commiss decis april approv test detect antibodi bovin brucellosi framework council direct eec notifi document number text eea relev ec commiss european commun regard treati establish european commun regard council direct eec june health problem affect intra commun trade bovin anim swine amend direct ec articl thereof bovin anim intend intra commun trade regard bovin brucellosi hold offici free bovin brucellosi addit test dai dispatch serum agglutin test test approv stand veterinari committe procedur adopt relev protocol accord articl direct eec commiss acord procedur articl basi opinion scientif veterinari committe updat amend annex chapter ii adapt scientif develop commiss receiv final report scientif committe anim health anim welfar modif technic annex direct eec account scientif develop tuberculosi brucellosi enzoot bovin leucosi report scientif committe recommend preferenti elisa test complement fixat test buffer brucella antigen test detect antibodi bovin brucellosi carri inter alia sampl blood individu bovin anim recommend procedur line internation recognis standard set oie manual standard edit chapter appear recognis certif purpos brucellosi test result produc elisa test complement fixat test buffer brucella antigen test test carri accord approv protocol sampl blood individu identifi bovin anim dai prior certif test anim intra commun trade pend updat technic annex chapter ii accord articl direct eec elisa test report scientif committe complement fixat test buffer brucella antigen test annex direct approv brucellosi test certif purpos provid articl anim health certif annex model measur provid decis accord opinion stand veterinari committe adopt decis articl test detect antibodi bovin brucellosi approv certif purpos refer articl direct eec complement fixat test buffer brucella antigen test carri accord provis set annex direct eec elisa test carri accord provis set annex decis articl purpos refer articl direct eec test refer articl decis test column quot test quot tabl point indent point section annex model health certif direct eec articl decis address member state brussel april commiss david byrn member commiss oj oj doc sanco annex enzym link immunosorb assai elisa elisa test refer manual standard diagnost test vaccin offic intern de epizooti oie edit chapter indirect elisa specif igg smooth lipopolysaccharid competit inhibit elisa monoclon antibodi specif chain polysaccharid posit smooth lipopolysaccharid standard refer sera elisa strong posit weak posit neg oie standard veterinari laboratori agenc weybridg unit kingdom techniqu standardis interpret result conform oie manual standard edit chapter elisa test test plasma serum detect oie posit weak posit refer sera definit diagnost threshold elisa test redefin term design oie sera line state oie manual standard sera intern primari refer standard nation secondari refer standard establish test member state work standard calibr sampl serum plasma pool number sampl includ pool oie weak posit standard serum detect posit dilut neg sera plasma number sampl make pool upper limit determin nation refer laboratori take account test adequ detect evid infect singl anim group anim sampl serum plasma pool elisa test certif purpos pool sampl serum plasma carri test result undoubtedli relat individu anim includ pool confirmatori test carri sampl serum plasma individu anim