commiss direct ec juli establish commun method analysi determin lasalocid sodium feedingstuff text eea relev commiss european commun regard treati establish european commun regard council direct eec juli introduct commun method sampl analysi offici control feedingstuff amend act access austria finland sweden articl thereof direct eec stipul offici control feedingstuff purpos check complianc requir aris law regul administr provis govern qualiti composit carri commun method sampl analysi council direct eec novemb addit feedingstuff amend commiss regul ec april stipul lasalocid sodium content label substanc ad premixtur feedingstuff commun method analysi establish check substanc measur provid direct accord opinion stand committe feedingstuff adopt direct articl member state provid analys conduct view offici control lasalocid content feedingstuff premixtur carri method set annex hereto articl member state bring forc januari law regul administr provis compli provis direct immedi inform commiss thereof appli measur februari member state adopt measur refer direct accompani refer time offici public procedur refer adopt member state articl direct enter forc dai public offici journal european commun articl direct address member state brussel juli commiss franz fischler member commiss oj oj oj annex determin lasalocid sodium sodium salt polyeth monocarboxyl acid produc streptomyc lasaliensi purpos scope method determin lasalocid sodium feedingstuff premixtur limit detect mg kg limit determin mg kg principl lasalocid sodium extract sampl acidifi methanol determin revers phase high perform liquid chromatographi hplc spectrofluorimetr detector reagent potassium dihydrogen phosphat kh po orthophosphor acid orthophosphor acid solut dilut ml orthophosphor acid ml water methyl heptylamin dimethylhexylamin methanol hplc grade hydrochlor acid ml phosphat buffer solut mol dissolv kh po ml water add ml orthophosphor acid ml methyl heptylamin adjust ph orthophosphor acid solut dilut ml water acidifi methanol transfer ml hydrochlor acid ml graduat flask make mark methanol mix solut prepar freshli hplc mobil phase phosphat buffer methanol solut mix ml phosphat buffer solut ml methanol lasalocid sodium standard substanc guarante puriti na sodium salt polyeth monocarboxyl acid produc streptomyc lasaliensi lasalocid sodium stock standard solut μg ml weigh nearest mg mg lasalocid sodium ml graduat flask dissolv acidifi methanol make mark solvent mix solut freshli prepar lasalocid sodium intermedi standard solut μg ml pipett ml stock standard solut ml graduat flask make mark acidifi methanol mix solut prepar freshli calibr solut seri ml graduat flask transfer ml intermedi standard solut make mark acidifi methanol mix solut correspond μg lasalocid sodium ml solut prepar freshli water hplc grade apparatu ultrason bath shake water bath temperatur control membran filter μm hplc equip inject system suitabl inject volum μl liquid chromatograph column mm mm revers phase μm pack equival spectrofluorimet variabl wavelength adjust excit emiss wavelength procedur gener blank feed perform recoveri test blank feed analys check lasalocid sodium interf substanc present blank feed similar type sampl lasalocid sodium interf substanc detect recoveri test recoveri test carri analys blank feed fortifi addit quantiti lasalocid sodium similar present sampl fortifi level mg kg transfer ml stock standard ml conic flask evapor solut approxim ml add blank feed mix leav minut mix time proceed extract step altern blank feed similar type sampl recoveri test perform mean standard addit method case sampl analys fortifi quantiti lasalocid sodium similar present sampl sampl analys unfortifi sampl recoveri calcul subtract extract feedingstuff weigh nearest sampl ml conic flask stopper add ml acidifi methanol pipett stopper loos swirl dispers place flask ultrason bath approxim minut remov cool room temperatur stand hour suspend matter settl filter aliquot portion μm membran filter suitabl vessel proce hplc determin premixtur weigh nearest unground premix ml graduat flask add ml acidifi methanol swirl dispers place flask content ultrason bath approxim minut remov cool room temperatur dilut mark acidifi methanol mix stand hour suspend matter settl filter aliquot portion μm membran filter dilut volum clear filtrat acidifi methanol produc final test solut μg ml lasalocid sodium proce hplc determin hplc determin paramet condit offer guidanc condit provid yield equival result gt tabl gt check stabil chromatograph system inject calibr solut μg ml time constant peak height area retent time achiev calibr graph inject calibr solut time determin peak height area concentr plot calibr graph peak height area ordin concentr μg ml abscissa sampl solut inject sampl extract obtain time volum calibr solut determin peak height area lasalocid sodium peak calcul result peak height area produc inject sampl solut determin concentr lasalocid sodium μg ml refer calibr graph feedingstuff lasalocid sodium content mg kg sampl formula gt refer graphic gt lasalocid sodium concentr sampl solut μg ml volum sampl extract ml mass test portion premixtur lasalocid sodium content mg kg sampl formula gt refer graphic gt lasalocid sodium concentr sampl solut μg ml volum sampl extract ml dilut factor mass test portion valid result ident method base spectrofluorimetri subject interfer uv detect ident analyt confirm chromatographi chromatographi sampl extract fortifi addit amount calibr solut amount ad lasalocid sodium similar amount lasalocid sodium found sampl extract height lasalocid sodium peak enhanc extent base amount lasalocid sodium ad dilut extract peak width half height origin peak width produc unfortifi sampl extract repeat differ result parallel determin carri sampl exce rel higher lasalocid sodium content mg kg mg kg mg kg lasalocid sodium content mg kg mg kg rel higher lasalocid sodium content mg kg recoveri fortifi blank feed sampl recoveri fortifi premixtur sampl recoveri result collabor studi collabor studi arrang premixtur sampl feed sampl analys laboratori duplic analys perform sampl result tabl gt tabl gt number laboratori number singl result sr standard deviat repeat sr standard deviat reproduc cvr coeffici variat repeat cvr coeffici variat reproduc analyst