commiss regul eec januari amend regul eec characterist oliv oil oliv residu oil relev method analysi commiss european commun regard treati establish european econom commun regard council regul eec septemb establish common organ market oil fat amend regul eec articl thereof commiss regul eec amend regul eec defin characterist type oliv oil oliv residu oil relev method analysi regul eec amend addit note chapter combin nomenclatur set annex council regul eec juli tariff statist nomenclatur common custom tariff amend commiss regul eec view experi gain method analysi adapt set greater precis case appar error crept regul eec account studi period member state test scientif valid nation analysi method extend develop research characterist oliv oil defin regul eec adjust ensur puriti product market provid relev method analysi order permit introduct mean need appli method entri forc defer month regul eec amend measur provid regul accord opinion manag committe oil fat adopt regul articl regul eec amend paragraph articl decemb replac februari articl replac articl addit note chapter combin nomenclatur set annex council regul eec replac text set annex xiv regul oj annex amend set annex regul articl regul enter forc st dai public offici journal european commun point annex appli juli oliv oil packag date regul bind entireti directli applic member state brussel januari commiss rené steichen member commiss oj oj oj oj oj oj annex list annex titl annex iv chang determin wax content capillari column ga liquid chromatographi summari titl annex xiii proof refin place replac neutral decolor oliv oil laboratori tabl annex replac tabl type acid meq peroxid meq kg halogem solvent mg kg wax mg kg satur fatti acid posit triglycerid erythiodiol uvaol trilinolein cholesterol brassicasterol campesterol stigmasterol betasitosterol delta stigmasterol total sterol mg kg extra virgin oliv oil camp virgin oliv oil camp ordinari virgin oliv oil camp virgin lampant oliv oil refin oliv oil camp oliv oil camp crude oliv residu oil refin oliv residu oil camp oliv residu oil camp maximum minimum upper limit compound detect electron captur detector compon detect individuella upper limit mg kg delta stigmastadianol clerosterol sitosterol sitostanol delta avamesterol delta stigmastadienol note oil reject characterist li limit laid footnot ad tabl annex purpos establish puriti exce limit categori concern determin passag alumina annex ii point fine word calcul replac calcul annex iv point word seed oil delet annex iv point sentenc replac solut volum hexan ethyl ether introduc develop chamber approxim depth cm special case volum eluent mixtur benzen aceton obtain good band separ annex iv point number replac word squar millimetr delet appendix annex iv figur replac figur chromatogram alcohol fraction virgin oliv oil eicosanol decosanol tricosanol tetracosanol pentacosanol hexacosanol heptacosanol octacosanol annex iv replac text diagram annex iv determin wax content capillari column ga liquid chromatographi scope method describ procedur determin wax content fat oil test condit distinguish oliv oil obtain press obtain extract oliv pomac oil principl addit suitabl intern standard fat oil fraction chromatographi hydrat silica gel column recoveri fraction elut test condit polar triglycerid direct analysi capillari column ga liquid chromatographi apparatu ml erlenmey flask glass column chromatographi mm intern diamet cm long suitabl ga liquid chromatograph capillari column equip system direct introduct column compris thermostat control oven column capabl maintain desir temperatur cold injector direct introduct column flame ioniz detector convert amplifi record integr capabl work convert amplifi rate respons variabl paper speed capillari column glass fuse silico long mm intern diamet intern cover se se liquid equival uniform thick mm microsyring facil column inject capac ml equip case harden needl reagent silica gel mesh articl merck place gel oven hour cool add water shake homogen slurri dark hour prior tu hexan chromatographi ethyl ether chromatographi heptan chromatographi standard solut lauryl arachid hexan intern standard carrier ga hydrogen pure ga liquid chromatographi auxiliari gase hydrogen pure ga liquid chromatographi air pure ga liquid chromatographi procedur separ wax friction prepar chromatograph column suspend silica gel hydrat anhydr hexan introduc column settl spontan complet settl aid electr shaker make chromatograph band homogen percol ml hexan remov impur column chromatographi weigh mg sampl ml flask add suitabl amount intern standard depend assum wax content add mg lauryl arachid case oliv oil mg case oliv pomac oil transfer prepar sampl chromatograph column prepar aid ml portion hexan solvent flow mm upper level absorb start chromatograph elut collect ml hexan ethyl ether mixtur flow drop second ml minut dry result fraction rotari evapor solvent elimin remov ml solvent weak current nitrogen add ml heptan ga liquid chromatograph analysi preliminari procedur condit column fit column ga liquid chromatograph connect inlet port column system outlet port detector check ga liquid chromatographi apparatu oper ga loop detector record effici column time advis condit run light flow ga column switch ga liquid chromatographi apparatu gradual heat temperatur oper temperatur note maintain temperatur hour regul apparatu oper condit regul ga flow light flame connect electron record regul oven temperatur column regul detector record signal sensit high requir perform analysi base line linear peak kind deviat neg rectilinear deviat column connect correct posit deviat column properli condit note condit temperatur time maximum temperatur eluent emploi choic oper condit oper condit gener column temperatur rise minut program increas minut detector temperatur linear speed carrier ga hydrogen cm sec instrument sensit time minimum attenu record sensit mv bottom scale paper speed cm hour amount inject ml solut condit modifi suit characterist column ga liquid chromatograph apparatu order obtain chromatgram meet condit retent time intern standard minut repres peak wax lie bottom scale determin peak integr paramet obtain correct evalu peak area consid perform analysi ml solut aid ml micri syring draw back piston needl empti introduc needl inject system inject quickli second second gentli extract needl perform record wax complet elut base line satisfi requir condit peak identif identifi peak retent time compar mixtur wax retent time analys condit figur wax chromatogram virgin oliv oil quantit analysi determin area peak intern standard aliphat ester aid integr determin wax content ester mg kg fat formula ester mg kg ax ms ax area peak ester area lauryl arachid peak ms mass lauryl arachid ad milligram mass sampl determin gram express result give wax content sum content mg kg fat appendix determin linear ga speed inject ml methan propan ga liquid chromatograph apparatu adjust normal oper condit measur time ga take run column moment inject peak emerg tm linear speed cm sec formula tm length column cm tm time measur second figur wax chromatogram virgin oliv oil intern standard ester ester ester ester ester ester ester annex point replac annex point subparagraph seed oil delet annex point subparagraph anim veget oil fat replac oil annex point fine ad betulinol cholestanol annex point squar millimetr delet annex vi point squar millimetr delet annex ix point replac chromatographi column upper part mm length diamet mm lower part mm length diamet approxim mm annex ix point indent delet annex xiii titl proof refin place replac neutral decolor oliv oil laboratori annex xiv replac annex xiv addit note chapter combin nomenclatur no cover oil deriv sole treatment live analyt characterist acid sterol composit tabbl fatti acid composit percentag total fatti acid tabl ii sterol composit percentag total sterol myrist acid linolen acid arachid eicosano acid behen acid lignocer acid cholesterol brassicasterol campesterol stigmasterol lt campesterol betasitosterol delta stigmastzerol minimum maximum condit valid virgin lampant oil subhead oliv residu oil subhead delta stigmastadienol chlerosterol beta sitosterol sitostanol delta avenasterol delta stigmastadienol no cover chemic alter oliv oil esterifi oliv oil mixtur oliv oil oil presenc esterifi oliv oil oil ascertain method set annex viii regul eec subhead cover oliv oil defin section ii belov obtain sole mechan physic mean condit thermal condit lead deterior oil undergon treatment wash decant centrifug filtrat oil deriv oliv solvent fall head purpos subhead quot virgin lampant oliv oil quot acid mean oliv oil aliphat alcohol content exceed mg kg erythrod uvaol content exceed content satur fatti acid posit triglycerid exceed sum transol isom lower sum translinol translinolen isom lower characterist period number exceed meq kg ii content volatil halogen solvent exceed mg kg solvent iii extinct coeffici higher treatment oil activ alumina higher point fact oil free fatti acid content express oleic acid passag activ alumina accord method set annex ix regul eec extinct coeffici higher neutral decolor laboratori accord method set ou annex xiii aforement regul characterist ak extinct coeffici higher extinct coeffici variat delta nm region higher higher delta km km km km extinct coeffici wavelength peak absorpt curv nm region km en km extinct coeffici wavelength nm lower higher km wavelength iv organolept organolept characterist includ detect defect exceed limit accept panel test score lower accord annex xii regul eec ii purpos subhead quot virgin oil quot mean oliv oil characterist acid content express oleic acid exceed peroxid number exceed meq activ kg aliphat alcohol content exceed mg kg content volatil halogen solvent exceed mg kg exceed mg kg solvent extinct coeffici higher treatment oil activ alumina higher extinct coeffici variat delta nm region higher organolept characterist includ detect defect limit accept panel test score higher accord annex xii regul eec erythrodiol uvaol content exceed content satur fatti acid posit triglycerid exceed sum transol isom lower sum translinol translinolen isom lower subhead cover oliv oil obtain treatment oliv oil fall subhead blend virgin oliv oil characterist acid content express oleic acid exceed aliphat alcohol content exceed mg kg extinct coeffici higher higher treatment sampl activ alumina higher extinct coeffici variat delta nm region higher higher erythrodiol uvaol content exceed content satur fatti acid posit triglycerid exceed sum transol isomer lower sum translinol translinolen isomer lower purpos subhead quot crude oil quot mean oil oliv residu oil characterist acid content express oleic acid greater erythrodiol uvaol content exceed content satur fatti acid posit triglycerid exceed sum transol isom lower sum translinol translinolen isom lower subhead cover oil obtain treatment oil fallin subhead blend virgin oliv oil oil characterist oil refer addit note oil fall subhead content satur fatti acid posit triglycerid exceed sum transol isom lower sum translinol translinolen isom lower subhead cover residu result treatment fatti substanc oil iodin index determin accord metod laid annex xvi regul eec lower higher residu result treatment fatti substanc oil iodin index lower higher peadk area repres retent volum beta sitosterol determin accord annex regul eec total sterol peak area delta stigmastadienol chlerosterol beta sitosterol sitostanol delta avenasterol delta stigmastadienol analyt method determin characterist product refer laid annex regul eec