig shave flank provid space inject site side prepar dilut prepar examin refer prepar isoton phosphat buffer salin ph polysorb fewer dose refer prepar fewer dose prepar examin choos dose lesion produc diamet mm mm alloc dilut randomli site latin squar design inject dose intraderm constant volum ml ml measur diamet lesion hour calcul result test usual statist method assum diamet lesion directli proport logarithm concentr tuberculin test valid fiduci limit error estim potenc estim potenc state potenc bovin tuberculin estim potenc state potenc avian tuberculin state potenc iu ml tuberculin bovin avian storag store protect light temperatur label label state potenc intern unit millilitr quantiti ad substanc freez dri prepar volum reconstitut liquid ad product immedi reconstitut test procedur recognis offici intraderm tuberculin test singl intraderm test test requir singl inject bovin tuberculin intraderm compar test test requir inject bovin tuberculin inject avian tuberculin simultan dose tuberculin inject iu bovin tuberculin iu avian tuberculin volum inject dose exce ml tuberculin test carri inject tuberculin skin neck inject site situat border anterior middl third neck avian bovin tuberculin inject anim site inject avian tuberculin cm crest neck site inject bovin tuberculin cm lower line roughli parallel line shoulder side neck young anim room separ site suffici side neck inject made side neck ident site centr middl neck techniqu tuberculin test interpret reaction techniqu inject site clip cleans fold skin clip area forefing thumb measur callip record dose tuberculin inject method ensur tuberculin deliv intraderm short steril needl bevel edg outward graduat syring charg tuberculin insert obliqu deeper layer skin correct inject confirm palpat small pea swell site inject skin fold thick inject site remeasur hour hour inject record interpret reaction interpret reaction base clinic observ record increas skin fold thick site inject hour inject tuberculin neg reaction limit swell observ increas mm thick fold skin clinic sign diffus extens oedema exud necrosi pain inflamm lymphat duct region lymph node inconclus reaction clinic sign mention observ increas skin fold thick mm mm posit reaction clinic sign mention observ increas mm thick fold skin inject site interpret offici intraderm tuberculin test singl intraderm test posit posit bovin reaction defin paragraph inconclus inconclus reaction defin paragraph neg neg bovin reaction defin paragraph anim inconclus singl intraderm test subject test minimum dai anim neg test deem posit test anim posit singl intraderm test subject intraderm compar test fals posit reaction interfer reaction suspect intraderm compar test establish mainten offici tuberculosi free herd statu posit posit bovin reaction mm greater avian reaction presenc clinic sign inconclus posit inconclus bovin reaction mm greater avian reaction absenc clinic sign neg neg bovin reaction posit inconclus bovin reaction equal posit inconclus avian reaction absenc clinic sign case anim inconclus intraderm compar test subject test minimum dai anim neg test deem posit test offici tuberculosi free herd statu suspend anim herd allow enter intra commun trade time statu anim resolv anim deem inconclus singl intraderm tuberculin test anim deem posit singl intraderm tuberculin test await retest intraderm compar test anim deem inconclus intraderm compar test anim requir commun legisl subject intraderm test prior movement test interpret anim show increas skin fold thick greater mm presenc clinic sign enter intra commun trade enabl detect maximum number infect diseas anim herd region member state modifi criteria interpret test order achiev improv test sensit inconclus reaction refer posit reaction supplementari test enabl detect maximum number infect diseas anim herd region member state authoris emploi gamma interferon assai refer oie manual standard diagnost test vaccin edit chapter bovin tuberculosi addit tuberculin test state institut nation refer laboratori task respons state institut refer laboratori includ paragraph respons offici test tuberculin reagent includ paragraph respect state ensur tuberculin reagent adequ relat standard refer list state institut nation refer laboratori germani paul ehrlich institut pei bundesamt für sera und impstoff langen bundesinstitut für gesundheitlichen verbraucherschutz und veterinärmedizin bereich jena jena belgium institut scientifiqu de la santé publiqu loui pasteur rue juliett wytsman bruxel belgiqu franc laboratoir nation de médicament vétérinair fougèr grand duchi luxembourg institut suppli countri itali istituto superior di sanità rome netherland centraal instituut voor dierziekt control lelystad cidc lelystad lelystad denmark danmark veterinærinstitut bülowsvej dk københavn ireland institut suppli countri unit kingdom veterinari laboratori agenc addleston weybridg greec spain laboratorio de sanidad producción anim de granada portug laboratorio nacion de investigação veterinária lisbon austria bundesanstalt für veterinärmedizinisch untersuchungen mödling finland eläinlääkintä ja elintarviketutkimu laito forskningsanstalten för veterinärmedicin och livsmedel helsinki sweden staten veterinärmedicinska anstalt uppsala quot