Highly Significant Antiviral Activity of HIV-1 LTR-Specific Tre-Recombinase in Humanized Mice
[['The introduction of highly active antiretroviral therapy ( HAART ) into clinical practice in the mid-1990s profoundly reduced morbidity and mortality among HIV-1-infected patients , changing an almost always fatal disease into a manageable chronic illness [1] .', 'However , HAART is costly and occasionally not well tolerated [2] , [3] .', 'Particularly long-term HAART is frequently accompanied by emerging new toxicities , resulting in secondary complications that include metabolic disorders ( e . g . diabetes , hyperlipidemia ) , osteoporosis , cardiovascular disease and chronic kidney disease ( reviewed in [4]–[6] ) .', 'Furthermore , large cohort studies demonstrated that the life expectancy of patients receiving HAART still remains considerably shorter than that of uninfected subjects ( recently reviewed in [7] ) .', 'Most importantly , the fact that HAART does not eradicate HIV and that treatment intensification , even when employing advanced drug regimens , fails to completely clear the virus ( reviewed in [7] , [8] ) highlights the urgency of pursuing new strategies to find a cure for HIV infection .', 'It is generally believed that the main hurdle to virus eradication is the persisting HIV-1 infection in latent reservoirs , particularly in memory CD4+ T cells ( reviewed in [9]–[15] ) .', 'Latently HIV-1-infected resting CD4+ T cells are apparently established early in infection .', 'One current strategy to eliminate this pool of long-lived cells aims to specifically activate the transcriptionally quiescent provirus ( i . e . the integrated replication-competent HIV-1 genome ) , for example by modifying its chromatin structure through histone deacetylase ( HDAC ) inhibitors ( reviewed in [12] , [13] , [15]–[17] ) .', 'Upon HDAC inhibitor-induced HIV-1 antigen expression , it is expected that these cells either experience HIV-1-induced cell death or are eliminated by cytotoxic T cells ( CTLs ) .', "It is fair to assume that such purging strategies would greatly benefit from a technology that can concurrently remove integrated HIV-1 from the pool of productively infected cells , thereby restoring , or at least improving the patient's immune function .", 'A novel strategy to remove integrated HIV-1 is based on a tailored site-specific recombinase ( Tre ) , derived by molecular evolution of the bacteriophage recombinase Cre [18]–[20] .', 'Tre targets a specific 34 bp sequence ( loxLTR ) derived from a primary HIV-1 strain [21] located in the proviral LTR regions , resulting in excision of the integrated proviral DNA from the genome of infected cultured cells [18] .', 'This process not only suppresses viral replication , but in theory may also help eradicate HIV from an infected individual ( reviewed in [22] ) .', 'Administering Tre-recombinase to patients will most likely require a gene therapy approach .', "In principle , genetic therapies against HIV either modify the patient's peripheral CD4+ T cells or patient-derived CD34+ hematopoietic stem cells ( HSC ) [23]–[25] .", 'It is anticipated that the former strategy would lead to beneficial antiviral , although transient effects .', "The latter application will presumably be the preferred strategy in Tre-based virus eradication approaches , since , in theory , it allows perpetual repopulation of the patient's hematopoietic system with Tre-expressing HIV-1 target cells .", 'These cells may be selected in vivo [26] , since upon de novo infection they are able to remove the integrated HIV-1 proviral DNA , and thus remain functionally immune competent .', 'Independently of the selected gene therapy strategy , and prior to its potential use in HIV-infected patients , vector technology has to be developed that allows safe and efficient gene transfer followed by reliable transgene expression in target cells .', 'Moreover , the absence of cytopathic and/or genotoxic effects upon vector-mediated Tre expression , and the accurate excision of HIV proviral DNA from chromosomal integration sites has to be demonstrated .', 'Finally , the antiviral effects of Tre-recombinase have to be shown in vivo , i . e . in an appropriate animal model for HIV-1 infection .', 'All these analyses will be of utmost importance for developing a potential Tre-based therapy to treat HIV infection .'], ['For delivery of Tre-recombinase an HIV-1-derived replication-incompetent lentiviral vector ( LV ) was constructed that provides high safety levels due to a split packaging system; the self-inactivating ( SIN ) vector design; and a sequence element introduced to improve transcriptional termination [27] , [28] ( Figure 1A ) .', 'To avoid transgene-related side effects , the gene sequence encoding Tre-recombinase was placed under the control of an engineered Tre-resistant tandem TAR repeat ( 2TAR ) , the cis-active target sequence of the HIV-1 Tat trans-activator [29] .', 'This not only limits Tre expression to HIV-infected cells , but a duplicated TAR element also positively influences internal LTR promoter activity in the presence of Tat ( Figure S1 in Text S1 ) .', 'Finally , constitutive expression of the GFP marker protein was facilitated by the PGK promoter ( Figure 1A ) .', 'First , we investigated whether Tre-recombinase introduced by the LV construct is faithfully expressed in HIV-infected cells , and in turn , excises integrated chromosomal proviral DNA .', 'We generated a reporter cell line , HeLa-smurf , which is stably infected with a replication-incompetent HIV-1 mutant with the env gene partially deleted and the nef open reading frame partially replaced by a marker gene ( blb ) ( Figure 1B ) .', 'Thus , Tre-mediated excision of the proviral genome results in loss of blue fluorescent protein ( BFP ) expression , which can be tracked by flow cytometry .', 'To monitor Tre activity , HeLa-smurf cells were transduced in triplicates with the GFP-expressing LV particles LV-Tre or a scrambled tre version , the Tre-negative control construct LV-Ctr , cultured for various time periods and analyzed for BFP and GFP expression .', 'After transduction , both lentiviral constructs produced a GFP/BFP double positive cell population ( see top right quadrants in Figure 1C , and plotted data in Figure S2 in Text S1 ) .', 'However , whereas the BFP/GFP double positive population of LV-Ctr-transduced cells subsequently remained stable over time , the BFP/GFP double positive population observed in LV-Tre treated cells started to decrease at 72 hours post transduction ( Figure 1C and Figure S2 in Text S1 ) .', 'Simultaneously , the GFP-only positive population increased , suggesting that Tre-mediated excision of HIV-1 proviral DNA has occurred .', 'Importantly , as the BFP/GFP double-positive population declined , expression of Tre-recombinase decreased , owing to the Tat-driven promoter providing temporal Tre expression ( Figure 1D ) .', 'We performed a panel of assays to verify Tre-mediated excision of the provirus .', 'First , we investigated the excised circular recombination product using a PCR assay ( Figure 2A ) .', 'An excision-specific fragment was only detected in DNA prepared from Tre-treated HeLa-smurf cells ( LV-Tre ) , but not in cells treated with the negative control vector ( LV-Ctr ) ( Figure 2B ) .', 'The recombination product appeared as early as 24 hours after transduction , and can be detected until 6 days post transduction ( p . t . ) time point .', 'As expected , the intensity of the PCR signal continuously increased , reaching a maximum at day 4 p . t . , before starting to decline ( Figure 2B ) .', 'The direct sequencing of the LTR and its flanking regions confirmed that Tre-mediated recombination of loxLTR sequences had occurred in a highly accurate fashion , exactly maintaining the 34 bp loxLTR Tre-specific target sequence ( Figure 2D ) .', 'Importantly , the time-based occurrence and intensity of the excised proviral DNA closely corresponded with the declining BFP signal ( Figure 1C and Figure S2 in Text S1 ) , and coincided with temporal Tre expression ( Figure 1D ) .', 'Second , we only detected the remaining single LTR ( i . e . genomic scar ) in Tre-treated cells by PCR [30] ( Figure 2C ) .', 'Third , we identified a total of three proviral integration sites in genomic DNA from transduced HeLa-smurf cells by nrLAM PCR and high throughput sequencing [31] ( Table S1 and Figure S3–Figure S5 in Text S1 ) .', 'As expected , proviral coding sequences ( LTR/blb ) , as opposed to genomic sequences ( LTR/int ) , were under-represented in Tre-treated cells compared to HeLa-smurf cells treated with the control vector as shown for one specific integration site by quantitative PCR ( Figure 2E–G ) as well as by integration site-independent next generation sequencing ( Table 1 ) .', 'Thus , LV-Tre represents an efficient HIV-1 specific expression system for excising integrated HIV-1 provirus from cells .', 'The expression of antiviral genes may induce undesired effects , which could compromise host cell function .', 'To analyze potential Tre-related cytotoxicities , Tre was overexpressed for a period of 15 weeks in Jurkat T cells; i . e . using the constructs LV-cTre and LV-cCtr in which the 2TAR promoter was replaced by the cellular EF1α promoter , permitting constitutive transgene ( Tre ) expression .', 'Analyses of cellular metabolic activity ( measured by an MTT assay ) , cell cycle progression ( determined by DNA staining with propidium iodide ) and apoptosis ( assayed by Annexin V staining ) did not reveal any deleterious effect of Tre expression on the host cells ( Figure 3A–3D , respectively ) .', 'This was also reflected by comparable cellular growth curves , independently of whether Tre-recombinase was expressed ( Figure S6 in Text S1 ) .', 'We also investigated possible effects of Tre expression on hematopoiesis and immune function .', 'We transduced primary human CD4+ T lymphocytes with LV constitutively overexpressing the GFP marker protein and either Tre or the negative control .', 'Subsequently , the GFP-positive cells ( ∼90% of the cultures ) were analyzed with respect to immune activation by FACS , multiplex cytokine-ELISA and IL4 and IFNγ Elispot assays ( Figure 3E–3G , respectively ) .', 'These analyses suggest that prolonged overexpression of Tre does not negatively affect cellular activation of primary lymphocytes .', 'We furthermore investigated the capacity of Tre vector-transduced CD34+ HSC to differentiate into various hematopoietic lineages using colony forming unit ( CFU ) assays .', 'In all experiments , Tre vector-transduced HSC maintained their capacity to differentiate into the expected lineages ( Figure 3H and 3I ) , with no significant differences from the controls .', 'For further analyses , Tre was constitutively overexpressed for 21 days in primary human CD4+ T cells and potential chromosomal alterations were subsequently analyzed by spectral karyotyping ( SKY ) [32] and array-comparative genomic hybridization ( array-CGH ) [33] .', 'The combination of these cytogenetic assays exhibited neither chromosomal translocations , nor variations in DNA copy-number changes ( Figure 4A and 4B and Figure S7 in Text S1 ) .', 'Thus , Tre appears to lack any obvious off-target activity in human cells , at least within the limitations of these experimental systems .', 'Next we investigated other potential undesired alterations due to Tre expression .', 'A recent transcriptome analysis using whole human genome microarrays found no differences between Tre-treated and untreated control cells [34] , indicating that the expression of Tre does not alter the expression profile of cells .', "To verify Tre's target specificity , the SeLOX algorithm [35] , a locus of recombination site search tool , was employed to scan the human genome for potential Tre target sites .", 'Seven independent sequences that occur in the human genome and display the highest sequence similarity to loxLTR ( 5–8 nucleotide mismatches in the specificity-determining loxLTR halfsites ) were tested as sites for Tre-mediated recombination in E . coli and HeLa cells ( Figure 5 ) .', 'No recombination ( above background signal ) was detectable after strong and prolonged expression of Tre in E . coli or HeLa , respectively , demonstrating that naturally occurring human loxLTR-like sites are not a substrate for the recombinase .', 'However , by screening the Los Alamos HIV sequence database ( http://www . hiv . lanl . gov/ ) , two additional independent clinical HIV-1 isolates were identified with subtle single nucleotide loxLTR variations that served as Tre targets ( Method S1 and Figure S8 in Text S1 ) .', 'These findings suggest that Tre does not alter or recombine human chromosomal sequences ( Figure 4 and Figure 5 ) but may recognize certain s\\ingle nucleotide alterations in the loxLTR site of a variety of clinical HIV-1 isolates .', 'In conclusion , the combined data indicate that LV-mediated expression of Tre-recombinase does not induce cytopathic effects in human hematolymphoid cells .', 'Two approaches , suggested as gene therapies against HIV [23] , [24] , were taken to test the ability of Tre to suppress HIV-1 infection in vivo .', 'In the first approach , human CD4+ T cells were isolated from buffy-coats and transduced with LV-Tre or LV-Ctr particles , routinely resulting in ∼60% GFP+ cells ( as measured by FACS; not shown ) .', 'Then 6-week-old Rag2−/−γc−/− mice were conditioned with clodronate , irradiated and transplanted with 3×106 cells of the transduced total cell pools , which were characterized by CCR5 surface expression ( Figure S9 in Text S1 ) .', 'Rag2−/−γc−/− animals lack B , T , and NK cells , can be engrafted with either CD4+ T cells or CD34+ HSC , and in both cases , support HIV-1 infection [36]–[39] .', 'The engraftment of human lymphocytes was verified at 8 to 10 weeks post transplantation by FACS analysis of PBMCs , determining the percentage of mouse CD45+ , human CD45+ , human CD4+ , and GFP+ cells .', 'To assess Tre activity , mice with ≥1% human CD45+CD4+GFP+ peripheral cells were then infected by intra-peritoneal injection of 100 ng p24 antigen of the replication-competent CCR5-tropic HIV-1 pNLT2env ( BaL ) mcherry , bearing loxLTR sites derived from the primary HIV-1 isolate TZB0003 [21] .', 'Since we wanted to monitor Tre-mediated antiviral effects as directly as possible , i . e . by analyzing plasma viremia , this challenge virus does intentionally not encode a functional Nef protein .', 'It is known that intact Nef depletes CD4+ T cells in humanized mouse models , thereby also indirectly affecting viral loads [40] , [41] .', 'Therefore , Nef-mediated pathogenic effects were not addressed in the following in vivo analyses .', 'Upon infection , viral loads , GFP+ cells and PBMC surface markers ( see Materials and Methods section ) were subsequently monitored over time .', 'Inspection of the data obtained from the individual animals revealed suppression of HIV-1 viremia in the plasma of the mice transplanted with Tre-transduced cells ( LV-Tre; animal T1–11 ) , but not in the negative control animals ( LV-Ctr; animal T12–18; Figure 6 ) .', 'Moreover , in contrast to LV-Ctr treated animals , the percentage of human CD45+CD4+ cells increased during the 16 week observation period in the mice that received Tre-transduced CD4+ T cells ( Figure 6 ) .', "It should be noted that the engraftment of immune deficient mice with human cells is to a significant extent donor dependent , a fact that impacts on the animals' infection rates .", 'Therefore , to obtain statistics , the HIV-1 RNA copies in the mice at week 2 post infection were set to 100% and the fold difference of the change in these baseline levels was followed over time .', 'This analysis revealed a highly significant reduction of the viral load ( p\u200a=\u200a<0 . 0001 , n\u200a=\u200a11; for statistical method see Materials and Methods section ) in mice transplanted with Tre-transduced cells , as opposed to animals that received cells transduced with the negative control vector ( p\u200a=\u200a0 . 0811 , n\u200a=\u200a7 ) ( Figure 7A ) .', 'Moreover , the increase in the percentage of human CD45+CD4+ cells , particularly in Tre-treated mice as opposed to control animals was clearly significant ( Figure 7B ) .', 'This trend was also seen when assessing the percentage of CD4+GFP+ cells ( Figure 7C ) .', 'At 16 weeks after infection , animals were euthanized and various tissues analyzed .', 'Immunohistochemistry on spleen sections clearly revealed reduced numbers of HIV-infected cells in a Tre-treated as compared to a control animal ( Figure 7D ) .', 'FACS analyses of single cell suspensions derived from bone marrow , liver , spleen and thymus showed an enrichment of CD4+GFP+ cells , particularly in animals that had received Tre-transduced cells ( Figure S10 in Text S1 ) .', 'In a second in vivo approach to study Tre-based antiviral effects , newborn Rag2−/−γc−/− mice were irradiated and transplanted by intrahepatic injection with 3×105 LV-Tre and LV-Ctr transduced human CD34+ HSC .', 'The transduction rate of these cord blood-derived hematopoietic cells typically resulted in ∼30% GFP+ cells ( not shown ) .', 'Engraftment was verified at 10 to 12 weeks post transplantation by FACS analysis of PBMCs ( determining the percentage of mouse CD45+ , human CD45+ , human CD19+ , human CD3+ , human CD4+ and GFP+ cells ) .', 'Generally , animals with ≥0 . 5% of CD45+CD4+GFP+ lymphocytes in their peripheral blood were challenged with HIV-1 as described above .', 'As shown , in all LV-Tre treated animals ( HSC1–10 ) , as opposed to mice that received LV-Ctr transduced HSC ( HSC11–18 ) , the individual viral load declined over time and the percentage of human CD45+CD4+ cells either increased or remained constant ( Figure 8 ) .', 'Subsequent statistical analysis at week 12 post infection revealed that mean viremia in mice that received LV-Tre transduced HSC was significantly diminished ( p\u200a=\u200a<0 . 0001 , n\u200a=\u200a10 ) compared to control ( i . e . LV-Ctr ) animals ( p\u200a=\u200a0 . 5377 , n\u200a=\u200a8 ) ( Figure 9A ) , indicating a progressive loss of viral loads in Tre-treated animals over time .', 'In contrast to the human CD4+ T cell transplanted animals , the percentage of CD45+CD4+ T cells did not change significantly in these mice ( Figure 9B ) .', 'This may be explained by the fact that only a fraction of the transplanted HSC ( ∼30% ) were Tre-transduced , and thus protected from HIV replication .', 'CD4+GFP+ cells were detected over the entire 12-week period , demonstrating the successful development of LV-transduced peripheral T cells in these HSC transplanted mice ( Figure 9C ) .', 'The immunohistochemical analysis of human CD3+ and HIV-1 p24 antigen expressing cells in lymph nodes of representative euthanized mice demonstrated that p24+ cells were distinctly depleted in mice that received LV-Tre transduced HSC ( Figure 9D ) .', 'Furthermore , FACS analysis of cell suspensions derived from bone marrow or spleen of these mice verified the presence of transgenic human cells representing various hematopoietic lineages , including CD4+ and CD8+ T lymphocytes , pre-B and activated B cells , cells committed to the monocyte/macrophage lineage , NK cells and NKT cells ( see Figure S11 in Text S1 ) .', 'Collectively , the in vivo experiments document the antiviral activity of Tre-recombinase at the organismal level .'], ['The clinical development of HAART has been one of the great successes in modern medicine .', 'However , the fact that HAART cannot eradicate HIV [7] , [8] makes investigating novel antiviral strategies a prerequisite for developing a future cure for HIV infection [7] , [9] , [12] .', 'In effect , gene therapy strategies represent a technology holding high promise for future antiviral disease treatments [22]–[25] .', 'Indeed , various RNA-based technologies are currently being investigated in vivo , including , for example , the expression of RNA aptamers , siRNAs and shRNAs , TAR decoys , and ribozymes [42]–[44] .', 'Moreover , the expression of membrane-bound fusion inhibitors is another appealing antiviral strategy [45] , [46] .', 'These approaches efficiently suppress virus replication , and thus reduce viral loads for extended periods of time .', 'Another promising strategy appears to be disruption of the CCR5 gene [47] , [48] , for example by expressing engineered zinc finger nucleases ( ZFN ) [20] .', 'In humanized mice transplanted with either CD4+ T cells or CD34+ HSC , ZFN-mediated CCR5 disruption has been shown to confer resistance to de novo infection by CCR5-tropic HIV-1 , thereby controlling virus replication [49] , [50] .', 'In contrast , an antiviral strategy based on Tre-recombinase is independent of virus coreceptor usage ( i . e . tropism ) and can target cells that are already infected with HIV [18] , [34] .', 'Importantly , Tre-mediated provirus excision allows reversal of HIV infection at the cellular level , thereby avoiding viral cytopathic effects ( e . g . effects associated with viral antigen expression ) and possibly restoring host cell function .', 'As shown here , Tre expression mediated highly significant antiviral effects , which were equally observed in animals engrafted with Tre-expressing CD4+ T cells or Tre-expressing CD34+ HSC .', 'With respect to future clinical studies , this Tre effect is particularly impressive , since the animals had been transplanted with unselected cell pools , where only a fraction of the T cells ( ∼60% ) or the HSC ( ∼30% ) harbored the Tre expressing lentiviral vector .', 'Apparently , Tre-mediated protection of only a subpopulation of HIV-1 target cells suffices to achieve significant antiviral effects in vivo .', 'This may be explained by the in vivo selection of gene vector-transduced cells as well as potential bystander effects [22]–[25] .', 'The results presented here suggest that Tre vectors are promising antiviral reagents for therapies based on the genetic modification of both peripheral T cells and hematopoietic stem cells .', 'Clearly , the procedure for ex vivo treatment of peripheral T cells is comparably less complicated , and aims at a functional cure by achieving long-term control of HIV , preferably in the absence of HAART [7] .', 'In contrast , the development of a sterilizing cure that eradicates HIV-1 from an infected organism , if achievable at all , most likely requires a highly complex strategy , involving the autologous transplantation of gene-modified HSC [22] , [25] .', "It is then expected that the elimination of all HIV-infected cells may eventually depend on reconstituting the patient's immune functions , a process that presumably requires additional and potentially gene therapy-unrelated approaches such as , for example , immune activation and/or purging strategies [51]–[54] .", "It is likely that such multi-pronged eradication approaches will benefit from Tre-mediated provirus excision in the patient's immune effector cells ( e . g . CD4+ T cells ) .", 'Important safety issues related to gene therapies are generally connected with potential cytopathic effects caused by the respective transgene and/or the vector technology used .', 'The latter was addressed here by using an advanced lentiviral SIN vector design where transgene expression was placed under the control of a Tat-inducible promoter , limiting its expression to HIV-infected cells .', 'This strategy circumvents a major shortcoming of various antiviral gene therapies , the continued expression of foreign transgenes [23] , thereby minimizing undesired transgene-related side effects such as immunogenicity .', 'Obviously , expression of Tre-recombinase from a Tat-inducible promoter presumably precludes provirus excision in latently infected cells .', 'It is therefore conceived that in clinical virus eradication approaches Tat-responsive Tre-expressing vectors will only be used in combination with purging drugs that , as previously shown , not only specifically activate the transcription of otherwise quiescent proviral genomes [55] , [56] , but , at the same time , will also enable Tat-mediated Tre expression from the vector used in the present study .', 'In this context it is also important to note that a recent study demonstrated that drug-induced purging alone does not result in the elimination of patient-derived infected resting CD4+ T cells , even when autologous CTLs were present [57] .', 'In fact , after virus reactivation these cells where only killed when the HIV-specific CTLs were pre-stimulated , suggesting that virus eradication depends at least on a combination of purging drugs with therapeutic vaccination strategies [57] .', 'It is expected that such an approach would further benefit from the inclusion of an additional anti-HIV gene therapy [25] , [58] , such as Tre-mediated provirus excision [22] .', 'It is also noted that recent computational modeling of HIV dynamics in the presence of a replication incompetent Tre-recombinase-expressing therapeutic vector suggested that such an approach may indeed clear all HIV from the system in the long term [59] .', 'Clearly , our study does not investigate the efficacy of Tre for latent proviruses .', 'This is a significant limitation that will be addressed in the future .', 'Particularly , it will be of interest to see whether a residual Tat level exists in latently infected cells that enables Tre expression by the current vector design .', 'Alternatively , Tat-independent vectors that employ drug-inducible promoters may permit conditional Tre expression in resting cells .', 'For example , advanced doxycycline-responsive promoter systems hold the promise to further increase biosafety of gene therapies by actively controlling transgene expression [60] .', 'In this context one may also conceive the direct delivery of Tre-recombinase into patients , for example by applying Tre-containing virus like particles [61] .', 'It is noted , that excision of proviral DNA by recombinant cell permeable Tre-recombinase has been already demonstrated in cell cultures [34] .', 'Thus , such advanced Tre delivery systems are conceived to play an important role in the future , particularly for targeting latently infected resting cells .', "Another safety aspect that should not be underestimated is based on Tre's pronounced target site specificity .", 'The fact that the site-specific recombination process mediated by such Cre-derived enzymes neither produces free DNA ends ( e . g . double-strand breaks ) nor requires additional host factors [62] , minimizes the oncogenic risk .', 'In agreement , the advanced molecular cytogenetic analyses presented here demonstrate the absence of Tre-related genome-wide off-target effects .', 'This distinguishes Tre-recombinase from CCR5-specific ZFNs , which may suffer from off-target cleavage specificities [49] , [63] , [64] .', 'Nevertheless , virus entry inhibition by CCR5 knockout represents a highly attractive antiviral strategy that may be exploited to its full extent when combined with Tre-recombinase technology , thereby not only blocking de novo infection but also targeting already infected cells for provirus excision .', 'The presented data suggest that antiviral gene therapies are feasible using conditionally expressed , engineered Tre-recombinases that precisely remove HIV-1 proviral DNA without cytopathic effects .', 'Antiviral in vivo activity was observed by transduction of both CD4+ T cells and CD34+ HSC .', 'Particularly the latter stem cell-based approach may be a valuable component of future eradication strategies to cure HIV [22] , [25] .', 'The fact that the current Tre-recombinase recognizes particularly HIV-1 subtype A isolates may limit its broad application .', 'However , the recent identification of highly conserved HIV-1 LTR sequences [65] in combination with a novel loxLTR search tool [35] now permits the engineering of advanced Tre-recombinases with activity against the majority of HIV-1 variants .', 'Clearly , it is not expected that HIV-1 can be eradicated by Tre activity alone .', 'As outlined above , future HIV eradication strategies are conceived to be a combination of various antiviral approaches ( e . g . drug-based and gene therapies ) , host immunity enhancing treatments ( e . g . therapeutic vaccination approaches ) , and purging attempts to overcome latency [51]–[53] .', 'In summary , our data support the notion that Tre-recombinase technology can be a valuable component of such a multi-tiered strategy to treat HIV-infected patients .'], ['The animal experiments were performed according to the guidelines of the German Animal Protection Law .', 'The experimental protocols were reviewed and approved by the relevant German authority , the local ethics commission ( Ärztekammer Hamburg; OB-050/07 and WF-010/2011 ) and the Freie und Hansestadt Hamburg , Behörde für Gesundheit und Verbraucherschutz ( Nr . : 63/09 and 23/11 ) .', 'The lentiviral ( HIV-1-based ) SIN vector backbone has been described previously [28] , [66] .', 'Briefly , the vector comprises self-inactivating long terminal repeats ( SIN LTRs ) ( ΔU3 , R , U5 ) , splice donor ( SD ) , splice acceptor ( SA ) , packaging signal ( Ψ ) , central polypurine tract ( cPPT ) , and the Rev response element ( RRE ) .', 'A post-regulatory element derived from woodchuck hepatitis virus ( PRE ) ensures efficient posttranscriptional RNA processing [67] , [68] and a duplicated simian virus 40 ( SV40 ) upstream polyadenylation enhancer element ( USE ) optimizes termination of transgene transcription [28] .', 'The transgene expression cassette includes either the open reading frame of Tre-recombinase ( tre ) [18] or a scrambled version , plus sequences encoding the enhanced green fluorescent protein ( egfp ) [28] .', 'The scrambled version serves as a negative control ( Ctr ) ; since all ATG start codons were replaced by TGA stop codons , and all GTG triplets by CCT , the respective mRNA cannot be translated into a protein .', 'The tre and scrambled sequence are under the control of a Tre-resistant variant of the HIV-1 NL4-3 LTR ( GenBank accession number M19921 ) , containing one or two TAR elements .', 'The TAR duplication ( 2TAR ) was generated by standard PCR technology using HIV-1 NL4-3 proviral DNA as the template for amplification .', 'Expression of egfp is under the control of the constitutive human phosphoglycerate kinase ( PGK ) promoter [27] , resulting in a dual-promoter vector design .', 'The proviral construct pNLT2ΔenvBLB was generated by replacing the BlpI×XhoI fragment of construct pNLT2ΔenvPuro [18] with the coding sequence for a fusion protein ( BLB ) composed of mTag-BFP ( Evrogen ) and blasticidin-S deaminase [69] , both linked by the linker sequence 5′-GCGCTAGGTGCTGCCGCCGGTGGT-3′ .', 'The proviral plasmid pNLT2env ( BaL ) mCherry , encoding CCR5-tropic replication-competent HIV-1 was constructed by inserting the env gene ( 2572 bp ) derived from plasmid pWT/BaL [70] ( NIH AIDS Research & Reference Reagent Program , Cat . No . 11414 ) into the previously described vector pNLT2ΔenvPuro [18] .', 'Puromycin resistance encoding sequences were replaced by the gene ( 711 bp ) encoding the autofluorescent protein mCherry , which was derived from the plasmid pRSET-mCherry [71] provided by Dr . Roger Y . Tsien , University of California San Diego .', "HeLa and 293T cells were cultured at 37°C and 5% CO2 in Dulbecco's modified Eagle medium ( DMEM; Biochrom ) containing 100 units/ml of penicillin and streptomycin ( PenStrep; Biochrom ) and 10% fetal calf serum ( FCS; Biochrom ) .", 'To generate HeLa-smurf cells , 2×105 HeLa cells were infected with pseudotyped pNLT2ΔenvBLB ( MOI 1 ) .', 'Cells were sorted for BFP-positive cells 1 and 3 weeks after infection and cultured in DMEM supplemented with 100 units/ml of PenStrep , 10% FCS and 5 µg/ml blasticidin ( Invivogen ) .', "HIV-1 pseudotypes and lentiviral particles for infecting cultured cells were produced by transient cotransfection of 2×106 293T cells with the lentiviral or proviral plasmid and the respective packaging plasmids [27] using polyethylenimine ( PEI ) as a transfection reagent according to the manufacturer's protocol ( Polysciences , Inc . ) .", 'In detail , 6 µg of pNLT2ΔenvBLB and 1 . 5 µg of pCMV-VSV-G [72] were used for transfecting the proviral construct , or 6 µg of lentiviral vector , 1 . 5 µg of pRSV-Rev [27] , 1 . 5 µg of pCMV-VSV-G [72] and 3 µg of pMDLg/pRRE [27] .', 'At 72 hours post transfection viral supernatants were collected and passed through 0 . 2 µm pore size filters to ensure removal of any viral aggregates .', 'Titers of lentiv
['Introduction', 'Results', 'Discussion', 'Materials and Methods']
['Stable integration of HIV proviral DNA into host cell chromosomes , a hallmark and essential feature of the retroviral life cycle , establishes the infection permanently .', 'Current antiretroviral combination drug therapy cannot cure HIV infection .', 'However , expressing an engineered HIV-1 long terminal repeat ( LTR ) site-specific recombinase ( Tre ) , shown to excise integrated proviral DNA in vitro , may provide a novel and highly promising antiviral strategy .', 'We report here the conditional expression of Tre-recombinase from an advanced lentiviral self-inactivation ( SIN ) vector in HIV-infected cells .', 'We demonstrate faithful transgene expression , resulting in accurate provirus excision in the absence of cytopathic effects .', 'Moreover , pronounced Tre-mediated antiviral effects are demonstrated in vivo , particularly in humanized Rag2−/−γc−/− mice engrafted with either Tre-transduced primary CD4+ T cells , or Tre-transduced CD34+ hematopoietic stem and progenitor cells ( HSC ) .', 'Taken together , our data support the use of Tre-recombinase in novel therapy strategies aiming to provide a cure for HIV .']
['Current antiretroviral combination therapy can efficiently suppress virus replication , but cannot eliminate HIV .', 'Therefore , no cure for HIV exists .', 'A main hurdle for virus eradication is seen in the existence of resting cells that contain integrated replication-competent , but temporarily silenced , HIV genomes .', 'Therefore , the most direct approach to eliminating virus reservoirs is to remove HIV genomes from infected cells .', 'As previous studies suggested , this may be achievable by Tre-recombinase , an engineered enzyme that can excise integrated HIV from host cell chromosomes .', 'The present work analyzes the expression of Tre-recombinase in human cells and demonstrates highly accurate Tre activity in complete absence of Tre-related cytopathic effects .', 'Furthermore , in vivo analysis of Tre-recombinase demonstrates highly significant antiviral effects of Tre in HIV-infected humanized mice .', 'The presented data suggest that Tre-recombinase might become a valuable component of a future therapy that aims at virus eradication .']
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