A Precisely Regulated Gene Expression Cassette Potently Modulates Metastasis and Survival in Multiple Solid Cancers
[['The accurate processing and integration of multiple external signals is a common feature of biological networks in normal health and complex disease .', 'As illustrated by the examples of oxygen handling [1] , energy control [2] , and ion homeostasis [3] , such accuracy frequently involves the precise coordination of multiple cellular pathways , and mechanisms for regulating and balancing opposing activities .', 'In cancer networks , many similar requirements for pathway balance are likewise found as successful tumorigenesis requires the robust integration of both pro- and anti-oncogenic pathways controlling cellular proliferation , apoptosis , motility , adhesion and senescence [4] , [5] .', 'The importance of balancing opposing activities in cancer is illustrated by genes such as HEF1 ( NEDD9 ) , a metastasis-related gene [6] and HMMR , a gene involved in centrosome formation ( Pujana et al . 2007 ) .', 'Either repression or overexpression of HEF1 can cause mitotic defects [7] , [8] , indicating that its activity in tumors requires tight regulation .', 'Similarly , subtle alterations of HMMR expression in normal mammary tissues may promote breast tumorigenesis , underscoring the need to keep the HMMR gene tightly regulated [9] .', 'Such findings support the notion that balancing the activity of positive and negative effectors is likely to be a central requirement of many cancers .', 'At the systems-level , pathway balance is often facilitated through the use of network structures [10] conveying robustness to random fluctuations and errors [11]–[14] .', 'However , the pivotal balancing role played by certain genetic components may at least partially explain why some networks also exhibit ultrasensitivity – a phenomena where small changes in activity at specific components can suffice to elicit qualitative changes in output [12] , [13] .', "Ultrasensitivity may contribute to a network's ability to rapidly respond to changing environmental and genetic conditions [15] , [16] .", 'Intriguingly , there is emerging evidence that certain cancers can also display ultrasensitivity .', 'Some remarkable examples include the dramatic responses of chronic lymphocytic leukemia cells to colchicines , occurring at concentrations 10 , 000-fold lower than that required for similar effects on normal lymphocytes [17] , [18] , and the striking clinical responses of certain solid tumors to targeted pathway inhibitors [19] .', 'From a therapeutic perspective , such ultrasensitive components could prove particularly appealing as drug targets , as even small alterations might prove sufficient to induce potent effects on tumor phenotypes such as tissue invasion and metastasis .', 'However , our current understanding of the role that ultrasensitivity plays in cancer is still far from complete .', 'Identifying additional genetic components regulating pathway balance in tumors might thus improve our ability to target critical control nodes in cancer networks .', 'As a general strategy to identify ultrasensitive components in tumors , we hypothesized that', 'a ) such components should be precisely regulated and thus exhibit restricted levels of expression variation in cancers; and', 'b ) subtle alterations in the expression levels of these components should induce or be associated with significant phenotypic changes .', 'We then applied these criteria to determine if such precisely-regulated genes might be inferred from databases of tumor gene expression profiles .', 'While several groups have compared the expression profiles of multiple tumor and non-malignant tissues [20] , [21] , to our knowledge , no study to date has systematically attempted to investigate the issue of precise gene regulation in tumors .', 'Employing a genome-wide computational strategy , we identified and robustly validated a novel “Poised Gene Cassette” ( PGC ) of genes undergoing precise regulation in a microarray database of human tumors from diverse tissue types .', 'Furthermore , subtle alterations in PGC expression were associated with significant and measurable alterations in important tumor phenotypes such as experimental metastasis and patient survival .', 'Our results thus suggest the existence of a generalized homeostatic mechanism in solid tumors for maintaining precise levels of PGC transcription , which may be important for various cancer-associated phenotypes , such as tissue invasion and metastasis .', 'Importantly , the approach described in this study is quite generalizable and can be applied to other diseases .'], ['We hypothesized that genes precisely regulated in cancer should exhibit a highly restricted level of gene expression variation across a large database of individual tumor gene expression profiles .', 'To investigate this , we generated gene expression profiles for 270 primary tumors from six tissue types ( breast , colon , liver , lung , oesophageal and thyroid ) using Affymetrix U133A Genechips .', 'For every gene , we computed gene expression coefficient of variances ( CV ) , where genes with small CVs are considered more tightly regulated than genes with large CVs .', 'We focused on the top 15% most tightly-regulated genes in tumors , corresponding to an empirical CV cut-off of 0 . 28 .', 'To identify genes whose tight regulation was tumor-specific , we used a second database of 71 adjacent matched non-malignant tissues ( “control” tissues ) to eliminate from this 15% genes that were also tightly regulated in non-malignant samples ( CV>0 . 3 ) .', 'The use of an absolute CV threshold is permissible , as the global distribution of expression CVs between tumors and controls were highly similar ( mean CVs were 0 . 46 and 0 . 45 for tumors and controls ) ( Figure 1A ) .', 'Using this criterion , we identified a “Poised Gene Cassette” ( PGC ) of 48 genes exhibiting highly restricted levels of expression variation in tumors ( Figure 1B ) .', 'The F-test , a statistical method for comparing the variation of different data sets , confirmed that each of the 48 PGC genes was indeed associated with significantly decreased expression variation in tumors relative to controls ( one tailed F-test , p\u200a=\u200a0 . 0001 to 4×10−14 ) .', 'We also varied the CV threshold between 0 . 26–0 . 3 ( +/−7% ) and repeated the analysis .', 'Similar results were obtained ( Table S1 ) , indicating that the identification of PGC is not dependent on a particular CV threshold .', 'We investigated whether the reduced expression variation of the PGC might be due to technical features of the Affymetrix platform or the composition of the initial training set .', 'We considered the possibility that the reduced variance of the PGC might be due to an overabundance of ‘poor quality’ probes , which might be expected to cross hybridize with multiple genes and hence generate higher background signals [22] .', 'However , an examination of a vendor provided list of questionable probes ( i . e . , with ‘_s_at’ and ‘_x_at’ suffixes ) , confirmed that the PGC was not significantly enriched in poor quality probes ( p\u200a=\u200a0 . 4 ) .', 'In addition , a comparison of the PGC genes against an in-house curated list of unreliable array probes based on sequence redundancy and repeat mapping [23] confirmed that unreliable probes were not overrepresented in the set of PGC genes ( p\u200a=\u200a0 . 8 ) .', 'To investigate the influence of normalization protocol on PGC discovery , we re-processed the training set using a different normalization method ( RMA , [24] , [25] ) .', 'In the RMA-normalized data , we found that 90% of the original PGC genes still exhibited decreased expression variation in tumors relative to controls ( i . e . , CV ( control ) >CV ( tumors ) ) ( Figure 2A ) .', 'Thus , the tumor-specific restricted expression variation of the PGC does not appear to be dependent upon a specific normalization technique .', 'The reduced variation of the PGC is also not due to an overrepresentation of either high-expressing or low-expressing genes .', 'As shown in Figure 2B , the PGC genes were equally distributed across a wide range of expression levels and not confined to either low or highly expressing genes in tumors or control tissues .', 'Thus , the reduced expression variation of the PGC in cancers is unlikely to be due to the PGC genes simply being either highly expressed , rendering the PGC distinct from some studies suggesting an inverse correlation between expression variation and absolute expression levels [26] .', 'Similarly , the PGC is also not biased in lowly expressed genes , consistent with our original selection criteria requiring these genes to be reliably detected in the majority of samples ( see Methods ) .', 'It is also important to note that the PGC genes do not exhibit significant differences in their absolute mean expression levels between cancers and normal tissues ( Figures 1B and 2B ) , but instead only differ in their levels of expression variation between cancers and normal tissues .', 'This observation , as well as others , also provides an argument that the PGC genes are unlikely to represent tissue-specific expression ( see Discussion ) .', 'The discovery of the PGC is also not influenced by the overrepresentation of breast tumors in our initial training set ( breast tumors comprised 68% of the training set ) .', 'Specifically , we removed all the breast tissues and repeated the PGC analysis .', 'Even without inclusion of breast tissues , 83% ( 40/48 ) of the PGC genes still exhibited reduced variation in tumors compared to controls .', 'Of 47 genes exhibiting tumor-specific tight regulation in the breast-excluded data ( CV<0 . 28 ) , 24 genes were part of the original PGC , an overlap far beyond random chance ( 50% , p\u200a=\u200a1 . 3E-11 , hypergeometric test ) .', 'Taken collectively , these results suggest that the identification of the PGC , and its restricted expression variation in cancers , is unlikely to be due to a technical artifact or the inclusion of a specific cancer type .', 'To confirm that the restricted expression variation of the PGC was specifically associated with malignancy , we determined the frequency at which a member gene of the PGC could be re-identified in a series of class-permutation tests .', 'When the class labels of the samples ( i . e . , tumor or control ) were shuffled to generate a series of 1000 permuted sets , almost all the PGC genes ( 46/48 , 96% ) could only be re-identified in less than 5% of the class-permuted signatures , consistent with the decreased expression variation of the PGC being tightly associated with tumor samples .', 'We then evaluated the robustness of the PGC by repeated random sampling ( RSS ) , a stringent cross-validation strategy [27] .', 'The original training set was randomly divided 1000 times into two parts , generating a large series of distinct training/test set combinations .', 'For each of the 1000 derived RSS training sets , we identified new PGC signatures ( rPGC ) and compared them to the original PGC gene set .', 'Following the guidelines of Michels et al [27] , 20 genes were repeatedly selected in more than half of the 1 , 000 new rPGC signatures .', 'Of these 20 genes , 19 ( 95% ) are members of the original 48-gene PGC ( Figure 2C ) – the observation that only one gene not part of the original PGC signature was repeatedly selected in the RSS assay indicates that a substantial proportion of the PGC signature ( 40% ) is robust to training set selection .', 'To evaluate the transportability of the PGC signatures , we then applied each of the 1000 rPGC signatures to their cognate test sets .', 'In anticipation that most independent test sets are likely to contain either tumor or control samples but not both , we considered the tumors and controls separately from one another in this analysis .', 'In each test set , we checked if the population of tightly regulated genes , defined using the original CVT threshold ( 0 . 28 ) , contained a significant enrichment of rPGC genes ( see Methods ) .', 'The rPGC signatures were significantly enriched in the population of tightly regulated genes in 80% of the tumor test sets ( PGC→T , Figure 2D ) , and importantly were NOT significantly enriched in 100% of the control test sets ( PGC→N , Figure 2D ) , indicating that the PGC is robust in recapitulating its precise regulation in multiple tumor data sets , but not data sets of non-malignant samples .', 'Together , these results confirm the specificity of the PGC for tumors .', 'We then asked if the precision of PGC regulation in cancer could be observed in independent data sets of diverse tumors .', 'We collected nine independent cancer cohorts , comprising in total 1105 cancer samples from >7 primary tissue types [28]–[32] , including I ) four tissues not represented in the original training data ( gliomas , gastric , NPC , and ovarian ) , II ) one data set ( Yu_Gastric&NPC ) representing a mix of two different tissues , and III ) a collection of cancer cell lines ( NCI60 ) from nine different tissues .', 'A summary of these nine data sets can be found in Table S2 and the corresponding references .', 'Using a similar strategy to the RSS test sets , a significant fraction of the PGC genes were tightly regulated in all nine primary tumor data sets ( p-value range: 0–0 . 002 ) ( Table 1A ) , confirming the existence of the PGC in a wide variety of solid tumors .', 'In total , 19 out of 48 PGC genes repeatedly exhibited reduced expression variation in more than half of the 9 cancer test sets ( Table S3 ) .', 'We also performed the reciprocal experiment and evaluated the regulation of the PGC in a series of independent non-malignant samples .', 'Although such datasets are rarer in their availability and typically smaller than cancer datasets , we collected two distinct cohorts comprising 115 normal tissues from various organs [33] , [34] .', 'Notably , these non-malignant samples were obtained from healthy donors , and are thus free of malignancy and representative of true normal samples .', 'In stark contrast to the cancer data sets , the PGC genes exhibited either no or only a marginal degree of tight regulation in the normal data sets ( p\u200a=\u200a0 . 07 and 0 . 01; Table 1B ) .', 'Thus , these results indicate that the precise regulation of the PGC genes is largely restricted to cancer tissues , suggesting that diverse tumor types may harbor a general requirement for tightly regulating PGC expression .', 'A pathway analysis revealed multiple highly significant interactions between the PGC genes and prevalent tumorigenic pathways .', 'The top-scoring molecular network for the PGC comprised 11 PGC focus genes interacting either directly or indirectly with the well-known cancer-related transcription factors Myc and TP53 ( p\u200a=\u200a10−19 , see Methods ) ( Figure S1 ) , and the most significantly enriched cellular functions in the PGC were cancer ( p<0 . 0045 ) , tumor morphology ( p<0 . 0045 ) and cell cycle control ( p<0 . 0045 ) .', 'The PGC was also significantly enriched in components related to integrin signaling ( p\u200a=\u200a2 . 33E−04; Figure S1 ) , a complex signaling pathway implicated in both positive and negative regulation of tumor cell growth and cancer metastasis .', 'Besides integrin signaling , other individual PGC genes , such as RPS2 and RPL7A , have also been previously implicated in the control of cellular transformation , tumor growth , aggressiveness , and metastasis [35] , [36]; while the PGC gene MUS81 has recently been reported to interact with p53 to maintain genome stability [37] .', 'Thus , an array of biological and functional evidences suggest that the PGC genes are likely to be involved in the activity of multiple cancer-related pathways , and not ubiquitous ‘housekeeping’ cellular functions .', 'The full list of PGC genes is provided in Table S3 .', 'The tightness of PGC regulation in tumors might be explained if small alterations in the expression levels of these components are sufficient to cause significant phenotypic changes in tumors .', 'We employed three experimental assays to address this possibility .', 'First , we analyzed a set of colon cancer cell lines derived from either primary tumors or distant metastases from the same patient ( SW480 and SW620 ) , which have been shown to exhibit several phenotypic differences including metastatic potential [38] , [39] .', 'Using Gene Set Enrichment Analysis ( GSEA , [40] ) , we found that PGC expression was subtly yet significantly decreased in highly metastatic SW620 cells compared to poorly metastatic SW480 cells ( p<0 . 001 , Table S4 ) .', 'Second , we then analyzed patterns of PGC expression in a cohort of 30 breast cancer cell lines , where the invasive capacity of each line had been previously measured by matrigel assays [32] .', 'The PGC genes exhibited minimal expression variation across the lines when assessed using a standard range of expression variation , consistent with their being tightly regulated in cancers ( Figure 3A , left heat-map ) .', 'However , when the scale of variation was amplified , we identified by hierarchical clustering two groups of cell lines showing either subtly higher or lower levels of PGC expression ( Figure 3A , right heat-map ) .', 'Importantly , we again found that the majority of cell lines with high to moderate invasive abilities exhibited subtle yet significant decreased expression of the PGC genes compared to poorly invasive lines ( p\u200a=\u200a0 . 04 , chi-square test , sample groups defined on the basis of the top-level branch point ) .', 'To validate the robustness of this clustering by an alternative method , we then also performed independent k-means clustering ( k\u200a=\u200a2 ) .', 'Using k-means , 7 out of 8 highly invasive cell lines were clustered into one group together with 4 marginally or non-invasive cell lines ( p\u200a=\u200a0 . 01 , chi-square test for high vs . marginal/non-invasive ) , consistent with the groupings observed by hierarchical clustering .', 'Third , we conducted in vivo experiments using a murine xenograft model of metastasis , where poorly metastatic HCT116 colon cancer cells were injected into the spleens of nude mice , and metastatic liver tumor nodules were harvested 6 to 8 weeks later .', 'The liver nodules were expanded in culture and re-passaged in mice to generate a panel of lines ( M1 , M2 , and M3 ) with increasing levels of metastatic capacity ( Figure 3B ) .', 'Examining the gene expression profiles of these lines , we found that highly metastatic cells once again exhibited subtly decreased PGC expression compared to poorly metastatic HCT116 cells ( p\u200a=\u200a0 . 03 , Figure 3B and Table S4 ) .', 'These results , based on three different experimental models of metastasis , collectively suggest that small alterations in PGC expression in tumors may be associated with potent differences in tumor physiology , specifically metastatic and invasive capacity .', 'To directly demonstrate the functional role of PGC genes in cellular invasion , we performed siRNA experiments where five PGC genes ( p53CSV , MAP3K11 , MTCH2 , CPSF6 , SKIP ) were silenced in poorly-metastatic AGS gastric cancer cells .', 'While p53CSV is a gene required for p53-mediated cell survival [41] , its role in cancer is otherwise poorly understood .', 'Furthermore , associations between MAP3K11 , MTCH2 and CPSF6 to cancer have also not been previously reported .', 'The siRNA treatments reduced the expression levels of these five PGC genes from 45%–80% , as assessed by quantitative real-time PCR ( Figure 4A ) , and reductions in p53CSV , MAP3K11 , MTCH2 and CPSF6 resulted in a significant enhancement of in vitro invasive activity as measured in a matrigel assay ( p<0 . 01 , one-tailed t-test , Figures 4B and 4C ) .', 'Furthermore , SKIP siRNA treatment resulted in a significant inhibition of cellular proliferation in AGS cells ( p<0 . 01 , Figure 4D ) .', 'It is worth noting that for at least two genes ( p53CSV and CPSF6 ) , a partial reduction of gene expression of 45–60% was able to trigger a significant change in invasive phenotype .', 'To further demonstrate the generality of this phenomenon , we then knocked down p53CSV in another poorly-metastatic colon cancer cell line , HCT116 which we previously utilized in the xenograft assay .', 'Again , the partial silencing of p53CSV expression significantly increased the invasion activity of HCT116 cells ( Figure S3 ) .', 'These results suggest that the PGC genes may play roles in regulating cancer invasion and metastasis .', 'To extend the potential role of precise PGC regulation to the clinical context , we asked if similar small changes in PGC expression might be associated with significant differences in patient survival and clinical outcome .', 'We employed hierarchical clustering to group the tumors in each of the six data sets with survival data available by their overall level of PGC expression .', 'A representative example is shown in Figure 5A .', 'Once again , the PGC genes exhibited minimal expression variation across the tumors when assessed on a standard scale of expression variation , consistent with their being tightly regulated in tumors ( Figure 5A , left heat-map ) .', 'However , when the variation scale was amplified , we identified two groups of tumors showing either subtly higher or lower levels of PGC expression ( Figure 5A , right heat-map ) .', 'Remarkably , a Kaplan-Meier survival analysis revealed that in all six data sets , patients with tumors expressing PGC levels below the population average experienced significantly worse survival outcomes compared to patients with high-PGC expressing tumors ( Figure 5B; all cases p<0 . 05 except in ovarian cancer set where p\u200a=\u200a0 . 057 , see Figure S4 for clustering groupings ) .', 'We only observed comparable survival stratifications across the six data sets in 46 out of 10 , 000 randomly selected 48-member gene sets , arguing that the prognostic ability of the PGC is statistically unique .', 'In a multivariate analysis , PGC expression behaved as an independent prognostic factor compared to other clinical variables in the breast and colon cancer cohorts , and was associated with tumor stage in ovarian , lung and glioma cancer patients ( Table S5 ) .', 'Importantly , the PGC exhibits very little overlap with other expression signatures reported to predict clinical behavior in multiple tumor types .', 'A comparison of the PGC against a 128-gene metastasis signature ( MS ) [42] , a 70-gene chromosomal instability signature ( CIN70 ) [43] , a cell cycle module [44] , a wound response healing signature [45] , [46] , and multiple cell proliferation-related signatures ( 57–59 ) including a 874-gene cell cycle gene signature ( CPS ) [47] , revealed that there was no direct overlap in gene content between the PGC and these other “multi-tumor” gene signatures , except for a one-gene overlap with the CIN70 , and a four-gene overlap with the CPS , which was not statistically significant .', 'This finding suggests that the specific gene content of the PGC is distinct from other previously described signatures .', 'To ask if the PGC might target the same “poor prognosis” tumors as other published signatures capable of predicting clinical outcome in multiple tumor types , we then investigated the ability of the MS , CIN70 , and CPS to stratify patient survival in the six data sets - none of these signatures exhibited comparable prognostic significance to the PGC across the six patient cohorts ( data not shown ) .', 'These observations suggest that the PGC is likely to target different molecular features and types of tumors than the aforementioned signatures .', 'Previous gene expression studies comparing tumors and non-malignant tissues have typically employed microarray analysis algorithms such as t-tests with false positive correction or SAM [20] .', 'Genes detected by such techniques typically require both differing mean expression levels and equivalent levels of variation between two cellular states ( Figure S5 ) .', 'However , the PGC might not be detected by such conventional techniques , as PGC genes might not exhibit distinct mean expression levels between the two groups and only be associated with differing degrees of expression variation between tumors and controls ( Figure S5 ) .', 'Indeed , performing SAM and t-tests on the training set only identified 27% of the original PGC , after multiple hypothesis correction , and the absolute mean expression levels of many PGC genes between tumors or non-malignant tissues were highly similar ( Figure S5 ) .', "To ask if the unequal distributions in expression variation might underlie the failure of the PGC genes to be identified by conventional techniques , we also analyzed the original training data set using Welch's test , an adaptation of Student's t-test intended for use with two groups having unequal variance .", "Again , 75% of the PGC genes failed to be detected as significant using Welch's test ( data not shown ) .", 'These findings suggest that conventional algorithms would likely have failed to detect the PGC , thereby providing a partial explanation as to why the PGC might have been missed in previous studies .'], ['In this study , we identified a novel cassette of genes exhibiting tumor-specific precise regulation in multiple cancer tissues .', 'Our ability to discern the PGC was facilitated by the use of an analysis method focused on expression variance rather than expression levels .', 'The reduced variance of the PGC in tumors is unlikely to be a technical artifact of the Affymetrix platform , as it was not related to probe selection , data normalization , absolute high or low expression levels in either tumors or non-malignant tissues , or sample set .', 'Using both rigorous cross-validation ( RSS ) and multiple independent validations , we found the PGC to be robust to alterations in training set composition and repeatedly observed in diverse malignant tumor types , including several tissue types not present in the original training data .', 'Importantly , the PGC failed to demonstrate tight regulation in several non-malignant tissue data sets , arguing that its control is cancer-specific .', 'Interestingly , even though it was not a specific requirement in our initial analysis , the majority of PGC genes exhibited similar mean expression levels in both tumors and non-malignant tissues .', 'This absence of a distinct difference in mean expression values resulted in the failure of standard microarray analysis methods ( e . g . , t-test ) to detect the majority of PGC genes when applied to the same training data set .', 'Furthermore , a standard practice in microarray data processing is to filter out genes exhibiting low variation prior to clustering or statistical analysis - such filtering would inevitably lead to a bias towards differentially expressed genes and prevent the discovery of the PGC .', 'One potential concern might be that the PGC genes simply reflect the activity of tissue-specific gene expression .', 'However , five findings argue against this possibility .', 'First , while dedifferentiated cancer cells frequently exhibit a loss of tissue-specific gene expression ( Rhodes et al . 2004 ) ; such a loss would typically result in tissue-specific genes being down-regulated in their absolute expression levels compared to normal tissues .', 'In contrast , the PGC genes do not exhibit significant differences in their absolute expression levels between cancers and normal tissues ( Figure 2B ) .', 'Second , the reduced variation of the PGC genes was consistently observed in multiple independent sets from diverse tissues ( e . g . gliomas , lung , breast ) , including a data set ( NCC ) that combined tissues from two different sources ( gastric and NPC tumors ) .', 'Third , the PGC genes also showed reduced expression variation in the NCI60 test set - a mixture of cancer cell lines from 9 different tissue types .', 'Fourth , the PGC genes consistently exhibited reduced expression variation in the repeated random sampling ( RSS ) cross-validation assay , where we tested 1000 distinct training set and independent test sets composed of mixed tissue types ( Figure 2D ) .', 'Fifth , even within each of the six tissue types in the training set ( liver , colon , esophagus , thyroid , lung , and breast ) , the majority of the PGC genes ( 70% ) are not differentially expressed within tumors and normals ( p>0 . 01 , t-test ) ( YK , data not shown ) .', 'Taken collectively , it is unlikely that the consistency of the PGC would have been observed if its reduced expression variation was solely due to tissue-specific expression , supporting the notion that the PGC genes are likely to be distinct from the conventional differentially expressed gene signatures described in most microarray studies .', 'One possible explanation for why certain genes may require precise control is if they regulate or are involved in balancing disparate downstream pathways possessing mutually opposing activities .', 'In cancers , the successful establishment of a malignant tumor involves multiple pro- and anti-oncogenic forces involved in cell proliferation , apoptosis , cell death , senescence , cell adhesion , and motility , all of which require delicate balance by different genetic components .', 'For example , while loss of Ras signaling is lethal , aberrant signaling through this pathway is important for cancer development but can also drive cells into either senescence or cell proliferation , depending on cellular context [48] , [49] .', 'Another good example is the anti-apoptotic gene Akt/PKB ( protein kinase B ) , which when constitutively activated reduced metastases in mice and inhibited the invasion of breast cancer cells [50] , [51] , indicating its involvement in multiple cancer pathways .', 'Reassuringly , similar examples of balanced coordinator genes are also seen in the cohort of PGC genes .', 'The PGC gene FUS1 ( also known as FUS ) has been reported as a tumor suppressor gene in lung and breast cancer [52] and a pro-oncogene in leukemia [53] .', 'Oxidative stress , which may play an important role in cancer progression and the regulation of cancer metastasis [54] , is dependent upon the critical balance between intracellular hydrogen peroxide H2O2 and superoxide O2− .', 'Two PGC genes - p53CSV and KIAA0247 have been reported to be induced in response to oxidative stress [55] , and may influence this balance and the response of tumor cells to apoptotic stimuli [56] .', 'It is also worth noting that the PGC was significantly overrepresented in components of the integrin signaling pathway – a highly complex process involving multiple related family members with roles in many cellular functions , including ERK/MAPK and JNK/SAPK regulated gene expression , cell motility , cytoskeletal interactions , and PI3K and Wnt pathway signaling [57] .', 'In metastasis , integrins are crucial for cell invasion and migration , not only for physically tethering cells to the matrix , but also for sending and receiving molecular signals regulating these processes [57] .', 'Moreover , while some groups have proposed that increased integrin expression could promote malignant behavior by enhancing tissue stiffness [58] , other groups have suggested that loss of integrins may promote tumor invasion and metastasis [59] .', 'The complexity of integrin family members and their pathway components also provides a plausible explanation for why even subtle alterations in PGC expression are associated with distinct and measurable changes in metastatic behaviour in both experimental models of metastasis and clinical outcome .', 'What might be the mechanistic basis of precise PGC regulation ?', 'At a general level , many precisely-regulated genes are likely to possess complex regulatory systems for tightly controlling expression levels , to rapidly sense and adapt to dynamic perturbations in both the internal and external environment [60] .', 'Such mechanisms could involve the use of both positive and negative feedback loops , analogous to the circuitry utilized by the LacI/O bacterial system to ensure precise expression [61] , but in cancers could also involve eukaryote-specific mechanisms like epigenetic modifications ( DNA methylation or chromatin modifications ) , microRNA regulation , or transcription factor binding .', 'Interestingly , in a preliminary analysis , we attempted to extend our observations from the pathway analysis showing an association of several PGC genes with both Myc and TP53 .', 'Specifically , we in
['Introduction', 'Results', 'Discussion', 'Materials and Methods']
['Successful tumor development and progression involves the complex interplay of both pro- and anti-oncogenic signaling pathways .', 'Genetic components balancing these opposing activities are likely to require tight regulation , because even subtle alterations in their expression may disrupt this balance with major consequences for various cancer-associated phenotypes .', 'Here , we describe a cassette of cancer-specific genes exhibiting precise transcriptional control in solid tumors .', 'Mining a database of tumor gene expression profiles from six different tissues , we identified 48 genes exhibiting highly restricted levels of gene expression variation in tumors ( n\u200a=\u200a270 ) compared to nonmalignant tissues ( n\u200a=\u200a71 ) .', 'Comprising genes linked to multiple cancer-related pathways , the restricted expression of this “Poised Gene Cassette” ( PGC ) was robustly validated across 11 independent cohorts of ∼1 , 300 samples from multiple cancer types .', 'In three separate experimental models , subtle alterations in PGC expression were consistently associated with significant differences in metastatic and invasive potential .', 'We functionally confirmed this association in siRNA knockdown experiments of five PGC genes ( p53CSV , MAP3K11 , MTCH2 , CPSF6 , and SKIP ) , which either directly enhanced the invasive capacities or inhibited the proliferation of AGS cancer cells .', 'In primary tumors , similar subtle alterations in PGC expression were also repeatedly associated with clinical outcome in multiple cohorts .', 'Taken collectively , these findings support the existence of a common set of precisely controlled genes in solid tumors .', 'Since inducing small activity changes in these genes may prove sufficient to potently influence various tumor phenotypes such as metastasis , targeting such precisely regulated genes may represent a promising avenue for novel anti-cancer therapies .']
['Successful carcinogenesis involves the integration of both pro- and anti-oncogenic pathways .', 'We postulated that genes critical for balancing these opposing pathways are likely to be precisely controlled in tumors , since even subtle alterations in their activity might cause substantial alterations in tumor growth and survival .', 'Using a novel genomic approach , we identified a 48-gene “Poised Gene Cassette” ( PGC ) showing tight regulation specifically in human cancers but not in corresponding nonmalignant tissues .', 'We show , using a wide variety of in vitro and in vivo approaches , that small alterations in PGC expression are consistently associated with significant differences in experimental metastasis and patient survival , and we demonstrate a direct functional role for five PGC genes ( p53CSV , MAP3K11 , MTCH2 , CPSF6 and SKIP ) in cancer invasion .', 'Our findings support the existence of a novel class of ultrasensitive genes that may regulate various cancer-associated phenotypes such as metastasis .', 'Such precisely controlled genes could represent appealing drug targets , since even partial alterations in their activity should prove sufficient to induce potent effects on tumors .', 'Besides cancer , our analytical approach is quite generalizable and likely to be applicable to other disease conditions .']
['molecular biology/bioinformatics', 'oncology', 'computational biology/genomics', 'genetics and genomics/gene expression']