Tunable Stochastic Pulsing in the Escherichia coli Multiple Antibiotic Resistance Network from Interlinked Positive and Negative Feedback Loops
[['Antimicrobial drug resistance has been studied extensively due to its clinical importance .', 'Traditionally , research has focused on heritable genetic mechanisms , but transient mechanisms , where only a subset of the population expresses resistance genes , are beginning to receive attention for their role in the recalcitrance of chronic infections [1] .', 'Examples of transient resistance include bacterial persistence , inducible expression of antibiotic efflux pumps , and biofilm formation [1]–[3] .', 'Although these mechanisms can provide resistance or tolerance to a broad spectrum of chemicals , they are often taxing to the cell , slowing growth or utilizing resources [4] , [5] .', 'Importantly , transient resistance can occur within an isogenic population , where phenotypic variation can provide diversity to hedge against catastrophic events due to unpredictable fluctuations in the environment by insuring that some fraction of the population is always in a resistant state [6]–[11] .', 'MarA , the multiple antibiotic resistance activator , is a global regulator of resistance genes .', 'It is conserved across enteric bacteria including Klebsiella , Salmonella , Escherichia , Enterobacter , and Shigella species , but is best studied in Escherichia coli [12] .', 'Bulk population studies have shown that MarA plays an important role in multidrug tolerance by inducing expression of over 40 genes implicated in antibiotic resistance [13]–[19] .', 'Examples include the AcrAB multidrug efflux pump; micF , an antisense RNA that represses expression of the outer membrane porin OmpF; SodA , a manganese-containing superoxide dismutase; and the outer membrane channel TolC [15] , [20] , [21] .', 'Expression of MarA is inducible , providing increased resistance in response to a sensed compound .', 'As shown in Fig . 1A , marA is arranged in an operon with two other genes: marR , the multiple antibiotic resistance repressor and marB , which does not play a role in regulation [14] .', 'The marRAB operon is activated by monomeric MarA , which binds to a single site upstream of the −35 site , and is repressed by dimeric MarR ( denoted MarR2 ) , which binds to two sites , one between the −10 and −35 sites and one downstream of the transcriptional start site of the operon [14] .', 'A variety of chemicals including phenolic compounds , uncoupling agents , redox-cycling compounds , and aromatic acid metabolites can activate transcription of marRAB [12] , [22] , [23] .', 'Of the known inducers of marRAB , the weak aromatic acid salicylate is the best studied and is known to bind directly to MarR [24] , [25] .', 'Upon addition of 5 mM salicylate , transcription of marRAB increases 21-fold [19] .', 'Not all genes in the mar regulon are activated by the same MarA concentrations , suggesting that a graded response is possible with less costly genes expressed first and more burdensome genes expressed only once high MarA levels are reached [15] , [26] .', 'The regulatory network controlling MarA consists of interlinked positive and negative feedback loops ( Fig . 1A ) .', 'We asked what role these opposing actions play in controlling the dynamics of MarA .', 'Recent studies have shown that interlinked positive and negative feedback can produce a wide range of dynamic behaviors .', 'Examples include robust oscillations , bistability , monostability , or stochastic pulsing [27]–[29] .', 'Several synthetic oscillators have been constructed using interlinked feedback [30]–[32] and it has also been shown to be a common feature in many natural examples of biological oscillators [28] .', 'Stochastic pulsing is emerging as an important feature in gene regulation , regulating competence , sporulation , and stress response in Bacillus subtilis [33]–[36] , persistence in bacteria [37] , [38] , calcium stress response and glucose repression in Saccharomyces cerevisiae [39] , and virulence factors in bacteria [11] , [40]–[44] .', 'More generally , phenotypic diversity within a population has been shown to increase the net growth rate under uncertain environments [6]–[11] .', 'Although bulk population studies have demonstrated that MarA expression can be induced by inhibition of the negative feedback loop , we asked what role the opposing interlinked loops play and how these effects are manifested at the single-cell level .', 'To study this , we developed a stochastic model of the marRAB network .', 'Our findings suggest that the interlinking of positive and negative feedback can produce stochastic pulses in MarA expression when the system is uninduced .', 'Induction with salicylate leads to elevated levels of MarA and decreased variability .', 'By comparing the native network with a reduced noise variant computationally , we show that stochastic pulsing can act as a bet hedging mechanism to insure that some fraction of the population is always expressing resistance genes .', 'The combination of stochastic pulsing and inducible non-noisy expression of MarA can serve to tune the stochasticity of the system to hedge against environmental uncertainty , while allowing for a deterministic response when a stressor is sensed .'], ['We developed a stochastic model to study MarA expression dynamics .', 'In the model , protein production is the result of a series of single random events [45] , including reactions for transcription , translation , and folding of MarA and MarR , dimerization of MarR to MarR2 , MarA and MarR2 association and dissociation events at the marRAB promoter , MarR2 inhibition by salicylate , and mRNA and protein degradation .', 'Reaction rates and constants were drawn from the literature using experimentally derived values ( Methods , Table S1 ) and simulations were conducted using the Gillespie stochastic simulation algorithm [46] .', 'We first asked how the dynamics of MarA expression change with and without induction at the single-cell level .', 'Bulk population studies have shown that MarA expression can be induced [19] , [23] , however it is not clear whether these population-level results obscure more complex dynamics in individual cells .', 'Using a stochastic computational model , we observed distinct pulses in expression of MarA and MarR2 in the absence of induction ( Fig . 1B ) .', 'The pulses are caused by brief periods when both MarR2 molecules dissociate from the marRAB promoter and MarA binds , initiating expression of the marRAB genes .', 'This is terminated when one or two copies of MarR2 bind to the operator , shutting down transcription , and resulting in a pulse in the expression of marRAB genes .', 'Stochastic pulsing has been shown experimentally for several bacterial systems [6]–[11] , [33]–[38] , [40]–[44] .', 'The phenomenon observed here is consistent with data from other well-characterized repressed systems such as the lactose [47] , tryptophan [48] , and arabinose [49] operons , where a transcriptional burst occurs when the repressor randomly dissociates from its binding sites .', 'In contrast to the uninduced system , our simulations show elevated levels of MarA expression when induced , but lack the pulsing behavior observed in the uninduced state ( Fig . 1C ) .', 'In the presence of a harmful compound , constant , high MarA expression would allow the cell to counteract the noxious effects of a stressor without dipping into a state of low tolerance or rising into a regime with unnecessary cost .', 'When induced , the marRAB promoter spends most of the time in an active state with MarA bound and no MarR2 present , resulting in reduced noise and elevated expression of MarA .', 'To further clarify the mechanism behind the pulsing behavior , we analyzed the corresponding deterministic system , finding a single stable fixed point for all values of salicylate ( Fig . S1 ) .', 'We hypothesized that the stochastic pulses in MarA observed in the uninduced system were caused by the interlinked positive and negative feedback loops that control expression of the marRAB operon .', 'To study the relationship between the feedback loops and noise dynamics we compared four variations on the marRAB operon model ( Fig . 2A ) :', '( i ) Wildtype , which includes the complete operator with all binding sites intact;', '( ii ) Only Positive , which eliminates both MarR2 binding sites , leaving only the positive feedback loop;', '( iii ) Only Negative , which eliminates the MarA binding site , leaving the negative feedback loop; and', '( iv ) No Feedback , which removes both feedback loops so that the marRAB operon is constitutively expressed .', 'To allow for a controlled comparison between the four networks , we fixed the mean expression of MarA such that it was the same for all networks when the systems were uninduced .', 'Our findings show that stochastic pulsing is the result of the interaction between the positive and negative feedback loops .', 'We tested the four network variants to quantify how the individual loops influenced the dynamics of MarA ( Fig . 2B ) .', 'In the No Feedback variant expression is constitutive and low levels of noise come from small fluctuations in the birth and death of mRNA and proteins .', 'In the Only Positive case the random fluctuations in MarA levels are amplified .', 'Random increases in MarA lead to further elevated levels of MarA due to positive feedback , while fluctuations that decrease protein levels lower the probability of expression , leading to slow fluctuations in MarA expression .', 'In the Only Negative variant we observe transcriptional bursting when both MarR2 molecules dissociate from the promoter , but because the system lacks positive feedback , bursts in expression are not amplified .', 'Finally , in the Wildtype variant transcriptional bursts created by negative feedback are amplified by positive feedback , since MarA levels increase faster than MarR2 levels [19] and the presence of MarA decreases the apparent binding rate of MarR2 [16] , likely due to steric hindrance [50] .', 'Thus , stochastic pulsing is caused by the combination of positive and negative feedback loops , where the negative feedback loop produces pulses and the positive feedback loop serves to amplify them .', 'To analyze the contributions of the two feedback loops in the presence of increasing levels of the inducer salicylate , we measured the coefficient of variation and noise strength of MarA for each system ( Figs . 2C and D ) .', 'The coefficient of variation ( CV ) is the standard deviation divided by the mean .', 'It measures the relative variation in the system , however decreases in CV can be the result of either decreased noise or increased mean [7] .', 'Therefore , we also considered noise strength as a measure of variability .', 'Noise strength is defined as the variance divided by the mean ( also known as the Fano factor ) ; higher noise strengths imply that the variability is high relative to the mean , giving a sensitive measure of noise [45] .', 'As salicylate is added , mean MarA levels go up in the variants with negative feedback ( Fig . S2 ) .', 'For the systems without negative feedback both CV and noise strength are independent of salicylate concentration .', 'The combination of positive and negative feedback amplifies noise in the absence of induction , while allowing for tunable noise levels .', 'Histograms of MarA expression for the four network variants show that the Wildtype system produces a long-tailed distribution of MarA , while none of the other networks show this behavior ( Fig . S3 ) .', 'This subpopulation of cells with high MarA levels will induce resistance mechanisms , which can hedge against the sudden appearance of a stressor .', 'In the No Feedback and Only Positive variants , both CV and noise strength are constant , with positive feedback leading to higher noise ( Figs . 2C and D ) .', 'Interestingly , in the Only Negative case , the CV level depends upon induction , while noise strength does not .', 'This is because salicylate produces a reduction in active MarR2 levels , which is equivalent to a reduction in the MarR2-promoter association constant kr .', 'This parameter is independent of noise strength for a wide range of values in negative autoregulation [51] .', 'The decrease in Wildtype variability observed in Figs .', '2C and D arises from a disruption of stochastic pulsing and is not solely the result of an increase in MarA levels as the system is induced .', 'We next asked if the Wildtype dissociation constants we derived from the literature place the system in a favorable regime that minimizes the cost of expressing burdensome resistance machinery while maximizing the chance of survival in an uncertain environment .', 'To study this , we tested a range of association rates for MarA and MarR2 promoter binding while keeping the dissociation rate fixed and calculated both the cost of expressing MarA and the noise strength of MarA .', 'MarA induces many genes within the mar regulon that provide resistance to stressors , but expression of these genes is taxing to the cell [4] , [5] , [15] .', 'We calculated the cost of MarA expression by using the experimentally-derived function from [5] , which gives cost as a function of salicylate .', 'We related salicylate levels from this function to MarA expression directly by using data from previously published studies [5] , [12] ( Fig . S4 , Text S1 ) .', 'Increased positive feedback and decreased negative feedback strengths , given by association rates ka and kr , produce higher levels of MarA , which result in a higher cost .', 'By this metric , the Wildtype network is in a very low cost regime ( Fig . 3A ) .', 'We also calculated the noise strength as a function of the association rates , showing that a region of high noise strength exists when the association rates of the activator and repressor are balanced ( Fig . 3B ) .', 'The nominal feedback strengths of the Wildtype system place the system on a plateau of high noise strength , guaranteeing stochastic pulsing and relative insensitivity to feedback strength .', 'The curvature of the elevated noise strength regime is due to the nonlinear nature of the interactions between the binding of MarA and MarR2 to the promoter .', 'The high noise strengths observed when the MarR2–promoter association rate kr is low are the result of very slow fluctuations in MarA and MarR2 that keep the system far from the mean .', 'The Wildtype system is in a region with low cost and high noise strength .', 'This combination of conditions enables the creation of MarA pulses , which can trigger the induction of antibiotic resistance genes without undue burden to the population .', 'To study the robustness of our results , we conducted a sensitivity analysis for all model parameters to ensure that our findings were not specific to a particular set of values .', 'For equivalent systems with 2-fold increases and decreases relative to the wildtype parameters , we calculated the number of MarA pulses and the noise strength of MarA and compared them to the results observed in the original system ( Fig . S5 ) .', 'In all cases , results mirrored those from the original system with pulses in MarA observed with 0 mM salicylate but not with 5 mM salicylate .', 'Additionally , we calculated the noise strength for MarA , which showed similar results: noise strength is higher in the absence of salicylate .', 'The sensitivity analysis provides insight into the model parameters that have the largest impact on pulsing dynamics .', 'When the transcription , translation , or degradation rates are modified , the number of pulses and the noise strength are correlated with MarA levels .', 'In other words , when MarA levels go up due to changes in these parameters , MarA pulse numbers and noise strength increase; decreases result when the protein levels go down .', 'Antibiotics and other harmful compounds are ubiquitous in the environments where bacteria grow , however their appearance is often non-constant and time varying .', 'Such dynamic stress profiles have forced prokaryotes to develop mechanisms to protect themselves , including expression of pumps , superoxide dismutases , and other enzymes [52]–[54] .', 'Cells can take several approaches when expressing resistance genes .', 'First , they could always express the resistance genes ensuring that they will be prepared for the sudden appearance of a stressor , but the downside of this approach is that expression can be burdensome .', 'Alternatively , cells could induce resistance genes in response to a sensed stressor .', 'Finally , individual cells within a population could stochastically express resistance genes such that at any given time some cells in the population would be in a resistant state .', 'Bulk population studies have demonstrated that expression of MarA and subsequent resistance is inducible .', 'Here , we have shown computationally that in addition to this inducible resistance , expression of MarA can exhibit stochastic pulses when uninduced .', 'We asked what benefit the combination of stochastic pulsing and inducible resistance provides to cells .', 'For inducible resistance mechanisms , a system must respond to a sensed signal and turn on expression of resistance genes , thus , there is a delay between the time when a stressor appears and when the response in mounted .', 'Following induction with salicylate , maximal transcription of marRAB is observed after 30 minutes [55] .', 'MarA must activate downstream genes , further delaying appearance of the resistance phenotype , as demonstrated in experiments with the MarA homolog SoxS [56] .', 'Because expression of resistance mechanisms is not instantaneous with an inducible system , the system is vulnerable to the sudden appearance of a stressor .', 'Stochasticity in expression of MarA in the uninduced state would allow for some fraction of cells to always be in an elevated state of resistance , ready to counter the unexpected appearance of a stressor .', 'We hypothesized that tunable variability would increase survival in a time-varying stress environment .', 'To test this , we implemented a stochastic competitive growth assay to compare the fitness of the Wildtype network to a new variant with reduced noise ( Reduced Noise ) .', 'Competition assays can be used to discriminate between genotypes in order to identify those that achieve higher population fitness [57]–[59] .', 'We developed a stochastic competition assay by using a modified evolutionary algorithm: cells are first initialized with equal representation of each of the alternative networks , simulation are performed , the cost for each cell is calculated , and cells with poorly performing phenotypes are replaced by top performers ( Methods ) .', 'To allow for a controlled comparison between the networks , we required that the Reduced Noise network have the same mean MarA and MarR2 expression as Wildtype for all salicylate levels and the same response time after induction with salicylate when simulations are started from the same state ( Fig . S6 ) , satisfying the equivalence requirements from [60] .', 'The Reduced Noise network exhibits less variability than the Wildtype system , as shown in Fig . S6 , due to a reduction in the MarR2 inhibition constants and independent binding by MarA and MarR2 at the promoter ( Methods ) .', 'Therefore , the time scale and mean levels of the induced response are identical for both variants , while the stochastic response is attenuated in the Reduced Noise variant .', 'We found that the optimal strategy for surviving antibiotic stress depends on the frequency with which the stressor appears .', 'We first varied the probability of antibiotic addition in a time-varying stress profile ( Fig . 4A ) .', 'The Wildtype network outperforms the Reduced Noise network with large improvements coming when fluctuations in antibiotic levels jump from off to high in a short period of time .', 'When high antibiotic levels are preceded by a period of low or moderate antibiotics , the Reduced Noise network is at a slight advantage because the resistance genes are already induced for both variants and the variability is lower in the Reduced Noise case .', 'Fig . 4B summarizes the average response of the two networks as a function of the probability of antibiotic addition .', 'For time-varying stress profiles we found that phenotypic variability allows cells with the Wildtype network to outperform the Reduced Noise variant since they are able to survive sudden , large increases in antibiotic concentration .', 'In contrast , we found that when we competed the Wildtype and Reduced Noise variants in a constant environment the Reduced Noise variant outperformed the Wildtype system ( Fig . 4C ) .', 'In a constant environment there is no advantage to having variability in MarA and rises and dips will send the system into states that are more costly or less fit .', 'Findings from the constant environment demonstrate that the results shown in Fig . 4B are not the result of a systematic bias in favor of the Wildtype variant .', 'Instead , we find that the Wildtype variant outperforms the Reduced Noise system only in fluctuating , non-constant stress environments , suggesting that variability can be helpful under certain dynamic stress profiles .', 'Results from the competition simulations with time-varying stress show that variability in MarA is important for surviving the sudden appearance of antibiotics .', 'We asked whether stochastic pulses in MarA expression could be used as a bet hedging strategy by a population of cells .', 'To test this we simulated cells for an initialization period in the absence of antibiotics and then introduced a single pulse of antibiotic , quantifying the fraction of the population that was able to survive ( Fig . 4D ) .', 'As the magnitude of the antibiotic pulse increases , the fraction of cells that survived decreases .', 'However , the survival percentages depend upon how MarA expression is controlled .', 'When antibiotic pulses are of high magnitude , the Wildtype populations have some cells that are in a high MarA state and are able to survive the treatment .', 'Low amplitude pulses favor the Reduced Noise system because at any given time more cells are in a resistant state than with the Wildtype network where a larger range of MarA levels are sampled .', 'Those cells in a low MarA state do not have enough time to mount a response when the appearance of antibiotics is sudden .', 'Consequently , stochastic pulses help populations of cells to insure against the sudden appearance of an antibiotic where sensing-based mechanisms would be too slow to respond .'], ['The analysis presented here reveals how the combination of stochastic gene expression with inducible tolerance can serve to increase population-level survival in dynamic , time-varying stress environments .', 'We consider the regulatory network controlling expression of the multiple antibiotic resistance activator MarA , which regulates many downstream genes that confer tolerance to antibiotics and other inhibitors .', 'Previous studies have shown that expression of MarA can be induced by compounds like salicylate or through mutations that eliminate transcriptional repression of the marRAB operon [19] , [61] .', 'However , the regulatory topology that controls expression of marRAB consists of a pair of interlinked positive and negative feedback loops , begging the question what role this additional regulatory structure provides , given that simple negative feedback would be sufficient to allow for inducible expression of MarA .', 'Using a stochastic mathematical model , we studied the role of the feedback loops both separately and in combination .', 'Our findings suggest that the negative feedback loop alone can produce inducible expression of MarA that exhibits low amplitude variability when both MarR2 molecules unbind from the promoter .', 'Positive feedback serves to amplify this effect , creating stochastic pulses in MarA .', 'Furthermore , we find that the nominal system parameters derived from the literature place the marRAB network in a regime with high variability and low cost .', 'Thus , individual cells exhibit noisy MarA expression without an undue burden from expression of taxing resistance mechanisms .', 'Phenotypic heterogeneity in isogenic populations can provide a strategy for survival in uncertain environments .', 'Our modeling results predict that MarA expression exhibits stochastic pulsing when uninduced .', 'This variability , as measured using the coefficient of variation and noise strength , decreases as the system is induced .', 'In the induced state there is little need for variability and it may be detrimental , causing some cells to move into a regime with low stress tolerance or unnecessary cost .', 'Controlling for the mean levels of MarA expression and the timing of induction , we compared the fitness of two similar marRAB regulatory networks with differing levels of noise .', 'We found that under constant conditions , it is disadvantageous to have variable MarA expression; in contrast , when stress profiles are dynamic , increased variability places a fraction of the population in a state that can tolerate the sudden appearance of a stressor such as an antibiotic .', 'There are several possible extensions to the findings presented here .', 'For example , the cost of expressing MarA when the system is induced has an impact on the growth rate .', 'Previous studies have shown that this affects processes such as protein dilution , transcription , and gene dosage [62] , [63] , all of which will have an impact on the system dynamics by introducing an additional indirect source of feedback .', 'Other significant sources of feedback may also arise from changes in the nutrient environment or in expression of the proteolytic degradation machinery .', 'Empirical growth laws , such as those presented in [62] , [63] , could be used to extend the model to account for these growth rate effects .', 'In addition , it would be interesting to include the contributions of MarA homologs SoxS and Rob in our model to examine how crosstalk between the regulators affects the dynamics of MarA [14] , [64] .', 'Future studies to test our modeling predictions in vivo , are also of immediate interest .', 'For example , a reporter for MarA could be used to measure the dynamics of expression at the single-cell level .', 'These results could be compared to a synthetic gene network that exhibits external equivalence to the wildtype system , such as a network with only negative feedback that has the same dynamic range and induced MarA levels .', 'Populations of isogenic cells can exhibit phenotypic heterogeneity through a variety of dynamic processes .', 'In our model of the marRAB network we observe stochastic pulsing without induction , but decreased variability after expression of MarA is induced .', 'Allowing for tunable stochasticity can provide a flexible approach to stress tolerance .', 'This strategy of integrating dynamic behaviors may prove to be a general mechanism for hedging against environmental uncertainty while allowing for well-defined sensory mechanisms that behave in a deterministic fashion .'], ['An exact , stochastic model was implemented using the Gillespie algorithm [46] and custom analysis code .', 'Models are based on the processes described below , where the reaction rates and parameters are detailed and referenced in Text S1 and Table S1 .', 'The model treats cell growth and division implicitly unless otherwise noted , however results are similar when cell growth and division are explicitly modeled ( Text S1 , Fig . S7 ) .', 'Four alternative feedback combinations were created .', 'To allow for a controlled comparison , we fixed the mean expression of MarA such that it was the same for all networks when the systems were uninduced ( 0 mM salicylate ) .', 'This was achieved by modifying the transcription rate and MarA degradation rates , maintaining the parameters in realistic ranges .', 'Specifically , the differences between the four alternatives are:', '( i ) Wildtype: The operator region contains two identical binding sites for MarR2 and one for MarA .', 'A total of six promoter states are modeled with distinct association and dissociation rates ( see promoter dynamics above ) .', '( ii ) Only Positive: Both binding sites for MarR2 are eliminated ( kr\u200a=\u200a0 ) .', 'Thus , only the promoter states P00 and P10 are included in this model .', '( iii ) Only Negative: The binding site for MarA is eliminated ( ka\u200a=\u200a0 ) .', 'The promoter states P00 , P01 , and P02 remain .', '( iv ) No Feedback: The system has basal , constitutive expression ( kr and ka\u200a=\u200a0 ) .', 'P00 is the only promoter state available .', 'Details on parameters are given in Text S1 .', 'For all heat maps , the positive feedback loop strength and the negative feedback loop strength were systematically varied by modifying ka and kr in a range wide enough to include slow and fast transitions between promoter states .', 'For each point , we calculated the noise strength and cost , using the average of six independent replicates .', 'The cost and noise strength were calculated based on MarA levels , using data generated after the initial system transients .', 'The cost was calculated by using the function from [5] ( given by MCost below ) , calculating equivalent salicylate to MarA levels using data from the Hill function in Fig . S4 .', 'To calculate the number of pulses in MarA , we used the following heuristic: a pulse was defined as a period when MarA levels exceeded 2/3 of the 90th percentile of the number of molecules for at least 20 minutes .', 'Pulses separated by less than 15 minutes were combined into a single pulse .', 'We created a variant with the same MarA expression for all salicylate concentration , but which had reduced noise compared to the Wildtype network .', 'To achieve this , the inhibition constants , cInh1 and cInh2 , were decreased , causing higher minimum MarR2 levels , increasing the probability of binding to the promoter and stopping the pulse at an earlier stage .', 'In contrast to the Wildtype model , independent binding by MarA and MarR2 at the promoter is modeled , allowing MarR2 to bind easily when MarA is bound to the promoter .', 'The parameters modified for this variant are detailed in Table S3 .', 'To compare the two variants ( Wildtype and Reduced Noise ) in a head-to-head fashion , we simulated a competitive growth environment in the presence and absence of antibiotics .', 'The following procedure was used to model competitive growth: Step 1 .', 'Allow cells to grow without competition for an initialization period .', 'Step 2: Simulate all individual cells for a fixed time , given an identical antibiotic time course .', 'Step 3: Calculate the cost using the MarA levels for the cell .', 'The cost of growing for the cell is the sum of the cost of expressing the resistance machinery , measured as a function of salicylate , and the cost of growing with the antibiotic , minus their product ( Bliss independence is assumed [5] ) .', 'The effective concentration of the antibiotic is inversely related to the concentration of salicylate [5] .', 'For our experiments we used an compound that has the same cost for the cell as tetracycline and induces MarA with the same strength as salicylate , where both relations are defined in [5] .', 'Cells with costs above a threshold are determined to be dead and eliminated from the competition .', 'Competitive growth results are not sensitive to the exact value of this threshold .', 'Step 4: Calculate , for each cell , the number of replications and replace underperforming cells with those that are growing well .', 'Here , we take into account cell growth and division such that cells with lower costs are more prevalent than those with high costs .', 'The number of daughter cells for each variant is obtained and the ratio between variants is calculated .', 'This ratio is used to set the proportion between variants in the new population .', 'In other words , dead and underperforming cells are replaced by healthy cells such that the new proportion between populations is the same as the proportion between the growth of the old populations .', 'This allows us to maintain a constant number of cells , while at the same time representing the growth of the total population .', 'This process is then repeated by returning to Step 2 until a variant overtakes the population or a predetermined maximum number of rounds is reached .', 'Further details are provided in Text S1 .', 'Three com
['Introduction', 'Results', 'Discussion', 'Methods']
['Cells live in uncertain , dynamic environments and have many mechanisms for sensing and responding to changes in their surroundings .', 'However , sudden fluctuations in the environment can be catastrophic to a population if it relies solely on sensory responses , which have a delay associated with them .', 'Cells can reconcile these effects by using a tunable stochastic response , where in the absence of a stressor they create phenotypic diversity within an isogenic population , but use a deterministic response when stressors are sensed .', 'Here , we develop a stochastic model of the multiple antibiotic resistance network of Escherichia coli and show that it can produce tunable stochastic pulses in the activator MarA .', 'In particular , we show that a combination of interlinked positive and negative feedback loops plays an important role in setting the dynamics of the stochastic pulses .', 'Negative feedback produces a pulsatile response that is tunable , while positive feedback serves to amplify the effect .', 'Our simulations show that the uninduced native network is in a parameter regime that is of low cost to the cell ( taxing resistance mechanisms are expressed infrequently ) and also elevated noise strength ( phenotypic variability is high ) .', 'The stochastic pulsing can be tuned by MarA induction such that variability is decreased once stresses are sensed , avoiding the detrimental effects of noise when an optimal MarA concentration is needed .', 'We further show that variability in the expression of MarA can act as a bet hedging mechanism , allowing for survival in time-varying stress environments , however this effect is tunable to allow for a fully induced , deterministic response in the presence of a stressor .']
['Cells can sense their environment and respond to changes , however the sudden appearance of a stressor can be catastrophic if the time it takes to sense and initiate a response is slow relative to the action of a stressor .', 'A possible solution is to couple a sensory response with a stochastic , random approach .', 'In the absence of stress , a random subset of cells expresses resistance genes , ensuring that if a stressor appears there will be some cells that are able to survive and regenerate the population; once stress is sensed all cells should respond by expressing resistance genes .', 'Such an approach is particularly advantageous when resistance mechanisms are taxing to the cell because it limits their expression when no stress is present .', 'We studied this phenomenon computationally using a model of the multiple antibiotic resistance activator , MarA .', 'MarA controls over 40 resistance genes and can be induced by many harmful compounds .', 'We show that when uninduced , the gene regulatory network controlling MarA is capable of producing stochastic pulses that can serve to hedge against sudden changes in the environment with minimal cost to the population .', 'When induced , MarA expression is elevated and has low variability to ensure a uniform response .']
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